CN104255477A - Rapid propagation method for tissue culture of sorbaria arborea Schneid. - Google Patents
Rapid propagation method for tissue culture of sorbaria arborea Schneid. Download PDFInfo
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- CN104255477A CN104255477A CN201410463194.9A CN201410463194A CN104255477A CN 104255477 A CN104255477 A CN 104255477A CN 201410463194 A CN201410463194 A CN 201410463194A CN 104255477 A CN104255477 A CN 104255477A
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- false spiraea
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Abstract
The invention provides a rapid propagation method for tissue culture of sorbaria arborea Schneid. The rapid propagation method comprises the steps of inducing stem tips, carrying out propagation on cluster buds, carrying out root induction, exercising seedlings, transplanting, etc. The sorbaria arborea Schneid. obtained by the method is high in yield, less in energy consumption and short in growth cycle, and is not limited by time and space; the rapid propagation method is beneficial to establishment of a complete tissue culture rapid propagation system of sorbaria arborea Schneid.
Description
Technical field
The cultivation of high clump false spiraea group training under the present invention relates to condition of tissue culture, belongs to biological technical field.
Background technology
Highbush pearl mei, < I TranNum = "42" > Sorbaria? Arborea? Schneid. < / I >, rosaceae, deciduous shrubs, up to 6 meters, branches in branchlets cylindric, slightly edges, yellow-green, at a young age slightly stellate hairy or pilose, dark brown when old, glabrous; Winter buds ovoid or nearly oblong, apex obtuse, puce, with several exposed scales, outside is fluff.Winglike compound leaf, vanelets 13-17 piece, connects the long 20-32 centimetre of petiole, micro-by pubescence or without hair; Vanelets is to life, and at a distance of 2.5-3.5 centimetre, lanceolar is oval lanceolar extremely, long 4-9 centimetre, wide 1-3 centimetre, tip is gradually sharp, the wide wedge shape of base portion or circle, and there is heavy sawtooth at edge, upper and lower surface without hair or the starlike fine hair of micro-tool below, pinniform net arteries and veins, lateral vein 20-25 couple, below significantly; Habitat: the foot of the hill, hillside, hillside shrubbery, in the woods of hillside, in the shaw of hillside, limestone hillside, small stream limit, in the shrubbery of small stream limit, dragon spruce border shrubbery, in theropencedrymion.Be distributed in Shaanxi Province, central arid belt in Ningxia, Gansu Province, Qinghai Province, Hubei Province, Hunan Province, Chongqing City, Sichuan Province, Yunnan Province, Tibet Autonomous Region.
Summary of the invention
Technical problem to be solved by this invention is to provide the method for quickly breeding of high clump false spiraea tissue cultures, and output is large, less energy consumption, and growth cycle is short, not by the restriction of Time and place, is conducive to setting up complete high clump false spiraea tissue-culturing rapid propagation system.
For solving the problems of the technologies described above, the present invention adopts following technical proposal:
Select the stem apex that high clump false spiraea children is tender, soak three minutes in bleaching powder, tap water 20 minutes, with the mercuric chloride process 8min of the ethanol postincubation 15s of 70%, 1g/L on superclean bench, aseptic water washing 5 times, be seeded on Miller+ZT2.5-3mg/L+IAA0.05-0.1mg/L+3% sucrose+0.7% agar medium and carry out bud inducement cultivation, condition of culture is intensity of illumination 1000lx, illumination 10h/d, temperature 23 DEG C, humidity 50%., the bud seedling growing two panels true leaf derived is accessed in medium Miller+BA0.3-0.35mg/L+3% sucrose+0.7% agar medium and carry out adventitious buds proliferation cultivation, condition is intensity of illumination 2000lx, illumination 12h/d, temperature 23 DEG C, humidity 50%, Multiplying culture is cultivated and is accessed without root induction in the MS medium of hormone afterwards for 40 days, condition is additional saccharose 2%, agar 0.6%, intensity of illumination 10000lx, illumination 16h/d, temperature 25 DEG C, humidity 90%, after induction a period of time, the plantlet in vitro with bud root is transplanted in the vermiculite having added MS nutrient solution, plastic foil on cover, transplant on outdoor bed after 15 days.
Adopt high clump false spiraea prepared by the present invention, output is large, and less energy consumption, growth cycle is short.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Select the stem apex that high clump false spiraea children is tender, soak three minutes in bleaching powder, tap water 20 minutes, with the mercuric chloride process 8min of the ethanol postincubation 15s of 70%, 1g/L on superclean bench, aseptic water washing 5 times, be seeded on Miller+ZT2.5mg/L+IAA0.05mg/L+3% sucrose+0.7% agar medium and carry out bud inducement cultivation, condition of culture is intensity of illumination 1000lx, illumination 10h/d, temperature 23 DEG C, humidity 50%., the bud seedling growing two panels true leaf derived is accessed in medium Miller+BA0.3mg/L+3% sucrose+0.7% agar medium and carry out adventitious buds proliferation cultivation, condition is intensity of illumination 2000lx, illumination 12h/d, temperature 23 DEG C, humidity 50%, Multiplying culture is cultivated and is accessed without root induction in the MS medium of hormone afterwards for 40 days, condition is additional saccharose 2%, agar 0.6%, intensity of illumination 10000lx, illumination 16h/d, temperature 25 DEG C, humidity 90%, after induction a period of time, the plantlet in vitro with bud root is transplanted in the vermiculite having added MS nutrient solution, plastic foil on cover, transplant after 15 days on outdoor bed, survival rate 89%.
Embodiment 2
Select the stem apex that high clump false spiraea children is tender, soak three minutes in bleaching powder, tap water 20 minutes, with the mercuric chloride process 8min of the ethanol postincubation 15s of 70%, 1g/L on superclean bench, aseptic water washing 5 times, be seeded on Miller+ZT3mg/L+IAA0.1mg/L+3% sucrose+0.7% agar medium and carry out bud inducement cultivation, condition of culture is intensity of illumination 1000lx, illumination 10h/d, temperature 23 DEG C, humidity 50%., the bud seedling growing two panels true leaf derived is accessed in medium Miller+BA0.35mg/L+3% sucrose+0.7% agar medium and carry out adventitious buds proliferation cultivation, condition is intensity of illumination 2000lx, illumination 12h/d, temperature 23 DEG C, humidity 50%, Multiplying culture is cultivated and is accessed without root induction in the MS medium of hormone afterwards for 40 days, condition is additional saccharose 2%, agar 0.6%, intensity of illumination 10000lx, illumination 16h/d, temperature 25 DEG C, humidity 90%, after induction a period of time, the plantlet in vitro with bud root is transplanted in the vermiculite having added MS nutrient solution, plastic foil on cover, transplant after 15 days on outdoor bed, survival rate 90%.
Embodiment 3
Select the stem apex that high clump false spiraea children is tender, soak three minutes in bleaching powder, tap water 20 minutes, with the mercuric chloride process 8min of the ethanol postincubation 15s of 70%, 1g/L on superclean bench, aseptic water washing 5 times, be seeded on Miller+ZT3mg/L+IAA0.1mg/L+3% sucrose+0.7% agar medium and carry out bud inducement cultivation, condition of culture is intensity of illumination 1000lx, illumination 10h/d, temperature 23 DEG C, humidity 50%., the bud seedling growing two panels true leaf derived is accessed in medium Miller+BA0.3mg/L+3% sucrose+0.7% agar medium and carry out adventitious buds proliferation cultivation, condition is intensity of illumination 2000lx, illumination 12h/d, temperature 23 DEG C, humidity 50%, Multiplying culture is cultivated and is accessed without root induction in the MS medium of hormone afterwards for 40 days, condition is additional saccharose 2%, agar 0.6%, intensity of illumination 10000lx, illumination 16h/d, temperature 25 DEG C, humidity 90%, after induction a period of time, the plantlet in vitro with bud root is transplanted in the vermiculite having added MS nutrient solution, plastic foil on cover, transplant after 15 days on outdoor bed, survival rate 87%.
Claims (6)
1. the method for quickly breeding of one kind high clump false spiraea group training, it is characterized in that: the stem apex of high clump false spiraea is sterile-processed, be seeded on Miller+ZT2.5-3mg/L+IAA0.05-0.1mg/L+3% sucrose+0.7% agar medium and carry out bud inducement cultivation, the bud seedling growing two panels true leaf derived is accessed in medium Miller+BA0.3-0.35mg/L+3% sucrose+0.7% agar medium and carry out adventitious buds proliferation cultivation, Multiplying culture is cultivated and is accessed without root induction in the MS medium of hormone afterwards for 40 days, takes out acclimatization and transplants after induction a period of time.
2. the method for quickly breeding of a kind of high clump false spiraea group training according to claim 1, it is characterized in that: described disinfect for: select the stem apex that high clump false spiraea children is tender, soak three minutes in bleaching powder, tap water 20 minutes, with the ethanol postincubation 15s of 70% on superclean bench, the mercuric chloride process 8min of 1g/L, aseptic water washing 5 times.
3. the method for quickly breeding of a kind of high clump false spiraea group training according to claim 1, is characterized in that: the condition of culture of bud inducement is intensity of illumination 1000lx, illumination 10h/d, temperature 23 DEG C, humidity 50%.
4. the method for quickly breeding of a kind of high clump false spiraea group training according to claim 1, is characterized in that: the condition of inducing clumping bud is intensity of illumination 2000lx, illumination 12h/d, temperature 23 DEG C, humidity 50%.
5. the method for quickly breeding of a kind of high clump false spiraea group training according to claim 1, is characterized in that: the condition of root induction is additional saccharose 2%, agar 0.6%, intensity of illumination 10000lx, illumination 16h/d, temperature 25 DEG C, humidity 90%.
6. the plantlet in vitro with bud root is transplanted in the vermiculite having added MS nutrient solution, plastic foil on cover, transplant on outdoor bed after 15 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410463194.9A CN104255477A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method for tissue culture of sorbaria arborea Schneid. |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410463194.9A CN104255477A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method for tissue culture of sorbaria arborea Schneid. |
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| CN104255477A true CN104255477A (en) | 2015-01-07 |
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| CN201410463194.9A Pending CN104255477A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method for tissue culture of sorbaria arborea Schneid. |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900453A (en) * | 2017-02-09 | 2017-06-30 | 天津泰达绿化集团有限公司 | A kind of indoor method for planting of false spiraea |
| CN108040885A (en) * | 2018-01-29 | 2018-05-18 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using cherry stem section |
| CN108124773A (en) * | 2018-01-29 | 2018-06-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using apple stem section |
-
2014
- 2014-09-12 CN CN201410463194.9A patent/CN104255477A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106900453A (en) * | 2017-02-09 | 2017-06-30 | 天津泰达绿化集团有限公司 | A kind of indoor method for planting of false spiraea |
| CN108040885A (en) * | 2018-01-29 | 2018-05-18 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using cherry stem section |
| CN108124773A (en) * | 2018-01-29 | 2018-06-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using apple stem section |
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Application publication date: 20150107 |
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| WD01 | Invention patent application deemed withdrawn after publication |