CN105527237A - Method for determining carbon content of sandy soil - Google Patents
Method for determining carbon content of sandy soil Download PDFInfo
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- CN105527237A CN105527237A CN201510947223.3A CN201510947223A CN105527237A CN 105527237 A CN105527237 A CN 105527237A CN 201510947223 A CN201510947223 A CN 201510947223A CN 105527237 A CN105527237 A CN 105527237A
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- carbon content
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- 239000002689 soil Substances 0.000 title claims abstract description 72
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000000243 solution Substances 0.000 claims abstract description 27
- 238000010790 dilution Methods 0.000 claims abstract description 10
- 239000012895 dilution Substances 0.000 claims abstract description 10
- 239000006228 supernatant Substances 0.000 claims abstract description 6
- 239000012286 potassium permanganate Substances 0.000 claims abstract description 5
- 238000005303 weighing Methods 0.000 claims abstract description 4
- 238000003556 assay Methods 0.000 claims description 30
- 238000002474 experimental method Methods 0.000 claims description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000004570 mortar (masonry) Substances 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 230000009514 concussion Effects 0.000 claims description 4
- 238000009825 accumulation Methods 0.000 claims description 3
- 230000031700 light absorption Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 238000011161 development Methods 0.000 abstract description 7
- 238000007605 air drying Methods 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 238000002835 absorbance Methods 0.000 abstract 3
- 238000007865 diluting Methods 0.000 abstract 1
- 230000035939 shock Effects 0.000 abstract 1
- 239000002344 surface layer Substances 0.000 abstract 1
- 239000002245 particle Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000004016 soil organic matter Substances 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 241001170716 Garra gotyla Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a method for determining the carbon content of sandy soil. The method includes the following steps: (1) collecting a fresh sample on a soil surface layer and a soil cross section, air-drying, then removing debris, and allowing the sample to pass through a soil sieve; (2) according to soil properties, weighing a proper amount of sample correspondingly; (3) mixing the weighed soil sample with a KMnO4 solution, and carrying out shock centrifugal treatment; (4) taking a proper amount of the supernatant after centrifugal treatment, and diluting and making the volume constant; (5) measuring the absorbance of the solution after dilution and constant volume realizing by an ultraviolet spectrophotometer; (6) preparing a plurality of KMnO4 solutions with different concentrations, measuring the absorbance with the same ultraviolet spectrophotometer respectively, and drawing a standard curve between the concentration and the absorbance; and (7) according to the standard curve, calculating to obtain the consumption amount of KMnO4, and then calculating to obtain the percentage of organic carbon. The determination of the organic carbon content in the soil is relatively accurate, a reference and a basis are provided for study on global climate change and regional agricultural development, and a role in promoting the development of agriculture and environment is played.
Description
Technical field
The invention belongs to environmental monitoring technology field, relate to a kind of assay method of sandy soil carbon content.
Background technology
In soil, organic carbon content has vital role to the maintenance of soil quality and the lifting of fertility, and the development that its dynamic change can be research Global climate change and regional agriculture provides reference and foundation.Soil organic matter is differed by multiple different in kind, molecular structure, decompose and compound that conversion rate is different forms, therefore, the content understanding and grasping soil organic matter and each constituent thereof has impetus to the development of agricultural and the environmental problem that causes.
In soil organic matter, the mensuration of active organic carbon and nonactive organic carbon content is climate change and the developing research contents of related discipline always.But because of soil types, the soil texture, organic carbon content difference, in mensuration process, the assay method of active organic carbon in soil content cannot be unified.Because active organic carbon content is low, there is the problems such as measurement result is inaccurate or do not measure.The soil that in, the soil texture lower for organic carbon content, sand grain content is high, the more difficult control of process of mensuration, experimental result is more inaccurate; Because sample introduction particle size is different and sample size all can cause the error between soil total organic carbon, active organic carbon and nonactive organic carbon content to increase.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of assay method reducing active organic carbon in soil experimental error, more accurately sandy soil carbon content, for relevant research provides reference data.
For achieving the above object, the invention provides following technical scheme: the method comprises the steps:
(1) gather the fresh sample on upper soll layer and section, take back laboratory air-dry after, remove foreign material, cross soil sieve;
(2) appropriate amount of sample is taken according to soil property correspondence;
(3) by the pedotheque of weighing and KMnO
4solution mixes and carries out concussion centrifugal treating;
(4) supernatant after centrifugal treating is got in right amount and dilute constant volume;
(5) under ultraviolet spectrophotometer, measure the absorptance of solution after dilution constant volume;
(6) KMnO of multiple variable concentrations is configured
4solution, measures its absorptance respectively under identical ultraviolet spectrophotometer, draws the typical curve between concentration and absorptance;
(7) according to the difference between blank assay and the light absorption value of pedotheque, KMnO is calculated from typical curve
4reacted concentration change, calculating K MnO
4consumption, according to KMnO
4with the reaction equation of organic carbon, by KMnO
4consumption calculate the percentage composition of organic carbon.
The assay method of described a kind of sandy soil carbon content, the method for step (1), for be ground by sandy soil agate mortar, then crosses soil sieve.
The assay method of described a kind of sandy soil carbon content, pedotheque in step (2) is the sandy soil of NORTHWEST CHINA arid biogeographic zone, sample size is at 5 ~ 10g, aeolian sandy soil, chisley soil, grey-brown desert soil pedotheque amount are at 10 ~ 15g, a large amount of silts of carrying of the Yellow River are at the pedotheque of the Yellow River middle and lower reaches Plain alluviation or accumulation, and in experiment, sampling amount is at 5 ~ 10g.
The assay method of described a kind of sandy soil carbon content, the KMnO4 solution added in step (3) is the KMnO of the 333mmol/L of 12.5ml
4solution.
The assay method of described a kind of sandy soil carbon content, the method for step (3) is under normal temperature, is placed by centrifuge tube on oscillator and shakes 1 hour, then at rotating speed be 4000rpm tacho in static after centrifugal 5 minutes.
The assay method of described a kind of sandy soil carbon content, step (4) for get 1ml concussion centrifugal treating after supernatant move in 250ml volumetric flask, dilution is fixed molten.
The assay method of described a kind of sandy soil carbon content, the absorptance in step (5) is measured under UV-3600 ultraviolet spectrophotometer 565nm.
The assay method of described a kind of sandy soil carbon content, the method for step (6) is the KMnO configuring 0.10mmol/l, 0.15mmol/l, 0.33mmol/l, 0.67mmol/l, 1.0mmol/l respectively
4solution, measures absorptance under UV-3600 ultraviolet spectrophotometer 565nm, draws the typical curve between concentration and absorptance;
The assay method of described a kind of sandy soil carbon content, the computing formula of the active organic carbon content of step (7) is
The assay method of described a kind of sandy soil carbon content, the computing formula of the active organic carbon content in step (6) can replace to
wherein, reacted concentration=250 × KMnO
4solution concentration value, KMnO
4solution concentration value is concentration value corresponding to the absorptance that records after dilution 250 times.
The assay method of described a kind of sandy soil carbon content, each pedotheque experiment repeats three times, averages.
Beneficial effect of the present invention is: more accurate to the mensuration of organic carbon content in soil, the organic carbon content measured in soil has vital role to the maintenance of soil quality and the lifting of fertility accurately, the development that its dynamic change can be research Global climate change and regional agriculture provides reference and foundation, plays a role in promoting to the development of agricultural and the development of environment.
Embodiment
In the assay method of sandy soil carbon content of the present invention, first the fresh sample of the 2kg on upper soll layer and section is gathered, after taking back laboratory natural air drying, the foreign material such as Litter removal, chad, with agate mortar, pedotheque is ground, avoid using glass and porcelain mortar.After the soil sieve of ground 2mm particle diameter, then the soil sieve of ground for sample 0.149mm particle diameter.What use in experiment is the pedotheque of 0.149mm.For reducing how many errors of causing because of sample size, on the different sections under same land use pattern or soil types, pedotheque sampling amount is consistent; Sampling amount under different land use type or soil types on section can according to how many increases and decreases of sand grain content.
Weigh the pedotheque after process.The sandy soil sample size of NORTHWEST CHINA arid biogeographic zone is between 5 ~ 10g, and the pedotheque amounts such as aeolian sandy soil, chisley soil, grey-brown desert soil are between 10 ~ 15g; A large amount of silts of carrying of the Yellow River are at the pedotheque of the Yellow River middle and lower reaches Plain alluviation or accumulation, and in experiment, sampling amount is between 5 ~ 10g.
Pedotheque after weighing is put into 100ml centrifuge tube.The KMnO that concentration is 333mmol/L is got with the Eppendorf pipettor of 10ml
4solution 12.5ml puts into soil sample.12.5mlKMnO
4when solution puts into soil, because pedotheque particle carefully easily flies upward, grogs is adsorbed by pipettor sucker, therefore, for preventing pedotheque from flying upward, is adding KMnO
4in process, pipettor sucker head is placed in 2-3cm place above pedotheque and slowly adds, and limit edged rotates centrifuge tube, treats KMnO
4after not having pedotheque, can improve and add speed.The wherein KMnO of 333mmol/L
4the collocation method of solution: because of KMnO
4bulk, not easily dissolves completely, for improving rate of dissolution, adopts following methods: (1) is before use by block KMnO
4grind in agate mortar; (2) KMnO of 52.625g is claimed
4powder is placed in 1000ml beaker, and after putting into the distilled water of 600ml-800ml, beaker is placed on magnetic stirring apparatus and stirs, and is surely dissolved in 1000ml volumetric flask until completely dissolved.
Under normal temperature, centrifuge tube is placed on oscillator and shakes 1 hour, then at rotating speed be 4000rpm tacho in centrifugal 5 minutes, the supernatant that the Eppendorf pipettor of static rear 5ml gets 1ml moves in 250ml volumetric flask, and dilution is calmly molten.
By the solution after dilution constant volume, under UV-3600 ultraviolet spectrophotometer 565nm, measure absorptance, record result.Meanwhile, the absorptance of soil blank assay is measured.
Configure 333mmol/lKMnO
4after solution, then configure the KMnO of 0.10mmol/l, 0.15mmol/l, 0.33mmol/l, 0.67mmol/l, 1.0mmol/l respectively
4solution, measures absorptance under UV-3600 ultraviolet spectrophotometer 565nm, draws the typical curve between concentration and absorptance.
According to the difference between blank assay and the light absorption value of pedotheque, calculate KMnO from typical curve
4reacted concentration change, calculating K MnO
4consumption.According to KMnO
4with the reaction equation of organic carbon, by KMnO
4consumption calculate the percentage composition of organic carbon.Chemical equation:
4KMnO
4+3C+H
2O＝4MnO
2+K
2CO
3+2KHCO
3
From above formula, in oxidizing process, the KMnO of 1mmol
40.75mmol (9mg) carbon can be consumed.
The computing formula of active organic carbon content is:
Or directly calculate with following formula
In formula, reacted concentration=250 × KMnO
4solution concentration value, KMnO
4solution concentration value is concentration value corresponding to the absorptance that records after dilution 250 times.
Each pedotheque experiment will repeat three times, averages.
What finally illustrate is, above preferred embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although by above preferred embodiment to invention has been detailed description, but those skilled in the art are to be understood that, various change can be made to it in the form and details, and not depart from claims of the present invention limited range.
Claims (11)
1. an assay method for sandy soil carbon content, is characterized in that: the method comprises the steps:
(1) gather the fresh sample on upper soll layer and section, take back laboratory air-dry after, remove foreign material, cross soil sieve;
(2) appropriate amount of sample is taken according to soil property correspondence;
(3) pedotheque of weighing mixed with KMnO4 solution and carry out concussion centrifugal treating;
(4) supernatant after centrifugal treating is got in right amount and dilute constant volume;
(5) under ultraviolet spectrophotometer, measure the absorptance of solution after dilution constant volume;
(6) KMnO of multiple variable concentrations is configured
4solution, measures its absorptance respectively under ultraviolet spectrophotometer phase co-wavelength, draws the typical curve between concentration and absorptance;
(7) according to the difference between blank assay and the light absorption value of pedotheque, KMnO is calculated from typical curve
4reacted concentration change, calculating K MnO
4consumption, according to KMnO
4with the reaction equation of organic carbon, by KMnO
4consumption calculate the percentage composition of organic carbon.
2. the assay method of a kind of sandy soil carbon content according to claim 1, is characterized in that: the method for step (1), for be ground by sandy soil agate mortar, then crosses soil sieve.
3. the assay method of a kind of sandy soil carbon content according to claim 2, it is characterized in that: the pedotheque in step (2) is the sandy soil of NORTHWEST CHINA arid biogeographic zone, sample size is at 5 ~ 10g, aeolian sandy soil, chisley soil, grey-brown desert soil pedotheque amount are at 10 ~ 15g, a large amount of silts of carrying of the Yellow River are at the pedotheque of the Yellow River middle and lower reaches Plain alluviation or accumulation, and in experiment, sampling amount is at 5 ~ 10g.
4. the assay method of a kind of sandy soil carbon content according to claim 3, is characterized in that: the KMnO added in step (3)
4solution is the KMnO of the 333mmol/L of 12.5ml
4solution.
5. the assay method of a kind of sandy soil carbon content according to claim 4, it is characterized in that: the method for step (3) is under normal temperature, centrifuge tube is placed on oscillator and shakes 1 hour, then at rotating speed be 4000rpm tacho in static after centrifugal 5 minutes.
6. the assay method of a kind of sandy soil carbon content according to claim 5, is characterized in that: step (4) for get 1ml concussion centrifugal treating after supernatant move in 250ml volumetric flask, dilution is fixed molten.
7. the assay method of a kind of sandy soil carbon content according to claim 6, is characterized in that: the absorptance in step (5) is measured under UV-3600 ultraviolet spectrophotometer 565nm.
8. the assay method of a kind of sandy soil carbon content according to claim 7, is characterized in that: the method for step (6) is the KMnO configuring 0.10mmol/l, 0.15mmol/l, 0.33mmol/l, 0.67mmol/l, 1.0mmol/l respectively
4solution, measures absorptance under UV-3600 ultraviolet spectrophotometer 565nm, draws the typical curve between concentration and absorptance.
9. the assay method of a kind of sandy soil carbon content according to claim 8, is characterized in that: the computing formula of the active organic carbon content of step (7) is
10. the assay method of a kind of sandy soil carbon content according to claim 9, is characterized in that: the computing formula of the active organic carbon content in step (6) can replace to
wherein, reacted concentration=250 × KMnO
4solution concentration value, KMnO
4solution concentration value is concentration value corresponding to the absorptance that records after dilution 250 times.
The assay method of 11. a kind of sandy soil carbon contents according to any one of claim 1 ~ 10, is characterized in that: each pedotheque experiment repeats three times, averages.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510947223.3A CN105527237A (en) | 2015-12-16 | 2015-12-16 | Method for determining carbon content of sandy soil |
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| CN201510947223.3A CN105527237A (en) | 2015-12-16 | 2015-12-16 | Method for determining carbon content of sandy soil |
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| Publication Number | Publication Date |
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| CN105527237A true CN105527237A (en) | 2016-04-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111076994A (en) * | 2019-12-16 | 2020-04-28 | 苏州易云生物科技有限公司 | Soil adjustment method based on biological detection |
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| CN111076994A (en) * | 2019-12-16 | 2020-04-28 | 苏州易云生物科技有限公司 | Soil adjustment method based on biological detection |
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