CN104336548A - Sea buckthorn fruit probiotics powder and preparation method thereof - Google Patents
Sea buckthorn fruit probiotics powder and preparation method thereof Download PDFInfo
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- CN104336548A CN104336548A CN201410212581.5A CN201410212581A CN104336548A CN 104336548 A CN104336548 A CN 104336548A CN 201410212581 A CN201410212581 A CN 201410212581A CN 104336548 A CN104336548 A CN 104336548A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
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Abstract
The invention provides a sea buckthorn fruit probiotics powder and a preparation method thereof. Crushed dry sea buckthorn fruits or press dregs of fresh sea buckthorn fruits are placed into a glass percolator and then extracted for many times with 70% ethanol, >=85% ethanol and hexane solvent respectively, extracts are combined; raw milk is pasteurized, probiotics in an amount of 5% of the weight of the raw milk is added into the raw milk and the milk ferments for more than 72 hours at 40-42 DEG C in a yogurt maker, the milk is frozen for 12 hours at the pH of 3.5, and probiotics ferment is obtained, wherein the probiotics are formed by mixing Lactobacillus acidophilus and Bifidobacterium longum according to the ratio of 1:1. The extract is mixed with the probiotics ferment, the mixture is separated in a centrifuge, a filter cake is obtained and then processed by a vacuum freeze drier, and thus the sea buckthorn fruit probiotics powder is obtained. The sea buckthorn fruit probiotics powder can be taken daily with normal temperature water before bedtime every night, with the dosage of 2.5 g for each time.
Description
Technical field
The present invention relates to the production of probiotic powder, while the method obtains the Dietotherapy health product of high-quality from fructus hippophae, for consumer provides the theory of family workshop type, be worth using for reference.
Background technology
The composition of fructus hippophae not only enriches, and comprehensively, the more important thing is that these biochemical components have mutually collaborative, through modern science experiment and clinical practice, the function of mutual synergy, proves that sea-buckthorn has multiple dietotherapy and health care.
The fructus hippophae that the present invention selects contains multiple biochemical component: (1) multiple flavone compound; (2) grease and fatty acid; (3) phenols and organic acid; Various trace elements potassium, sodium, calcium, magnesium, zinc, iron, selenium, copper, manganese equal size is very high matches with human body requirements; (5) triterpene, steroid compound; (6) oil & fat acids; (7) volatile oil; (8) protein and amino acids; (9) phospholipid.
The present invention selects the water ease of solubility of probio ferment (ox yogurt), slightly dissolubility, microsolubility and insoluble biochemical component: be mainly present in stomach and small intestine addicted to the dry bacterium of yogurt, release digestive ferment, help food digestion, protection gastrointestinal wall, bone growth and health of heart are played an important role; Bifidobacterium longum is mainly present in large intestine, in reproductive process, sugar fermentation produces a large amount of lactic acid and acetic acid, acidificable enteron aisle, suppress pathogenic bacteria growth, the illnesss such as diarrhoea, constipation can be prevented and treated, the acidic metabolite produced, can promote the absorption of iron, VD and calcium, plays an important role to bone growth and health of heart.
Sea-buckthorn of the present invention
reallyprobiotic powder is by the active ingredient of fructus hippophae and probio ferment biological active component efficient combination, produce mutually collaborative to the nutritional labeling of healthy human body, mutual synergistic function, the preparation method of product is simple, simultaneous adaptation family workshop, puts goods on the market and must be subject to the favor of consumer.
Summary of the invention
The object of the invention is to: the product of the fructus hippophae probio provided, the nutritional labeling of healthy human body is produced mutually collaborative, mutual synergistic function.
The object of the present invention is achieved like this: a kind of fructus hippophae probiotic powder and preparation method thereof, implements step by step;
Step 1 sea-buckthorn dry fruit coarse grain extracts:
Sea-buckthorn dry fruit is placed in pulverizer pulverize, obtains the coarse grain that aperture is 850 ± 29 microns, for subsequent use;
1) by meal 84-120g, be placed in glass percolator, adopt 70% ethanol extraction, first time adds 780-820ml, normal temperature, lixiviate 72h, second time adds 690-730ml lixiviate 48h, third time adds 580-650ml lixiviate 24h, coarse filtration liquid sedimentation 24h, smart filtrate recycling ethanol, water bath method must extract slag, dry, continue under walk extraction;
2) step extraction slag 82-90g is got, adopt >=85% ethanol extraction, first time adds 770-830ml, normal temperature, lixiviate 72h, second time adds 680-740ml, lixiviate 48h, third time adds 570-640ml lixiviate 24h, coarse filtration liquid sedimentation 24h, essence filtrate recycling ethanol, water bath method obtains extract, dries, and walks extraction under continuation;
3) get step extraction slag 78-82g, adopt n-hexane extraction, first time adds 580-620ml, normal temperature, lixiviate 72h, second time 480-530ml lixiviate 48h, third time adds 380-440ml lixiviate 24h, coarse filtration liquid sedimentation 24h, smart filtrate reclaims n-hexane, and water bath method must extract slag; Wherein solvent residual amount≤25ppm;
By 1), 2), 3) sea-buckthorn dry fruit extract merge use;
The extraction of step 2 sea-buchthorn fresh fruit:
Sea-buchthorn fresh fruit is refrigerated to subzero, adopts running water and deionization to clean, thaw, squeeze, filter to obtain filter residue, for subsequent use;
1) by fresh fruit filter residue 30-35g, be placed in glass percolator, adopt 70% ethanol extraction, first time adds 270-330ml, normal temperature, lixiviate 72h, second time adds 230-275ml lixiviate 48h, and third time adds 175-225ml lixiviate 24h, coarse filtration liquid sedimentation 24h, essence filtrate recycling ethanol, water bath method must extract slag, dries, and walks extraction under continuation;
2) step extraction slag 16-20g is got, adopt >=85% ethanol extraction, first time adds 230-270ml, normal temperature, lixiviate 72h, and second time adds 180-230ml lixiviate 48h, third time adds 175-215ml lixiviate 24h, coarse filtration liquid, sedimentation 24h, smart filtrate recycling ethanol, water bath method must extract slag, walks extraction under drying continuation;
3) step extraction slag 13-16g is got, adopt n-hexane extraction, first time adds 85-120ml, normal temperature, lixiviate 72h, and second time adds 80-110ml lixiviate 48h, third time adds 75-100ml lixiviate 24h, coarse filtration liquid sedimentation 24h, smart filtrate reclaims n-hexane, and water bath method must extract slag, dry, for subsequent use, wherein solvent residual amount≤25ppm;
By 1), 2), 3) sea-buchthorn fresh fruit extract merge use;
Step 3 produces probio ferment
By raw milk through pasteurization, add the 5% probio bacterial classification accounting for raw milk weight, be placed in Yogurt making machine fermentation, temperature 40-42 DEG C, time more than 72h, at refrigeration 12h, pH value is 3.5, obtains probio ferment, and wherein probio bacterial classification is mixed by lactobacillus acidophilus and bifidobacterium longum 1:1 and be get final product;
Step 4 fructus hippophae probiotic powder is produced
Sea-buckthorn dry fruit extraction mixture or sea-buchthorn fresh fruit extraction mixture are mixed with probio ferment, stirring stirs evenly, and obtains extract probio ferment, the ratio > 80% wherein shared by probio ferment; Extract probio ferment is placed in centrifuge to be separated, filter cake is disposed through vacuum freeze drier, obtains fructus hippophae probiotic powder, pack, every bag of 2.5g.
Mechanism of the present invention and effect: the wild sea buckthorn resources in Xinjiang accounts for the whole nation more than 90%, efficent use of resources alleviation production autumn and winter is busy, spring and summer stops production phenomenon, adopt and wildly plant fructus hippophae probiotic powder prepared by the method that develops simultaneously with domestication and contain fructus hippophae and probio ferment major part bioactive ingredients, containing a large amount of nutritional labelings that human body needs, take simultaneously, these biochemical components have mutually collaborative, mutual synergistic function, develop immunitypty, make health more healthy, show technological progress.
Detailed description of the invention
The present invention is further described in conjunction with the embodiments.
Embodiment
One, mixed extracts is produced
1, wild sea-buckthorn dry fruit is placed in pulverizer to pulverize, obtains the coarse grain that aperture is 850 ± 29 microns, for subsequent use;
1) 70% ethanol extraction water omits the biochemical components such as dissolubility flavonoid glycoside
Get sea-buckthorn dry fruit coarse grain 100g, be placed in glass percolator, use 70% ethanol extraction, first time adds 800ml, normal temperature, lixiviate 72h, second time adds 700ml lixiviate 48h, and third time adds 600ml lixiviate 24h, coarse filtration liquid, sedimentation 24h, the filter of fine canvas essence, smart filtrate recycling ethanol, water bath method obtains extract, and extraction slag dries for subsequent use.
2) >=85% ethanol extraction water microsolubility Hippophate flavone glucoside unit waits biochemical component
Get step 1) extraction slag 87.8g, adopt >=85% ethanol extraction, first time adds 800ml normal temperature, lixiviate 72h, and second time adds 700ml lixiviate 48h, and third time adds 600ml lixiviate 24h, coarse filtration liquid, sedimentation 24h, the filter of fine canvas essence, smart filtrate recycling ethanol, water bath method obtains extract, and extraction slag dries for subsequent use.
3) n-hexane extraction water-insoluble seabuckthorn seeds wet goods biochemical component
Get step 2) extraction slag 82g, adopt n-hexane extraction, first time adds 600ml normal temperature, lixiviate 72h, and second time adds 500ml lixiviate 48h, third time adds 400ml lixiviate 24h, coarse filtration liquid, sedimentation 24h, the filter of fine canvas essence, essence filtrate reclaims n-hexane, water bath method obtains extract, and wherein n-hexane adopts vacuum decompression evaporation to drive away, solvent residual amount≤25ppm.
By 1), 2), 3) extract merge use.
Or 1) get meal 82g, be placed in glass percolator, adopt 70% ethanol extraction, first time adds 780ml, normal temperature, lixiviate 72h, second time adds 690ml lixiviate 48h, third time adds 580ml lixiviate 24h, coarse filtration liquid sedimentation 24h, smart filtrate recycling ethanol, water bath method must extract slag, dry, continue under walk extraction;
2) get step extraction slag 80g, adopt >=85% ethanol extraction, first time adds 770ml, normal temperature, lixiviate 72h, second time adds 680ml, lixiviate 48h, and third time adds 570ml lixiviate 24h, coarse filtration liquid sedimentation 24h, essence filtrate recycling ethanol, water bath method obtains extract, dries, and walks extraction under continuation;
3) get step extraction slag 78g, adopt n-hexane extraction, first time adds 580ml, normal temperature, lixiviate 72h, second time 480ml lixiviate 48h, and third time adds 380ml lixiviate 24h, coarse filtration liquid sedimentation 24h, and smart filtrate reclaims n-hexane, and water bath method must extract slag; Wherein solvent residual amount≤25ppm;
By 1), 2), 3) sea-buckthorn dry fruit extract merge use;
2, get cultivation sea-buchthorn fresh fruit freezing subzero, running water and deionized water are cleaned, thaw, squeeze, coarse filtration, calico are smart filters, obtain filter residue and dry, for subsequent use.
1) 70% ethanol extraction water omits the biochemical components such as dissolubility flavonoid glycoside
Get fresh fruit filter residue powder 33,8g, be placed in glass percolator, adopt 70% ethanol extraction, first time adds 300ml, normal temperature, lixiviate 72h, second time adds 250ml lixiviate 48h, and third time adds 200ml lixiviate 24h, coarse filtration liquid, sedimentation 24h, the filter of fine canvas essence, smart filtrate recycling ethanol, water bath method obtains extract 13.1g, and extraction slag dries for subsequent use.
2) >=85% ethanol extraction water microsolubility Hippophate flavone glucoside unit waits biochemical component
Get step 1) extraction slag 18.5g, adopt >=85% ethanol extraction, first time add 250ml, normal temperature, lixiviate 72h, second time adds 200ml lixiviate 48h, and third time adds 195ml lixiviate 24h, coarse filtration liquid, sedimentation 24h, the filter of fine canvas essence, smart filtrate recycling ethanol, the extract of water bath method, extraction slag dries for subsequent use.
3) n-hexane extraction water-insoluble seabuckthorn seeds wet goods biochemical component
Get step 2) extraction slag 15.5g, adopt n-hexane extraction, first time add 100ml, normal temperature lixiviate 72h, second time adds 100ml lixiviate 48h, and third time adds 100ml lixiviate 24h, coarse filtration liquid, sedimentation 24h, the filter of fine canvas essence, smart filtrate reclaims n-hexane, and water bath method obtains extract 1.5g; Wherein n-hexane adopts vacuum decompression evaporation to drive away, solvent residual amount≤25ppm.
By 1), 2), 3) extract merge use.
Or 1) get fresh fruit filter residue 35g, be placed in glass percolator, adopt 70% ethanol extraction, first time adds 330ml, normal temperature, lixiviate 72h, second time adds 275ml lixiviate 48h, and third time adds 225ml lixiviate 24h, coarse filtration liquid sedimentation 24h, essence filtrate recycling ethanol, water bath method must extract slag, dries, and walks extraction under continuation;
2) get step extraction slag 20g, adopt >=85% ethanol extraction, first time adds 270ml, normal temperature, lixiviate 72h, second time adds 230ml lixiviate 48h, and third time adds 215ml lixiviate 24h, coarse filtration liquid, sedimentation 24h, essence filtrate recycling ethanol, water bath method must extract slag, walks extraction under drying continuation;
3) step extraction slag 16g is got, adopt n-hexane extraction, first time adds 120ml, normal temperature, lixiviate 72h, and second time adds 110ml lixiviate 48h, third time adds 100ml lixiviate 24h, coarse filtration liquid sedimentation 24h, smart filtrate reclaims n-hexane, and water bath method must extract slag, dry, for subsequent use, wherein solvent residual amount≤25ppm;
By 1), 2), 3) sea-buchthorn fresh fruit extract merge use;
Two, probio ferment (ox yogurt) is produced
Raw milk (market purchasing), through pasteurization, adds 5% probiotics leaven accounting for raw milk gross weight, with Yogurt making machine fermentation, and temperature 40-42 DEG C, fermentation more than 72h, after refrigeration 12h, pH value is 3.5, obtains probio ferment; Wherein probio bacterial classification is that lactobacillus acidophilus and bifidobacterium longum 1:1 mix obtained (lactobacillus acidophilus and bifidobacterium longum are purchased from Beijing Borrow's biological products Co., Ltd).
Three, the producing of sea-buckthorn dry fruit (or fructus hippophae fresh fruit) extract probio ferment
1) sea-buckthorn dry fruit extract probio ferment is produced
Get sea-buckthorn dry fruit extract, namely 1), 2), 3) extraction consolidated material mix (wherein raw milk content remains on more than 80%) with probio ferment and stir, obtain sea-buckthorn dry fruit extract probio ferment.
2) sea-buchthorn fresh fruit slag extract probio ferment is produced
Sea-buchthorn fresh fruit slag extract, namely 1), 2), 3) extraction consolidated material mix (wherein raw milk content remains on more than 80%) with probio ferment and stir, obtain fructus hippophae extract probio ferment.
Four, fructus hippophae probiotic powder is produced
Get sea-buckthorn dry fruit extract probio ferment or sea-buchthorn fresh fruit slag extract probio ferment to be placed in centrifuge and to be separated, by filter cake, be placed in vacuum freeze drying, obtain fructus hippophae probiotic powder, packaging of aluminium foil bag, 2.5g/ bag, 10 bags/box.
This product consumption: normal-temperature water takes, evening every day just before going to bed, takes 1 bag.
This product storage practice: shady and cool dry place, or stored refrigerated effect is better; The term of validity 18 months, this product can not alternatives to medication.
Five, probio quantitative measurement: every 100g fructus hippophae probiotic powder should meet B19302-2010 " acidified milk " lactic acid bacteria number: >=1 × 106cfu/g (ml) containing lactobacillus acidophilus and bifidobacterium longum quantity; Wherein fermented bacterium, lactobacillus bulgaricus, streptococcus thermophilus or other bacterial classification used by the approval of administrative department of State Council.
Claims (2)
1. fructus hippophae probiotic powder and preparation method thereof, is characterized in that: implement step by step;
Step 1 sea-buckthorn dry fruit coarse grain extracts:
Sea-buckthorn dry fruit is placed in pulverizer pulverize, obtains the coarse grain that aperture is 850 ± 29 microns, for subsequent use;
1) by meal 84-120g, be placed in glass percolator, adopt 70% ethanol extraction, first time adds 780-820ml, normal temperature, lixiviate 72h, second time adds 690-730ml lixiviate 48h, third time adds 580-650ml lixiviate 24h, coarse filtration liquid sedimentation 24h, smart filtrate recycling ethanol, water bath method must extract slag, dry, continue under walk extraction;
2) step extraction slag 82-90g is got, adopt >=85% ethanol extraction, first time adds 770-830ml, normal temperature, lixiviate 72h, second time adds 680-740ml, lixiviate 48h, third time adds 570-640ml lixiviate 24h, coarse filtration liquid sedimentation 24h, essence filtrate recycling ethanol, water bath method obtains extract, dries, and walks extraction under continuation;
3) get step extraction slag 78-82g, adopt n-hexane extraction, first time adds 580-620ml, normal temperature, lixiviate 72h, second time 480-530ml lixiviate 48h, third time adds 380-440ml lixiviate 24h, coarse filtration liquid sedimentation 24h, smart filtrate reclaims n-hexane, and water bath method must extract slag; Wherein solvent residual amount≤25ppm;
By 1), 2), 3) sea-buckthorn dry fruit extract merge use;
The extraction of step 2 sea-buchthorn fresh fruit slag:
Sea-buchthorn fresh fruit is refrigerated to subzero, adopts running water and deionization to clean, thaw, squeeze, filter to obtain filter residue, for subsequent use;
1) by fresh fruit filter residue 30-35g, be placed in glass percolator, adopt 70% ethanol extraction, first time adds 270-330ml, normal temperature, lixiviate 72h, second time adds 230-275ml lixiviate 48h, and third time adds 175-225ml lixiviate 24h, coarse filtration liquid sedimentation 24h, essence filtrate recycling ethanol, water bath method must extract slag, dries, and walks extraction under continuation;
2) step extraction slag 16-20g is got, adopt >=85% ethanol extraction, first time adds 230-270ml, normal temperature, lixiviate 72h, and second time adds 180-230ml lixiviate 48h, third time adds 175-215ml lixiviate 24h, coarse filtration liquid, sedimentation 24h, smart filtrate recycling ethanol, water bath method must extract slag, walks extraction under drying continuation;
3) step extraction slag 13-16g is got, adopt n-hexane extraction, first time adds 85-120ml, normal temperature, lixiviate 72h, and second time adds 80-110ml lixiviate 48h, third time adds 75-100ml lixiviate 24h, coarse filtration liquid sedimentation 24h, smart filtrate reclaims n-hexane, and water bath method must extract slag, dry, for subsequent use, wherein solvent residual amount≤25ppm;
By 1), 2), 3) sea-buchthorn fresh fruit extract merge use;
Step 3 produces probio ferment
By raw milk through pasteurization, add the 5% probio bacterial classification accounting for raw milk weight, be placed in Yogurt making machine fermentation, temperature 40-42 DEG C, time more than 72h, at refrigeration 12h, pH value is 3.5, obtains probio ferment, and wherein probio bacterial classification is mixed by lactobacillus acidophilus and bifidobacterium longum 1:1 and be get final product;
Step 4 fructus hippophae probiotic powder is produced
Sea-buckthorn dry fruit extraction mixture or sea-buchthorn fresh fruit extraction mixture are mixed with probio ferment, stirring stirs evenly, and obtains extract probio ferment, the ratio > 80% wherein shared by probio ferment; Extract probio ferment is placed in centrifuge to be separated, filter cake is disposed through vacuum freeze drier, obtains fructus hippophae probiotic powder, pack, every bag of 2.5g.
2., according to method described in claim 1, it is characterized in that: raw milk, probio microbial inoculum are market purchasing.
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| CN104940450A (en) * | 2015-05-22 | 2015-09-30 | 北京联华大通教育科技有限公司 | Health beverage for enhancing body immunity and preparation method thereof |
| CN106306812A (en) * | 2015-06-17 | 2017-01-11 | 合生元（广州）健康产品有限公司 | Fruit probiotic fermentation preparation method |
| CN106418545A (en) * | 2016-09-09 | 2017-02-22 | 杨森健 | Functional food with balanced nutrients |
| CN108030096A (en) * | 2017-12-18 | 2018-05-15 | 浙江民生健康科技有限公司 | A kind of calcium iron zinc and probiotic composition and its application |
| CN108208793A (en) * | 2017-12-18 | 2018-06-29 | 山西金海利生物科技有限公司 | A kind of preparation method of fermentation hippophae rhamnoides compositions for improving intestine immunity |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106306812A (en) * | 2015-06-17 | 2017-01-11 | 合生元（广州）健康产品有限公司 | Fruit probiotic fermentation preparation method |
| CN106418545A (en) * | 2016-09-09 | 2017-02-22 | 杨森健 | Functional food with balanced nutrients |
| CN108030096A (en) * | 2017-12-18 | 2018-05-15 | 浙江民生健康科技有限公司 | A kind of calcium iron zinc and probiotic composition and its application |
| CN108208793A (en) * | 2017-12-18 | 2018-06-29 | 山西金海利生物科技有限公司 | A kind of preparation method of fermentation hippophae rhamnoides compositions for improving intestine immunity |
| CN108208793B (en) * | 2017-12-18 | 2021-05-04 | 山西金海利生物科技有限公司 | Preparation method of fermented sea buckthorn composition capable of improving intestinal immunity |
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Application publication date: 20150211 |