CN104147008A - Gelsmium elegans alkaloid monomer application method - Google Patents
Gelsmium elegans alkaloid monomer application method Download PDFInfo
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- CN104147008A CN104147008A CN201410379252.XA CN201410379252A CN104147008A CN 104147008 A CN104147008 A CN 104147008A CN 201410379252 A CN201410379252 A CN 201410379252A CN 104147008 A CN104147008 A CN 104147008A
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- gelsemii elegantis
- herba gelsemii
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Abstract
The invention discloses a gelsmium elegans alkaloid monomer application method, belongs to the technical field of veterinary drugs, animal health care products and animal health care fodders and particularly relates to application of a gelsmium elegans alkaloid monomer in preparing veterinary drugs, animal health care products and animal health care fodders. The gelsmium elegans alkaloid monomer can reduce the morbidity of weaned piglet's yellow-white dysentery, has a significant impact on the development of veterinary drugs, animal health care products and animal health care fodders, and opens up a new field for the comprehensive utilization of gelsmium elegans.
Description
Technical field
The invention belongs to veterinary drug, animal health-care product and health-care feed technical field, be specifically related to the application of Herba Gelsemii Elegantis alkaloid monomer in preparing veterinary drug, animal health-care product and health-care feed.
Background technology
Herba Gelsemii Elegantis is the loganiaceae plant herb of climing rattan recklessly, has another name called Gelsemium elegans Benth., Herba Gelsemii Elegantis, malicious root, large tea medicine etc., and bitter in the mouth, micro-pungent, hot in nature, very toxic, be mainly distributed in the ground such as Zhejiang, Fujian, Guangdong, Guangxi, Hunan, Guizhou, Yunnan.China is very long for the applicating history of Herba Gelsemii Elegantis, and < < legendary god of farming book on Chinese herbal medicine > > records Herba Gelsemii Elegantis " main incised wound, mastalgia, middle aversion to wind, cough with dyspnea, ghost "; Among the people because its toxicity is large, external is always main, energy detumescence and drawing out poison, killing parasites for relieving itching; Herba Gelsemii Elegantis is very large to people's toxicity, is considered to one of the most malicious in the world plant, it is reported, people Herba Gelsemii Elegantis stem and leaf 10g for oral administration or root 2～8g or 10～38 of tender shoots can cause poisoning.Yet, low dose of Herba Gelsemii Elegantis but but has to some animals the effect that promotes growth, as < < Tang materia medica > > cloud " people or poultry Shiqi leaf are all dead, and sheep food is large fertilizer, pig feed can control febrile disease "; < < Compendium of Materia Medica > > once carried: " it is lethal that people eats Herba Gelsemii Elegantis leaf person by mistake, and sheep food Herba Gelsemii Elegantis is large fertilizer "; The conventional medical herbs > > of < < Guangdong Chinese veterinarian records: " Herba Gelsemii Elegantis, often regularly takes this medicine to pig sheep, can stomach invigorating, parasite killing, fattens "; < < Guangxi Chinese medicinal herbal > > records " when Gaster Sus domestica is received when bad, in order to feed, can appetite stimulator "; Therefore, the edible Herba Gelsemii Elegantis of pig sheep is not only nontoxic, and makes in addition its mao of pool smooth, weight gaining and put the effect of pestilence.More existingly studies confirm that Herba Gelsemii Elegantis has antiinflammatory, analgesia, calmness, hypnosis, improved the effects such as immunity of organisms and anti-stress.
Main active component in Herba Gelsemii Elegantis is alkaloid, comprise the 40 various of monomer compositions such as koumine (Koumine), gelsemine (Geliemine), the gelsemicine third of the twelve Earthly Branches (Kouminicine), kouminidine (Kouminidine), kounidine (Kounidine), sempervine (Sempervirine), koumicine (Koumicine) and gelsemicine penta (Koumidine), wherein koumine and gelsemine content are higher, and gelsemicine toxicity in the third of the twelve Earthly Branches is maximum.And koumine and gelsemine are at the rarely seen report of application of preparing aspect veterinary drug, animal health-care product and health-care feed.
Summary of the invention
The object of this invention is to provide a kind of Herba Gelsemii Elegantis alkaloid monomer in the new application of preparing on veterinary drug, animal health-care product and health-care feed.
The application process of Herba Gelsemii Elegantis alkaloid monomer, for the preparation of the preparation that activates pig AHR receptor active.
Described Herba Gelsemii Elegantis alkaloid monomer comprises: one or more in koumine, gelsemine, gelsemicine third of the twelve Earthly Branches, kouminidine, kounidine, sempervine, koumicine and gelsemicine penta.
Described Herba Gelsemii Elegantis alkaloid monomer preferably includes: one or both in koumine, gelsemine.
The preparation of described activation pig AHR receptor active is single preparations of ephedrine or compound preparation.
The application process of Herba Gelsemii Elegantis alkaloid monomer, for the preparation of veterinary drug, animal health-care product or the animal health-care feed for the treatment of ablactational baby pig HUANGBAI(sic) dysentery.
Described Herba Gelsemii Elegantis alkaloid monomer comprises: one or more in koumine, gelsemine, gelsemicine third of the twelve Earthly Branches, kouminidine, kounidine, sempervine, koumicine and gelsemicine penta.
Described Herba Gelsemii Elegantis alkaloid monomer preferably includes: one or both in koumine, gelsemine.
Described veterinary drug and animal health-care product are single preparations of ephedrine or compound preparation.
Described veterinary drug and the dosage form of animal health-care product are oral instant medicine film, oral liquid, tablet, powder, capsule or injection.
Described animal health-care feed is in basal diet, to add one or both of the koumine of 0.1-0.5wt% (preferably 0.2wt%) and gelsemine.
Herba Gelsemii Elegantis alkaloid monomer related in the present invention extracts in natural plants Herba Gelsemii Elegantis, can form compound preparation with other active component, or other adjuvants forms single preparations of ephedrine.
Herba Gelsemii Elegantis alkaloid monomer in the present invention has activated pig AHR significantly as the part of pig AHR (aryl hydrocarbon receptor), and AHR activates the inflammation path that can oppositely regulate NFkB mediation, this may be the main cause that reduces ablactational baby pig HUANGBAI(sic) dysentery sickness rate, these discoveries will produce significant impact to the exploitation of veterinary drug and animal health-care product, for the comprehensive utilization of Herba Gelsemii Elegantis breaks fresh ground.
Advantage of the present invention:
1. what adopt is that Herba Gelsemii Elegantis alkaloid monomer is better for fowl poultry safeties such as pig, sheep, chickens, and its toxicity is lower.
2. Herba Gelsemii Elegantis alkaloid monomer passes through to activate AHR, and then oppositely regulates the inflammation path of NFkB mediation.Herba Gelsemii Elegantis alkaloid monomer not only can suppress mice caused by dimethylbenzene xylene auricle edema, Ovum Gallus domesticus album causes mice foot swelling and mouse peritoneal capillary permeability increases, and alleviates acute inflammatory reaction; Also significantly suppressed wean yellow and white dysentery of piglet sickness rate.
3. because Herba Gelsemii Elegantis alkaloid monomer comes from the effective ingredient in natural plants-Herba Gelsemii Elegantis, side effect is little, possesses good application prospect.
4. the invention discloses Herba Gelsemii Elegantis alkaloid monomer as veterinary drug and animal health-care product, have safety, efficient, side effect is little, can be oral, the advantage such as injectable.
Accompanying drawing explanation
Fig. 1 is koumine, the affect figure of gelsemine on pig AHR activity;
Fig. 2 koumine, gelsemine xylol induced mice auricle edema affect figure;
Fig. 3 koumine, the affect figure of gelsemine on the foot swelling of Ovum Gallus domesticus album induced mice;
Fig. 4 koumine, the affect figure of gelsemine on capillary permeability enhancing;
Fig. 5 koumine, the affect figure of gelsemine on the sickness rate of ablactational baby pig HUANGBAI(sic) dysentery;
Fig. 6 koumine, the affect figure of gelsemine on ablactational baby pig mortality rate.
The specific embodiment
Below in conjunction with specific embodiment, such scheme is further illustrated.Should be understood that these embodiment are not limited to limit the scope of the invention for the present invention is described.The implementation condition adopting in embodiment can experimental condition be done further adjustment, and not marked implementation condition is generally the condition in normal experiment.
Embodiment 1:
Pig AHR plasmid and luciferase reporter gene plasmid co-transfection are entered to HepG2 cell, build pig AHR and activate detection system.By the successful cell of transfection, after cultivation, be divided into 4 groups, every group of 3 Duplicate Samples, add respectively DMSO, 10nM bioxin (AHR activator), 10nM koumine, 10nM gelsemine, effect 24h, adds after fluorescein substrate reactions, with fluorescence detector, measures fluorescence intensity.， bioxin, koumine, gelsemine are compared with DMSO as can be seen from Figure 1, all can significantly activate AHR receptor, but koumine, gelsemine will be lower than bioxin for the activation effect of pig AHR receptor.Jensen etc. find under study for action can be by activating AHR, and then the inflammation path that oppositely regulates NFkB to mediate, reach effect (the Brenda A Jensen of effective inflammation-inhibiting reaction, Rebecca J Leeman, Jennifer J Schlezinger and David H Sherr.Aryl hydrocarbon receptor (AhR) agonists suppress interleukin-6 expression by bone marrow stromal cells:an immunotoxicology study[J] .Environ Health, 2 (1): 16).This shows that koumine and gelsemine may reach by activating AHR the effect of control poultry inflammatory reaction.
Embodiment 2
Research to koumine, gelsemine study on prevention effect in Herba Gelsemii Elegantis alkaloid monomer: choose Kunming male mice, be divided at random 4 groups, be respectively matched group, gelsemicine subgroup, gelsemine group and indomethacin group (the positive group of antiinflammatory), every group of 10 mices.Gastric infusion, the isopyknic normal saline of matched group gavage, every day 1 time, administration 3 days, last administration in mouse right ear 0.05ml/ is only coated with dimethylbenzene after 45 minutes.After 25min, de-neck is put to death, and ears are cut, and with the punching of diameter 8mm card punch, on electronic balance, weighs, and the weight difference of left ear and auris dextra of take is swelling, carries out statistical analysis; After administration 60min, in the right back foot of mice, open up subcutaneous injection 10% egg white solution 0.1ml, after 30min, with capillary tube amplifying device left and right foot, open up volume, two foots of usining are opened up volume difference as swelling; Gastric infusion every day 1 time, continuous 7 days, 30min after last administration, the azovan blue normal saline solution tail vein injection 0.1ml/10g with 2%.The glacial acetic acid 0.2ml/ of lumbar injection 0.6% only, puts to death after 25min simultaneously, with normal saline 5ml, rinses abdominal cavity, by the centrifugal 10min of turn/min of flushing liquor 1000, gets supernatant and at wavelength 590nm place, reads optical density OD value with visible ultraviolet spectrophotometer.By Fig. 2,3,4 can draw koumine, gelsemine xylol induced mice auricle edema, Ovum Gallus domesticus album causes mice foot swelling and mouse peritoneal capillary permeability all has remarkable potentiation, has alleviated acute inflammatory reaction.
Embodiment 3
At a large-scale pig farm; choose the ablactational baby pig of three batches; each batch is divided into three groups; feed respectively basal diet, basal diet+0.2% koumine, basal diet+0.2% gelsemine; 15d continuously feeds; sickness rate and the mortality rate of statistics yellow and white dysentery of piglet, by Fig. 5,6 can find out that koumine, gelsemine can reduce the M & M of ablactational baby pig HUANGBAI(sic) dysentery significantly.
Embodiment 1-3 is only better embodiment of the present invention, not the present invention is done to any pro forma restriction, any any simple modification, equivalent variations and modification that does not depart from technical solution of the present invention content, according to technical spirit of the present invention, above embodiment is done, all belongs in the scope of technical solution of the present invention.
Claims (10)
1. the application process of Herba Gelsemii Elegantis alkaloid monomer, is characterized in that, for the preparation of the preparation that activates pig AHR receptor active.
2. the application process of Herba Gelsemii Elegantis alkaloid monomer according to claim 1, it is characterized in that, described Herba Gelsemii Elegantis alkaloid monomer comprises: one or more in koumine, gelsemine, gelsemicine third of the twelve Earthly Branches, kouminidine, kounidine, sempervine, koumicine and gelsemicine penta.
3. the application process of Herba Gelsemii Elegantis alkaloid monomer according to claim 2, is characterized in that, described Herba Gelsemii Elegantis alkaloid monomer comprises: one or both in koumine, gelsemine.
4. according to the application process of the Herba Gelsemii Elegantis alkaloid monomer described in claim 1 or 2 or 3, it is characterized in that, the preparation of described activation pig AHR receptor active is single preparations of ephedrine or compound preparation.
5. the application process of Herba Gelsemii Elegantis alkaloid monomer, is characterized in that, for the preparation of veterinary drug, animal health-care product or the animal health-care feed for the treatment of ablactational baby pig HUANGBAI(sic) dysentery.
6. the application process of Herba Gelsemii Elegantis alkaloid monomer according to claim 5, it is characterized in that, described Herba Gelsemii Elegantis alkaloid monomer comprises: one or more in koumine, gelsemine, gelsemicine third of the twelve Earthly Branches, kouminidine, kounidine, sempervine, koumicine and gelsemicine penta.
7. the application process of Herba Gelsemii Elegantis alkaloid monomer according to claim 6, is characterized in that, described Herba Gelsemii Elegantis alkaloid monomer comprises: one or both in koumine, gelsemine.
8. according to the application process of the Herba Gelsemii Elegantis alkaloid monomer described in claim 5 or 6 or 7, it is characterized in that, described veterinary drug and animal health-care product are single preparations of ephedrine or compound preparation.
9. the application process of Herba Gelsemii Elegantis alkaloid monomer according to claim 8, is characterized in that, described veterinary drug and the dosage form of animal health-care product are oral instant medicine film, oral liquid, tablet, powder, capsule or injection.
10. according to the application process of the Herba Gelsemii Elegantis alkaloid monomer described in claim 5 or 6 or 7, it is characterized in that, described animal health-care feed is in basal diet, to add one or both of the koumine of 0.1-0.5wt% and gelsemine.
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| CN201410379252.XA CN104147008A (en) | 2014-08-04 | 2014-08-04 | Gelsmium elegans alkaloid monomer application method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021036537A1 (en) * | 2019-08-23 | 2021-03-04 | 福建医科大学 | Use of koumine in preparation of drugs for treating inflammatory bowel disease |
Citations (2)
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|---|---|---|---|---|
| CN1142373A (en) * | 1995-08-04 | 1997-02-12 | 胡新民 | Chinese medicine for breeding pigs |
| CN101972304A (en) * | 2010-10-21 | 2011-02-16 | 张奎昌 | Superfine gelsemium elegans benth powder and preparation method |
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2014
- 2014-08-04 CN CN201410379252.XA patent/CN104147008A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1142373A (en) * | 1995-08-04 | 1997-02-12 | 胡新民 | Chinese medicine for breeding pigs |
| CN101972304A (en) * | 2010-10-21 | 2011-02-16 | 张奎昌 | Superfine gelsemium elegans benth powder and preparation method |
Non-Patent Citations (3)
| Title |
|---|
| 徐克意等: "钩吻总碱的抗炎作用研究", 《中药药理与临床》 * |
| 易金娥等: "钩吻毒素的研究进展", 《湖南环境生物职业技术学院学报》 * |
| 胡骁飞等: "钩吻碱改善动物生产性能机制", 《饲料研究》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021036537A1 (en) * | 2019-08-23 | 2021-03-04 | 福建医科大学 | Use of koumine in preparation of drugs for treating inflammatory bowel disease |
| US11925634B2 (en) | 2019-08-23 | 2024-03-12 | Fujian Medical University | Use of koumine in preparation of medicament for treatment of inflammatory bowel disease |
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Application publication date: 20141119 |