CN104328154A - Method for producing (S)-phenyl methanol from blue mold - Google Patents
Method for producing (S)-phenyl methanol from blue mold Download PDFInfo
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- CN104328154A CN104328154A CN201410594403.3A CN201410594403A CN104328154A CN 104328154 A CN104328154 A CN 104328154A CN 201410594403 A CN201410594403 A CN 201410594403A CN 104328154 A CN104328154 A CN 104328154A
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- 229960004217 benzyl alcohol Drugs 0.000 title abstract description 3
- 235000002233 Penicillium roqueforti Nutrition 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 239000000758 substrate Substances 0.000 claims abstract description 57
- 238000006243 chemical reaction Methods 0.000 claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 18
- 241000228143 Penicillium Species 0.000 claims abstract description 15
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 42
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 36
- GCSHUYKULREZSJ-UHFFFAOYSA-N phenyl(pyridin-2-yl)methanone Chemical compound C=1C=CC=NC=1C(=O)C1=CC=CC=C1 GCSHUYKULREZSJ-UHFFFAOYSA-N 0.000 claims description 19
- 239000000243 solution Substances 0.000 claims description 16
- 230000002210 biocatalytic effect Effects 0.000 claims description 12
- 238000009423 ventilation Methods 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 208000012839 conversion disease Diseases 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 229960003487 xylose Drugs 0.000 claims description 7
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 6
- 238000003810 ethyl acetate extraction Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
- 239000002953 phosphate buffered saline Substances 0.000 claims description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 6
- 229920000053 polysorbate 80 Polymers 0.000 claims description 6
- 230000035484 reaction time Effects 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000006555 catalytic reaction Methods 0.000 abstract description 9
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000003054 catalyst Substances 0.000 abstract description 2
- UYESUYBXKHPUDU-LBPRGKRZSA-N (s)-phenyl(pyridin-2-yl)methanol Chemical compound C1([C@H](O)C=2N=CC=CC=2)=CC=CC=C1 UYESUYBXKHPUDU-LBPRGKRZSA-N 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 abstract 1
- 241000228150 Penicillium chrysogenum Species 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000004927 clay Substances 0.000 description 3
- 239000005515 coenzyme Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000012847 fine chemical Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000024287 Areas Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 240000008564 Boehmeria nivea Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000790917 Dioxys <bee> Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 101710157860 Oxydoreductase Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 238000011914 asymmetric synthesis Methods 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Abstract
The invention relates to a method for producing (S)-phenyl methanol from blue mold, which comprises the following steps: preparing photoactive (S)-phenyl-(2-pyridyl)methanol by using penicillium cell as a biological catalyst, adding a phosphate buffer solution into the reaction tank, and adding argil to regulate the concentrations of the substrate and product. The penicillium cell is utilized to perform biological catalytic reaction, so the method has the advantages of high product yield and high enantiomeric excess ratio (ee%) and has favorable application prospects.
Description
Technical field
The invention belongs to technology of pharmaceutical engineering field, be related specifically to the technology that Penicillium cell biocatalysis prepares chiral medicinal intermediate (S)-phenyl-(2-pyridyl) methyl alcohol.
Background technology
Chirality (S)-phenyl-(2-pyridyl) methyl alcohol is the important intermediate of synthesis of chiral medicine, fine chemicals, agricultural chemicals product and other exotic materialss.Biocatalysis asymmetric reaction has environmental friendliness, mild condition, selectivity advantages of higher, prepares the first approach of hydroxyl chipal compounds as green high-efficient, is applied to the chipal compounds producing some high added values more and more.Biocatalysis is prepared chirality (S)-phenyl-(2-pyridyl) methyl alcohol and is had good application prospect.
Biocatalysis has the outstanding advantages such as catalytic efficiency is high, selectivity strong, mild condition, environmental friendliness, is the important method substituting and expand traditional organic chemical synthesis in process of sustainable development.Wherein chiral separation and asymmetric synthesis are the Application Areass of biocatalysis most magnetism.As in six large fermentoids of biological catalyst, lytic enzyme catalytic kinetics resolution of racemates can obtain quiral products, in industrial biocatalytic, play key player always.In recent years, oxydo-reductase application industrially obtained and increased rapidly.At present, the ratio adopting the industry of lytic enzyme Kinetic Resolution, biological catalysis and biological oxidation process to prepare optical activity chirality compound is 4:2:1.Biomass cells reduction method is for Kinetic Resolution, and maximum advantage is that theoretical yield can reach 100%, and Atom economy is good.But bioreduction needs the participation of coenzyme or cofactor, limit its application to a certain extent.Due to the dependent cause of coenzyme, in bioreduction, many Bian intact cells are as catalyzer, realize the purification procedures eliminating enzyme in body while coenzyme cyclic regeneration.
(S)-phenyl-(2-pyridyl) methyl alcohol is the important chiral building block of synthesis of chiral medicine, fine chemicals, agricultural chemicals product, and owing to there being 2 chiral centres, make chemosynthesis comparatively difficult, cost is high.The present invention will adopt biocatalysis to prepare (S)-phenyl-(2-pyridyl) methyl alcohol.
Summary of the invention
The present invention adopts Penicillium cell catalysis to prepare (S)-phenyl-(2-pyridyl) methyl alcohol, and reaction formula is as follows:
Substrate 2-benzoyl pyridine (1), through Penicillium notatum catalyzed reaction, obtains product (S)-phenyl-(2-pyridyl) methyl alcohol (2).Have multiple-microorganism can catalysis this reaction, through great many of experiments screening, finally determine adopt Penicillium notatum as catalyzer because its catalysis 1 reaction effect best, reaction yield, enantiomeric excess rate (ee%) are all very high.
Many Penicillium notatums can carry out this reaction of biocatalysis, but its effect is different, differs greatly, and through experiment, the present invention selects Penicillium strain to be ATCC 204049, and its this reaction effect of catalysis is best.
developing medium:
1, nutrient solution composition: corn steep liquor (with dry basis) content is 35-45g/L; yeast extract 8-10 g/L; glucose 18-22 g/L; malt extract 25-30 g, ammonium sulfate 0.5-0.7g/L, magnesium sulfate 0.3-0.4g/L; phosphoric acid dioxy potassium 1.8-2.3g/L; calcium carbonate 0.7 g/L, ferrous sulfate 0.18 g/L, manganous sulfate 0.025 g/L; PH 4.7-4.9.
2, solid medium composition: the agar powder adding 1.5-2% in liquid medium within.
Prepared by Penicillium cell.Penicillium notatum through inclined-plane, shaking flask, seed tank culture obtain seed liquor; Fermentor tank adds nutrient solution, and coefficient is 0.6-0.7, and 121 DEG C of autoclavings 30 minutes, are cooled to 25-26 DEG C, by Penicillium notatum
aTCC204049 seed liquor are seeded to fermentor tank, inoculative proportion is 10-15%, ventilation ratio is 0.5-1V/(V minute), namely per minute air flow is 0.5-1 times of fermentating liquid volume, cultivate 32-40 hour for 25-26 DEG C, wet Penicillium cell is obtained, as biocatalytic reaction catalyzer with filtering centrifuge is centrifugal.Wet cell preparation technique is mature technology.
Because substrate, product all have restraining effect to Penicillium cell, in order to reduce the suppression of substrate, products upon cell, the present invention adopts bleaching earth adsorption substrate, in reaction, when substrate is reacted by cell catalysis, when concentration reduces, substrate from carclazyte stripping, postreaction consume substrate.Meanwhile, product, by bleaching earth adsorption, decreases its concentration in water, thus considerably reduces the suppression of substrate, products upon cell.The ratio of substrate and carclazyte, determines the concentration of substrate in reaction solution, product, and concentration is also relevant with temperature of reaction, reaction solution composition.Different cell is different to the susceptibility of substrate, product, so, great many of experiments be carried out, best substrate and the ratio of carclazyte could be determined.Experiment shows, for bacterial strain of the present invention, reaction solution composition, temperature of reaction, best substrate and the ratio of carclazyte are 0.27-0.3.During concrete absorption, substrate 2-benzoyl pyridine carclazyte is absorbed, namely obtains the carclazyte having adsorbed substrate.Regulate substrate and carclazyte consumption, make the quality ratio of substrate and carclazyte be 0.27-0.3.Carclazyte used is common carclazyte, and commercially, sterilizing, Preservation in sterile condition is stand-by.
Phosphate buffered saline buffer is added in bottom ventilation stirred tank, pH is 6.6, add the carclazyte having adsorbed substrate 2-benzoyl pyridine, substrate 2-benzoyl pyridine addition is made to be 80-90 g/L, corn steep liquor (with dry basis) content is 12-15g/L, and glucose content is 15-18g/L, wood sugar 13-15g/L, tween 80 content is 12-15g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 26-27 DEG C, add wet Penicillium cell and make concentration be 40-44g/L, ventilation ratio is 0.13-0.14V/(V minute) namely per minute air flow be 0.13-14 times of reaction solution volume, carry out biocatalytic reaction, the reaction times is 60-65 hour; After reaction terminates, leach cell, carclazyte respectively, be extracted with ethyl acetate reaction solution, extraction carclazyte, combined ethyl acetate extraction liquid, steam ethyl acetate, obtain product (S)-phenyl-(2-pyridyl) methyl alcohol, reaction conversion ratio 96-98%, product yield 94-96%, enantiomeric excess rate (ee%) 98-99%.
The present invention carries out the work and comprises bacterial strain selection (selecting from more or less a hundred bacterial strain), catalytic reaction condition optimizes (temperature of reaction, air flow, pH), reaction medium is selected and concentration optimization (concentration of substrate, glucose content, Xylose Content, kinds of surfactants (more than 20, kind selects 1) and concentration, other multiple components is selected to get rid of), solid absorption is adopted to control substrate product concentration, thus the suppression reduced cell, test carclazyte, diatomite, cotton gauze, ramie gauze, the many kinds of solids materials such as macropore resin, also once carried out the test of water-organic solvent 2 phase system.Because tested number is very large, although have employed response surface optimization design experiment, drastically reduce the area tested number, tested number is still very large, and total Test carries out just completing more than 2 years, reaches current technical scheme.For photolytic activity product, as reaction conversion ratio 96-98%, during product yield 94-96%, enantiomeric excess rate (ee%), still up to 98-99%, is very not easily, and our work achieves marked improvement.
embodiment 1
Wet Penicillium notatum ATCC 204049 cell is produced, as biocatalytic reaction catalyzer by ordinary method.
The carclazyte making method of having adsorbed substrate is, is absorbed by substrate 2-benzoyl pyridine carclazyte, namely obtains the carclazyte having adsorbed substrate, regulates substrate and carclazyte consumption, makes the quality ratio of substrate and carclazyte be 0.27.Carclazyte used is common carclazyte, and commercially, sterilizing, Preservation in sterile condition is stand-by, and other embodiment clay treatment is identical.
Phosphate buffered saline buffer is added in 15L bottom ventilation stirred tank, pH is 6.6, add the carclazyte having adsorbed substrate 2-benzoyl pyridine, substrate 2-benzoyl pyridine addition is made to be 80-90 g/L, corn steep liquor (with dry basis) content is 12g/L, and glucose content is 15g/L, wood sugar 13g/L, tween 80 content is 12g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 26 DEG C, add wet Penicillium cell and make concentration be 40g/L, ventilation ratio is 0.13V/(V minute) namely per minute air flow be 0.13 times of reaction solution volume, carry out biocatalytic reaction, the reaction times is 60 hours; After reaction terminates, leach cell, carclazyte respectively, be extracted with ethyl acetate reaction solution, extraction carclazyte, combined ethyl acetate extraction liquid, steam ethyl acetate, obtain product (S)-phenyl-(2-pyridyl) methyl alcohol, reaction conversion ratio 96%, product yield 94%, enantiomeric excess rate (ee%) 99%.
embodiment 2
Wet Penicillium notatum ATCC 204049 cell is produced, as biocatalytic reaction catalyzer by ordinary method.
The carclazyte making method of having adsorbed substrate is, is absorbed by substrate 2-benzoyl pyridine carclazyte, namely obtains the carclazyte having adsorbed substrate, regulates substrate and carclazyte consumption, makes the quality ratio of substrate and carclazyte be 0.3.
Phosphate buffered saline buffer is added in 100L bottom ventilation stirred tank, pH is 6.6, add the carclazyte having adsorbed substrate 2-benzoyl pyridine, substrate 2-benzoyl pyridine addition is made to be 90 g/L, corn steep liquor (with dry basis) content is 15g/L, and glucose content is 18g/L, wood sugar 15g/L, tween 80 content is 15g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 27 DEG C, add wet Penicillium cell and make concentration be 44g/L, ventilation ratio is 14V/(V minute) namely per minute air flow be 14 times of reaction solution volumes, carry out biocatalytic reaction, the reaction times is 65 hours; After reaction terminates, leach cell, carclazyte respectively, be extracted with ethyl acetate reaction solution, extraction carclazyte, combined ethyl acetate extraction liquid, steam ethyl acetate, obtain product (S)-phenyl-(2-pyridyl) methyl alcohol, reaction conversion ratio 96%, product yield 94%, enantiomeric excess rate (ee%) 99%.
embodiment 3
Wet Penicillium notatum ATCC 204049 cell is produced, as biocatalytic reaction catalyzer by ordinary method.
The carclazyte making method of having adsorbed substrate is, is absorbed by substrate 2-benzoyl pyridine carclazyte, namely obtains the carclazyte having adsorbed substrate, regulates substrate and carclazyte consumption, makes the quality ratio of substrate and carclazyte be 0.28.Carclazyte used is common carclazyte, and commercially, sterilizing, Preservation in sterile condition is stand-by, and other embodiment clay treatment is identical.
Phosphate buffered saline buffer is added in 500L bottom ventilation stirred tank, pH is 6.6, add the carclazyte having adsorbed substrate 2-benzoyl pyridine, substrate 2-benzoyl pyridine addition is made to be 80-90 g/L, corn steep liquor (with dry basis) content is 13g/L, and glucose content is 17g/L, wood sugar 14g/L, tween 80 content is 14g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 26.5 DEG C, add wet Penicillium cell and make concentration be 42g/L, ventilation ratio is 0.135V/(V minute) namely per minute air flow be 0.135 times of reaction solution volume, carry out biocatalytic reaction, the reaction times is 64 hours; After reaction terminates, leach cell, carclazyte respectively, be extracted with ethyl acetate reaction solution, extraction carclazyte, combined ethyl acetate extraction liquid, steam ethyl acetate, obtain product (S)-phenyl-(2-pyridyl) methyl alcohol, reaction conversion ratio 96-98%, product yield 94-96%, enantiomeric excess rate (ee%) 98-99%.
embodiment 4
Wet Penicillium notatum ATCC 204049 cell is produced, as biocatalytic reaction catalyzer by ordinary method.
The carclazyte making method of having adsorbed substrate is, is absorbed by substrate 2-benzoyl pyridine carclazyte, namely obtains the carclazyte having adsorbed substrate, regulates substrate and carclazyte consumption, makes the quality ratio of substrate and carclazyte be 0.3.Carclazyte used is common carclazyte, and commercially, sterilizing, Preservation in sterile condition is stand-by, and other embodiment clay treatment is identical.
Phosphate buffered saline buffer is added in 15L bottom ventilation stirred tank, pH is 6.6, add the carclazyte having adsorbed substrate 2-benzoyl pyridine, substrate 2-benzoyl pyridine addition is made to be 89 g/L, corn steep liquor (with dry basis) content is 15g/L, and glucose content is 17g/L, wood sugar 14g/L, tween 80 content is 14g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 27 DEG C, add wet Penicillium cell and make concentration be 44g/L, ventilation ratio is 0.14V/(V minute) namely per minute air flow be 0.14 times of reaction solution volume, carry out biocatalytic reaction, the reaction times is 65 hours; After reaction terminates, leach cell, carclazyte respectively, be extracted with ethyl acetate reaction solution, extraction carclazyte, combined ethyl acetate extraction liquid, steam ethyl acetate, obtain product (S)-phenyl-(2-pyridyl) methyl alcohol, reaction conversion ratio 97.5%, product yield 95.6%, enantiomeric excess rate (ee%) 98.7%.
Claims (2)
1. the method for Penicillium cell biocatalysis preparation (S)-phenyl-(2-pyridyl) methyl alcohol, it is characterized in that adding phosphate buffered saline buffer in retort, pH is 6.6, add the carclazyte having adsorbed substrate 2-benzoyl pyridine, substrate 2-benzoyl pyridine addition is made to be 80-90 g/L, corn steep liquor (with dry basis) content is 12-15g/L, glucose content is 15-18g/L, wood sugar 13-15g/L, tween 80 content is 12-15g/L, 121 DEG C of autoclavings 30 minutes; When being cooled to 26-27 DEG C, add wet Penicillium cell and make concentration be 40-44g/L, ventilation ratio is 0.13-0.14V/(V minute), carry out biocatalytic reaction, the reaction times is 60-65 hour; After reaction terminates, leach cell, carclazyte respectively, be extracted with ethyl acetate reaction solution, extraction carclazyte, combined ethyl acetate extraction liquid, steam ethyl acetate, obtain product (S)-phenyl-(2-pyridyl) methyl alcohol, reaction conversion ratio 96-98%, product yield 94-96%, enantiomeric excess rate (ee%) 98-99%.
2. method according to claim 1; it is characterized in that described carclazyte making method of having adsorbed substrate is, absorbs substrate 2-benzoyl pyridine carclazyte, namely obtains the carclazyte having adsorbed substrate; regulate substrate and carclazyte consumption, make the quality ratio of substrate and carclazyte be 0.27-0.3.
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| CN201410594403.3A CN104328154A (en) | 2014-10-30 | 2014-10-30 | Method for producing (S)-phenyl methanol from blue mold |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1028164A1 (en) * | 1999-02-12 | 2000-08-16 | Pfizer Products Inc. | Microbial conversion of 2-methylquinoxaline |
| CN1793355A (en) * | 2005-12-01 | 2006-06-28 | 华东理工大学 | Organic solvent slow-releasing system and its preparation and application for catalyzing reaction of enzyme thereof |
-
2014
- 2014-10-30 CN CN201410594403.3A patent/CN104328154A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1028164A1 (en) * | 1999-02-12 | 2000-08-16 | Pfizer Products Inc. | Microbial conversion of 2-methylquinoxaline |
| CN1793355A (en) * | 2005-12-01 | 2006-06-28 | 华东理工大学 | Organic solvent slow-releasing system and its preparation and application for catalyzing reaction of enzyme thereof |
Non-Patent Citations (1)
| Title |
|---|
| MOHAN PAL ET AL.: "Bioreduction of methyl heteroaryl and aryl heteroaryl ketones in high enantiomeric excess with newly isolated fungal strains", 《BIORESOURCE TECHNOLOGY》 * |
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Application publication date: 20150204 |