KR102326560B1 - Powder manufacturing method for teeth bone graft and powder for teeth bone graft manufacturied by the method - Google Patents

Abstract

The present invention provides a method for producing a powder for dental bone graft material, which can secure the reliability of a graft material using teeth by having excellent hygiene, and promote excellent bone formation performance by increasing the absorption speed of a graft material, and a powder for dental bone graft material, produced thereby. According to the present invention, the method comprises: a first disinfection step of immersing the teeth in a first disinfectant to first disinfect; a foreign material removal step of removing the foreign material from the disinfected tooth; a tooth cutting step of forming a tooth piece by cutting the tooth from which the foreign material is removed to a predetermined size; a tooth piece drying step of drying the cut tooth piece; a tooth piece grinding step of pulverizing the dried tooth piece to a predetermined size to obtain a base powder; a second disinfection step of secondarily disinfecting the base powder with a second disinfectant and washing with sterile distilled water; a third disinfection step of disinfecting the sterilized base powder with a third disinfectant and washing with sterile distilled water; a residue removal step of removing disinfection residues and impurities remaining in the base powder that has undergone the third disinfection step; and a powder completion step of drying the base powder from which the residues and impurities are removed and sterilizing the same to complete the powder for transplantation material.

Description

Powder manufacturing method for tooth bone graft material and powder for tooth bone graft material manufactured thereby

The present invention relates to a method for manufacturing a powder for a dental bone graft material and a powder for a dental bone graft material manufactured thereby, and more particularly, to have excellent hygiene to ensure reliability of a dental implant material using teeth, and to increase the absorption rate of the implant material. The present invention relates to a method for producing a powder for dental bone graft material capable of promoting excellent bone-forming performance by increasing it, and to a powder for dental bone graft material manufactured thereby.

Recently, techniques for using bone graft materials such as bone powder, bone chips, and bone blocks for bone regeneration or restoration have been developed. Bone graft material fills the space, plays an important role in bridge role and bone healing, and also contains bone morphogenetic protein (BMP) that promotes bone formation, thus promoting treatment. Orthopedic surgery, neurosurgery and dentistry.

In particular, in dentistry, etc., various bone graft materials are used for implant surgery. The first treatment considered as a treatment for restoring tooth loss is the use of implants. Factors affecting the success of implants are similar to those influencing the success of other bone grafts, the patient's condition, the material used. It is affected by the characteristics of the operator and the skill of the operator. Patient conditions that affect the success rate include the age of the patient, the presence or absence of systemic disease, the patient's habits, or the height or bone quality of the remaining alveolar bone. As material factors, factors such as material diameter, length, shape, and surface treatment will affect the success rate. The skill of the operator is also an important factor influencing the success of the procedure. Therefore, various technologies have been developed to increase the success rate of dental implants. Even if the patient's physical condition does not provide ideal conditions for implant surgery, the success rate of implants can be dramatically improved through the development of materials centered on technology.

Since the implant surface is the main site in contact with the bone, a high osteogenesis rate for the bone defect around the implant and a tight junction between the bone and the implant are prerequisites for a successful implant. Therefore, depending on the type of bone graft material used, there is a difference in the rate of bone formation and the bonding between the implant and the bone. Whether or not tight junctions occur quickly will be determined. In general, hydroxyapatite (HA) is a major component of bone and is widely used for surface treatment of implants. There are many reports on the success rate of implants coated with hydroxyapatite. In particular, hydroxyapatite is the same component as human bone and tooth enamel, and has a great effect on the restoration of the defective part of the tooth enamel. is also in the spotlight.

However, opinions are still divided as to which bone graft material is better to use. Bone graft material is literally used to induce bone formation or increase bone by inserting bone graft material into an area that cannot be placed due to lack of gingival bone during implant procedure. When the gum bone is formed through this, dentists who are the practitioners will help the implant surgery.

Currently, bone graft materials can be divided into allogeneic bone graft materials, xenograft materials, synthetic bone graft materials, autologous bone graft materials, and the like.

Allogeneic bone graft material refers to bone obtained from other individuals of the same species, and is usually collected from the bones of a dead person.

Xenogeneic bone graft material refers to a method of transplanting bone tissue obtained from animals of different species, mainly extracted from cattle or calves.

Synthetic bone graft material is a material from which organic lipids have been removed and made from bone substitutes and synthetic materials, not obtained from humans or other animals, and is mainly composed of bioceramics. When allograft and xenograft materials are used, they have the advantage of fast hardening and good bone quality, but there is a disadvantage in that there are problems such as virus infection. When using a synthetic bone graft material, it has the advantages of being easy to purchase and convenient to use, as well as a slow rate of hardening and poor bone quality.

In addition, autologous bone graft material refers to the patient's own bone to be operated on, not from any other individual, and is collected from an intraoral or extraoral donor site. In particular, recently, an autogenous tooth bone graft material that can be used as a bone substitute by processing extracted teeth from a patient has been introduced and used in clinical practice.

This autogenous tooth bone graft material has excellent biosynthesis, is healed by bone induction and bone conduction, and is absorbed after a certain period of time, and has been used for extraction or reconstruction and bone-guided regeneration.

Autologous tooth bone graft materials have been undergoing clinical research and animal experiments on various topics, and have shown somewhat similar results compared to other bone substitutes. In addition, the part about clinical safety was also mentioned, which was that even after rapid bone healing and wound dehiscence was observed, effective secondary healing was possible because of high resistance to infection.

However, in the process of manufacturing autogenous teeth as a bone graft material, a demineralization process is included as in allogeneic bone. This demineralization process removes a significant portion of inorganic components and changes the conditions for bone formation considerably in comparison with non-demineralization. Because the bone formation time and bone mass may vary depending on the conditions, the use of autologous teeth as a bone graft material is limited.

Acid treatment is used to remove mineral components and promote bone-forming protein activation in the case of allogeneic bone, leaving only collagen and non-genic proteins to remove minerals. If demineralization is not performed, freeze-drying is performed to sterilize it and then use it as a graft material. This mineral demineralization removes the supporting minerals in the process of bone regeneration, so the strength is weak, but absorption time can be saved.

As such, in the case of allogeneic bone, there have been studies confirming that the bone-forming ability of the bone graft material is different depending on the degree and method of demineralization. The same can be applied, but in previous studies, it was difficult to evaluate the demineralization efficiency of each acid because there was no data on the degree of mineral demineralization according to the type of acid.

As a prior art using an allogeneic bone graft material, the autogenous tooth graft material proposed in Korean Patent Publication No. 10-1382067 uses only the root portion with weak support, so that the soft tissue around the alveolar bone is filled in the bone defect area, or the bone of the tooth graft material is generated. There was a problem that the formation of the alveolar bone was not smooth as it was absorbed into the human body before it became available.

Republic of Korea Patent Publication No. 10-1382067 (2014.04.04. Announcement) Republic of Korea Patent Publication No. 10-1198115 (2012.11.09. Announcement) Republic of Korea Patent Publication No. 10-1524774 (2015.06.01. Announcement) Republic of Korea Patent Publication No. 10-1386322 (2014.04.17. Announcement)

Therefore, the present invention for solving the above-mentioned problems of the prior art can ensure the reliability of the implant material using the tooth to have excellent hygiene, and increase the absorption rate of the implant material to achieve excellent bone-forming performance. An object of the present invention is to provide a method for manufacturing a powder for re-use and a powder for dental bone graft material manufactured thereby.

The problems to be solved of the present invention are not limited to those mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

According to one aspect of the present invention for achieving the above objects and other features of the present invention, a primary disinfection step of immersing the teeth in a first disinfectant material to primary disinfection; a foreign material removal step of removing the foreign material from the disinfected tooth; a tooth cutting step of forming a tooth piece by cutting the tooth from which the foreign material is removed to a predetermined size; a tooth piece drying step of drying the cut tooth piece; a tooth piece grinding step of pulverizing the dried tooth piece to a predetermined size to obtain a base powder; a second disinfection step of secondarily disinfecting the base powder with a second disinfectant and washing with sterile distilled water; a third disinfection step of disinfecting the sterilized base powder with a third disinfectant and washing with sterile distilled water; a residue removal step of removing disinfection residues and impurities remaining in the base powder that has undergone the third disinfection step; There is provided a powder manufacturing method for dental bone graft material comprising a; drying and sterilizing the base powder from which the residues and impurities are removed to complete the powder for the graft material.

In one aspect of the present invention, the first disinfection step consists of immersing in ethyl alcohol (ethanol) or hydrogen peroxide for 15 minutes in a temperature environment of 25 ° C. This can be done by obtaining

In one aspect of the present invention, the tooth cutting step comprises cutting the tooth piece into a plurality of tooth pieces corresponding to each particle size of the base powder to be formed in the tooth piece grinding step, and the tooth piece drying step is the tooth piece It may consist of hot air drying the piece for 40 minutes to 80 minutes in a temperature environment of 25 ° C to 35 ° C.

In one aspect of the present invention, the tooth piece grinding step includes a first base powder having a particle size of 850 μm to 1000 μm, a second base powder having a particle size of 500 μm to 850 μm, and 250 μm to 500 μm It is preferable to grind to obtain a third base powder having a particle size.

In one aspect of the present invention, in the second disinfection step, ethyl alcohol or hydrogen peroxide is mixed with the base powder and rotated at 230 to 270 rpm for 40 to 80 minutes using a rotary stirrer in a temperature environment of 20° C. or less. After stirring, wash with sterile distilled water, mix 0.6N hydrochloric acid with the washed base powder, and use a rotary stirrer at 8 to 15 ° C. It may consist of maintaining pH 7.0-7.2.

In one aspect of the present invention, the tertiary sterilization step is a mixture of non-acidic hypochlorous acid (HOCL) or ethyl alcohol of pH 5.0 to 6.5 with the base powder that has undergone the secondary sterilization step, and in a temperature environment of 20 ° C. After rotating and stirring at 230 to 270 rpm for 40 to 80 minutes using a rotary stirrer, washing is performed with sterile distilled water, and the step of removing the residue may be centrifuged at 5000 rpm to 7000 rpm for a predetermined time using a low-temperature centrifuge.

In one aspect of the present invention, in the powder completion step, the base powder that has undergone the residue removal step is frozen in a temperature environment of −80° C. for 30 minutes to 1 hour, then vacuum freeze-dried to dry, and then the dried base powder Sterilization at an EO gas concentration of 450-1200 mg/l or sterilization at a gamma irradiation dose of 15-25 kGy.

According to another aspect of the present invention, there is provided a powder for dental bone graft material manufactured by the method for manufacturing a powder for tooth bone graft material according to the above aspect.

According to the method for producing a powder for tooth bone graft material according to the present invention and the powder for tooth bone graft material prepared thereby, the following effects are provided.

First, the present invention has the effect of securing the reliability of the implant material using the teeth by improving hygiene through a specific treatment.

Second, the present invention has the effect of increasing the absorption rate of the graft material to promote excellent bone-forming performance.

Third, the present invention has the effect of being able to transform into various forms such as paste form, particle form, block form, and plate form according to the needs of the consumer.

Effects of the present invention are not limited to those mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

1 is a flowchart showing a method for manufacturing a powder for dental bone graft material according to the present invention.
Figure 2 is a photograph of the tooth pieces cut in the tooth cutting process included in the powder manufacturing method for bone graft material according to the present invention.
3 is a photograph of the powder for bone graft material manufactured by the method for manufacturing powder for bone graft material according to the present invention.
4 is a screen shot with an electron microscope of the surface of the powder for bone graft material manufactured through the method for manufacturing powder for tooth bone graft material according to the present invention.

Additional objects, features and advantages of the present invention may be more clearly understood from the following detailed description and accompanying drawings.

Prior to the detailed description of the present invention, the present invention can make various changes and can have various embodiments, and the examples described below and shown in the drawings are not intended to limit the present invention to specific embodiments. No, it should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention.

When a component is referred to as being “connected” or “connected” to another component, it is understood that the other component may be directly connected or connected to the other component, but other components may exist in between. it should be On the other hand, when it is said that a certain element is "directly connected" or "directly connected" to another element, it should be understood that no other element is present in the middle.

The terms used herein are used only to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise. In this specification, terms such as "comprises" or "have" are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof.

In addition, terms such as "...unit", "...unit", "...module", etc. described in the specification mean a unit that processes at least one function or operation, which includes hardware or software or hardware and It can be implemented by a combination of software.

In addition, in the description with reference to the accompanying drawings, the same components are given the same reference numerals regardless of the reference numerals, and the overlapping description thereof will be omitted. In describing the present invention, if it is determined that a detailed description of a related known technology may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted.

In addition, throughout this specification, when a step is located "on" or "before" another step, this means not only when a step is in a direct time-series relationship with another step, but also includes a step of mixing after each step and Likewise, the order of two stages includes the same rights as in the case of an indirect time series relationship in which the time series order can be changed.

Hereinafter, a method for manufacturing a powder for a dental bone graft material according to a preferred embodiment of the present invention and a powder for a dental bone graft material manufactured thereby will be described in detail with reference to the accompanying drawings.

1 is a flowchart showing a method for manufacturing powder for dental bone graft material according to the present invention, and FIG. 2 is a photograph of tooth pieces cut into three shapes during the tooth cutting process included in the method for manufacturing powder for dental bone graft material according to the present invention. It is a photograph, and FIG. 3 is a photograph of the powder for bone graft material manufactured by the method of manufacturing powder for tooth bone graft material according to the present invention.

The powder manufacturing method for dental bone graft material according to the present invention, as shown in FIGS. 1 to 3, largely includes a primary disinfection step (S100); Foreign matter removal step (S200); tooth cutting step (S300); Tooth piece drying step (S400); tooth piece grinding step (S500); Second disinfection step (S600); the third disinfection step (S700); Residue removal step (S800); Powder completion step (S900); includes.

Specifically, the powder manufacturing method for bone graft material according to the present invention, as shown in Figs. 1 to 3, a primary disinfection step (S100) of immersing a tooth, a raw material, in a first disinfectant material to primary disinfection; a foreign material removal step (S200) of removing foreign substances from the teeth disinfected in the first disinfection step (S100); a tooth cutting step (S300) of cutting the tooth from which the foreign material is removed to a predetermined size in the foreign material removing step (S200); A tooth piece drying step (S400) of drying the tooth piece (teeth piece) cut in the tooth cutting step (S300); a tooth piece grinding step (S500) of obtaining a base powder obtained by pulverizing the tooth piece dried in the tooth piece drying step (S400) to a predetermined size; Secondary disinfection of the base powder obtained by grinding the tooth pieces in the grinding step (S500) with a second disinfectant and washing with sterile distilled water (S600); A third disinfection step (S700) of disinfecting the base powder disinfected in the second disinfection step (S600) with a third disinfectant and washing with sterile distilled water; a residue removal step (S800) of removing disinfection residues and impurities remaining in the base powder that has undergone the third disinfection step (S700); and a powder completion step (S900) of drying and sterilizing the base powder from which residues and impurities are removed in the residue removal step (S800) to complete the powder for transplantation material (S900).

The first disinfection step (S100) consists of immersing a tooth, which is a raw material, in a disinfectant (first disinfectant) having a predetermined temperature to disinfect.

More specifically, the first disinfection step (S100) consists of sterilizing by immersion in ethyl alcohol (ethanol) (preferably 70% ethyl alcohol) or hydrogen peroxide (preferably 70% hydrogen peroxide), and more specifically It is preferable to be immersed in ethyl alcohol (ethanol) or hydrogen peroxide for 15 minutes to 60 minutes in a temperature environment of 25 ° C to 60 ° C.

In the first disinfection step (S100), if the treatment temperature is lower than 25 ℃, the reaction rate is very slow and there is a problem that the entire processing process time is delayed. There is a problem with making

The foreign material removal step (S200) consists of removing the dental caries and pulp tissue. For example, the foreign material removal step (S200) may remove caries and pulp tissue using a handpiece.

Continuing, the tooth cutting step (S300) is to cut the tooth from which the foreign material has been removed in the foreign material removal step (S200) to a predetermined size, this tooth cutting step (S300) is a tooth to be described in detail below. In the piece grinding step (S500), a plurality of base powders having different particle sizes are provided, and these particle sizes are cut into individual tooth pieces to form different base powders, respectively.

Specifically, the tooth cutting step (S300) is followed by using a diamond bur (product name: FG 6856 018, komet, Germany) and a cutting bur (product name: FG 700 010, komet, Germany). The tooth is cut so as to be provided with a plurality of tooth pieces corresponding to each particle size of the base powder to be formed in the tooth piece grinding step (S500), which is a process. The tooth cutting step (S300) of the present invention forms three tooth pieces by setting the teeth to a size of 40%:40%:20%.

And the tooth piece drying step (S400) is a process of drying the tooth piece cut in the tooth cutting step (S300) before grinding in the tooth piece crushing step (S500), using a hot air dryer at 25 ° C. ~60 ℃, preferably 30 ℃ 40 minutes ~ 80 minutes, preferably consists of hot air drying for 60 minutes.

In the drying process of the tooth piece drying step (S400), when the drying temperature exceeds 60° C., there is a problem in that the organic matter of the tooth piece is damaged due to heat, and if it is less than 25° C., there is a problem in that the drying processing time is delayed.

Next, the tooth piece grinding step (S500) is made to obtain a plurality of base powder by grinding the tooth piece dried in the tooth piece drying step (S400) into different particle sizes.

Specifically, the tooth piece crushing step (S500) is a first base powder having a particle size of 850 μm to 1000 μm using a bone crusher, a second base powder having a particle size of 500 μm to 850 μm, and pulverize to obtain a third base powder having a particle size of 250 μm to 500 μm.

Here, the first base powder, the second base powder, and the third base powder are preferably provided in a ratio of 4:4::2.

Preparing the first to third base powders with different particle sizes as described above in the tooth piece grinding step (S500) ensures the formation of new dental bone and maintaining the volume of the bone graft material during transplantation of the graft material. can be obtained

In other words, the absorption rate of the graft material and the degree of bone formation have a proportional relationship, and since an increase in absorbability promotes an increase in bone formation performance, a powder with a relatively small particle size contributes to the formation of new pubis, and relatively large particles The size of the powder contributes to maintaining the volume.

Next, in the second disinfection step (S600), the base powder obtained by grinding the tooth piece crushing step (S500) is secondarily disinfected with a second disinfectant and washed with sterile distilled water, preferably the first to the third base powder is placed in a container (eg, snap tube) and sterilized by putting ethyl alcohol (preferably 70% ethyl alcohol) or hydrogen peroxide (preferably 70% hydrogen peroxide), and then a rotary stirring device (Shaking Incubator) ) at 230 to 270 rpm, preferably 250 rpm, at 10 ° C. to 60 ° C. for 40 to 80 minutes, preferably 60 minutes, followed by repeated washing three times for 3 minutes with sterile distilled water.

When the treatment temperature exceeds 60°C in the process of the secondary disinfection step (S600), there is a problem in that the organic matter of the base powder is damaged due to heat, and when it is less than 10°C, there is a problem in that the processing time is delayed, When the rpm is out of the range, there is a problem in that the reaction speed is slowed down.

In addition, in the second disinfection step (S600), 0.6N hydrochloric acid is mixed with the washed base powder, and then, using a shaking incubator at 230 to 270 rpm, preferably at 250 rpm, 8 ° C to 60 ° C, Preferably, it is carried out at 10° C. for 5 minutes, and then includes maintaining a pH of 7.0 to 7.2 with phosphate buffered saline (PBS: Phosphate Buffered Saline).

In the above process, when the treatment temperature exceeds 60° C., there is a problem in that the organic matter of the washed base powder is damaged due to heat, and when it is less than 10° C., there is a problem in that the processing time is delayed, and when the rpm is exceeded, the reaction rate There is a problem with slowing down.

In the second disinfection step (S600), as well as disinfection of the base powder, cells, blood, and lipid layers involved in immune rejection are removed, and bone morphogenetic protein (BMP), a protein involved in bone induction, is Demineralize to induce activation.

Subsequently, in the third disinfection step (S700), the base powder treated in the second disinfection step (S600) is disinfected with a third disinfectant and washed with sterile distilled water, and the pH 5.0 in the second disinfected base powder ~6.5 of slightly acidic hypochlorous acid water (HOCL) or ethyl alcohol (ethyl alcohol 70%) is mixed, and using a rotary stirring device (Shaking Incubator) at 230 ~ 270 rpm, preferably at 250 rpm 10 ℃ ~ 60 ℃, preferably Preferably, it is carried out at 20° C. for 40 minutes to 80 minutes, preferably for 60 minutes, and then washing with sterile distilled water for 3 minutes twice.

In the treatment process of the tertiary disinfection, when the treatment temperature exceeds 60° C., there is a problem in that the organic matter of the base powder is damaged due to heat. There is a problem in that the reaction rate is slow.

This third disinfection step (S700) removes Escherichia coli, MRSA (super bacteria, apobacterium, norovirus, campylobacter, influenza virus, etc.) through non-acidic hypochlorous acid water.

Next, the residue removal step (S800) is to remove the disinfection residue (salt) and impurities remaining in the previous process, using a low temperature centrifuge (refrigerated centrifuge) at 1 ° C to 10 ° C. 7000 rpm, preferably 6000 rpm consists of performing for 3 minutes.

This residue removal step (S800) also prevents damage to organic matter in the corresponding temperature range, and it is possible to quickly remove disinfection residues (salts) and impurities through the corresponding rpm.

And the powder completion step (S900) is to dry and sterilize the base powder from which the residues are removed in the residue removal step (S800) to complete the final transplant powder, and the residues are removed from the cryogenic freezer at minus 80°C After freezing the powder for 30 minutes to 1 hour, vacuum freeze-drying (vacuum degree 5×10-4 Torr) is performed to dry it, and then sterilize the dried base powder with an EO gas concentration of 450-1200 mg/l or with a gamma irradiation dose of 15-25 kGy. It consists of sterilization.

Here, the inventors of the present invention confirmed that minerals are not affected even by irradiation, carbohydrates, fats, and proteins are not affected even by a dose of 10 kGy or more, and the effect is insignificant even when irradiated with 50 kGy.

The inventors of the present invention have confirmed that the powder for bone graft material prepared through the above-described method for producing powder for tooth bone graft material has a structure capable of promoting excellent new bone formation while having reliable hygiene.

4 shows that the surface of the powder for bone graft material manufactured through the method for manufacturing powder for tooth bone graft material according to the present invention is photographed with an electron microscope, as shown on the screen, it has a three-dimensionally well-connected micropore structure. can

The powder with micropores is much more hydrophilic than the surface without micropores, and the hydrophilicity of the surface plays a very important role in protein adsorption, cell proliferation, and spread. As shown in FIG. 4, the powder for transplant material obtained through the manufacturing process of the present invention promotes adsorption of body fluids and growth factors, etc. into the entire powder having a ternary well-connected micropore structure, thereby preventing the surface and internal pores. Increases cell adhesion and activity to promote new bone formation.

The powder for dental bone graft material manufactured by the method for manufacturing the powder for tooth bone graft material according to the present invention as described above can be used by forming it in the form of a paste, particle, block, bendable plate shape, or the like.

According to the method for manufacturing the powder for tooth bone graft material according to the present invention as described above and the powder for tooth bone graft material manufactured thereby, hygiene can be improved through a specific treatment to ensure the reliability of the tooth graft material, Excellent bone-forming performance can be achieved by increasing the absorption rate of the graft material, and there is an advantage in that it can be transformed into various forms such as a paste form, a particle form, a block form, and a plate form according to the needs of the consumer.

Although the embodiments as described above have been described with reference to the limited drawings, those skilled in the art may apply various technical modifications and variations based on the above. For example, the described techniques are performed in a different order than the described method, and/or the described components of the system, structure, apparatus, circuit, etc. are combined or combined in a different form than the described method, or other components Or substituted or substituted by equivalents may achieve an appropriate result.

The embodiments described in this specification and the accompanying drawings are merely illustrative of some of the technical ideas included in the present invention. Therefore, since the embodiments disclosed in the present specification are for explanation rather than limitation of the technical spirit of the present invention, it is obvious that the scope of the technical spirit of the present invention is not limited by these embodiments. Modifications and specific embodiments that can be easily inferred by those skilled in the art within the scope of the technical idea included in the specification and drawings of the present invention should be interpreted as being included in the scope of the present invention.

S100: 1st stage of disinfection
S200: Foreign matter removal step
S300: tooth cutting step
S400: tooth piece drying step
S500: tooth piece grinding step
S600: Second stage of disinfection
S700: 3rd stage of disinfection
S800: Residue removal step
S900: Complete stage of powder for graft material

Claims (8)

A first disinfection step of immersing the teeth in a first disinfectant material to first disinfect;
a foreign material removal step of removing the foreign material from the first disinfected tooth;
a tooth cutting step of cutting the tooth from which the foreign material is removed to a predetermined size to form a tooth piece;
a tooth piece drying step of drying the cut tooth piece;
a tooth piece grinding step of pulverizing the dried tooth piece to a predetermined size to obtain a base powder;
a second disinfection step of secondarily disinfecting the base powder with a second disinfectant and washing with sterile distilled water;
a third disinfection step of disinfecting the sterilized base powder with a third disinfectant and washing with sterile distilled water;
a residue removal step of removing disinfection residues and impurities remaining in the base powder that has undergone the third disinfection step;
A powder completion step of drying the base powder from which the residues and impurities are removed and sterilizing it to complete the powder for transplantation material;
The first disinfection step consists of immersing in ethyl alcohol (ethanol) or hydrogen peroxide for 15 minutes at a temperature of 25 °C,
The foreign material removal step consists of obtaining a tooth from which caries and pulp tissue of the tooth are removed,
The tooth cutting step includes cutting into a plurality of tooth pieces corresponding to each particle size of the base powder to be formed in the tooth piece grinding step,
The tooth piece drying step is to dry the tooth piece with hot air for 40 minutes to 80 minutes in a temperature environment of 25 ° C to 60 ° C,
The tooth piece grinding step includes a first base powder having a particle size of 850 μm to 1000 μm, a second base powder having a particle size of 500 μm to 850 μm, and a third base powder having a particle size of 250 μm to 500 μm. crushed to obtain
In the second disinfection step, ethyl alcohol or hydrogen peroxide is mixed with the base powder, and after rotating and stirring at 230 to 270 rpm for 40 to 80 minutes at 230 to 270 rpm in a temperature environment of 20 ° C. or less, washing with sterile distilled water , Mix 0.6N hydrochloric acid with the washed base powder, rotate and stir at 230-270 rpm for 5 minutes at 230-270 rpm using a rotary stirrer in a temperature environment of 8-15 ° C. characterized
A method of manufacturing powder for dental bone graft material.
delete delete delete delete According to claim 1,
The tertiary disinfection step is performed by mixing slightly acidic hypochlorous acid (HOCL) or ethyl alcohol of pH 5.0 to 6.5 with the base powder that has undergone the second disinfection step, and using a rotary stirrer in a temperature environment of 20 ° C. After rotating and stirring at 270 rpm for 40 to 80 minutes, it is washed with sterile distilled water,
The step of removing the residue is characterized in that centrifugation for a predetermined time at 5000 rpm ~ 7000 rpm using a centrifuge
A method of manufacturing powder for dental bone graft material.
According to claim 1,
The powder completion step is,
The base powder, which has undergone the residue removal step, is frozen for 30 minutes to 1 hour in a temperature environment of minus 80° C., then vacuum freeze-dried and dried, and then the dried base powder is sterilized with an EO gas concentration of 450-1200 mg/l or a gamma-irradiation dose of 15 Characterized in sterilization with ~25kGy
A method of manufacturing powder for dental bone graft material.
The powder for tooth bone graft material manufactured by the method for manufacturing the powder for tooth bone graft material according to claim 1 .

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