CN104222092B - A kind of bactericidal composition and preparation and application thereof - Google Patents
A kind of bactericidal composition and preparation and application thereof Download PDFInfo
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- CN104222092B CN104222092B CN201410478743.XA CN201410478743A CN104222092B CN 104222092 B CN104222092 B CN 104222092B CN 201410478743 A CN201410478743 A CN 201410478743A CN 104222092 B CN104222092 B CN 104222092B
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Abstract
The invention discloses a kind of bactericidal composition and preparation and application thereof.This bactericidal composition is made up of cyazofamid, tpn and synergist, and the mass ratio of cyazofamid and tpn is 1:(1 ~ 12.5), the ratio of the quality of described synergist and cyazofamid and tpn quality sum is (1 ~ 3): 10.This bactericidal composition in the formulation mass content is 10% ~ 60%, also comprises the material of following mass fraction in preparation: wetting agent 1% ~ 3%, dispersant 1% ~ 5%, and also comprise agriculturally acceptable defoamer etc., water supplies 100%.Its preparation method is uniformly dispersed at above-mentioned each component mixing, through sand mill grinding, obtains the base-material of particle diameter≤1 μm, then add thickener soln in base-material, namely obtain preparation.Cyazofamid tpn suspending agent no dust pollution of the present invention, environmentally friendly, without bales stained, bin stability is good, and to corps diseases, especially cucumber downy mildew has good control efficiency.
Description
Technical field
The invention belongs to disinfectant use in agriculture field, be specifically related to a kind of bactericidal composition and preparation and application thereof.
Background technology
Crops downy mildew is huge to murrain, and such as: cucumber downy mildew is one of important disease on cucumber production, main harm blade causes leaf margin and crispaturas withered, and serious field a slice is withered and yellow, causes serious economic loss to the normal growth of cucumber.The medicament being mainly used in treating cucumber downy mildew is at present metalaxyl, Propamocarb, white urea cyanogen, tpn etc., and these bactericide use continuously because of long-term, and the generation of resistance is quite serious, therefore in the urgent need to new substitute species.Cyazofamid acts on first bactericide that Mitochondrial electron transmits complex compound position.High control efficiency is had to the root rot of the oomycetes diseases such as downy mildew, epidemic disease and rape section, to the germ that existing medicament produces resistance as Propamocarb, metalaxyl, Fluoxastrobin etc., there is very high activity, but its price is more expensive, user is difficult to bear its high use cost, and single medicament Long-Time Service, easily make germ produce resistance, add the consumption of medicament, not only make cost accounting increase, and increase the weight of environmental pollution.
Summary of the invention
The object of the present invention is to provide a kind of bactericidal composition, between the composition of said composition, the effect of mutual synergy can be played.
Described bactericidal composition is made up of cyazofamid, tpn and synergist, wherein, the mass ratio of described cyazofamid and tpn is 1:(1 ~ 12.5), be preferably 1:(6 ~ 12.5), concrete mass ratio can be 1:1,1:6 and 1:12.5;
Described synergist is selected from following at least one: fat alcohol ethoxyl compound, organic silicon polyether and APG; Described organic silicon polyether is 40cst in the viscosity that 25 DEG C measure.
The ratio of the quality of described synergist and cyazofamid and tpn quality sum is (1 ~ 3): 10, is preferably 2:10.
Described fat alcohol ethoxyl compound is the EmulsogenLCN070 purchased from Clariant Chemical Co., Ltd..
Described organic silicon polyether is the DowCorningSylgard309 purchased from Dow Corning Corporation.
Described APG is the APG1200 purchased from BASF Auxiliary Chemicals Co., Ltd..
Another object of the present invention is to provide a kind of biocide preparation for preventing and treating crops downy mildew of efficient, low cost.
The formulation of described biocide preparation specifically can be suspending agent.
Its composition of described biocide preparation comprises the component of following mass percentage: the water of described bactericidal composition 10% ~ 60%, dispersant 1% ~ 5%, wetting agent 1% ~ 5% and surplus.
Described biocide preparation specifically can be made up of the component of following mass percentage: the water of described bactericidal composition 10% ~ 60%, dispersant 1% ~ 5%, wetting agent 1% ~ 5%, antifreezing agent 3% ~ 5%, preservative 0.1% ~ 0.3%, defoamer 0.1% ~ 0.5%, thickener 0.1 ~ 0.3% and surplus.
The described biocide preparation further preferred component by following mass percentage forms: the water of described bactericidal composition 10% ~ 60%, dispersant 3% ~ 5%, wetting agent 1% ~ 5%, antifreezing agent 3% ~ 5%, preservative 0.1% ~ 0.3%, defoamer 0.3% ~ 0.5%, thickener 0.1 ~ 0.3% and surplus.
Described dispersant is selected from following at least one: phosphate compounds, ethylene oxide-propylene oxide block copolymer, carboxylic acids polymer and Sulfonates compound, described phosphate compounds specifically can be purchased from the SoprophorSC of Luo Diya group, the SoprophorFD purchased from Luo Diya Group Co., Ltd, described ethylene oxide propylene oxide block copolymer specifically can be the PluronicPE6200 purchased from BASF China, and described carboxylic acids polymer specifically can be the Tersperse2700 purchased from Hensel graceful (China) Co., Ltd.
Described wetting agent is selected from following at least one: alkylbenzenesulfonate, APEO and sulphate, described alkylbenzenesulfonate specifically can be neopelex, described APEO specifically can be purchased from the Synperonic91/6 of standing grain major company of Britain, the GenapolX060 purchased from Clariant Chemical Co., Ltd., and described sulphate specifically can be lauryl sodium sulfate and dodecyl sulphate potassium.
Described antifreezing agent is selected from following at least one: ethylene glycol, propane diols, glycerine and urea, is preferably ethylene glycol.
Described preservative is selected from following at least one: benzoic acid, Sodium Benzoate, sorbic acid and sodium sorbate.
Described defoamer is selected from following at least one: organosiloxane, tributyl phosphate and silicone.
Described thickener is selected from following at least one: xanthans, polyvinyl alcohol, POLYPROPYLENE GLYCOL, polyethylene glycol, kaolin, clay, aluminium-magnesium silicate, CMC and organobentonite, is preferably xanthans.
Present invention also offers the preparation method of above-mentioned biocide preparation, comprise the steps:
(1) obtain base-material after above-mentioned bactericidal composition, dispersant, wetting agent, antifreezing agent, preservative, defoamer and deionized water being mixed, and sanded treatment is carried out to base-material;
(2) in the described base-material after sanded treatment of step (1), add thickener, after stirring, obtain described biocide preparation.
In above-mentioned preparation method, in step (1), grain diameter≤1 μm in the base-material after described sanded treatment.
In step (2), described thickener adds as a solution, and in described thickener soln, the mass fraction of thickener is 2% ~ 4%.
The preparation-obtained bactericidal composition of the present invention and the application of biocide preparation in control crops downy mildew also belong to the scope of protection of the invention.
In above-mentioned application, described crops downy mildew is preferably cucumber downy mildew.
Compared with single dose, the biocide preparation prepared by the present invention, has obvious synergistic effect to downy mildew, and also reduces the usage amount of cyazofamid and tpn, has saved cost accounting on the one hand, more friendly to environment on the other hand.Meanwhile, because kind of the active substance cyazofamid of two in preparation has the different germ mechanism of action from tpn, and then delayed germ to the drug-fast generation of cyazofamid, improve drug effect; Simultaneously, inventor tests discovery further, when the size controlling of this microbicide compositions suspending agent is below 1 μm, its control efficiency significantly improves, therefore in suspending agent of the present invention all by size controlling at≤1 μm, meanwhile, by composite to cyazofamid, tpn and synergist three, its final control efficiency significantly improves.In a word, the biocide preparation prepared by the present invention is good without dust and bales stained, environmentally friendly, environmental protection and economy and bin stability, and to multiple kinds of crops disease, especially downy mildew has good control efficiency.
Embodiment
Be described method of the present invention below by specific embodiment, but the present invention is not limited thereto, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Experimental technique described in following embodiment, if no special instructions, is conventional method; Described reagent and biomaterial, if no special instructions, all can obtain from commercial channels.
The present invention is by a large amount of indoor bioassay, screening test has been carried out to the conventional medicament metalaxyl, Propamocarb, white urea cyanogen, tpn etc. of control cucumber downy mildew, find that cyazofamid and tpn carry out compositely having synergistic effect to cucumber downy mildew with certain proportion, by adding synergist, finding that cyazofamid, tpn and synergist three are composite further has significant synergistic effect to cucumber downy mildew.The composite usage amount that can reduce cyazofamid of three, thus reduce cost accounting.On this basis according to the physicochemical property of active component, add suitable adjuvant, after sand milling, be prepared into suspending agent.Described adjuvant comprises dispersant, wetting agent, antifreezing agent, defoamer etc. and is of value to active component at storage and the material stablizing and play drug effect in using, be various compositions that are conventional in pesticidal preparations or permission use, there is no particular restriction, all can obtain from commercial channels.
The former medicine of following each embodiment 95.8% cyazofamid used is purchased from Japanese Ishihara Sangyo Kaisha, Ltd.; The former medicine of 97% tpn is purchased from Jiangsu Lan Feng limited company; Dispersant SoprophorSC is purchased from Luo Diya group, dispersant Tersperse2700 is purchased from Hensel graceful (China) Co., Ltd, dispersant PluronicPE6200 is purchased from BASF China, and dispersant SoprophorFD is purchased from Luo Diya Group Co., Ltd; Wetting agent be lauryl sodium sulfate EMAL30N-S purchased from Kao Corp, wetting agent Synperonic91/6 is purchased from standing grain major company of Britain, and wetting agent GenapolX060 is purchased from Clariant Chemical Co., Ltd.; Synergist fat alcohol ethoxyl compound EmulsogenLCN070 is purchased from Clariant Chemical Co., Ltd., synergist low viscosity organic silicon polyether DowCorningSylgard309 is purchased from Dow Corning Corporation, and synergist APG APG1200 is purchased from BASF Auxiliary Chemicals Co., Ltd..
Embodiment 1 cyazofamid and tpn mixture and the composite indoor joint toxicity measuring to bacterium of downy mildew of cucumber of cyazofamid, tpn and synergist three
1 test objective: this test adopts the method for People's Republic of China (PRC) agricultural industry criteria NY/T1156.7-2006, determine cyazofamid and tpn single dose and under different mixed ratio to the co-toxicity of bacterium of downy mildew of cucumber, also determine the composite rear co-toxicity to bacterium of downy mildew of cucumber of cyazofamid, tpn and synergist three.
2 experimental conditions:
2.1 for examination target bacterium: bacterium of downy mildew of cucumber (Pseudoperonosporacubensis) gathers from FuQing, Fujian Province city Qing Kou.
Bacterium of downy mildew of cucumber (Pseudoperonosporacubensis) used in following embodiment, bibliography: Wang Yan, the molecular detection technology of bacterium of downy mildew of cucumber to the indoor resistance risk assessment of Enestroburin and drug-fast strain studies [D], Beijing, China Agricultural University, 2006,1-61.
The public can obtain from Sino-Agri Leading Biosciences Co., Ltd., and this biomaterial related experiment of the present invention of only attaching most importance to again is used, not can be used as other purposes and uses.
2.2 instrument and equipments: electronic balance (0.1mg), amount storage, triangular flask.Culture dish, liquid-transfering gun, high-pressure sterilizing pot, superclean bench, refrigerator, constant incubator etc.
3 experimental scheme:
3.1 examination materials prepare: cucumber variety (early rich No.), and it is for subsequent use that seedling grows to 6 true leaves; The former medicinal dmso solution of 95.8% cyazofamid is made 1.00 × 10
4the storing solution of mg/L concentration, is placed in 4 DEG C of Refrigerator stores for subsequent use; The former medicinal dmso solution of 97% tpn is made 1.00 × 10
4the storing solution of mg/L concentration, is placed in 4 DEG C of Refrigerator stores for subsequent use.
3.2 test procedures:
3.3.1 the preparation of sporangia suspension: select cause of disease blade, with 4 DEG C of aseptic washing lower blade back side Pseudoperonospora cubensis sporangiums, (concentration is 1 × 10 to be made into suspension
6individual sporangium/ml), deposit for subsequent use for 4 DEG C.
3.3.2 medicament preparation: on the basis of preliminary experiment, by 1.00 × 10
4mg/L cyazofamid and 1.00 × 10
4mg/L tpn liquid mass fraction is drug concentration shown in 0.05% Tween 80 aqueous solution dilution preparation table l, and establishes the 0.05% Tween 80 aqueous solution of not dosing to do blank.
3.3.3 chemicals treatment: medicament that 3.3.2 prepares evenly is sprayed in blade two sides to all moistening, for subsequent use after liquid natural air drying.
3.3.4 inoculation with cultivate: after chemicals treatment 24h, by the sporangia suspension spray inoculation deposited at 4 DEG C at vacuum side of blade, often process 10 strains.Illumination/dark each 12h alternate culture after inoculation, temperature controls at 17 ~ 22 DEG C, and relative moisture is more than 90%.
4 investigation: according to blank incidence, after inoculating during 14d stable disease, classification investigation is carried out to inoculation blade, adopts following stage division:
0 grade: anosis;
1 grade: lesion area account for whole leaf area less than 5%;
3 grades: lesion area accounts for the 6%-10% of whole leaf area;
5 grades: lesion area accounts for the 11%-15% of whole leaf area;
7 grades: lesion area accounts for the 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
The plan of establishment of table 1 reagent agent concentration
5 data statistic analysis:
5.1 computational methods: according to survey data, calculate disease index and the control efficiency of each process.
5.2 statistical analyses: the preventive effect probability value according to each drug concentration logarithm value and correspondence does regression analysis, calculate the toxicity regression curvilinear equation y=a+bx of each medicament to bacterium of downy mildew of cucumber, and half effective inhibition concentration EC
50value and correlation coefficient r.The co-toxicity coefficient method representation that the co-toxicity of mixture preparation adopts Sun Yun abundant:
The theoretical toxicity index TTI=Σ of mixture preparation (percentage of this pharmaceutically active ingredient of the toxicity index of certain medicine × in mixture)
It is synergistic effect during CTC >=120; It is antagonism during CTC≤80; Be addition during 80 < CTC < 120.
6 results and analysis:
6.1 evaluating drugs: result shows (table 2,3,4 and 5), under condition of living body, the former medicine of 95.8% cyazofamid has stronger inhibitory action to bacterium of downy mildew of cucumber, its half effective inhibition concentration is 7.2476mg/L, the former medicine of 97% tpn has certain inhibitory action to bacterium of downy mildew of cucumber, and its half effective inhibition concentration is 46.6736mg/L.The former medicine of cyazofamid and the former medicine of tpn are according to 1:12.5, 1:6, 1:2, 1:1, 2:1, the half effective inhibition concentration of the different proportion mixtures such as 6:1 and 12.5:1 to cucumber downy mildew is respectively 16.4977, 18.3098, 15.8360, 10.4451, 8.5950, 10.0032 and 7.9775mg/L, all show certain fungistatic effect, wherein the former medicine of cyazofamid and the former medicine of tpn are according to 1:12.5, after 1:6 and 1:1 mixture, co-toxicity coefficient CTC is greater than 120, show as synergistic effect, according to 1:2, 2:1, after 6:1 and 12.5:1 mixture, CTC is greater than 80, be less than 120, show as addition." * " is the mean value of 10 strains below.
The former medicine of table 295.8% cyazofamid is to the control efficiency of bacterium of downy mildew of cucumber
| Drug concentration (mg/L) | Blank | 62.5 | 12.5 | 2.5 | 0.5 | 0.1 |
| Disease index * (%) | 41.95 | 13.89 | 19.14 | 24.84 | 28.99 | 34.22 |
| Control efficiency (%) | —— | 66.89 | 54.37 | 40.79 | 30.89 | 18.43 |
The former medicine of table 397% tpn is to the control efficiency of bacterium of downy mildew of cucumber
| Drug concentration (mg/L) | Blank | 312.5 | 62.5 | 12.5 | 2.5 | 0.5 |
| Disease index * (%) | 41.95 | 9.46 | 20.3 | 29.17 | 34.17 | 39.78 |
| Control efficiency (%) | —— | 77.45 | 51.61 | 30.46 | 18.55 | 5.17 |
Table 4 cyazofamid and tpn mixture are to the control efficiency of bacterium of downy mildew of cucumber
Table 5 cyazofamid and tpn mixture are to bacterium of downy mildew of cucumber toxicity test result
| Medicament title | Toxicity regression curvilinear equation (y=a+bx) | Correlation coefficient (r) | EC 50(mg/L) |
| The former medicine of 95.8% cyazofamid | y＝0.4658x+4.5993 | 0.9988 | 7.2476 |
| The former medicine of 98% tpn | y＝0.8092x+3.6493 | 0.9958 | 46.6736 |
| Cyanogen: hundred=1:12.5 | y＝0.4164x+4.4931 | 0.9882 | 16.4977 |
| Cyanogen: hundred=1:6 | y＝0.4339x+4.4521 | 0.9987 | 18.3098 |
| Cyanogen: hundred=1:2 | y＝0.3798x+4.5444 | 0.9798 | 15.8360 |
| Cyanogen: hundred=1:1 | y＝0.3568x+4.6365 | 0.9876 | 10.4451 |
| Cyanogen: hundred=2:1 | y＝0.3851x+4.6402 | 0.9948 | 8.5950 |
| Cyanogen: hundred=6:1 | y＝0.4056x+4.5944 | 0.9827 | 10.0032 |
| Cyanogen: hundred=12.5:1 | y＝0.4190x+4.6222 | 0.9890 | 7.9775 |
Table 6 cyazofamid and tpn mixture are to the joint toxicity measuring result of bacterium of downy mildew of cucumber
Table 7 to add after synergist cyazofamid and tpn mixture to the control efficiency of bacterium of downy mildew of cucumber
Table 8 to add after synergist cyazofamid and tpn mixture to bacterium of downy mildew of cucumber toxicity test result
| Medicament title | Toxicity regression curvilinear equation (y=a+bx) | Correlation coefficient (r) | EC 50(mg/L) |
| The former medicine of 95.8% cyazofamid | y＝0.4658x+4.5993 | 0.9988 | 7.2476 |
| The former medicine of 98% tpn | y＝0.8092x+3.6493 | 0.9958 | 46.6736 |
| Cyanogen: hundred=1:12.5 | y＝0.3655x+4.5997 | 0.9963 | 12.4455 |
| Cyanogen: hundred=1:6 | y＝0.3816x+4.6046 | 0.9799 | 10.8710 |
| Cyanogen: hundred=1:2 | y＝0.3557x+4.6448 | 0.9910 | 9.9644 7 --> |
| Cyanogen: hundred=1:1 | y＝0.3950x+4.6363 | 0.9900 | 8.3299 |
| Cyanogen: hundred=2:1 | y＝0.3687x+4.6715 | 0.9810 | 7.7825 |
| Cyanogen: hundred=6:1 | y＝0.4055x+4.6319 | 0.9816 | 8.0870 |
| Cyanogen: hundred=12.5:1 | y＝0.3806x+4.6689 | 0.9714 | 7.4149 |
Table 9 to add after synergist cyazofamid and tpn mixture to the joint toxicity measuring result of bacterium of downy mildew of cucumber
6.2 conclusions and discussion: this test adopt live body pot-culture method determine cyazofamid and tpn single dose and both according to after different proportion mixture to the live body bacteriostatic activity of cucumber downy mildew, result of the test shows, strengthen along with the raising of drug concentration for examination single dose and the inhibitory action of mixture to bacterium of downy mildew of cucumber, be proportionate, 1:1 pressed by the former medicine of cyazofamid and the former medicine of tpn, 1:2, 1:6, 1:12.5 show as synergistic effect after mixture, and along with the reduction gradually of the former medicine of cyazofamid and the former medicine mass ratio of tpn, synergistic effect after both mixtures is more and more significant, according to 2:1, addition is shown as after 6:1 and 12.5:1 mixture,
In cyazofamid and tpn mixture, adding synergist further, (synergist of interpolation is the fat alcohol ethoxyl compound EmulsogenLCN070 purchased from Clariant Chemical Co., Ltd., the quality of synergist of adding and the ratio of cyazofamid and tpn quality sum are 2:10), compared with the mixture not adding synergist, its synergistic effect significantly strengthens, and its corresponding test data is as shown in table 7,8 and 9.
Embodiment 210% cyazofamid tpn suspending agent
The mass ratio of cyazofamid and tpn is 1:1, content is 10% (pure), the ratio of the quality of synergist fat alcohol ethoxyl compound EmulsogenLCN070 and the quality sum of cyazofamid and tpn is 1:10, wetting agent lauryl sodium sulfate EMAL30N-S is 1%, dispersant SoprophorSC consumption 3%, ethylene glycol 3%, xanthans 0.3%, preservative sodium benzoate are 0.3%, defoamer silicone is 0.5%, deionized water supplies surplus.
Weigh other components in each component except xanthans and appropriate amount of deionized water respectively by above proportioning, mix, obtain the base-material of particle diameter below 1 μm through sand mill grinding, then add the xanthan gum solution prepared in advance, mix, obtain suspending agent.Product checking is carried out according to suspending agent quality control index, technical indicator includes effective component content, persistent foamability, pH scope, suspensibility and heat storage stability etc., its corresponding detection method is as follows: use the high performance liquid chromatograph (Agilent company of the U.S., 1220) of band UV-detector to detect its active constituent content; Its persistent foamability is detected by GB/T28137-2011 agricultural chemicals persistent foamability assay method; Its suspensibility is detected by " method 3 " in GB/T14825-2006; Its heat storage stability is detected by GB/T19136-2003 agricultural chemicals heat storage stability assay method; Its active ingredient resolution ratio is detected by GB/T19136-2003 agricultural chemicals heat storage stability assay method.In each embodiment, the detection method of the performance indications of cyazofamid tpn suspending agent is all carry out as stated above below.
Concrete measurement result is: cyazofamid content is 5.06%, and tpn content is 5.09%, persistent foamability qualified (35ml), pH value 7.6, cyazofamid suspensibility 93.04%, tpn suspensibility 95.12%.Heat storage stability is qualified, active ingredient resolution ratio cyazofamid 1.6%, tpn 2.1%.
Embodiment 330% cyazofamid tpn suspending agent
The mass ratio of cyazofamid and tpn is 1:6, content is 30% (pure), the ratio of the quality of synergist low viscosity organic silicon polyether DowCorningSylgard309 and the quality sum of cyazofamid and tpn is 1:10, wetting agent is neopelex 3%, dispersant Tersperse2700 consumption 5%,, ethylene glycol 5%, xanthans 0.2%, preservative sorbic acid are 0.2%, defoamer tributyl phosphate is 0.5%, deionized water supplies surplus.
Weigh other components in each component except xanthans and appropriate amount of deionized water respectively by above proportioning, mix, obtain the base-material of particle diameter below 1 μm through sand mill grinding, then add the xanthan gum solution prepared in advance, mix, obtain suspending agent.
Concrete measurement result is: cyazofamid content is 4.32%, and tpn content is 25.79%, persistent foamability qualified (25ml), pH value 7.8, cyazofamid suspensibility 94.06%, tpn suspensibility 96.22%.Heat storage stability is qualified, active ingredient resolution ratio cyazofamid 1.8%, tpn 2.6%.
Embodiment 443.2% cyazofamid tpn suspending agent
The mass ratio of cyazofamid and tpn is 1:12.5, content is 43.2% (pure), the ratio of the quality of synergist low viscosity organic silicon polyether DowCorningSylgard309 and the quality sum of cyazofamid and tpn is 2:10, wetting agent Synperonic91/6 is 3%, dispersant PluronicPE6200 consumption 5%,, ethylene glycol 5%, xanthans 0.1%, preservative benzoic acid are 0.1%, defoamer organosiloxane is 0.5%, deionized water supplies surplus.
Weigh other components in each component except xanthans and appropriate amount of deionized water respectively by above proportioning, mix, obtain the base-material of particle diameter below 1 μm through sand mill grinding, then add the xanthan gum solution prepared in advance, mix, obtain suspending agent.The assay method of each performance indications of cyazofamid tpn prepared by embodiment 4 is with embodiment 2.
Concrete measurement result is: cyazofamid content is 3.22%, and tpn content is 40.79%, persistent foamability qualified (35ml), pH value 7.7, cyazofamid suspensibility 94.09%, tpn suspensibility 97.12%.Heat storage stability is qualified, active ingredient resolution ratio cyazofamid 1.5%, tpn 2.9%.
Embodiment 550% cyazofamid tpn suspending agent
The mass ratio of cyazofamid and tpn is 2:3, content is 50% (pure), the ratio of the quality sum of the quality of synergist APG APG1200 and cyazofamid and tpn is 2:10, wetting agent is GenapolX0603%, dispersant SoprophorFD consumption 5%, ethylene glycol 5%, xanthans 0.1%, preservative sorbic acid are 0.1%, defoamer tributyl phosphate is 0.3%, deionized water supplies surplus.
Weigh other components in each component except xanthans and appropriate amount of deionized water respectively by above proportioning, mix, obtain the base-material of particle diameter below 1 μm through sand mill grinding, then add the xanthan gum solution prepared in advance, mix, obtain suspending agent.
Concrete measurement result is: cyazofamid content is 20.11%, and tpn content is 30.16%, persistent foamability qualified (45ml), pH value 7.6, cyazofamid suspensibility 95.19%, tpn suspensibility 95.82%.Heat storage stability is qualified, active ingredient resolution ratio cyazofamid 1.8%, tpn 1.9%.
Embodiment 6 cucumber downy mildew field control effectiveness test
1, reagent agent and process:
Medicament 1:70% thiophanate-methyl wetting powder (Tso Tat Co., Ltd., Japan, local usual medicament) dilutes 1000 times with clear water;
Embodiment 2: embodiment 2 preparation clear water dilutes 1000 times;
Embodiment 3: embodiment 3 preparation clear water dilutes 3000 times;
Embodiment 4: embodiment 4 preparation clear water dilutes 4000 times;
Embodiment 4-1: in embodiment 4-1, each component proportion of preparation is all identical with embodiment 4, only unlike in formulation process through sand mill grinding obtain the base-material that particle diameter is 5 μm, embodiment 4-1 preparation clear water dilutes 4000 times;
Embodiment 4-2: in embodiment 4-2, each component proportion of preparation is all identical with embodiment 4, does not onlyly add synergist unlike in preparation process, and embodiment 4-2 preparation clear water dilutes 4000 times;
Embodiment 5: embodiment 5 preparation clear water dilutes 5000 times;
CK (clear water): blank, only sprays with clear water.
2, test method: test cucumber variety is green No. 3 of Tianjin, and growing way is basically identical, does not execute any agricultural chemicals.6 process are established in test, repeat for 3 times, Gong18Ge community, plot area 10.4m
2, minizone randomized arrangement.Adopt 3WBM-16 type conventional spray device, make each blade tow sides fully moistening, Pcnten-1 yne-4 is even.
3, effect research: 6 strains are fixed, 3d " Invest, Then Investigate " complete stool blade, by following formulae discovery preventive effect in every community:
Disease to refer to after increasing value=dispenser disease index before disease index-dispenser
During investigation, the grade scale of sick level used is:
0 grade: blade is without scab;
1 grade: lesion area accounts for blade area less than 10%;
2 grades: lesion area accounts for blade area 10%-20%;
3 grades: lesion area accounts for blade area 20%-50%;
4 grades: lesion area account for blade area more than 50% or blade withered.
The cucumber downy mildew field control effectiveness test result of the different reagent agent of table 7
Note: in table, data are repeat mean value three times, in table, alphabetical identical person represents that 5% level difference is not remarkable, and alphabetical different person represents that 5% level difference is remarkable.
Result of the test is indicated, embodiment 2,3,4,5 preparation all has good control efficiency to cucumber downy mildew, and preventive effect is all greater than local usual medicament, and embodiment 4 preparation preventive effect is the highest.Simultaneously, the preventive effect of embodiment 4-1 preparation is for embodiment 4 preparation, obvious decline, the present inventor thinks that this phenomenon is greater than 1 μm because of the particle diameter (5 μm) of suspended particulate in embodiment 4-1 preparation, as can be seen here, when controlling particle diameter≤1 μm of suspended particulate in preparation, its preventive effect obviously increases, simultaneously, the preventive effect of embodiment 4-2 preparation is significantly lower than embodiment 4 preparation, the present inventor thinks that this phenomenon is because of the cause not adding synergist in embodiment 4-2 preparation, as can be seen here, synergist add the synergistic effect that can significantly improve in cyazofamid tpn suspending agent between two active components.
Claims (10)
1. a bactericidal composition, described bactericidal composition is made up of cyazofamid, tpn and synergist, wherein, the mass ratio of described cyazofamid and tpn is 1:(1 ~ 12.5), described synergist is selected from following at least one: fat alcohol ethoxyl compound and organic silicon polyether; The ratio of the quality of described synergist and cyazofamid and tpn quality sum is (1 ~ 3): 10.
2. bactericidal composition according to claim 1, is characterized in that: the mass ratio of described cyazofamid and tpn is 1:(6 ~ 12.5), the ratio of the quality of described synergist and cyazofamid and tpn quality sum is 2:10.
3. a biocide preparation, described biocide preparation is made up of the component of following mass percentage: the water of bactericidal composition 10% ~ 60%, dispersant 1% ~ 5%, wetting agent 1% ~ 5%, antifreezing agent 3% ~ 5%, preservative 0.1% ~ 0.3%, defoamer 0.1% ~ 0.5%, thickener 0.1% ~ 0.3% and surplus described in claim 1 or 2.
4. biocide preparation according to claim 3, is characterized in that: the formulation of described biocide preparation is suspending agent.
5. biocide preparation according to claim 3 or 4, is characterized in that: described biocide preparation is made up of the component of following mass percentage: the water of described bactericidal composition 10% ~ 60%, dispersant 3% ~ 5%, wetting agent 1% ~ 5%, antifreezing agent 3% ~ 5%, preservative 0.1% ~ 0.3%, defoamer 0.3% ~ 0.5%, thickener 0.1% ~ 0.3% and surplus.
6. biocide preparation according to claim 3 or 4, is characterized in that: described dispersant is selected from following at least one: phosphate compounds, ethylene oxide-propylene oxide block copolymer, carboxylic acids polymer and Sulfonates compound;
Described wetting agent is selected from following at least one: alkylbenzenesulfonate, APEO and sulphate;
Described antifreezing agent is selected from following at least one: ethylene glycol, propane diols, glycerine and urea;
Described preservative is selected from following at least one: benzoic acid, Sodium Benzoate, sorbic acid and sodium sorbate;
Described defoamer is selected from following at least one: organosiloxane, tributyl phosphate and silicone;
Described thickener is selected from following at least one: xanthans, polyvinyl alcohol, POLYPROPYLENE GLYCOL, polyethylene glycol, kaolin, clay, aluminium-magnesium silicate, CMC and organobentonite.
7. prepare a method for biocide preparation according to any one of claim 3-6, comprise the steps:
(1) obtain base-material after described bactericidal composition, dispersant, wetting agent, antifreezing agent, preservative, defoamer and water being mixed, and sanded treatment is carried out to base-material;
(2) in the described base-material after sanded treatment of step (1), add thickener, after stirring, obtain described biocide preparation.
8. method according to claim 7, is characterized in that: grain diameter≤1 μm in the base-material after described sanded treatment;
Described thickener adds as a solution, and in described thickener soln, the mass fraction of thickener is 2% ~ 4%.
9. the bactericidal composition described in claim 1 or 2 and the application of the biocide preparation according to any one of claim 3-6 in control crops downy mildew.
10. apply according to claim 9, it is characterized in that: described crops downy mildew is cucumber downy mildew.
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| CN107094767A (en) * | 2017-05-31 | 2017-08-29 | 宾阳禾医生农业服务有限公司 | Sterilization synergistic composition containing cyazofamid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101658179A (en) * | 2009-07-22 | 2010-03-03 | 深圳诺普信农化股份有限公司 | Complex bactericidal composition containing cyazofamid |
| CN101700038A (en) * | 2009-11-30 | 2010-05-05 | 青岛星牌作物科学有限公司 | Bactericidal composition containing cyazofamid and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101658179A (en) * | 2009-07-22 | 2010-03-03 | 深圳诺普信农化股份有限公司 | Complex bactericidal composition containing cyazofamid |
| CN101700038A (en) * | 2009-11-30 | 2010-05-05 | 青岛星牌作物科学有限公司 | Bactericidal composition containing cyazofamid and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| 三种杀菌剂防治油菜根肿病技术研究;陈坤荣等;《中国油料作物学报》;20120829;第35卷(第4期);424-427 * |
| 氰霜唑10%悬浮剂防治大白菜根肿病田间药效试验;王新生等;《农药科学与管理》;20110215;第32卷(第2期);54-56 * |
| 烷基多糖苷对草甘膦除草剂的增效作用;于厚春等;《农药》;20080731;第47卷(第7期);532-536 * |
| 烷基多糖苷表面活性剂物理化学性质研究进展;王仲妮等;《日用化学工业》;20020630;第32卷(第3期);47-50 * |
| 非离子型有机硅表面活性剂的结构和制备方法;杜扬等;《化学通报》;20021231;第65卷;1-7 * |
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