CN104288278B - Use of Periplaneta americana and Radix Sanguisorbae in combination drug - Google Patents
Use of Periplaneta americana and Radix Sanguisorbae in combination drug Download PDFInfo
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- CN104288278B CN104288278B CN201410326015.7A CN201410326015A CN104288278B CN 104288278 B CN104288278 B CN 104288278B CN 201410326015 A CN201410326015 A CN 201410326015A CN 104288278 B CN104288278 B CN 104288278B
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/739—Sanguisorba (burnet)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7007—Drug-containing films, membranes or sheets
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7023—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
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Abstract
The invention provides a use of Periplaneta americana or its extract and Radix Sanguisorbae or its extract in the preparation of an external bleeding stopping and wound healing promoting combination drug. The combination drug comprises, by weight, 1-10 parts of Periplaneta americana or its extract and 1-10 parts of Radix Sanguisorbae or its extract. The combination use of Periplaneta americana and Radix Sanguisorbae in the invention significantly increases the wound restoration effect and the bleeding stopping effect of drugs, have same or even better effects than a classic drug Yunnan Baiyao, and provides a new choice for the clinic drug use.
Description
Technical field
The present invention relates to American cockroach and the drug combination new application of garden burnet.
Background technology
Skin is the maximum organ of human body, carry protection body, perspire, cold and pressure the function of sensible heat.Skin covers
Lid whole body, it make in vivo it is various tissue and organ from physical, mechanicalness, chemically with the invasion and attack of pathogenic microorganism.
Through being scratched, wound, burn and fester etc. and to cause skin damage after, it is easy to cause bacterium infection, body fluid is lost in and causes each
Plant complication.It is foundation according to wet union theory, modern treatment of wounds all takes moisture treatment, as wound to build a profit
In the microenvironment (micro- acid, anaerobic or hypoxemia, appropriateness moistening) of tissue growth.Prepare can effectively stop blooding, wound healing
Medicine be particularly important.
American cockroach Perip leneta ameri Cana, are the insects of volume maximum in Blattidae.Research finds, beautiful
There is the big Lian extracts in continent good wound repair to act on.Such as Kangfuxin Liquid, it is by American cockroach Perip leneta
The extract (i.e. rehabilitation is new) that ameri Cana dry polypide is prepared from, and in American-cockroach-extract, is calculated according to dry product,
7.0% (referring to WS3-B-3674-2000 (Z)) must not be less than containing total amino acid.Kangfuxin Liquid can be promoting blood circulation, nourishing yin and promoting granulation.
It is for oral administration：For stagnation of blood stasis, have a stomachache bleeding, taste-blindness rate；And deficiency of Yin tuberculosis, phthisical auxiliary treatment.Outward
With：For metal-inflicted wound, wound, ulcer, fistula, burn, scald, bedsore the surface of a wound.
Yet there are no the report that American cockroach and garden burnet are used in combination.
The content of the invention
It is an object of the invention to provide American cockroach and the drug combination new application of garden burnet.Present invention also offers one kind
Medical composition and its use.
Preparing external application hemostasis, promoting the surface of a wound with garden burnet or its extract the invention provides American cockroach or its extract
Purposes in the combination medicine of healing.
Wherein, the weight proportion of each bulk drug is in the combination medicine：1~10 part of American cockroach or its extract,
1~10 part of garden burnet or its extract.
Further, the weight proportion of each bulk drug is in the combination medicine：American cockroach or its extract 1~6
1~6 part of part, garden burnet or its extract.
Further, the weight proportion of each bulk drug is in the combination medicine：American cockroach or its extract 2~
1~6 part of 6 parts, garden burnet or its extract.
Preferably, the weight proportion of each bulk drug is in the combination medicine：4 parts of American cockroach or its extract,
2~6 parts of elm or its extract.
It is further preferred that the weight proportion of each bulk drug is in the combination medicine：American cockroach or its extract 4
Part, garden burnet or its extract 2 or 6 parts.
Wherein, the American-cockroach-extract dries carrying for polypide for American cockroach Perip leneta ameri Cana
Thing is taken, 7.0% is no less than containing total amino acid；The Radix Sangusorbae extract is garden burnet total tannin, and tannin contains in the garden burnet total tannin
Amount is more than 50%.
American-cockroach-extract of the present invention, meets in WS3-B-3674-2000 (Z) to " American cockroach Perip
Leneta ameri Cana dry the extract of polypide " limit standard, according to dry product calculate, containing total amino acid not
7.0% must be less than, such as commercially available rehabilitation is new.Alternatively, it is also possible to use American-cockroach-extract preparation method common at present, example
Such as：
American cockroach is taken, is crushed, 70~90%v/v ethanol is extracted, merge extract solution, after reclaiming ethanol, add water mixing, plus
Thermal agitation, stands, and cooling makes grease fully float, and removes clear liquid, concentrates or obtains final product American-cockroach-extract after drying.
Concentration of alcohol can select 80~90%v/v, such as 80%v/v, 81%v/v, 82%v/v, 83%v/v, 84%
V/v, 85%v/v, 86%v/v, 87%v/v, 88%v/v, 89%v/v, 90%v/v.
Garden burnet of the present invention is rosaceous plant garden burnet Sanguisorba officinalis L. or the garden burnet that comes into leaves
The dry root of Sanguisorba officinalis L.var.longifolia (Bert.) Yu et Li.
According to the relevant regulations of natural plants chemical field, total starches, general flavone, TA, total tannin etc. refer to carry
Take extract part respectively containing polysaccharide, flavones, alkaloid, tannin etc. more than 50% in thing.Ground used in the present invention
Elm total tannin, also corresponds to above-mentioned relevant regulations, and the wherein content of tannin can further select content 80% more than 50%
Garden burnet total tannin above, such as the garden burnet total tannin of content more than 85% of the present invention for illustrating.
The preparation of garden burnet total tannin, generally uses water, ethanol, hydrous ethanol, acetone or aqueous acetone for solvent is carried
Take, then the method such as combination through chromatography, precipitation of protein, solvent method or the above method carries out polishing purification.
Wherein, the chromatography can be adsorbed using gel, macroporous absorbent resin.
The solvent method, refer to will contain total tannin crude product aqueous degreaser treatment after, then with ethyl acetate extract, obtain final product
Garden burnet tannin extract；Or total tannin crude product is dissolved in ethanol and ethyl acetate, the Precipitation garden burnet tannin that adds diethyl ether is extracted
Thing.
The precipitation of protein, can be gelatin precipitation acetone analytic method more using the method precipitated with the gelatin.
Solvent method and gelatin precipitation acetone method can be used to combine the preparation for garden burnet total tannin in the present invention, its operation
Step is as follows：
(1) garden burnet medicinal material is taken, is crushed, add aqueous acetone to be extracted, collect extract solution, after reclaiming acetone, added water mixed
Close, staticly settle, take supernatant, be extracted with ethyl acetate, collect ethyl acetate extract layer, reclaim ethyl acetate, obtain final product tannin
Crude product；
(2) tannin crude product is taken, is dissolved in water, taken supernatant and add gelatin solution, staticly settled, after taking precipitation drying, used
Acetone or aqueous acetone dissolve, and collect supernatant, reclaim acetone, dry, and obtain final product garden burnet total tannin.
Wherein, the aqueous acetone concentration of step (1) be 60-80%v/v, preferably 65-75%v/v, such as 65%v/v,
66%v/v, 67%v/v, 68%v/v, 69%v/v, 70%v/v, 71%v/v, 72%v/v, 73%v/v, 74%v/v, 75%
V/v etc..
Aqueous acetone concentration is 80~95%v/v, preferably 85-95%v/v, such as 85%v/v, 86% in step (2)
V/v, 87%v/v, 88%v/v, 89%v/v, 90%v/v, 91%v/v, 92%v/v, 93%v/v, 94%v/v, 95%v/v
Deng.
Present invention also offers a kind of pharmaceutical composition, it is the system being prepared from by the bulk drug of following weight proportion
Agent：American cockroach or 1~10 part of 1~10 part of its extract, garden burnet or its extract.
Further, it is the preparation being prepared from by the bulk drug of following weight proportion：American cockroach or its extract 1
1~6 part of~6 parts, garden burnet or its extract.
Further, it is the preparation being prepared from by the bulk drug of following weight proportion：American cockroach or its extraction
1~6 part of 2~6 parts of thing, garden burnet or its extract.
Preferably, it is the preparation being prepared from by the bulk drug of following weight proportion：American cockroach or its extract 4
2~6 parts of part, garden burnet or its extract.
It is further preferred that it is the preparation being prepared from by the bulk drug of following weight proportion：American cockroach or its carry
Take 4 parts of thing, garden burnet or its extract 2 or 6 parts.
Wherein, the preparation is external preparation.
Further, the external preparation is patch, dressing, film, adhesive.
Further, the patch is hydrogel patch, foamed material patch；The adhesive is pressure sensitive adhesive.
Present invention also offers purposes of the aforementioned pharmaceutical compositions in hemostasis, the medicine of wound healing is prepared.
Further, the medicine is external used medicine.
After American cockroach and garden burnet are used in combination the present invention, repair and hemostasis of the medicine to the surface of a wound are significantly improved
Effect, and drug effect is suitable, even better with classical medicine Yunnan Baiyao, for clinical application provides new selection.
Specific embodiment
The hydrogel patch of embodiment 1
【Prescription】Raw material：American-cockroach-extract：0.4g garden burnet tannins 0.2g
Auxiliary material：Aluminum dihydroxyamino acetate 10g NP-70030g glycerine 30g borneol 20g PVP k30 10g
【Preparation method】In the 1000mL glass flasks with intense stirring device, by the medicine of recipe quantity and crosslinking agent dihydroxy
Base aluminum aminoacetate is dissolved in the mixed solution of NMF glycerine, penetrating agent and purified water, adds the water-setting matrix of recipe quantity
Material NP-700, is sufficiently stirred for, and is well mixed it；Again by pH adjusting agent tartaric acid, the increasing stick of well mixed recipe quantity
Agent PVP k30 and water for injection are added thereto, stirring, are well mixed it.Above-mentioned pastille hydrogel matrix material is used again
Doctor blade process, is coated on the non-woven fabrics by silicic acid anhydride, and it is (1.5 ± 0.1) mm to control coating thickness.It is multiple with anti-sticker
After conjunction, it is placed in and 1h is dried in 60 DEG C of baking ovens, to required degree, cutting, sealing is obtained final product regulation water content after cooling.
The liquid dressing of embodiment 2
【Prescription】Raw material：American-cockroach-extract：0.4g garden burnet tannins 0.2g
Auxiliary material：Chitin 20g borneol 20g collagens 30g
After said medicine is well mixed with dressing, distilled water constant volume is added to be mixed to 100ml.First by chitin,
Collagen and distilled water heating for dissolving, heating-up temperature are 80~100 DEG C, add the blending of inorganic acid, formaldehyde and foaming agent and stir
Mix；By blend solution hot briquetting, the temperature of thermoforming is 60~90 DEG C, and the time of thermoforming is 4~12 hours；Finally by heat
The product of shaping is cleaned, and is obtained final product.
The foamed material patch of embodiment 3
【Prescription】Raw material：American-cockroach-extract：0.4g garden burnet tannins 0.2g
Auxiliary material：Chitin 20g polyvinyl alcohol 25g
First by chitin, polyvinyl alcohol and distilled water heating for dissolving, heating-up temperature is 80~100 DEG C, and medicine is mixed into equal
After even in the auxiliary material of addition melting, blending and stirring；By blend solution hot briquetting, the temperature of thermoforming is 60~90 DEG C, heat into
The time of type is 4~12 hours；Finally the product of thermoforming is cleaned, is obtained final product.
The film of embodiment 4
【Prescription】Raw material：American-cockroach-extract：0.4g garden burnet tannins 0.2g
Auxiliary material：Polyvinyl alcohol 30g borneol 20g glycerine 10g CMC-Na 15g
First by polyvinyl alcohol heating for dissolving in glycerine, heating-up temperature is 80~100 DEG C, and borneol is finely ground rear thin with medicine
Powder and CMC-Na are sufficiently mixed into after medicine slurry in the auxiliary material for adding melting, blending and stirring.Then with film applicator film into required
Thickness, the area of UD film is calculated after drying according to drug content, cut into the small lattice of single dose, packaging is obtained final product.
The pressure sensitive adhesive of embodiment 5
【Prescription】Raw material：American-cockroach-extract：0.4g garden burnet tannins 0.2g
Auxiliary material：SIS thermoplastic elastomer (TPE) 15g C5 resin 10g mineral oil 5g acrylic resin 5g polyethylene glycol
10g diphenylamines 3g
First pass through banburying mode carries out physical blending by SIS types thermoplastic elastomer (TPE), acrylic resin etc. in advance, in melting
Add medicine and other constituents well mixed prepared under state.
The hydrogel patch of embodiment 6
【Prescription】Raw material：Rehabilitation is new：0.4g garden burnet tannins 0.2g
Auxiliary material：Aluminum dihydroxyamino acetate 10g NP-70030g glycerine 30g borneol 20g PVP k30 10g
【Preparation method】In the 1000mL glass flasks with intense stirring device, by the medicine of recipe quantity and crosslinking agent dihydroxy
Base aluminum aminoacetate is dissolved in the mixed solution of NMF glycerine, penetrating agent and purified water, adds the water-setting matrix of recipe quantity
Material NP-700, is sufficiently stirred for, and is well mixed it；Again by pH adjusting agent tartaric acid, the increasing stick of well mixed recipe quantity
Agent PVP k30 and water for injection are added thereto, stirring, are well mixed it.Above-mentioned pastille hydrogel matrix material is used again
Doctor blade process, is coated on the non-woven fabrics by silicic acid anhydride, and it is (1.5 ± 0.1) mm to control coating thickness.It is multiple with anti-sticker
After conjunction, it is placed in and 1h is dried in 60 DEG C of baking ovens, to required degree, cutting, sealing is obtained final product regulation water content after cooling.
The liquid dressing of embodiment 7
【Prescription】Raw material：Rehabilitation is new：0.4g garden burnet tannins 0.2g
Auxiliary material：Chitin 20g borneol 20g collagens 30g
After said medicine is well mixed with dressing, distilled water constant volume is added to be mixed to 100ml.First by chitin,
Collagen and distilled water heating for dissolving, heating-up temperature are 80~100 DEG C, add the blending of inorganic acid, formaldehyde and foaming agent and stir
Mix；By blend solution hot briquetting, the temperature of thermoforming is 60~90 DEG C, and the time of thermoforming is 4~12 hours；Finally by heat
The product of shaping is cleaned, and is obtained final product.
The foamed material patch of embodiment 8
【Prescription】Raw material：Rehabilitation is new：0.4g garden burnet tannins 0.2g
Auxiliary material：Chitin 20g polyvinyl alcohol 25g
First by chitin, polyvinyl alcohol and distilled water heating for dissolving, heating-up temperature is 80~100 DEG C, and medicine is mixed into equal
After even in the auxiliary material of addition melting, blending and stirring；By blend solution hot briquetting, the temperature of thermoforming is 60~90 DEG C, heat into
The time of type is 4~12 hours；Finally the product of thermoforming is cleaned, is obtained final product.
The film of embodiment 9
【Prescription】Raw material：Rehabilitation is new：0.4g garden burnet tannins 0.2g
Auxiliary material：Polyvinyl alcohol 30g borneol 20g glycerine 10g CMC-Na 15g
First by polyvinyl alcohol heating for dissolving in glycerine, heating-up temperature is 80~100 DEG C, and borneol is finely ground rear thin with medicine
Powder and CMC-Na are sufficiently mixed into after medicine slurry in the auxiliary material for adding melting, blending and stirring.Then with film applicator film into required
Thickness, the area of UD film is calculated after drying according to drug content, cut into the small lattice of single dose, packaging is obtained final product.
The pressure sensitive adhesive of embodiment 10
【Prescription】Raw material：Rehabilitation is new：0.4g garden burnet tannins 0.2g
Auxiliary material：SIS thermoplastic elastomer (TPE) 15g C5 resin 10g mineral oil 5g acrylic resin 5g polyethylene glycol
10g diphenylamines 3g
First pass through banburying mode carries out physical blending by SIS types thermoplastic elastomer (TPE), acrylic resin etc. in advance, in melting
Add medicine and other constituents well mixed prepared under state.
The American-cockroach-extract used in the present invention, meets in WS3-B-3674-2000 (Z) to " American cockroach Perip
Leneta ameri Cana dry the extract of polypide " limit standard (i.e. rehabilitation is new), i.e., according to dry product calculate, containing total
Amino acid must not be less than 7.0%.Its preparation method, referring to current rehabilitation is new or American-cockroach-extract preparation method, also
Following concrete operations can be used：
American cockroach 500g is taken, is crushed, extracted with 85% ethanol, add 7 times of amount alcohol refluxs to extract for the first time 2 hours,
Add 5 times of amount alcohol refluxs extraction 1.5 hours for second, third time plus 3 times are measured alcohol refluxs and extracted 1 hour, merging extract solution, mistake
Filter, centrifugation, 4000r/min, 10min, supernatant stands more than 8h, and filtration, filtrate recycling ethanol is simultaneously condensed into thick paste 100ml,
3000ml pure water is added, heating stirring stands, and cooling makes grease fully float, and removes clear liquid, filters, and filtrate is condensed into thick
Cream, obtains American-cockroach-extract (282g, about 300ml).
Garden burnet total tannin used in the present invention, refers to garden burnet extract part of the content of tannin more than 50%, in can using
Conventional tannin method for extraction and purification is prepared (such as in medicine chemistry or natural plants chemistry《Chemistry for Chinese Traditional Medicine》, Xiao Chonghou, Shanghai science
Technology publishing house, the 1st edition, page 573~574 in 1997), also can be using presently disclosed garden burnet tannin method for extraction and purification (such as
PCT/CN2011/079633；Yang Jinhui, causes murine interleukin to reduce disease shadow etc., the preparation of garden burnet tannin and its to endoxan
Loud Primary Study, Chinese Medicine biotechnology, 2 months 2013；Peng Cheng, garden burnet total tannin is extracted, purified and activity research, lucky
Woods university, 2012).
In order to pass through instance interpretation technical scheme, 85% garden burnet is not less than in the present invention using tannin purity
The preparation and experiment of product are carried out as a example by total tannin, its preparation method uses following steps：
Garden burnet medicinal material 200g is taken, it is coarse granule to crush, 10 times are measured 70% acetone ultrasonic extraction 2 times, each 1h, liquid filter
Cross, 45 DEG C of filtrate is recovered under reduced pressure acetone to complete, pours out concentrate, add water to 800mL, place 1h, filter off precipitation, collect filter
Liquid, is extracted 2 times with 3 times of water saturation ethyl acetate of amount, collects extract, and 45 DEG C are recovered under reduced pressure ethyl acetate to complete, pour out
Concentrate, puts 45 DEG C of drying with water baths to dry, 45 DEG C of vacuum drying 12h, obtains crude product tannin.Crude product tannin is taken, the 400ml that adds water is molten
Solution, places 1h, and filtration precipitation, filtrate is placed in evaporating dish, and (5g gelatin is dissolved in 150mL water slowly to spray into gelatin solution 150mL
In), 1h is placed, precipitation is collected, taken out after freeze-drying 12h, fine powder is ground to form, with 90% acetone 500ml ultrasonic extraction 2h, medicine
Acetone is recovered under reduced pressure in 45 DEG C to complete for liquid, pours out concentrate, and 45 DEG C of vacuum drying 12h obtain garden burnet total tannin.
The screening of the medication of embodiment 10 proportioning
1. material
1.1 tested material
(1) American cockroach pharmaceutical decocting piece.
(2) garden burnet pharmaceutical decocting piece.
(4) PVP PVPk30.
(5) the copolymer NP-700 of polyacrylic acid and Sodium Polyacrylate.
(6) azone.
(7) Dihydroxyaluminum Aminoacetate, tartaric acid.
2 pharmaceutical composition optimal proportions are screened
2.1 experiment materials, instrument
2.1.1 tested material
(1) American-cockroach-extract, prepares with reference to preceding method.
(2) garden burnet tannin extract, prepares with reference to preceding method.
2.1.2 chemical reagent
(1) 2% Nembutal sodium solution, compound method：2g yellow Jackets solids are accurately weighed with electronic balance, is loaded on
In beaker, 98ml physiological saline is added, stirring and dissolving is obtained final product.
(2) Iodophor.
(3) 70% ethanol for disinfection
(4) 8% vulcanized sodium-ethanol solution：Compound method：8gNa is accurately weighed with electronic balance2S solids, loaded on burning
In cup, the ethanol of 100ml70%, stirring and dissolving machine is added to obtain.
(5) physiological saline.
2.3 experimental animal
SD rats：185~245g of body weight, is provided, experimental animal credit number by Chengdu up to large bio tech ltd：
scxkc(111)2008-24。
2.4 experimental site
Experimental site is Chengdu University of Traditional Chinese Medicine of State Administration of Traditional Chinese Medicine herbal pharmacology scientific research three-level laboratory
(NO.TCM-2009-315)。
2.5 experimental animal feeding facilities and condition
Experimental animal observation ward of pharmaceutical college of Chengdu University of Traditional Chinese Medicine, experimental animal uses credit number SYXK (river) 2009-
124.Indoor illumination, humidity, proper temperature (airconditioning control), ventilation condition are good, and environment is relatively quiet, and daily illumination 12 is small
When, per 5, cage.
2.6 laboratory apparatus
(1) BS-600L electronic balances：Specification：600g/0.1g, Shanghai Yousheng Balance Co., Ltd..
(2) electric-heated thermostatic water bath：Model SK12-6, Ningbo of Zhejiang medical apparatus and instruments factory.
(3) counterweight.
3 statistical methods
Statistical analysis is carried out with the softwares of SPSS 17.0.Data are represented with mean ± standard deviation (x ± s), using Dan Yin between group
LSD inspections are carried out between plain variance analysis, the neat person's group of variance, heterogeneity of variance person carries out Tamhane ' s T2 inspections.
4 experimental techniques
4.1 pairs of influence experiments of After Scalding In Rat skin
4.1.1 drug ratio
Fully and effectively to carry out the screening of optimal drug compatibility and optimum dose proportion relation to primary election prescription, and as far as possible
Test number (TN) is reduced, this experiment carries out packet design using uniform design.We are made up of 2 kinds of Chinese medical extracts, according to medicine
Thing attribute and empirical dose relation, take same dose, take experimental design and the evaluation of drug regimen and dosage ratio research
Method, with 0.2g as downward gradient, while separately setting blank control group and positive drug control group.The tool of each dosage ratio group medicine
Body proportionate relationship is shown in Table 1.
1 two kinds of extract ratios of table
4.1.2 medicine efficacy screening
200 SPF grades of SD rats are randomly divided into by body weight：Model group (MD groups), positive group (Yunnan Baiyao group) composition 1
~18 groups, every group 10, male and female half and half.
4.1.2.1 rat incised injury modelling
(1) preoperative medication
Experimental rat presses the yellow Jackets normal saline solution of 30mg/kg dosage intraperitoneal injection 2%, anesthesia.
(2) incised injury wound model modeling：
It is fixed after Animal Anesthesia, the circle of diameter 1.5cm is drawn in dorsal midline, after border circular areas are sterilized with 70% cotton ball soaked in alcohol
Cut off round inner skin.
4.1.2.2 method of administration
The covering of model group physiological saline gauze compares group, and positive group is used and speckles with Yunnan Baiyao normal saline suspension
Sterile gauze covering, (two kinds of compositions are total for the medicine preparing normal saline suspension of drug ratio group different proportion of the present invention
Concentration 0.2g/ml) sterile gauze application.Test group of animals used is wrapped up with 5 layers of sterile gauze.
4.1.2.3 animal care
Since the modeling same day：Model group changes physiological saline gauze, and positive group changes Yunnan Baiyao gauze, drug ratio
Group changes pharmaceutical gauze, continuous 14 days.
4.1.3 observation of curative effect
(1) bleeding stopping period：The timing since first administration, to the surface of a wound stops bleeding, records the bleeding time.
(2) wound healing time：Wound healing time：During the complete epithelialization of the surface of a wound, wound healing time is calculated.
(3) Wound healing rate：Face was calculated with computer image analysis software to each experimental group surface of a wound respectively in postoperative 14 days
Product, determines the healing percentage of each group surface of a wound.
Formula is：Wound healing rate (%)=[(original surface of a wound area-do not heal surface of a wound area)/original surface of a wound area] ×
100%
5 experimental results
5.1 each experimental group bleeding stopping period comparative results are shown in Table 2.
Each experimental group bleeding stopping period (min) of table 2
Note：Compare with model group,**P<0.01,*P<0.05；Compare with Yunnan Baiyao group,△△P<0.01,△P<0.05
As shown in Table 2, compare with model group, each experimental group bleeding stopping period is substantially reduced (P<0.05), wherein, combination
Thing 8,9,11 has pole to be substantially reduced (P<0.01)；Compare with Yunnan Baiyao group, 9,11 groups of rat bleeding stopping periods of composition have
It is substantially reduced (P<0.05), 8 groups of composition has pole to be substantially reduced (P<0.01).
5.2 each experimental group wound healing time comparative results are shown in Table 3.
Each experimental group wound healing time (d) of table 3
Note：Compare with model group,**P<0.01,*P<0.05；Compare with Yunnan Baiyao group,△△P<0.01,△P<0.05
As shown in Table 3, compare with model group, each experimental group wound healing time is substantially reduced (P<0.05), wherein,
8 groups of composition has pole to be substantially reduced (P<0.01)；Compare with Yunnan Baiyao group, 9,11 groups of Rat Wound Healing times of composition are equal
It is substantially reduced (P<0.05), 7,8 and 11 groups of composition is substantially reduced (P<0.05).
5.2 each experimental group Wound healing rate comparative results are shown in Table 4.
Each experimental group Wound healing rate (%) of table 4
Note：Compare with model group,**P<0.01,*P<0.05；Compare with Yunnan Baiyao group,△△P<0.01,△P<0.05
As shown in Table 4, compare with model group, each experimental group Wound healing rate has pole to significantly increase (P<0.01)；With cloud
Southern baiyao group compares, and composition 6 and 8 groups of Rat Wound Healing rates significantly increase (P<0.05).
6 experiment conclusions
American-cockroach-extract and the various comparative compositions of garden burnet tannin extract are respectively provided with good to rat incised injury
Therapeutic action, is mainly reflected in：The incised injury rat bleeding time can be shortened, with good anastalsis；Can shorten and cut
Incised wound Rat Wound Healing time, raising Wound healing rate, wherein with pharmaceutical composition 8, i.e. American-cockroach-extract:Garden burnet tan
Matter extract=0.4g:0.2g has optimum efficiency.
Claims (10)
1. the use of American-cockroach-extract and Radix Sangusorbae extract in external application hemostasis, the combination medicine of wound healing is prepared
On the way；The weight proportion of each bulk drug is in the combination medicine：2~6 parts of American-cockroach-extract, Radix Sangusorbae extract 1~6
Part；
The American-cockroach-extract is the extract that American cockroach Perip leneta ameri Cana dry polypide, containing total
Amino acid is no less than 7.0%；The Radix Sangusorbae extract be garden burnet total tannin, in the garden burnet total tannin content of tannin 50% with
On.
2. purposes according to claim 1, it is characterised in that：The weight proportion of each bulk drug in the combination medicine
For：4 parts of American-cockroach-extract, 2~6 parts of Radix Sangusorbae extract.
3. purposes according to claim 2, it is characterised in that：The weight proportion of each bulk drug in the combination medicine
For：4 parts of American-cockroach-extract, Radix Sangusorbae extract 2 or 6 parts.
4. purposes according to claim 1, it is characterised in that：Content of tannin is more than 80% in the garden burnet total tannin.
5. purposes according to claim 4, it is characterised in that：Content of tannin is more than 85% in the garden burnet total tannin.
6. a kind of pharmaceutical composition, it is characterised in that：It is the preparation being prepared from by the bulk drug of following weight proportion：
1~10 part of American-cockroach-extract, 1~10 part of Radix Sangusorbae extract；
The American-cockroach-extract is the extract that American cockroach Perip leneta ameri Cana dry polypide, containing total
Amino acid is no less than 7.0%；The Radix Sangusorbae extract be garden burnet total tannin, in the garden burnet total tannin content of tannin 50% with
On.
7. pharmaceutical composition according to claim 6, it is characterised in that：The preparation is external preparation.
8. pharmaceutical composition according to claim 7, it is characterised in that：The external preparation be patch, dressing, film,
Adhesive.
9. pharmaceutical composition according to claim 8, it is characterised in that：The patch is hydrogel patch, foamed material
Patch；The adhesive is pressure sensitive adhesive.
10. claim 6~9 any one described pharmaceutical composition is preparing hemostasis, the use in the medicine of wound healing
On the way.
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| CN111118734B (en) * | 2020-01-16 | 2021-06-01 | 东莞华工创为生物科技有限公司 | Polyvinyl alcohol/carboxymethyl chitosan nanofiber medical dressing and preparation method and application thereof |
| CN115518026A (en) * | 2021-06-25 | 2022-12-27 | 四川好医生攀西药业有限责任公司 | External veterinary drug containing periplaneta americana as well as preparation method and application thereof |
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