CN104245723A - 变体蔗糖转运蛋白多肽 - Google Patents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
- C12P7/20—Glycerol
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/412,124 US8686114B2 (en) | 2012-03-05 | 2012-03-05 | Variant sucrose transporter polypeptides |
| US13/412124 | 2012-03-05 | ||
| PCT/US2013/028958 WO2013134167A1 (en) | 2012-03-05 | 2013-03-05 | Variant sucrose transporter polypeptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104245723A true CN104245723A (zh) | 2014-12-24 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380012343.3A Pending CN104245723A (zh) | 2012-03-05 | 2013-03-05 | 变体蔗糖转运蛋白多肽 |
Country Status (10)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114231509A (zh) * | 2021-12-31 | 2022-03-25 | 南京诺云生物科技有限公司 | 一种蔗糖磷酸化酶及葡萄糖基甘油生产工艺 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9017961B2 (en) | 2012-03-05 | 2015-04-28 | E.I. Du Pont De Nemours And Company | Recombinant bacteria comprising novel sucrose transporters |
| CN116710559A (zh) | 2020-12-18 | 2023-09-05 | 因比奥斯公司 | 变体蔗糖渗透酶多肽 |
| WO2024248535A1 (ko) * | 2023-06-02 | 2024-12-05 | 아주대학교산학협력단 | 글리세롤 탈수효소 변이체 및 이의 용도 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110136190A1 (en) * | 2009-12-04 | 2011-06-09 | E. I. Du Pont De Nemours And Company | Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose |
| WO2011154503A1 (de) * | 2010-06-11 | 2011-12-15 | Evonik Degussa Gmbh | Mikrobiologische herstellung von c4-körpern aus saccharose und kohlendioxid |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| ATE452979T1 (de) | 1996-11-13 | 2010-01-15 | Du Pont | Herstellungsverfahren von 1,3-propandiol durch rekombinante organismen |
| JP4570775B2 (ja) | 1997-12-02 | 2010-10-27 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 組換え生物によるグリセロールの産生方法 |
| US7041814B1 (en) | 1998-02-18 | 2006-05-09 | Genome Therapeutics Corporation | Nucleic acid and amino acid sequences relating to Enterobacter cloacae for diagnostics and therapeutics |
| US6610836B1 (en) | 1999-01-29 | 2003-08-26 | Genome Therapeutics Corporation | Nucleic acid amino acid sequences relating to Klebsiella pneumoniae for diagnostics and therapeutics |
| US6514733B1 (en) | 1999-08-18 | 2003-02-04 | E. I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high titer |
| RU2212447C2 (ru) | 2000-04-26 | 2003-09-20 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Штамм escherichia coli - продуцент аминокислоты (варианты) и способ получения аминокислот (варианты) |
| EP1227152A1 (en) | 2001-01-30 | 2002-07-31 | Société des Produits Nestlé S.A. | Bacterial strain and genome of bifidobacterium |
| JP3889650B2 (ja) | 2002-03-28 | 2007-03-07 | 三洋電機株式会社 | 画像処理方法、画像処理装置、コンピュータプログラム、及び記録媒体 |
| EP2374879A3 (en) | 2002-04-22 | 2012-01-11 | E. I. du Pont de Nemours and Company | Promoter and plasmid system for genetic engineering |
| MXPA05003382A (es) | 2002-10-04 | 2005-06-22 | Du Pont | Proceso para la produccion biologica de 1,3-propanodiol con alto rendimiento. |
| US7524660B2 (en) | 2005-05-05 | 2009-04-28 | E.I. Du Pont De Nemours And Company | Utilization of fructose in microbial production strains |
| US20110269183A1 (en) | 2007-12-18 | 2011-11-03 | Korea Advanced Institute Of Science And Technology | Recombinant microorganism having an ability of using sucrose as a carbon source |
| US20110217744A1 (en) | 2008-11-07 | 2011-09-08 | Metabolic Explorer | Use of sucrose as substrate for fermentative production of 1,2-propanediol |
| KR101083136B1 (ko) | 2009-02-13 | 2011-11-11 | 씨제이제일제당 (주) | Ｌ-아미노산 생산용 미생물 및 이를 이용하여 ｌ-아미노산을 생산하는 방법 |
| KR101058894B1 (ko) | 2009-03-03 | 2011-08-23 | 씨제이제일제당 (주) | Ｌ-아미노산 생산 미생물 및 이를 이용한 ｌ-아미노산 생산방법 |
| KR101058893B1 (ko) | 2009-03-03 | 2011-08-23 | 씨제이제일제당 (주) | Ｌ-아미노산 생산 미생물 및 이를 이용한 ｌ-아미노산 생산방법 |
| US20110144377A1 (en) | 2009-06-16 | 2011-06-16 | E. I. Du Pont Nemours And Company | Process for the biological production of 3-hydroxypropionic acid with high yield |
| US8129170B1 (en) | 2010-12-06 | 2012-03-06 | E.I. Du Pont De Nemours And Company | Recombinant bacteria having the ability to metabolize sucrose |
| US8222000B2 (en) | 2010-12-06 | 2012-07-17 | E I Du Pont De Nemours And Company | Recombinant bacteria having the ability to metabolize sucrose |
| US8673602B2 (en) * | 2011-08-16 | 2014-03-18 | E I Du Pont De Nemours And Company | Recombinant bacteria having improved sucrose utilization |
| US8629243B2 (en) * | 2011-08-16 | 2014-01-14 | E I Du Pont De Nemours And Company | Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria |
-
2012
- 2012-03-05 US US13/412,124 patent/US8686114B2/en not_active Expired - Fee Related
-
2013
- 2013-03-05 IN IN6807DEN2014 patent/IN2014DN06807A/en unknown
- 2013-03-05 CA CA2866374A patent/CA2866374A1/en not_active Abandoned
- 2013-03-05 AU AU2013230227A patent/AU2013230227A1/en not_active Abandoned
- 2013-03-05 CN CN201380012343.3A patent/CN104245723A/zh active Pending
- 2013-03-05 JP JP2014561006A patent/JP2015509378A/ja active Pending
- 2013-03-05 EP EP13709703.6A patent/EP2822959A1/en not_active Withdrawn
- 2013-03-05 KR KR20147027720A patent/KR20140135803A/ko not_active Withdrawn
- 2013-03-05 WO PCT/US2013/028958 patent/WO2013134167A1/en active Application Filing
- 2013-03-05 SG SG11201405095QA patent/SG11201405095QA/en unknown
-
2014
- 2014-02-06 US US14/174,469 patent/US20140147899A1/en not_active Abandoned
- 2014-02-06 US US14/174,461 patent/US20140155578A1/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110136190A1 (en) * | 2009-12-04 | 2011-06-09 | E. I. Du Pont De Nemours And Company | Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose |
| WO2011154503A1 (de) * | 2010-06-11 | 2011-12-15 | Evonik Degussa Gmbh | Mikrobiologische herstellung von c4-körpern aus saccharose und kohlendioxid |
Non-Patent Citations (2)
| Title |
|---|
| KNUT JAHREIS，ET AL.: "Adaptation of Sucrose Metabolism in the Escherichia coli Wild-Type Strain EC3132", 《JOURNAL OF BACTERIOLOGY》 * |
| VIVEKA VADYVALOO，ET AL.: "Conservation of Residues Involved in Sugar/H+ Symport by the Sucrose Permease of Escherichia coli Relative to Lactose Permease", 《J. MOL. BIOL.》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114231509A (zh) * | 2021-12-31 | 2022-03-25 | 南京诺云生物科技有限公司 | 一种蔗糖磷酸化酶及葡萄糖基甘油生产工艺 |
| CN114231509B (zh) * | 2021-12-31 | 2023-07-25 | 南京诺云生物科技有限公司 | 一种蔗糖磷酸化酶及葡萄糖基甘油生产工艺 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015509378A (ja) | 2015-03-30 |
| SG11201405095QA (en) | 2014-09-26 |
| US20140155578A1 (en) | 2014-06-05 |
| IN2014DN06807A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 2015-05-22 |
| KR20140135803A (ko) | 2014-11-26 |
| US20130230892A1 (en) | 2013-09-05 |
| US20140147899A1 (en) | 2014-05-29 |
| EP2822959A1 (en) | 2015-01-14 |
| WO2013134167A1 (en) | 2013-09-12 |
| AU2013230227A1 (en) | 2014-08-28 |
| CA2866374A1 (en) | 2013-09-12 |
| US8686114B2 (en) | 2014-04-01 |
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