CN104221877A - Rapid propagation method for culturing tissues of rhizoma acori graminei - Google Patents
Rapid propagation method for culturing tissues of rhizoma acori graminei Download PDFInfo
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- CN104221877A CN104221877A CN201410540502.3A CN201410540502A CN104221877A CN 104221877 A CN104221877 A CN 104221877A CN 201410540502 A CN201410540502 A CN 201410540502A CN 104221877 A CN104221877 A CN 104221877A
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- leaved sweetflag
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Abstract
The invention researches a rapid propagation method for culturing tissues of rhizoma acori graminei. The rapid propagation method comprises the steps of: obtaining the sterile explant of rhizoma acori graminei, inducing axillary buds, propagating cluster buds, strengthening seedlings and rooting culture. According to the rapid propagation method, through research on inducing, propagating and rooting of the rhizoma acori graminei, quality of tissue cultured seedlings of propagation material of the rhizoma acori graminei can be improved, and thereby some theoretical bases and technical supports are established for construction and industrialization of the rapid propagation system of the rhizoma acori graminei seedlings.
Description
Technical field
The present invention relates to the quick-breeding method of grass-leaved sweetflag tissue cultures, belong to plant technology field.
Background technology
Grass-leaved sweetflag,
acorus tatarinowii, also known as acorus graminens soland, mountain calamus, medicine calamus, Araeceae, herbaceos perennial, its rhizome tool smell.Ye Quanyuan, lines up two row, spadix (spadix), and bennet is green, and spathe is lobate.Like dark and damp environment, also can grow under the tree that Yu Midu is larger, but not resistance to solar exposure, otherwise blade can turn yellow.Not drought-resistant, slightly cold-resistant, can open country growth in the Yangtze river basin.Under being common in the thick forest of height above sea level 20-2600 rice, be grown on the other stone of small stream.Produce each provinces and regions on the south the Yellow River, Northeastern India also has to Northern Thailand.There is dampness elimination appetizing, effect of have one's ideas straightened out open-minded phlegm, inducing resuscitation intelligence development, not hungry for gastral cavity ruffian, a mouthful diarrhea of keeping silent, coma epilepsy, forgetful deafness.Regulate the flow of vital energy, invigorate blood circulation, loose wind, dries.Control epilepsy, coma due to blocking of the respiratory system, pyreticosis coma, forgetful, deafness with qi stagnation, ambition is unhappy, stomachache, stomachache, wind-cold-dampness arthralgia, swollen ulcer drug, traumatic injury.Main propagation method adopts Propagation of Rhizomes, and tissue culture technology is applied its Fast-propagation and had very strong superiority.
Summary of the invention
Technical problem to be solved by this invention is a kind of method for quickly breeding of grass-leaved sweetflag tissue cultures, the research of the present invention by inducing grass-leaved sweetflag, breeding, take root, grass-leaved sweetflag propagating materials plantlet in vitro quality can be improved, thus establish certain theoretical foundation and technical support for the foundation of grass-leaved sweetflag quick reproduction technique system and industrialization.
For solving the problems of the technologies described above, the present invention adopts following technical proposal:
Get the excellent current-year branch of grass-leaved sweetflag and the stem with bud that diameter is 0.5cm semi-lignified is explant, clip 2-3cm, every length of tape 1 bud, 1d is preserved in running water, detergent immersion 10min, running water 0.5h, on superclean bench 70% ethanol postincubation 35s, the mercury chloride process 10min of 0.5%, aseptic water washing 5 times, the grass-leaved sweetflag stem with bud access NT+2 sterilized, the induction of axillalry bud is carried out in 4-D0.3mg/L medium, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 2000lx, temperature 22 ± 1 DEG C, the axillalry bud derived is put into medium NT+NAA0.4mg/L+IBA0.5mg/L and is carried out Multiplying culture, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 4000lx, temperature 22 ± 1 DEG C, strong sprout is carried out in bud seedling access medium NT+TDZ0.02mg/L+NAA0.2mg/L+6-BA1.0mg/L after propagation, additional saccharose 40g/L, agar 8.0g/L, pH5.8, illumination 6000lx, temperature 23 ± 2 DEG C, culture of rootage is carried out in bud seedling access medium NT+AC0.1-0.2mg/L+NAA0.1-0.2mg/L+Ag5.0-6.0mg/L after strong sprout, additional saccharose 15g/L, agar 6.0g/L, pH5.8, illumination 8000lx, temperature 25 ± 2 DEG C.
The grass-leaved sweetflag survival rate adopting the present invention to prepare is high, and the cycle is short, and output is large, pollutes little, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Get the excellent current-year branch of grass-leaved sweetflag and the stem with bud that diameter is 0.5cm semi-lignified is explant, clip 2-3cm, every length of tape 1 bud, 1d is preserved in running water, detergent immersion 10min, running water 0.5h, on superclean bench 70% ethanol postincubation 35s, the mercury chloride process 10min of 0.5%, aseptic water washing 5 times, the grass-leaved sweetflag stem with bud access NT+2 sterilized, the induction of axillalry bud is carried out in 4-D0.3mg/L medium, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 2000lx, temperature 22 ± 1 DEG C, the axillalry bud derived is put into medium NT+NAA0.4mg/L+IBA0.5mg/L and is carried out Multiplying culture, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 4000lx, temperature 22 ± 1 DEG C, strong sprout is carried out in bud seedling access medium NT+TDZ0.02mg/L+NAA0.2mg/L+6-BA1.0mg/L after propagation, additional saccharose 40g/L, agar 8.0g/L, pH5.8, illumination 6000lx, temperature 23 ± 2 DEG C, culture of rootage is carried out in bud seedling access medium NT+AC0.1mg/L+NAA0.1mg/L+Ag5.0mg/L after strong sprout, additional saccharose 15g/L, agar 6.0g/L, pH5.8, illumination 8000lx, temperature 25 ± 2 DEG C, rooting rate 95%.
Embodiment 2
Get the excellent current-year branch of grass-leaved sweetflag and the stem with bud that diameter is 0.5cm semi-lignified is explant, clip 2-3cm, every length of tape 1 bud, 1d is preserved in running water, detergent immersion 10min, running water 0.5h, on superclean bench 70% ethanol postincubation 35s, the mercury chloride process 10min of 0.5%, aseptic water washing 5 times, the grass-leaved sweetflag stem with bud access NT+2 sterilized, the induction of axillalry bud is carried out in 4-D0.3mg/L medium, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 2000lx, temperature 22 ± 1 DEG C, the axillalry bud derived is put into medium NT+NAA0.4mg/L+IBA0.5mg/L and is carried out Multiplying culture, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 4000lx, temperature 22 ± 1 DEG C, strong sprout is carried out in bud seedling access medium NT+TDZ0.02mg/L+NAA0.2mg/L+6-BA1.0mg/L after propagation, additional saccharose 40g/L, agar 8.0g/L, pH5.8, illumination 6000lx, temperature 23 ± 2 DEG C, culture of rootage is carried out in bud seedling access medium NT+AC0.2mg/L+NAA0.2mg/L+Ag6.0mg/L after strong sprout, additional saccharose 15g/L, agar 6.0g/L, pH5.8, illumination 8000lx, temperature 25 ± 2 DEG C, rooting rate 96%.
Embodiment 3
Get the excellent current-year branch of grass-leaved sweetflag and the stem with bud that diameter is 0.5cm semi-lignified is explant, clip 2-3cm, every length of tape 1 bud, 1d is preserved in running water, detergent immersion 10min, running water 0.5h, on superclean bench 70% ethanol postincubation 35s, the mercury chloride process 10min of 0.5%, aseptic water washing 5 times, the grass-leaved sweetflag stem with bud access NT+2 sterilized, the induction of axillalry bud is carried out in 4-D0.3mg/L medium, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 2000lx, temperature 22 ± 1 DEG C, the axillalry bud derived is put into medium NT+NAA0.4mg/L+IBA0.5mg/L and is carried out Multiplying culture, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 4000lx, temperature 22 ± 1 DEG C, strong sprout is carried out in bud seedling access medium NT+TDZ0.02mg/L+NAA0.2mg/L+6-BA1.0mg/L after propagation, additional saccharose 40g/L, agar 8.0g/L, pH5.8, illumination 6000lx, temperature 23 ± 2 DEG C, culture of rootage is carried out in bud seedling access medium NT+AC0.2mg/L+NAA0.1mg/L+Ag5.0mg/L after strong sprout, additional saccharose 15g/L, agar 6.0g/L, pH5.8, illumination 8000lx, temperature 25 ± 2 DEG C, rooting rate 98%.
Claims (2)
1. a method for quickly breeding for grass-leaved sweetflag tissue cultures, comprise the acquisition of grass-leaved sweetflag aseptic explant, the induction of axillalry bud, the propagation of Multiple Buds, strong sprout, culture of rootage, its key step is as follows:
(1) grass-leaved sweetflag stem with bud is got, to its disinfection;
(2) induction carrying out axillalry bud in the access of grass-leaved sweetflag stem with bud NT+2, the 4-D0.3mg/L medium that step (1) sterilized is got, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 2000lx, temperature 22 ± 1 DEG C;
(3) get axillalry bud that step (2) derives to put into medium NT+NAA0.4mg/L+IBA0.5mg/L and carry out Multiplying culture, additional saccharose 30g/L, agar 8g/L, pH5.8, illumination 4000lx, temperature 22 ± 1 DEG C;
(4) get in the bud seedling access medium NT+TDZ0.02mg/L+NAA0.2mg/L+6-BA1.0mg/L after step (3) propagation and carry out strong sprout, additional saccharose 40g/L, agar 8.0g/L, pH5.8, illumination 6000lx, temperature 23 ± 2 DEG C;
(5) get in the access of the bud seedling after step (4) strong sprout medium NT+AC0.1-0.2mg/L+NAA0.1-0.2mg/L+Ag5.0-6.0mg/L and carry out culture of rootage, additional saccharose 15g/L, agar 6.0g/L, pH5.8, illumination 8000lx, temperature 25 ± 2 DEG C.
2. according to the method for quickly breeding of a kind of grass-leaved sweetflag tissue cultures according to claim 1, it is characterized in that: grass-leaved sweetflag described in step (1) disinfect as getting the excellent current-year branch of grass-leaved sweetflag and the stem with bud that diameter is 0.5cm semi-lignified is explant, clip 2-3cm, every length of tape 1 bud, preserves 1d, detergent immersion 10min in running water, running water 0.5h, on superclean bench 70% ethanol postincubation 35s, the mercury chloride process 10min of 0.5%, aseptic water washing 5 times.
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| CN201410540502.3A CN104221877A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for culturing tissues of rhizoma acori graminei |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962204A (en) * | 2017-05-24 | 2017-07-21 | 云南省农业科学院花卉研究所 | A kind of tissue culture method for breeding of money Pu |
| CN112106656A (en) * | 2020-09-24 | 2020-12-22 | 安徽农业大学 | Direct organ generation type regeneration method of rhizoma acori graminei |
| CN114938780A (en) * | 2022-06-23 | 2022-08-26 | 章丽 | High-yield and high-quality vegetative propagation technology of rhizoma acori graminei |
-
2014
- 2014-10-14 CN CN201410540502.3A patent/CN104221877A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962204A (en) * | 2017-05-24 | 2017-07-21 | 云南省农业科学院花卉研究所 | A kind of tissue culture method for breeding of money Pu |
| CN106962204B (en) * | 2017-05-24 | 2019-05-28 | 云南省农业科学院花卉研究所 | A kind of tissue culture method for breeding of money Pu |
| CN112106656A (en) * | 2020-09-24 | 2020-12-22 | 安徽农业大学 | Direct organ generation type regeneration method of rhizoma acori graminei |
| CN114938780A (en) * | 2022-06-23 | 2022-08-26 | 章丽 | High-yield and high-quality vegetative propagation technology of rhizoma acori graminei |
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Application publication date: 20141224 |