CN104147644A - Decalcified dental matrix of mammal and preparation method thereof - Google Patents
Decalcified dental matrix of mammal and preparation method thereof Download PDFInfo
- Publication number
- CN104147644A CN104147644A CN201310602878.8A CN201310602878A CN104147644A CN 104147644 A CN104147644 A CN 104147644A CN 201310602878 A CN201310602878 A CN 201310602878A CN 104147644 A CN104147644 A CN 104147644A
- Authority
- CN
- China
- Prior art keywords
- mammal
- decalcification
- bubble
- teeth
- minutes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention relates to a decalcified dental matrix of a mammal and a preparation method thereof. The raw material is from permanent teeth of the mammal. The preparation method comprises the following steps: (1) collecting permanent teeth of the mammal for later use; (2) soaking the permanent teeth in a 50% 84 disinfectant for sterilizing, wherein the time for sterilizing is 45 minutes and the temperature is 35-40DEG C; (3) removing useless parts of the teeth; (4) smashing tooth tissues into particles; (5) sterilizing the smashed tooth tissue particles, wherein the time for sterilizing is 45 minutes and the temperature is 35-40DEG C; (6) putting the treated tooth tissue particles into 0.65-0.72 equivalent weight of hydrochloric acid liquid for soaking for 10-16 hours at 34-38DEG C to finish decalcification; and (7) drying at low temperature, soaking in hydrogen peroxide for 30 minutes, so as to obtain the decalcified dental matrix of the mammal. The decalcified dental matrix of the mammal has better inductive bone regenerative action, and does not carry any pathogenic factor after being prepared into final products.
Description
Technical field
The present invention relates to a kind of medical material and preparation method thereof, particularly relate to a kind of decalcification mammal teeth matrix and preparation method thereof.
Background technology
Propose bone induction the U.S. one scholar theoretical, think that it has the function of bringing out body osteanagenesis to have a kind of bone morphogenetic protein (BMP) in animals and humans sclerous tissues (bone and tooth).The sclerous tissues that selects fresh, healthy animals or humans is raw material, remove soft tissue by machining, be ground into granule, after washing, pass through again chemical process degrease, then sclerous tissues's fragment is inserted in 0.6 equivalent hydrochloric acid solution, sclerous tissues's substrate is produced in constant temperature decalcification.This type of sclerous tissues's substrate is being implanted into after body, wherein contained bone morphogenetic protein can produce directional induction in implantation region, make original Interstitial cell that directed merisis skeletonization or chondrocyte occur, thereby make osteanagenesis, reach the object that bone is rebuild.Medically adopting discarded people's tooth as raw material as the replacement material on the skeletons such as people's dental repair wound wound has had at present, but people's tooth source is very limited, unappeasable needs far away; So still with synthetic materials such as steel plates, but steel plate and synthetic material are difficult to and bone merges, and more can not make osteanagenesis.So searching and discovery are more effective, are more value in use new bone induction material, are biomaterial for medical purpose research field important topics always.Bone morphogenetic protein (BMP) is extensively present in mammiferous sclerous tissues, has been extensively to know together in the world.Wherein the highest with dentin and cortical bone content.Cannot thoroughly remove because of osteocyte composition contained in cortical bone, the exogenous paathogenic factor of this series products can not be solved, use security hidden trouble and produce.Because cannot thoroughly removing, osteocyte composition make rejection obvious simultaneously.And dentin is unique sclerous tissues of not containing cell, thus taking tooth as raw material make bone induction material have a clear superiority in compared with osseous tissue.
But, making a general survey of more than 30 year domestic and international research process and find, why utilizing tooth there is no large progress aspect raw material making bone induction material, is because everybody is selecting to make mistakes on raw material tooth always, the use of mistake deciduous teeth be raw material, so just can not obtain valuable experimental result.I find through long-term experiment research, mammal comprise the mankind be born after two cover tooth, early stage deciduous teeth and later permanent teeths all.The dentin of deciduous teeth is few undesirable for making bone induction material effect, and permanent teeth is ideal with regard to complete different making bone induction material effect.And mammal growth course is to allow people control completely, can obtain maximum safety guarantee and supply obtaining on raw material tooth.
Summary of the invention
The object of this invention is to provide a kind of decalcification mammal teeth matrix that a kind of raw material sources are wide, with low cost, technique is simple, serviceability is good and preparation method thereof.
The object of the present invention is achieved like this:
A kind of decalcification mammal teeth matrix. selected raw material is that mammiferous permanent teeth is raw material.
A kind of manufacture method of decalcification mammal teeth matrix.
A manufacture method for decalcification mammal teeth matrix, comprises following making step:
(1) first by for subsequent use the mammal permanent teeth cryopreservation of collecting;
(2) raw material tooth is invaded bubble sterilization with 50% 84 disinfectant solution, and it is that 45 minutes, temperature are 35-40 Celsius that 84 disinfectant solution are invaded the bubble time;
(3) remove with mechanical means the part having no value for use on tooth body;
(4) by the method for mechanical activation comminution, dental tissue is ground into granule;
(5) invade bubble disinfectant powder with 50% 84 disinfectant solution again and be broken into granule dental tissue, it is that 45 minutes, temperature are 35-40 Celsius that 84 disinfectant solution are invaded the bubble time;
(6) the dental tissue granule of processing is placed to 0.65-0.72 equivalent hydrochloric acid solution and invades bubble, under 34-38 degree Celsius, invade bubble and within 10-16 hour, complete decalcification;
(7) again by dentin cold drying after decalcification, then with 3% dioxygen water enchroachment (invasion) bubble, temperature 35 degree Celsius of dioxygen water enchroachment (invasion) bubble, 30 minutes time, obtain required decalcification mammal teeth matrix.
The mammal teeth matrix that above-mentioned said decalcification technique obtains, under the condition of 5 DEG C of Celsius temperatures, insert in 70% ethanol liquid, store for subsequent use.
Above-mentioned said mammal comprises cattle, sheep, pig etc.
84 described disinfectant solution are a kind of taking sodium hypochlorite as main high effect disinfectants, and main component is sodium hypochlorite (NaClO).Colourless or weak yellow liquid, available chlorine content 5.5～6.5%.Be widely used in the disinfecting of hotel, tourism, hospital, food-processing industry, family etc.
Beneficial effect of the present invention: the teeth matrix of the decalcification mammal that employing this method obtains, has good inductive bone regeneration effect.Detect and show by bio-safety, invade the raw material of bubble sterilization through dual 50%84 disinfectant solution, after formation final products, can not carry again any virulence factor (comprising antibacterial, virus, particularly haematogenous infection virus and nucleic acid that may be residual).Through cold drying, entrained hydrogen peroxide in decalcification teeth matrix hole after 3% dioxygen water enchroachment (invasion) bubble can be subject to implantation region to discharge fast oxonium ion and be combined with hemocyte and form fibrous reticular structure in human body.This cancellated formation can effectively improve material and stop stability in implantation region, also can improve the growth activity of implantation region simultaneously.This decalcification mammal teeth matrix can be used for that the bone that can not heal voluntarily that causes because of various physics and chemistry, pathology reason for the treatment of is damaged, bone structure reconstruction, and mixes use with artificial bone, to strengthen artificial bone's the bone grafting such as growth fastness; The tooth raw material sources of mammal are easy, because material abundance can industrialization can meet medical required.
Detailed description of the invention
Embodiment 1:
A kind of decalcification mammal teeth matrix. selected raw material is that the permanent teeth of mammal cattle is raw material.
A manufacture method for decalcification mammal teeth matrix, comprises following making step:
(1) first by for subsequent use the mammal cattle permanent teeth cryopreservation of collecting;
(2) raw material tooth is invaded bubble sterilization with 50% 84 disinfectant solution, and it is that 45 minutes, temperature are 35-40 Celsius that 84 disinfectant solution are invaded the bubble time;
(3) remove with mechanical means the part having no value for use on tooth body;
(4) by the method for mechanical activation comminution, dental tissue is ground into granule;
(5) invade bubble disinfectant powder with 50% 84 disinfectant solution again and be broken into granule dental tissue, it is that 45 minutes, temperature are 35-40 Celsius that 84 disinfectant solution are invaded the bubble time;
(6) the dental tissue granule of processing is placed to 0.65-0.72 equivalent hydrochloric acid solution and invades bubble, under 34-38 degree Celsius, invade bubble and within 10-16 hour, complete decalcification;
(7) again by dentin cold drying after decalcification, then with 3% dioxygen water enchroachment (invasion) bubble, temperature 35 degree Celsius of dioxygen water enchroachment (invasion) bubble, 30 minutes time, obtain required decalcification mammal teeth matrix.
The mammal teeth matrix that above-mentioned said decalcification technique obtains, under the condition of 5 DEG C of Celsius temperatures, insert in 70% ethanol liquid, store for subsequent use.
Embodiment 2:
A kind of decalcification mammal teeth matrix. selected raw material is that the permanent teeth of mammal sheep is raw material.
A manufacture method for decalcification mammal teeth matrix, comprises following making step:
(1) first by for subsequent use the mammal permanent teeth cryopreservation of collecting;
(2) 50% 84 disinfectant solution for raw material tooth, the best is invaded 45 minutes bubble time, optimum temperature 36 degree Celsius;
(3) remove with mechanical means the part having no value for use on tooth body;
(4) by the method for mechanical activation comminution, dental tissue is ground into granule;
(5) invade bubble disinfectant powder with 50% 84 disinfectant solution again and be broken into granule dental tissue, 84 disinfectant solution the bests are invaded 45 minutes bubble time, optimum temperature 36 degree Celsius;
(6) the dental tissue granule of processing is placed to 0.65-0.72 equivalent hydrochloric acid solution and invades bubble, best hydrochloric acid solution concentration 0.68 equivalent, optimum temperature is at 35 degrees Celsius,, the best is invaded 14 hours bubble time.
(7) again by dentin cold drying after decalcification, then with 3% dioxygen water enchroachment (invasion) bubble, temperature 35 degree Celsius of dioxygen water enchroachment (invasion) bubble, 30 minutes time, obtain required decalcification mammal teeth matrix.
The mammal teeth matrix that above-mentioned said decalcification technique obtains, under the condition of 5 DEG C of Celsius temperatures, insert in 70% ethanol liquid, store for subsequent use.
Clinical trial case
Case 1: patient: female, 48 years old.Lower front dental bed repeatedly pyorrhea accompanies odontoseisis more than 1 year, the multiple fistulas of 2-2 lip side before health check-up discovery is lower, and become flexible with tooth 2-2 degree; X-ray prompting 2-2 apical area has multiple diameters at 6mm printing opacity shade, the lower multiple radicular cyst of labial teeth of diagnosis, and treatment 2-2 root canal completes; 2-2 operation root resection again, cyst is struck off; 100mg baurodont host material is implanted respectively in tip of a root bone defective region, and postoperative X-ray prompting in January bone defective region new bone growth is good.
Case 2: patient: man, 60 years old.Upper left labial teeth often swells and ache for many years, health check-up upper left jaw 1-2 tooth lip side fistula, and swelling and aching of gum, X-ray prompting upper left jaw 1-2 tooth has 2cm printing opacity shade, and treatment 1-2 root canal treatment completes, then 1-2 tooth operation root resection, and cyst is struck off; 1g baurodont host material is implanted respectively in tip of a root bone defective region; Postoperative X-ray prompting in January bone defective region new bone growth is good.
Claims (4)
1. a decalcification mammal teeth matrix. it is characterized in that: selected raw material is that mammiferous permanent teeth is raw material.
2. a manufacture method for decalcification mammal teeth matrix, is characterized in that comprising following making step:
(1) first by for subsequent use the mammal permanent teeth cryopreservation of collecting;
(2) raw material tooth is invaded bubble sterilization with 50% 84 disinfectant solution, and it is that 45 minutes, temperature are 35-40 Celsius that 84 disinfectant solution are invaded the bubble time;
(3) remove with mechanical means the part having no value for use on tooth body;
(4) by the method for mechanical activation comminution, dental tissue is ground into granule;
(5) invade bubble disinfectant powder with 50% 84 disinfectant solution again and be broken into granule dental tissue, it is that 45 minutes, temperature are 35-40 Celsius that 84 disinfectant solution are invaded the bubble time;
(6) the dental tissue granule of processing is placed to 0.65-0.72 equivalent hydrochloric acid solution and invades bubble, under 34-38 degree Celsius, invade bubble and within 10-16 hour, complete decalcification;
(7) again by dentin cold drying after decalcification, then with 3% dioxygen water enchroachment (invasion) bubble, temperature 35 degree Celsius of dioxygen water enchroachment (invasion) bubble, 30 minutes time, obtain required decalcification mammal teeth matrix.
3. the manufacture method of a kind of decalcification mammal teeth matrix according to claim 2, is characterized in that: the mammal teeth matrix that above-mentioned said decalcification technique obtains, under the condition of 5 DEG C of Celsius temperatures, insert in 70% ethanol liquid, store for subsequent use.
4. the manufacture method of a kind of decalcification mammal teeth matrix according to claim 2, is characterized in that: above-mentioned said mammal comprises cattle, sheep, pig etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310602878.8A CN104147644A (en) | 2013-11-26 | 2013-11-26 | Decalcified dental matrix of mammal and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310602878.8A CN104147644A (en) | 2013-11-26 | 2013-11-26 | Decalcified dental matrix of mammal and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104147644A true CN104147644A (en) | 2014-11-19 |
Family
ID=51873403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310602878.8A Pending CN104147644A (en) | 2013-11-26 | 2013-11-26 | Decalcified dental matrix of mammal and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104147644A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106823001A (en) * | 2017-04-12 | 2017-06-13 | 吉林大学 | A kind of biologic bracket material, preparation method and applications for dental root regeneration |
| CN107496988A (en) * | 2017-07-20 | 2017-12-22 | 深圳市光明创博生物制品发展有限公司 | A kind of lyophilized dentale powder material and preparation method thereof |
| CN110732040A (en) * | 2018-07-19 | 2020-01-31 | 上海交通大学医学院附属第九人民医院 | bone repair material, method and use |
| CN114795556A (en) * | 2022-05-10 | 2022-07-29 | 图斯邦柯生物科技(苏州)有限公司 | In vitro tooth bone storage and preparation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1116121A (en) * | 1995-08-15 | 1996-02-07 | 李成林 | Decalcifying tooth matrix and making method |
-
2013
- 2013-11-26 CN CN201310602878.8A patent/CN104147644A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1116121A (en) * | 1995-08-15 | 1996-02-07 | 李成林 | Decalcifying tooth matrix and making method |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106823001A (en) * | 2017-04-12 | 2017-06-13 | 吉林大学 | A kind of biologic bracket material, preparation method and applications for dental root regeneration |
| CN106823001B (en) * | 2017-04-12 | 2019-11-05 | 吉林大学 | A kind of biologic bracket material for dental root regeneration, preparation method and applications |
| CN107496988A (en) * | 2017-07-20 | 2017-12-22 | 深圳市光明创博生物制品发展有限公司 | A kind of lyophilized dentale powder material and preparation method thereof |
| CN110732040A (en) * | 2018-07-19 | 2020-01-31 | 上海交通大学医学院附属第九人民医院 | bone repair material, method and use |
| CN114795556A (en) * | 2022-05-10 | 2022-07-29 | 图斯邦柯生物科技(苏州)有限公司 | In vitro tooth bone storage and preparation method |
| CN114795556B (en) * | 2022-05-10 | 2023-05-12 | 图斯邦柯生物科技(苏州)有限公司 | In-vitro dental bone storage and preparation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR20100040427A (en) | Curing method using autotooth and treating method of autotooth | |
| KR102251719B1 (en) | Apparatus and method for producing a dental bone graft | |
| RU2609201C1 (en) | Method for obtaining osteoplastic material | |
| EP2641622B1 (en) | Method for producing a bone transplant material | |
| US20120135379A1 (en) | Alveolar bone graft processing method and alveolar bone graft therefrom and method for treatment using alveolar bone graft | |
| Bittencourt et al. | Mineral content removal after Papacarie application in primary teeth: a quantitative analysis | |
| EP1772432A1 (en) | Acidic water producing device, acidic water producing method, and acidic water | |
| CN104147644A (en) | Decalcified dental matrix of mammal and preparation method thereof | |
| JP2013535284A (en) | Processing method of bone grafting material using teeth, and bone grafting material processed by the method | |
| KR101299395B1 (en) | Adjust the proportion of inorganic and organic teeth bone graft processing method | |
| KR101139337B1 (en) | Grafting material for teeth bone and method of it | |
| KR101175051B1 (en) | Block bone graft material utilizing teeth and processing method of it | |
| CN104162185B (en) | A kind of manufacture method of decalcifying tooth matrix | |
| CN110075356A (en) | A kind of plasticity packing material and preparation method thereof for surgical intervention bone defect | |
| Jin et al. | Histomorphometric analysis of contaminated autogenous tooth graft materials after various sterilization | |
| KR20040087438A (en) | Restorative and Grafting Material for Hard Tissue Defect and Fabrication Method of The Same Using Animal Teeth | |
| US7022347B2 (en) | Restorative and grafting material for hard tissue defects prepared from animal teeth | |
| RU2752035C1 (en) | Method for simultaneous production of demineralized dentin and mineral-organic component from teeth | |
| CN1062186C (en) | Decalcifying tooth matrix and making method | |
| WO1996012509A1 (en) | Process for inactivating and eliminating the organic matrix from animal bone for heterotopic xenografts | |
| CN108042850A (en) | A kind of Injectable bone repair material and preparation method thereof | |
| WO2012121448A1 (en) | Bone graft substitute production method | |
| KR102149847B1 (en) | Preparing method for dental restorative material, disinfection method for dental powder, and disinfectant for dental powder | |
| de Mello et al. | Investigation of the main clinical findings of maxillary sinus surgery with platelet-rich fibrin: a systematic review | |
| JPH04114656A (en) | Bone derivative decalcified tooth material and manufacture thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141119 |