CN107034258A - A kind of insect protein extracting method - Google Patents
A kind of insect protein extracting method Download PDFInfo
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- CN107034258A CN107034258A CN201710230757.3A CN201710230757A CN107034258A CN 107034258 A CN107034258 A CN 107034258A CN 201710230757 A CN201710230757 A CN 201710230757A CN 107034258 A CN107034258 A CN 107034258A
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- insect
- extracting method
- insect protein
- extraction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/303—Extraction; Separation; Purification by precipitation by salting out
Abstract
The present invention relates to a kind of insect protein extracting method, it is to pass through insect body to pre-process, dry and then blend worm slurry is made, and degreasing filtering is using Enzymatic Extraction, purifies and separates are dry, obtained insect protein to worm slurry solution.Extraction conditions of the present invention are gentleer, and extraction time is shorter, can more retaining protein nutritive value, while under conditions of Lower degrees of hydrolysis, also having certain improvement to the functional character of yellow meal worm albumen, environmental pollution is smaller.
Description
Technical field
The present invention relates to a kind of protein extracting method, specifically a kind of insect protein extracting method.
Background technology
Insect in the world there are about more than 100 ten thousand kinds, have more than 3650 to plant edible.Predicted according to brainstrust, to 21 century, elder brother
Worm will originate as the third-largest proteinoid for being only second to microorganism and cell biological, because caste is more, quantity is big, distribution
Extensively, breed fast, high protein, low fat, low cholesterol, rationally, fleshy fiber is few for trophic structure, is easy to absorb again, advantage is dashed forward
Go out, and be that countries in the world are of interest better than phytoprotein.
The carnivorism custom of China, it is with a long history, it is more prevailing in minority area.Vast territory and abundant resources for China, insect resources kind
Class is especially enriched.In recent years, once there is the product foreign exchange earning of locust, cicada.In the new resources exploitation seminar of following food, I
Ant is formally classified as new resources exploration project by the Ministry of Public Health of state.China also proposes to set up national resource insect industrial promotion committee member
Meeting, to promote the development and utilization of insect food and medicine.1994 years, celebrating Insects in China association 50 anniversaries of establishment
When, and 1996 years in National Resources insect industrialization seminar, be all solemnly proposed magnificence insect fete, expand significantly
The big edible influence of insect.Due to exploitation insect food and medicine, it can change harmful to treasure, achieve many things at one stroke, it is 21 century, emerging
One upsurge for establishing insect food industry is played, corresponding insect food and medicine will continuously emerge.
The leading food of 21 century is functional food, and the exactly typical functional food of insect food.In view of insect
It is the huge living resources of a major class development potentiality and nutrition treasure-house, energy medicine can be eaten, sportsman, the elderly, teenager can be used as
The senior nutrition of children and effective medicine.
Biological high-tech product --- insect protein Southern Yangtze University Foodstuffs Academy doctoral advisor Xia Wenshui is taught, group
Knit scientist in terms of state key subject Southern Yangtze University, 28 immunologys of Ge Rui biological studies mechanism, clinical medicine, science of heredity
Research cooperation, finally successfully develops albumen of new generation using biological enzymolysis patented technology from insect！Now, domestic-developed elder brother
Worm food, just as being a dark horse, sweeps across China.By years of researches, insect nutrient protein, insect active are largely extracted
Albumen, insect chitin（That is chitin）, and work out insect antimicrobial peptide, anti-cancer active matter etc..
The protein content of insect will be high than beef, pork, chicken, fish.For example, dry wasp contains protein about 81%, honey
Honeybee 43%, cicada 72%, grasshopper 70%, cricket 65%, oryza chinensis 60.08%, tussah chrysalis 52.14%, fly maggot 60.88%, yellow meal worm 63.19%,
Ancient cooking vessel reality thorniness ant 64.50%, red chest thorniness ant 58.60% etc., and the various amino acid wanted in protein rich in needed by human body, especially
It is the lysine, threonine, methionine that can not voluntarily synthesize etc..In addition, also containing a small amount of fat, carbohydrate, a variety of in polypide
Vitamin and mineral matter etc..Chitin can also be extracted from insect cuticle, antibacterial peptide etc. is extracted from blood.
It is typically all alkaline process and salt method that now popular insect protein, which is extracted, and alkaline process, salt method are all present
Shortcoming.Although alkalinity extraction recovery rate is higher, extract simple and easy to apply, production cost is relatively low, under conditions of high-alkali, albumen
A bad alanine can be generated in extract solution, this material is not only poisonous, but also can cause the loss of nutriment.Meanwhile, it is high
It can also cause protein to hydrolyze under the conditions of alkali, accelerate Maillard reaction, dark brown color substance be produced, so as to cause extract solution color
Deepen, nonprotein content increase in extract, separating effect reduction.In addition, alkaline process and salt method are to consersion unit
Requirement it is higher, can cause serious environmental pollution, increase the production cost of post processing.
The content of the invention
It is an object of the invention to provide a kind of insect protein extracting method, to solve what is proposed in above-mentioned background technology
Problem.
To achieve the above object, the present invention provides following technical scheme：
A kind of above-mentioned insect protein extracting method, is to pass through insect body to pre-process, dry and then blend worm slurry is made,
Degreasing is filtered, and is using Enzymatic Extraction to worm slurry solution, purifies and separates are dried, and insect protein is made.
A kind of insect protein extracting method, specifically includes following steps：
(1) by insect body by removal of impurities, clean, sterilizing, drying；
(2) insect body Jing Guo step (1) is pulverized and sieved；
(3) worm is made to the crushed material by step (2) using organic solvent to starch, then is soaked；
(4) supercritical fluid CO is passed through2Extraction is to step（3）Worm slurry carry out extraction degreasing；
(5) by step（4）The degreasing thing of middle generation adds water homogenate；
(6) by step（5）Elimination cuticular substance is crossed in the homogenate of middle generation, is homogenized under conditions of 100 °, extracts 20-30min；
（7）By step（6）In the homogenate enzyme-added hydrolysis of 1-1.5% by volume, solid-liquid ratio is 1:10, temperature is 50-55 °, pH value
7.5-8, constant temperature 3-5 hours；
(8) step will be passed through（7）Slurries use 3% activated carbon decolorizing, adsorption temp be 30 °, the time be 2 hours；
（9）Step will be passed through（8）Product electricity consumption point-combination method of saltouing carry out enrichment precipitation；
(10) by step（9）The centrifuge desalination of the sediment of middle generation；
(11) by step（10）The desalination product of middle generation adds water homogenate；
（12）By step（11）The homogenate sedimentation and drawing of middle generation；
(13) by step（12）The sediment of middle generation removes insoluble protein with small molecule bioactive peptide isolation technics；
(14) by step（13）The product utilization spray drying process of generation can be prepared by insect protein.
The insect protein extracting method, wherein：The step（7）In enzyme be trypsase and chymotrypsin
Combination.
The insect protein extracting method, wherein, the step（4）In supercritical fluid CO2The technique of extraction
Condition is：Extracting pressure 30MPa, CO2Flow 20L/h, 35 ° of temperature, time 2.5h.
The insect protein extracting method, wherein：The step（7）In temperature be 55 °.
The insect protein extracting method, wherein：PH value in the step (7) is 8.
The insect protein extracting method, wherein：The constant temperature time of the pH value 7.5-8 is 5h.
Beneficial effect：
Compared with prior art, extraction conditions of the present invention are gentleer, and extraction time is shorter, can more retaining protein battalion
Value is supported, while under conditions of Lower degrees of hydrolysis, also having certain improvement, environmental pollution to the functional character of yellow meal worm albumen
It is smaller.
Brief description of the drawings
Fig. 1 extracts flow chart for the present invention；
Fig. 2 is supercritical fluid CO2Each condition orthogonal experiment form of extraction；
Fig. 3 is Fig. 2 process conditions factor level tables；
Fig. 4 is the structural representation of the double enzyme composite test lists of Enzymatic Extraction；
Fig. 5 is double enzyme composite test block diagrams；
The protein of Fig. 6 distinct methods separates out experiment block diagram；
Fig. 7 is that various drying means contrast list.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Refer in Fig. 1~4, the embodiment of the present invention, a kind of insect protein extracting method, be by pre- by insect body
Handle, dry and then blend and worm slurry is made, degreasing filtering is using Enzymatic Extraction, purifies and separates are dried, system to worm slurry solution
Obtain insect protein.
Insect protein extracting method of the present invention, is comprised the following steps that：
(1) by insect body by removal of impurities, clean, sterilizing, drying；
(2) step will be passed through（1）Insect body pulverize and sieve；
(3) using organic solvent to passing through step（2）Crushed material worm slurry is made, then soaked；
(4) supercritical fluid CO is passed through2Extraction is to step（3）Worm slurry carry out extraction degreasing；
(5) by step（4）The degreasing thing of middle generation adds water homogenate；
(6) by step（5）Elimination cuticular substance is crossed in the homogenate of middle generation, is homogenized under conditions of 100 °, extracts 20-30min；
(7) by step（6）In the homogenate enzyme-added hydrolysis of 1-1.5% by volume, solid-liquid ratio is 1:10, temperature is 50-55 °, pH value
7.5-8, constant temperature 3-5 hours；
(8) step will be passed through（7）Slurries use 3% activated carbon decolorizing, adsorption temp be 30 °, the time be 2 hours；
(9) step will be passed through（8）Product electricity consumption point-combination method of saltouing carry out enrichment precipitation；
(10) by step（9）The centrifuge desalination of the sediment of middle generation；
(11) by step（10）The desalination product of middle generation adds water homogenate；
(12) by step（11）The homogenate sedimentation and drawing of middle generation；
(13) by step（12）The sediment of middle generation removes insoluble protein with small molecule bioactive peptide isolation technics；
(14) by step（13）The product utilization spray drying process of generation can be prepared by insect protein.
It is used as further scheme of the invention：Above-mentioned steps（7）In enzyme for trypsase and chymotrypsin group
Close.
It is used as further scheme of the invention：Above-mentioned steps（4）In supercritical fluid CO2The process conditions of extraction
For：Extracting pressure 30MPa, CO2Flow 20L/h, 35 ° of temperature, time 2.5h.
It is used as further scheme of the invention：Above-mentioned steps（7）In temperature be 55 °.
It is used as further scheme of the invention：Above-mentioned steps（7）In pH value be 8.
It is used as further scheme of the invention：Above-mentioned steps（7）Middle pH value 7.5-8 constant temperature time is 5h.
With reference to specific embodiment, the invention will be further described：
Embodiment 1（Extract yellow meal worm albumen）
Comprise the following steps that：
(1) by yellow meal worm polypide by removal of impurities, clean, sterilizing, drying；
（2）Step will be passed through（1）The broken sieving of flour weevil body powder；
(3) using organic solvent to passing through step（2）Crushed material worm slurry is made, then soaked；
(4) it is by form optimum process condition as shown in Figure 2：Extracting pressure 30MPa, CO2Flow 20L/h, 35 ° of temperature,
Time 2.5h carries out supercritical fluid CO2Extraction is to step（3）Worm slurry carry out extraction degreasing；
Following table is the supercritical fluid CO that traditional organic solvent extractionprocess and the present invention are used2The contrast of extraction：
(5) by step（4）The degreasing thing of middle generation adds water homogenate；
(6) by step（5）Elimination cuticular substance is crossed in the homogenate of middle generation, is homogenized under conditions of 100 °, extracts 30min；
(7) table figure is tested by Fig. 4 and Fig. 5, by step（6）In homogenate pass through by volume 1.5% plus double enzymes combine enzymes
（The combination of 0.5% trypsase and 0.5% chymotrypsin）Hydrolysis, solid-liquid ratio is 1:10, temperature is 55 °, pH value 8, constant temperature
3-5 hours；
(8) step will be passed through（7）Slurries use 3% activated carbon decolorizing, adsorption temp be 30 °, the time be 2 hours；
(9) experiment is separated out by protein and Fig. 6 understands that step will be passed through（8）Product electricity consumption point-combination method of saltouing carry out
Enrichment precipitation, protein eduction rate highest；
(10) by step（9）The centrifuge desalination of the sediment of middle generation；
(11) the desalination product of generation in step (10) is added water homogenate；
(12) by the homogenate sedimentation and drawing of generation in step (11)；
(13) sediment of generation in step (12) is removed into insoluble protein with small molecule bioactive peptide isolation technics；
(14) by experiment and test structure tabular drawing 7, so by step by spray drying process drying effect most preferably
(13) the product utilization spray drying process of generation can be prepared by insect protein.
Extraction conditions of the present invention are gentleer, and extraction time is shorter, can more retaining protein nutritive value, simultaneously
Under conditions of Lower degrees of hydrolysis, also there is certain improvement to the functional character of yellow meal worm albumen, environmental pollution is smaller.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as to the claim involved by limitation.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It may be appreciated other embodiment.
Claims (6)
1. a kind of insect protein extracting method, it is characterised in that be by pre-processing, drying and then blend system by insect body
Adult is starched, degreasing filtering, is using Enzymatic Extraction to worm slurry solution, purifies and separates are dried, and insect protein is made.
2. a kind of insect protein extracting method, it is characterised in that specifically include following steps：
(1) by insect body by removal of impurities, clean, sterilizing, drying；
(2) insect body Jing Guo step (1) is pulverized and sieved；
(3) worm is made to the crushed material by step (2) using organic solvent to starch, then is soaked；
(4) supercritical fluid CO is passed through2Extraction is to step（3）Worm slurry carry out extraction degreasing；
(5) by step（4）The degreasing thing of middle generation adds water homogenate；
(6) by step（5）Elimination cuticular substance is crossed in the homogenate of middle generation, is homogenized under conditions of 100 °, extracts 20-30min；
（7）By step（6）In the homogenate enzyme-added hydrolysis of 1-1.5% by volume, solid-liquid ratio is 1:10, temperature is 50-55 °, pH value
7.5-8, constant temperature 3-5 hours；
(8) step will be passed through（7）Slurries use 3% activated carbon decolorizing, adsorption temp be 30 °, the time be 2 hours；
（9）Step will be passed through（8）Product electricity consumption point-combination method of saltouing carry out enrichment precipitation；
(10) by step（9）The centrifuge desalination of the sediment of middle generation；
(11) by step（10）The desalination product of middle generation adds water homogenate；
（12）By step（11）The homogenate sedimentation and drawing of middle generation；
(13) by step（12）The sediment of middle generation removes insoluble protein with small molecule bioactive peptide isolation technics；
(14) by step（13）The product utilization spray drying process of generation can be prepared by insect protein.
3. insect protein extracting method as claimed in claim 2, it is characterised in that：The step（7）In enzyme be pancreas egg
The combination of white enzyme and chymotrypsin；
Insect protein extracting method as claimed in claim 2, it is characterised in that the step（4）In supercritical fluid
CO2The process conditions of extraction are：Extracting pressure 30MPa, CO2Flow 20L/h, 35 ° of temperature, time 2.5h.
4. insect protein extracting method as claimed in claim 2, it is characterised in that：The step（7）In temperature be
55°。
5. insect protein extracting method as claimed in claim 2, it is characterised in that：PH value in the step (7) is 8.
6. insect protein extracting method as claimed in claim 2, it is characterised in that：The constant temperature time of the pH value 7.5-8
For 5h.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107889930A (en) * | 2017-11-28 | 2018-04-10 | 湖北聚注通用技术研究有限公司 | A kind of preparation method and applications of firefly pupa protein hydrolyzate |
| CN108236040A (en) * | 2018-01-05 | 2018-07-03 | 佛山汇沐化学科技有限公司 | A kind of extracting method of mole cricket albumen and its application |
| EP2863762B1 (en) | 2012-06-21 | 2018-08-08 | Buhler (Changzhou) Insect Technologies Co.Ltd. | Method to convert insects or worms into nutrient streams and compositions obtained thereby |
| CN111000021A (en) * | 2019-10-21 | 2020-04-14 | 长沙理工大学 | Production equipment and method of insect protein powder |
| CN111213886A (en) * | 2020-01-09 | 2020-06-02 | 菏泽佳诺佳宠物用品有限公司 | Insect peptide powder and peptide pulp production process |
| CN115413729A (en) * | 2022-09-06 | 2022-12-02 | 昆明理工大学 | Preparation method and application of insect protein |
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| CN101353684A (en) * | 2008-08-22 | 2009-01-28 | 中南林业科技大学 | Technology for extracting protein in pine caterpillar using alkali proteinase method |
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| CN101353684A (en) * | 2008-08-22 | 2009-01-28 | 中南林业科技大学 | Technology for extracting protein in pine caterpillar using alkali proteinase method |
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| 罗赞等: "黄粉虫蛋白质酶法提取的研究", 《工艺探讨》 * |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2863762B1 (en) | 2012-06-21 | 2018-08-08 | Buhler (Changzhou) Insect Technologies Co.Ltd. | Method to convert insects or worms into nutrient streams and compositions obtained thereby |
| EP3398444B1 (en) | 2012-06-21 | 2021-03-03 | Bühler Insect Technology Solutions AG | Method to convert insects or worms into nutrient streams and compositions obtained thereby |
| CN107889930A (en) * | 2017-11-28 | 2018-04-10 | 湖北聚注通用技术研究有限公司 | A kind of preparation method and applications of firefly pupa protein hydrolyzate |
| CN108236040A (en) * | 2018-01-05 | 2018-07-03 | 佛山汇沐化学科技有限公司 | A kind of extracting method of mole cricket albumen and its application |
| CN111000021A (en) * | 2019-10-21 | 2020-04-14 | 长沙理工大学 | Production equipment and method of insect protein powder |
| CN111213886A (en) * | 2020-01-09 | 2020-06-02 | 菏泽佳诺佳宠物用品有限公司 | Insect peptide powder and peptide pulp production process |
| CN115413729A (en) * | 2022-09-06 | 2022-12-02 | 昆明理工大学 | Preparation method and application of insect protein |
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