CN103940896A - Method for monitoring nitrogen dynamic state in soil - Google Patents
Method for monitoring nitrogen dynamic state in soil Download PDFInfo
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Abstract
The invention discloses a method for monitoring nitrogen dynamic state in soil. The method comprises the following steps: S10. preparing before sampling the soil; S20. sampling the soil and collecting a soil sample; S30. processing the collected soil sample to obtain the processed soil sample; S40. measuring the content of total nitrogen in the processed soil sample; S50. collecting the soil sample with the content of total nitrogen measured by the step S40; S60. measuring 15N abundance of the soil sample collected by the step S50 by using an isotope mass spectrometer; S70. obtaining the nitrogen dynamic state in the soil according to the 15N abundance. The method for monitoring nitrogen dynamic in soil can be used for sampling the soil, measuring the content of the total nitrogen of the soil sample and the 15N abundance, and monitoring the nitrogen dynamic state in the soil by using the characteristic of 15N/14N assimilation differences in the biological nitrogen fixation of nitrogen-fixing plants and fully using the characteristic of natural mark on the nitrogen dynamic through the stable isotope 15N.
Description
Technical field
The present invention relates to a kind of monitoring method of material recycle of soil, relate in particular to a kind of dynamic method of monitoring Soil Nitrogen.
Background technology
Nitrogen cycle is one of the most basic material recycle of the ecosystem, and the 26S Proteasome Structure and Function of the ecosystem is played to important regulating action.As one of required macronutrient of plant growth and growth, nitrogen mainly comes from soil organic debris in soil, after biological process mineralising, absorbs, thereby complete its growth and development process for plant; And nitrogen element is also the mineral element of plant uptake maximum from soil.Visible, the dynamic profound influence of Soil Nitrogen the succession of community and the bio-diversity of vegetation.In recent years, the exhaustive exploitation utilization of fossil fuel and the change of Land-Use, caused the change of Global climate change and carbon nitrogen cycle.Along with the aggravation of mankind's activity on ecosystem impact, nitrogen cycle, carbon cycle and climate change interphase interaction and relation show its important decisive role day by day to the development of the ecosystem and succession.
Soil Nitrogen is dynamically the important component part of ecosystem nitrogen cycle.On the one hand, the nitrogen in atmosphere enters in soil through biological nitrogen fixation and nitrogen deposition effect.Plant absorbs nitrogen from soil by root system, meet the demand that it grows.Soil microorganism can be reduced into nitrogen by the nitrogen in soil in a short time and be returned in atmosphere, but on long terms, but can promote that the nitrogen in soil is fixed in soil with organic form.On the other hand, the nitrogen in atmosphere also can be converted into other compound by industry, imposes in soil, participates in Soil Nitrogen cyclic process equally via effects such as plant and microorganisms.Soil Nitrogen is dynamically also in all many-sided cyclic process, especially carbon cycles that affects and regulating and controlling other material or nutrient, thus profound influence global carbon, and indirectly-acting is in changing because of carbon cycle the Global climate change causing.So far, conversion and the whereabouts of nitrogen in soil become one of focus of scientific research.
Summary of the invention
The object of the invention is to provide a kind of dynamic method of monitoring Soil Nitrogen.
Technical solution problem of the present invention adopts following technical scheme: a kind of dynamic method of monitoring Soil Nitrogen, comprises the following steps:
Before S10, soil sampling, prepare;
S20, soil is carried out to sampling operation, gather soil sample;
S30, the soil sample gathering is processed to the soil sample after being processed;
The total nitrogen content of the soil sample after S40, measurement are processed;
S50, collection step S40 measure the soil sample after total nitrogen content;
S60, utilize isotope mass spectrometer to measure soil sample that described step S50 collects
15n abundance;
Described in S70, basis
15it is dynamic that N abundance obtains Soil Nitrogen.
Optionally, described step S10 is specially:
S101, selection sampling soil: select soil background close, and the soil of the He Fei of the nitrogen-fixing plants group nitrogen-fixing plants group of growing is respectively as sampling soil;
S103, sampled point are determined: according to accidental sampling, in sampling soil, choose sampled point;
S105, sampling thief are selected: sampling thief is chosen as the earth boring auger of diameter 2cm; And select earth boring auger drilling rod and drill bit according to sampling depth.
Optionally, step S20 is specially:
S201, earth boring auger is vertically pressed in soil, until drill bit arrives or surpasses sampling depth;
S203,360 ° of rotation earth boring augers, the earth pillar in cut-out earth boring auger is connected with the soil body;
S205, vertically extract earth boring auger;
S207, use are repaiied native cutter by the earth pillar layering in earth boring auger, and the soil sample of different layers is respectively charged in valve bag.
Optionally, described step S30 is specially:
S301, that the soil sample collecting is placed in to shady and cool ventilation place is air-dry, and the soil sample after air-dry is ground;
S303, the soil sample after grinding is crossed to 1mm sieve, the soil sample after sieving is mixed;
S305, take 5 grams of soil samples after mixing, and measure alleged water cut of getting soil sample;
S307, by taking the soil sample mixing after 5 grams of soil samples, cross 0.149mm sieve, the soil sample of crossing after 0.149mm sieve is mixed; Soil sample after being processed.
Optionally, described step S40 is specially:
S401, take 1 gram of soil sample after processing, and 1 gram of taken soil sample is disappeared and boiled;
S403, the soil sample offseting after boiling distill, and collect the distillate of ammonia;
S405, by the titration to the distillate of ammonia, the total nitrogen content of the soil sample after being processed.
Optionally, described step S70 is specially: the total nitrogen content of the soil sample of the contrast He Fei of nitrogen-fixing plants group nitrogen-fixing plants group and
15n abundance, obtains Soil Nitrogen dynamic.
Optionally, the method for measuring the total nitrogen content of the soil sample after processing in described step S40 is open circulit ethods.
The present invention has following beneficial effect: the dynamic method of monitoring Soil Nitrogen of the present invention comprise to soil sample, measure soil sample total nitrogen content and
15n abundance, utilizes the biological nitrogen fixation pair of nitrogen-fixing plants
15n/
14the characteristic of N assimilation difference, makes full use of stable isotope
15n is to the dynamic Natural check feature of nitrogen, and monitoring Soil Nitrogen is dynamic.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is further elaborated.
Embodiment 1
The present embodiment provides a kind of monitoring Soil Nitrogen dynamic method, comprises the following steps:
Before S10, soil sampling, prepare;
S20, soil is carried out to sampling operation, gather soil sample;
S30, the soil sample gathering is processed to the soil sample after being processed;
The total nitrogen content of the soil sample after S40, measurement are processed;
S50, collection step S40 measure the soil sample after total nitrogen content;
S60, utilize isotope mass spectrometer to measure soil sample that described step S50 collects
15n abundance;
Described in S70, basis
15it is dynamic that N abundance obtains Soil Nitrogen.
The dynamic method of monitoring Soil Nitrogen of the present invention comprise to soil sample, measure soil sample total nitrogen content and
15n abundance, utilizes the biological nitrogen fixation pair of nitrogen-fixing plants
15n/
14the characteristic of N assimilation difference, makes full use of stable isotope
15n is to the dynamic Natural check feature of nitrogen, and monitoring Soil Nitrogen is dynamic.
In the present embodiment, optional, described step S10 is specially:
S101, selection sampling soil: select soil background close, and the soil of the He Fei of the nitrogen-fixing plants group nitrogen-fixing plants group of growing is respectively as sampling soil; Be nitrogen-fixing plants and non-nitrogen-fixing plants alternating growth, and complete the soil of three circulations;
S103, sampled point are determined: according to accidental sampling, in sampling soil, choose sampled point;
S105, sampling thief are selected: sampling thief is chosen as the earth boring auger of diameter 2cm; And select earth boring auger drilling rod and drill bit according to sampling depth, and with the selection by soil sample, realize the dynamic precise monitoring of Soil Nitrogen, improve the confidence level of result.
In the present embodiment, optional, step S20 is specially:
S201, earth boring auger is vertically pressed in soil, until drill bit arrives or surpasses sampling depth;
S203,360 ° of rotation earth boring augers, the earth pillar in cut-out earth boring auger is connected with the soil body;
S205, vertically extract earth boring auger;
S207, with repairing native cutter by the earth pillar layering in earth boring auger, the soil sample of different layers is respectively charged in valve bag, by the collection to soil sample, obtain the soil sample of different layers, thereby provide the foundation for the measurement of the nitrogen element content in follow-up soil.
In the present embodiment, optional, described step S30 is specially:
S301, that the soil sample collecting is placed in to shady and cool ventilation place is air-dry, and the soil sample after air-dry is ground;
S303, the soil sample after grinding is crossed to 1mm sieve, the soil sample after sieving is mixed;
S305, take 5 grams of soil samples after mixing, and measure alleged water cut of getting soil sample;
S307, by taking the soil sample mixing after 5 grams of soil samples, cross 0.149mm sieve, the soil sample of crossing after 0.149mm sieve is mixed; Soil sample after being processed, with the accuracy of the data by the follow-up full nitrogen measurement of these treatment steps.
In the present embodiment, optional, described step S40 is specially:
S401, take 1 gram of soil sample after processing, and 1 gram of taken soil sample is disappeared and boiled;
S403, the soil sample offseting after boiling distill, and collect the distillate of ammonia;
S405, by the titration to the distillate of ammonia, the total nitrogen content of the soil sample after being processed, and preserve the distillate that is titrated to terminal, to measure the total nitrogen content of soil sample, and for monitoring
15the abundance of N is ready.
In the present embodiment, optional, the distillate that is titrated to terminal is concentrated into volume and is no more than 3ml, utilize isotope mass spectrometer to measure in concentrate
15the content of N, thus realize right
15the detection of N abundance.
In the present embodiment, optional, described step S70 is specially: the total nitrogen content of the soil sample of the contrast He Fei of nitrogen-fixing plants group nitrogen-fixing plants group and
15n abundance, obtains Soil Nitrogen dynamic.
In the present embodiment, optional, the method for measuring the total nitrogen content of the soil sample after processing in described step S40 is open circulit ethods, to facilitate the detection realizing the total nitrogen content of soil sample.
Nitrogen-fixing plants in the dynamic method of monitoring Soil Nitrogen of the present invention can be converted into the nitrogen in atmosphere after ammonia or nitrate in a large number by biological nitrogen fixation, are fixed in soil.These are fixed to the nitrogen in soil, on the one hand, participate in Soil Nitrogen circulation directly, as organic formation etc.; On the other hand, for plant, absorb, meet the demand to nitrogen in its growth and development process, but finally this part nitrogen is given back in soil with the form of plant residue or dead body again, participate in Soil Nitrogen circulation.Due to atmospheric nitrogen
15n component is significantly lower than Soil Nitrogen
15n component.Visible, on long terms, the biological nitrogen fixation pair of nitrogen-fixing plants
14n/
15the assimilation difference of N can cause the nitrogen in atmosphere to enter in large quantities in soil and the nitrogen cycle of participating, thereby makes Soil Nitrogen
15n component significantly reduces, and Soil Nitrogen is dynamically played to mark effect.Therefore, can utilize nitrogen-fixing plants pair
14n/
15this assimilation difference of N can reach the dynamic object of monitoring Soil Nitrogen.
The present invention has the following advantages and beneficial effect:
1, result is accurately reliable: due to stable isotope
15the content of N component in atmospheric nitrogen is lower than Soil Nitrogen, and nitrogen-fixing plants mainly assimilate by biological nitrogen fixation the nitrogen absorbing in atmosphere, thus form right
15n/
14the assimilation difference of N.Nitrogen through nitrogen-fixing plants assimilations enters in soil, reduced Soil Nitrogen
15n component concentration, participates in Soil Nitrogen circulation simultaneously, thereby plays the dynamic precise monitoring of Soil Nitrogen, and credible result degree is high.
2, operate distinct succinct, saving of work and time: utilize earth boring auger directly to take soil sample, air-dry in shady and cool ventilation place after layering, grind and sieve; The soil sample that takes certain mass disappears and boils, and disappears and boils rear distillation, collects distillate; Titration distillate to terminal, records vs consumption; The distillate that is titrated to terminal is concentrated into volume and is no more than 3ml, then utilize isotope mass spectrometer to measure in concentrate
15n content, calculate total nitrogen content of soil and
15n abundance, thus show that Soil Nitrogen is dynamic; The dynamic method operation of visible monitoring Soil Nitrogen of the present invention is comparatively simple, and index measurement is less, and saves a large amount of artificial and time.
The sequencing of above embodiment only, for ease of describing, does not represent the quality of embodiment.
Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to previous embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (7)
1. the dynamic method of monitoring Soil Nitrogen, is characterized in that, comprises the following steps:
Before S10, soil sampling, prepare;
S20, soil is carried out to sampling operation, gather soil sample;
S30, the soil sample gathering is processed to the soil sample after being processed;
The total nitrogen content of the soil sample after S40, measurement are processed;
S50, collection step S40 measure the soil sample after total nitrogen content;
S60, utilize isotope mass spectrometer to measure soil sample that described step S50 collects
15n abundance;
Described in S70, basis
15it is dynamic that N abundance obtains Soil Nitrogen.
2. the dynamic method of monitoring Soil Nitrogen according to claim 1, is characterized in that, described step S10 is specially:
S101, selection sampling soil: select soil background close, and the soil of the He Fei of the nitrogen-fixing plants group nitrogen-fixing plants group of growing is respectively as sampling soil;
S103, sampled point are determined: according to accidental sampling, in sampling soil, choose sampled point;
S105, sampling thief are selected: sampling thief is chosen as the earth boring auger of diameter 2cm; And select earth boring auger drilling rod and drill bit according to sampling depth.
3. the dynamic method of monitoring Soil Nitrogen according to claim 2, is characterized in that, step S20 is specially:
S201, earth boring auger is vertically pressed in soil, until drill bit arrives or surpasses sampling depth;
S203,360 ° of rotation earth boring augers, the earth pillar in cut-out earth boring auger is connected with the soil body;
S205, vertically extract earth boring auger;
S207, use are repaiied native cutter by the earth pillar layering in earth boring auger, and the soil sample of different layers is respectively charged in valve bag.
4. the dynamic method of monitoring Soil Nitrogen according to claim 3, is characterized in that, described step S30 is specially:
S301, that the soil sample collecting is placed in to shady and cool ventilation place is air-dry, and the soil sample after air-dry is ground;
S303, the soil sample after grinding is crossed to 1mm sieve, the soil sample after sieving is mixed;
S305, take 5 grams of soil samples after mixing, and measure alleged water cut of getting soil sample;
S307, by taking the soil sample mixing after 5 grams of soil samples, cross 0.149mm sieve, the soil sample of crossing after 0.149mm sieve is mixed; Soil sample after being processed.
5. the dynamic method of monitoring Soil Nitrogen according to claim 4, is characterized in that, described step S40 is specially:
S401, take 1 gram of soil sample after processing, and 1 gram of taken soil sample is disappeared and boiled;
S403, the soil sample offseting after boiling distill, and collect the distillate of ammonia;
S405, by the titration to the distillate of ammonia, the total nitrogen content of the soil sample after being processed.
6. the dynamic method of monitoring Soil Nitrogen according to claim 1, is characterized in that, described step S70 is specially: the total nitrogen content of the soil sample of the contrast He Fei of nitrogen-fixing plants group nitrogen-fixing plants group and
15n abundance, obtains Soil Nitrogen dynamic.
7. the dynamic method of monitoring Soil Nitrogen according to claim 1, is characterized in that, the method for measuring the total nitrogen content of the soil sample after processing in described step S40 is open circulit ethods.
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| CN105527237A (en) * | 2015-12-16 | 2016-04-27 | 河南大学 | Method for determining carbon content of sandy soil |
| CN106226331A (en) * | 2016-07-18 | 2016-12-14 | 辽宁石油化工大学 | A kind of method measuring bulk soil soil denitrification |
| CN106556688A (en) * | 2016-11-25 | 2017-04-05 | 辽宁石油化工大学 | A kind of assay method of rhizosphere area soil ammonification |
| CN106556667A (en) * | 2016-11-25 | 2017-04-05 | 辽宁石油化工大学 | A kind of assay method of rhizosphere area soil nitrogen fixation |
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| CN106769623A (en) * | 2016-11-25 | 2017-05-31 | 辽宁石油化工大学 | A kind of assay method of rhizosphere area soil cellulysis |
| CN106771054A (en) * | 2016-11-25 | 2017-05-31 | 辽宁石油化工大学 | A kind of method of measure bulk soil soil cellulysis |
| CN106771055A (en) * | 2016-11-25 | 2017-05-31 | 辽宁石油化工大学 | A kind of method of measure bulk soil soil nitrogen fixation |
| CN112858449A (en) * | 2021-01-12 | 2021-05-28 | 中国科学院南京土壤研究所 | Method for determining nitrogen fixation potential of rice field soil organisms |
| CN114397424A (en) * | 2021-12-20 | 2022-04-26 | 中国科学院南京土壤研究所 | Soil ammonia determination method for determining natural abundance of nitrogen isotopes |
| CN115615742A (en) * | 2022-12-14 | 2023-01-17 | 沧州市生态环境监控中心(沧州市机动车污染防治监控中心) | Detection device for soil environment |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105527237A (en) * | 2015-12-16 | 2016-04-27 | 河南大学 | Method for determining carbon content of sandy soil |
| CN106226331A (en) * | 2016-07-18 | 2016-12-14 | 辽宁石油化工大学 | A kind of method measuring bulk soil soil denitrification |
| CN106556688A (en) * | 2016-11-25 | 2017-04-05 | 辽宁石油化工大学 | A kind of assay method of rhizosphere area soil ammonification |
| CN106556667A (en) * | 2016-11-25 | 2017-04-05 | 辽宁石油化工大学 | A kind of assay method of rhizosphere area soil nitrogen fixation |
| CN106644834A (en) * | 2016-11-25 | 2017-05-10 | 辽宁石油化工大学 | Method for determining ammonification of soil in non-rhizosphere region |
| CN106769623A (en) * | 2016-11-25 | 2017-05-31 | 辽宁石油化工大学 | A kind of assay method of rhizosphere area soil cellulysis |
| CN106771054A (en) * | 2016-11-25 | 2017-05-31 | 辽宁石油化工大学 | A kind of method of measure bulk soil soil cellulysis |
| CN106771055A (en) * | 2016-11-25 | 2017-05-31 | 辽宁石油化工大学 | A kind of method of measure bulk soil soil nitrogen fixation |
| CN112858449A (en) * | 2021-01-12 | 2021-05-28 | 中国科学院南京土壤研究所 | Method for determining nitrogen fixation potential of rice field soil organisms |
| CN114397424A (en) * | 2021-12-20 | 2022-04-26 | 中国科学院南京土壤研究所 | Soil ammonia determination method for determining natural abundance of nitrogen isotopes |
| CN115615742A (en) * | 2022-12-14 | 2023-01-17 | 沧州市生态环境监控中心(沧州市机动车污染防治监控中心) | Detection device for soil environment |
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