CN104313096A

CN104313096A - Method for preparing beluga swimming bladder protein peptide

Info

Publication number: CN104313096A
Application number: CN201410568674.1A
Authority: CN
Inventors: 宋林霞
Original assignee: Harbin Paitena Biotechnology Development Co Ltd
Current assignee: Harbin Paitena Biotechnology Development Co Ltd
Priority date: 2014-10-23
Filing date: 2014-10-23
Publication date: 2015-01-28

Abstract

The invention provides a method for preparing a beluga swimming bladder protein peptide. The method specifically comprises the following steps: treating and shredding fresh beluga swimming bladders, adding 5-fold dilute alkali liquor, soaking for 3 hours, performing centrifugal separation, washing the precipitate until the pH value is neutral; adding a 5-fold dilute acid solution, soaking for 2 hours, performing centrifugal separation, and washing the precipitate until the pH value is neutral; adding 10-fold water, extracting at the temperature of 90 DEG C for 6 hours, and filtering; cooling to 50 DEG C, regulating the pH value to 9, adding 0.5 percent of alkaline protease, performing enzymolysis at the temperature of 50 DEG C for 4 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; cooling to 45 DEG C, regulating the pH value to be 2.5, adding 0.3 percent of acid proteinase, performing enzymolysis at the temperature of 45 DEG C for 5 hours, and performing enzyme deactivation at the temperature of 95 DEG C for 5 minutes; and regulating the pH value to be neutral, filtering, desalting, concentrating, and drying, thereby obtaining the beluga swimming bladder protein peptide.

Description

A kind of preparation method of sturgeon fish maw protein peptide

Technical field

The present invention relates to a kind of working method of protein, specifically a kind of preparation method of sturgeon fish maw protein peptide.

Background technology

Sturgeon belongs to large-scale predacious fish, now exists only among China and Muscovite Jie Jiang Heilungkiang.Sturgeon body is huge, is larger fish, is described as " kings of fresh-water fishes ".The whole body of sturgeon is all precious, and the thick thorn of its meat is few, delicious and fresh.Sturgeon air bladder is comparatively large, and with 2 bands that hang down, accounts for 5% of sturgeon weight, containing abundant collagen protein, but it is strong but pliable in texture, not easily tear, should not directly eat, therefore, well do not utilized, needing to carry out processing could be better digested.

Summary of the invention

The invention provides a kind of preparation method of sturgeon fish maw protein bioactive peptide.

The object of the invention is to be realized by following manner: fresh sturgeon air bladder, cut open, removing blood vessel and mucous membrane, flatten, clear water is rinsed well, drain, chopping, adds 0.5% aqueous sodium carbonate of 5 times, soaks 3h, the centrifugal 10min of 3500rpm, throw out is neutral to pH with clear water repetitive scrubbing; Add 0.2% aqueous hydrochloric acid of 5 times, soak the centrifugal 10min of 2h, 3500rpm, throw out is neutral to pH with clear water repetitive scrubbing; Add the pure water of 10 times, be heated to 90 DEG C, under constant temperature stirs, extracting 6h, filters; Filtrate is cooled to 50 DEG C, adjusts pH to 9, add Sumizyme MP 0.5%, 50 DEG C of enzymolysis 4h, 95 DEG C of enzyme 5min that go out; Be cooled to 45 DEG C, adjust pH to 2.5, add aspartic protease 0.3%, 45 DEG C of enzymolysis 5h, 95 DEG C of enzyme 5min that go out, adjust pH to neutral, filter, by filtrate desalination, vacuum concentration, spraying dry, obtain sturgeon fish maw protein bioactive peptide.

Protein peptide has higher biological activity and nutritive value than the amino acid of equivalent, small-molecule peptide and amino acid almost all can be absorbed and used by the Epithelium of intestinal villus cell of animal completely, enter into blood, then each position of human body is transported to, protein just can be absorbed and used after then having to pass through digestion, therefore the speed that is absorbed by the body of peptide class is faster than the total free aminoacids of equivalent, greatly faster than the protein of equivalent.

The present invention is with sturgeon air bladder for raw material, by the method for enzymolysis, the protein transduction in not easily directly edible sturgeon air bladder is turned to small molecules, protein peptide soluble in water.The protein contained due to sturgeon air bladder is based on collagen protein, and therefore, sturgeon fish maw protein peptide belongs to collagen peptide, has beauty treatment, anti-oxidant, anti-ageing function of waiting for a long time, may be used for develop dietetic foodstuffs, protective foods etc.

Four, specific embodiment

Embodiment 1

Get fresh sturgeon air bladder, cut open, removing blood vessel and mucous membrane, flatten, clear water is rinsed well, drains, chopping; Take the air bladder 1kg of chopping, adding 5 L concentration is the aqueous sodium carbonate of 0.5%, soaks the centrifugal 10min of 3h, 3500rpm, and throw out is neutral to pH with clear water repetitive scrubbing; Add the aqueous hydrochloric acid that 5L concentration is 0.2%, soak the centrifugal 10min of 2h, 3500rpm, throw out is neutral to pH with clear water repetitive scrubbing; Add 10L pure water, be heated to 90 DEG C, under constant temperature stirs, extracting 6h, filters; Filtrate is cooled to 50 DEG C, adjusts pH to 9, add Sumizyme MP 50g, 50 DEG C of enzymolysis 4h, go out at 95 DEG C enzyme 5min, is cooled to 45 DEG C, adjust pH to 2.5, add aspartic protease 30g, 45 DEG C of enzymolysis 5h, go out enzyme 5min at 95 DEG C, adjust pH to neutral, filter, by filtrate desalination, vacuum concentration, spraying dry, obtain sturgeon air bladder collagen active peptide.

Embodiment 2

Get fresh sturgeon air bladder, cut open, removing blood vessel and mucous membrane, flatten, clear water is rinsed well, drains, chopping; Take the air bladder 500g of chopping, add the aqueous sodium carbonate that 2500mL concentration is 0.5%, soak the centrifugal 10min of 3h, 3500rpm, throw out is neutral to pH with clear water repetitive scrubbing; Add the aqueous hydrochloric acid that 2500mL concentration is 0.2%, soak the centrifugal 10min of 2h, 3500rpm, throw out is neutral to pH with clear water repetitive scrubbing; Add 5000mL pure water, be heated to 90 DEG C, under constant temperature stirs, extracting 6h, filters; Filtrate is cooled to 50 DEG C, adjusts pH to 9, add Sumizyme MP 25g, 50 DEG C of enzymolysis 4h, go out at 95 DEG C enzyme 5min, is cooled to 45 DEG C, adjust pH to 2.5, add aspartic protease 15g, 45 DEG C of enzymolysis 5h, go out enzyme 5min at 95 DEG C, adjust pH to neutral, filter, by filtrate desalination, vacuum concentration, spraying dry, obtain sturgeon air bladder collagen active peptide.

Claims

1. the invention provides a kind of preparation method of sturgeon fish maw protein bioactive peptide, its feature is as follows: fresh sturgeon air bladder, cuts open, removing blood vessel and mucous membrane, flatten, clear water is rinsed well, drains, chopping, add 0.5% aqueous sodium carbonate of 5 times, soak the centrifugal 10min of 3h, 3500rpm, throw out is neutral to pH with clear water repetitive scrubbing; Add 0.2% aqueous hydrochloric acid of 5 times, soak the centrifugal 10min of 2h, 3500rpm, throw out is neutral to pH with clear water repetitive scrubbing; Add the pure water of 10 times, be heated to 90 DEG C, under constant temperature stirs, extracting 6h, filters; Filtrate is cooled to 50 DEG C, adjusts pH to 9, add Sumizyme MP 0.5%, 50 DEG C of enzymolysis 4h, 95 DEG C of enzyme 5min that go out; Be cooled to 45 DEG C, adjust pH to 2.5, add aspartic protease 0.3%, 45 DEG C of enzymolysis 5h, 95 DEG C of enzyme 5min that go out, adjust pH to neutral, filter, by filtrate desalination, vacuum concentration, spraying dry, obtain sturgeon fish maw protein bioactive peptide.