CN104255505A - Rapid propagation method for tissue culture of clematis armandii - Google Patents
Rapid propagation method for tissue culture of clematis armandii Download PDFInfo
- Publication number
- CN104255505A CN104255505A CN201410540415.8A CN201410540415A CN104255505A CN 104255505 A CN104255505 A CN 104255505A CN 201410540415 A CN201410540415 A CN 201410540415A CN 104255505 A CN104255505 A CN 104255505A
- Authority
- CN
- China
- Prior art keywords
- clematis herb
- temperature
- mandarin
- blade
- induction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention researches a rapid propagation method for tissue culture of clematis armandii. The method comprises steps as follows: obtaining of sterile leaves of the clematis armandii, induction of calluses, differentiation of the calluses, rooting culture and the like. The clematis armandii produced with the method is high in differentiation rate, short in growth cycle and low in production cost, and a theoretical research basis and technical support are provided for the development and the utilization of clematis armandii resources.
Description
Technical field
The present invention relates to the quick-breeding method of mandarin clematis herb tissue cultures, belong to plant technology field.
Background technology
Mandarin clematis herb,
clematis armandii, Ranunculaceae, also known as caulis clematidis armandii, clematis biondiana Pavolini, light akebi etc. are that one is evergreen climbs shape bejuco, Gao Keda 6 meters.Stem is cylindrical, tool vertical stripe, sprig tool rib, drape over one's shoulders white pubescence, after gradually drop to without hair, pitchy or micro-bronzing after dry, at distribution in China in Eastern Tibet, Yunnan, Guizhou, Sichuan, Gansu and Southern Shaanxi, Hubei, Hunan, Guangdong, Guangxi, the west and south, Fujian.Also there is distribution in Vietnam.This kind is used as medicine with rattan, and Chinese medicine is called caulis clematidis armandii, lightly seasoned, micro-hardship, cold in nature, slightly poisonous, the thoughts of returning home, small intestine and urinary bladder channel, and implantation methods Major Natural is bred.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of mandarin clematis herb, and the mandarin clematis herb differentiation rate prepared by the method is high, and growth cycle is short, and production cost is low, and the exploitation for mandarin clematis herb resource provide theoretical research foundation and technical basis.
Technical problem to be solved by this invention is realized by following scheme:
Get the tender blade of mandarin clematis herb children, running water 40min, on superclean bench 10% hydrogen peroxide treatment 8min, aseptic water washing 6 times to noresidue, the mandarin clematis herb blade access N disinfected
6carry out the induction of callus in+6-BA0.5mg/L+2,4-D0.5mg/L+Vc1g/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, half-light photograph, temperature 22 DEG C, the callus derived puts into medium N
6+ 6-BA0.5mg/L+KT0.7mg/L carries out induction cultivation, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, photophase 14h/d, temperature 24 DEG C, cultivate in the differentiation obtained bud seedling access MS+NAA0.1-0.15mg/L out, obtain healthy and strong bud seedling, additional saccharose 40g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 24 DEG C, the bud seedling being about 3cm takes out, be immersed in the Cu solion of 0.2% and soak 3min, 1min in the tray containing 10-30mg/LNAA solution is immersed in after taking-up, aseptic water washing is clean, insert loess: in the nutritious bag of rice husk=2:1, regular water spray, weeding, cover the shade net of 65%, temperature 28 DEG C, humidity 75%, and keep ventilated.
The mandarin clematis herb rooting rate adopting the present invention to prepare is high, and the cycle is short, and output is large, pollutes little, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Get the tender blade of mandarin clematis herb children, running water 40min, on superclean bench 10% hydrogen peroxide treatment 8min, aseptic water washing 6 times to noresidue, the mandarin clematis herb blade access N disinfected
6carry out the induction of callus in+6-BA0.5mg/L+2,4-D0.5mg/L+Vc1g/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, half-light photograph, temperature 22 DEG C, the callus derived puts into medium N
6+ 6-BA0.5mg/L+KT0.7mg/L carries out induction cultivation, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, photophase 14h/d, temperature 24 DEG C, cultivate in the differentiation obtained bud seedling access MS+NAA0.1mg/L out, obtain healthy and strong bud seedling, additional saccharose 40g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 24 DEG C, the bud seedling being about 3cm takes out, be immersed in the Cu solion of 0.2% and soak 3min, 1min in the tray containing 10mg/LNAA solution is immersed in after taking-up, aseptic water washing is clean, insert loess: in the nutritious bag of rice husk=2:1, regular water spray, weeding, cover the shade net of 65%, temperature 28 DEG C, humidity 75%, and keep ventilated, survival rate 89%.
Embodiment 2
Get the tender blade of mandarin clematis herb children, running water 40min, on superclean bench 10% hydrogen peroxide treatment 8min, aseptic water washing 6 times to noresidue, the mandarin clematis herb blade access N disinfected
6carry out the induction of callus in+6-BA0.5mg/L+2,4-D0.5mg/L+Vc1g/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, half-light photograph, temperature 22 DEG C, the callus derived puts into medium N
6+ 6-BA0.5mg/L+KT0.7mg/L carries out induction cultivation, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, photophase 14h/d, temperature 24 DEG C, cultivate in the differentiation obtained bud seedling access MS+NAA0.15mg/L out, obtain healthy and strong bud seedling, additional saccharose 40g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 24 DEG C, the bud seedling being about 3cm takes out, be immersed in the Cu solion of 0.2% and soak 3min, 1min in the tray containing 30mg/LNAA solution is immersed in after taking-up, aseptic water washing is clean, insert loess: in the nutritious bag of rice husk=2:1, regular water spray, weeding, cover the shade net of 65%, temperature 28 DEG C, humidity 75%, and keep ventilated, survival rate 91%.
Embodiment 3
Get the tender blade of mandarin clematis herb children, running water 40min, on superclean bench 10% hydrogen peroxide treatment 8min, aseptic water washing 6 times to noresidue, the mandarin clematis herb blade access N disinfected
6carry out the induction of callus in+6-BA0.5mg/L+2,4-D0.5mg/L+Vc1g/L medium, additional saccharose 30g/L, agar 6.5g/L, pH5.8, half-light photograph, temperature 22 DEG C, the callus derived puts into medium N
6+ 6-BA0.5mg/L+KT0.7mg/L carries out induction cultivation, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, photophase 14h/d, temperature 24 DEG C, cultivate in the differentiation obtained bud seedling access MS+NAA0.15mg/L out, obtain healthy and strong bud seedling, additional saccharose 40g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 24 DEG C, the bud seedling being about 3cm takes out, be immersed in the Cu solion of 0.2% and soak 3min, 1min in the tray containing 20mg/LNAA solution is immersed in after taking-up, aseptic water washing is clean, insert loess: in the nutritious bag of rice husk=2:1, regular water spray, weeding, cover the shade net of 65%, temperature 28 DEG C, humidity 75%, and keep ventilated, survival rate 92%.
Claims (3)
1. a method for quickly breeding for mandarin clematis herb tissue cultures, comprise the acquisition of the aseptic blade of mandarin clematis herb, the induction of callus, the differentiation, culture of rootage etc. of callus, its key step is as follows:
(1) mandarin clematis herb blade is got, to its disinfection;
(2) the mandarin clematis herb blade access N that step (1) was disinfected is got
6the induction of callus is carried out, additional saccharose 30g/L, agar 6.5g/L, pH5.8, half-light photograph, temperature 22 DEG C in+6-BA0.5mg/L+2,4-D0.5mg/L+Vc1g/L medium;
(3) get the callus that step (2) derives and put into medium N
6+ 6-BA0.5mg/L+KT0.7mg/L carries out induction cultivation, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, photophase 14h/d, temperature 24 DEG C;
(4) get in differentiation that step (3) obtains bud seedling access MS+NAA0.1-0.15mg/L out and cultivate, obtain healthy and strong bud seedling, additional saccharose 40g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 24 DEG C;
(5) get the bud seedling that step (4) obtains and carry out field root induction.
2. according to the method for quickly breeding of a kind of mandarin clematis herb tissue cultures according to claim 1, it is characterized in that: the acquisition of the aseptic blade of mandarin clematis herb described in step (1) is, get the blade that mandarin clematis herb children is tender, running water 40min, on superclean bench 10% hydrogen peroxide treatment 8min, aseptic water washing 6 times is to noresidue.
3. according to the method for quickly breeding of a kind of mandarin clematis herb tissue cultures according to claim 1, it is characterized in that: in step (5), the method for root induction is that the bud seedling being about 3cm takes out, and is immersed in the Cu solion of 0.2% and soaks 3min, be immersed in 1min in the tray containing 10-30mg/LNAA solution after taking-up, aseptic water washing is clean, insert loess: in the nutritious bag of rice husk=2:1, regularly spray water, weeding, cover the shade net of 65%, temperature 28 DEG C, humidity 75%, and keep ventilated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410540415.8A CN104255505A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for tissue culture of clematis armandii |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410540415.8A CN104255505A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for tissue culture of clematis armandii |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104255505A true CN104255505A (en) | 2015-01-07 |
Family
ID=52147193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410540415.8A Pending CN104255505A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for tissue culture of clematis armandii |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104255505A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798688A (en) * | 2015-05-12 | 2015-07-29 | 九江学院 | Culture method for akebiaquinata decne seedlings |
| CN108184672A (en) * | 2018-03-07 | 2018-06-22 | 贵州省山地资源研究所 | A kind of Stauntonia latifolia callus from stem segment method for inducing and cultivating |
-
2014
- 2014-10-14 CN CN201410540415.8A patent/CN104255505A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798688A (en) * | 2015-05-12 | 2015-07-29 | 九江学院 | Culture method for akebiaquinata decne seedlings |
| CN108184672A (en) * | 2018-03-07 | 2018-06-22 | 贵州省山地资源研究所 | A kind of Stauntonia latifolia callus from stem segment method for inducing and cultivating |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104206270B (en) | The tissue cultures of a kind of flourishing torch root tuber of aromatic turmeric and rhizome expand method | |
| CN104472355B (en) | A kind of Epimedium sagittatum gets over hibernaculum method for quickly breeding | |
| CN106171978A (en) | A kind of tissue culture and rapid propagation method of P. kingianum | |
| CN104255457A (en) | Rapid propagation method for tissue culture of ilex nitidissima | |
| CN105409771A (en) | Rapid propagation method for Gymnadenia conopsea (L.) R. Br. | |
| CN104206277A (en) | Rapid propagation method for curcuma zedoaria | |
| CN104160966A (en) | Rapid propagation method for Mongolian oak regenerated plant | |
| CN104255505A (en) | Rapid propagation method for tissue culture of clematis armandii | |
| CN104221873A (en) | Fast reproduction method for sessiie siemona root tissue culture | |
| CN103461126A (en) | Rapid propagation method for palm trees | |
| CN107667865B (en) | A kind of Ming River ceratostigma plumbaginoides Bunge subculture quick-breeding method | |
| CN106105980A (en) | A kind of Magnolia liliiflora cuttage and seedling culture method | |
| CN103444551B (en) | To induce the tissue cultures test-tube plantlet preparation method that lamiophlomis rotata seed germination seedling is explant | |
| CN104170734A (en) | Rapid propagation method of burmann cinnamon bark | |
| CN104221877A (en) | Rapid propagation method for culturing tissues of rhizoma acori graminei | |
| CN104255522A (en) | Rapid propagation method for plant regeneration of pyrrosia sheareri (Bak.) Ching | |
| CN104160960A (en) | Rapid propagation method for Mongolian oak | |
| CN105230488B (en) | A kind of Cymbidium lancifolium leaf tissue culture method for quickly breeding | |
| CN104396747B (en) | A kind of Anhui Bulbus Fritillariae Uninbracteatae callus induction propagation method | |
| CN104255508A (en) | Rapid propagation method for tissue culture of momordica cochinchinensis (Lour.) Spreng | |
| CN104255461A (en) | Rapid propagation method of cinnamomum burmannii regeneration plants | |
| CN105830926A (en) | Tissue culture and rapid propagation method of striga asiatica | |
| CN103444549A (en) | Rapid albizia chinensis propagation method | |
| CN104170747A (en) | Rapid propagation method for cultivating hygrophila salicifolia suspension cells | |
| CN104255464A (en) | Method for quickly reproducing common Radix Dolomiaea by tissue culture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150107 |
|
| WD01 | Invention patent application deemed withdrawn after publication |