CN104304686B - DDGS fermented feed additive, fermented feed and preparation method of fermented feed - Google Patents
DDGS fermented feed additive, fermented feed and preparation method of fermented feed Download PDFInfo
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- CN104304686B CN104304686B CN201410657977.0A CN201410657977A CN104304686B CN 104304686 B CN104304686 B CN 104304686B CN 201410657977 A CN201410657977 A CN 201410657977A CN 104304686 B CN104304686 B CN 104304686B
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Abstract
The invention relates to the field of biotechnology, in particular to a DDGS fermented feed additive, fermented feed and a preparation method of the fermented feed. The DDGS fermented feed additive comprises a composite microbial strain, a medium temperature amylase and a strong base. The composite microbial strain comprises lactobacilli, bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae. DDGS is fermented by means of microorganisms, and crude fiber, non-starch polysaccharides and the like in the DDGS can be degraded through the metabolism of the microorganisms, so that the protein content is increased, and protein macromolecules are degraded into small peptides and other small easily-absorbed molecules; meanwhile, due to a large number of beneficial microorganisms propagated in the fermentation process, a large number of lactic acids and other organic acids generated in the fermentation process, and the environment with the lower pH value, molds in the DDGS can be inhibited; due to the lactic acids and the other organic acids generated in the fermentation process, the feed can have the fragrant and sweet taste, and the DDGS amount added in the feed is further increased.
Description
Technical field
The present invention relates to biological technical field, particularly to a kind of additive of DDGS fermented feed, fermented feed and its
Preparation method.
Background technology
DDGS (Distillers Dried Grains with Solubles, Semen Maydiss distiller's dried grain and soluble substance) is with jade
Rice for being processed the maize alcohol lees obtaining and residual liquid afterwards to poor liquid during main raw material(s) preparing ethanol by fermentation
Dried object, its do not contain only rich in protein, aminoacid, vitamin, fat, fermented distilled during generate unknown growth
The factor, is also rich in the several mineral materials being conducive to growth of animal.Therefore, DDGS, can be extensively used as a kind of protein feedss raw material
It is applied to Production of Livestock and Poultry.But the potential mycotoxin contamination of DDGS easily produces impact to animal health and production performance, and
In DDGS, the fiber of high-load and non-starch polysaccharides(nsp) can affect the absorption of the mineral ions such as phosphorus, reduce the utilization of nutrient substance
Rate, and its poor palatability etc. limits utilization in feedstuff for the DDGS.
Solid microbe fermentation technology is applied relatively broad in feedstuff industry, and its main purpose is by solid fermentation
Mode, to improve the quality of product, improves palatability, reduces the antinutritional factor content in raw material, improves digesting and assimilating of feedstuff
Rate is so as to become the feed product of natural nuisance-free high nutrition.But current DDGS feedstuff does not have related microorganisms solid fermentation
Report.Therefore it provides a kind of additive of DDGS fermented feed, fermented feed and preparation method thereof have realistic meaning.
Content of the invention
In view of this, the present invention provides a kind of additive of DDGS fermented feed, fermented feed and preparation method thereof.Using
Microorganism carries out fermentation process to DDGS, by the metabolism of microorganism can degrade crude fibre in DDGS and non-starch many
Sugar etc., improves protein content, and protein macromolecule is degraded to the small-molecule substance that small peptide etc. is easy to absorb；Ferment simultaneously
Process can prolific beneficial microbe, and the organic acid such as substantial amounts of lactic acid producing and relatively low pH environment can play
Inhibitory action to the mycete in DDGS, the organic acid such as lactic acid that fermentation produces makes feedstuff have sour fragrant and sweet taste, can improve very well
DDGS palatability, can improve addition in feedstuff for the DDGS further.
In order to realize foregoing invention purpose, the present invention provides technical scheme below：
The invention provides a kind of additive of DDGS fermented feed, it includes composite bacteria, midrange thermal stable amylase and highly basic；
Described composite bacteria includes lactobacilluss, bacillus subtilises, Bacillus licheniformis, saccharomyces cerevisiae.
In some embodiments of the invention, the mass ratio of described composite bacteria, described midrange thermal stable amylase and described highly basic
For 3～5:4～6:1～3.
In some embodiments of the invention, the mass ratio of described composite bacteria, described midrange thermal stable amylase and described highly basic
For 3～5:4～6:1.
In some embodiments of the invention, lactobacilluss described in described composite bacteria, described bacillus subtilises, institute
State Bacillus licheniformis, the mass ratio of described saccharomyces cerevisiae is 1～5:1～3:1～3:1～3.
Preferably, lactobacilluss described in described composite bacteria, described bacillus subtilises, described lichens spore bar
Bacterium, the mass ratio of described saccharomyces cerevisiae are 3:2:2:2.
Present invention also offers a kind of DDGS fermented feed, by fermentation raw material DDGS and comprise above-mentioned additive and water by fermentation
Make.
In some embodiments of the invention, the mass ratio of described fermentation raw material DDGS, described additive and water be 28～
47:8～12:45～60.
Preferably, the mass ratio of described fermentation raw material DDGS, described additive and water is 28～47:8～12:55.I.e.
Preferably, in DDGS fermented feed, the weight/mass percentage composition of moisture is 55%.
In some embodiments of the invention, described ferment for solid fermentation.
In some embodiments of the invention, described fermentation temperature is 35～40 DEG C, and described fermentation time is 3～5d.
Preferably, described fermentation temperature is 37 DEG C, described fermentation time is 4d
In some embodiments of the invention, the step being additionally included in 50～80 DEG C of dryings after described fermentation.As excellent
Choosing, the temperature being dried after fermentation is 65 DEG C.
Present invention also offers the preparation method of above-mentioned DDGS fermented feed it is characterised in that take fermentation raw material DDGS and
Comprise as fermented prepared in above-mentioned additive and water；The temperature of described fermentation is 35～40 DEG C, time of described fermentation is 3～
5d.The step being additionally included in 50～80 DEG C of dryings after described fermentation.Preferably, the temperature being dried after fermentation is 65 DEG C.
The present invention provides a kind of additive of DDGS fermented feed, fermented feed and preparation method thereof.This additive, its bag
Include composite bacteria, midrange thermal stable amylase and highly basic；Described composite bacteria includes lactobacilluss, bacillus subtilises, lichens spore bar
Bacterium, saccharomyces cerevisiae.Using microorganism, fermentation process is carried out to DDGS, can be degraded in DDGS by the metabolism of microorganism
Crude fibre and non-starch polysaccharides(nsp) etc., improve protein content, and by protein macromolecule be degraded to small peptide etc. be easy to absorb little
Molecular substance；Simultaneously sweat can prolific beneficial microbe, and the organic acid such as substantial amounts of lactic acid that produces and relatively
Low pH environment can play the inhibitory action to the mycete in DDGS, and the organic acid such as lactic acid that fermentation produces makes feedstuff have acid
Fragrant and sweet taste, can improve DDGS palatability very well, can improve addition in feedstuff for the DDGS further.
The product being obtained by the inventive method, after inspection, the key technical indexes is：
(1) product gross protein value >=30%；
(2) the little peptide content of product (accounting for thick protein) >=18%；
(3) product lactic acid content (in terms of total acid) >=3%.
Specific embodiment
The invention discloses a kind of additive of DDGS fermented feed, fermented feed and preparation method thereof, art technology
Personnel can use for reference present disclosure, be suitably modified technological parameter and realize.Specifically, all similar replacements and changing
Dynamic apparent to those skilled in the art, they are considered as including in the present invention.The method of the present invention and should
With being described by preferred embodiment, related personnel substantially can be without departing from present invention, spirit and scope
Method described herein and application are modified or suitably change and combine, to realize and to apply the technology of the present invention.
Raw materials used and reagent in the additive of DDGS fermented feed of present invention offer, fermented feed and preparation method thereof
All can be buied by market.Composite bacterium powder used by the present invention is sold company limited by Dingzhou posivtive spike feedstuff and is buied.
With reference to embodiment, the present invention is expanded on further：
Embodiment 1
1 materials and methods
1.1 strains, amylase and source
Composite bacterium powder used by test is made up of lactobacilluss, bacillus subtilises, Bacillus licheniformis, saccharomyces cerevisiae, on
The ratio stating four kinds of bacterium is 3:2:2:2, midrange thermal stable amylase used is fermented and rear extractive technique is refined by bacillus subtilises
The diastase being refined.
1.2DDGS raw material sources
DDGS raw material is the Semen Maydiss dry alcohol groove obtaining after poor liquid being processed during preparing ethanol by fermentation.
1.3 EXPERIMENTAL DESIGN
Weigh DDGS 200g, add moisture in the 45% of fermentation material total amount, 50%, 55%, 60% ratio, be designated as respectively
M1、M2、M3、M4.NaOH, midrange thermal stable amylase and composite bacteria weigh by the 1% of DDGS dry matter content, 5 ‰, 4 ‰ respectively and add
Plus, it is mixed into after noting during mixing first dissolving NaOH in DDGS raw material, adds midrange thermal stable amylase and composite bacteria afterwards, mix, often
Individual 3 repetitions of process.Fermentation temperature controls at 37 DEG C, fermentation time 4d, 65 DEG C of drying after fermentation ends, crosses 40 mesh after crushed
Sieve, and measure the indexs such as thick protein, small peptide, lactic acid.
1.4 chemical analyses
Gross protein value detects：Specify execution by GB/T 5009.5-2003.
Small peptide content detection adopts trichloroacetic acid extraction method：Accurately weigh sample 1.5g (being accurate to 0.0001g) in 100mL
In beaker, add 15% solution of trichloroacetic acid 25mL, vibrate 5min, stand 5min, solution is quantitatively shifted, at 3,000 rpm
Centrifugation 10min, takes whole supernatant liquid filterings, accurately pipettes 10mL filtrate and be placed in digestive tube, by GB/T 5009.5-2003 side
Method digests and measures protein content.Small peptide computing formula is as follows：
Small peptide (accounting for thick protein, %)=(V-V0)*C*6.25*0.014*2.5*100/(M*CP)
In formula：The volume of V sample consumption HCl, mL；
V0The blank volume consuming HCl, mL；
The molar concentration of C hydrochloric acid, mol/L；
M sample quality, g；
6.25*0.014 albumen conversion coefficient；
The gross protein value of CP sample.
Lactic acid content (in terms of total acid) adopts NaOH titration measuring：Weigh 0.5g sample (being accurate to 0.0002g) in
In 250mL conical flask, add 100mL distilled water, 60 DEG C of water-bath 30min, take 40mL supernatant, add 2～3 phenolphthalein instructions
Agent, is titrated to pink with standard NaOH solution, and 30s is colour-fast.
Lactic acid (in terms of total acid, %)=(V-V0)*C*0.09008*100/(M*40/100)
In formula：The volume of V sample consumption NaOH, mL；
V0The blank volume consuming NaOH, mL；
0.09008 every milliequivalent NaOH is equivalent to the grams of lactic acid；
M sample quality, g.
PH measures：Weigh 5g sample to be placed in 100mL triangular flask, add 45mL distilled water, after shaking up, stand 30min, use
Determination of electrode pH.
1.5 data processing
Data carries out one factor analysis of variance using SPSS16.0 software, carries out multiple comparisons using Duncan method.Measure
Result is represented with " mean+SD ".
2 results and analysis
The impact that the chemical composition of table 1 DDGS raw material and moisture addition ferment to DDGS
Note：Same column shoulder mark is different to represent significant difference (p<0.05)
As shown in Table 1, after DDGS fermentation, thick protein and little peptide content are significantly increased (p<0.05), though lactic acid
Reduce, but caused by being practically due to add NaOH in DDGS fermentation raw material.DDGS acidity itself is higher, and pH value is relatively low, is
Ensure composite bacteria normal growth and ferment effect, therefore add appropriate NaOH in DDGS raw material.Raise in the fermentation of each group DDGS
In material, M3The thick protein of group (moisture addition is 55%) and lactic acid content are respectively 30.94%, 3.39%, are significantly higher than
M1、M2、M4Three groups；PH is 4.70, less than other three groups；And little peptide content is 19.93% (accounting for thick protein), with other three groups
It is not significantly different from.Comprehensive each Indexes Comparison, finds M3Group ferment effect be better than other three groups, that is, add 55% moisture to send out
Ferment effect is best.
Embodiment 2
1 materials and methods
1.1 strain, amylase and source
Composite bacterium powder used by test is made up of lactobacilluss, bacillus subtilises, Bacillus licheniformis, saccharomyces cerevisiae, on
The ratio stating four kinds of bacterium is 3:2:2:2, midrange thermal stable amylase used is fermented and rear extractive technique is refined by bacillus subtilises
The diastase being refined.
1.2DDGS raw material sources
DDGS raw material is the Semen Maydiss dry alcohol groove obtaining after poor liquid being processed during preparing ethanol by fermentation.
1.3 EXPERIMENTAL DESIGN
Weigh DDGS 200g, add moisture in 55% ratio of fermentation material total amount.NaOH, midrange thermal stable amylase and compound bacteria
Plant 1%, 4 ‰, the 3 ‰ weighing interpolations pressing DDGS dry matter content respectively, after noting during mixing first dissolving NaOH, be mixed into DDGS
In raw material, add midrange thermal stable amylase and composite bacteria afterwards, mix, each processes 3 repetitions.Fermentation temperature controls at 37 DEG C, sends out
Ferment time 4d, 65 DEG C of drying after fermentation ends, cross 40 mesh sieves after crushed, and measure the indexs such as thick protein, small peptide, lactic acid.
1.4 chemical analyses
Gross protein value detects：Specify execution by GB/T 5009.5-2003.
Small peptide content detection adopts trichloroacetic acid extraction method：Accurately weigh sample 1.5g (being accurate to 0.0001g) in 100mL
In beaker, add 15% solution of trichloroacetic acid 25mL, vibrate 5min, stand 5min, solution is quantitatively shifted, at 3,000 rpm
Centrifugation 10min, takes whole supernatant liquid filterings, accurately pipettes 10mL filtrate and be placed in digestive tube, by GB/T 5009.5-2003 side
Method digests and measures protein content.Small peptide computing formula is as follows：
Small peptide (accounting for thick protein, %)=(V-V0)*C*6.25*0.014*2.5*100/(M*CP)
In formula：The volume of V sample consumption HCl, mL；
V0The blank volume consuming HCl, mL；
The molar concentration of C hydrochloric acid, mol/L；
M sample quality, g；
6.25*0.014 albumen conversion coefficient；
The gross protein value of CP sample.
Lactic acid content (in terms of total acid) adopts NaOH titration measuring：Weigh 0.5g sample (being accurate to 0.0002g) in
In 250mL conical flask, add 100mL distilled water, 60 DEG C of water-bath 30min, take 40mL supernatant, add 2～3 phenolphthalein instructions
Agent, is titrated to pink with standard NaOH solution, and 30s is colour-fast.
Lactic acid (in terms of total acid, %)=(V-V0)*C*0.09008*100/(M*40/100)
In formula：The volume of V sample consumption NaOH, mL；
V0The blank volume consuming NaOH, mL；
0.09008 every milliequivalent NaOH is equivalent to the grams of lactic acid；
M sample quality, g.
PH measures：Weigh 5g sample to be placed in 100mL triangular flask, add 45mL distilled water, after shaking up, stand 30min, use
Determination of electrode pH.
1.5 data processing
Data carries out one factor analysis of variance using SPSS16.0 software, carries out multiple comparisons using Duncan method.Measure
Result is represented with " mean+SD ".
2 results and analysis
Chemical composition before and after table 2 DDGS fermenting raw materials
Note：Same column shoulder mark is different to represent significant difference (p<0.05)
As shown in Table 2, after DDGS fermentation, thick protein and little peptide content are significantly increased (p<0.05), respectively reach
30.52%, 18.86% (accounting for thick protein), with the addition of NaOH because in fermentation raw material, therefore lactic acid content is lower than raw material, but still
Reach 3.02 (in terms of total acids).
Embodiment 3
1 materials and methods
1.1 strains, amylase and source
Composite bacterium powder used by test is made up of lactobacilluss, bacillus subtilises, Bacillus licheniformis, saccharomyces cerevisiae, on
The ratio stating four kinds of bacterium is 3:2:2:2, midrange thermal stable amylase used is fermented and rear extractive technique is refined by bacillus subtilises
The diastase being refined.
1.2DDGS raw material sources
DDGS raw material is the Semen Maydiss dry alcohol groove obtaining after poor liquid being processed during preparing ethanol by fermentation.
1.3 EXPERIMENTAL DESIGN
Weigh DDGS 200g, add moisture in 55% ratio of fermentation material total amount.NaOH, midrange thermal stable amylase and compound bacteria
Plant 1%, 6 ‰, the 5 ‰ weighing interpolations pressing DDGS dry matter content respectively, after noting during mixing first dissolving NaOH, be mixed into DDGS
In raw material, add midrange thermal stable amylase and composite bacteria afterwards, mix, each processes 3 repetitions.Fermentation temperature controls at 37 DEG C, sends out
Ferment time 4d, 80 DEG C of drying after fermentation ends, cross 40 mesh sieves after crushed, and measure the indexs such as thick protein, small peptide, lactic acid.
1.4 chemical analyses
Gross protein value detects：Specify execution by GB/T 5009.5-2003.
Small peptide content detection adopts trichloroacetic acid extraction method：Accurately weigh sample 1.5g (being accurate to 0.0001g) in 100mL
In beaker, add 15% solution of trichloroacetic acid 25mL, vibrate 5min, stand 5min, solution is quantitatively shifted, at 3,000 rpm
Centrifugation 10min, takes whole supernatant liquid filterings, accurately pipettes 10mL filtrate and be placed in digestive tube, by GB/T 5009.5-2003 side
Method digests and measures protein content.Small peptide computing formula is as follows：
Small peptide (accounting for thick protein, %)=(V-V0)*C*6.25*0.014*2.5*100/(M*CP)
In formula：The volume of V sample consumption HCl, mL；
V0The blank volume consuming HCl, mL；
The molar concentration of C hydrochloric acid, mol/L；
M sample quality, g；
6.25*0.014 albumen conversion coefficient；
The gross protein value of CP sample.
Lactic acid content (in terms of total acid) adopts NaOH titration measuring：Weigh 0.5g sample (being accurate to 0.0002g) in
In 250mL conical flask, add 100mL distilled water, 60 DEG C of water-bath 30min, take 40mL supernatant, add 2～3 phenolphthalein instructions
Agent, is titrated to pink with standard NaOH solution, and 30s is colour-fast.
Lactic acid (in terms of total acid, %)=(V-V0)*C*0.09008*100/(M*40/100)
In formula：The volume of V sample consumption NaOH, mL；
V0The blank volume consuming NaOH, mL；
0.09008 every milliequivalent NaOH is equivalent to the grams of lactic acid；
M sample quality, g.
PH measures：Weigh 5g sample to be placed in 100mL triangular flask, add 45mL distilled water, after shaking up, stand 30min, use
Determination of electrode pH.
1.5 data processing
Data carries out one factor analysis of variance using SPSS16.0 software, carries out multiple comparisons using Duncan method.Measure
Result is represented with " mean+SD ".
2 results and analysis
Chemical composition before and after table 3 DDGS fermenting raw materials
Note：Same column shoulder mark is different to represent significant difference (p<0.05)
As shown in Table 3, after DDGS fermentation, thick protein and little peptide content are significantly increased, and respectively reach 31.40%,
19.13% (accounting for thick protein), with the addition of NaOH because in fermentation raw material, therefore lactic acid content is lower than raw material, but still reach
3.27% (in terms of total acid).
Embodiment 4 fermentation checking test on a small scale
1. strain, amylase and source
Composite bacterium powder used by test is made up of lactobacilluss, bacillus subtilises, Bacillus licheniformis, saccharomyces cerevisiae, on
The ratio stating four kinds of bacterium is 3:2:2:2, midrange thermal stable amylase used is fermented and rear extractive technique is refined by bacillus subtilises
The diastase being refined.
2.DDGS raw material sources
DDGS raw material is the Semen Maydiss dry alcohol groove obtaining after poor liquid being processed during preparing ethanol by fermentation.
3. fermentation material prepares
Weigh 5kg DDGS raw material, and in 1%, 5 ‰, 4 ‰ ratio weigh respectively 50g NaOH, 25g midrange thermal stable amylase,
20g composite bacteria is stand-by.Measure the warm water (about 37 DEG C) of fermentation material total amount 55%, a part adds DDGS after being used for dissolving NaOH
In raw material, mix thoroughly, a part adds in DDGS raw material after being used for dissolving midrange thermal stable amylase and composite bacteria, mix homogeneously.
4. ferment
After inoculating and in the DDGS loading Nylon Bag of mix homogeneously, and seal intact, be placed in fermentation in 37 DEG C of incubators
Take out after 4d.
5. dry
Fermentation DDGS is dried through 80 DEG C, obtains final product fermented product.
6. detect
After testing the gross protein value of fermented sample be 31.14%, small peptide be 19.97% (accounting for thick protein), lactic acid
Content is 3.36%, such as table 4.
Chemical composition before and after the small-scale fermentation of table 4 DDGS raw material
As can be seen here, the additive that the present invention provides and DDGS fermented feed can improve the utilization rate of nutrient substance.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of additive for DDGS fermentation is it is characterised in that be made up of composite bacteria, midrange thermal stable amylase and highly basic；
Described composite bacteria is made up of lactobacilluss, bacillus subtilises, Bacillus licheniformis, saccharomyces cerevisiae.
2. additive according to claim 1 is it is characterised in that described composite bacteria, described midrange thermal stable amylase and described
The mass ratio of highly basic is 3～5:4～6:1～3.
3. additive according to claim 1 is it is characterised in that described composite bacteria, described midrange thermal stable amylase and described
The mass ratio of highly basic is 3～5:4～6:1.
4. additive according to claim 1 is it is characterised in that lactobacilluss described in described composite bacteria, described withered
Careless bacillus cereuss, described Bacillus licheniformis, the mass ratio of described saccharomyces cerevisiae are 1～5:1～3:1～3:1～3.
5. additive according to claim 1 is it is characterised in that lactobacilluss described in described composite bacteria, described withered
Careless bacillus cereuss, described Bacillus licheniformis, the mass ratio of described saccharomyces cerevisiae are 3:2:2:2.
6. a kind of DDGS fermented feed is it is characterised in that by fermentation raw material DDGS and comprise as any one of claim 1 to 5 institute
State additive and water by fermentation is made.
7. DDGS fermented feed according to claim 6 is it is characterised in that described fermentation raw material DDGS, described additive
Mass ratio with water is 28～47:8～12:45～60.
8. DDGS fermented feed according to claim 6 is it is characterised in that described fermentation is solid fermentation.
9. DDGS fermented feed according to claim 6 is it is characterised in that described fermentation temperature is 35～40 DEG C, described
Fermentation time is 3～5d.
10. the preparation method of the DDGS fermented feed according to any one of claim 6 to 9 is it is characterised in that take proferment
Material DDGS and comprise that additive and water as described in any one of claim 1 to 5 are fermented to be obtained；The temperature of described fermentation be 35～
40 DEG C, the time of described fermentation is 3～5d.
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| CN111631316A (en) * | 2020-06-11 | 2020-09-08 | 河南白云牧港生物科技有限公司 | Traditional Chinese medicine fermented feed containing enzyme bacteriostat and preparation method thereof |
| CN112998123A (en) * | 2021-04-27 | 2021-06-22 | 吉林农业大学 | Fermentation process for improving alcohol soluble protein content of DDGS feed |
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| CN101919490A (en) * | 2009-06-12 | 2010-12-22 | 胡徐玉 | Method for producing biological feed by biological fermentation of liquor distiller grains |
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