CN104207986A - Peony pollen extract, as well as preparation method and application thereof - Google Patents

Abstract

The invention discloses a peony pollen extract, as well as a preparation method and application thereof. The method for preparing the peony pollen extract comprises the following steps: grinding baked peony pollen, and refluxing the ground peony pollen in ethanol; and centrifugally separating the reflux, collecting supernate, evaporating in a rotating manner, and performing freeze-drying in vacuum to obtain the peony pollen extract. The invention further provides a product containing the peony pollen extract as an effective component. The product is especially a skin-care product, and further especially cream or emulsion. The cream contains the peony pollen extract, so that the utilization rate of the peony pollen can be increased; meanwhile, the product has the effects of moistening and wrinkle-resisting and is capable of enhancing the anti-senescence performance by adding the peony pollen extract.

Description

Peony pollen extract and preparation method thereof and application

Technical field

The invention belongs to daily chemicals field, relate to a kind of peony pollen extract and preparation method thereof and application.

Background technology

For the research of Paeonia suffruticosa, be mostly limited to nutritional labeling to its leaf, petal and pistil and the mensuration of medicinal ingredient, and to the rare report of the application and research of peony pollen.Peony pollen also contains the flavone of a great deal of, but rare particular study and application.Paeonia suffruticosa facial cream in the market, is mostly all that plant extract or the essence extracting by interpolation is realized, but effective ingredient in plant could not be fully utilized, therefore fail to make this facial cream to obtain better effect.

Summary of the invention

The object of this invention is to provide a kind of peony pollen extract and preparation method thereof and application.

The method of preparing peony pollen extract provided by the invention, comprises the steps:

After the peony pollen of drying is pulverized, in ethanol, reflux, by the centrifugalize of gained backflow, collect supernatant, the lyophilization of rotary evaporation final vacuum obtains described peony pollen extract.

In said method, in described baking step, temperature is 40-60 DEG C, specifically can be 50 DEG C, and the time is 1-3 hour, specifically can be 2 hours;

Pulverize as micronizing;

After pulverizing, the order number of peony pollen is 100-400 order, is specially 150 orders;

The amount ratio of described peony pollen and ethanol is 1g:40-200mL, is specially 1g:120mL;

In described reflow step, temperature is 40-90 DEG C, is specially 90 DEG C;

Time is 30min-120min, is specially 60min;

In described step with centrifugal separation, centrifugal rotational speed is 3000-15000r/min, is specially 12000r/min;

Time is 3min-15min, is specially 10min;

Centrifugal radius is 5-15cm, is specially 13.5cm.

In addition, the peony pollen extract preparing according to the method described above and the product taking described peony pollen extract as effective ingredient, also belong to protection scope of the present invention.Wherein, described product specifically can be skin care item, is more specifically facial cream or emulsion.

The present invention also provides a kind of compositions, and said composition comprises following each component:

Aforementioned peony pollen extract provided by the invention, EDTA-2NA, allantoin, Dragosine, glycerol, HANSHENGJIAO, hyaluronate sodium, optiphen, solubilizer, SymRelief 100 and water.

In described compositions, the quality percentage composition of each component is respectively:

EDTA-2NA?0.05％-0.1％、

Allantoin 0.3%-0.5%,

Dragosine?0.3％-0.5％、

Glycerol 4%-8%,

HANSHENGJIAO 0.1%-0.5%,

Hyaluronate sodium 0.01-0.1%,

optiphen?0.4％-0.8％、

solubilizer?0.8％-1.5％、

Peony pollen extract 0.3%-1.0%,

SymRelief?100?0.05％-0.08％、

Surplus is described water.

Described compositions is specially by the component of following each quality percentage composition and forms:

EDTA-2NA?0.08％、

Allantoin 0.4%,

Dragosine?0.3％、

Glycerol 5%,

HANSHENGJIAO 0.3%,

Hyaluronate sodium 0.3%,

optiphen?0.6％、

solubilizer?1.0％、

Peony pollen extract 0.8%,

SymRelief?100?0.05％、

Surplus is water.

The pH value of described compositions is 5.5-6.5, is specially 6.0.

The method of the described compositions of preparation provided by the invention, comprise the steps: aforementioned EDTA-2NA, allantoin, glycerol, HANSHENGJIAO to be dissolved in the water and to be heated to 50 DEG C, add again Dragosine and hyaluronate sodium in mixture, add the mixture of solubilizer and SymRelief 100 and optiphen, mix after homogenizing 5min, then add aforementioned peony pollen extract provided by the invention, finally add water and mix, the pH value of regulation system, to 5.5-6.5, obtains described compositions

Use the method for anti-aging face cream of the present invention: clean and skin is done after routine care, the facial cream of getting two soybean grain sizes refers to abdomen as for two forefingers, it is started to pat in full face and cervical region skin from cheek (being dried) equably, then by remainder facial cream flicking in circumference of eyes, note avoiding eye, nose, Kou Chu.

In addition, the peony pollen extract that the invention described above provides or product or compositions have the application in following any one functional product in preparation, also belong to protection scope of the present invention:

1) moisturizing;

2) crease-resistant;

Wherein, described moisturizing is specially the moisture that increases skin surface;

The described crease-resistant roughness that is specially reduction skin is the trend of small structure and fine structure with having the pachylosis of transformation structure.

Compared with prior art, in facial cream provided by the invention, contain peony pollen extract, improved the utilization rate of peony pollen, meanwhile, make product there is moisturizing, crease-resistant effect by adding peony pollen extract, strengthen anti-senescence function.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.Described method is conventional method if no special instructions.Described raw material all can obtain from open commercial sources if no special instructions.

In following embodiment, the Chinese of EDTA-2NA used is disodiumedetate, and CAS No.139-33-can be purchased from Beijing chemical reagents corporation;

The Chinese of Dragosine is carnosine, and CAS No.305-84-0 can be purchased from Jiu Heng scientific & trading Co., Ltd. of Beijing Cigna;

The CAS No.901-44-0 phenoxyethanol of optiphen, can be purchased from Ya Shilan China Investment Ltd.;

Solubilizer is efficient solubilizing agents, can be purchased from De Zhixin Shanghai company limited;

The Chinese of SymRelief 100 is fragrant quick relaxing, can be purchased from Jiu Heng scientific & trading Co., Ltd. of Beijing Cigna;

Embodiment 1, prepare peony pollen extract

Peony pollen is dried after 2 hours at 50 DEG C, weigh, for 5g, after micronizing to order number is 150 orders, in 600mL ethanol in 90 DEG C of backflow 60min, centrifugalize 10min under the condition that is 13.5cm in 12000r/min, centrifugal radius by gained backflow, collects supernatant, and rotary evaporation lyophilization obtain peony pollen extract.

Embodiment 2

Prepare peony pollen anti-aging face cream according to following proportioning:

Component	Percentage by weight
		EDTA-2NA	0.08％
Allantoin	0.40％
		Dragosine	0.30％
Glycerol	5.00％

HANSHENGJIAO	0.30％
		Hyaluronic acid	0.03％
optiphen	0.60％
		solubilizer	1.00％
Peony pollen extract	0.80％
		SymRelief?100	0.05％
Water	Surplus

The preparation method of this facial cream, comprises the following steps:

1) do homework, cleaning equipment instrument, kind number, quantity, the quality of confirmation raw material, accurately take raw material;

2) optiphen, SymRelief 100 and solubilizer are mixed, stir limit and be heated to 50 DEG C;

EDTA-2NA, allantoin, glycerol, HANSHENGJIAO, Dragosine and hyaluronate sodium are mixed, heat while stirring to 50 DEG C;

Above-mentioned two components are mixed to homogenizing 5min;

After being cooled to room temperature, add peony pollen extract to stir, adding water and regulating pH value is 6.0, divides packing through mastic packer, irradiates, after sterilizing and get final product through Co 60.

Embodiment 3, the moisturizing to embodiment 2, crease-resistant facial cream carry out moisturizing evaluation.

Select 15 xerodermatic experimenters, use the facial cream of embodiment 2 after 4 weeks, experimenter's cheek carries out skin moisture content test, the results are shown in Table 1.

Table 1, skin surface moisture statistical result (n=15)

"+": significant difference (p≤0.05), "--": non-significant difference (p>0.5)

The initial average output value of skin surface moisture is 62.53, uses product after 4 weeks, and this value is 77.33, increases by 14.80 than initial value, adopts t inspection, and before and after product uses, relatively skin surface moisture significance increases.This product has moistening effect after using 4 weeks.

Embodiment 4, the moisturizing to embodiment 2, crease-resistant facial cream carry out crease-resistant evaluation.

Select 15 suitable experimenters to use the facial cream of embodiment 2, after 4 weeks, carry out the test of skin coarseness and skin depth frequency, evaluate crease-resistant effect of this facial cream.Skin coarseness the results are shown in Table 2.Skin depth frequency test the results are shown in Table 3.

Table 2, skin coarseness statistical result (n=15)

The initial geometrical mean of skin coarseness parameters R z is 118.8 μ m, uses product after 4 weeks, to reduce by 4.6 μ m, has reduced 3.9%; The initial geometrical mean of Ra is 29.9 μ m, uses product after 4 weeks, to reduce by 1.4 μ m, has reduced 4.8%; Adopt ANOVA-F-Test inspection, skin coarseness parameters R z and all significance minimizings of Ra before using than product after product uses 4 weeks, skin coarseness improves.

Table 3, skin depth distribution frequency (FDD) result (n=15)

* P.o.T[%]: part-structure accounts for the percentage ratio of population structure

After product uses 4 weeks, in skin depth distribution frequency, fine structure increases by 3070, accounts for 13.7% of general construction; Small structure increases by 19881, accounts for general construction 6.7%; Coarse structure reduces 22225, accounts for general construction 18.2%.The distribution frequency of skin fine structure and small structure increases, and coarse structure distribution frequency reduces, the having and weaken the trend that forms tiny wrinkle compared with deep wrinkle of skin.

Above result demonstration, skin coarseness parameters R z and Ra be significance minimizings before and after product uses, and the frequency distribution of fine structure and small structure increases, and the frequency distribution of coarse structure reduces.Comprehensive above each parameter result, this product has anti-wrinkle effect after using 4 weeks.

Claims (10)

1. a method of preparing peony pollen extract, comprises the steps:

After the peony pollen of drying is pulverized, in ethanol, reflux, by the centrifugalize of gained backflow, collect supernatant, the lyophilization of rotary evaporation final vacuum obtains described peony pollen extract.

2. method according to claim 1, is characterized in that: described pulverizing is micronizing;

After pulverizing, the order number of peony pollen is 100-400 order, is specially 100-140 order;

The amount ratio of described peony pollen and ethanol is 1g:40-200mL, is specially 1g:120mL;

In described reflow step, temperature is 40-90 DEG C, is specially 90 DEG C;

Time is 30-120min, is specially 60min;

In described step with centrifugal separation, centrifugal rotational speed is 3000-15000r/min, is specially 12000r/min;

Time is 3min-15min, is specially 10min;

Centrifugal radius is 5cm-15cm, is specially 13.5cm.

3. the peony pollen extract that described in claim 1 or 2, method obtains.

4. taking peony pollen extract described in claim 3 as the product of effective ingredient.

5. product according to claim 5, is characterized in that: described product is skin care item, is specially facial cream or emulsion.

6. a compositions, comprises following each component:

Peony pollen extract, EDTA-2NA, allantoin, Dragosine, glycerol, HANSHENGJIAO, hyaluronate sodium, optiphen, solubilizer, SymRelief 100 and water described in claim 3.

7. compositions according to claim 6, is characterized in that: in described compositions, the quality percentage composition of each component is respectively:

EDTA-2NA?0.05％-0.1％、

Allantoin 0.3%-0.5%,

Dragosine?0.3％-0.5％、

Glycerol 4%-8%,

HANSHENGJIAO 0.1%-0.5%,

Hyaluronate sodium 0.01-0.1%,

optiphen?0.4％-0.8％、

solubilizer?0.8％-1.5％、

Peony pollen extract 0.3%-1.0%,

SymRelief?100?0.05％-0.08％、

Surplus is described water.

8. according to the compositions described in claim 6 or 7, it is characterized in that: the pH value of described compositions is 5.5-6.5, is specially 6.0.

9. prepare the method for the arbitrary described compositions of claim 6-8 for one kind, comprise the steps: EDTA-2NA described in claim 6-8, allantoin, glycerol, HANSHENGJIAO to be dissolved in the water and to be heated to 50 DEG C, add again Dragosine and hyaluronate sodium in mixture, add the mixture of solubilizer and SymRelief 100 and optiphen, mix after homogenizing 5min, add again described peony pollen extract, finally add water and mix, the pH value of regulation system, to 5.5-6.5, obtains described compositions.

10. described in claim 3, described in peony pollen extract or claim 4 or 5, product or the arbitrary described compositions of claim 6-8 have the application in following any one functional product in preparation:

1) moisturizing;

2) crease-resistant;

Wherein, described moisturizing is specially the moisture that increases skin surface;

The described crease-resistant roughness that is specially reduction skin is the trend of small structure and fine structure with having the pachylosis of transformation structure.