KR101198115B1 - Processing method of an bone grafts with tooth and the bone grafts processed the same - Google Patents

Abstract

The present invention is a bone graft material processing method and the bone graft material according to the improved tooth to be used separately or mixed according to the use, such as alveolar bone graft or alveolar bone reinforcement by processing the extracted tooth by parts The method of claim 1, wherein the bone graft processing method comprises the steps of: (a) cutting a tooth extracted from a patient at the interface of the crown and root; (b) removing the missing and soft tissue and pulp tissue of the crown cut horizontally; (c) rapidly freezing and then grinding the crown tissue into powder having a particle size of 200 μm to 1000 μm; (d) degreasing the powder; (e) dehydrating the powder; And (f) performing a post-treatment including at least sterilization of the degreased and dehydrated powder to produce a bone graft material.

Description

Method for processing bone graft using teeth and bone graft processed by the same {PROCESSING METHOD OF AN BONE GRAFTS WITH TOOTH AND THE BONE GRAFTS PROCESSED THE SAME}

The present invention relates to a bone graft material, more specifically, the bone graft material processing method using the tooth to be used independently or mixed depending on the use, such as alveolar bone graft or alveolar bone reinforcement by separating the extracted tooth by parts And a bone graft processed thereby.

In recent years, the implantation procedure of generalized teeth is changing to implant immediately after extraction, and preservation and augmentation of damaged alveolar bone is essential in implantation.

Bone grafts used for preservation and enhancement of alveolar bone for implantation include synthetic bone, xenograft, or allogeneic bone graft. Such synthetic bone, xenograft, or allogeneic bone grafts do not surpass autologous bone grafts, and most of the grafts used today perform part of autologous bone function by synthesizing minerals, fats, or parts of autologous bone. .

In addition, although the fixture should be firmly fixed for the implant procedure, it is difficult for the patient with congenital or acquired defects in the alveolar bone to firmly fix the fixture for the procedure. In this case, the conventional implant procedure should be preceded by the treatment of congenital or acquired defects of the alveolar bone by implanting a bone graft made of another person's bone or artificial structure into the alveolar bone.

However, a bone graft made of bone of another person has a problem of requiring immunity and a complicated process to eliminate the problem of immunity.

In addition, the bone graft material made of artificial structures has a problem that the bone formation rate is low because it does not contain a bone-forming protein.

When using the autogenous bone to solve the above problems there is a problem such as additional surgery is required and the amount of material is limited.

Therefore, the development of bone graft material that can solve the above problems is urgently needed.

An object of the present invention for solving the above problems is to provide a bone graft processing method using a tooth for processing the tooth discarded after being extracted into a bone graft having bone induction and bone conduction ability such as autologous bone.

It is another object of the present invention to provide a bone graft having at least any one of bone conduction ability and bone conduction ability processed using the above-described bone graft processing method.

It is another object of the present invention to provide a method for processing bone graft using a tooth having a good dental bone production rate while solving the problem of immunity by having the same function and effect as autologous bone graft and bone graft processed by the same. .

Method for processing bone graft material using a tooth according to the present invention comprises the steps of (a) cutting the tooth extracted from the patient at the interface between the crown and root; (b) removing the missing and soft tissue and pulp tissue of the crown cut horizontally; (c) rapidly freezing and then grinding the crown tissue into powder having a particle size of 200 μm to 1000 μm; (d) degreasing the powder; (e) dehydrating the powder; And (f) performing a post-treatment including at least sterilization of the degreased and dehydrated powder to produce a bone graft material.

And, after performing the step (b) may further comprise the step of washing the crown with distilled water for at least 30 minutes.

In the step (c), it is preferable to rapidly freeze the crown tissue for at least 30 minutes with liquefied nitrogen of -160 ° C or less.

The method may further include washing the powder made in the step (c) with distilled water for at least 30 minutes to remove contaminants and residual soft tissue.

And, the powder made in step (c) is a first wash using ultrasonic washing for 5 minutes to 10 minutes using pure or sterile distilled water, the ultrasonic wave for 10 to 30 minutes using the washing solution as hydrogen peroxide The second wash washed with water and the third wash washed with ultrasonic waves for 5 to 10 minutes sequentially using pure or sterile distilled water three times each, the hydrogen peroxide water is 5% to 7% hydrogen peroxide With a concentration, the washing liquid of each washing step is used in a volume of 5 to 10 times the powder, and the washing liquid can be washed with the ultrasonic waves in a temperature atmosphere of about 60 ℃ to 80 ℃.

In the step (d), the degreasing is preferably performed for 3 to 12 hours by a chloroform methanol solution containing chloroform and methanol in a ratio of 1: 1.

And removing suspended fats in the powder by centrifugation after degreasing in step (d). And washing the powder with distilled water after the centrifugation for at least 1 hour.

In the step (e), the dehydration is preferably performed for 30 minutes to 2 hours using neutral ethyl alcohol.

In addition, in the step (f), the post-treatment may include mixing an additive capable of reinforcing bone-inducing ability generated in the root with the powder, wherein the additive removes the soft tissue and pulp tissue from the root. It is preferable to include powder processed by dehydration, degreasing and deliming in the state.

The method of processing a bone graft according to the present invention comprises the steps of: (a) cutting the extracted tooth at the interface between the crown and the root; (b) removing the soft tissue and pulp tissue of the root which are horizontally cut; (c) rapidly freezing and then grinding the root tissue to form a powder having a particle size of 200 μm to 1000 μm; (d) degreasing the powder; (e) demineralizing the powder; (f) dehydrating the powder; (g) degreasing the powder again; And (h) performing a post-treatment including at least sterilization of the degreased and dehydrated powder to prepare a bone graft material.

Here, after the step (b) may further comprise the step of washing the route with distilled water for at least 30 minutes.

In the step (c), it is preferable to rapidly freeze the crown tissue for at least 30 minutes with liquefied nitrogen of -160 ° C or less.

The method may further include washing the powder made in the step (c) with distilled water for at least 30 minutes to remove contaminants and residual soft tissue.

And, the powder made in step (c) is a first wash using ultrasonic washing for 5 minutes to 10 minutes using pure or sterile distilled water, the ultrasonic wave for 10 to 30 minutes using the washing solution as hydrogen peroxide The second wash washed with water and the third wash washed with ultrasonic waves for 5 to 10 minutes sequentially using pure or sterile distilled water three times each, the hydrogen peroxide water is 5% to 7% hydrogen peroxide With a concentration, the washing liquid of each washing step is used in a volume of 5 to 10 times the powder, and the washing liquid can be washed with the ultrasonic waves in a temperature atmosphere of about 60 ℃ to 80 ℃.

In the step (d) and the step (g), the degreasing is preferably performed for 3 to 12 hours using a chloroform methanol solution containing chloroform and methanol in a ratio of 1: 1.

And removing suspended fats in the powder by centrifugation after degreasing in step (d). And washing the powder with distilled water after the centrifugation for at least 1 hour.

In the step (e), the deliming is preferably performed for about 10 minutes to 1 hour with 0.5 N hydrochloric acid in a volume of 5 to 10 times the powder.

In the step (f), the dehydration is preferably performed for 30 minutes to 2 hours using neutral ethyl alcohol.

The post-treatment may include mixing an additive capable of reinforcing bone conduction generated in the crown with the powder, wherein the additive is dehydrated and degreased in a state in which soft and pulp tissues are removed from the crown. It is preferable to include the processed powder.

And bone graft material using the tooth according to the invention is characterized in that it is processed by any one of the methods described above.

According to the present invention, the tooth discarded after extraction can be processed into a bone graft having bone induction and bone conduction ability such as autologous bone, and the graft has good function and effect as autologous bone graft, thereby solving the problem of immunity. There is an effect that the alveolar bone graft can be made to have a bone production rate.

1 is a cross-sectional view of a tooth illustrating the structure of a tooth to be processed according to the present invention.
Figure 2 is a photograph of the tooth extracted for the bone graft processing method using the tooth according to the present invention.
3 is a photograph of a state in which defects such as tooth decay and inflammation of the extracted tooth of FIG. 2 are removed.
4 and 5 are photographs showing a state in which teeth are cut at the interface between the crown and the root.
Figure 6 is a photo of the soft tissue and pulp tissue removed.
Figure 7 is a photograph showing a bone graft using a tooth according to the present invention in a state in which the crown is ground into powder after rapid cooling.
8 is a photograph showing a bone graft using a tooth according to the present invention in a state in which the root is ground into powder after rapid cooling.

The present invention discloses a method for processing teeth extracted from a patient into bone graft material for alveolar bone, which processed bone graft material can be used for transplantation to the patient himself or another patient.

Referring to FIG. 1, the teeth are exposed to the outside of the gum and the visible part is called a crown, and the part in the gum that cannot be seen by the eye is called a root. In addition, the teeth may be classified into enamel, dentin, pulp, cementum, periodontal membrane, and nerve and blood supply, depending on the composition.

Here, the enamel is hard tissue of the outermost part of the crown covering the teeth, composed of 96% minerals and protecting the inside of the teeth. The dentin is the tissue most similar to the bone tissue of the human body, weaker than enamel, stronger than chalky, and pale yellow or yellowish white opaque tissue. Cretaceous, which serves to fix teeth to the alveolar bone, is harder than bone tissue but weaker than enamel and is white or slightly opaque tissue. And, the dimension is a soft tissue located in the center of the tooth and includes a nerve and a blood supply. In addition, the periodontal membrane is a connective tissue that connects the chalky and alveolar bone of the tooth.

Here, enamel, dentin, and chalky can be classified as hard tissue, and pulp, periodontal and nerve and blood supply can be classified as soft tissue.

The above-described dental tissue is similar to autologous bone, but has different components and physical properties for each site, and the parts available for bone graft are enamel, dentin, and chalky enamel, and the enamel is an implant and void filler and a graft expander ( It can be used as a graft expander, has osteoconduction, and dentin and chalky have osteoinduction and osteoconduction.

The above-described tooth of FIG. 1 is horizontally cut into a boundary line between the crown and the root, that is, the interface (CEJ: Cementoenamel junction), the crown and the root are divided into a powder, and the crown processed powder and the root processed powder are different. By having the components, they can be used as implants, either separately or in admixture with each other. Each embodiment uses a tooth extracted as shown in FIG. 2, and in the tooth extracted as shown in FIG. 2, a defect site such as caries or inflammation is preferably removed in advance and processed as shown in FIG. 3.

≪ Embodiment 1 >

An embodiment in which the crown is processed into powder among extracted teeth will be described.

First, the pretreated tooth is extracted as shown in FIG. 3 to remove a defect site such as caries or inflammation, and is cut at the interface between the crown and the root as shown in FIGS. 4 and 5.

The crown is washed with distilled water for at least 30 minutes after removing the soft and pulp tissue as shown in FIG. As shown in FIG. 6, the crown from which the soft and pulp tissues are removed may remain enamel and dentin.

The crown treated as shown in FIG. 6 is rapidly frozen for at least 30 minutes with liquefied nitrogen and then crushed with a grinder to be processed into a powder having a particle size of 200 μm to 1000 μm as shown in FIG. 7. The powder may then be processed to a particle size larger than 1000 μm.

The powder may be washed with distilled water for at least 30 minutes to remove contaminants and residual soft tissue.

In contrast, the powder is a first wash using ultrasonic washing water for 5 minutes to 10 minutes using pure or sterile distilled water, and a second washing and washing liquid washing ultrasonically for 10 to 30 minutes using washing liquid as hydrogen peroxide. The third wash, which is cleaned with ultrasonic waves for 5 to 10 minutes using pure or sterile distilled water, may be sequentially performed three times each to remove contaminants and residual soft tissue. At this time, the hydrogen peroxide water is used to have a hydrogen peroxide concentration of 5% to 7%, the washing liquid of each washing step is used in a volume of 5 to 10 times the powder, the washing liquid is a temperature atmosphere of about 60 ℃ to 80 ℃ In the ultrasonic powder may be washed.

The powder washed as described above is degreased for 3 to 12 hours by a chloroform methanol solution containing chloroform and methanol in a ratio of 1: 1.

The degreased powder may then be removed with suspended fat by centrifugation and then washed with distilled water for at least 1 hour.

The powder degreased as described above is dehydrated for 30 minutes to 2 hours using neutral ethyl alcohol.

As described above, the degreased and dehydrated powder is post-treated, and the post-treatment process includes washing and freeze-drying the degreased and dehydrated powder and sterilizing the freeze-dried powder using radiation or ethylene oxide gas. Can be.

As described above, the powder including enamel and dentin may have osteoconductivity of enamel and osteoinduction ability of dentin. However, since the amount of enamel is higher than that of dentin and does not demineralize the dentin, the powder processed according to the first embodiment has a stronger bone conduction ability.

As described above, the powder processed by degreasing and dehydrating the crown may be used as a bone graft material for alveolar bone graft independently or by mixing an additive capable of reinforcing bone inducing ability generated in the root. At this time, the additive may be used the same powder as the second embodiment to be processed by dehydration, degreasing and deliming in the state in which the soft and pulp tissue of the root is removed.

≪ Embodiment 2 >

An embodiment in which the root of the extracted tooth is processed into powder will be described.

First, the pretreated tooth is extracted as shown in FIG. 3 to remove a defect site such as caries or inflammation, and is cut at the interface between the crown and the root as shown in FIGS. 4 and 5.

Of these, the root is washed with distilled water for at least 30 minutes after removing the soft and pulp tissue as shown in FIG. As shown in Figure 6, the soft tissue and pulp tissue is removed from the root dentin and chalky.

The route treated as shown in FIG. 6 is processed in the same manner as the rapid freezing and pulverization of the crown of the first embodiment to a powder having a particle size of 200 μm to 1000 μm as shown in FIG. 8. The powder may then be processed to a particle size larger than 1000 μm.

The powder may be washed with distilled water for at least 30 minutes as in the first embodiment to remove contaminants and residual soft tissue.

In addition, the powder is first washed with ultrasonic cleaning for 5 minutes to 10 minutes using pure or sterile distilled water as in the first embodiment, and the ultrasonic washing for 10 minutes to 30 minutes using the cleaning solution as hydrogen peroxide Contaminants and remaining soft tissues may be removed by sequentially performing the third wash, which is performed by ultrasonic washing for 5 to 10 minutes, using the second wash and the washing solution as pure or sterile distilled water, three times each. At this time, the hydrogen peroxide water is used to have a hydrogen peroxide concentration of 5% to 7%, the washing liquid of each washing step is used in a volume of 5 to 10 times the powder, the washing liquid is a temperature atmosphere of about 60 ℃ to 80 ℃ In the ultrasonic powder may be washed.

The powder washed as described above is first degreased for 3 to 12 hours by a chloroform methanol solution containing chloroform and methanol in a ratio of 1: 1.

In addition, the primary degreased powder may be removed from the fat by centrifugation and then washed with distilled water for at least 1 hour.

As described above, the primary degreased powder is demineralized with its 5-10 times volume of 0.5N hydrochloric acid for about 10 minutes to 1 hour.

The demineralized powder is dehydrated for 30 minutes to 2 hours using neutral ethyl alcohol.

The dehydrated powder is secondary degreased for 3 to 12 hours by chloroform methanol solution containing chloroform and methanol in a ratio of 1: 1.

As described above, the degreased, demineralized and dehydrated powder is post-treated, and the degreasing, demineralized and dehydrated powder is washed with physiological saline and freeze-dried as a post-treatment process, and the freeze-dried powder is exposed to radiation or ethylene oxide gas. Sterilization may be included.

After deliming in the above-described processing, the powder may have a residual calcium amount of 8% or less, and the hydrochloric acid solution may be 5 ml per 1 g of powder.

As described above, the powder according to the second embodiment of processing the dentin and the chalky may have the bone conduction ability and the osteoinductive ability of the dentin and the chalky. However, the powder according to the second embodiment has a characteristic of having more osteoinductive ability than the powder according to the first embodiment.

As described above, the powder processed by degreasing, deliming, and dehydrating the crown may be used as a bone graft material for alveolar bone graft independently, or may be used as a bone graft material by mixing an additive capable of reinforcing bone conduction generated in the crown. . At this time, the additive may be used the same powder as the first embodiment processed by degreasing and dehydration in a state in which the soft and pulp tissue of the crown is removed.

As described above, the bone graft material may be processed into powder as in the first and second embodiments, and the bone graft material processed according to the present invention may have relatively strong bone induction performance and bone conduction performance according to the processed components for each embodiment. These may be used alone or in combination to be used as bone graft material for the alveolar bone.

The bone graft material according to the present invention processing the tooth extracted from the patient himself can be safely implanted into the alveolar bone to produce and maintain the alveolar bone because there is no immune rejection reaction.

In addition, even when a tooth extracted from another person is processed, the immunosuppressive material can be safely implanted into the alveolar bone.

Therefore, the tooth discarded after conventional extraction can be processed into a bone graft material having the same function and effect as autologous bone graft so that the safety of the alveolar bone graft can be significantly ensured.

Claims (22)

(a) cutting the extracted tooth at the interface of the crown and the root;
(b) removing the missing and soft tissue and pulp tissue of the crown cut horizontally;
(c) rapidly freezing and then grinding the crown tissue into powder having a particle size of 200 μm to 1000 μm;
(d) degreasing the powder;
(e) dehydrating the powder; And
(f) performing a post-treatment comprising at least sterilization of the degreased and dehydrated powder to produce a bone graft material; and
The post-treatment in step (f) is a method for processing bone graft material using a tooth comprising the step of mixing with the powder an additive capable of reinforcing the bone-inducing ability generated in the root.
The method of claim 1,
After the step (b) of performing a step of washing the crown with distilled water for 30 minutes further comprising the method of processing bone graft using a bone.
The method of claim 1,
The step (c) is a method for processing bone graft material using the teeth, characterized in that the rapid freezing of the crown tissue for 30 minutes with liquefied nitrogen.
The method of claim 1,
The method of claim 1, further comprising the step of removing the contaminants and residual soft tissue by washing the powder made in step (c) with distilled water for 30 minutes.
The method of claim 1,
The powder made in the step (c),
First wash to be ultrasonically cleaned for 5 to 10 minutes using pure water or sterile distilled water as washing solution, second wash and sterilization to be washed with the ultrasonic wave for 10 to 30 minutes using hydrogen peroxide solution as washing solution A third wash, which is ultrasonically washed for 5 to 10 minutes using distilled water, is sequentially performed three times each, wherein the concentration of hydrogen peroxide is 5% to 7%, and the washing liquid of each washing step is 5% of the powder volume. To 10 times, and the temperature of the washing solution is 60 ℃ to 80 ℃ processing method of the bone graft using the bone, characterized in that.
The method of claim 1,
In the step (d), the degreasing method of bone graft material using a tooth, characterized in that is performed for 3 to 12 hours by a chloroform methanol solution containing chloroform and methanol in a ratio of 1: 1.
The method of claim 1,
Removing suspended fat in the powder by centrifugation after degreasing of step (d); And
Washing the powder for 1 hour with distilled water after the centrifugation; processing method of bone graft material using a tooth further comprising.
The method of claim 1,
In the step (e), the dehydration is a method for processing bone grafts using the teeth, characterized in that performed for 30 minutes to 2 hours using neutral ethyl alcohol.
delete The method of claim 1,
The additive is a method for processing bone graft material using a tooth, characterized in that the root comprises a powder processed by dehydration, degreasing and demineralization in the state of removing the soft tissue and pulp tissue.
(a) cutting the extracted tooth at the interface of the crown and the root;
(b) removing the soft tissue and pulp tissue of the root which are horizontally cut;
(c) rapidly freezing and then grinding the root tissue to form a powder having a particle size of 200 μm to 1000 μm;
(d) degreasing the powder;
(e) demineralizing the powder;
(f) dehydrating the powder;
(g) degreasing the powder again; And
(h) performing post-treatment comprising at least sterilization of the degreased and dehydrated powder to produce bone graft material;
The post-treatment is a method for processing bone graft material using a tooth comprising the step of mixing with the powder an additive capable of reinforcing the bone conduction generated in the crown.
The method of claim 11,
After the step (b) of performing a step of washing the root with distilled water for 30 minutes further comprising the method of processing bone graft using a bone.
The method of claim 11,
The step (c) is a method for processing bone graft material using a tooth, characterized in that the rapid freezing of the root tissue for 30 minutes with liquefied nitrogen.
The method of claim 11,
The method of claim 1, further comprising the step of removing the contaminants and residual soft tissue by washing the powder made in step (c) with distilled water for 30 minutes.
The method of claim 11,
The powder made in the step (c),
First wash to be ultrasonically cleaned for 5 to 10 minutes using pure water or sterile distilled water as washing solution, second wash and sterilization to be washed with the ultrasonic wave for 10 to 30 minutes using hydrogen peroxide solution as washing solution A third wash, which is ultrasonically washed for 5 to 10 minutes using distilled water, is sequentially performed three times each, wherein the concentration of hydrogen peroxide is 5% to 7%, and the washing liquid of each washing step is 5% of the powder volume. To 10 times, and the temperature of the washing solution is 60 ℃ to 80 ℃ processing method of the bone graft using the bone, characterized in that.
The method of claim 11,
In step (d) and step (g), the degreasing is performed for 3 to 12 hours by chloroform methanol solution containing chloroform and methanol in a ratio of 1: 1. Processing method.
The method of claim 11,
Removing suspended fat in the powder by centrifugation after degreasing of step (d); And
Washing the powder for 1 hour with distilled water after the centrifugation; processing method of bone graft material using a tooth further comprising.
The method of claim 11,
The deliming in the step (e) is a method for processing bone graft using a bone, characterized in that is performed for 10 minutes to 1 hour with 0.5N hydrochloric acid of 5 to 10 times the volume of the powder.
The method of claim 11,
In the step (f), the dehydration is a method for processing bone grafts using the teeth, characterized in that performed for 30 minutes to 2 hours using neutral ethyl alcohol.
delete The method of claim 11,
The additive is a method for processing bone graft material using a tooth, characterized in that the powder is processed by dehydration and degreasing in the state of removing the soft tissue and pulp tissue.
A bone graft material characterized in that it is processed by the method of any one of claims 1 to 8, 10 to 19 or 21.

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