CN104255522A - Rapid propagation method for plant regeneration of pyrrosia sheareri (Bak.) Ching - Google Patents
Rapid propagation method for plant regeneration of pyrrosia sheareri (Bak.) Ching Download PDFInfo
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- CN104255522A CN104255522A CN201410540654.3A CN201410540654A CN104255522A CN 104255522 A CN104255522 A CN 104255522A CN 201410540654 A CN201410540654 A CN 201410540654A CN 104255522 A CN104255522 A CN 104255522A
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Abstract
The invention relates to a rapid propagation method for plant regeneration of pyrrosia sheareri (Bak.) Ching. The method comprises disinfection of leaves, induction of callus tissue, mutagenesis of callus tissue, differentiation of callus tissue and rooting culture. With adoption of the method, the regeneration rate is high, the hereditary stability is good, obtaining of new strain of pyrrosia sheareri (Bak.) Ching is facilitated, and new mutant germplasm resources or varieties are selected.
Description
Technical field
The present invention relates to the quick-breeding method of pyrrosia sheareriChing tissue cultures, belong to plant technology field.
Background technology
Pyrrosia sheareriChing,
pyrrosia sheareri(Bak.) Ching, Polypodiaceae, another name: henry colysis herb, tabula rasa pyrrosia lingua.Perennial herb, high 30-60cm.Rhizome is sturdy, horizontal walk, close raw lanceolar scale, scale edge ciliation.Leaf homotype, clusters, hard keratin, above only along vein hairiness or without hair, has fine and closely woven and irregular concave point, has bifurcated, short wealthy yellow stellate hair below; Petiole is sturdy, be born on rhizome with joint; The wide lanceolar of blade, long 20-40cm, wide 3-5cm, gradually sharp to top, slightly broadening to base portion, is the round ear shape of not waiting; Lateral vein is slightly recessed on two sides.A sporangium group of mean people, is arranged in multirow between lateral vein, uncovered.Be distributed in each provinces and regions on the south the Changjiang river.On raw stone or on trunk, height above sea level 500-2290 rice.Herb, can hyoscine containing the chemical composition such as different mangiferin, fumaric acid, caffeic acid, saponin, anthracene glucoside, glycosides displayed, tannin, function with cure mainly: promoting diuresis for stranguria, cooling blood and hemostasis.For scanty drak urine, drench the puckery pain of drop, blood drenches, cough.Gather in the main field that adopts at present, and the research of tissue cultures not yet starts.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of pyrrosia sheareriChing plant regeneration, and adopt the inventive method regeneration rate high, genetic stability is good, contributes to obtaining pyrrosia sheareriChing new lines, the mutant germ plasm resource that seed selection makes new advances or kind.
Technical problem to be solved by this invention is realized by following scheme:
Get pyrrosia sheareriChing young leaflet tablet, 5min is soaked in bleaching powder, running water 20min, 1% bromine water process 9min on superclean bench, aseptic water washing 5 times, the pyrrosia sheareriChing blade access medium of disinfecting is KC+6-BA2.0mg/L+2, callus induction is carried out in 4-D2.0mg/L, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 600lx, photophase 12h/d, temperature 24 ± 1 DEG C, induce the callus Ultraviolet radiation 30s after a week, lithium chloride process 10min, carry out differentiation in the callus access KC+6-BA2.5-3.0mg/L+IBA0.3-0.5mg/L medium that mutagenic treatment is crossed to cultivate, additional saccharose 30g/L, agar 6.5g/L, PH6.0, illumination 6000lx, photophase 12h/d, temperature 25 DEG C, root induction is carried out in bud seedling access KC+IBA0.02 medium after differentiation, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 16000lx, photophase 18h/d, temperature 25 DEG C.
The pyrrosia sheareriChing regeneration motility rate adopting the present invention to prepare is high, and the cycle is short, and output is large, pollutes little, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Get pyrrosia sheareriChing young leaflet tablet, 5min is soaked in bleaching powder, running water 20min, 1% bromine water process 9min on superclean bench, aseptic water washing 5 times, the pyrrosia sheareriChing blade access medium of disinfecting is KC+6-BA2.0mg/L+2, callus induction is carried out in 4-D2.0mg/L, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 600lx, photophase 12h/d, temperature 24 ± 1 DEG C, induce the callus Ultraviolet radiation 30s after a week, lithium chloride process 10min, carry out differentiation in the callus access KC+6-BA2.5mg/L+IBA0.3mg/L medium that mutagenic treatment is crossed to cultivate, additional saccharose 30g/L, agar 6.5g/L, PH6.0, illumination 6000lx, photophase 12h/d, temperature 25 DEG C, root induction is carried out in bud seedling access KC+IBA0.02 medium after differentiation, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 16000lx, photophase 18h/d, temperature 25 DEG C, regeneration motility rate 91%.
Embodiment 2
Get pyrrosia sheareriChing young leaflet tablet, 5min is soaked in bleaching powder, running water 20min, 1% bromine water process 9min on superclean bench, aseptic water washing 5 times, the pyrrosia sheareriChing blade access medium of disinfecting is KC+6-BA2.0mg/L+2, callus induction is carried out in 4-D2.0mg/L, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 600lx, photophase 12h/d, temperature 24 ± 1 DEG C, induce the callus Ultraviolet radiation 30s after a week, lithium chloride process 10min, carry out differentiation in the callus access KC+6-BA3.0mg/L+IBA0.5mg/L medium that mutagenic treatment is crossed to cultivate, additional saccharose 30g/L, agar 6.5g/L, PH6.0, illumination 6000lx, photophase 12h/d, temperature 25 DEG C, root induction is carried out in bud seedling access KC+IBA0.02 medium after differentiation, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 16000lx, photophase 18h/d, temperature 25 DEG C, regeneration motility rate 93%.
Embodiment 3
Get pyrrosia sheareriChing young leaflet tablet, 5min is soaked in bleaching powder, running water 20min, 1% bromine water process 9min on superclean bench, aseptic water washing 5 times, the pyrrosia sheareriChing blade access medium of disinfecting is KC+6-BA2.0mg/L+2, callus induction is carried out in 4-D2.0mg/L, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 600lx, photophase 12h/d, temperature 24 ± 1 DEG C, induce the callus Ultraviolet radiation 30s after a week, lithium chloride process 10min, carry out differentiation in the callus access KC+6-BA3.0mg/L+IBA0.4mg/L medium that mutagenic treatment is crossed to cultivate, additional saccharose 30g/L, agar 6.5g/L, PH6.0, illumination 6000lx, photophase 12h/d, temperature 25 DEG C, root induction is carried out in bud seedling access KC+IBA0.02 medium after differentiation, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 16000lx, photophase 18h/d, temperature 25 DEG C, regeneration motility rate 94%.
Claims (2)
1. a method for quickly breeding for pyrrosia sheareriChing plant regeneration, comprise the sterilization of blade, the induction of callus, the mutagenesis of callus, the differentiation of callus, culture of rootage, its key step is as follows:
(1) pyrrosia sheareriChing young leaflet tablet is got, to its disinfection;
(2) the pyrrosia sheareriChing blade access medium of step (1) being disinfected is carry out callus induction in KC+6-BA2.0mg/L+2,4-D2.0mg/L, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 600lx, photophase 12h/d, temperature 24 ± 1 DEG C;
(3) get step (2) and induce the callus Ultraviolet radiation 30s after a week, lithium chloride process 10min;
(4) get in the callus access KC+6-BA2.5-3.0mg/L+IBA0.3-0.5mg/L medium that step (3) mutagenic treatment crosses and carry out differentiation cultivation, additional saccharose 30g/L, agar 6.5g/L, PH6.0, illumination 6000lx, photophase 12h/d, temperature 25 DEG C;
(5) get in the bud seedling access KC+IBA0.02 medium after step (4) differentiation and carry out root induction, additional saccharose 30g/L, agar 6.5g/L, pH6.0, illumination 16000lx, photophase 18h/d, temperature 25 DEG C.
2. according to the method for quickly breeding of a kind of pyrrosia sheareriChing plant regeneration according to claim 1, it is characterized in that: the acquisition of the aseptic blade of pyrrosia sheareriChing described in step (1) is, get pyrrosia sheareriChing young leaflet tablet, 5min is soaked in bleaching powder, running water 20min, 1% bromine water process 9min on superclean bench, aseptic water washing 5 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410540654.3A CN104255522A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for plant regeneration of pyrrosia sheareri (Bak.) Ching |
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| CN201410540654.3A CN104255522A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for plant regeneration of pyrrosia sheareri (Bak.) Ching |
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| CN201410540654.3A Pending CN104255522A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for plant regeneration of pyrrosia sheareri (Bak.) Ching |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106699561A (en) * | 2017-01-13 | 2017-05-24 | 佳木斯大学 | Method for rapidly obtaining crude extract of chlorogenic acid |
| CN110024690A (en) * | 2019-04-03 | 2019-07-19 | 佳木斯大学 | The rapid propagation method of Usu Li Wawei |
| WO2022110864A1 (en) * | 2020-11-30 | 2022-06-02 | 中国热带农业科学院海口实验站 | Chemical mutagenesis method for increasing mutation range of dwarf bananas by means of induction |
-
2014
- 2014-10-14 CN CN201410540654.3A patent/CN104255522A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106699561A (en) * | 2017-01-13 | 2017-05-24 | 佳木斯大学 | Method for rapidly obtaining crude extract of chlorogenic acid |
| CN106699561B (en) * | 2017-01-13 | 2020-11-10 | 佳木斯大学 | Method for quickly obtaining chlorogenic acid crude extract |
| CN110024690A (en) * | 2019-04-03 | 2019-07-19 | 佳木斯大学 | The rapid propagation method of Usu Li Wawei |
| CN110024690B (en) * | 2019-04-03 | 2022-01-04 | 佳木斯大学 | Rapid propagation method of Wusuliwavi |
| WO2022110864A1 (en) * | 2020-11-30 | 2022-06-02 | 中国热带农业科学院海口实验站 | Chemical mutagenesis method for increasing mutation range of dwarf bananas by means of induction |
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Application publication date: 20150107 |
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| WD01 | Invention patent application deemed withdrawn after publication |