CN104222480A - Method for utilizing corn ethanol distillation waste water to prepare corn protein peptide - Google Patents
Method for utilizing corn ethanol distillation waste water to prepare corn protein peptide Download PDFInfo
- Publication number
- CN104222480A CN104222480A CN201410529810.6A CN201410529810A CN104222480A CN 104222480 A CN104222480 A CN 104222480A CN 201410529810 A CN201410529810 A CN 201410529810A CN 104222480 A CN104222480 A CN 104222480A
- Authority
- CN
- China
- Prior art keywords
- waste water
- protein peptide
- corn
- distillation waste
- corn protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The method provides for utilizing corn ethanol distillation waste water to prepare corn protein peptide. Specifically, the method comprises removing 60% of the moisture of the ethanol distillation waste water through concentration, adjusting the pH of the concentrated ethanol distillation waste water to 6.5-7.0 and performing uniform mixing; performing sterilization at 105 DEG C for 15 minutes, cooling the materials to 30 DEG C, inoculating 10% of bacillus subtillis liquid seeds, performing stirring, and fermenting the materials for 48 hours at 28-30 DEG C and at a rotating speed of 180 rpm; performing sterilization at 115 DEG C for 15 minutes and then centrifugation; concentrating supernate into a status with 25-30% of solid content and performing spray drying to obtain the corn protein peptide. The corn protein peptide can serve as a feed additive.
Description
Technical field
The present invention relates to a kind of processing method of protein, specifically a kind of method utilizing corn alcohol distillation waste water to prepare corn protein peptide.
Background technology
Often produce 1 ton of alcohol when taking corn as fermenting raw materials production alcohol and about need 3 tons of corns, discharge distillation waste water about 10 tons.About 2 ~ 30,000 mg/L of COD about 3 ~ 50,000 mg/L, BOD of distillation waste water.Corn fermentation is produced in alcohol process, and cornstarch is converted to ethanol and carbon dioxide, and corn contains its nutritional labeling, as protein, fat, fiber etc. are all stayed in distillation waste water.During the fermentation simultaneously, due to the effect of microorganism, in distillation waste water, protein, B family vitamin and amino acid content all increase to some extent than corn, therefore, the solid content of distillation waste water up to 3% ~ 5%, and contains the organic matters such as protein, fat, a small amount of carbohydrate.
At present, the common processing mode of alcohol distilled wastewater be concentrated, dry, energy consumption is huge.If can by the organic matter trans-utilization in distillation waste water, significantly reduce its content of organics, just can saving this enormous expenditure for drying, also can avoid the nutritive loss that causes because of oven dry and air pollution simultaneously, realizing energy-saving and environmental protection, utilize alcohol distilled wastewater efficiency utilization.
Summary of the invention
The invention provides a kind of method utilizing alcohol distilled wastewater to prepare corn protein peptide.
The object of the present invention is achieved like this: get alcohol distillation waste liquid, by the moisture of concentrated removal 60%, adjust pH is to 6.5-7.0, stir, 105 DEG C of sterilizings 15 minutes, are cooled to 30 DEG C, by 10% access bacillus subtilis liquid seeds, stir, 28 DEG C-30 DEG C, under rotating speed 180rpm condition, ferment 48 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, is concentrated into solid content 25-30% again by supernatant, spraying dry, i.e. obtained corn protein peptide.
The cultural method of described bacillus subtilis liquid seeds is: take 5g peptone, 4g glucose, 0.1g potassium dihydrogen phosphate, 0.05g magnesium sulfate, add 90ml water, stir, 121 DEG C of sterilizings 30 minutes, be cooled to 30 DEG C, access bacillus subtilis inclined-plane seed, stirs, 28 DEG C-30 DEG C, under rotating speed 200rpm condition, cultivate 24 hours.
The albumen that corn contains is based on alcohol soluble protein, and alcohol soluble protein is not easily absorbed by organism.The present invention take alcohol distilled wastewater as raw material, by the method for fermentable, the alcohol soluble protein be not easily utilized in waste water is converted into corn protein peptide, substantially increases the trophism of zein, functional.Protein peptides has higher biologically active and nutritive value than the amino acid of equivalent, protein peptides by the free amino acid of animal infiltration rate faster than equivalent, greatly faster than the protein of equivalent.Corn protein peptide can be used as the adding ingredient of feed.Corn protein peptide is applied to feedstuff industry and better can promotes growth of animals or poultry, improve cultivation efficiency, reduce aquaculture cost.
The present invention utilizes alcohol distilled wastewater to produce corn protein peptide, decreases environmental pollution, improves level of comprehensive utilization and the economic benefit of alcohol industry.
Four, specific embodiment
Embodiment one
The cultivation of liquid seeds: take 5g peptone, 4g glucose, 0.1g potassium dihydrogen phosphate, 0.05g magnesium sulfate, add 90mL water, stir, 121 DEG C of sterilizings 30 minutes, are cooled to 30 DEG C, access bacillus subtilis inclined-plane seed, stir, 28 DEG C-30 DEG C, under rotating speed 200rpm condition, cultivate 24 hours, detect qualified after, be bacillus subtilis liquid seeds.
The preparation method of corn protein peptide: take alcohol distilled wastewater 1000g, by concentrated removal 600g moisture, obtain 400g slurries, adjust pH value to 6.5-7.0, stir, 105 DEG C of sterilizings 15 minutes, be cooled to 30 DEG C, access 40mL bacillus subtilis liquid seeds, stirs, 28 DEG C-30 DEG C, under rotating speed 180rpm condition, ferment 48 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, is concentrated into solid content 26% again by supernatant, and spraying dry i.e. obtained corn protein peptide.
Embodiment two
The cultivation of liquid seeds: take 20g peptone, then claim 16g glucose, 0.4g potassium dihydrogen phosphate, 0.2g magnesium sulfate, adds 360mL water, stirs, 121 DEG C of sterilizings 30 minutes, be cooled to 30 DEG C, access bacillus subtilis inclined-plane seed, stirs, 28 DEG C-30 DEG C, under rotating speed 200rpm condition, cultivate 24 hours, detect qualified after, be bacillus subtilis liquid seeds.
The preparation method of corn protein peptide: take alcohol distilled wastewater 5kg, by concentrated removal 3kg moisture, obtain 2kg slurries, adjust pH value to 6.5-7.0, stir, 105 DEG C of sterilizings 15 minutes, be cooled to 30 DEG C, access 200mL bacillus subtilis liquid seeds, stirs, 28 DEG C-30 DEG C, under rotating speed 180rpm condition, ferment 48 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, is concentrated into solid content 28% again by supernatant, and spraying dry i.e. obtained corn protein peptide.
Claims (1)
1. the invention provides a kind of method utilizing alcohol distilled wastewater to prepare corn protein peptide, its feature is as follows: get alcohol distillation waste liquid, by the moisture of concentrated removal 60%, adjust pH, to 6.5-7.0, stirs, 105 DEG C of sterilizings 15 minutes, be cooled to 30 DEG C, by 10% access bacillus subtilis liquid seeds, stir, 28 DEG C-30 DEG C, under rotating speed 180rpm condition, ferment 48 hours; 115 DEG C of sterilizings 15 minutes, centrifugation, is concentrated into solid content 25-30% again by supernatant, spraying dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410529810.6A CN104222480A (en) | 2014-10-10 | 2014-10-10 | Method for utilizing corn ethanol distillation waste water to prepare corn protein peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410529810.6A CN104222480A (en) | 2014-10-10 | 2014-10-10 | Method for utilizing corn ethanol distillation waste water to prepare corn protein peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104222480A true CN104222480A (en) | 2014-12-24 |
Family
ID=52212089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410529810.6A Pending CN104222480A (en) | 2014-10-10 | 2014-10-10 | Method for utilizing corn ethanol distillation waste water to prepare corn protein peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104222480A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070243592A1 (en) * | 2006-04-13 | 2007-10-18 | David Peter R | Compositions and methods for producing fermentation products and residuals |
| CN102492759A (en) * | 2011-11-29 | 2012-06-13 | 山东轻工业学院 | Method for preparing soluble corn peptide from Aspergillus niger fermented maize yellow powder |
| CN103141667A (en) * | 2013-04-08 | 2013-06-12 | 黑龙江省轻工科学研究院 | Method for producing corn protein peptide used for feeding by spent grains liquid of corn alcohol |
| CN103976136A (en) * | 2014-04-30 | 2014-08-13 | 安徽农业大学 | Biogas residue resource reutilization method |
-
2014
- 2014-10-10 CN CN201410529810.6A patent/CN104222480A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070243592A1 (en) * | 2006-04-13 | 2007-10-18 | David Peter R | Compositions and methods for producing fermentation products and residuals |
| CN102492759A (en) * | 2011-11-29 | 2012-06-13 | 山东轻工业学院 | Method for preparing soluble corn peptide from Aspergillus niger fermented maize yellow powder |
| CN103141667A (en) * | 2013-04-08 | 2013-06-12 | 黑龙江省轻工科学研究院 | Method for producing corn protein peptide used for feeding by spent grains liquid of corn alcohol |
| CN103976136A (en) * | 2014-04-30 | 2014-08-13 | 安徽农业大学 | Biogas residue resource reutilization method |
Non-Patent Citations (1)
| Title |
|---|
| 乔建芬: "玉米酒精糟液生产全干燥蛋白饲料（DDGS）", 《山西食品工业》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103931878B (en) | Discarded cassava grain stillage liquid is utilized to prepare the method for high protein feed | |
| CN103621765B (en) | Method for preparing oligopeptide through mixed enzymolysis and fermentation of fish meal processing waste liquid and vegetable proteins | |
| CN105614023B (en) | Fermentation enzymolysis agent for soybean meal fermentation and application thereof | |
| CN103549130B (en) | Method for producing seaweed protein feed through synergistic effect of enzymolysis and fermentation | |
| CN104256057A (en) | Method for preparing feed proteins by utilizing alcohol waste liquor and crop straws | |
| CN103804038B (en) | A kind of method utilizing fermentable edible fungi residues and poultry feather to produce fertilizer | |
| CN104984985B (en) | A kind of processing method for dead livestock and poultry | |
| WO2015090204A1 (en) | Method for producing biological feed additive using cassava residue and ethanol waste mash | |
| CN104212863A (en) | Preparation method of feed-use animal protein peptide | |
| CN104886340A (en) | Process for preparing liquid protein feed by utilizing waste alcoholic fermentation liquor | |
| CN104342462A (en) | Dehydroacetic acid (DHA) feed additive prepared from kitchen waste and preparation method thereof | |
| CN106306361A (en) | Method for preparing biological fermentation feed | |
| CN101617737B (en) | Method for using pineapple peel and slag to prepare high activity dried yeast and ferment feed | |
| CN104839425A (en) | Method for producing protein feed through solid fermentation of tomato waste residues by using bacterium mixture | |
| CN104313079A (en) | Preparation method of monensin premix | |
| CN106036239A (en) | High-protein crab feed additive for raising feed utilization rate | |
| EP2128114A2 (en) | Procedure for obtaining a fertilizing product from beer production waste | |
| CN103976143A (en) | Fermented soybean meal preparation method and fermented soybean meal | |
| CN103834592B (en) | A kind of process municipal sludge microbial starter culture and its preparation method | |
| CN103141667A (en) | Method for producing corn protein peptide used for feeding by spent grains liquid of corn alcohol | |
| CN106165765A (en) | A kind of facilitating digestion absorbs high protein crab feed additive | |
| CN106036240A (en) | High-protein crab feed additive for raising production speed | |
| CN105994940B (en) | Bioactive protein feed and preparation method thereof | |
| CN103224970B (en) | Feeding plant protein peptide preparation method | |
| CN104222480A (en) | Method for utilizing corn ethanol distillation waste water to prepare corn protein peptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141224 |