Summary of the invention
The present invention is directed to the defect of prior art in Antistress agent, aim to provide a kind of Chinese herbal medicine Antistress agent composition and application thereof.
The present invention is achieved through the following technical solutions:
A kind of Chinese herbal medicine Antistress agent composition, by weight, comprises following component:
A kind of Chinese herbal medicine Antistress agent composition, by weight, preferably includes following component:
A kind of Chinese herbal medicine Antistress agent composition of the present invention also comprises carrier corncob.
Schisandra chinens P.E of the present invention is lignanoid, mass concentration > 98%, Wild jujube seeds extract is Total saponin, mass concentration >=2%, wolfberry fruit extract is LBP-X, mass concentration > 50%, Honegsukle flower P.E is chlorogenic acid, mass concentration > 15%, safflower extract is picrocrocin (C16H26O7), mass concentration > 8%.
Antistress agent of the present invention is prepared by the following method: each material fineness requires: the component of 80% can cross 120 eye mesh screens, and except astragalus polyose, all the other components once fill up, use V-Mixer, mixed at high speed 8 minutes, then add astragalus polyose, continue mixing 4 minutes.
Present invention also offers the application of a kind of Chinese herbal medicine Antistress agent composition as feed addictive, described feed is used for fish culture, and described fingerling class is Pelteobagrus fulvidraco, grass carp or bream.
Described Antistress agent composition is added in an amount of 100 ~ 300g/t in feed.
Beneficial effect of the present invention is: 1) reduce animal to the sensitiveness of stressor, significantly improve or improve animal health status, reduces de-glutinous, the food refusal of aquatic livestock body surface, to wait indefinitely abnormal behaviour steathily; 2) there is significant facilitation to intestinal health, reduce the occurrence probability of aquatic livestock intestines problem; 3) alleviate the stress reaction that the physiology sudden turn of events, feed are bad, infected by microbes, bad environments etc. cause, alleviate the malnutrition that stress cause and production performance reduces, aquatic products quality declines and disease resistance such as to weaken at the bad reaction; 4) improve a poor appetite, promote weightening finish, improve efficiency of feed utilization.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed to the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, according to technical spirit of the present invention to any simple modification made for any of the above embodiments or equivalent variations, all drop in protection scope of the present invention.
One, Application Example
In embodiment, Schisandra chinens P.E is lignanoid, mass concentration > 98%, Wild jujube seeds extract is Total saponin, mass concentration >=2%, wolfberry fruit extract is LBP-X, mass concentration > 50%, Honegsukle flower P.E is chlorogenic acid, mass concentration > 15%, safflower extract is picrocrocin (C16H26O7), mass concentration > 8%.
Embodiment 1
A kind of Chinese herbal medicine Antistress agent composition, with 100g metering, be made up of following point of component: astragalus polyose 5g, Schisandra chinens P.E 10g, Wild jujube seeds extract 4g, wolfberry fruit extract 4g, Honegsukle flower P.E 3g, safflower extract 1g, glutamine dipeptide 7g, stable type VC3g, corncob supply surplus.
Embodiment 2
A kind of Chinese herbal medicine Antistress agent composition, with 500g metering, be made up of following point of component: astragalus polyose 5g, Schisandra chinens P.E 25g, Wild jujube seeds extract 5g, wolfberry fruit extract 5g, Honegsukle flower P.E 5g, safflower extract 1g, glutamine dipeptide 10g, stable type VC5g, corncob supply surplus.
Embodiment 3
A kind of Chinese herbal medicine Antistress agent composition, with 100g metering, be made up of following point of component: astragalus polyose 10g, Schisandra chinens P.E 15g, Wild jujube seeds extract 9g, wolfberry fruit extract 9g, Honegsukle flower P.E 5g, safflower extract 3g, glutamine dipeptide 12g, stable type Vc5g, corncob supply surplus.
Two, experimental study
Selective body heavy phase nearly (5.2 ± 0.4) g, athletic yellow forehead juvenile fish 1200 tail, Random assignment in 12 culturing jars, every cylinder 100 tail.Every day is early, middle and late throws something and feeds 3 times, and to being satiated with food, throw something and feed 30min after terminating, residual bait and ight soil in sucking-off pond.Culture experiment carries out 42d altogether, duration of test water temperature 25.0 ± 1.0 DEG C, pH7.5 ± 0.5, dissolved oxygen >=5mg/L, ammonia nitrogen < 0.2mg/L, chlorine residue < 0.1mg/L.
Feed is made by aquatic livestock nutrition teaching and research room of aquatic products system of Southwestern University.Before formula feed, feedstuff is all pulverized and whole mistake 60 mesh sieve through pulverizer, is uniformly mixed into powder on request, at 30 DEG C of air dry oven inner drying 24h, puts the preservation of 0 DEG C, refrigerator.Chinese herbal medicine resisting stress composition is provided by applicant.
Experiment grouping and resisting stress composition add consumption specifically in table 1:
Grouping tested by table 1 and resisting stress composition adds consumption
Experimental design: Pelteobagrus fulvidraco culture experiment terminates rear stopping feeding 24h.Group 1, takes out 5 tail fishes respectively and puts into fry shipping bags and be filled with pure oxygen and pack in group 2 and group each repetition cylinder of 3 groups; Control group then respectively repeats to take out 10 tails respectively in cylinder, puts into 5 tail Pelteobagrus fulvidracos and is filled with pure oxygen and packs (specifically in table 2), transport 2h with picking-up vehicle in each shipping bags.Immediately by blood sample collection after the anesthesia of fish fast deep after transport.In transportation, picking-up vehicle average speed is 50 ~ 60km/h.
Table 2 resisting stress test grouping
Embodiment 4 composition is on the impact of Pelteobagrus fulvidraco production performance
After Chinese medicine composition adds with 100 ~ 150g/t dosage, the growth performance index of Pelteobagrus fulvidraco is as shown in table 3.As can be seen from the table, herbal composite significantly improves rate of body weight gain and the specific growth rate (P < 0.05) of Pelteobagrus fulvidraco, but without significant difference (P > 0.05) between each group; Protein efficiency also significantly improves because of supplementing of herbal composite, along with the increase of herbal composite magnitude of recruitment, its protein efficiency has further raising trend, group 3 comparatively control group and group 1 is all significantly increased (P < 0.05), feed coefficient is then antipodal trend with protein efficiency, is effectively reduced; Herbal composite supplement to Pelteobagrus fulvidraco survival rate comparatively control group significantly improve (P < 0.05).
Table 3 Chinese medicine composition is on the impact of yellow catfish growing performance
With column data shoulder marking-up parent phase with representing difference not significantly (P > 0.05), letter is different represents significant difference (P < 0.05).Except special circumstances, following table is same.
Embodiment 5 transport stress is on the impact of Pelteobagrus fulvidraco
The change of serum cortisol hormone-content after 1.1 Pelteobagrus fulvidraco transports
21d: after the transport of 2h, except positive controls has 1 tail death, all there is not death in negative control, group 1, group 2 and group 3 groups.In the process of transport, positive controls fish, in oxygen bag, shows dysphoria under the upheaval of ripples, fierce travelling, constantly collides a bag wall, attempt to flee from this environment with head.And organize 1, group 2 and group 3 groups of Pelteobagrus fulvidracos also show the comparatively intense reaction such as the water surface of scurrying out at the transport initial stage, namely continue about 15 ~ 20min restores calm, at the bottom of reposing bag or move about gently.After transport terminates, corticosteroid hormone content detection result shows, the fish serum corticosteroid hormone content of positive controls, group 1, group 2 and group 3 groups comparatively negative control group is significantly increased (P < 0.05), but group 1, group 2 and the group serum cortisol hormone-content reduction (P < 0.05) (table 4) more remarkable in positive controls of 3 groups.
The change of haemocyanin and enzymatic activity after 1.2 Pelteobagrus fulvidraco transports
21d: Pelteobagrus fulvidraco total serum protein (TP) after 2h transport stress and albumin (AP) are measured and shows, group 1, group 2, group 3 and positive controls fish serum TP and AP all comparatively negative control group significantly improve (P < 0.05), but group 1, group 2 and group 3 TP and AP comparatively positive controls significantly reduce (P < 0.05).
The Pelteobagrus fulvidraco serum glutamic pyruvic transminase (GPT) of positive controls and alkaline phosphatase (group T) comparatively negative control group significantly improve (P < 0.05), but the serum GPT of group 2 and group 3 with organize T compared with negative control group without significant difference (P > 0.05).
42d: between each group TP, AP, GPT and group T variation tendency and 21d basically identical.
The comparison of other several content of material in the rear serum of 1.3 Pelteobagrus fulvidraco transport
21d: as can be seen from Table 5, except group 3, the fish blood sugar (GLU) of transport group has remarkable increase (P < 0.05) compared with negative control, but all remarkable in positive controls (P < 0.05).
The serum triglyceride (TG) of positive controls and serum total cholesterol (TC) comparatively negative control group significantly improve (P < 0.05), the serum TG of herbal composite supplementation group and TC then with negative control group without significant difference (P > 0.05).
The variation tendency of 42d:GLU between each group and 21d with; The serum TG of herbal composite supplementation group and TC and negative control group are without significant difference (P > 0.05).
The change of serum cortisol and biochemical indicator before and after table 4 Pelteobagrus fulvidraco transport stress
1.4 Pelteobagrus fulvidracos expose the comparison of other several content of material in rear serum
42d: as can be seen from Table 4, the fish blood sugar (GLU) of exposure group has remarkable increase (P < 0.05) compared with negative control group, but all remarkable in positive controls (P < 0.05).
The serum triglyceride (TG) of positive controls and serum total cholesterol (TC) comparatively negative control group significantly improve (P < 0.05), the serum TG of herbal composite supplementation group and TC then with negative control group without significant difference (P > 0.05).
Table 5 Pelteobagrus fulvidraco expose stress before and after the change of serum cortisol and biochemical indicator
Embodiment 6 bacterium attacks the rear survival rate of poison
As shown in Figure 1, in 7 days that observe, there is not death in the negative control group of attacking poison without Aeromonas hydrophila.All the other each group occurs dead from 2d, except BJ3 group, stops dead after each group to 5d.The Pelteobagrus fulvidraco survival rate of group 1, group 2 and group 3 groups is respectively 73%, 66% and 70%, is all significantly higher than positive controls Pelteobagrus fulvidraco survival rate (50%).
Embodiment 7 composition is on the impact of Growth of Grass Carps Ctenopharyngodon Idellus performance and transport stress
Experimental design: stopped before on-test to raise 24h.Selective body heavy phase nearly (5.2 ± 0.4) g, athletic juvenile fish 300 tail, Random assignment is in 12 culturing jars, and every cylinder 100 tail, specifically divides into groups in table 1.Every day is early, middle and late throws something and feeds 3 times, and to being satiated with food, throw something and feed 30min after terminating, residual bait and ight soil in sucking-off pond.Culture experiment carries out 42d altogether, duration of test water temperature 25.0 ± 1.0 DEG C, pH7.5 ± 0.5, dissolved oxygen >=5mg/L, ammonia nitrogen < 0.2mg/L, chlorine residue < 0.1mg/L.
Grouping tested by table 6
1) on the impact of growth performance
After herbal composite adds with 100g/t dosage, the growth performance index of grass carp is as shown in table 7.As can be seen from the table, herbal composite significantly improves rate of body weight gain and the specific growth rate (P < 0.05) of grass carp; Protein efficiency also significantly improves because of supplementing of herbal composite, and along with the increase of herbal composite magnitude of recruitment, its protein efficiency has further raising trend, and feed coefficient is then antipodal trend with protein efficiency, is effectively reduced; Herbal composite supplement to grass carp survival rate comparatively control group significantly improve (P < 0.05)
The impact of table 7 composition Herba Hyperici elodeoidis fish production performance
Performance assessment criteria |
Control group |
Test group |
Improvement rate |
Grass carp mantissa |
100 |
100 |
- |
First starting weight (g) |
69.2 |
69.9 |
- |
Fish body is heavy (g) eventually |
122.5 |
176.8 |
44.3% |
Rate of body weight gain (%) |
77.6±3.9
B |
153.2±26.9
A |
97.4% |
Specific growth rate (%) |
1.03±0.04
B |
1.65±0.19
A |
60.2% |
Feed coefficient |
1.66±0.07
A |
1.27±0.03
B |
23.5% |
Protein digestibility (%) |
88.0
b |
90.8
a |
3.2% |
Total digestibility (%) |
67.8
b |
71.0
a |
4.7% |
Survival rate (%) |
93.3 |
100 |
7.2% |
2) on the impact of transport stress
As shown in Table 8, after herbal composite adds with 100g/t dosage, the survival rate of grass carp obtains significant raising, be specially transportation survival rate and improve 10%, form motility rate after transport temporarily and improve 13.7%, wherein the improvement of the proportion of goods damageds is the most obvious, reduces 72.4%, greatly reduces cost.
The impact of table 8 composition Herba Hyperici elodeoidis fish transport stress
Performance assessment criteria |
Control group |
Test group |
Improvement rate |
Transportation survival rate (%) |
90
b |
99
a |
10.0% |
Motility rate (%) is formed temporarily after transport |
86.72
b |
98.57
a |
13.7% |
The transport proportion of goods damageds (%) |
4.28
a |
1.18
b |
72.4% |
Embodiment 8 Chinese medicine composition is on the impact on bream growth performance
Experimental design, with embodiment 4, stopped before on-test to raise 24h.Selective body heavy phase nearly (5.2 ± 0.4) g, athletic bream juvenile fish 1200 tail, Random assignment is in 12 culturing jars, and every cylinder 100 tail, specifically divides into groups in table 9.Every day is early, middle and late throws something and feeds 3 times, and to being satiated with food, throw something and feed 30min after terminating, residual bait and ight soil in sucking-off pond.Culture experiment carries out 42d altogether, duration of test water temperature 25.0 ± 1.0 DEG C, pH7.5 ± 0.5, dissolved oxygen >=5mg/L, ammonia nitrogen < 0.2mg/L, chlorine residue < 0.1mg/L.
Grouping tested by table 9
Experimental result: after herbal composite adds with 100 ~ 150g/t dosage, the growth performance index of bream is as shown in table 10.As can be seen from the table, herbal composite significantly improves rate of body weight gain and the specific growth rate (P < 0.05) of bream, but without significant difference (P > 0.05) between each group; Protein efficiency also significantly improves because of supplementing of herbal composite, along with the increase of herbal composite magnitude of recruitment, its protein efficiency has further raising trend, comparatively control group and group 1 group are all significantly increased to organize 3 groups, feed coefficient is then antipodal trend with protein efficiency, is effectively reduced; Herbal composite supplement to bream survival rate comparatively control group significantly improve (P < 0.05).
Table 10 Chinese medicine composition is on the impact of bream growth performance