JPH04114656A - Bone derivative decalcified tooth material and manufacture thereof - Google Patents

Abstract

PURPOSE:To achieve a higher bone deriving capacity and a higher growing effect of a bone by remaking a composition obtained by decalcifying a tooth removed from a human being to a decalcified tooth material in the form of coarse particle of flake to be applied for lost bone or the like as caused by pustule of a jaw bone and pyorrhea. CONSTITUTION:A tooth 1 removed from a human being is washed with a sterilizing liquid to be immersed into an aqueous 0.6N hydrochloric acid solution for three to five days and after the removal of calcium components contained, it is immersed into a sterilizing liquid 3 for a half day to wash. Thus, a first intermediate product 4 is obtained. Then, the first intermediate product 4 obtained is divided into four pieces 4a, 4a... vertically with a razor blade sterilized in a clean bench to remove a dental pulp and then, cut into pieces in 1/2mm squares to obtain a second intermediate product 5. Moreover, the second intermediate product 5 is crushed with a pestle 7 in a mortar 6 both being sterilized into coarse particles with a diameter thereof 0.05-0.3mm. Here, the crushing is performed adding a sterilizing liquid to prevent a rise in heat. Finally, the coarse particles are held into a small resin bottle 8 disinfected and further, placed into a sterilized bag to freeze dry and preserved being frozen.

Description

Detailed Description of the Invention [Field of Industrial Application] The present invention relates to an osteoinductive demineralized tooth material suitable as a therapeutic material for diseases causing bone defects and bone resorption in dentistry or clinical medicine, and a method for producing the same. .

[Conventional technology]

In order to actively promote bone regeneration for bone diseases that cause bone defects and bone resorption, such as jaw bone cysts and alveolar pyorrhea, there is a treatment technique in which calcium compounds and hydroxyabatite are used to fill the bone. Furthermore, for the same purpose, there is a treatment technique in which bone grafting is performed using autologous bone or allogeneic bone.

In particular, for bone recovery from dental aFN leakage, gingival abrasion is one of the surgical treatments.
This is a technique that uses Goatesox etc. to suppress fibrous growth from the gingiva and to encourage new alveolar bone formation.

However, the prior art as described above has the following drawbacks.

(i) Even if calsulam compounds and hydroxyapatite are filled with bone defects due to bone cysts and alveolar bone resorption due to alveolar pyorrhea, no ability to induce bone has been observed, so bone new generation will not occur. It was reliable, the healing speed was not very fast, and it took a considerable period of time to heal.

(ii) Also, in order to finally heal the bone defect,
Although it is desirable that the filled materials be absorbed by the living body and replaced by the bone, these materials often remain in the living body for a long period of time because the absorption speed of these materials is very slow.

(iii) Demineralized bone has been proven to have osteoinductive ability, and freeze-dried demineralized bovine bone is known. However, when this is applied to the human body, it involves xenotransplantation, which causes problems with antigen-antibody reactions, so it has not been used in actual clinical practice.

(iv) Surgery for alveolar pyorrhea (flap opera)
2. In the technique using Goatesox, it does not have osteoinductive ability itself, so the reliability of treatment is low, and the treatment speed is slow, so a relatively long treatment period is required.

[Problems to be solved by the present invention]

The present invention has been made in view of the actual situation of osteoporosis, and it is known that the inorganic components (mainly calcium) of bones and teeth that have been demineralized and removed have the ability to induce bone formation. The purpose of the present invention is to provide a coarse-grained or flaky demineralized tooth material using one fl tissue obtained by demineralizing extracted human teeth. Among these, it has been particularly applied to bone defects caused by jaw bone cysts and alveolar pyorrhea, with the aim of enhancing its bone induction ability and bone growth effect.

[Means for solving problems]

The gist of the present invention is a. An osteoinductive demineralized tooth product obtained by forming a tissue produced by demineralizing a human extracted tooth into coarse granules or flakes, and a method for producing the same. As,
The following two methods are disclosed. That is, b. In the case of coarse particles, a. An extracted human tooth is washed with sterile water and then immersed in an aqueous solution of about 0.6 N hydrochloric acid for 3 to 5 days to remove the calcium components contained therein, and then soaked in sterile water. A first step in which the first intermediate is obtained by soaking for 1 to 2 days and then washing; a. Divide the first intermediate into quarters lengthwise with a razor blade, remove the pulp, and then cut the tooth substance into 1 to 2 fl cubes. a second step of cutting the second intermediate into mesh shapes, and cutting the second intermediate into diameters of 0405 mm using a mortar and pestle;
E. Osteoinduction, which consists of a third step of making coarse particles of ~0.3 m, and a fourth step of sterilizing the coarse particles, placing them in a predetermined container, freeze-drying, sealing, and freezing storage. (coarse grain)
A method for producing a demineralized dental product, in the case of C6 flakes, a first step of obtaining a first intermediate in the same manner as described in section A above; After dividing and removing the pulp, the second step is to cut the tooth substance into thin pieces with a thickness of about 1 cm to obtain a second intermediate. 2
The third step is to form a flaky material with a thickness of 00 μs, and the fourth step is to process and freeze the flaky material in the same manner as described in step 2 above. This is a method for producing a demineralized tooth article, which is summarized above.

〔Example〕

The present invention will be explained below based on examples and clinical examples with reference to the drawings.

FIGS. 1(i) to (vi) are explanatory diagrams showing the manufacturing process of a coarse-grained demineralized tooth material.

The extracted human tooth 1 shown in (i) is washed with sterile water and then placed in a 0.6 N hydrochloric acid aqueous solution shown in (ii).
After being immersed for 5 days to remove the calcium component contained therein, the first intermediate 4 is obtained by immersing it in sterilized water 3 for 1 to 2 days and then washing, as shown in (iii). This is the first step.

Next, as shown in (1v), the first intermediate 4 obtained was vertically divided into four parts 4a using a sterilized razor blade in a clean bench.
4a... After removing the pulp, as shown in (v), 1-
The second intermediate 5 is obtained by cutting into 21 square dice. This is the second step.

Furthermore, the second intermediate 5 is prepared in a sterilized mortar 6 and pestle 7 (
vi) It is ground into coarse particles with a diameter of 0.05 to 0.3 m. At this time, add sterile water to prevent heat rise. This is the third step.

Finally, the coarse particles are placed in a small sterilized plastic bottle 8, as shown in (vi), and then placed in a sterile bag, freeze-dried, and stored frozen. This is the fourth step.

A clinical example of coarse particles and its results will be described.

One example was filling of a hen's egg-sized canal after cystectomy in the mandible, and the other was filling of the canal of a small requestor after root excision for root granuloma in the anterior maxillary teeth. be. Clinical findings based on observation results for 4 months after surgery showed that the healing period on both sides was shorter than with conventional surgery, and
Postoperative bone new growth was also observed in the radiological findings, and the effect was remarkable.

FIGS. 2(i) to 2(v) are explanatory diagrams showing the manufacturing process of a flaky demineralized tooth article.

The first step for coarse-grained materials also applies to flaky materials. The first intermediate 9 obtained in the first step is vertically divided into two parts using a sterilized razor blade in a clean bench as shown in FIG. 2(i), and after removing the dental pulp, the first intermediate 9 is shown in FIG. 2(ii). A second intermediate body 10 is obtained by cutting the tooth substance into thin pieces having a thickness of about 1 co+. This is the second step.

Furthermore, as shown in FIG. 2(iii), a thin section 12 having a thickness of 30 to 200 μs as shown in FIG. 2(iv) is obtained using a sterilized biological sample thin section preparation device 11 (vibratome). This is the third step. In the figure], 1a indicates the razor blade, and llb indicates the fixing base of the maker.

Finally, the thin slices are treated in the same manner as for the coarse particles and stored frozen (13).The state shown in FIG. 2(v) is the fourth step.

We describe clinical cases of flakes and their results.

The flakes were used clinically in 6 cases. That is, three cases of filling by pasting it on the bone wall of the bone cavity after removal of a maxillary cyst, and flapape ratio for alveolar pyorrhea.
These are three examples of filling a bone defect in the alveolar bone in a laminated manner after turning on. Clinical findings based on observation results for one month after surgery showed that the healing period was shorter in all six cases than with conventional methods, and even in the XvA findings, post-surgery bone regeneration was enhanced, indicating that the effect was It was remarkable.

〔Effect of the invention〕

The present invention is based on the above configuration, and the demineralized dental material made using extracted human teeth has a remarkable effect of increasing bone and curing dental diseases at an early stage due to its osteoinductive ability. In particular, a. Since bone new generation is almost certain, it is possible to increase the success rate of treatment for bone diseases that cause bone defects or bone resorption. b. Because bone new generation occurs early, treatment is possible. Since it is a therapeutic material that is quickly absorbed by the living body, it is replaced by bone without remaining in the living body for a long period of time. -ration, it is expected to suppress fibrillary proliferation from the gingival side and generate new bone, similar to Voretex, and bone new generation will occur by the demineralized tooth material itself, e. During surgery for diseases associated with bone defects. When used, it is an extremely useful invention as it can produce a number of positive results, such as the secondary effect of suppressing diffuse bleeding (oozing) from the bone parenchyma after removal of the lesion. .

[Brief explanation of drawings]

FIGS. 1(i) to (vi) are explanatory diagrams sequentially showing the manufacturing process of the coarse granular demineralized tooth product according to the present invention, and FIGS. 2(i) and (v) are the same flaky demineralized tooth products. FIG. 3 is an explanatory diagram sequentially showing the manufacturing process.

Claims (1)

[Scope of Claims] 1. An osteoinductive demineralized tooth material obtained by forming coarse granules or flakes of tissue produced by demineralizing extracted human teeth. 2. After cleaning the extracted human tooth with sterile water, immerse it in an aqueous solution of about 0.6N hydrochloric acid for 3 to 5 days to remove the calcium components, etc., and then immerse it in sterile water for 1 to 2 days, and then wash it. The first step is to obtain a first intermediate. After dividing the first intermediate into quarters lengthwise with a razor blade and removing the pulp, the tooth substance is cut into dice of 1 to 2 mm square and the second intermediate is obtained. The second step of rinsing and the second
The intermediate body is made into a diameter of 0.05 to 0.3 using a mortar and pestle.
The third step is to make coarse particles of mm size, and the fourth step is to sterilize the coarse particles, put them in a predetermined container, freeze-dry them, seal them, and freeze them.
A method for producing an osteoinductive demineralized tooth article, which comprises the steps of: 3. After washing the extracted human tooth with a sterile aqueous solution, it is immersed in a 0.6N hydrochloric acid solution for 3 to 5 days to remove the calcium component, and then immersed in a sterile aqueous solution for 1 to 2 days, and then washed. The first step is to obtain the first intermediate, and after dividing the first intermediate into two lengthwise with a razor blade and removing the pulp, the tooth substance is cut into approximately 1 cm thick pieces.
a second step of cutting into flakes to obtain a second intermediate;
The intermediate is processed for 30 to 200 μs using a biological sample slicer.
A method for producing an osteoinductive demineralized dental material, which comprises a third step of forming a thick flake, and a fourth step of sterilizing the flake, putting it in a predetermined container, freeze-drying, sealing it, and storing it frozen.