CN1062186C - Decalcifying tooth matrix and making method - Google Patents
Decalcifying tooth matrix and making method Download PDFInfo
- Publication number
- CN1062186C CN1062186C CN95112416A CN95112416A CN1062186C CN 1062186 C CN1062186 C CN 1062186C CN 95112416 A CN95112416 A CN 95112416A CN 95112416 A CN95112416 A CN 95112416A CN 1062186 C CN1062186 C CN 1062186C
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- tooth
- matrix
- people
- decalcifying
- manufacture method
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- Dental Preparations (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a decalcified tooth matrix and a manufacture method thereof, which mainly provides a decalcified tooth matrix with the advantages of simple technology, low cost and excellent performance for the growth and healing of bones in bone surgeries. Materials used in the manufacture method are abandoned human teeth, and pulverized granules of tooth bodies are obtained by the methods of chemolysis, mechanical cutting and mechanical pulverization. The pulverized granules are immerged in 0.65 to 0.72 valent weight of hydrochloric acid solution, and then the pulverized granules are prepared into a needed tooth matrix after a decalcified process for 10 to 16 hours at the temperature of 34 DEG C to 38 DEG C.
Description
The present invention relates to a kind of medical material and preparation method thereof.
Current, in order to seek a kind of orthopaedics therapy material that brings out osteanagenesis, just carrying out bone inductive treatment research in the world, desire to replace and reform existing various bone graftings with this material.The U.S. one scholar induces in the theory at his bone and thinks, in animal and human's class sclerous tissues (bone and tooth), has a kind of bone morphogenetic protein (B, M, P), and it has the function of bringing out the body osteanagenesis.The sclerous tissues that selects fresh, healthy animal or human's class for use is a raw material, remove soft tissue by machining, be ground into fragment, pass through the chemical process degrease after the washing again, then sclerous tissues's fragment is inserted in the 0.6 equivalent hydrochloric acid solution (0.6NHCL), sclerous tissues's substrate is produced in the constant temperature decalcification.This type of sclerous tissues's substrate is after being implanted into body, and wherein the bone morphogenetic protein that is contained then can produce directional induction in the implantation region, makes originally to ask directed merisis skeletonization of cell plastid or chondrocyte, thereby produces osteanagenesis, reaches the purpose that bone is rebuild.Under this theoretical influence, the U.S. one company produces decalcification people bone bone meal at the beginning of the nineties at the end of the eighties, the selected raw material of this product is the human long bone of fresh and healthy, and this raw material sources are difficult for, and also there is the probability of viral infection when using in this product.
The purpose of this invention is to provide a kind of raw material sources are wide, with low cost, technology is simple, serviceability is good decalcifying tooth matrix and its manufacture method.
The object of the present invention is achieved like this: the selected raw material of people's teeth matrix of this decalcification is depleted people's tooth.Its manufacture method is: (1) at first removes the soft tissue of attachment removal on it with people's tooth of collecting with the method for chemolysis.(2) remove part (as the part that rots and enamel is arranged that has no value for use on the tooth body with the method for machine cuts.(3) method with mechanical activation comminution is ground into granule with the tooth body.(4) tooth body granule is presented to submergence in 0.65～0.72 equivalent hydrochloric acid solution, under temperature Celsius 34～38 ℃, placed 10～16 hours, promptly finishing decalcification becomes needed people's teeth matrix.
In the decalcification technology of above-mentioned said manufacture method, the optimum of hydrochloric acid solution is 0.71 equivalent, and the optimum of temperature is 37 ℃, and the optimum of standing time is 12 hours.
The resulting people's teeth matrix of the decalcification technology of above-mentioned said manufacture method, under the condition of 5 ℃ of Celsius temperatures, insert in the 75% ethanol liquid store standby.
This manufacture method raw material sources are wide, and the people's tooth that is pulled out when adopting the department of stomatology, Hospital treatment gets final product, and product cost is low, and technology is simple.Adopt the resulting decalcifying tooth matrix of this method, have good inductivity osteanagenesis effect.Can find out that by the X-ray photograph analysis regeneration obviously is better than decalcification people bone bone meal produced in USA in it.Show that by electron microscope observation in use will there be the problem of viral infection in this decalcifying tooth matrix.Simultaneously, it is raw material that this technology can also be used for other sclerous tissues, makes sclerous tissues's substrate.This decalcifying tooth matrix can be used for treating that the bone that can not heal voluntarily that causes because of various physics and chemistry, pathology reason is damaged, bone structure is rebuild, and mixes use with the artificial bone, to strengthen artificial bone's bone graftings such as growth fastness.
Specific implementation process of the present invention sees also the foregoing invention content part, does not intend tired chatting at this.
Claims (4)
1, a kind of decalcifying tooth matrix, it is by " remove soft tissue and unworthy part on the raw material, pulverize, insert the technology of soaking in the hydrochloric acid solution and make, it is characterized in that: selected raw material is depleted people's tooth.
2, a kind of manufacture method of decalcifying tooth matrix includes and selects materials, pulverizes, soaks, and it is characterized in that:
(1) the depleted people's tooth that at first will collect is removed on it soft group with the method for chemolysis
Knit;
(2) remove the part that has no value for use on the tooth body with the machine cuts method;
(3) method with mechanical activation comminution becomes granule with tooth body powder;
(4) tooth body granule is presented in 0.65～0.72 equivalent hydrochloric acid solution soaks, Celsius
Under 34～38 ℃ the temperature, placed 10～16 hours, promptly finishing decalcification becomes institute
The people's teeth matrix that needs.
3, the manufacture method of a kind of decalcifying tooth matrix according to claim 2 is characterized in that: in the above-mentioned said decalcification technology, the optimum of hydrochloric acid solution is 0.71 equivalent, and the optimum of temperature is 37 ℃, and the optimum of standing time is 12 hours.
4, the manufacture method of a kind of decalcifying tooth matrix according to claim 2 is characterized in that: the resulting people's teeth matrix of above-mentioned said decalcification technology, under the condition of 5 ℃ of Celsius temperatures, insert in the 75% ethanol liquid store standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95112416A CN1062186C (en) | 1995-08-15 | 1995-08-15 | Decalcifying tooth matrix and making method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95112416A CN1062186C (en) | 1995-08-15 | 1995-08-15 | Decalcifying tooth matrix and making method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1116121A CN1116121A (en) | 1996-02-07 |
| CN1062186C true CN1062186C (en) | 2001-02-21 |
Family
ID=5079495
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95112416A Expired - Fee Related CN1062186C (en) | 1995-08-15 | 1995-08-15 | Decalcifying tooth matrix and making method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1062186C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104162185A (en) * | 2013-11-26 | 2014-11-26 | 李成林 | Making method of decalcified human tooth matrix |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102309523B (en) * | 2011-05-18 | 2013-04-17 | 深圳市光明创博生物制品发展有限公司 | Dentoidin sponginum and preparation method thereof |
| CN104147644A (en) * | 2013-11-26 | 2014-11-19 | 李成林 | Decalcified dental matrix of mammal and preparation method thereof |
| CN111658822A (en) * | 2020-04-20 | 2020-09-15 | 上海交通大学医学院附属第九人民医院 | Decalcification method for teeth and acellular decalcification tooth material |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3127270A1 (en) * | 1981-07-10 | 1983-03-24 | Link, Gottlieb, 4950 Minden | Bone substitute implant as base for implantation of an artificial tooth |
-
1995
- 1995-08-15 CN CN95112416A patent/CN1062186C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3127270A1 (en) * | 1981-07-10 | 1983-03-24 | Link, Gottlieb, 4950 Minden | Bone substitute implant as base for implantation of an artificial tooth |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104162185A (en) * | 2013-11-26 | 2014-11-26 | 李成林 | Making method of decalcified human tooth matrix |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1116121A (en) | 1996-02-07 |
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Granted publication date: 20010221 Termination date: 20140815 |
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