CN104170741A - Culture medium for preserving chrysanthemum tissue culture seedlings - Google Patents
Culture medium for preserving chrysanthemum tissue culture seedlings Download PDFInfo
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- CN104170741A CN104170741A CN201410452417.1A CN201410452417A CN104170741A CN 104170741 A CN104170741 A CN 104170741A CN 201410452417 A CN201410452417 A CN 201410452417A CN 104170741 A CN104170741 A CN 104170741A
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- chrysanthemum
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- nutrient solution
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Abstract
The invention discloses a culture medium for preserving chrysanthemum tissue culture seedlings. The culture medium is characterized by using a peat and perlite mixed matrix instead of a traditional agar matrix and adding a nutrient solution containing various nutrient substances and plant hormones to the peat and perlite mixed matrix. Growth of chrysanthemum can be greatly slowed by culturing chrysanthemum on the culture medium, thus achieving the aim of preserving the chrysanthemum tissue culture seedlings for a long time. Compared with the traditional plant tissue culture seedling preservation technology, the culture medium has the advantages that the culture medium is used for culturing chrysanthemum by adopting normal temperature conditions for chrysanthemum tissue culture, is low in expense and cost and has good effects.
Description
Technical field
a Storaged media for chrysanthemum group training seedling, relates to chrysanthemum tissue culture technique field, says more specifically a kind of chrysanthemum group training seedling is carried out to the long-term medium of preserving.
Background technology
it is the important means of carrying out the productions such as chrysanthemum Fast-propagation, genetic mutagenesis and research work that the tissue of chrysanthemum is cultivated.But because fast numerous production and scientific research have certain stage, inevitably have the lag phase of certain hour therebetween.For example production unit has completed according to client's needs the seedling amount that order needs, and new order also cannot be expected; Or R&D institution completed or come to an end the research of chrysanthemum aspect, within the regular hour, needn't breed again chrysanthemum seedling.Now the culture units of organizing of chrysanthemum will face the problem that group training seedling is preserved: how to allow the chrysanthemum seedling of group training grow lentamente, by growing slowly, the growth time of prolongation group training seedling in bottle, reduces the operation of subculture, has both saved and has manually also saved material.Because the group of chrysanthemum is trained the fast especially of seedling growth, the seedling of generally taking root just can cover with one bottle about 30 days, if do not carry out subculture cultivation, seedling body will be aging gradually, finally dead, made group training material loss.Now, if there is market demand, transform cultivation possibility from explant the aid is too slow to be helpful temporarily, misses market good opportunity.If but chrysanthemum seedling is carried out to succeeding preservation, and will increase the consumption of human and material resources, cause waste.So invent, a kind of can to organize the technology of preserving chrysanthemum tissue culturing seedling under cultivation temperature condition at normal chrysanthemum be that industry is expected.
Summary of the invention
the object of the present invention is to provide a kind of medium that carries out the preservation of chrysanthemum group training seedling under cultivation temperature of organizing at normal chrysanthemum, preserve to reduce chrysanthemum group training seedling the running cost of cultivating.
the present invention is achieved in that
1. a Storaged media for chrysanthemum group training seedling, its formula is:
(1) matrix: matrix adopts the mixture of the peat composed of rotten mosses and perlite 1:1;
(2) nutrient solution: in nutrient solution, the concentration of macroelement, trace element, organic principle and molysite, all according to the requirement preparation of 1/2MS medium, separately adds 0.2mg/L NAA, 0.2mg/L BA and 20g/L sucrose;
(3) consumption: by the tissue culture flasks of 250ml, every bottle adds mixed-matrix 50ml, nutrient solution 40ml.
operating procedure:
(1) preparation of matrix: the peat composed of rotten mosses is put together and mixed according to the weight portion of 1:1 with perlite;
(2) preparation nutrient solution;
(3) bottling: add matrix and nutrient solution in tissue culture bottle;
(4) sterilizing: carry out sterilizing with autoclave sterilizer;
(5) inoculation: treat after sterilizing that tissue culture bottle is cooling, inoculate in matrix, every bottle graft enters 1-5 single stem segment (being preferably 1);
(6) cultivate: tissue culture bottle is put into group training chamber and cultivate according to normal chrysanthemum conditions of tissue culture: 25 ± 1 DEG C of temperature, the about 1500lx(light source of intensity of illumination is 40 W fluorescent lamps), light application time 12 h/d.
beneficial effect of the present invention:
adopt Storaged media of the present invention to preserve the group training seedling of chrysanthemum, can organize under cultivation temperature at normal chrysanthemum, the holding time of the training of prolongation group greatly seedling, labor and material saving, reduces expenses.In the time there is the fast numerous demand of chrysanthemum, can from Storaged media, take out immediately chrysanthemum seedling, get back to and in agar medium, carry out fast numerous cultivation; If there is no fast numerous demand, unit can utilize medium of the present invention to carry out the long-term preservation of material, and growth cycle is long, and subculture is long interval time.The application of basal culture medium can improve the strategic maneuver of relevant enterprise and R&D institution, reduces expenditure and increases income.
embodiment:
1. the formula of the Storaged media of a chrysanthemum group training seedling:
(1) matrix: matrix adopts the mixture of the peat composed of rotten mosses and perlite 1:1;
(2) nutrient solution: in nutrient solution, the concentration of macroelement, trace element, organic principle and molysite, all according to the requirement preparation of 1/2MS medium, separately adds 0.2mg/L NAA, 0.2mg/L BA and 20g/L sucrose;
(3) consumption: by the tissue culture flasks of 250ml, every bottle adds mixed-matrix 50ml, nutrient solution 40ml.
operating procedure:
(1) preparation of matrix: the peat composed of rotten mosses is put together and mixed according to the weight portion of 1:1 with perlite;
(2) preparation nutrient solution;
(3) bottling: add matrix and nutrient solution in tissue culture bottle;
(4) sterilizing: carry out sterilizing with autoclave sterilizer;
(5) inoculation: treat after sterilizing that tissue culture bottle is cooling, inoculate in matrix, every bottle graft enters 1 single stem segment, adds a cover;
(6) cultivate: tissue culture bottle is put into group training chamber and cultivate according to normal chrysanthemum conditions of tissue culture: 25 ± 1 DEG C of temperature, the about 1500lx(light source of intensity of illumination is 40 W fluorescent lamps), light application time 12 h/d.
test situation:
tested on September 1st, 2013 and carry out in Anhui Science and Technology College's Plant Tissue Breeding laboratory.According to single stem segment of above-mentioned formula and operating procedure inoculation chrysanthemum group training seedling, taking the agar medium medium of 1/2MS as contrast; 20 bottles of every processing inoculations, every bottle graft kind 5 strains.
growth investigation: as seen from Table 1, in Storaged media of the present invention, the tissue culturing seedling growth of chrysanthemum is obviously slack-off, from be inoculated into cover with bottle and from time of being inoculated into the death of half seedling all obviously contrast greatly increase, effectively extended the holding time of chrysanthemum group training seedling.
two kinds of medium chrysanthemum group training seedling growing state tables of table 1.
Claims (1)
1. a Storaged media for chrysanthemum group training seedling, is characterized in that, its formula is as follows:
(1) matrix: matrix is the mixture of the peat composed of rotten mosses and perlite 1:1;
(2) nutrient solution: in nutrient solution, the concentration of macroelement, trace element, organic principle and molysite, all according to the requirement preparation of 1/2MS medium, separately adds 0.2mg/L NAA, 0.2mg/L BA and 30g/L sucrose;
(3) consumption: by the tissue culture flasks of 250ml, every bottle adds mixed-matrix 50ml, nutrient solution 40ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410452417.1A CN104170741B (en) | 2014-09-09 | 2014-09-09 | A kind of Storaged media of chrysanthemum tissue cultured seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410452417.1A CN104170741B (en) | 2014-09-09 | 2014-09-09 | A kind of Storaged media of chrysanthemum tissue cultured seedling |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104170741A true CN104170741A (en) | 2014-12-03 |
| CN104170741B CN104170741B (en) | 2016-04-13 |
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| CN201410452417.1A Expired - Fee Related CN104170741B (en) | 2014-09-09 | 2014-09-09 | A kind of Storaged media of chrysanthemum tissue cultured seedling |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106416981A (en) * | 2016-10-14 | 2017-02-22 | 泉州市泉美生物科技有限公司 | Method for planting plants in transparent sealed container and applied substrate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015274A (en) * | 2007-02-16 | 2007-08-15 | 北京林业大学 | Method for breeding novel varieties of ground-cover chrysanthemum utilizing electron beam radiation combined with tissue culture |
| CN101215550A (en) * | 2008-01-16 | 2008-07-09 | 浙江省农业科学院 | Tissue culture bulb preserving method for thunberg fritillary virus |
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2014
- 2014-09-09 CN CN201410452417.1A patent/CN104170741B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015274A (en) * | 2007-02-16 | 2007-08-15 | 北京林业大学 | Method for breeding novel varieties of ground-cover chrysanthemum utilizing electron beam radiation combined with tissue culture |
| CN101215550A (en) * | 2008-01-16 | 2008-07-09 | 浙江省农业科学院 | Tissue culture bulb preserving method for thunberg fritillary virus |
Non-Patent Citations (1)
| Title |
|---|
| 吴仕超: "42份菊科花卉的离体保存及其遗传多样性的RAPD分析", 《福建农林大学硕士学位论文》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106416981A (en) * | 2016-10-14 | 2017-02-22 | 泉州市泉美生物科技有限公司 | Method for planting plants in transparent sealed container and applied substrate |
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| CN104170741B (en) | 2016-04-13 |
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Inventor after: Zhang Xueping Inventor after: Lin Ping Inventor before: Lin Ping Inventor before: He Keqin Inventor before: Li Jie |
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