CN104306629B - A kind of Chinese medicine composition of chicken colibacillosis resisting disease and preparation method thereof - Google Patents
A kind of Chinese medicine composition of chicken colibacillosis resisting disease and preparation method thereof Download PDFInfo
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/708—Rheum (rhubarb)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/89—Cyperaceae (Sedge family)
- A61K36/8905—Cyperus (flatsedge)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1617—Organic compounds, e.g. phospholipids, fats
- A61K9/1623—Sugars or sugar alcohols, e.g. lactose; Derivatives thereof; Homeopathic globules
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
Chinese medicine composition the present invention provides a kind of chicken colibacillosis resisting disease and preparation method thereof, the composition is made of rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis, 5 30 parts of rheum officinale, 5 30 parts of rhizoma cyperi, 5 30 parts of radix astragali, 5 30 parts of Herba Andrographitis based on its parts by weight, in prescription ratio by the Chinese medicine is extracted, medicinal extract is made in refined, concentration technology to obtain the final product；For the Chinese medicine composition by traditional Chinese medicine reasonable formula, the effect of antibacterial anti-inflammatory, is extremely notable, and a kind of effective medicine of chicken colibacillosis resisting disease is provided for poultry husbandry, has the characteristics that quick, curative effect really, no drug residue, without any side effects；Its preparation method is simple, is adapted to the needs of scale industrial production.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition and preparation method thereof, especially a kind of Chinese medicine group of chicken colibacillosis resisting disease
Compound and preparation method thereof.
Background technology
Chicken colibacillosis is as the infectious disease caused by the Enteropathogenic Escherichia coli of some serotypes.The chicken of each age in days
It can fall ill, it is placed in the middle with chick and middle chicken.Clinical manifestation is a variety of disease types, it is more typical have septicemia, granuloma, pericarditis,
Perihepatitis, air bag inflammation, enteritis, omphalitis, panophthalmitis, yolk peritonitis, swollen head sydrome etc., incidence 11-69%, causes
Dead rate be 40.2-90.3%, and during with other cause of disease mixed infections, the death rate understands higher, its harm is occupied first of bacteriosis, often
The loss of tremendous economic is brought to poultry husbandry.
But the prevention for chicken colibacillosis still suffers from problems at present, first, vaccine immunization is many because of its serotype
More, currently available vaccines, existing vaccines cannot be prevented effectively；Second, although antibacterials are still to prevent the sick main means, but due to anti-medicine
The emergence of bacterial strain, many antibacterials curative effects decline；Third, the medicament residue caused by largely using antimicrobial is so as to trigger
Food-safety problem.
Therefore, using the Chinese herbal medicine resource of China's abundant, seek a kind of antibacterial action is strong, residual is low with toxicity, to poultry
The Chinese medicine composition for being not likely to produce drug resistance is the active demand in market.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of Chinese medicine composition of chicken colibacillosis resisting disease.
Another technical problem to be solved by this invention is the Chinese medicine composition for providing above-mentioned chicken colibacillosis resisting disease
Preparation method.
In order to solve the above technical problems, the technical scheme is that：
A kind of Chinese medicine composition of chicken colibacillosis resisting disease, is made of rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis, by its weight
5-30 parts of number meter rheum officinale, 5-30 parts of rhizoma cyperi, 5-30 parts of radix astragali, 5-30 parts of Herba Andrographitis.
Preferably, the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease is big based on its parts by weight based on its parts by weight
10-20 parts yellow, 10-20 parts of rhizoma cyperi, 10-20 parts of radix astragali, 10-20 parts of Herba Andrographitis.
Preferably, the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, 10 parts of rheum officinale based on its parts by weight, rhizoma cyperi 20
Part, 10 parts of radix astragali, 20 parts of Herba Andrographitis.
The preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, in prescription ratio by the Chinese medicine it is extracted,
Medicinal extract is made in refined, concentration technology.
Preferably, the preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, specific preparation process are as follows：
(1) it is raw material to weigh rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis in prescription ratio, is uniformly mixed；
(2) medicinal material 4-10 times of water measured of gross weight is added into above-mentioned four tastes Chinese medicine, is decocted 2 times, decocts 1.5- for the first time
2.0 it is small when, second decoct 1.0-1.5 it is small when, collecting decoction, filtration, filtrate stand 12-36 it is small when, take supernatant to carry out dense
Contracting, adds ethanol to alcohol content 60-70% (v/v), places, filtration；
(3) relative density 1.35-1.4 is concentrated the filtrate to, and recycles ethanol, that is, obtains medicinal extract.
Preferably, the preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, excipient system is added into medicinal extract
Into particle.
Preferably, the preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, the excipient for Icing Sugar and/or
Dextrin.
Preferably, the preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, Icing Sugar and paste are added into medicinal extract
Particle is refined into, wherein, the mass ratio of medicinal extract and Icing Sugar, dextrin is 1:2-3:2-3.
Preferably, the preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, the particle is in 60-70 DEG C of condition
Lower drying, crosses 10-30 mesh sieves twice in succession.
Using the Chinese medicine composition of chicken colibacillosis resisting disease made of above-mentioned preparation method, when chicken colibacillosis resisting is sick,
Medicine is made into liquid according to 1.0g/L, whole day amount of drinking water is calculated according to chicken age in days, is then poured out further according to whole day amount of drinking water
The amount of medicine used, concentrates disposably drunk for diseased chicken after mixing, and 3 days as a treatment course are used in conjunction.
The beneficial effects of the invention are as follows：
The Chinese medicine composition of chicken colibacillosis resisting disease of the present invention, by traditional Chinese medicine reasonable formula, the work(of antibacterial anti-inflammatory
Effect it is extremely notable, provide a kind of effective medicine of chicken colibacillosis resisting disease for poultry husbandry, with it is quick, curative effect is true
Reality, no drug residue, it is without any side effects the characteristics of；Its preparation method is simple, is adapted to the needs of scale industrial production.
Embodiment
Technical solution of the present invention is further described with reference to specific embodiment.
Embodiment 1
A kind of Chinese medicine composition of chicken colibacillosis resisting disease, is made of rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis, and each component is used
Amount is shown in Table 1.
Table 1
| Numbering | Prescription and dosage |
| Group 1 | Rheum officinale 20kg, rhizoma cyperi 20kg, radix astragali 10kg, Herba Andrographitis 10kg |
| Group 2 | Rheum officinale 20kg, rhizoma cyperi 10kg, radix astragali 10kg, Herba Andrographitis 10kg |
| Group 3 | Rheum officinale 10kg, rhizoma cyperi 20kg, radix astragali 10kg, Herba Andrographitis 20kg |
| Group 4 | Rheum officinale 10kg, rhizoma cyperi 20kg, radix astragali 10kg, Herba Andrographitis 10kg |
| Group 5 | Rheum officinale 10kg, rhizoma cyperi 10kg, radix astragali 20kg, Herba Andrographitis 20kg |
| Group 6 | Rheum officinale 10kg, rhizoma cyperi 10kg, radix astragali 20kg, Herba Andrographitis 10kg |
| Group 7 | Rheum officinale 10kg, rhizoma cyperi 10kg, radix astragali 10kg, Herba Andrographitis 20kg |
| Group 8 | Rheum officinale 20kg, rhizoma cyperi 20kg, radix astragali 10kg, Herba Andrographitis 20kg |
The preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, specific preparation process are as follows：
(1) it is raw material to weigh rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis by above-mentioned dosage, is uniformly mixed；
(2) water of 8 times of amounts of medicinal material gross weight is added into above-mentioned four tastes Chinese medicine, is decocted 2 times, when decoction 1.8 is small for the first time,
Second decoct 1.3 it is small when, collecting decoction, filtration, filtrate stand 24 it is small when, take supernatant to be concentrated, add ethanol to containing
Alcohol amount 65% (v/v), is placed, filtration；
(3) relative density 1.35 is concentrated the filtrate to, and recycles ethanol, that is, obtains medicinal extract.
Embodiment 2
A kind of Chinese medicine composition of chicken colibacillosis resisting disease, is made of rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis, weighs rheum officinale
30kg, rhizoma cyperi 5kg, radix astragali 5kg, Herba Andrographitis 30kg.
The preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, specific preparation process are as follows：
(1) it is raw material to weigh rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis by above-mentioned dosage, is uniformly mixed；
(2) water of 10 times of amounts of medicinal material gross weight is added into above-mentioned four tastes Chinese medicine, is decocted 2 times, it is small to decoct 2.0 for the first time
When, when second of decoction 1.0 is small, collecting decoction, filtration, when filtrate standing 12-36 is small, takes supernatant to be concentrated, adds second
Alcohol is placed, filtration to alcohol content 70% (v/v)；
(3) relative density 1.4 is concentrated the filtrate to, and recycles ethanol, obtains medicinal extract；
(4) add Icing Sugar into medicinal extract and particle is made in dextrin, wherein, the mass ratio of medicinal extract and Icing Sugar, dextrin is 1:3:
2；
(5) gained particle is dried under the conditions of 70 DEG C, crosses 30 mesh sieves twice in succession, to obtain the final product.
Embodiment 3
A kind of Chinese medicine composition of chicken colibacillosis resisting disease, is made of rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis, weighs rheum officinale
5kg, rhizoma cyperi 30kg, radix astragali 30kg, Herba Andrographitis 5kg.
The preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, specific preparation process are as follows：
(1) it is raw material to weigh rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis by above-mentioned dosage, is uniformly mixed；
(2) water of 4 times of amounts of medicinal material gross weight is added into above-mentioned four tastes Chinese medicine, is decocted 2 times, when decoction 1.5 is small for the first time,
When second of decoction 1.5 is small, collecting decoction, filtration, when filtrate standing 12-36 is small, takes supernatant to be concentrated, adds ethanol extremely
Alcohol content 60% (v/v), is placed, filtration；
(3) relative density 1.38 is concentrated the filtrate to, and recycles ethanol, that is, obtains medicinal extract.
Embodiment 4
A kind of Chinese medicine composition of chicken colibacillosis resisting disease, is made of rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis, weighs rheum officinale
15kg, rhizoma cyperi 15kg, radix astragali 12kg, Herba Andrographitis 17kg.
The preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, specific preparation process are as follows：
(1) it is raw material to weigh rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis by above-mentioned dosage, is uniformly mixed；
(2) water of 6 times of amounts of medicinal material gross weight is added into above-mentioned four tastes Chinese medicine, is decocted 2 times, when decoction 1.7 is small for the first time,
Second decoct 1.2 it is small when, collecting decoction, filtration, filtrate stand 30 it is small when, take supernatant to be concentrated, add ethanol to containing
Alcohol amount 65% (v/v), is placed, filtration；
(3) relative density 1.37 is concentrated the filtrate to, and recycles ethanol, that is, obtains medicinal extract.
Embodiment 5
A kind of Chinese medicine composition of chicken colibacillosis resisting disease, is made of rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis, weighs rheum officinale
17kg, rhizoma cyperi 12kg, radix astragali 16kg, Herba Andrographitis 13kg.
The preparation method of the Chinese medicine composition of above-mentioned chicken colibacillosis resisting disease, specific preparation process are as follows：
(1) it is raw material to weigh rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis by above-mentioned dosage, is uniformly mixed；
(2) water of 7 times of amounts of medicinal material gross weight is added into above-mentioned four tastes Chinese medicine, is decocted 2 times, when decoction 1.8 is small for the first time,
Second decoct 1.4 it is small when, collecting decoction, filtration, filtrate stand 18 it is small when, take supernatant to be concentrated, add ethanol to containing
Alcohol amount 70% (v/v), is placed, filtration；
(3) relative density 1.4 is concentrated the filtrate to, and recycles ethanol, obtains medicinal extract；
(4) add Icing Sugar into medicinal extract and particle is made in dextrin, wherein, the mass ratio of medicinal extract and Icing Sugar, dextrin is 1:2:
3；
(5) gained particle is dried under the conditions of 60 DEG C, crosses 10 mesh sieves twice in succession, to obtain the final product.
Each component described in above-described embodiment 1-5 is commercially available medicinal material (being purchased from Xi'an Chinese medicine wholesale market), and is met
《Chinese veterinary pharmacopoeia》In relevant regulations.Each component is increased or decreased according to same ratio, can realize that the present invention waits to solve
The technical problem of chicken colibacillosis resisting disease certainly.
In above-mentioned Chinese medicine composition, rheum officinale has effects that heat and toxic materials clearing away, clearing heat-fire, removing pattogenic heat from the blood and toxic material from the body, dispelling stasis of blood and stimulating the menstrual flow；It is fragrant
The effect of having attached dispersing stagnated hepatoqi, menstruction regulating and pain relieving；Radix astragali has effects that invigorating qi for strengthening superficies, diuretic and maintain drug, apocenosis, expelling pus and promoting granulation；
Herba Andrographitis has the effect of clearing heat and detoxicating, cool blood, detumescence；Above all medicines share, emphasizes particularly on different fields, mutually synergistic, big to chicken
The prevention and treatment effect of enterobacteria protrudes.
Clinical test 1
Drug control group：Chinese herbal medicine is purchased from Xi'an Chinese medicine wholesale market；Ofloxacin soluble powder and Florfenicol
Powder is provided by the prosperous really Datang herding Co., Ltd in Xi'an.
Chinese medicine test group：1 gained Chinese medicine composition of embodiment, group 1-8 (being shown in Table 1).
Bacterial strain：Escherichia coli O78Purchased from China Veterinery Drug Inspection Office.
Culture medium：Maconkey agar culture medium is commercial goods, is conventionally prepared, and sterilizing 4 DEG C of refrigerators of postposition are protected
Deposit spare.
Experimental animal：1 aa broiler chicken is purchased from Shaanxi Province's fowl industry baby chick supply base, exempts from without avian Escherichia coli bacteria vaccine
Epidemic disease, routinely feeding management are confirmed without any Escherichia coli symptom and other diseases in laboratory inner sealing breeding observing 14 days
It is used to test after symptom.Complete feed of the feeding without any antimicrobial, free water during experiment.
The preparation of bacterium solution：By Escherichia coli O78It is inoculated on maconkey agar tablet, when 37 DEG C of cultures 18 are small, after pure inspection,
Nutrient agar is inoculated in, when 37 DEG C of cultures 20 are small, is calculated by nephometer number method, is configured to bacterial concentration as 109The bacterium of cfu/ml
Liquid.
Experiment packet：Test chicken is randomly divided into 12 groups, every group 100, is specifically grouped through being tested after health examination
Situation refers to table 2：
Table 2
The equal chest muscle Escherichia Coli Injection bacterium solution of remaining each group chicken in addition to healthy control group, there is large intestine bar in test chicken after attacking poison
Bacterium disease classical symptom, its classical symptom are spiritual depressed, anorexia, and loose random, eye closing necking down, has difficulty in breathing, diarrhea, sternly
Severe one occurs dead.The visible liver hyperemia swelling of dissect, there is fibrinous exudate on surface；Constrictive pericarditis, hydropericardium, outer membrane
There is fibrinous exudate；Air bag thickens muddiness, there is cheesy thing exudate.By doing morphology, biochemistry to separated bacterium
Reaction and serological identification, are determined as chicken colibacillosis.
After chicken attacks poison morbidity, each group chicken is treated by designing scheme immediately.Chinese medicine test group and drug control group are equal
By 1.0g/L, drink is mixed, 3 days as a treatment course are used in conjunction.
Curative effect determinate standard mitigates rapidly for chicken clinical symptoms, recovers fast, and weight gain is obvious.Otherwise it is invalid to be judged to.Together
When, record the death rate, effective percentage, cure rate and the rate of body weight gain situation of each group.Data progress significance test, the wherein death rate,
Efficient and cure rate X2Examine；Weightening is examined using t.
Result of the test：Test chicken is after medicine mixes drink administration, and compared with infecting control group, clinical symptoms mitigate rapidly, extensive
Multiple fast, weight gain is obvious.During experiment, the death rate of each group chicken, efficient, cure rate and rate of body weight gain refer to table 3.
Table 3
| Group | Death rate % | Efficient % | Cure rate % | Body weight increase rate % |
| Healthy control group | 0 | —— | —— | —— |
| Infect control group | 83.00 | 17.00 | 17.00 | 70.24 |
| Group 1 | 20.00 | 80.00 | 77.00 | 88.07 |
| Group 2 | 11.00 | 89.00 | 86.00 | 93.25 |
| Group 3 | 5.00 | 95.00 | 93.00 | 96.26 |
| Group 4 | 15.00 | 85.00 | 83.00 | 92.01 |
| Group 5 | 10.00 | 90.00 | 89.00 | 94.05 |
| Group 6 | 9.00 | 90.00 | 88.00 | 95.08 |
| Group 7 | 12.00 | 88.00 | 85.00 | 92.85 |
| Group 8 | 18.00 | 82.00 | 80.00 | 90.01 |
| Ofloxacin soluble powder | 7.00 | 93.00 | 92.00 | 96.03 |
| Florfenicol powder | 9.00 | 91.00 | 90.00 | 95.03 |
X2Inspection shows eight group groups, Ofloxacin soluble powder group, florfenicol powder group compared with infecting control group, extremely
The rate of dying significantly reduces (P ＜ 0.01), wherein organize 3 groups, group 6 groups with Ofloxacin soluble powder group, florfenicol powder group difference not
Significantly (P ＞ 0.05)；T, which is examined, to be shown：Eight Chinese medicine test groups, Ofloxacin soluble powder group, florfenicol powder group and infection
Control group is compared, and average growth rate is significantly higher than infection control group (P ＜ 0.01), and organizes 3, group 6 and Ofloxacin soluble powder
Group, florfenicol powder group difference is not notable (P ＞ 0.05).By above-mentioned data analysis, learn that the therapeutic effect in group 3 is best,
For the Clinical practice effect of validation group 3, the ad hoc following clinical tests of meter, wherein, the Chinese medicine composition of group 3 in embodiment 1
Adding proportion is：Medicine is made into liquid according to 1.0g/L, whole day amount of drinking water is calculated according to chicken age in days, then further according to whole day
Amount of drinking water pours out the amount of medicine used, concentrates disposably drunk for diseased chicken after mixing.
Clinical test 2
Address：Tianjin Jinghai County Xiao Wang village it is honest and just certain
Kind：AA Broiler chicks
Age in days：37 ages in days
Incidence：Spiritual depressed, the loose random of chicken group, draws yellowish-white, yellow green loose stools, and feed intake declines, the death rate
7%, dissect visible slight fibrinous pericarditis and perihepatitis, intestinal mucosa is thickened or come off, and enteron aisle diffusivity bleeding.
Take pathological material of disease to be diagnosed through laboratory, be judged to Escherichia coli, the Chinese medicine composition of group 3 in embodiment 1 is proportionally added into drinking-water,
It is used in conjunction 3 days, the death rate reduces, and feed intake substantially rises, and excrement recovers normal, obtains good result.
Clinical test 3
Address：Shandong Province Pingdu Zhang
Kind：817 Broiler chicks
Age in days：12 ages in days
Incidence：Spiritual depressed, the loose random of chicken group, there is individually respiratory symptom, and feed intake declines, the death rate
23%, yellow-white stench excrement is drawn, the visible trachea-bronchial epithelial cell of dissect has slight bleeding, and air bag is muddy, pericarditis, perihepatitis, intestines
Mucous membrane comes off, and foul smelling taste.Take pathological material of disease to be diagnosed through laboratory, be judged to avian colibacillosis, by group 3 in embodiment 1
Drug composition is added in drinking-water in proportion, is freely drunk for fowl, is used in conjunction 3 days by this dosage, and the death rate reduces, and feed intake is obvious
Rise, excrement recovers normal, obtains good result.
Clinical test 4
Address：Liaoning Shenyang Lee
Kind：AA Broiler chicks
Age in days：17 ages in days
Incidence：Spiritual depressed, the loose random of chicken group, there is individually respiratory symptom, and feed intake declines, the death rate
22%, yellow-white stench excrement is drawn, the visible trachea-bronchial epithelial cell of dissect has slight bleeding, and air bag is muddy, pericarditis, perihepatitis, intestines
Mucous membrane comes off, and foul smelling taste.Take pathological material of disease to be diagnosed through laboratory, be judged to avian colibacillosis, by group 3 in embodiment 1
Drug composition is added in drinking-water in proportion, is freely drunk for fowl, is used in conjunction 3 days by this dosage, and the death rate reduces, and feed intake is obvious
Rise, excrement recovers normal, obtains good result.
Clinical test 5
Address：Nanjing Mr. Wang
Kind：Section is precious
Age in days：20 ages in days
Incidence：Jumpbogroup has 10% spiritual depressed, loose random, draws yellow loose stools, water sample loose stools, and feed intake declines,
Perianal fecal pollution, the death rate 3%, dissect duodenum have catarrhal inflammation, and jejunal hemorrhage, mucous membrane swelling, take off
Fall, cecal tonsil swelling bleeding, through bacteria distribution culture, be determined as colibacillosis, special addition is implemented in proportion in water
The Chinese medicine composition of group 3, is used in conjunction 3 days in example 1, and the death rate substantially reduces, and feed intake rises, and excrement substantially recovers, chooses live chickens and cut open
It is normal to examine the recoveries such as intestinal mucosa, treatment colibacillosis obtains good result.
Clinical test 6
Address：Shaanxi Dali Jiang
Kind：AA Broiler chicks
Age in days：18 ages in days
Incidence：Chicken group's mortality increases, and canthus is elongated in jumpbogroup, and the chicken increase dozed off of closing one's eyes, feed intake is reduced,
The visible claw of the chicken that dies of illness is dry, and anus has yellowish-white loose stool to pollute；Analyse it is visible there is pericardium yellow hydrops, have yellow-white on liver
Fibrinous exudate, the slight swelling of kidney, severe muscle aubergine, fibrinous pericarditis, perihepatitis, peritonitis and gas
Capsulitis, while the enteron aisle congestion of blood vessel is serious, intestinal fat bleeding, the symptom such as kidney swelling, is diagnosed as colibacillosis, then makes
With the Chinese medicine composition of group 3 in embodiment 1, jumpbogroup spirit is clearly better after medication three days, closes one's eyes, chicken of dozing off significantly reduces, and adopts
Appetite increase, excrement substantially improve, without the chicken that dies of illness.
Above-mentioned reference embodiment carries out a kind of Chinese medicine composition of chicken colibacillosis resisting disease and preparation method thereof detailed
Thin description, is illustrative rather than limited, several embodiments can be included according to limited scope, therefore do not taking off
From changing and modifications under present general inventive concept, should belong within protection scope of the present invention.
Claims (7)
- A kind of 1. Chinese medicine composition of chicken colibacillosis resisting disease, it is characterised in that：It is made of rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis, 10 parts of rheum officinale based on its parts by weight, 20 parts of rhizoma cyperi, 10 parts of radix astragali, 20 parts of Herba Andrographitis.
- 2. the preparation method of the Chinese medicine composition of the chicken colibacillosis resisting disease described in claim 1, it is characterised in that：By prescription ratio Example is by the Chinese medicine is extracted, medicinal extract is made in refined, concentration technology.
- 3. the preparation method of the Chinese medicine composition of chicken colibacillosis resisting disease according to claim 2, it is characterised in that：Specifically Preparation process is as follows：(1) it is raw material to weigh rheum officinale, rhizoma cyperi, radix astragali and Herba Andrographitis in prescription ratio, is uniformly mixed；(2) medicinal material 4-10 times of water measured of gross weight is added into above-mentioned four tastes Chinese medicine, is decocted 2 times, it is small to decoct 1.5-2.0 for the first time When, when second of decoction 1.0-1.5 is small, collecting decoction, filtration, when filtrate standing 12-36 is small, takes supernatant to be concentrated, adds Enter ethanol to alcohol content 60-70% (v/v), place, filtration；(3) relative density 1.35-1.4 is concentrated the filtrate to, and recycles ethanol, that is, obtains medicinal extract.
- 4. the preparation method of the Chinese medicine composition of chicken colibacillosis resisting disease according to claim 3, it is characterised in that：To leaching Excipient is added in cream particle is made.
- 5. the preparation method of the Chinese medicine composition of chicken colibacillosis resisting disease according to claim 4, it is characterised in that：It is described Excipient is Icing Sugar and/or dextrin.
- 6. the preparation method of the Chinese medicine composition of chicken colibacillosis resisting disease according to claim 5, it is characterised in that：To leaching Icing Sugar is added in cream particle is made with dextrin, wherein, the mass ratio of medicinal extract and Icing Sugar, dextrin is 1:2-3:2-3.
- 7. the preparation method of the Chinese medicine composition of chicken colibacillosis resisting disease according to claim 4, it is characterised in that：It is described Particle is dried under the conditions of 60-70 DEG C, crosses 10-30 mesh sieves twice in succession.
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