CN104187027A - Megalobrama amblycephala high temperature phase puffed mixed feed and preparation method thereof - Google Patents
Megalobrama amblycephala high temperature phase puffed mixed feed and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a megalobrama amblycephala high temperature phase puffed mixed feed and a preparation method thereof; the megalobrama amblycephala high temperature phase puffed mixed feed comprises the following compositions by weight: 5-15 parts of fish meal, 15-20 parts of soybean meal, 15-20 parts of rapeseed meal, 5-15 parts of cottonseed meal, 15-20 parts of flour, 10-20 parts of rice bran, 0.5-2 parts of monocalcium phosphate, 0.1-0.5 part of a multi premixed material, 0.5-1 part of a multi mineral premix, 0.1-0.5 part of choline chloride, 0.2-0.5 part of salt, 0.5-1.5 parts of soybean oil, 0.01-0.05 part of an antioxidant, 0.1-0.5 parts of an anti mildew agent, 5-10 parts of bentonite, 0.1-0.5 part of coated lysine and 0.1-0.5 parts of powder of radix et rhizoma rhei, radix scutellariae and cortex phellodendri. The megalobrama amblycephala high temperature phase puffed mixed feed can significantly improve the immune ability of megalobrama amblycephala, increase the growth rate of the megalobrama amblycephala, reduces pollution, and is environmentally friendly.
Description
Technical field
The present invention relates to a kind of mixed feed, particularly, relate to a kind of megalobrama amblycephala megathermal period expanding compound feed and preparation method thereof.
Background technology
Megalobrama amblycephala, belongs to Cypriniformes, Cyprinidae, and Culter subfamily, triangular bream belongs to, and is commonly called as blunt snout bream, is one of important phytophage economic fish of China, has the advantages such as feeding habits are wide, survival rate is high, growth is fast, meat is good, delicious flavour because of it, is deeply subject to culturist and consumer's favor.
Southern area of Jiangsu Province is one of megalobrama amblycephala Zhu Yang district of China, and this district is generally used the particulate material megalobrama amblycephala of feeding at present, and the feed drawback of megalobrama amblycephala maximum of particulate material is that residual bait is to severe water pollution.When annual summer high temperature, support family and all can reduce the pollution of residual bait to water quality by the method that reduces the rate of throwing something and feeding day, to reducing the probability of Summer High-Temperature Times megalobrama amblycephala morbidity, although the method can be improved the disease problem of Summer High-Temperature Times megalobrama amblycephala to a certain extent, but the reduction of the rate of throwing something and feeding has inevitably reduced the speed of growth of megalobrama amblycephala, delay the loss of selling fish opportunity and causing raiser's economy.
Therefore, be necessary to provide a set of megalobrama amblycephala megathermal period expanding compound feed formula technique, to ensure that megalobrama amblycephala is in the normal growth speed of megathermal period, and improve its immunocompetence in the megathermal period.
Summary of the invention
The object of this invention is to provide and a kind ofly guarantee megalobrama amblycephala megathermal period normal growth speed and improve special compound feed of its immunocompetence and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of megalobrama amblycephala megathermal period expanding compound feed, comprise the component of following parts by weight: fish meal 5-15 part, dregs of beans 15-20 part, dish dregs of rice 15-20 part, cotton dregs 5-15 part, flour 15-20 part, rice bran 10-20 part, calcium dihydrogen phosphate 0.5-2 part, multidimensional pre-composition 0.1-0.5 part, many ore deposits pre-composition 0.5-1 part, Choline Chloride 0.1-0.5 part, 0.2-0.5 part of salt, soya-bean oil 0.5-1.5 part, antioxidant 0.01-0.05 part, mould inhibitor 0.1-0.5 part, bentonite 5-10 part, coating lysine 0.1-0.5 part, three bloom 0.1-0.5 parts.
In a preferred embodiment, comprise the component of following parts by weight: 10 parts of fish meal, 14 parts of dregs of beans, 23 parts of the dish dregs of rice, 10 parts of cotton dregs, 20 parts, flour, 12 parts, rice bran, 2 parts of calcium dihydrogen phosphates, 0.1 part of multidimensional pre-composition, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 2 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 4.93 parts of bentonites, 0.5 part of coating lysine, three 0.5 part of bloom.
In another preferred embodiment, comprise the component of following parts by weight: 12 parts of fish meal, 16 parts of dregs of beans, 14 parts of the dish dregs of rice, 15 parts of cotton dregs, 18 parts, flour, 14 parts, rice bran, 1 part of calcium dihydrogen phosphate, 0.1 part of multidimensional pre-composition, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 2 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 6.53 parts of bentonites, 0.1 part of coating lysine, three 0.3 part of bloom.
In another preferred embodiment, comprise the component of following parts by weight: 14 parts of fish meal, 13 parts of dregs of beans, 17 parts of the dish dregs of rice, 13 parts of cotton dregs, 19 parts, flour, 13 parts, rice bran, 0.1 part of multidimensional pre-composition, 1.5 parts of calcium dihydrogen phosphates, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 1.5 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 6.33 parts of bentonites, 0.2 part of coating lysine, three 0.4 part of bloom.
Prepare a method for above-mentioned megalobrama amblycephala megathermal period expanding compound feed, after above-mentioned raw materials is pulverized, mix according to the above ratio, after separating twice, can be made into through fodder machine.
Compared with commercially available normal diet, the present invention has following advantage: 1, megalobrama amblycephala megathermal period expanding compound feed can reduce feed waste to greatest extent, reduces the pollution of residual bait to water quality; 2, megalobrama amblycephala uses after this feed, and the speed of growth is better than commercially available plain particles feed; 3, megalobrama amblycephala is ingested after this feed, and immunocompetence improves.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is done to detailed description further, but embodiments of the present invention are not limited to this.
Embodiment 1
Get the feedstuff after pulverizing: 10 parts of fish meal, 14 parts of dregs of beans, 23 parts of the dish dregs of rice, 10 parts of cotton dregs, 20 parts, flour, 12 parts, rice bran, 2 parts of calcium dihydrogen phosphates, 0.1 part of multidimensional pre-composition, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 2 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 4.93 parts of bentonites, 0.5 part of coating lysine, three 0.5 part of bloom.After fully being mixed, above-mentioned raw materials through separating twice, makes megalobrama amblycephala megathermal period expanding compound feed with fodder machine.
The forage protein content that the present embodiment is made is 31.5%, and fat content reaches 5%, and lysine content reaches 1.9%, can fully meet the nutritional need of megalobrama amblycephala.The feed providing with this example carries out culture experiment to megalobrama amblycephala (initial specification is 200g), adopt commercially available megalobrama amblycephala feed in contrast, 5 repetitions are established in each processing, experiment is carried out in net cage, culture-cycle is 1 month, in experimentation, water temperature excursion is 32-36 DEG C, being satiated with food every day twice, the feed of experiment (at 9 in the morning and afternoons 3 point) of throwing something and feeding.Experimental result is as follows:
Wherein, the formula of commercially available megalobrama amblycephala feed is: 4 parts of fish meal, 15 parts of dregs of beans, 25 parts of the dish dregs of rice, 20 parts of cotton dregs, 10 parts, rice bran, 18 parts, flour, 1 part of calcium dihydrogen phosphate, 0.1 part of multidimensional pre-composition, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 2 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 3.93 parts of bentonites.
Experiment shows, uses the megalobrama amblycephala speed of growth of the megalobrama amblycephala megathermal period expanded pellet diet cultivation that this example provides to be obviously better than plain particles megalobrama amblycephala feed.
Embodiment 2
Get the feedstuff after pulverizing: 12 parts of fish meal, 16 parts of dregs of beans, 14 parts of the dish dregs of rice, 15 parts of cotton dregs, 18 parts, flour, 14 parts, rice bran, 1 part of calcium dihydrogen phosphate, 0.1 part of multidimensional pre-composition, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 2 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 6.53 parts of bentonites, 0.1 part of coating lysine, three 0.3 part of bloom.After fully being mixed, above-mentioned raw materials through separating twice, makes megalobrama amblycephala megathermal period expanding compound feed with fodder machine.
The forage protein content that the present embodiment is made is 31.5%, and fat content reaches 5%, and lysine content reaches 1.9%, can fully meet the nutritional need of megalobrama amblycephala.
The feed providing with this example and common commercial megalobrama amblycephala feed carry out culture experiment to megalobrama amblycephala, experiment is carried out in pond, culture-cycle is 1 month, and in experimentation, water temperature excursion is 32-36 DEG C, twice, the feed of experiment of throwing something and feeding every day (at 9 in the morning and afternoons 3 point).After experiment finishes, with tail fin blood sampling, make blunt-snout gurnards serum.Detect lysozyme content in blunt-snout gurnards serum taking micrococcus lysodeikticus freeze-dried powder (Sigma company product) as substrate, follow these steps to carry out: join substrate suspension (OD570nm=0.13～0.15) with the phosphate buffer of 1/15 mol/L pH 6.9.Get 3 mL suspensions in vitro, put in ice bath, add 50 μ L serum and mix, survey its light absorption value A0 at 570 nm places.Then test tube is moved into 30 min in 25 DEG C of water-baths, after taking-up, be placed in immediately ice bath 10 min with cessation reaction, survey the light absorption value A at its 570 nm place.Press the activity of formula calculating lysozyme: U=(A0-A)/A0, experimental result is as follows:
Wherein, the formula of commercially available megalobrama amblycephala feed is: 4 parts of fish meal, 18 parts of dregs of beans, 23 parts of the dish dregs of rice, 17 parts of cotton dregs, 12 parts, rice bran, 18 parts, flour, 1 part of calcium dihydrogen phosphate, 0.1 part of multidimensional pre-composition, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 2 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 3.93 parts of bentonites.
Experiment shows, megalobrama amblycephala megathermal period expanded pellet diet provided by the invention can significantly improve the serum lysozyme level of megathermal period megalobrama amblycephala, thereby improves its immunocompetence.
Embodiment 3
Get the feedstuff after pulverizing: 14 parts of fish meal, 13 parts of dregs of beans, 17 parts of the dish dregs of rice, 13 parts of cotton dregs, 19 parts, flour, 13 parts, rice bran, 0.1 part of multidimensional pre-composition, 1.5 parts of calcium dihydrogen phosphates, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 1.5 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 6.33 parts of bentonites, 0.2 part of coating lysine, three 0.4 part of bloom.After fully being mixed, above-mentioned raw materials through separating twice, makes megalobrama amblycephala megathermal period expanding compound feed with fodder machine.
The forage protein content 32.0% that the present embodiment is made, fat content reaches 5%, and lysine content reaches 1.9%, can fully meet the nutritional need of megalobrama amblycephala.
The feed providing with this example and general commercial feed carry out culture experiment to megalobrama amblycephala respectively, experiment is carried out in pond, culture-cycle is 1 month, and in experimentation, water temperature excursion is 32-36 DEG C, twice, the feed of experiment of throwing something and feeding every day (at 9 in the morning and afternoons 3 point).After experiment finishes, detection head nephrocyte phagocytic activity and serum lysozyme level.Phagocytic rate assay method is: take out megalobrama amblycephala head-kidney, at L-15 nutrient solution (2% calf serum, 1% penicillin/streptomycin, 0.2% heparin) in gently comb spread to cell suspension, after 120 order metal mesh filters, cell is added in 34-51%Percoll gradient to the centrifugal 30min of 400g, collection is positioned at 34% and the cell of 51%Percoll interface, with L-15 nutrient solution just cell density be adjusted to 106/ml.Get 108/ml brewer's yeast suspension and close the each 0.1ml of renal cell suspension preparing, after fully mixing, making humidity strip, after 0.25% glutaraldehyde is fixing, with the purple dyeing microscopic examination of first class, adding up the cell number of engulfing brewer's yeast in 200 renal cells.
Phagocytic rate (%)=(engulfing renal cell number/200 of yeast) × 100
Serum lysozyme sharp detection method alive is as follows: tail vein blood, makes blunt-snout gurnards serum.Detect lysozyme content in blunt-snout gurnards serum taking micrococcus lysodeikticus freeze-dried powder (Sigma company product) as substrate, follow these steps to carry out: join substrate suspension (OD570nm=013～015) with the phosphate buffer of 1/15 mol/L pH 6.9.Get 3 mL suspensions in vitro, put in ice bath, add 50 μ L serum and mix, survey its light absorption value A0 at 570 nm places.Then test tube is moved into 30 min in 25 DEG C of water-baths, after taking-up, be placed in immediately ice bath 10 min with cessation reaction, survey the light absorption value A at its 570 nm place.Press the activity of formula calculating lysozyme: U=(A0-A)/A0.Experimental result is as follows:
Wherein, the formula of described general commercial feed is: 4.5 parts of fish meal, 20 parts of dregs of beans, 21 parts of the dish dregs of rice, 14 parts of cotton dregs, 15 parts, rice bran, 17 parts, flour, 1 part of calcium dihydrogen phosphate, 0.1 part of multidimensional pre-composition, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 2 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 4.43 parts of bentonites.
Experiment shows, applies feed provided by the invention and raises megalobrama amblycephala in the megathermal period, and its immunocompetence is significantly better than general commercial feed group.Especially, the phagocytic rate of the megalobrama amblycephala of the forage feed described in the present embodiment and antalzyme activity are the more than 3 times of megalobrama amblycephala of general commercial forage feed, possess notable difference (P < 0.05).
As mentioned above, can realize preferably the present invention.
The above; it is only preferred embodiment of the present invention; not the present invention is done to any pro forma restriction; according to technical spirit of the present invention; within the spirit and principles in the present invention; any simple amendment that above embodiment is done, be equal to and replace and improve etc., within all still belonging to the protection domain of technical solution of the present invention.
Claims (5)
1. a megalobrama amblycephala megathermal period expanding compound feed, it is characterized in that, comprise the component of following parts by weight: fish meal 5-15 part, dregs of beans 15-20 part, dish dregs of rice 15-20 part, cotton dregs 5-15 part, flour 15-20 part, rice bran 10-20 part, calcium dihydrogen phosphate 0.5-2 part, multidimensional pre-composition 0.1-0.5 part, many ore deposits pre-composition 0.5-1 part, Choline Chloride 0.1-0.5 part, 0.2-0.5 part of salt, soya-bean oil 0.5-1.5 part, antioxidant 0.01-0.05 part, mould inhibitor 0.1-0.5 part, bentonite 5-10 part, coating lysine 0.1-0.5 part, three bloom 0.1-0.5 parts.
2. a megalobrama amblycephala megathermal period expanding compound feed, is characterized in that, comprises the component of following parts by weight: 10 parts of fish meal, 14 parts of dregs of beans, 23 parts of the dish dregs of rice, 10 parts of cotton dregs, 20 parts, flour, 12 parts, rice bran, 2 parts of calcium dihydrogen phosphates, 0.1 part of multidimensional pre-composition, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 2 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 4.93 parts of bentonites, 0.5 part of coating lysine, three 0.5 part of bloom.
3. a megalobrama amblycephala megathermal period expanding compound feed, is characterized in that, comprises the component of following parts by weight: 12 parts of fish meal, 16 parts of dregs of beans, 14 parts of the dish dregs of rice, 15 parts of cotton dregs, 18 parts, flour, 14 parts, rice bran, 1 part of calcium dihydrogen phosphate, 0.1 part of multidimensional pre-composition, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 2 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 6.53 parts of bentonites, 0.1 part of coating lysine, three 0.3 part of bloom.
4. a megalobrama amblycephala megathermal period expanding compound feed, is characterized in that, comprises the component of following parts by weight: 14 parts of fish meal, 13 parts of dregs of beans, 17 parts of the dish dregs of rice, 13 parts of cotton dregs, 19 parts, flour, 13 parts, rice bran, 0.1 part of multidimensional pre-composition, 1.5 parts of calcium dihydrogen phosphates, 0.5 part of many ore deposits pre-composition, 0.15 part of Choline Chloride, 0.2 part of salt, 1.5 parts, soya-bean oil, 0.02 part of antioxidant, 0.1 part of mould inhibitor, 6.33 parts of bentonites, 0.2 part of coating lysine, three 0.4 part of bloom.
5. a method of preparing the megalobrama amblycephala megathermal period expanding compound feed as described in any one in claim 1 to 4, is characterized in that, comprises the following steps: after above-mentioned raw materials is pulverized, mix according to the above ratio, after separating twice, can be made into through fodder machine.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104543562A (en) * | 2015-01-08 | 2015-04-29 | 华中农业大学 | Feed capable of improving highly unsaturated fatty acids of megalobrama amblycephala muscles, and preparation method and application |
| CN104905072A (en) * | 2015-06-30 | 2015-09-16 | 南通巴大饲料有限公司 | Compound feed for megalobrama amblycephala and preparation method of compound feed |
| CN104938804A (en) * | 2015-05-18 | 2015-09-30 | 安徽天助饲料有限公司 | Special compound feed for mixed-culture fish and preparation method of special compound feed |
| CN110150503A (en) * | 2018-03-14 | 2019-08-23 | 湖南正虹科技发展股份有限公司 | A kind of mixed feed and preparation method thereof restoring megalobrama amblycephala epidermal mucus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104543562A (en) * | 2015-01-08 | 2015-04-29 | 华中农业大学 | Feed capable of improving highly unsaturated fatty acids of megalobrama amblycephala muscles, and preparation method and application |
| CN104543562B (en) * | 2015-01-08 | 2017-06-09 | 华中农业大学 | A kind of feed and preparation method and application for improving megalobrama amblycephala muscle highly unsaturated fatty acid |
| CN104938804A (en) * | 2015-05-18 | 2015-09-30 | 安徽天助饲料有限公司 | Special compound feed for mixed-culture fish and preparation method of special compound feed |
| CN104905072A (en) * | 2015-06-30 | 2015-09-16 | 南通巴大饲料有限公司 | Compound feed for megalobrama amblycephala and preparation method of compound feed |
| CN110150503A (en) * | 2018-03-14 | 2019-08-23 | 湖南正虹科技发展股份有限公司 | A kind of mixed feed and preparation method thereof restoring megalobrama amblycephala epidermal mucus |
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