CN104330326A - Measuring method of nitrogen content in soil - Google Patents
Measuring method of nitrogen content in soil Download PDFInfo
- Publication number
- CN104330326A CN104330326A CN201410585730.2A CN201410585730A CN104330326A CN 104330326 A CN104330326 A CN 104330326A CN 201410585730 A CN201410585730 A CN 201410585730A CN 104330326 A CN104330326 A CN 104330326A
- Authority
- CN
- China
- Prior art keywords
- nitrogen
- soil
- nitrogen content
- nitrate
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention provides a measuring method of nitrogen content in soil. The measuring method comprises the following steps: (1) drying soil samples, then weighing 1 g of soil samples to be put into a 100 ml Kjeldahl bottle, and removing interfering substances; (2) oxidizing nitrite nitrogen into nitrate nitrogen in the samples of which the interfering substances are removed; (3) reducing the nitrate nitrogen in the samples into ammonium nitrogen; (4) absorbing distilled ammonia with boracic acid after alkalization, and titrating with a standard acid solution, so as to determine the total nitrogen content in the soil, wherein N%=[(V-V0)*N*0.014]/sample weight*100%. According to the measuring method, the detection result of the nitrogen content in the soil is accurate, the interfering substances are removed, the measuring method is convenient for knowing about the total nitrogen content in the soil, and the detection result can be used as reference for fertilization, so as to guide fertilization to achieve a yield-increasing effect.
Description
technical field:
The present invention relates to a kind of assay method of nitrogen content in soil, belong to environmental monitoring technology field.
background technology:
Soil is the main source of crop nitrogen nutrition, and the nitrogen in soil comprises inorganic nitrogen and the large class of organic nitrogen two, and wherein more than 95% is organic nitrogen, mainly comprises soil ulmin, protein, amino acid etc.Micromolecular amino acid can directly be absorbed by plants, and organic nitrogen has to pass through mineralization and is converted into ammonium, could be absorbed by crops, belongs to slow nitrogen.
In total soil nitrogen, inorganic nitrogen content is less than 5%, mainly ammonium and nitrate, and nitrite, ammonia, nitrogen and oxides of nitrogen etc. are little.Major part ammonium nitrogen and nitrate nitrogen are easily directly absorbed by crop, belong to available nitrogen.Inorganic nitrogen comprises the nitrate radical be present in the soil liquid and the ammonium ion be adsorbed on soil particle, and crop can directly absorb.Soil is very weak to the absorption of nitrate radical, so nitrate radical is very easy to outflow with water.Under the reducing conditions, nitrate radical can be reduced to gaseous nitrogen and soil of overflowing under the effect of microorganism, i.e. denitrification denitrogenation.Part ammonium ion can be fixed by clay mineral and be difficult to absorbed by crops, and is very easy in alkaline soils vapor away with the form of ammonia.In the building-up process of agron, also can utilize a large amount of inorganic nitrogen, because soil ulmin decomposes very slow, the validity of these nitrogens is very low.Nitrogen in soil is mainly from fertilising, biological nitrogen fixation, rainwater and irrigation water, and the latter two are very little to Soil Nitrogen contribution, and fertilising is the main source of anthropogenic soil nitrogen, and the nitrogen of natural soils is mainly from biological nitrogen fixation.
Soil nitrogenous amount affects by vegetation, temperature, farming, fertilising etc., and top layer, general arable land nitrogen content is 0.05% ~ 0.30%, and the topsoil nitrogen content in the arable land that minority is fertile, grassland, forest land is more than 0.50% ~ 0.60%.The nitrogen content of China's soil, reduces from east to west, from north orientation south gradually.No matter enter the nitrogen of the various forms in soil, be chemical fertilizer, or organic fertilizer, mutually can transform under the effect of physics, chemistry and biodyne.
Soil nitrogenous amount number and existence, output that is normal and crop has certain positive correlation under a certain condition, and from current China soil fertility, the soil of about 80% all lacks nitrogen.Therefore, understand total soil nitrogen amount, can be used as the reference of fertilising, to instruct fertilising to reach effect of increasing production.
summary of the invention:
The object of the invention is the assay method that a kind of nitrogen content in soil is provided for above-mentioned Problems existing, be convenient to understand total soil nitrogen amount, can be used as the reference of fertilising, to instruct fertilising to reach effect of increasing production.
Above-mentioned object is achieved through the following technical solutions:
The assay method of nitrogen content in soil, the method comprises the steps:
(1) taking 1g after pedotheque being carried out drying is put in 100ml kelvin bottle, removes interfering material;
(2) nitrite nitrogen in the sample after removal interfering material is oxidized to nitrate nitrogen;
(3) by the nitrate-nitrogen reduction in sample, ammonium nitrogen is converted into;
(4) the ammonia boric acid distilled after alkalization absorbs, and with sour standard solution titration, obtains total nitrogen content of soil, N%=[(V-V0) × N × 0.014]/sample heavy × 100%,
In formula:
The milliliter number of quota of expenditure hydrochloric acid during V-titration;
The milliliter number of quota of expenditure hydrochloric acid when V0-titration is blank;
The volumetric molar concentration of N-standard hydrochloric acid;
The mM quality g/mmol of 0.014-nitrogen-atoms;
The assay method of described nitrogen content in soil, the method of the removal interfering material described in step (1) is: in pedotheque, add the concentrated sulphuric acid 6-8ml that mass concentration is 98%, after stirring, heating, makes sulfuric acid smolder, exists until can't see black carbon granules.
The assay method of described nitrogen content in soil, the method that the nitrite nitrogen described in step (2) is oxidized to nitrate nitrogen is: add the liquor potassic permanganate that 6-8g mass percentage concentration is 5% in the sample to which, and after stirring, 95-100 DEG C is heated 10 minutes.
The assay method of described nitrogen content in soil, the reductive agent nitrate-nitrogen reduction in sample adopted described in step (3) is iron powder.
Beneficial effect:
The present invention is accurate to the nitrogen content testing result in soil, eliminates the interference of material, is convenient to understand total soil nitrogen amount, can be used as the reference of fertilising, to instruct fertilising to reach effect of increasing production.
embodiment:
Embodiment 1:
The assay method of nitrogen content in soil, the method comprises the steps:
(1) take 1g after pedotheque being carried out drying to be put in 100ml kelvin bottle, add the concentrated sulphuric acid 6-8ml that mass concentration is 98% in pedotheque, after stirring, heating, makes sulfuric acid smolder, and exists until can't see black carbon granules;
(2) add the liquor potassic permanganate that 6-8g mass percentage concentration is 5% in the sample to which, after stirring, 95-100 DEG C is heated 10 minutes, and the nitrite nitrogen in the sample after removal interfering material is oxidized to nitrate nitrogen;
(3) adopt reduced iron powder by the nitrate-nitrogen reduction in sample, be converted into ammonium nitrogen;
(4) the ammonia boric acid distilled after alkalization absorbs, and with sour standard solution titration, obtains total nitrogen content of soil, N%=[(V-V0) × N × 0.014]/sample heavy × 100%,
In formula:
The milliliter number of quota of expenditure hydrochloric acid during V-titration;
The milliliter number of quota of expenditure hydrochloric acid when V0-titration is blank;
The volumetric molar concentration of N-standard hydrochloric acid;
The mM quality g/mmol of 0.014-nitrogen-atoms;
Below be only most preferred embodiment of the present invention, method of the present invention includes but not limited to above-described embodiment, and unaccomplished matter of the present invention belongs to the common practise of those skilled in the art.
Claims (4)
1. an assay method for nitrogen content in soil, is characterized in that: the method comprises the steps:
(1) taking 1g after pedotheque being carried out drying is put in 100ml kelvin bottle, removes interfering material;
(2) nitrite nitrogen in the sample after removal interfering material is oxidized to nitrate nitrogen;
(3) by the nitrate-nitrogen reduction in sample, ammonium nitrogen is converted into;
(4) the ammonia boric acid distilled after alkalization absorbs, and with sour standard solution titration, obtains total nitrogen content of soil, N%=[(V-V0) × N × 0.014]/sample heavy × 100%,
In formula:
The milliliter number of quota of expenditure hydrochloric acid during V-titration;
The milliliter number of quota of expenditure hydrochloric acid when V0-titration is blank;
The volumetric molar concentration of N-standard hydrochloric acid;
The mM quality g/mmol of 0.014-nitrogen-atoms.
2. the assay method of nitrogen content in soil according to claim 1, it is characterized in that: the method for the removal interfering material described in step (1) is: in pedotheque, add the concentrated sulphuric acid 6-8ml that mass concentration is 98%, heating after stirring, sulfuric acid is smoldered, exists until can't see black carbon granules.
3. the assay method of nitrogen content in soil according to claim 1, it is characterized in that: the method that the nitrite nitrogen described in step (2) is oxidized to nitrate nitrogen is: add the liquor potassic permanganate that 6-8g mass percentage concentration is 5% in the sample to which, after stirring, 95-100 DEG C is heated 10 minutes.
4. the assay method of nitrogen content in soil according to claim 1, is characterized in that: the reductive agent nitrate-nitrogen reduction in sample adopted described in step (3) is iron powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410585730.2A CN104330326A (en) | 2014-10-28 | 2014-10-28 | Measuring method of nitrogen content in soil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410585730.2A CN104330326A (en) | 2014-10-28 | 2014-10-28 | Measuring method of nitrogen content in soil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104330326A true CN104330326A (en) | 2015-02-04 |
Family
ID=52405096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410585730.2A Pending CN104330326A (en) | 2014-10-28 | 2014-10-28 | Measuring method of nitrogen content in soil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104330326A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424871A (en) * | 2015-12-10 | 2016-03-23 | 苏州国环环境检测有限公司 | Determination method for ammonia nitrogen in wastewater |
| CN105527237A (en) * | 2015-12-16 | 2016-04-27 | 河南大学 | Method for determining carbon content of sandy soil |
| CN105706602A (en) * | 2016-01-26 | 2016-06-29 | 青岛农业大学 | Method for influencing moisture soil nitrogen mineralization characteristics and crop yield by long-term positioned fertilization |
| CN106596536A (en) * | 2016-12-15 | 2017-04-26 | 西南大学 | Natural particle size soil nitrogen grading method |
| CN107941987A (en) * | 2017-11-23 | 2018-04-20 | 上海国齐检测技术有限公司 | The assay method of full nitrogen in a kind of soil |
| CN108760563A (en) * | 2018-05-30 | 2018-11-06 | 吴惠丽 | A kind of chemical fertilizer device for detecting water content |
| CN109115939A (en) * | 2018-09-29 | 2019-01-01 | 大丰跃龙化学有限公司 | A kind of method of quick measurement cyclopropyl amine content |
| CN109254111A (en) * | 2018-10-19 | 2019-01-22 | 上海永通生态工程股份有限公司 | A kind of detection method and preparation method thereof of Water soluble fertilizer nitrate nitrogen content |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101726448A (en) * | 2008-10-10 | 2010-06-09 | 中国科学院沈阳应用生态研究所 | Method for monitoring organic matter remaining quantity in soil sample |
| US20100139378A1 (en) * | 2008-12-05 | 2010-06-10 | Schlumberger Technology Corporation | Method for determination of pore water content in equilibrium with gas hydrate in dispersed media |
| US20100283993A1 (en) * | 2009-05-07 | 2010-11-11 | Solum, Inc. | Automated Soil Measurement Device |
| CN102297799A (en) * | 2011-07-19 | 2011-12-28 | 中国科学院东北地理与农业生态研究所 | Method for leaching nitrate nitrogen in soil |
| CN102435560A (en) * | 2011-09-06 | 2012-05-02 | 深圳市喜耕田生物科技股份有限公司 | Method for testing nitrification inhibition rate of stabilized fertilizer |
-
2014
- 2014-10-28 CN CN201410585730.2A patent/CN104330326A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101726448A (en) * | 2008-10-10 | 2010-06-09 | 中国科学院沈阳应用生态研究所 | Method for monitoring organic matter remaining quantity in soil sample |
| US20100139378A1 (en) * | 2008-12-05 | 2010-06-10 | Schlumberger Technology Corporation | Method for determination of pore water content in equilibrium with gas hydrate in dispersed media |
| US20100283993A1 (en) * | 2009-05-07 | 2010-11-11 | Solum, Inc. | Automated Soil Measurement Device |
| CN102297799A (en) * | 2011-07-19 | 2011-12-28 | 中国科学院东北地理与农业生态研究所 | Method for leaching nitrate nitrogen in soil |
| CN102435560A (en) * | 2011-09-06 | 2012-05-02 | 深圳市喜耕田生物科技股份有限公司 | Method for testing nitrification inhibition rate of stabilized fertilizer |
Non-Patent Citations (3)
| Title |
|---|
| C.F.H.LIAO: "测定全氮的代氏合金法", 《土壤学进展》 * |
| 王群 等: "集安地区土壤中氮含量的测定", 《中国农业信息》 * |
| 鲍俊丹 等: "13种土壤硝化过程中亚硝态氮的累积与土壤性质的关系", 《农业环境科学学报》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424871A (en) * | 2015-12-10 | 2016-03-23 | 苏州国环环境检测有限公司 | Determination method for ammonia nitrogen in wastewater |
| CN105527237A (en) * | 2015-12-16 | 2016-04-27 | 河南大学 | Method for determining carbon content of sandy soil |
| CN105706602A (en) * | 2016-01-26 | 2016-06-29 | 青岛农业大学 | Method for influencing moisture soil nitrogen mineralization characteristics and crop yield by long-term positioned fertilization |
| CN106596536A (en) * | 2016-12-15 | 2017-04-26 | 西南大学 | Natural particle size soil nitrogen grading method |
| CN106596536B (en) * | 2016-12-15 | 2019-05-31 | 西南大学 | Soil Nitrogen stage division under a kind of natural particle size |
| CN107941987A (en) * | 2017-11-23 | 2018-04-20 | 上海国齐检测技术有限公司 | The assay method of full nitrogen in a kind of soil |
| CN108760563A (en) * | 2018-05-30 | 2018-11-06 | 吴惠丽 | A kind of chemical fertilizer device for detecting water content |
| CN109115939A (en) * | 2018-09-29 | 2019-01-01 | 大丰跃龙化学有限公司 | A kind of method of quick measurement cyclopropyl amine content |
| CN109254111A (en) * | 2018-10-19 | 2019-01-22 | 上海永通生态工程股份有限公司 | A kind of detection method and preparation method thereof of Water soluble fertilizer nitrate nitrogen content |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104330326A (en) | Measuring method of nitrogen content in soil | |
| Han et al. | Effect of biochar on the soil nutrients about different grasslands in the Loess Plateau | |
| Taghizadeh-Toosi et al. | Biochar adsorbed ammonia is bioavailable | |
| Cai et al. | Nitrification and acidification from urea application in red soil (Ferralic Cambisol) after different long-term fertilization treatments | |
| Fangueiro et al. | Impact of cattle slurry acidification on carbon and nitrogen dynamics during storage and after soil incorporation | |
| Fernández-Delgado Juárez et al. | Wood ash effects on chemical and microbiological properties of digestate-and manure-amended soils | |
| CN104034681A (en) | Method for detecting content of ammonia nitrogen in sewage | |
| ATE13815T1 (en) | METHOD AND DEVICE FOR ADJUSTING THE NH3 CONTENT IN THE WASHING LIQUID OF A GAS WASHER. | |
| CN104330406A (en) | Method for measuring organic matters in soil | |
| Corrêa et al. | Biosolids effectiveness to yield ryegrass based on their nitrogen content | |
| CN102992831B (en) | Laying hen excrement composting method capable of reducing compost raw material ammonia production amount | |
| CN103723866A (en) | Method for recycling nitrogen and phosphorus from anaerobic fermentation liquid | |
| Premi et al. | Incubation study of nitrification of digested sewage sludge added to soil | |
| Meena et al. | Effect of FYM and sewage sludge application on yield and quality of pearl millet-mustard cropping system and soil fertility in a Typic Haplustept | |
| Middleton | The use of the orange I method for determining soil nitrates and a comparison with the phenol‐sulphonic acid method for certain soils of Northern Nigeria | |
| Kizewski et al. | Nitrate transformation and immobilization in particulate organic matter incubations: Influence of redox, iron and (a) biotic conditions | |
| Choi et al. | Nitrogen transformations and ammonia volatilization losses from 15N-urea as affected by the co-application of composted pig manure | |
| CN105503283B (en) | Method for producing high-nitrate-concentration organic fertilizer by utilizing composting reactor | |
| Cavalli et al. | Nitrogen availability after repeated additions of raw and anaerobically digested 15N‐labelled pig slurry | |
| Simonič et al. | Vacuum evaporation of liquid fraction of digestate | |
| Matheyarasu et al. | Assessment of nitrogen losses through nitrous oxide from abattoir wastewater-irrigated soils | |
| CN102030587B (en) | Method and fixative for controlling nitrogen loss in composting process | |
| Blessy et al. | Application of water hyacinth vermicompost on the growth of Capsicum annum | |
| CN107349913A (en) | A kind of heavy metals removal method based on PAL M materials in feces of livestock and poultry | |
| Możdżer et al. | Impact of natural fertilization using PRP fix on some soil fertility indicators |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150204 |