CN103226097A - Determination method for active organic carbon in soil - Google Patents
Determination method for active organic carbon in soil Download PDFInfo
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- CN103226097A CN103226097A CN2013100548261A CN201310054826A CN103226097A CN 103226097 A CN103226097 A CN 103226097A CN 2013100548261 A CN2013100548261 A CN 2013100548261A CN 201310054826 A CN201310054826 A CN 201310054826A CN 103226097 A CN103226097 A CN 103226097A
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Abstract
The invention discloses a determination method for active organic carbon in soil. The determination method comprises the steps of weighing 1.5 g of an air-dried soil sample screened by a 0.25 mm sieve in 100 mL of a plastic bottle, adding 25 mL of 333 mmol/L potassium permanganate, oscillating for 1 hour, taking 1-2 mL of a solution in a centrifugal tube, centrifuging for 2 min by a small high-speed centrifuge, transferring 100 [mu]L of a supernatant by a pipette, adding 25 mL of deionized water, mixing uniformly, then performing a colorimetric assay of a diluents on a spectrophotometer at a wavelength of 565 nm, and determining the active organic carbon of the sample according to the concentration of potassium permanganate. The method provided by the invention has the following advantages that 1. water consumption is small, so that cost is saved; 2. the speed is fast, so that large sample data can be analyzed; 2. deviations of analysis results caused by decomposition of the potassium permanganate are reduced; and 4. environment in a laboratory is kept relatively easily.
Description
Technical field
The invention belongs to soil activation organic carbon determination technical field, relate in particular to a kind of soil activation Method for Measuring Organic Carbon method.
Background technology
At present, soil activation Method for Measuring Organic Carbon method is: active organic carbon is measured and is adopted potassium permanganate oxidation method.The air-dry soil sample 1.5g that took by weighing the 0.25mm sieve is in the 100ml centrifuge tube, add 333mmol/L potassium permanganate 25mL, vibrated 1 hour, centrifugal 5 minutes (rotating speed 2000 commentaries on classics/min), get supernatant and dilute by 1:250 with deionized water, then with dilution in the 565nm colorimetric.Obtain the active organic carbon of sample according to the variation of potassium permanganate concentration.
This soil activation Method for Measuring Organic Carbon method water consumption is big; Speed is slow, and unit sample is long required analysis time, causes every day sample analysis quantity few; Centrifugal process, and subsequent step in easily cause potassium permanganate oxidation to decompose because of the time is long, thereby have influence on analysis result; In analysis, easily cause laboratory environment filthy because of potassium permanganate.
Summary of the invention
The purpose of the embodiment of the invention is to provide a kind of soil activation Method for Measuring Organic Carbon method, and it is big to be intended to solve present soil activation Method for Measuring Organic Carbon method water consumption; Speed is slow, and unit sample is long required analysis time, causes every day sample analysis quantity few; Centrifugal process, and subsequent step in easily cause potassium permanganate oxidation to decompose because of the time is long, thereby have influence on analysis result; In analysis, easily cause the filthy problem of laboratory environment because of potassium permanganate.
The embodiment of the invention is achieved in that a kind of soil activation Method for Measuring Organic Carbon method, and this assay method comprises:
The air-dry soil sample 1.5g that took by weighing the 0.25mm sieve adds 333mmol/L potassium permanganate 25mL in the 100ml plastic bottle, vibrated blank vibrated 1 hour the same with pedotheque 1 hour;
Get 1-2ml solution in the 2ml centrifuge tube, adopt the centrifugal 2min of small-sized high speed centrifugal machine, adopt liquid-transfering gun to pipette supernatant 100 μ l, add deionized water 25ml mixing, then with dilution colorimetric on the 565nm spectrophotometer, obtain the consumption of potassium permanganate according to the variation of potassium permanganate concentration, further obtain the active organic carbon content in the pedotheque.
Further, concrete computing formula is:
The content of soil activation organic carbon (mg/g)=[25 * (333-V
1A/V
2) ÷ 1000] * 9/ soil heavy (g)
Wherein: V
1---for diluting back KMnO
4Volume
A---for diluting back KMnO
4Concentration
V
2---for drawing the volume of supernatant
9---consume 1mmol KMnO
4The reduction coefficient that is equivalent to 9mg carbon
333---be KMnO
4Concentration.
Further, adopt the small-sized high speed centrifugal machine, 4000-10000 all can; Centrifugal 2min adopts the liquid-transfering gun of 100 μ l to pipette supernatant 100 μ l.
Method provided by the invention has the following advantages:
1. water consumption is few, saves cost;
2. speed is fast, can the large sample analysis data;
3. less potassium permanganate oxidation decomposes and the analysis result deviation that causes;
4. easily keep breadboard environment.
Figure of description
Fig. 1 is the effect contrast figure of the soil activation Method for Measuring Organic Carbon method that provides of the embodiment of the invention.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer,, the present invention is further elaborated below in conjunction with drawings and Examples.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in qualification the present invention.
The embodiment of the invention provides a kind of soil activation Method for Measuring Organic Carbon method, and concrete steps are:
The air-dry soil sample 1.5g that took by weighing the 0.25mm sieve adds 333mmol/L potassium permanganate 25mL in the 100ml plastic bottle, vibrated blank vibrated 1 hour the same with pedotheque 1 hour.Get 1-2ml solution in the 2ml centrifuge tube, adopt the centrifugal 2min of small-sized high speed centrifugal machine, adopt liquid-transfering gun to pipette supernatant 100 μ l, add deionized water 25ml mixing, then with dilution colorimetric on the 565nm spectrophotometer, obtain the consumption of potassium permanganate according to the variation of potassium permanganate concentration, further obtain the active organic carbon content in the pedotheque.
Concrete computing formula is:
The content of soil activation organic carbon (mg/g)=[25 * (333-V
1A/V
2) ÷ 1000] * 9/ soil heavy (g)
Wherein: V
1---for diluting back KMnO
4Volume
A---for diluting back KMnO
4Concentration
V
2---for drawing the volume of supernatant
9---consume 1mmol KMnO
4The reduction coefficient that is equivalent to 9mg carbon
333---be KMnO
4Concentration.
By two kinds of methods 60 soil samples are measured, the result as shown in Figure 1, two kinds of methods have extremely strong correspondence, correlativity reaches r=0.994, the standard deviation of twice mensuration of each sample shows that at 0.01-0.58 improved method can substitute classic method fully.
The above only is preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of being done within the spirit and principles in the present invention, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. soil activation Method for Measuring Organic Carbon method is characterized in that this assay method comprises:
The air-dry soil sample 1.5g that took by weighing the 0.25mm sieve adds 333mmol/L potassium permanganate 25mL in the 100ml plastic bottle, vibrated blank vibrated 1 hour the same with pedotheque 1 hour;
Get 1-2ml solution in the 2ml centrifuge tube, adopt the centrifugal 2min of small-sized high speed centrifugal machine, adopt liquid-transfering gun to pipette supernatant 100 μ l, add deionized water 25ml mixing, then with dilution colorimetric on the 565nm spectrophotometer, obtain the consumption of potassium permanganate according to the variation of potassium permanganate concentration, further obtain the active organic carbon content in the pedotheque.
2. soil activation Method for Measuring Organic Carbon method as claimed in claim 1 is characterized in that, concrete computing formula is:
The content of soil activation organic carbon (mg/g)=[25 * (333-V
1A/V
2) ÷ 1000] * 9/ soil heavy (g)
Wherein: V
1---for diluting back KMnO
4Volume
A---for diluting back KMnO
4Concentration
V
2---for drawing the volume of supernatant
9---consume 1mmol KMnO
4The reduction coefficient that is equivalent to 9mg carbon
333---be KMnO
4Concentration.
3. soil activation Method for Measuring Organic Carbon method as claimed in claim 1 is characterized in that, adopts the small-sized high speed centrifugal machine, and 4000-10000 all can; Centrifugal 2min adopts the liquid-transfering gun of 100 μ l to pipette supernatant 100 μ l.
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| CN2013100548261A CN103226097A (en) | 2013-02-20 | 2013-02-20 | Determination method for active organic carbon in soil |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712923A (en) * | 2013-12-23 | 2014-04-09 | 浙江大学 | Method for eliminating moisture influence factor in field in-situ soil measurement spectrums |
| CN105527237A (en) * | 2015-12-16 | 2016-04-27 | 河南大学 | Method for determining carbon content of sandy soil |
| CN112284867A (en) * | 2020-09-23 | 2021-01-29 | 西北农林科技大学 | Method for separating and extracting soil residual micro-plastic |
Citations (5)
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| RU2060948C1 (en) * | 1992-05-07 | 1996-05-27 | Агрофизический научно-исследовательский институт | Method of estimation of organic carbon and nitrogen change balance in the soil and the ratio of these elements |
| CN1570021A (en) * | 2004-04-28 | 2005-01-26 | 浙江大学 | Soil organic pollution rehabilitation method by using surfactant to increasing adsorption and fixing |
| CN101419175A (en) * | 2007-10-24 | 2009-04-29 | 中国科学院沈阳应用生态研究所 | Method for detecting organic matter in soil |
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2013
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Patent Citations (5)
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| CN1570021A (en) * | 2004-04-28 | 2005-01-26 | 浙江大学 | Soil organic pollution rehabilitation method by using surfactant to increasing adsorption and fixing |
| CN101419175A (en) * | 2007-10-24 | 2009-04-29 | 中国科学院沈阳应用生态研究所 | Method for detecting organic matter in soil |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712923A (en) * | 2013-12-23 | 2014-04-09 | 浙江大学 | Method for eliminating moisture influence factor in field in-situ soil measurement spectrums |
| CN105527237A (en) * | 2015-12-16 | 2016-04-27 | 河南大学 | Method for determining carbon content of sandy soil |
| CN112284867A (en) * | 2020-09-23 | 2021-01-29 | 西北农林科技大学 | Method for separating and extracting soil residual micro-plastic |
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Application publication date: 20130731 |