CN104288278A - New use of Periplaneta americana and Radix Sanguisorbae in combination drug - Google Patents

New use of Periplaneta americana and Radix Sanguisorbae in combination drug Download PDF

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CN104288278A
CN104288278A CN201410326015.7A CN201410326015A CN104288278A CN 104288278 A CN104288278 A CN 104288278A CN 201410326015 A CN201410326015 A CN 201410326015A CN 104288278 A CN104288278 A CN 104288278A
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extract
radix sanguisorbae
periplaneta americana
tannin
preparation
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CN104288278B (en
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苏柘僮
熊永爱
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Sichuan Good Doctor Panxi Pharmaceutical Co., Ltd.
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CHENGDU BAICAO HEJI TECHNOLOGY Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/739—Sanguisorba (burnet)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
    • A61K9/7007—Drug-containing films, membranes or sheets
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
    • A61K9/7023—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0061—Use of materials characterised by their function or physical properties
    • A61L26/0066—Medicaments; Biocides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents

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Abstract

The invention provides a use of Periplaneta americana or its extract and Radix Sanguisorbae or its extract in the preparation of an external bleeding stopping and wound healing promoting combination drug. The combination drug comprises, by weight, 1-10 parts of Periplaneta americana or its extract and 1-10 parts of Radix Sanguisorbae or its extract. The combination use of Periplaneta americana and Radix Sanguisorbae in the invention significantly increases the wound restoration effect and the bleeding stopping effect of drugs, have same or even better effects than a classic drug Yunnan Baiyao, and provides a new choice for the clinic drug use.

Description

The drug combination novelty teabag of periplaneta americana and Radix Sanguisorbae
Technical field
The present invention relates to the drug combination novelty teabag of periplaneta americana and Radix Sanguisorbae.
Background technology
Skin is the maximum organ of human body, carries the function of protection health, perspire, sensible heat cold-peace pressure.Skin covers whole body, and it to make in body various tissue and organ from the invasion and attack of physical property, mechanicalness, chemical and pathogenic microorganism.Through being scratched, wound, burn and to fester etc. and cause skin damage after, be easy to cause bacteriological infection, body fluid runs off and causes various complication.Be foundation according to wet union theory, modern treatment of wounds all takes moisture treatment, is wound and builds a microenvironment being beneficial to tissue growth (micro-acid, anaerobic or hypoxia, appropriateness moistening).Prepare can effectively stop blooding, the medicine of wound healing seems particularly important.
Periplaneta americana Perip leneta ameri Cana is the insecticide that in Blattidae, volume is maximum.Research finds, American-cockroach-extract has good repair in trauma effect.As Kangfuxin Liquid, it is prepared from by the extract (namely rehabilitation is new) of the dry polypide of periplaneta americana Perip leneta ameri Cana, in American-cockroach-extract, calculate according to dry product, 7.0% (see WS3-B-3674-2000 (Z)) must not be less than containing total amino acids.Kangfuxin Liquid can promoting blood circulation, nourishing yin and promoting granulation.For oral administration: for stagnation of blood stasis, to have a stomachache hemorrhage, taste-blindness rate; And deficiency of YIN pulmonary tuberculosis, phthisical auxiliary treatment.External: for the wound surface of incised wound, wound, ulcer, fistula, burn, scald, decubital ulcer.
Yet there are no the report by periplaneta americana and Radix Sanguisorbae conbined usage.
Summary of the invention
The object of the present invention is to provide the drug combination novelty teabag of periplaneta americana and Radix Sanguisorbae.Present invention also offers a kind of medical composition and its use.
The invention provides periplaneta americana or its extract and Radix Sanguisorbae or the purposes of its extract in the combination medicine preparing external hemostasis, wound healing.
Wherein, in described combination medicine, the weight proportion of each crude drug is: periplaneta americana or its extract 1 ~ 10 part, Radix Sanguisorbae or its extract 1 ~ 10 part.
Further, in described combination medicine, the weight proportion of each crude drug is: periplaneta americana or its extract 1 ~ 6 part, Radix Sanguisorbae or its extract 1 ~ 6 part.
Further, in described combination medicine, the weight proportion of each crude drug is: periplaneta americana or its extract 2 ~ 6 parts, Radix Sanguisorbae or its extract 1 ~ 6 part.
Preferably, in described combination medicine, the weight proportion of each crude drug is: periplaneta americana or its extract 4 parts, Radix Sanguisorbae or its extract 2 ~ 6 parts.
Further preferably, in described combination medicine, the weight proportion of each crude drug is: periplaneta americana or its extract 4 parts, Radix Sanguisorbae or its extract 2 or 6 parts.
Wherein, described American-cockroach-extract is the extract of the dry polypide of periplaneta americana Perip leneta ameri Cana, is no less than 7.0% containing total amino acids; Described Radix Sangusorbae extract is Radix Sanguisorbae total tannin, and in described Radix Sanguisorbae total tannin, content of tannin is more than 50%.
American-cockroach-extract of the present invention, meet the limit standard to " extract of the dry polypide of periplaneta americana Perip leneta ameri Cana " in WS3-B-3674-2000 (Z), calculate according to dry product, must not be less than 7.0% containing total amino acids, the rehabilitation as commercially available is new.In addition, American-cockroach-extract preparation method common at present also can be used, such as:
Get periplaneta americana, pulverize, 70 ~ 90%v/v ethanol extraction, merge extractive liquid, after reclaiming ethanol, add water mixing, heated and stirred, leaves standstill, and cooling, makes oils and fats fully float, take off clear liquid, concentrates or namely obtain American-cockroach-extract after drying.
Concentration of alcohol can select 80 ~ 90%v/v, such as 80%v/v, 81%v/v, 82%v/v, 83%v/v, 84%v/v, 85%v/v, 86%v/v, 87%v/v, 88%v/v, 89%v/v, 90%v/v.
Radix Sanguisorbae of the present invention is the dry root of rosaceous plant Radix Sanguisorbae Sanguisorba officinalis L. or Radix Sanguisorbae Sanguisorba officinalis L.var.longifolia (Bert.) the Yu et Li that comes into leaves.
According to the relevant regulations of natural plants chemical field, total polysaccharides, total flavones, total alkaloids, total tannin etc. refer in extract respectively containing the extract part more than 50% such as polysaccharide, flavone, alkaloid, tannin.The Radix Sanguisorbae total tannin used in the present invention, meet above-mentioned relevant regulations equally, wherein the content of tannin is more than 50%, can select the Radix Sanguisorbae total tannin of content more than 80% further, and such as the present invention is used for the Radix Sanguisorbae total tannin of illustrational content more than 85%.
The preparation of Radix Sanguisorbae total tannin, adopts water, ethanol, aquiferous ethanol, acetone or aqueous acetone to be that solvent extracts usually, then carries out polishing purification through the method such as combination of chromatography, precipitation of protein, solvent method or said method.
Wherein, described chromatography can adopt gel, macroporous adsorbent resin adsorbs.
Described solvent method, refer to by containing total tannin crude product aqueous degreaser process after, then with ethyl acetate extract, obtain Radix Sanguisorbae tannin extract; Or total tannin crude product is dissolved in ethanol and ethyl acetate, the Precipitation Radix Sanguisorbae tannin that adds diethyl ether extract.
Described precipitation of protein, adopts the method precipitated with the gelatin more, can be gelatin precipitation acetone analytic method.
Solvent method and gelatin precipitation acetone method can be adopted in the present invention to combine preparation for Radix Sanguisorbae total tannin, and its operating procedure is as follows:
(1) get Radix Sanguisorbae medical material, pulverize, add aqueous acetone and extract, collect extracting solution, after reclaiming acetone, add water mixing, staticly settles, get supernatant, be extracted with ethyl acetate, and collects extraction into ethyl acetate layer, reclaims ethyl acetate, obtain tannin crude product;
(2) get tannin crude product, be dissolved in water, get supernatant and add gelatin solution, staticly settle, after getting precipitation drying, dissolve by acetone or aqueous acetone, collect supernatant, reclaim acetone, dry, obtain Radix Sanguisorbae total tannin.
Wherein, the aqueous acetone concentration of step (1) is 60-80%v/v, be preferably 65-75%v/v, as 65%v/v, 66%v/v, 67%v/v, 68%v/v, 69%v/v, 70%v/v, 71%v/v, 72%v/v, 73%v/v, 74%v/v, 75%v/v etc.
In step (2), aqueous acetone concentration is 80 ~ 95%v/v, be preferably 85-95%v/v, such as 85%v/v, 86%v/v, 87%v/v, 88%v/v, 89%v/v, 90%v/v, 91%v/v, 92%v/v, 93%v/v, 94%v/v, 95%v/v etc.
Present invention also offers a kind of pharmaceutical composition, it is the preparation be prepared from by the crude drug of following weight proportion: periplaneta americana or its extract 1 ~ 10 part, Radix Sanguisorbae or its extract 1 ~ 10 part.
Further, it is the preparation be prepared from by the crude drug of following weight proportion: periplaneta americana or its extract 1 ~ 6 part, Radix Sanguisorbae or its extract 1 ~ 6 part.
Further, it is the preparation be prepared from by the crude drug of following weight proportion: periplaneta americana or its extract 2 ~ 6 parts, Radix Sanguisorbae or its extract 1 ~ 6 part.
Preferably, it is the preparation be prepared from by the crude drug of following weight proportion: periplaneta americana or its extract 4 parts, Radix Sanguisorbae or its extract 2 ~ 6 parts.
Further preferably, it is the preparation be prepared from by the crude drug of following weight proportion: periplaneta americana or its extract 4 parts, Radix Sanguisorbae or its extract 2 or 6 parts.
Wherein, described preparation is external preparation.
Further, described external preparation is patch, dressing, membrane, adhesive.
Further, described patch is hydrogel patch, foamed materials patch; Described adhesive is pressure sensitive adhesive.
Present invention also offers the purposes of aforementioned pharmaceutical compositions in the medicine of preparation hemostasis, wound healing.
Further, described medicine is external used medicine.
The present invention, by after periplaneta americana and Radix Sanguisorbae conbined usage, significantly improves medicine to the repair of wound surface and anastalsis, and drug effect and classical medicine YUNNAN BAIYAO are quite, even better, for clinical application provides new selection.
Detailed description of the invention
Embodiment 1 hydrogel patch
[prescription] raw material: American-cockroach-extract: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: aluminum dihydroxyamino acetate 10g NP-70030g glycerol 30g
Borneolum Syntheticum 20g PVP k30 10g
[method for making] is with in the 1000mL glass flask of intense stirring device, the medicine of recipe quantity and cross-linking agent aluminum dihydroxyamino acetate are dissolved in the mixed solution of wetting agent glycerol, penetrating agent and purified water, add the hydrogel based material NP-700 of recipe quantity, abundant stirring, makes its mix homogeneously; Again the pH adjusting agent tartaric acid of the recipe quantity of mix homogeneously, tackifier PVP k30 and water for injection are added wherein, stir, make its mix homogeneously.Again hydrogel based for above-mentioned pastille material is adopted doctor blade process, be coated on the non-woven fabrics of hydrophobization process, controlling coating thickness is (1.5 ± 0.1) mm.After anti-sticker compound, be placed in 60 DEG C of dry 1h of baking oven, regulate water content to required degree, cut after cooling, seal and get final product.
Embodiment 2 liquid dressing
[prescription] raw material: American-cockroach-extract: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: chitin 20g Borneolum Syntheticum 20g collagen protein 30g
After said medicine is mixed homogeneously with dressing, add distilled water standardize solution and mix to 100ml.First by chitin, collagen protein and distilled water heating for dissolving, heating-up temperature is 80 ~ 100 DEG C, then adds mineral acid, formaldehyde and foaming agent blending and stirring; By blend solution hot briquetting, the temperature of thermoforming is 60 ~ 90 DEG C, and the time of thermoforming is 4 ~ 12 hours; Finally the product of thermoforming is cleaned, to obtain final product.
Embodiment 3 foamed materials patch
[prescription] raw material: American-cockroach-extract: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: chitin 20g polyvinyl alcohol 25g
First by chitin, polyvinyl alcohol and distilled water heating for dissolving, heating-up temperature is 80 ~ 100 DEG C, adds in the adjuvant of melting, blending and stirring after medicament mixed is even; By blend solution hot briquetting, the temperature of thermoforming is 60 ~ 90 DEG C, and the time of thermoforming is 4 ~ 12 hours; Finally the product of thermoforming is cleaned, to obtain final product.
Embodiment 4 membrane
[prescription] raw material: American-cockroach-extract: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: polyvinyl alcohol 30g Borneolum Syntheticum 20g glycerol 10g CMC-Na15g
First by polyvinyl alcohol heating for dissolving in glycerol, heating-up temperature is 80 ~ 100 DEG C, adds in the adjuvant of melting, blending and stirring by fully mixing after patent medicine is starched with fine drug powder and CMC-Na after Borneolum Syntheticum porphyrize.Then the thickness required for becoming with film applicator film, calculates the area of unit dose film, cuts into the little lattice of single dose, pack and get final product according to drug content after oven dry.
Embodiment 5 pressure sensitive adhesive
[prescription] raw material: American-cockroach-extract: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: SIS thermoplastic elastomer (TPE) 15g C5 resin 10g mineral oil 5g third
Olefin(e) acid resin 5g Polyethylene Glycol 10g diphenylamines 3g
First in advance SIS type thermoplastic elastomer (TPE), acrylic resin etc. are carried out physical blending by banburying mode, add medicine and other constituent mix homogeneously in the molten state and obtain.
Embodiment 6 hydrogel patch
[prescription] raw material: rehabilitation is new: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: aluminum dihydroxyamino acetate 10g NP-70030g glycerol 30g
Borneolum Syntheticum 20g PVP k30 10g
[method for making] is with in the 1000mL glass flask of intense stirring device, the medicine of recipe quantity and cross-linking agent aluminum dihydroxyamino acetate are dissolved in the mixed solution of wetting agent glycerol, penetrating agent and purified water, add the hydrogel based material NP-700 of recipe quantity, abundant stirring, makes its mix homogeneously; Again the pH adjusting agent tartaric acid of the recipe quantity of mix homogeneously, tackifier PVP k30 and water for injection are added wherein, stir, make its mix homogeneously.Again hydrogel based for above-mentioned pastille material is adopted doctor blade process, be coated on the non-woven fabrics of hydrophobization process, controlling coating thickness is (1.5 ± 0.1) mm.After anti-sticker compound, be placed in 60 DEG C of dry 1h of baking oven, regulate water content to required degree, cut after cooling, seal and get final product.
Embodiment 7 liquid dressing
[prescription] raw material: rehabilitation is new: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: chitin 20g Borneolum Syntheticum 20g collagen protein 30g
After said medicine is mixed homogeneously with dressing, add distilled water standardize solution and mix to 100ml.First by chitin, collagen protein and distilled water heating for dissolving, heating-up temperature is 80 ~ 100 DEG C, then adds mineral acid, formaldehyde and foaming agent blending and stirring; By blend solution hot briquetting, the temperature of thermoforming is 60 ~ 90 DEG C, and the time of thermoforming is 4 ~ 12 hours; Finally the product of thermoforming is cleaned, to obtain final product.
Embodiment 8 foamed materials patch
[prescription] raw material: rehabilitation is new: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: chitin 20g polyvinyl alcohol 25g
First by chitin, polyvinyl alcohol and distilled water heating for dissolving, heating-up temperature is 80 ~ 100 DEG C, adds in the adjuvant of melting, blending and stirring after medicament mixed is even; By blend solution hot briquetting, the temperature of thermoforming is 60 ~ 90 DEG C, and the time of thermoforming is 4 ~ 12 hours; Finally the product of thermoforming is cleaned, to obtain final product.
Embodiment 9 membrane
[prescription] raw material: rehabilitation is new: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: polyvinyl alcohol 30g Borneolum Syntheticum 20g glycerol 10g CMC-Na15g
First by polyvinyl alcohol heating for dissolving in glycerol, heating-up temperature is 80 ~ 100 DEG C, adds in the adjuvant of melting, blending and stirring by fully mixing after patent medicine is starched with fine drug powder and CMC-Na after Borneolum Syntheticum porphyrize.Then the thickness required for becoming with film applicator film, calculates the area of unit dose film, cuts into the little lattice of single dose, pack and get final product according to drug content after oven dry.
Embodiment 10 pressure sensitive adhesive
[prescription] raw material: rehabilitation is new: 0.4g Radix Sanguisorbae tannin 0.2g
Adjuvant: SIS thermoplastic elastomer (TPE) 15g C5 resin 10g mineral oil 5g third
Olefin(e) acid resin 5g Polyethylene Glycol 10g diphenylamines 3g
First in advance SIS type thermoplastic elastomer (TPE), acrylic resin etc. are carried out physical blending by banburying mode, add medicine and other constituent mix homogeneously in the molten state and obtain.
The American-cockroach-extract used in the present invention, meet the limit standard (namely rehabilitation is new) to " extract of the dry polypide of periplaneta americana Perip leneta ameri Cana " in WS3-B-3674-2000 (Z), namely calculate according to dry product, must not 7.0% be less than containing total amino acids.Its preparation method, the new or American-cockroach-extract preparation method see current rehabilitation, also can use following concrete operations:
Get periplaneta americana 500g, pulverize, with the ethanol extraction of 85%, first time adds 7 times amount alcohol reflux 2 hours, second time adds 5 times amount alcohol reflux 1.5 hours, third time adds 3 times amount alcohol reflux 1 hour, merge extractive liquid, filter, centrifugal, 4000r/min, 10min, supernatant leaves standstill more than 8h, filter, filtrate recycling ethanol is also condensed into thick paste 100ml, add 3000ml pure water, heated and stirred, leave standstill, cooling, oils and fats is fully floated, take off clear liquid, filter, filtrate is condensed into thick paste, obtain American-cockroach-extract (282g, about 300ml).
Radix Sanguisorbae total tannin used in the present invention, refer to the Radix Sanguisorbae extract part of content of tannin more than 50%, tannin method for extraction and purification preparation conventional in Chemistry for Chinese Traditional Medicine or natural plants chemistry can be adopted (as " Chemistry for Chinese Traditional Medicine ", Xiao Chonghou, Shanghai science tech publishing house, 1997 the 1st edition, 573 ~ 574 pages), also can adopt at present disclosed Radix Sanguisorbae tannin method for extraction and purification (as PCT/CN2011/079633; Yang Jinhui, etc. the preparation of, Radix Sanguisorbae tannin and cause the preliminary study that murine interleukin reduces disease impact, Chinese Medicine biotechnology, in February, 2013 to cyclophosphamide; Peng Cheng, the extraction of Radix Sanguisorbae total tannin, purification and activity research, Jilin University, 2012).
In order to by instance interpretation technical scheme of the present invention, the Radix Sanguisorbae total tannin using tannin purity to be not less than 85% in the present invention carries out preparation and the test of product for example, and its preparation method adopts following steps:
Get Radix Sanguisorbae medical material 200g, pulverize as coarse granule, 10 times amount 70% acetone supersound extraction 2 times, each 1h, medicinal liquid filters, and filtrate 45 DEG C of reclaim under reduced pressure acetone are to complete, pour out concentrated solution, add water to 800mL, place 1h, elimination precipitates, and collects filtrate, with the water saturation extraction into ethyl acetate 2 times of 3 times amount, collect extract, 45 DEG C of reclaim under reduced pressure ethyl acetate, to completely, pour out concentrated solution, put 45 DEG C of drying with water baths to dry, 45 DEG C of vacuum drying 12h, obtain crude product tannin.Get crude product tannin, the 400ml that adds water dissolves, and places 1h, filter precipitation, filtrate is placed in evaporating dish, slowly sprays into gelatin solution 150mL (5g gelatin is dissolved in 150mL water), place 1h, collecting precipitation, takes out after lyophilization 12h, grind to form fine powder, with 90% acetone 500ml supersound extraction 2h, medicinal liquid, in 45 DEG C of reclaim under reduced pressure acetone to completely, pours out concentrated solution, 45 DEG C of vacuum drying 12h, obtain Radix Sanguisorbae total tannin.
The screening of embodiment 10 medication proportioning
1. material
1.1 tested material
(1) periplaneta americana pharmaceutical decocting piece.
(2) Radix Sanguisorbae pharmaceutical decocting piece.
(4) polyvinyl pyrrolidone PVPk30.
(5) the copolymer NP-700 of polyacrylic acid and sodium polyacrylate.
(6) azone.
(7) Dihydroxyaluminum Aminoacetate, tartaric acid.
2 pharmaceutical composition optimal proportion screenings
2.1 experiment materials, instrument
2.1.1 tested material
(1) American-cockroach-extract, with reference to preceding method preparation.
(2) Radix Sanguisorbae tannin extract, with reference to preceding method preparation.
2.1.2 chemical reagent
(1) 2% Nembutal sodium solution, compound method: accurately take 2g pentobarbital sodium solid with electronic balance, be loaded in beaker, add 98ml normal saline, stirring and dissolving and get final product.
(2) povidone iodine.
(3) 70% ethanol for disinfection
Sodium sulfide-the alcoholic solution of (4) 8%: compound method: accurately take 8gNa with electronic balance 2s solid, is loaded in beaker, adds the ethanol of 100ml70%, and stirring and dissolving machine obtains.
(5) normal saline.
2.3 laboratory animal
SD rat: body weight 185 ~ 245g, by Chengdu, Da Shuo bio tech ltd provides, laboratory animal credit number: scxkc (111) 2008-24.
2.4 experimental site
Experimental site is herbal pharmacology section of Chengdu University of Traditional Chinese Medicine of State Administration of Traditional Chinese Medicine Kenzo level laboratory (NO.TCM-2009-315).
2.5 experimental animal feeding facility and conditions
Laboratory animal observation ward of pharmaceutical college of Chengdu University of Traditional Chinese Medicine, laboratory animal occupancy permit SYXK (river) 2009-124.Indoor illumination, humidity, proper temperature (airconditioning control), ventilation condition is good, environment facies to peace and quiet, illumination every day 12 hours, 5, every cage.
2.6 experimental apparatus
(1) BS-600L electronic balance: specification: 600g/0.1g, Shanghai Yousheng Balance Co., Ltd..
(2) electric-heated thermostatic water bath: model SK12-6, Ningbo of Zhejiang medical apparatus and instruments factory.
(3) counterweight.
3 statistical method
Statistical analysis is carried out with SPSS17.0 software.Data are with mean ± standard deviation represent, adopt one factor analysis of variance between group, carry out LSD inspection between the neat person's group of variance, heterogeneity of variance person carries out Tamhane ' s T2 and checks.
4 experimental techniques
4.1 test the impact of After Scalding In Rat skin
4.1.1 drug ratio
For carrying out the screening of optimal drug compatibility and optimum dose proportion relation fully and effectively to primary election prescription, and reduce test number (TN), this test adopts uniform design to carry out packet design as far as possible.We are made up of 2 kinds of Chinese medicine extract, according to medicine attribute and empirical dose relation, all get same dose, take EXPERIMENTAL DESIGN and the evaluation methodology of drug regimen and dosage proportion research, take 0.2g as downward gradient, separately establish blank group and positive drug control group simultaneously.The concrete proportionate relationship of each dosage ratio group medicine is in table 1.
Table 1 two kinds of extract ratios
4.1.2 medicine efficacy screening
200 SPF level SD rats are divided at random by body weight: model group (MD group), positive group of (YUNNAN BAIYAO group) compositions 1 ~ 18 group, often organize 10, male and female half and half.
4.1.2.1 the modelling of rat incision
(1) preoperative medication
Experimental rat presses 30mg/kg dosage lumbar injection 2% pentobarbital sodium normal saline solution, anesthesia.
(2) incision wound model modeling:
Fixing after Animal Anesthesia, draw the circle of diameter 1.5cm in dorsal midline, border circular areas is to cut off skin in circle after 70% cotton ball soaked in alcohol sterilization.
4.1.2.2 route of administration
Model group normal saline gauze covers and compares group, positive group uses the sterile gauze speckling with YUNNAN BAIYAO normal saline suspension to cover, and the sterile gauze of the Pharmaceutical formulations normal saline suspension (two kinds of composition total concentration 0.2g/ml) of drug ratio group different proportion of the present invention is applied ointment or plaster.Test group of animals used is all with 5 layers of sterile gauze wrapping.
4.1.2.3 animal care
From modeling same day: model group changes normal saline gauze, positive group changed YUNNAN BAIYAO gauze, and drug ratio group changes pharmaceutical gauze, continuous 14 days.
4.1.3 observation of curative effect
(1) bleeding stopping period: timing from first administration, to wound surface stopping is hemorrhage, the record bleeding time.
(2) wound healing time: wound healing time: during the complete epithelization of wound surface, calculates wound healing time.
(3) Wound healing rate: in postoperative 14 days respectively to each experimental group wound surface computer image analysis software reference area, measures the healing percentage rate respectively forming face.
Formula is: Wound healing rate (%)=[(original wound surface area-do not heal wound surface area)/original wound surface area] × 100%
5 experimental results
5.1 each experimental group bleeding stopping period comparative results are in table 2.
The each experimental group bleeding stopping period (min) of table 2
Note: compare with model group, *p<0.01, *p<0.05; Compare with YUNNAN BAIYAO group, △ △p<0.01, △p<0.05
As shown in Table 2, compare with model group, each experimental group bleeding stopping period all has remarkable reduction (P<0.05), and wherein, compositions 8,9,11 all has extremely significantly reduction (P<0.01); Compare with YUNNAN BAIYAO group, compositions 9,11 groups of rat bleeding stopping period all have remarkable reduction (P<0.05), and compositions 8 groups has extremely significantly reduction (P<0.01).
5.2 each experimental group wound healing time comparative results are in table 3.
Each experimental group wound healing time (d) of table 3
Note: compare with model group, *p<0.01, *p<0.05; Compare with YUNNAN BAIYAO group, △ △p<0.01, △p<0.05
As shown in Table 3, compare with model group, each experimental group wound healing time all has remarkable reduction (P<0.05), and wherein, compositions 8 groups has extremely significantly reduction (P<0.01); Compare with YUNNAN BAIYAO group, all there are remarkable reduction (P<0.05) 9,11 groups of Rat Wound Healing times of compositions, and compositions 7,8 and 11 groups all has remarkable reduction (P<0.05).
5.2 each experimental group Wound healing rate comparative results are in table 4.
The each experimental group Wound healing rate (%) of table 4
Note: compare with model group, *p<0.01, *p<0.05; Compare with YUNNAN BAIYAO group, △ △p<0.01, △p<0.05
As shown in Table 4, compare with model group, each experimental group Wound healing rate all has pole to enlarge markedly (P<0.01); Compare with YUNNAN BAIYAO group, compositions 6 and 8 groups of Rat Wound Healing rates all enlarge markedly (P<0.05).
6 experiment conclusion
American-cockroach-extract and the various comparative compositions of Radix Sanguisorbae tannin extract all have good therapeutical effect to rat incision, are mainly reflected in: all can shorten the incision rat bleeding time, have good anastalsis; Equal can shorten the incision Rat Wound Healing time, improve Wound healing rate, wherein with pharmaceutical composition 8, i.e. American-cockroach-extract: Radix Sanguisorbae tannin extract=0.4g:0.2g has optimum efficiency.

Claims (10)

1. periplaneta americana or its extract and Radix Sanguisorbae or the purposes of its extract in the combination medicine preparing external hemostasis, wound healing; In described combination medicine, the weight proportion of each crude drug is:
Periplaneta americana or its extract 1 ~ 10 part, Radix Sanguisorbae or its extract 1 ~ 10 part.
2. purposes according to claim 1, is characterized in that: in described combination medicine, the weight proportion of each crude drug is: periplaneta americana or its extract 1 ~ 6 part, Radix Sanguisorbae or its extract 1 ~ 6 part;
Further, in described combination medicine, the weight proportion of each crude drug is: periplaneta americana or its extract 2 ~ 6 parts, Radix Sanguisorbae or its extract 1 ~ 6 part.
3. purposes according to claim 1 and 2, is characterized in that: in described combination medicine, the weight proportion of each crude drug is: periplaneta americana or its extract 4 parts, Radix Sanguisorbae or its extract 2 ~ 6 parts;
Further, in described combination medicine, the weight proportion of each crude drug is: periplaneta americana or its extract 4 parts, Radix Sanguisorbae or its extract 2 or 6 parts.
4. the purposes according to claims 1 to 3 any one, is characterized in that: described American-cockroach-extract is the extract of the dry polypide of periplaneta americana Perip leneta ameri Cana, is no less than 7.0% containing total amino acids; Described Radix Sangusorbae extract is Radix Sanguisorbae total tannin, and in described Radix Sanguisorbae total tannin, content of tannin is more than 50%.
5. purposes according to claim 4, is characterized in that: in described Radix Sanguisorbae total tannin, content of tannin is more than 80%, preferably more than 85%.
6. a pharmaceutical composition, is characterized in that: it is the preparation be prepared from by the crude drug of following weight proportion:
Periplaneta americana or its extract 1 ~ 10 part, Radix Sanguisorbae or its extract 1 ~ 10 part.
7. pharmaceutical composition according to claim 6, is characterized in that: described preparation is external preparation.
8. pharmaceutical composition according to claim 7, is characterized in that: described external preparation is patch, dressing, membrane, adhesive.
9. pharmaceutical composition according to claim 8, is characterized in that: described patch is hydrogel patch, foamed materials patch; Described adhesive is pressure sensitive adhesive.
10. the purposes of pharmaceutical composition described in claim 6 ~ 9 any one in the medicine of preparation hemostasis, wound healing.
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CN109689064A (en) * 2016-07-12 2019-04-26 埃尔科比奥有限公司 Wound healing and bone regeneration composition containing Uldavioside A compound
CN111118734A (en) * 2020-01-16 2020-05-08 东莞华工创为生物科技有限公司 Polyvinyl alcohol/carboxymethyl chitosan nanofiber medical dressing and preparation method and application thereof

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CN109689064A (en) * 2016-07-12 2019-04-26 埃尔科比奥有限公司 Wound healing and bone regeneration composition containing Uldavioside A compound
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CN111118734B (en) * 2020-01-16 2021-06-01 东莞华工创为生物科技有限公司 Polyvinyl alcohol/carboxymethyl chitosan nanofiber medical dressing and preparation method and application thereof

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