CN103548878A - Leaf-vegetable immunizing agent and application thereof - Google Patents
Leaf-vegetable immunizing agent and application thereof Download PDFInfo
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- CN103548878A CN103548878A CN201310554240.1A CN201310554240A CN103548878A CN 103548878 A CN103548878 A CN 103548878A CN 201310554240 A CN201310554240 A CN 201310554240A CN 103548878 A CN103548878 A CN 103548878A
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Abstract
The invention relates to a leaf-vegetable immunizing agent and an application thereof, which belongs to the field of crop production. Aiming to solve the technical problems, the invention provides a leaf-vegetable immunizing agent and an application thereof. The leaf-vegetable immunizing agent provided by the invention comprises the following active components in parts by weight: 1-6.4 parts of chitosan and derivatives thereof, 0.4-4 parts of nitrogen, 0.3-1.0 part of phosphoric acid and 0.8-5.0 parts of potassium chloride, wherein the chitosan and the derivatives thereof are water-soluble chitosan and derivatives thereof; nitrogen is nitrogen elements and components which do not react with the water-soluble chitosan and the derivatives thereof for generation of a precipitate, and the mass of nitrogen is based on nitrogen elements. The invention also relates to an application of the leaf-vegetable immunizing agent. The components of the leaf-vegetable immunizing agent are harmless to human body and environments, and the leaf-vegetable immunizing agent does not cause hormone residues, is low in production cost, and meanwhile has the advantages of good effects on improvement of crop immunity and the like.
Description
Technical field
The present invention relates to leaf vegetables immunizing agent and application thereof, belong to crops production field.
Background technology
Along with the continuous increase of China's population, how the fast development of industry and the continuous quickening of urbanization process, and the minimizing of ploughing, improve crop immunity, reduces the possibility that disease occurs, and improves the output of crop, is the emphasis direction of agricultural research.
Leaf vegetables immunizing agent can increase the resistance of crop to disease, can make to a certain extent crop away from the generation of disease.Leaf vegetables immunizing agent also has application in agricultural at present, and more common forms for number of chemical composition is composite, and complicated component the safety of crop is needed to be inquired into, and application cost is higher; Also have the immunizing agent that adopts plant growth regulator to be composited, but somatotropin is residual, has caused again new problem; Simultaneously, also have and adopt the immunizing agent being prepared from through special zymotechnique by the useful interior raw symbiosis bacillus of plant, be plant biological and ecological methods to prevent plant disease, pests, and erosion immunizing agent technology, but it need to expend the screening that a large amount of workloads is carried out bacterial strain, and large-scale fermentation equipment need to drop into a large amount of funds.Therefore, find a kind of low cost truly, and crop immunity improved to effective immunizing agent, low-cost, without the immunizing agent of safety problem, be that people thirst for the problem solving always.
Summary of the invention
The technical problem to be solved in the present invention is that a kind of leaf vegetables immunizing agent and application thereof are provided.
Leaf vegetables immunizing agent of the present invention, its active component is comprised of the component of following weight portion: chitin and derivative 1～6.4 weight portion thereof, nitrogen 0.4～4 weight portion, phosphoric acid 0.3～1.0 weight portion, potassium chloride 0.8～5.0 weight portion; Wherein, described chitin and derivative thereof are water-soluble chitosan and derivative thereof; Nitrogen is Nitrogen element and the component that does not produce precipitation with water-soluble chitosan and derivatives reaction thereof, and quality is in nitrogen element quality.
Further, in order to improve the activity of leaf vegetables immunizing agent, except active component, in described leaf vegetables immunizing agent, also contain auxiliary material, described auxiliary material can adopt conventional auxiliary material, but considering cost and immunizing agent are active, described Optimization of Adjuvant alkyl glycoside and water, alkyl glycoside can be selected conventional type, and as APG0810, APG1214, APG0814, APG0816, APG1216 etc., addition is preferably 1～4 weight portion; Described water is preferably distilled water.
Further, effect crop immunity being promoted in order to improve leaf vegetables immunizing agent, described chitin and derivative thereof are at least one in shitosan, CMC, amino shitosan, deacetylated shitosan, deacetylated chitosan oligosaccharide; The preferred APG0810 of alkyl glycoside.
Further, as preferred version, its active component of leaf vegetables immunizing agent of the present invention is comprised of the component of following weight portion: chitin and derivative 3.2 weight portions thereof, urea 4.3 weight portions, phosphoric acid 0.9 weight portion, potassium chloride 2.1 weight portions, alkyl glycoside 2 weight portions; Wherein, described chitin and derivative thereof are deacetylated shitosan, and alkyl glycoside is APG0810.
Further, the invention still further relates to the preparation method of leaf vegetables immunizing agent, comprise the steps:
(1), according to proportioning, take each component;
(2), stir, dissolve, mix, obtain.
Further, the invention still further relates to the application of leaf vegetables immunizing agent in improving leaf vegetables crop immunity.Described leaf vegetables crop is common leaf vegetables crop, as celery, spinach, asparagus lettuce etc.
Further, in leaf vegetables immunizing agent, activity component concentration is too low can not bring into play its drought resisting effect, excessive concentration can exert an influence to crop, therefore, during application, preferably be diluted with water to chitin and derivative quality mark is 0.00067%～0.008%, be more preferably diluted to chitin and derivative quality mark is 0.00213%～0.004%.
Further, effect leaf vegetables crop immunity being promoted in order to improve leaf vegetables immunizing agent, described leaf vegetables immunizing agent is preferably applied to not occur the leaf vegetables crop of disease, and preferred vegetative growth phase of use age, more early result of use is better; More preferably adopt and be sprayed on plant blade face, further preferably evenly spray crop field top, with liquid cover completely plant surface not flow liquid be advisable.During use, preferably dilute 800-1500 and use after doubly, more preferably after 10-15 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times, amount of application is 0.04-0.122L/ mu.
Beneficial effect of the present invention:
1, each component of leaf vegetables immunizing agent of the present invention, all to human body and environmentally friendly, without complicated chemical composition, can not cause the residual of hormone yet, can not bring safety problem.
2, leaf vegetables immunizing agent of the present invention can well improve crop immunity, is better than commercially available prod.
3, in the present invention, leaf vegetables immunizing agent composition is simple, with low cost, prepares leaf vegetables immunizing agent of the present invention without dropping into large-scale special equipment.
4, in the present invention, leaf vegetables immunizing agent is easy to use, supplements crop alimentary when improving crop immunity, can reduce fertilising quantity.
Embodiment
Leaf vegetables immunizing agent of the present invention, its active component is comprised of the component of following weight portion: chitin and derivative 1～6.4 weight portion thereof, nitrogen 0.4～4 weight portion, phosphoric acid 0.3～1.0 weight portion, potassium chloride 0.8～5.0 weight portion; Wherein, described chitin and derivative thereof are water-soluble chitosan and derivative thereof; Nitrogen is Nitrogen element and the component that does not produce precipitation with water-soluble chitosan and derivatives reaction thereof, and quality is in nitrogen element quality.
Further, in order to improve the activity of leaf vegetables immunizing agent, except active component, in described leaf vegetables immunizing agent, also contain auxiliary material, described auxiliary material can adopt conventional auxiliary material, but considering cost and immunizing agent are active, described Optimization of Adjuvant alkyl glycoside and water, alkyl glycoside can be selected conventional type, and as APG0810, APG1214, APG0814, APG0816, APG1216 etc., addition is preferably 1～4 weight portion; Described water is preferably distilled water.
Further, effect crop immunity being promoted in order to improve leaf vegetables immunizing agent, described chitin and derivative thereof are at least one in shitosan, CMC, amino shitosan, deacetylated shitosan, deacetylated chitosan oligosaccharide; The preferred APG0810 of alkyl glycoside.
Further, as preferred version, its active component of leaf vegetables immunizing agent of the present invention is comprised of the component of following weight portion: chitin and derivative 3.2 weight portions thereof, urea 4.3 weight portions, phosphoric acid 0.9 weight portion, potassium chloride 2.1 weight portions, alkyl glycoside 2 weight portions; Wherein, described chitin and derivative thereof are deacetylated shitosan, and alkyl glycoside is APG0810.
Further, the invention still further relates to the preparation method of leaf vegetables immunizing agent, comprise the steps:
(1), according to proportioning, take each component;
(2), stir, dissolve, mix, obtain.
Further, the invention still further relates to the application of leaf vegetables immunizing agent in improving leaf vegetables crop immunity.Described leaf vegetables crop is common leaf vegetables crop, as celery, spinach, asparagus lettuce etc.
Further, in leaf vegetables immunizing agent, activity component concentration is too low can not bring into play its drought resisting effect, excessive concentration can exert an influence to crop, therefore, during application, preferably be diluted with water to chitin and derivative quality mark is 0.00067%～0.008%, be more preferably diluted to chitin and derivative quality mark is 0.00213%～0.004%.
Further, effect leaf vegetables crop immunity being promoted in order to improve leaf vegetables immunizing agent, described leaf vegetables immunizing agent is preferably applied to not occur the leaf vegetables crop of disease, and preferred vegetative growth phase of use age, more early result of use is better; More preferably adopt and be sprayed on plant blade face, further preferably evenly spray crop field top, with liquid cover completely plant surface not flow liquid be advisable.During use, preferably dilute 800-1500 and use after doubly, more preferably after 10-15 days, carry out secondary dispenser, the preferred continuous administration of a season crop 2～3 times, amount of application is 0.04-0.122L/ mu.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
The preparation of embodiment 1 leaf vegetables immunizing agent of the present invention
A, according to the corresponding content of each immunizing agent in table 1, weigh each component;
B, load weighted each component is dissolved respectively, finally stir, clear and get final product.
Table 1
Wherein, APG0810 is purchased from Shanghai Fakai Chemical Co., Ltd., lot number: 20120716.
Embodiment 2 leaf vegetables immunizing agent of the present invention performance test-celery
1, materials and methods
2.1 experiment material
Experiment crop: celery; The present embodiment adopts potted plant completing, and each processing has celery 6 basins;
Experimental pharmacy: MYJ-01, MYJ-02; Its formula is in Table 1.
2.2 experimental design
This tests 2 concentration gradients of organic immunizing agent design, contrasts as clear water.In whole process of the test, all processing community adopts same management method, and chemicals treatment situation is in Table 2.
Table 2
| Numbering | Medicament | Working concentration |
| MYJ-01-800× | MYJ-01 | 800 times |
| MYJ-01-1500× | MYJ-01 | 1500 times |
| MYJ-02-800× | MYJ-02 | 800 times |
| MYJ-02-1500× | MYJ-02 | 1500 times |
| CK | Clear water | —— |
2.3 experimental technique
In first day, with back of the body formula sprayer, the celery in seedling stage is carried out to dispenser, the present embodiment adopts the spot blight incidence of disease and the disease index of investigation statistics experiment crop; After 15 days, carry out data survey for the first time and carry out dispenser for the second time simultaneously, after 20 days, carry out data survey for the second time.
Embodiment of the present invention scab situation adopts following grade scale:
0 grade: without scab;
1 grade: lesion area accounts for whole leaf area below 5%;
3 grades: lesion area accounts for whole leaf area 6%～10%;
5 grades: lesion area accounts for whole leaf area 11%～20%;
7 grades: lesion area accounts for whole leaf area 21%～40%;
9 grades: lesion area accounts for whole leaf area more than 40%.
3 experimental results and analysis
3.1 experimental result
Original experimental data in Table 3, table 4, experimental result is in Table 5.
Table 3 is investigated state of an illness table of grading for the first time
Wherein, A represents the outermost leaf of celery plant; B represents second leaf of celery plant most external; C represents the 3rd leaf of celery plant most external, and A, B, C represent outermost three leaves of celery plant.
Table 4 is data survey state of an illness table of grading for the second time
Table 5 experimental result
Wherein, disease index and preventive effect effect computing formula are suc as formula shown in (1), (2):
Wherein, CK
0-blank district disease index before processing; CK
1-blank district disease index after processing; PT
0medicament treatment region disease index before-dispenser; PT
1chemicals treatment district disease index after-dispenser.
3.2 interpretation of result
As can be seen from Table 5, MYJ-01-800 * effect best, the drug effect duration is best, and MYJ-01-1500 * and MYJ-02-800 * initial stage control efficiency general, MYJ-02-1500 * control efficiency poor.Continuation and the spraying medicine concentration of drug effect have relation, concentration more the duration longer, the lower duration of concentration is shorter.
4 experiment conclusion
From experimental result, can find out, high concentration dispenser is better than low concentration, and leaf vegetables immunizing agent of the present invention has certain prevention effect to celery septoria disease, can improve to a certain extent the immunity of celery.
Embodiment 3 leaf vegetables immunizing agent of the present invention performance test-spinach
1, materials and methods
2.1 experiment material
Experiment crop: spinach; The present embodiment adopts potted plant completing, and each processing has spinach 5 basins;
Experimental pharmacy: MYJ-03, MYJ-04; Its formula is in Table 1.
2.2 experimental design
This tests 2 concentration gradients of organic immunizing agent design, contrasts as clear water.In whole process of the test, all processing community adopts same management method, and chemicals treatment situation is in Table 6.
Table 6
| Numbering | Medicament | Working concentration |
| MYJ-03-800× | MYJ-03 | 800 times |
| MYJ-03-1500× | MYJ-03 | 1500 times |
| MYJ-04-800× | MYJ-04 | 800 times |
| MYJ-04-1500× | MYJ-04 | 1500 times |
| CK | Clear water | —— |
2.3 experimental technique
In first day, with back of the body formula sprayer, the spinach in seedling stage is carried out to dispenser, the present embodiment adopts the Leucoplakia incidence of disease and the disease index of investigation statistics spinach; After 12 days, carry out data survey for the first time and carry out dispenser for the second time simultaneously, after 20 days, carry out data survey for the second time.
Embodiment of the present invention scab situation adopts following grade scale:
0 grade: without scab;
1 grade: lesion area accounts for whole leaf area below 5%;
3 grades: lesion area accounts for whole leaf area 6%～10%;
5 grades: lesion area accounts for whole leaf area 11%～20%;
7 grades: lesion area accounts for whole leaf area 21%～40%;
9 grades: lesion area accounts for whole leaf area more than 40%.
3 experimental results and analysis
3.1 experimental result
Original experimental data in Table 7, table 8, experimental result is in Table 9.
Table 7 is investigated state of an illness table of grading for the first time
Wherein, A represents the outermost leaf of spinach plant; B represents second leaf of spinach plant most external; C represents up the 3rd leaf of spinach plant bottom, and A, B, C represent the 3rd leaf of spinach plant most external.
8 investigate state of an illness table of grading for the second time
Table 9 experimental result
Wherein, disease index and preventive effect effect computing formula are suc as formula shown in (1), (2):
Wherein, CK
0-blank district disease index before processing; CK
1-blank district disease index after processing; PT
0medicament treatment region disease index before-dispenser; PT
1chemicals treatment district disease index after-dispenser.
As can be seen from Table 9, MYJ-03-800 * effect best, the drug effect duration is best, and MYJ-03-1500 * and MYJ-04-800 * control efficiency take second place, MYJ-04-1500 * control efficiency poor.Continuation and the spraying medicine concentration of drug effect have relation, concentration more the duration longer, the lower duration of concentration is shorter.
4 experiment conclusion
From experimental result, can find out, high concentration dispenser is better than low concentration, and leaf vegetables immunizing agent of the present invention has certain prevention effect to spinach Leucoplakia, can improve to a certain extent the immunity of spinach.
Embodiment 4 leaf vegetables immunizing agent of the present invention performance test-Chinese cabbage, asparagus lettuce etc.
Inventor takes the method for embodiment 2,3, adopt medicament MYJ-05, MYJ-06 respectively to dilute 800 and 1500 times the common leaf vegetables such as Chinese cabbage, asparagus lettuce have been carried out to same experiment, all find that leaf vegetables immunizing agent of the present invention can effectively prevent the Common Diseases of the leaf vegetables crops such as Chinese cabbage, asparagus lettuce, for improving leaf vegetables crop immunity, have a certain upgrade.
Claims (10)
1. leaf vegetables immunizing agent, is characterized in that: its active component is comprised of the component of following weight portion: chitin and derivative 1～6.4 weight portion thereof, nitrogen 0.4～4 weight portion, phosphoric acid 0.3～1.0 weight portion, potassium chloride 0.8～5.0 weight portion; Wherein, described chitin and derivative thereof are water-soluble chitosan and derivative thereof; Nitrogen is Nitrogen element and the component that does not produce precipitation with water-soluble chitosan and derivatives reaction thereof, and quality is in nitrogen element quality.
2. leaf vegetables immunizing agent according to claim 1, is characterized in that: except active component, in described leaf vegetables immunizing agent, also contain auxiliary material, described auxiliary material is alkyl glycoside and water.
3. leaf vegetables immunizing agent according to claim 1 and 2, is characterized in that: described chitin and derivative thereof are at least one in shitosan, CMC, amino shitosan, deacetylated shitosan, deacetylated chitosan oligosaccharide; Alkyl glycoside is APG0810.
4. leaf vegetables immunizing agent according to claim 3, it is characterized in that: its active component is comprised of the component of following weight portion: chitin and derivative 3.2 weight portions thereof, urea 4.3 weight portions, phosphoric acid 0.9 weight portion, potassium chloride 2.1 weight portions, alkyl glycoside 2 weight portions; Wherein, described chitin and derivative thereof are deacetylated shitosan, and alkyl glycoside is APG0810.
5. the application of the leaf vegetables immunizing agent described in claim 1～4 any one in improving leaf vegetables crop immunity.
6. the application of leaf vegetables immunizing agent according to claim 5 in improving leaf vegetables crop immunity, is characterized in that: described leaf vegetables crop is celery, spinach, asparagus lettuce.
7. the application of leaf vegetables immunizing agent according to claim 6 in improving leaf vegetables crop immunity, is characterized in that: during application, be diluted with water to chitin and derivative quality mark is 0.00067%～0.008%.
8. the application of leaf vegetables immunizing agent according to claim 7 in improving leaf vegetables crop immunity, is characterized in that: during application, be diluted with water to chitin and derivative quality mark is 0.00213%～0.004%.
9. the application in improving leaf vegetables crop immunity according to the leaf vegetables immunizing agent described in claim 5～8 any one, is characterized in that: described leaf vegetables immunizing agent is applied to not occur the leaf vegetables crop of disease, the use age is crop alimentary vegetative period.
10. the application in improving leaf vegetables crop immunity according to the leaf vegetables immunizing agent described in claim 5～9 any one, is characterized in that: described in be applied as and spray in crop field top, make it cover plant surface and flow liquid not completely.
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| CN201310554240.1A CN103548878B (en) | 2013-11-08 | 2013-11-08 | Leaf-vegetable immunizing agent and application thereof |
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| CN103548878B CN103548878B (en) | 2015-04-15 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104926478A (en) * | 2015-06-11 | 2015-09-23 | 湖北华丰生物化工有限公司 | Slow/controlled-release compound organic fertilizer and preparation method thereof |
| CN106064984A (en) * | 2016-05-31 | 2016-11-02 | 钟祥尤生农药有限公司 | A kind of soil improvement immunizing agent and its preparation method and application |
| CN110105133A (en) * | 2019-04-28 | 2019-08-09 | 湖北工程学院 | A kind of dedicated degeneration-resistant foliar fertilizer of Brassica rapa L and its application method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432378A (en) * | 2011-09-19 | 2012-05-02 | 北京雷力农用化学有限公司 | Foliar fertilizer and preparation method thereof |
| CN103204729A (en) * | 2012-01-17 | 2013-07-17 | 山东新沭化化肥有限公司 | Composite fertilizer comprising chitin, humic acid, and middle and trace elements |
-
2013
- 2013-11-08 CN CN201310554240.1A patent/CN103548878B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432378A (en) * | 2011-09-19 | 2012-05-02 | 北京雷力农用化学有限公司 | Foliar fertilizer and preparation method thereof |
| CN103204729A (en) * | 2012-01-17 | 2013-07-17 | 山东新沭化化肥有限公司 | Composite fertilizer comprising chitin, humic acid, and middle and trace elements |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104926478A (en) * | 2015-06-11 | 2015-09-23 | 湖北华丰生物化工有限公司 | Slow/controlled-release compound organic fertilizer and preparation method thereof |
| CN106064984A (en) * | 2016-05-31 | 2016-11-02 | 钟祥尤生农药有限公司 | A kind of soil improvement immunizing agent and its preparation method and application |
| CN110105133A (en) * | 2019-04-28 | 2019-08-09 | 湖北工程学院 | A kind of dedicated degeneration-resistant foliar fertilizer of Brassica rapa L and its application method |
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| Publication number | Publication date |
|---|---|
| CN103548878B (en) | 2015-04-15 |
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Address after: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee after: Chengdu new Chaoyang Crop Science Co., Ltd Address before: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Patentee before: CHENGDU NEWSUN CROPSCIENCE Co.,Ltd. |