CN104187206B - A kind of trichogramma nutrient solution and preparation and application thereof - Google Patents
A kind of trichogramma nutrient solution and preparation and application thereof Download PDFInfo
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- CN104187206B CN104187206B CN201410500177.8A CN201410500177A CN104187206B CN 104187206 B CN104187206 B CN 104187206B CN 201410500177 A CN201410500177 A CN 201410500177A CN 104187206 B CN104187206 B CN 104187206B
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- 241000256618 Trichogramma Species 0.000 title claims abstract description 70
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
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- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 22
- 230000017448 oviposition Effects 0.000 claims abstract description 17
- 150000002576 ketones Chemical class 0.000 claims abstract description 16
- 239000006188 syrup Substances 0.000 claims abstract description 14
- 235000020357 syrup Nutrition 0.000 claims abstract description 14
- 210000002969 egg yolk Anatomy 0.000 claims abstract description 10
- 241000411851 herbal medicine Species 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 235000013305 food Nutrition 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 238000000967 suction filtration Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 9
- 240000007594 Oryza sativa Species 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 8
- 235000012907 honey Nutrition 0.000 claims description 8
- 235000009566 rice Nutrition 0.000 claims description 8
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
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- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 6
- 244000105624 Arachis hypogaea Species 0.000 claims description 6
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 6
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- 235000017491 Bambusa tulda Nutrition 0.000 claims description 6
- 240000001548 Camellia japonica Species 0.000 claims description 6
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 6
- 239000011425 bamboo Substances 0.000 claims description 6
- 235000018597 common camellia Nutrition 0.000 claims description 6
- 239000000835 fiber Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000004615 ingredient Substances 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 235000020232 peanut Nutrition 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 239000002351 wastewater Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 244000247747 Coptis groenlandica Species 0.000 claims description 4
- 235000002991 Coptis groenlandica Nutrition 0.000 claims description 4
- 241000576429 Forsythia suspensa Species 0.000 claims description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- 240000003152 Rhus chinensis Species 0.000 claims description 4
- 235000014220 Rhus chinensis Nutrition 0.000 claims description 4
- 239000002775 capsule Substances 0.000 claims description 4
- 241000205585 Aquilegia canadensis Species 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 235000016709 nutrition Nutrition 0.000 abstract description 6
- 208000035240 Disease Resistance Diseases 0.000 abstract description 4
- 238000002372 labelling Methods 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 28
- 241000256844 Apis mellifera Species 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 6
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- 239000011782 vitamin Substances 0.000 description 4
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- 229940088594 vitamin Drugs 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
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- 241001465754 Metazoa Species 0.000 description 2
- 241000186214 Trichogramma dendrolimi Species 0.000 description 2
- 241000406562 Trichogramma ostriniae Species 0.000 description 2
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- 230000035764 nutrition Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 241000255789 Bombyx mori Species 0.000 description 1
- 241001491790 Bupalus piniaria Species 0.000 description 1
- 241000289763 Dasygaster padockina Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 241001477931 Mythimna unipuncta Species 0.000 description 1
- 241000346285 Ostrinia furnacalis Species 0.000 description 1
- 235000019483 Peanut oil Nutrition 0.000 description 1
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- 241000753145 Sitotroga cerealella Species 0.000 description 1
- 241000521156 Trichogramma chilonis Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000010495 camellia oil Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
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- 239000007788 liquid Substances 0.000 description 1
- 239000000312 peanut oil Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
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- 230000028327 secretion Effects 0.000 description 1
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- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of trichogramma nutrient solution and preparation and application thereof, prepare described trichogramma nutrient solution with syrup 20-23 part, fermented bean dregs 15-18 part, tabasheer ketone 8-10 part, yolk powder 5-10 part, wasp dry powder 3-5 part, B B-complex 1-2 part for raw material, in the trichogramma egg-laying season first 1 month, with described nutrient solution feeding trichogramma; Or set up feeder by 10 ~ 12/mu plantation Tanaka, poured described nutrient solution in feeder confession trichogramma in first 1 month of trichogramma egg-laying season and take food.Trichogramma nutrient solution of the present invention utilizes the effective component of Chinese herbal medicine to strengthen the ability of trichogramma larva disease resistance, and for the consumption in trichogramma egg-laying season, in the formula of nutrient solution, increase the nutritional labelings such as fermented bean dregs, after using, effectively can improve the egg laying amount of trichogramma and the survival rate of larva; And its preparation technology is simple, uses conveniently, be suitable for the spread of biological prevention.
Description
Technical field
The invention belongs to insect and raise field, be specifically related to a kind of trichogramma nutrient solution and preparation and application thereof.
Background technology
Trichogramma is a kind of egg parasitoids of Hymenoptera, Trichogramma spp, its breeding relies on adult to be produced in the ovum of the insects such as corn borer, armyworm, bar snout moth's larva, bollworm, cutworm by ovum, larva grows, survives the winter in host egg, nutrition is obtained by the yolk taking food host egg, and pupate in host egg, finally cause host dead.Utilize these growth characteristics of trichogramma, the biological control of multiple agriculture and forestry injurious insect can be widely used in, and remarkable to the control effects of insect, play an important role in proportion of crop planting, grain security production etc.
Trichogramma for biological control mainly contains Trichogramma Ostriniae, trichogramma dendrolimi, Trichogramma chilonis, Tieling trichogramma.At present, the existing report utilizing the insect worm's ovum artificial propagation Trichogramma Ostriniaes such as rice moth, tussah, castor silkworm and pine moth and trichogramma dendrolimi, but be the natural breeding of trichogramma or artificial propagation all more or less there is the problems such as parasitic rate is not high, survival rate is low.The present invention is for solving the problem, propose a kind of trichogramma nutrient solution, the effective component of Chinese herbal medicine is utilized to strengthen the ability of trichogramma larva disease resistance, and for the consumption in trichogramma egg-laying season, in the formula of nutrient solution, increase the nutritional labelings such as fermented bean dregs, after using, effectively can improve the egg laying amount of trichogramma and the survival rate of larva.
Summary of the invention
The object of the present invention is to provide a kind of trichogramma nutrient solution and preparation and application thereof, before the trichogramma egg-laying season 2 ~ 3 months, with prepared nutrient solution feeding adult, the egg laying amount of trichogramma can be improved, strengthen the ability of trichogramma larva disease resistance, significantly improve the survival rate of larva.
For achieving the above object, the present invention adopts following technical scheme:
A kind of trichogramma nutrient solution, its raw material composition is counted by weight: syrup 20-23 part, fermented bean dregs 15-18 part, tabasheer ketone 8-10 part, yolk powder 5-10 part, wasp dry powder 3-5 part, B B-complex 1-2 part; .
Described syrup is that the honey of nectar source brew is diluted with water obtained by Chinese herbal medicine; Described Chinese herbal medicine comprises honeysuckle, the coptis, Chinese gall, the capsule of weeping forsythia.
Described fermented bean dregs is for major ingredient with dregs of beans, the camellia seed dregs of rice, peanut meal, it is pulverized after 4:3:1 mixing, after adding the corn flour mixing of major ingredient weight 15% ~ 20%, add feed fermentation agent and the water of major ingredient weight 0.01 ~ 0.02% again, material moisture is made to be 60 ~ 65%, vapor tight tank is put into, 20 ~ 25 DEG C of lower seal fermentation 2-3 days after mixing thoroughly.
Described tabasheer ketone is after bamboo fiber being processed the waste water filtering in pretreatment process, with mass fraction be 80% ethanol by volume 2 ~ 4:1 mix, repeat extraction 2 ~ 3 times, Extracting temperature 70 ~ 80 DEG C, extracts 1 ~ 2h at every turn, extract suction filtration, filtrate is concentrated into pulpous state and makes.
Described wasp dry powder wasp adult is dried rear pulverizing obtain.
The preparation method of described trichogramma nutrient solution pours in syrup by fermented bean dregs, and after stirring 20min, under 10-20Mpa, pressurization soaks 4-6h, suction filtration; Filtrate adds tabasheer ketone, yolk powder, B B-complex mixing, obtained described trichogramma nutrient solution.
The application of described trichogramma nutrient solution is in the trichogramma egg-laying season first 1 month, with described nutrient solution feeding trichogramma; Or set up feeder by 10 ~ 12/mu plantation Tanaka, poured described nutrient solution in feeder confession trichogramma in first 1 month of trichogramma egg-laying season and take food.
Described plantation field comprises corn planting field, cotton planting field, Rice Cropping field;
Described feeder is made up of support and drip pan, and its support is formed by connecting by " X " shape mutually by least 3 rods or iron.
Remarkable advantage of the present invention is:
(1) the multiple Chinese herbal medicine such as honeysuckle, the coptis, Chinese gall, the capsule of weeping forsythia all has the effects such as antibacterial, antiviral, the honey led to for nectar source with it on the basis having honey medicinal efficacy, also containing effect corresponding to its nectariferous plant.Therefore, be that honey that nectar source is led to is equipped with tabasheer ketone and prepares trichogramma nutrient solution by these Chinese herbal medicines, can strengthen trichogramma adult and hatch the disease resistance ability of larva.
(2) higher thick protein is contained in dregs of beans, it is a kind of conventional animal feed, but containing some incitants in undressed dregs of beans, harmful effect can be produced to digesting and assimilating of animal, the small active peptides in a large number with unique physiologically active can be produced after being fermented, be conducive to digesting and assimilating of nutriment.The present invention is according to the edibility characteristic of trichogramma, fermented bean dregs is prepared for raw material with dregs of beans, the camellia seed dregs of rice, peanut meal, not only can the kind of the nutritional labeling such as protein, trace element in abundant nutrition liquid, soybean oil, camellia oil, peanut oil also can be effectively utilized to squeeze rear remaining residue, reduce the discharge of industrial waste, meanwhile, fermented bean dregs is soaked in syrup and makes the effective stripping of its nutritional labeling, also facilitate trichogramma to suck.
(3) the tabasheer ketone that feed formula of the present invention is used forms with remaining waste water filtering, extraction in bamboo fiber processing pretreatment process, not only can realize recycling of industrial wastewater, also can reduce the production cost of feed formula of the present invention, improve the output of tabasheer ketone.
(4) also add yolk powder, wasp dry powder, vitamin in the formula of trichogramma nutrient solution of the present invention, the secretion of trichogramma hormone in vivo can be promoted, supplement the consumption in trichogramma egg-laying season, effectively improve egg laying amount.
(5) raw material sources of trichogramma nutrient solution of the present invention are extensive, and preparation technology is simple, and use conveniently, are suitable for spread.
Detailed description of the invention
More being convenient to make content of the present invention understand, below in conjunction with detailed description of the invention, technical solutions according to the invention are described further, but the present invention being not limited only to this.
embodiment 1
A kind of trichogramma nutrient solution, its preparation comprises the following steps:
1) preparation of syrup: be diluted with water by coptis honey, is mixed with the sugar aqueous solution that mass fraction is 5%;
2) preparation of fermented bean dregs: pulverize after dregs of beans 2kg, camellia seed dregs of rice 1.5kg, peanut meal 0.5kg mixing, after adding the corn flour mixing of 0.6kg, then add the agent of 0.4g feed fermentation and water, make material moisture be 60%, put into vapor tight tank after mixing thoroughly, 25 DEG C of lower seals ferment 2 days;
3) preparation of tabasheer ketone: after bamboo fiber being processed the waste water filtering in pretreatment process, with mass fraction be 80% ethanol by volume 1:2 mix, repeat extraction 3 times, Extracting temperature 70 DEG C, extracts 2h at every turn, extract suction filtration, filtrate is concentrated into pulpous state and makes;
4) preparation of wasp dry powder: wasp adult is dried rear pulverizing, crosses 100 mesh sieves and obtains;
5) preparation of nutrient solution: 1.5kg fermented bean dregs is poured in 2.0kg syrup, after stirring 20min, under 10Mpa, pressurization soaks 4h, suction filtration; Filtrate adds 0.8kg tabasheer ketone, 0.5kg yolk powder, 0.3kg wasp dry powder, the mixing of 0.1kg vitamin, obtained described trichogramma nutrient solution.
By obtained trichogramma nutrient solution in first 1 month of trichogramma egg-laying season feeding trichogramma, its parasitic rate, eclosion rate, single ovum containing honeybee amount, lopsided honeybee rate test result as follows:
embodiment 2
A kind of trichogramma nutrient solution, its preparation comprises the following steps:
1) preparation of syrup: be diluted with water by Chinese gall honey, is mixed with the sugar aqueous solution that mass fraction is 8%;
2) preparation of fermented bean dregs: pulverize after dregs of beans 2kg, camellia seed dregs of rice 1.5kg, peanut meal 0.5kg mixing, after adding the corn flour mixing of 0.72kg, then add the agent of 0.4g feed fermentation and water, make material moisture be 63%, put into vapor tight tank after mixing thoroughly, 22 DEG C of lower seals ferment 2 days;
3) preparation of tabasheer ketone: after bamboo fiber being processed the waste water filtering in pretreatment process, with mass fraction be 80% ethanol by volume 1:3 mix, repeat extraction 2 times, Extracting temperature 75 DEG C, extracts 1.5h at every turn, extract suction filtration, filtrate is concentrated into pulpous state and makes;
4) preparation of wasp dry powder: wasp adult is dried rear pulverizing, crosses 100 mesh sieves and obtains;
5) preparation of nutrient solution: 1.6kg fermented bean dregs is poured in 2.1kg syrup, after stirring 20min, under 10Mpa, pressurization soaks 5h, suction filtration; Filtrate adds 0.9kg tabasheer ketone, 0.8kg yolk powder, 0.4kg wasp dry powder, the mixing of 0.2kg vitamin, obtained described trichogramma nutrient solution.
By obtained trichogramma nutrient solution in first 1 month of trichogramma egg-laying season feeding trichogramma, its parasitic rate, eclosion rate, single ovum containing honeybee amount, lopsided honeybee rate test result as follows:
embodiment 3
A kind of trichogramma nutrient solution, its preparation comprises the following steps:
1) preparation of syrup: be diluted with water by capsule of weeping forsythia honey, is mixed with the sugar aqueous solution that mass fraction is 10%;
2) preparation of fermented bean dregs: pulverize after dregs of beans 2kg, camellia seed dregs of rice 1.5kg, peanut meal 0.5kg mixing, after adding the corn flour mixing of 0.8kg, then add the agent of 0.8g feed fermentation and water, make material moisture be 65%, put into vapor tight tank after mixing thoroughly, 20 DEG C of lower seals ferment 3 days;
3) preparation of tabasheer ketone: after bamboo fiber being processed the waste water filtering in pretreatment process, with mass fraction be 80% ethanol by volume 1:4 mix, repeat extraction 2 times, Extracting temperature 80 DEG C, extracts 1h at every turn, extract suction filtration, filtrate is concentrated into pulpous state and makes;
4) preparation of wasp dry powder: wasp adult is dried rear pulverizing, crosses 120 mesh sieves and obtains;
5) preparation of nutrient solution: 1.8kg fermented bean dregs is poured into, in 2.3kg syrup, after stirring 20min, pressurization soaks 4h, suction filtration under 20Mpa; Filtrate adds 1.0kg tabasheer ketone, 1.0kg yolk powder, 0.5kg wasp dry powder, the mixing of 0.2kg vitamin, obtained described trichogramma nutrient solution.
By obtained trichogramma nutrient solution in first 1 month of trichogramma egg-laying season feeding trichogramma, its parasitic rate, eclosion rate, single ovum containing honeybee amount, lopsided honeybee rate test result as follows:
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (6)
1. a trichogramma nutrient solution, it is characterized in that: the raw material composition of described trichogramma nutrient solution is counted by weight: syrup 20-23 part, fermented bean dregs 15-18 part, tabasheer ketone 8-10 part, yolk powder 5-10 part, wasp dry powder 3-5 part, B B-complex 1-2 part;
Described syrup is that the honey of nectar source brew is diluted with water obtained by Chinese herbal medicine; Described Chinese herbal medicine comprises honeysuckle, the coptis, Chinese gall, the capsule of weeping forsythia;
Described fermented bean dregs is for major ingredient with dregs of beans, the camellia seed dregs of rice, peanut meal, it is pulverized after 4:3:1 mixing, after adding the corn flour mixing of major ingredient weight 15% ~ 20%, add feed fermentation agent and the water of major ingredient weight 0.01 ~ 0.02% again, material moisture is made to be 60 ~ 65%, vapor tight tank is put into, 20 ~ 25 DEG C of lower seal fermentation 2-3 days after mixing thoroughly.
2. trichogramma nutrient solution according to claim 1, it is characterized in that: described tabasheer ketone is after bamboo fiber being processed the waste water filtering in pretreatment process, with mass fraction be 80% ethanol by volume 2 ~ 4:1 mix, repeat extraction 2 ~ 3 times, Extracting temperature 70 ~ 80 DEG C, each extraction 1 ~ 2h, extract suction filtration, filtrate is concentrated into pulpous state and makes.
3. trichogramma nutrient solution according to claim 1, is characterized in that: described wasp dry powder wasp adult is dried rear pulverizing obtain.
4. a preparation method for trichogramma nutrient solution as claimed in claim 1, is characterized in that: poured into by fermented bean dregs in syrup, and after stirring 20min, under 10-20Mpa, pressurization soaks 4-6h, suction filtration; Filtrate adds tabasheer ketone, yolk powder, wasp dry powder, B B-complex mixing, obtained described trichogramma nutrient solution.
5. an application for trichogramma nutrient solution as claimed in claim 1, is characterized in that: in the trichogramma egg-laying season first 1 month, with described nutrient solution feeding trichogramma; Or set up feeder by 10 ~ 12/mu plantation Tanaka, poured described nutrient solution in feeder confession trichogramma in first 1 month of trichogramma egg-laying season and take food.
6. the application of trichogramma nutrient solution according to claim 5, is characterized in that: described plantation field comprises corn planting field, cotton planting field, Rice Cropping field;
Described feeder is made up of support and drip pan, and its support is formed by connecting by " X " shape mutually by least 3 rods or iron.
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| CN201410500177.8A CN104187206B (en) | 2014-09-26 | 2014-09-26 | A kind of trichogramma nutrient solution and preparation and application thereof |
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Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2064263C1 (en) * | 1992-01-22 | 1996-07-27 | Акционерное общество "Панх" | Corn butterfly control method (variants) |
| RU2063134C1 (en) * | 1992-06-17 | 1996-07-10 | Всероссийский научно-исследовательский институт защиты растений | Trichogram dissemination method for protecting plants against pests |
| JPH06345610A (en) * | 1993-06-15 | 1994-12-20 | Sumitomo Chem Co Ltd | Insecticidal composition containing mummy of trichogramma species |
| US5927004A (en) * | 1996-10-25 | 1999-07-27 | Stocker; Russell | Beneficial insect compositions and method of making same |
| DE102010044601B4 (en) * | 2010-09-07 | 2014-04-30 | BIOCARE Gesellschaft für biologische Schutzmittel mbH | Method for the biological control of pest organisms in a agricultural, horticultural or forestry plant stock |
| CN103859210B (en) * | 2014-02-20 | 2015-10-28 | 广东省昆虫研究所 | A kind of trichogramma synthetic medium |
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