CN104287032B - Ulva juice product containing ulva oligosaccharide and preparation method thereof - Google Patents

Ulva juice product containing ulva oligosaccharide and preparation method thereof Download PDF

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Publication number
CN104287032B
CN104287032B CN201410462306.9A CN201410462306A CN104287032B CN 104287032 B CN104287032 B CN 104287032B CN 201410462306 A CN201410462306 A CN 201410462306A CN 104287032 B CN104287032 B CN 104287032B
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ulva
oligosaccharide
juice
polysaccharide
goods
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CN104287032A (en
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江晓路
李国霞
沈照鹏
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Ocean University of China
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Ocean University of China
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives

Abstract

The present invention is a series of ulva juice product containing oligosaccharide and preparation method thereof, the ulva polysaccharide utilizing polysaccharide sulfate digestive enzyme can not be digested and assimilated by human body is degraded into specific ulva oligosaccharide, dispersion frond cell, increase cell permeability, with efficiently extracting ulva effective ingredient, thus obtain full nutrition ulva juice, reduce ulva polysaccharide stickiness and gelling, improve mouthfeel and the nutrition of ulva goods.In gained ulva juice oligosaccharide molecular weight distribution < 5000Da, content more than 25 mg/g(in terms of ulva dry weight).Ulva juice is again through PROCESS FOR TREATMENT, it is thus achieved that ulva soluble preparation.These goods can directly develop into health promoting beverage, flavoring agent etc., can act also as raw material for industries such as food, cosmetics, health product, medicines.This invention makes full use of abundant ulva resource, develops the daily edible ulva goods of applicable people, is convenient for people to absorb the nutrient substance in ulva, improves the quality of life of people.

Description

Ulva juice product containing ulva oligosaccharide and preparation method thereof
Technical field
The present invention relates to biotechnology and field of food, specifically refer to the ulva juice product containing ulva oligosaccharide and preparation side thereof Method.
Background technology
Ulva is also known as sea lettuce " Chinese medicinal herbal ", Ulva lactuca L., Ulva lactuca (" Chinese Plants illustrated handbook "), green dish, moss dish etc., is Belong to the large-scale chlorella of Chlorophyta Shi Yao section Ulva.This genus has more than 30 to plant, and China there are about 6 kinds, and frequent species has U. pertusa (Uiva pertusa), dish ulva (Uiva lactuca) and sliver ulva (Uiva fasciata) etc., be distributed in Liaoning, river North, Shandong, Jiangsu, Zhejiang are coastal to the ground such as Guangdong, Hainan Island, the most rare by north orientation south.The tender ulva of children is well to eat Product, can be used as medicine, it is possible to be used for making refreshment drink, and ripe frond is the quality raw materials of allotment livestock or poultry mixed feed.Stone The nutritive value of Herba braseniaeel. is the highest, and its dry product is containing protein 16.03%, fat 0.23%, crude fibre 6.89%, pentosan 12.27%, except this Outside, possibly together with unsaturated fatty acid, fatsoluble vitamin, vitamin B group, vitamin C and mineral such as abundant linolenic acid Se content in the nutritional labelings such as matter, particularly dry product ulva is 5.980 ug/g, hence it is evident that higher than in 11 kinds of economic algaes such as Thallus Laminariae (Thallus Eckloniae) Se content.Containing 12 kinds of aminoacid in ulva crude protein, wherein there are 8 kinds for essential amino acid.Recent studies shows, stone Herba braseniaeel. has the merits such as blood fat reducing, anticoagulation, purification blood, antiviral, cholesterol reducing, antitumor and adjuvant therapy of cardiovascular disease Effect.Its main active is polysaccharide, monosaccharide A wide selection of colours and designs contained by ulva polysaccharide, have rhamnose, glucose, xylose, triose, Fucose, mannose, galactose, 8 kinds of monosaccharide of arabinose, but predominantly rhamnose, glucose, xylose, belong to " glucal Acid-xylose-rhamnose polymer-type " polysaccharide, wherein rhamnose content is the highest, and it is different to produce region, rhamnose and glucose Ratio the most different, compared with the edible green algae polysaccharide of other kinds, ulva polysaccharide sulfate content is the highest.But, for a long time Since ulva application in terms of food mainly with by conventional culinary, the simple processing method such as bake and make with primary Product is main, has the shortcomings such as material nutrient component utilization rate is low, added value is the highest, the market competitiveness is poor.
Present invention problem appeared in China's marine algae resource exploitation is started with, and has invented a set of with ulva as raw material Enzymolysis ulva prepares ulva natural juice, condensed cream and the method for instant full matter dry powder.Ulva juice is instant natural drink, can make clear Cool beverage, it remains in ulva overwhelming majority nutritional labelings, and appearance uniform is suspended, in bright green, containing ulva oligosaccharide, amino The functional factors such as acid, vitamin and mineral element, can be as the raw material of exploitation health food.Ulva as main component is few Sugar, it is easy to body absorbs, has immunomodulating, purifies blood, maintenance blood vessel, blood fat reducing, antibacterial, activating cell, antioxidation, anti- The multiple physiologically actives such as aging.The ulva of different cultivars all can be processed into the ulva juice rich in activated oligosaccharide, can be widely applied to Food service industry, can be developed into the high value added product such as pharmaceutical products, health product after deep processing.Present invention exploitation and enforcement utilize Toolenzyme carries out high-valued processing and the production of full nutrition ulva, the nutrient substance of development and utilization ulva efficiently.This invention Give full play to ulva aboundresources, trophic factors is complete, health value is high advantage, invent the health care being suitably daily drunk by people Beverage, enriches nutrition and the taste of beverage, improves the quality of life of people.
Summary of the invention
The present invention relates to prepare the ulva juice product containing oligosaccharide with enzymatic isolation method, ulva refers to dish ulva (Uiva Lactuca), U. pertusa (Uiva pertusa) and sliver ulva (Uiva fasciata) etc., ulva enzymolysis goods include former Juice, condensed cream, solubility full nutrition dry powder and preparation method thereof.The technical scheme is that
Ulva natural juice and preparation method: the ulva of fresh collection rinses silt, foreign material repeatedly through flowing water, dries, and is crushed to Powder (40 mesh) is standby.Taking a certain amount of ulva lactuca powder, add water with solid-liquid ratio 1:10～50 and extract 1 h in 100 DEG C, cooling, in 30 After DEG C insulation 30 min, add the polysaccharide sulfate digestive enzyme 1000U/g of ulva dry weight (addition of enzyme be), stir evenly, and put Enter enzymolysis 4～8 h in 30 DEG C of calorstats, course of reaction is stirred continuously, so that enzyme digestion reaction is fully carried out.After reaction terminates, Boil enzymolysis reaction.Enzymolysis ulva liquid is centrifugal 10 min under 4000 rpm rotating speeds, take supernatant, are prepared by enzymolysis Ulva natural juice.Survey its content of reducing sugar and can prepare ulva oligosaccharide amount more than 25 mg more than 0.5 mg/mL, every gram of ulva dry powder.
The mensuration of ulva oligosaccharide molecular amount distribution: take a certain amount of above-mentioned ulva natural juice, add 1 times of volume dehydrated alcohol, Under 4 C, stand 2 h, be centrifuged off the impurity such as albumen, then with the dehydrated alcohol of 5 times, the oligosaccharide in ulva juice precipitated, centrifugal Removing supernatant, dissolve with distilled water, centrifugal going is precipitated, and supernatant electron spray ionization mass spectrometer measures the molecule of oligosaccharide Amount.Electrospray ionization mass spectrometry (Electrospray-ionization mass spectroscopy, ESI-MS) use cloudy from Subpattern.Sample is dissolved in MeOH-H2(1:1) (10 pmol mL in O solution-1) and with syringe pump with 5 L min-1Speed Inject EFI source.Mass scan range is from 120 to 2000 m/z.Capillary temperature is maintained at 250 C, nitrogen for atomization and Desolvation gas.Molecular weight distribution < 2500Da, the degree of polymerization 4～10 of measured ulva oligosaccharide.Shown in measurement result Fig. 1.
By the ulva natural juice containing oligosaccharide of above-mentioned preparation, it is evaporated under reduced pressure under 40～80 C and concentrates 10～20 times, prepare Ulva juice condensed cream containing oligosaccharide.
The ulva natural juice containing oligosaccharide of above-mentioned preparation (or is added a certain amount of amylodextrin, to improve ulva in advance The response rate of juice dry powder, prevents dry powder caking from improving storge quality), it is spray-dried under conditions of air intake 160 C, air-out 90 C Prepare the ulva juice dry powder containing oligosaccharide.
Beneficial effects of the present invention: the present invention is based on dish ulva, U. pertusa and sliver ulva, and to have identical ulva many Sugar, this polysaccharide is the prepared ulva juice product containing ulva oligosaccharide and system thereof after the degraded of polysaccharide sulfate digestive enzyme special process Preparation Method, carries out the high-valued processing of full nutrition ulva.The feature of this technology is to be degraded into specific by the ulva polysaccharide in ulva Ulva oligosaccharide, disperse frond cell, increase cell permeability, with efficiently extracting ulva effective ingredient, reduce ulva many simultaneously Sugar stickiness and gelling, the ulva polysaccharide that human body is difficult to digest and assimilate is degraded to the specific activated oligosaccharide fragment easily absorbed, Improve mouthfeel and the nutrition of ulva goods.Again ulva juice is carried out PROCESS FOR TREATMENT, it is thus achieved that the ulva containing specific oligosaccharide is solvable Property goods, these goods be modulated can directly develop into health promoting beverage, flavoring agent etc., it is possible to be processed further as raw material Develop into the functional article such as food, cosmetics, health product, medicine.This invention gives full play to ulva aboundresources, trophic factors Entirely, advantage that health value is high, develop the daily edible ulva goods of applicable people, be convenient for people to absorb the nutrition in ulva Material, improves the quality of life of people.
Accompanying drawing illustrates:
Fig. 1 is the range of molecular weight distributions mass spectrum of ulva oligosaccharide in product.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described
Embodiment 1
Taking ulva lactuca powder 100 g, add water 5000 mL, is heated to 100 DEG C, and insulated and stirred 1 h is cooled to room temperature, in 30 DEG C of guarantors After temperature 30 min, add polysaccharide sulfate digestive enzyme 1 g, stir evenly, and put into enzymolysis 6.5 h in 30 DEG C of calorstats.Course of reaction In be stirred continuously, make enzyme digestion reaction fully carry out.After reacting 6.5 h, boil enzymolysis reaction.Enzymolysis ulva liquid is 4000 Under rpm rotating speed, centrifugal 10 min, take supernatant, are ulva natural juice prepared by enzymolysis, survey its content of reducing sugar more than 0.583 Mg/mL, i.e. every gram ulva dry powder can prepare ulva oligosaccharide amount more than 27.984 mg.By the ulva natural juice average mark of above-mentioned preparation Becoming two parts, a reduction vaporization under 55 C concentrates about 20 times, prepares ulva juice condensed cream 120 mL containing oligosaccharide；Another Part is spray-dried under conditions of air intake 160 C, air-out 90 C and prepares the ulva juice dry powder 31.5g containing oligosaccharide.
Embodiment 2
Taking ulva lactuca powder 100 g, add water 2000 mL, is heated to 100 DEG C, and insulated and stirred 1 h is cooled to room temperature, in 30 DEG C of guarantors After temperature 30 min, add polysaccharide sulfate digestive enzyme (being purchased from sigma company) 1 g, stir evenly, and put into enzymolysis in 30 DEG C of calorstats 6.5 h.Course of reaction is stirred continuously, makes enzyme digestion reaction fully carry out.After reacting 6.5 h, boil enzymolysis reaction.Enzymolysis Ulva liquid is centrifugal 10 min under 4000 rpm rotating speeds, take supernatant, are ulva natural juice prepared by enzymolysis.Survey its reducing sugar to contain Amount can prepare ulva oligosaccharide amount more than 25 mg more than 1.3mg/mL, i.e. every gram ulva dry powder.The ulva natural juice of above-mentioned preparation is put down Being divided into two parts, a reduction vaporization under 55 C concentrates about 12 times, prepares ulva juice condensed cream 75 mL containing oligosaccharide； Another part is spray-dried under conditions of air intake 160 C, air-out 90 C and prepares the ulva juice dry powder 30.55g containing oligosaccharide.

Claims (2)

1. the ulva juice prepared with ulva, is characterized in that enzymic degradation afterproduct contains ulva oligosaccharide, ulva oligosaccharide molecular Amount distribution < 5000Da, degree of polymerization 4-10, containing sulfate group, its content in terms of ulva raw material dry weight more than 25 mg/g.
The most according to claim 1, the ulva juice prepared with ulva, is characterized in that ulva used is dish ulva Uiva Lactuca, U. pertusa Uiva pertusa and sliver ulva Uiva fasciata.
CN201410462306.9A 2014-09-12 2014-09-12 Ulva juice product containing ulva oligosaccharide and preparation method thereof Active CN104287032B (en)

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3057770B1 (en) * 2016-10-21 2019-07-19 Seprosys USE OF A ULVE EXTRACT AS ANTI-AGE COSMETIC AGENT

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005102639A (en) * 2003-10-01 2005-04-21 Marutomo Co Ltd Constipation improving food and method for producing the same
CN103054155A (en) * 2013-01-31 2013-04-24 中国烟草总公司郑州烟草研究院 Application of ulva water extract in cigarette
CN103589569A (en) * 2013-11-26 2014-02-19 青岛嘉瑞生物技术有限公司 Production technique of compound fermented ulva health wine
CN103584256A (en) * 2013-11-26 2014-02-19 青岛嘉瑞生物技术有限公司 Natural food preservative prepared by utilizing ulva resource development and preparation method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005102639A (en) * 2003-10-01 2005-04-21 Marutomo Co Ltd Constipation improving food and method for producing the same
CN103054155A (en) * 2013-01-31 2013-04-24 中国烟草总公司郑州烟草研究院 Application of ulva water extract in cigarette
CN103589569A (en) * 2013-11-26 2014-02-19 青岛嘉瑞生物技术有限公司 Production technique of compound fermented ulva health wine
CN103584256A (en) * 2013-11-26 2014-02-19 青岛嘉瑞生物技术有限公司 Natural food preservative prepared by utilizing ulva resource development and preparation method

Non-Patent Citations (1)

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Title
含褐藻寡糖调味汁的研制;沈照鹏;《食品工业科技》;20120730;第33卷(第7期);第305页左栏前言，30-7-308页3.结论 *

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