CN104288194B - A kind of preparation method of compound isatis root granules - Google Patents
A kind of preparation method of compound isatis root granules Download PDFInfo
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- CN104288194B CN104288194B CN201410596822.0A CN201410596822A CN104288194B CN 104288194 B CN104288194 B CN 104288194B CN 201410596822 A CN201410596822 A CN 201410596822A CN 104288194 B CN104288194 B CN 104288194B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 241000334160 Isatis Species 0.000 title claims abstract description 12
- 150000001875 compounds Chemical class 0.000 title claims abstract description 12
- 239000008187 granular material Substances 0.000 title claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 72
- 239000010231 banlangen Substances 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 15
- 102000004190 Enzymes Human genes 0.000 claims abstract description 15
- 229940088598 enzyme Drugs 0.000 claims abstract description 15
- 239000000706 filtrate Substances 0.000 claims abstract description 15
- 239000002245 particle Substances 0.000 claims abstract description 13
- 108010059892 Cellulase Proteins 0.000 claims abstract description 12
- 108010029182 Pectin lyase Proteins 0.000 claims abstract description 12
- 229940106157 cellulase Drugs 0.000 claims abstract description 12
- 239000004375 Dextrin Substances 0.000 claims abstract description 11
- 229920001353 Dextrin Polymers 0.000 claims abstract description 11
- 235000019425 dextrin Nutrition 0.000 claims abstract description 11
- 229930006000 Sucrose Natural products 0.000 claims abstract description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 10
- 239000000284 extract Substances 0.000 claims abstract description 10
- 239000005720 sucrose Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 239000006228 supernatant Substances 0.000 claims abstract description 9
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 238000001556 precipitation Methods 0.000 abstract description 15
- 238000000034 method Methods 0.000 abstract description 13
- 230000008569 process Effects 0.000 abstract description 9
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- 239000004382 Amylase Substances 0.000 description 4
- 102000013142 Amylases Human genes 0.000 description 4
- 108010065511 Amylases Proteins 0.000 description 4
- COHYTHOBJLSHDF-UHFFFAOYSA-N Indigo Chemical compound N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
- 235000019418 amylase Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000002386 leaching Methods 0.000 description 4
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- 239000008107 starch Substances 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
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- 239000002126 C01EB10 - Adenosine Substances 0.000 description 2
- 241001062009 Indigofera Species 0.000 description 2
- 235000000177 Indigofera tinctoria Nutrition 0.000 description 2
- 241000334154 Isatis tinctoria Species 0.000 description 2
- 208000005647 Mumps Diseases 0.000 description 2
- 206010000269 abscess Diseases 0.000 description 2
- 229960005305 adenosine Drugs 0.000 description 2
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- 201000007100 Pharyngitis Diseases 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 206010039587 Scarlet Fever Diseases 0.000 description 1
- 241000238370 Sepia Species 0.000 description 1
- 241000257673 Strobilanthes cusia Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 201000001028 acute contagious conjunctivitis Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 206010001093 acute tonsillitis Diseases 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- -1 alkaloids class Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
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- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000005321 cobalt glass Substances 0.000 description 1
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- 239000002552 dosage form Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 210000000867 larynx Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
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- 238000000465 moulding Methods 0.000 description 1
- 231100000862 numbness Toxicity 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
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- 230000003612 virological effect Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Animal Behavior & Ethology (AREA)
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- Alternative & Traditional Medicine (AREA)
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- Botany (AREA)
- Biotechnology (AREA)
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Abstract
The invention discloses a kind of preparation method of compound isatis root granules, comprise the following steps：By Radix Isatidis raw material, secondary, 2 hours for the first time, second 1 hour, collecting decoction, filtration are added water to cook；2) it is 1.10 that filtrate, which is concentrated into relative density, and temperature 45 C adds pectase, cellulase, the complex enzyme of the composition of pectin lyase and digested, pectase, cellulase, the weight ratio of pectin lyase are 5:3:2, complex enzyme addition is 0.5 1.0% of filtrate weight after concentrating；It is 1.20 to continue to be concentrated into relative density, and temperature 45 C is filtered, cooling, and adding 95% ethanol makes alcohol content be 30%, and quiescent setting extracts supernatant；Ethanol is reclaimed, it is 1.20 that relative density is concentrated into when temperature is 70 DEG C；The sucrose and dextrin of conventional amount used are added, particle is made, is dried.The present invention is by adding enzymolysis process, it is possible to reduce concentration of alcohol during alcohol precipitation, reduces cost.
Description
Technical field
The present invention relates to a kind of preparation method of medicine, and in particular to a kind of preparation method of compound isatis root granules.
Background technology
Radix Isatidis (conventional alias：Indigo-blue, indigo root, manyflower glorybower root) it is a kind of Chinese medicine.Produce all over China.Radix Isatidis
Be divided into northern Radix Isatidis and rhizoma et Radix Baphicacanthis Cusiae, northern Radix Isatidis source for cruciferae isatis (Isatis tinctoria L.) and
The root of careless smalt (I.indigotica Fort.)；Rhizoma et Radix Baphicacanthis Cusiae is acanthaceous vegetable acanthaceous indigo (Baphicacanthus cusia
(Nees) Brem.) rhizome and root, the effect of with clearing heat and detoxicating, preventing cold, relieving sore-throat.Cure mainly：Febrile virulent maculae, hyperpyrexia head
Bitterly, major part pestilence, tongue dark reddish purple dark, scarlet fever, erysipelas, mumps, larynx numbness, sore swells, carbuncle swells, varicella, measles, hepatitis, popularity
Flu, epidemic meningitis, encephalitis, pneumonia, coma hematemesis, pharynx swelling, pinkeye, sore rash；Can prevent and treat Japanese Type-B encephalitis, acute, chronic hepatitis,
Mumps, osteomyelitis.
Compound isatis root granules, main component is Radix Isatidis, and auxiliary material is sucrose, and dextrin is the particle of brown or sepia；
Taste sweet tea, slight bitter.Mainly there are two kinds of sugar-containing type and Sugarless type.Compound isatis root granules, clearing heat and detoxicating, cool blood, for anemopyretic cold,
Abscess of throat.Radix Isatidis is taken during flu, is conducive to enhancing immune, virus and pathogenic bacteria in vivo is killed, is conducive to rehabilitation；Stream
During sense and Radix Isatidis is taken during viral disease, is conducive to building up resistance, it is to avoid is infected.For caused by lung and stomach excessive heat
Abscess of throat, pharyngoxerosis, acute tonsillitis.
At present, the preparation method of compound isatis root granules is：By Radix Isatidis 1400g, add water to cook it is secondary, it is 2 small for the first time
When, second 1 hour, collecting decoction, filtration, filtrate was concentrated into relative density for 1.20 (50 DEG C), plus ethanol makes the alcohol content be
60%, standing makes precipitation, takes supernatant, reclaims ethanol and is concentrated into appropriate.Thick paste is taken, appropriate sucrose and dextrin, system is added
Into particle, dry, 1000g is made；Or thick paste is taken, appropriate dextrin and sweetener is added, particle is made, dries, 600g is made
(Sugarless type), is produced.Such preparation method comes with some shortcomings, and drops to room temperature as decoction must be placed to temperature and could add
Ethanol, but due to the gelatinization of starch, decoction is changed into g., jelly-like, is difficult now to enter ethanol to be dissolved with inside g., jelly-like thing
Composition is imitated, while being deposited the gelatinized starch got off has wrapped up active ingredient, there is that ethanol consumption is big, active ingredient is easily wrapped
Precipitation causes the shortcoming of yield reduction.
In summary, the optimization to the preparation method of existing compound isatis root granules is necessary.
The content of the invention
The technical problem to be solved in the present invention is in view of the shortcomings of the prior art, to the preparation method of compound isatis root granules
Optimize, improve production efficiency, reduce cost.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
1) by Radix Isatidis raw material, secondary, 2 hours for the first time, second 1 hour, collecting decoction, filtration are added water to cook；
2) filtrate is concentrated into relative density for 1.10, temperature 45 C, adds pectase, cellulase, pectin lyase
The complex enzyme of composition is digested, and pectase, cellulase, the weight ratio of pectin lyase are 5:3:2, complex enzyme addition is
The 0.5-1.0% of filtrate weight after concentration；
3) it is 1.20 to continue to be concentrated into relative density, and temperature 45 C is filtered, cooling, and adding 95% ethanol makes the alcohol content be
30%, quiescent setting extracts supernatant；
4) ethanol is reclaimed, it is 1.20 that relative density is concentrated into when temperature is 70 DEG C；
5) sucrose and dextrin of conventional amount used are added, particle is made, is dried.
It is of the present invention to decoct, using prior art common process.
Compared with prior art, the present invention has advantages below：
1st, water extraction and alcohol precipitation method is the Hydrolysis kinetics method commonly used during Chinese medicine preparation is produced.Concentration of alcohol is solid to Chinese medicine during alcohol precipitation
Body preparation curative effect, taking dose, dosage form selection, preparations shaping, product stability etc. have a major impact, second during reasonable selection alcohol precipitation
Determining alcohol is one of key link during solid preparation of traditional Chinese medicine.However, at present in the research of water extraction and alcohol precipitation method alcohol precipitation process,
General main examination alcohol precipitation process influence factor, such as relative density of traditional Chinese medicine extraction concentrated liquor, alcohol precipitation temperature, the alcohol precipitation time and
Plus alcohol mode etc. is to traditional Chinese medicinal ointment index components content, the influence of yield of extract, seldom research alcohol precipitation when ethanol consumption to whole system
The influence of agent process etc., often brings problem to follow-up production process and preparation stability.The present invention by adding enzymolysis process,
Concentration of alcohol during alcohol precipitation can be reduced, cost is reduced.
2nd, amylase is added when prior art has research using leaching process, but because starch is from the leaching of medicinal material organization internal
Profit-dissolving-diffusion process is far longer than the inactivation speed of amylase, causes effect to control.Somebody's research uses 80 DEG C
Soak extraction Radix Isatidis extractum, although greatly reduce the leaching of starch, but also greatly reduce the leaching of active ingredient simultaneously,
Cause the waste of herb resource.Carbohydrate (polysaccharide, oligosaccharides), adenosine, organic acid, amino acids, life are mainly contained in Radix Isatidis
The compounds such as alkaloids class, and the present invention, compared to single enzyme, is targetedly worked by the application to complex enzyme, can be compared with
Decompose polysaccharose substance well, it is to avoid above drawback, effect is obvious.
3rd, by the process optimization of the present invention, to the Radix Isatidis index components adenosine rate of transform, yield of extract, and to spraying
Drying process, medicinal extract dry jet mixing pile, moulding process all bring larger improvement.
Embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1：
1) by Radix Isatidis raw material, secondary, 2 hours for the first time, second 1 hour, collecting decoction, filtration are added water to cook；
2) filtrate is concentrated into relative density for 1.10, temperature 45 C, adds pectase, cellulase, pectin lyase
The complex enzyme of composition is digested, and pectase, cellulase, the weight ratio of pectin lyase are 5:3:2, complex enzyme addition is
The 0.5% of filtrate weight after concentration；
3) it is 1.20 to continue to be concentrated into relative density, and temperature 45 C is filtered, cooling, and adding 95% ethanol makes the alcohol content be
30%, quiescent setting extracts supernatant；
4) ethanol is reclaimed, it is 1.20 that relative density is concentrated into when temperature is 70 DEG C；
5) sucrose and dextrin of conventional amount used are added, particle is made, is dried.
Embodiment 2：
1) by Radix Isatidis raw material, secondary, 2 hours for the first time, second 1 hour, collecting decoction, filtration are added water to cook；
2) filtrate is concentrated into relative density for 1.10, temperature 45 C, adds pectase, cellulase, pectin lyase
The complex enzyme of composition is digested, and pectase, cellulase, the weight ratio of pectin lyase are 5:3:2, complex enzyme addition is
The 1.0% of filtrate weight after concentration；
3) it is 1.20 to continue to be concentrated into relative density, and temperature 45 C is filtered, cooling, and adding 95% ethanol makes the alcohol content be
30%, quiescent setting extracts supernatant；
4) ethanol is reclaimed, it is 1.20 that relative density is concentrated into when temperature is 70 DEG C；
5) sucrose and dextrin of conventional amount used are added, particle is made, is dried.
Embodiment 3：
1) by Radix Isatidis raw material, secondary, 2 hours for the first time, second 1 hour, collecting decoction, filtration are added water to cook；
2) filtrate is concentrated into relative density for 1.10, temperature 45 C, adds pectase, cellulase, pectin lyase
The complex enzyme of composition is digested, and pectase, cellulase, the weight ratio of pectin lyase are 5:3:2, complex enzyme addition is
The 0.8% of filtrate weight after concentration；
3) it is 1.20 to continue to be concentrated into relative density, and temperature 45 C is filtered, cooling, and adding 95% ethanol makes the alcohol content be
30%, quiescent setting extracts supernatant；
4) ethanol is reclaimed, it is 1.20 that relative density is concentrated into when temperature is 70 DEG C；
5) sucrose and dextrin of conventional amount used are added, particle is made, is dried.
Comparative example 1：
By Radix Isatidis medicine materical crude slice, secondary, 2 hours for the first time, second 1 hour, collecting decoction, filtration, filtrate are added water to cook
Relative density is concentrated into for 1.20 (50 DEG C), plus ethanol makes alcohol content be 60%, standing makes precipitation, takes supernatant, reclaims ethanol
And be concentrated into appropriate.Thick paste is taken, appropriate sucrose and dextrin is added, particle is made, is dried.
Comparative example 2：
1) by Radix Isatidis raw material, secondary, 2 hours for the first time, second 1 hour, collecting decoction, filtration are added water to cook；
2) it is 1.10 that filtrate, which is concentrated into relative density, temperature 45 C, adds amylase, and amylase addition is filter after concentration
The 0.8% of liquid weight；
3) it is 1.20 to continue to be concentrated into relative density, and temperature 45 C is filtered, cooling, and adding 95% ethanol makes the alcohol content be
50%, quiescent setting extracts supernatant；
4) ethanol is reclaimed, it is 1.20 that relative density is concentrated into when temperature is 70 DEG C；
5) sucrose and dextrin of conventional amount used are added, particle is made, is dried.
Table one：Embodiment and comparative example data statistics
Conclusion：The present invention in Aqueous extracts concentration process by adding complex enzyme, ethanol when can significantly reduce alcohol precipitation
Consumption, to the compound isatis root granules obtained by subsequent technique, particle yield, particle friability, the long altar on which incense burners are placed rate of transform with
And the spray drying index such as water content all has clear improvement, cost is reduced while quality is improved.
Claims (1)
1. a kind of preparation method of compound isatis root granules, it is characterised in that comprise the following steps：
1) by Radix Isatidis raw material, secondary, 2 hours for the first time, second 1 hour, collecting decoction, filtration are added water to cook；
2) it is 1.10 that filtrate, which is concentrated into relative density, and temperature 45 C adds pectase, cellulase, the composition of pectin lyase
Complex enzyme digested, pectase, cellulase, the weight ratio of pectin lyase are 5:3:2, complex enzyme addition is concentration
The 0.5-1.0% of filtrate weight afterwards；
3) it is 1.20 to continue to be concentrated into relative density, and temperature 45 C is filtered, cooling, and adding 95% ethanol makes alcohol content be 30%,
Quiescent setting, extracts supernatant；
4) ethanol is reclaimed, it is 1.20 that relative density is concentrated into when temperature is 70 DEG C；
5) sucrose and dextrin of conventional amount used are added, particle is made, is dried.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410596822.0A CN104288194B (en) | 2014-10-30 | 2014-10-30 | A kind of preparation method of compound isatis root granules |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410596822.0A CN104288194B (en) | 2014-10-30 | 2014-10-30 | A kind of preparation method of compound isatis root granules |
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| Publication Number | Publication Date |
|---|---|
| CN104288194A CN104288194A (en) | 2015-01-21 |
| CN104288194B true CN104288194B (en) | 2017-08-08 |
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| CN105432899A (en) * | 2015-12-31 | 2016-03-30 | 桂林双象生物科技有限公司 | Preparation method of honeysuckle flower and grosvenor momordica fruit composite tea |
| CN105477347A (en) * | 2015-12-31 | 2016-04-13 | 吉林紫鑫药业股份有限公司 | Method for preparing medicine used for treating liver-stomach disharmony and blood stasis collateral obstruction |
| CN106692490A (en) * | 2016-12-13 | 2017-05-24 | 广西双蚁药业有限公司 | Sucrose-free compound isatis root granules and preparation method thereof |
| CN107349247A (en) * | 2017-05-23 | 2017-11-17 | 回音必集团安徽制药有限公司 | A kind of Radix Isatidis Optimized Extraction Process |
| CN107582600A (en) * | 2017-06-26 | 2018-01-16 | 安徽永生堂药业有限责任公司 | A kind of banlangen granules, banlangen keli and preparation method thereof |
| CN107951910A (en) * | 2017-12-25 | 2018-04-24 | 安徽永生堂药业有限责任公司 | A kind of preparation method of compound isatis root granules electuary |
| CN108969604A (en) * | 2018-08-15 | 2018-12-11 | 康美保宁（四川）制药有限公司 | A kind of pre-treating method and preparation method of compound isatis root granules |
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Denomination of invention: A kind of preparation method of compound isatidis granules Effective date of registration: 20220921 Granted publication date: 20170808 Pledgee: Bohai Bank Co.,Ltd. Zhongshan branch Pledgor: GUANGDONG XIANGSHANTANG PHARMACEUTICAL CO.,LTD. Registration number: Y2022980015849 |