CN104221685B - A kind of cotton root system three-D space structure research method directly perceived - Google Patents

Abstract

The invention discloses an intuitive research method for the three-dimensional structure of the cotton root system, preparation of a special root growth box for the study of the three-dimensional structure of the cotton root system, placement and filling of the special root growth box for the study of the three-dimensional structure of the cotton root system, and cotton sowing and management, root washing, root sampling and three-dimensional structure visual research; a special root growth box for the three-dimensional structure research of cotton roots, the size of the growth box is 100cm long, 50cm wide, and 100cm high, and the grid is horizontal and vertical Net, the grid size is 3cm*3cm; a method for making a special root growth box for the study of the three-dimensional structure of the cotton root system. Steel mesh is used as horizontal mesh. The invention is simple and easy to learn, has small sampling workload, saves cost, avoids soil structure damage, and realizes accurate acquisition of three-dimensional space structure and growth data of cotton root system.

Description

An intuitive research method for the three-dimensional space structure of cotton root system

technical field

The invention belongs to the technical field of cotton root system research, and in particular relates to an intuitive research method for the three-dimensional spatial structure of cotton root system.

Background technique

The cotton root system is the main absorption organ for cotton plants to absorb nutrients and water, and is also an important place for the synthesis of various hormones, organic acids, amino acids, etc., and plays an important role in the growth and development, physiological functions and substance metabolism of cotton. Good or bad, directly restricts the growth of the above-ground part. The root system grows in the soil and is widely distributed. The depth of the root system can reach more than 1m, and the root system can grow and expand more than 40cm in the horizontal direction of the soil. At present, the experimental research methods of cotton root system are as follows:

(1) X-ray scanning imaging system: When cotton is grown in a special culture container and culture medium, when it is necessary to study the cotton root system, put the cotton cultivation container into the X-ray scanning imager for scanning and detection, which can be used in all directions , non-destructive and sustainable experimental research on cotton root system, including three-dimensional spatial structure, etc. It is currently the most advanced plant root system experimental research and analysis system. But the disadvantage is that the instrument is bulky, not suitable for the test and analysis of a large number of materials, and it is expensive. A complete set of plant root X-ray scanning imaging system costs about 2 million yuan.

(2) Plant root growth monitoring system: such as CI-600 system and BTC-100 system, pre-buried transparent root canals around field plants (such as: cotton), and inserted the scanning camera into the root canal during the test to scan and camera the roots. The root system around the pipe is analyzed and studied by computer software, which is also a non-destructive root system experimental research method, which can continuously observe the dynamic changes of the plant root system throughout the growing season. But there are also shortcomings. First, a large number of root canals are buried, which changes the soil structure and affects the growth of plant roots, and it is impossible to obtain complete three-dimensional structure data of the root system, and root samples cannot be taken out; 400,000 to 600,000 RMB.

(3) Cultivation methods: including hydroponics, sand culture, potted plants, root bags, etc. Use various media to grow cotton or other crops, and sample the whole pot when it is necessary to study the root system to obtain a complete root system. It is suitable for large-scale experimental research, and the complete root system of the whole cotton plant can be easily obtained, and the cost is low. However, the three-dimensional spatial structure and growth data of cotton roots cannot be obtained.

(4) Root drill sampling method: it is a commonly used method for root system research. The root drill is used to take root samples around the cotton in the field for experimental research. The cotton root system can be drilled at different horizontal positions and different soil depths for analysis. Partial data of the spatial structure of the root system can be obtained, but the data of the three-dimensional spatial structure of the root system of the whole plant or the root system of the colony cannot be obtained.

(5) Field excavation method: According to the needs of experimental research, the whole cotton root system can also be obtained by excavating the cotton root system in the field as a whole. It can also be cut when excavating in the field as needed to study the three-dimensional space structure of the cotton root system. But the disadvantage is that the workload is extremely large, too much soil is excavated, and the workload of root flushing is also particularly large, which is not conducive to experimental research, and can only be used for extensive cutting, and cannot be used to study the three-dimensional spatial structure of cotton roots in detail.

At present, the traditional cotton root experimental research methods have the problems of heavy sampling workload, heavy equipment, high cost, changing soil structure, and inability to obtain three-dimensional structure and growth data of cotton root system.

Contents of the invention

The purpose of the embodiments of the present invention is to provide an intuitive research method for the three-dimensional space structure of the cotton root system, aiming at solving the problems of heavy sampling workload, heavy equipment, high cost, changing soil structure, and inability to obtain the three-dimensional space of the cotton root system in the traditional cotton root system research method. Problems with structure and growth data.

The embodiment of the present invention is realized in this way, an intuitive research method for the three-dimensional structure of the cotton root system. Placement and filling of growth boxes, cotton sowing and management, root washing, root sampling and three-dimensional structure visual research;

The special root growth box prepared for the study of the three-dimensional structure of the cotton root system refers to a special cotton root growth box designed and prepared. There are multiple layers of grids in the box, which can make the cotton roots grow attached to the grids and support them during washing. And fix the cotton root system, the cotton root system can be kept in situ after washing, so as to study its three-dimensional space structure;

The placement and filling of the special root growth box for the three-dimensional structure research of the cotton root system refers to the quantity of the special root growth box selected for the three-dimensional structure research of the cotton root system according to the research needs. The plowing layer soil of the sieve is filled with a special root growth box for the study of the three-dimensional structure of the cotton root system;

The cotton sowing and management refers to planting cotton in a special root growth box prepared for the study of the three-dimensional structure of the cotton root system. Each box can plant 2 rows of cotton with a row spacing of 50-60 cm and 3-5 plants in each row. 10cm, weeding, spraying, fertilization, pruning and other management measures are carried out in accordance with the management measures of cotton high-yield fields.

The said flushing root system refers to that the root growth box is flushed with tap water at different periods of cotton growth according to needs, and the soil is washed away, and the grid supports and fixes the cotton root system, so that the cotton root system can maintain the growth state in situ, which is convenient for visual experimentation Research;

The said root system sampling and three-dimensional structure visual research refers to finally researching the three-dimensional space structure of cotton root system according to actual research needs.

Further, the horizontal net in the special root growth box prepared for the study of the three-dimensional structure of the cotton root system is horizontally fixed in the box, and is used to support and fix the cotton root system, so that after the soil is washed away, the cotton root system can also be restored. Can maintain the original growth state.

Further, in the root system sampling and three-dimensional structure visual research, the special root growth box used for the three-dimensional structure research of cotton root system was laid down, and the cotton root system samples in small cubes were cut one by one from the side of the lower part of the box, and used For the experimental research on the physical and chemical properties of cotton roots, the special root growth box for the study of the three-dimensional structure of cotton roots can obtain root samples in a cube of 3cm*3cm*3cm, and the obtained cotton root data can be used for the study of the three-dimensional structure model of cotton roots build and its validation.

A special root growth box for the study of the three-dimensional structure of cotton roots. The size of the growth box is: 100cm long, 50cm wide, and 100cm high. The grid is horizontal and vertical. The grid size is 3cm*3cm, and the front side The number of cells is 33*33.

Further, the horizontal mesh of the special root growth box for the study of the three-dimensional structure of the cotton root system adopts fine steel wire mesh, and can also be replaced by a glass fiber mesh of the same specification. The fixed spacing of the horizontal mesh can be determined according to the needs of actual experiments. It is 3cm, 6cm, 9cm, etc. For the convenience of being fixed on the vertical net, it can be an integer multiple of the grid size of the vertical net.

Further, the vertical net of the dedicated root growth box for the study of the three-dimensional structure of the cotton root system adopts a thick steel wire mesh, which can study the growth status of the cotton root system in the soil with a minimum height of 3 cm, a length of 3 cm, and a width of 3 cm.

A method for manufacturing a special root growth box for the study of the three-dimensional structure of cotton roots, the manufacturing method steps include welding a rectangular parallelepiped frame with a triangle iron, weaving a thick steel wire mesh as a vertical mesh, and weaving a thin steel mesh as a horizontal mesh;

Described welding cuboid frame with triangle iron refers to adopting 4 long triangle irons of 50cm, 8 long triangle irons of 100cm, welded into a cuboid shelf of high 100cm, long 100cm, wide (thick) 50cm;

The woven thick steel wire mesh used as the vertical mesh refers to the use of steel wires with a diameter of 0.3 to 0.4 cm to weave the thick steel mesh as the vertical mesh of a special root growth box for the study of the three-dimensional structure of the cotton root system, and the mesh size is 3cm*3cm , cut two pieces of 100cm*100cm steel wire mesh and weld them on the front and back of the cuboid shelf respectively, cut two pieces of 50cm*100cm steel mesh and weld them on both sides of the cuboid shelf respectively;

Described weaving fine steel wire mesh as horizontal mesh refers to adopting the steel wire of diameter 0.05cm to weave into the fine steel mesh that grid size is 3cm*3cm, fixes cotton root system as horizontal mesh, cuts the steel mesh of several 50cm*100cm, It is parallel to the top and bottom of the special root growth box used for the study of the three-dimensional structure of the cotton root system and fixed in the root box. The size of the cutting should be slightly larger than 50cm*100cm, so that the thin steel mesh can be fixed on the thick steel mesh. In the special root growth box for the study of the three-dimensional structure of the cotton root system, a horizontal net is fixed every 3cm in turn. From the front of the root growth box, a horizontal net is fixed on each vertical net horizontal line, and the same specification can also be used The steel wire mesh is used as the horizontal mesh.

Further, the dimensions of the rectangular parallelepiped frame welded with triangle irons can be designed into other sizes, and several cotton plants can be planted, and the three-dimensional space structure of the root system can be experimentally studied intuitively, and the cross-growth condition of each cotton root system can also be experimentally studied intuitively. , if only one cotton plant is studied, the size of the root growth box can be designed to be 100 cm high, 50 cm long, and 50 cm wide (thick); 50cm, width (thickness) 50cm.

Further, the braided thick steel wire mesh is used as the vertical mesh to prevent the front, rear, left, and right four steel meshes from expanding outwards or inwards when the horizontal mesh is added and the soil is filled. A "ten"-shaped fixing device is welded on the shelf with an angle iron sheet or a thick steel bar to better fix the steel wire mesh. The upper and lower sides of the rectangular parallelepiped shelf are not welded with steel mesh to facilitate soil filling and cotton planting , study roots, etc.

Effect summary

The unit cost of the special root growth box for the three-dimensional structure research of cotton root system provided by the present invention is within 500 yuan, and can be used repeatedly. After each sampling, only the horizontal net in the box is destroyed, and the box body is intact. When using it next time, It only needs to re-install the fixed horizontal net; the intuitive research method of the three-dimensional structure of cotton root system is low-cost, easy to learn, and easy to be mastered by scientific researchers. After the box is rinsed, the natural state of the growth of the cotton root system can be observed very intuitively, and the three-dimensional spatial structure of the cotton root system can be carefully analyzed and studied. The intuitive research method of the three-dimensional space structure of the cotton root system has a small sampling workload, saves costs, avoids the destruction of the soil structure, and realizes the accurate acquisition of the three-dimensional space structure and growth data of the cotton root system.

Description of drawings

Fig. 1 is the flow chart of the steps of the intuitive research method for the three-dimensional space structure of the cotton root system provided by the embodiment of the present invention;

Fig. 2 is a schematic structural view of a special root growth box for the study of the three-dimensional structure of the cotton root system provided by the embodiment of the present invention;

Fig. 3 is the flow chart of the steps for the preparation of a special root growth box for the study of the three-dimensional structure of cotton roots provided by the embodiment of the present invention;

Fig. 4 is a visual effect diagram of the root system after flushing in the special root growth box for the study of the three-dimensional structure of the cotton root system provided by the embodiment of the present invention.

Detailed ways

In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

As shown in Figure 1, an intuitive research method for the three-dimensional structure of cotton roots, the research method steps include preparing a special root growth box S101 for the study of the three-dimensional structure of the cotton root system, and a special root growth box for the study of the three-dimensional structure of the cotton root system Placement and filling of soil S102, cotton sowing and management S103, root washing S104, root sampling and three-dimensional structure visual research S105;

The preparation of the special root growth box S101 for the study of the three-dimensional structure of the cotton root system refers to the design and preparation of a special cotton root growth box. There are multiple layers of grids in the box, which can make the cotton roots grow. Support and fix the cotton root system, and keep the cotton root system in place after washing, so as to study its three-dimensional space structure;

The placement and filling of the special root growth box for the three-dimensional structure research of the cotton root system S102 refers to selecting the number of special root growth boxes for the three-dimensional structure research of the cotton root system according to the research needs, planting cotton in the box, and using The sieved plow layer soil is used to fill the special root growth box used for the study of the three-dimensional structure of the cotton root system; according to the number of root growth boxes used in the research, a soil pit with a depth of 100 cm is dug in the field, and the depth of the soil pit is the same as the height of the root growth box. Consistent, the bottom of the pit is flat, and the root growth boxes are placed neatly in the pit. Adjacent root growth boxes are separated and wrapped tightly with thick plastic cloth to prevent the roots in the box from growing outwards. Surrounded by soil. Fill the pit well, or place the root growth boxes neatly on a flat soil surface. The adjacent root growth boxes are separated and wrapped tightly with thick plastic sheets, and a slightly larger one is built around them with bricks. The height of the pond is equal to the height of the box, and the gap between the root growth box and the brick wall is filled with soil, and the root growth box is filled with the plowing layer soil sieved through a 0.5-1.0cm sieve. During the filling process, first fill part of the soil, Water and then fill the soil, then water and then fill the soil, repeat this until the root growth box is completely filled with soil, the soil needs to be flush with the surface of the box, the whole process takes several days, fill the soil When planting, it is necessary to mix the organic fertilizer and compound fertilizer used as base fertilizer evenly in the top 40cm of soil. It needs to be placed for another 10-20 days so that the soil in the box can be better settled;

The cotton sowing and management S103 refers to planting cotton in a special root growth box prepared for the study of the three-dimensional structure of the cotton root system. Each box can plant 2 rows of cotton with a row spacing of 50-60 cm and 3 to 5 plants in each row. The plant spacing is 10cm, and management measures such as weeding, spraying, fertilization, and pruning are carried out in accordance with the management measures of high-yield cotton fields. Weeds are pulled out in time, and there are no weeds in the root growth box at any time. Planting a few more cotton plants in each box is for the seedling stage. , Bud stage experimental research, used as a special root growth box for the study of the three-dimensional structure of the cotton root system used in the experimental study of the flowering stage and later, only 2 to 4 cotton plants can be left in each box, and used for the experimental research of wheat-cotton interplanting When compounding the root system of the population, plant 2 to 4 rows of wheat in a root growth box with a row spacing of 15 to 20 cm, and reserve a cotton sowing row. ～5 cotton plants, the distance between the cotton row and the wheat side row is 30cm;

The washing root system S104 refers to washing the root growth box with tap water at different periods of cotton growth according to needs, washing away the soil, and supporting and fixing the cotton root system with the grid, so that the cotton root system can maintain the growth state in situ, which is convenient and intuitive. Experimental research, when washing, use tap water to wash from bottom to top and from outside to inside. Under the physical isolation of thick plastic cloth, all the roots are in the root box. Under the support of the horizontal net, the cleaned cotton roots Being able to maintain the original state of growth in the soil plays a very important role in the study of the growth and three-dimensional space structure of cotton roots;

The root system sampling and three-dimensional structure visual research S105 refers to the research on the three-dimensional space structure of the cotton root system according to the actual research needs. Observe the growth distribution of the cotton root system, and the growth trend of each root system can be clearly observed and described. If there are two or more cotton plants in a box, the interactive growth state of the roots of different cotton plants can be observed. If It is a compound population of wheat-cotton intercropping, which can directly and clearly observe the interactive growth state of wheat root system and cotton root system, which is of great significance for the study of the competition and complementarity of the root system of wheat-cotton intercropping compound population; put the cleaned root growth box in the blue or Under the red background, use a high-resolution digital camera to take multiple photos from different directions and angles, and use the digital camera to record the three-dimensional spatial structure of the cotton root system at the same time. The photos and videos can be analyzed and processed by computer software to draw the cotton root system 3D diagram of the spatial structure.

Further, the horizontal net in the special root growth box S101 prepared for the study of the three-dimensional structure of the cotton root system is horizontally fixed in the box to support the cotton root system, so that after the soil is washed away, the cotton root system can still maintain original growth state.

Further, in the root system sampling and three-dimensional structure visual research S105, the special root growth box used for the three-dimensional structure research of the cotton root system is laid flat, and the cotton root system samples in the small cubes are cut one by one from the lower side of the box body, Used for experimental research on the physical and chemical properties of cotton roots, the special root growth box used for the study of the three-dimensional structure of cotton roots can obtain root samples in a cube of 3cm*3cm*3cm, and the obtained cotton root data can also be used for the three-dimensional structure model of cotton roots research establishment and its validation.

As shown in Figure 2, a special root growth box for the study of the three-dimensional structure of cotton roots, the size of the growth box is: length 100cm, width 50cm, height 100cm, the grid is horizontal and vertical, and the grid size is: 3cm*3cm, the number of front grids is 33*33.

Further, the horizontal net of the special root growth box for the study of the three-dimensional structure of the cotton root system adopts a thin steel wire mesh, and can also be replaced by a glass fiber net of the same specification. The fixed spacing of the horizontal net can be determined according to actual research needs. It is 3cm, 6cm, 9cm, etc. For the convenience of being fixed on the vertical net, it can be an integer multiple of the grid size of the vertical net.

Further, the vertical net of the special root growth chamber for the study of the three-dimensional structure of the cotton root system adopts a thick steel wire mesh, and the cotton root system growth status in the soil with a minimum height of 3 cm, a length of 3 cm, and a width of 3 cm can be tested.

As shown in Figure 3, a method of manufacturing a special root growth box for the study of the three-dimensional structure of cotton roots, the manufacturing method step process includes welding a rectangular parallelepiped frame S301 with a triangle iron, weaving a thick steel wire mesh as a vertical mesh S302, and weaving a fine steel wire mesh As Hengwang S303;

Described rectangular parallelepiped shelf S301 of welding with triangle iron refers to adopting 4 long triangle irons of 50cm, 8 long triangle irons of 100cm, welded into a cuboid shelf with a height of 100cm, a length of 100cm, and a width (thickness) of 50cm;

The woven thick steel mesh used as the vertical mesh S302 refers to the use of steel wires with a diameter of 0.3 to 0.4 cm to weave the thick steel mesh as a vertical mesh for a special root growth box for the study of the three-dimensional structure of the cotton root system, and the mesh size is 3cm* 3cm, cut two pieces of 100cm*100cm steel wire mesh and weld them on the front and back of the cuboid shelf respectively, cut two pieces of 50cm*100cm steel wire mesh and weld them on both sides of the cuboid shelf respectively;

The woven fine steel wire mesh used as the horizontal mesh S303 refers to the use of steel wires with a diameter of 0.05cm to weave into a fine steel mesh with a grid size of 3cm*3cm, which is used as a horizontal mesh to fix the cotton root system, and several sheets of 50cm*100cm steel mesh are cut. , parallel to the upper and lower planes of the special root growth box used for the study of the three-dimensional structure of cotton roots and fixed in the root box. The size of the cutting should be slightly larger than 50cm*100cm, so that the thin steel mesh can be fixed on the thick steel mesh. In the special root growth box used for the study of the three-dimensional structure of the cotton root system, a horizontal net is fixed every 3cm in turn. From the front of the root growth box, a horizontal net is fixed on each vertical net horizontal line, and the same can also be used. Specifications of steel wire mesh, or glass fiber mesh of the same specification as horizontal mesh.

Further, the size of the rectangular parallelepiped shelf S301 welded with triangle iron can be designed to be other sizes, so that several cotton plants can be planted, the three-dimensional space structure of the root system can be intuitively studied, and the cross-growth condition of the root system of each cotton plant can also be visually studied. If only one plant of cotton is studied, the size of the root growth box can be designed to be 100 cm high, 50 cm long, and 50 cm wide (thick); if only the growth of cotton roots before flowering is studied, the size of the root growth box can also be designed to be 50 cm high and 50 cm long , Width (thickness) 50cm.

Further, the braided thick steel wire mesh is used as the vertical mesh S302 in order to prevent the front, rear, left, and right four steel meshes from expanding outwards or compressing inwards when adding a horizontal mesh and filling soil. Weld a "ten"-shaped fixing device with an angle iron sheet or a thick steel bar on the shelf to better fix the steel mesh. The upper and lower sides of the cuboid shelf are not welded with steel mesh to facilitate soil filling and planting Cotton, research roots, etc. working principle

As shown in Figure 1, the steps of an intuitive research method for the three-dimensional structure of cotton roots include the preparation of a special root growth box S101 for the study of the three-dimensional structure of the cotton root system, and the placement of the special root growth box for the study of the three-dimensional structure of the cotton root system. and filling soil S102, cotton seeding and management S103, root washing S104, root sampling and three-dimensional structure visual research S105; the preparation of a special root growth box for the three-dimensional structure of cotton roots S101 refers to the design and preparation of special cotton root growth There are multi-layer grids in the box, which can make the cotton root system attach to the grid when it grows, support and fix the cotton root system when washing, and keep the cotton root system in situ after washing, so as to study its three-dimensional space structure; Placement and filling of special root growth boxes for the study of the three-dimensional structure of the cotton root Cotton is planted in a special root growth box for the study of the three-dimensional structure of the cotton root system, and cotton is planted in the box; the cotton sowing and management S103 refers to planting cotton in the special root growth box for the three-dimensional structure research of the cotton root system prepared for each box. 2 rows of cotton can be planted with a row spacing of 50-60cm, 3-5 plants in each row, and a row spacing of 10cm. Management measures such as weeding, spraying, fertilization, and pruning are carried out according to the management measures for high-yield cotton fields; It is necessary to choose to wash the root growth box with tap water at different periods of cotton growth to wash away the soil. Because the grid supports and fixes the cotton root system, the cotton root system can be maintained in situ growth state, which is convenient for intuitive experimental research; the root system sampling Intuitive research on three-dimensional structure S105 refers to finally researching the three-dimensional spatial structure of cotton root system according to actual research needs.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the protection of the present invention. within range.

Claims (7)

1. a kind of cotton root system three-D space structure research method directly perceived, it is characterised in that the research method steps flow chart includes Prepare for cotton root system three-dimensional structure research special root growth case, special for cotton root system three-dimensional structure research System's growth the putting and banket of case, sowing cotton seed and management, rinse root system, root system sampling is intuitively studied with three-dimensional structure；

The preparation refers to that design prepares dedicated cotton for the special root growth case of cotton root system three-dimensional structure research Root growth case, has multi-layer net in case, cotton root system is attached to when growing on grid, and when flushing supports and fixes cotton Root system, keeps cotton root system home state, to understand three-D space structure, the grid Wei Transverse nets and perpendicular net after flushing；

Putting and banketing for the special root growth case for cotton root system three-dimensional structure research refers to according to research need The quantity of the special root growth case for cotton root system three-dimensional structure research is selected, use is tamped with the topsoil of sieving In the special root growth case of cotton root system three-dimensional structure research, plant cotton in the case；

The sowing cotton seed refers in the ready special root growth for cotton root system three-dimensional structure research with management Plant cotton in case, 2 row cottons can be planted per case, 50~60cm of line-spacing, often 3~5 plants of row, and spacing in the rows 10cm, hoes up weeds, sprays, applies Fertilizer, training management measure are carried out according to cotton yield field management measure；

The flushing root system refers to select as needed in cotton growth different times, and root growth case is rinsed with tap water, Soil is washed away, lattice support simultaneously fixes cotton root system, cotton root system is kept growth in situ state, easy to intuitively study；

Intuitively research refers to it is finally according to practical study needs with three-dimensional structure for the root system sampling, understands cotton root system Three-D space structure；

The growth case size that the cotton root system three-D space structure research method directly perceived uses for：Long 100cm, width 50cm, High 100cm, size of mesh opening are：3cm*3cm, the quantity of front face mesh is 33*33；

The production method steps flow chart of the special root growth case is included with angle iron welding cuboid shelf, braiding crude steel silk screen As perpendicular net, braiding finer wire net as horizontal net；

Described 4, the angle iron referred to angle iron welding cuboid shelf using 50cm long, 8, the angle iron of 100cm long, It is welded into high 100cm, long 100cm, the cuboid shelf of width 50cm；

The braiding crude steel silk screen refers to the braided steel wire crude steel silk screen using 0.3~0.4cm of diameter as perpendicular net, as with In the perpendicular net of the special root growth case of cotton root system three-dimensional structure research, size of mesh opening size is 3cm*3cm, cuts two The steel wire of 100cm*100cm is respectively welded at the front of and behind of cuboid shelf, cuts the steel wire of two 50cm*100cm Net is respectively welded at the both sides of cuboid shelf；

The braiding finer wire net refer to use as horizontal net the braided steel wire of diameter 0.05cm into grid size for The finer wire net of 3cm*3cm, supports and fixed cotton root system as horizontal net, cuts the steel wire of several 50cm*100cm, with Parallel in the special root growth case top and bottom of cotton root system three-dimensional structure research and be fixed in root case, size during cutting should More than 50cm*100cm, in the special root growth case for cotton root system three-dimensional structure research, one is fixed every 3cm successively Horizontal net is opened, in terms of root growth case front, each a horizontal net is fixed on perpendicular net horizontal line, the glass of same size can also be used Glass web makees horizontal net.

2. cotton root system three-D space structure research method directly perceived as claimed in claim 1, it is characterised in that the preparation For the horizontal net in the special root growth case of cotton root system three-dimensional structure research using the form being horizontally fixed in case, it is used for Support and fix cotton root system, so that after soil is removed in flushing, cotton root system can also keep initial growth state.

3. cotton root system three-D space structure research method directly perceived as claimed in claim 1, it is characterised in that the root system Sampling will lie low in intuitively studying with three-dimensional structure for the special root growth case of cotton root system three-dimensional structure research, from Lower box side starts, one by one the cotton root system sample in clip small cubes, the research as cotton root system physicochemical property, Special root growth case minimum for cotton root system three-dimensional structure research can obtain the root system in the cube of 3cm*3cm*3cm Sample, the research that gained cotton root system data are additionally operable to cotton root system three-D space structure model are established and verified.

4. cotton root system three-D space structure research method directly perceived as claimed in claim 1, it is characterised in that described is used for The horizontal net of the special root growth case of cotton root system three-dimensional structure research is using finer wire net or the glass fiber mesh of same size Instead of using, horizontal net constant spacing according to actual tests research it needs to be determined that, be fixed on perpendicular online, the net with perpendicular net for convenience Lattice size is into integral multiple.

5. cotton root system three-D space structure research method directly perceived as claimed in claim 1, it is characterised in that described is used for The perpendicular net of the special root growth case of cotton root system three-dimensional structure research uses crude steel silk screen, and minimum tests high 3cm, long 3cm, width Cotton root system upgrowth situation in 3cm soil.

6. cotton root system three-D space structure research method directly perceived as claimed in claim 1, it is characterised in that the use three Other sizes are can be sized in angle bar welding cuboid shelf, several plants of cottons is planted, intuitively studies root system Three-D space structure and test the intersection upgrowth situation of each strain cotton root system, if only studying 1 plant of cotton, root growth case ruler It is very little to be designed as high 100cm, long 50cm, width 50cm；If only study florescence pervious cotton root system upgrowth situation, root growth case It is designed and sized to high 50cm, long 50cm, width 50cm.

7. cotton root system three-D space structure research method directly perceived as claimed in claim 1, it is characterised in that the braiding Crude steel silk screen is outwards expanded or received inwards as the steel wire of front, rear, left and right four when erecting in net to prevent from adding horizontal net with banketing Pressure, with a criss-cross fixing device, cuboid on angle iron sheet or the welding of crude steel bar on the steel wire and shelf in each face Upper and lower two faces of shelf not welded wire fabric.