CN104145861A - Black sea bream and red porgy hybridization insemination method - Google Patents
Black sea bream and red porgy hybridization insemination method Download PDFInfo
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- CN104145861A CN104145861A CN201410380553.4A CN201410380553A CN104145861A CN 104145861 A CN104145861 A CN 104145861A CN 201410380553 A CN201410380553 A CN 201410380553A CN 104145861 A CN104145861 A CN 104145861A
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- porgy
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a black sea bream and red porgy hybridization insemination method which comprises the steps that (1) black sea breams are collected as female parents, red porgies are collected as male parents, ova of the female black sea breams are placed in a container, microscopic examination is carried out, then sperms of the male red porgies are placed in the container, and full mixing is carried out; (2) filtered sea water is added, and the sperms are filtered, then filtered sea water is added, standing is carried out, meshes are used for washing the ova, the ova are added into the sea water for standing after being clean by washing, after fertilized ova float up, the floating-up fertilized ova are placed an incubation net cage with sea water, and full incubation is carried out; and (3) after fries get out of membranes, artificial cultivation is carried out, and hybridization fries are obtained. The method has the advantages of being easy to operate, low in cost and high in efficiency, the fertility rate is high, the average fertility rate is 85%, growing is quick, and the method provides a foundation for black sea bream and red porgy hybridization breeding, reciprocal cross generated offspring heterosis comparing and deep new species breeding.
Description
Technical field
The invention belongs to fish hybridization insemination field, the particularly a kind of method of black porgy and porgy hybridized insemination.
Background technology
With cross method, cultivate improved seeds, or utilize hybrid vigour to be called crossbreeding.Hybridization can make biological genetic material Cong Yige colony transfer to another colony, is an important method that increases biomutation.Dissimilar parent is hybridized can obtain reconfiguring of variation proterties, in filial generation, may there is the combination of parents' merit, (for example even there is the merit of super parental generation, the red carp hybridization in upright pond crucian carp and Jiujiang), certainly also may there is parents' the not obvious proterties combination of advantage, or the unexistent inferior position proterties of parents (hybridization of routine bighead and silver carp).Breeding process is exactly will in filial generation repetitive type, select the individuality that meets breeding objective further to cultivate, and even obtains the stable new varieties of merit.
Hybridization can make crossbreed offspring increase variability and heterogeneity, comprehensive parents' merit, produce the unexistent new proterties of some parents, make offspring obtain larger genetic improvement, there is available hybrid vigour, and given play to great function in the breed improvements of fish with in producing, and be one of Basic Ways of fish breeding, be also for cultivating high-quality fingerling effective measures.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method of black porgy and porgy hybridized insemination, and the method is that crossbreeding, the reciprocal cross of black porgy and porgy produces the heterotic comparison of offspring and more deep seed selection new varieties research, provides the foundation.
The method of a kind of black porgy of the present invention and porgy hybridized insemination, comprising:
(1) it is maternal gathering healthy, anosis, sexually matured black porgy, and gathering healthy, anosis, sexually matured porgy is male parent, and the ovum of black porgy raun is put into container, microscopy, then the sperm of porgy milter is also put into container, rock gently abundant mixing;
(2) add filtering sea, activate sperm, rock or stir sperm is fully contacted with ovum; Then add filtering sea standing; With net sheet, wash ovum, after cleaning, add seawater standing, treat fertilized egg floating, fertilized egg the putting into of floating had to the incubation net cage of seawater, inflation hatching;
(3) after prelarva membrane, carry out artificial culture, obtain hybridization fry.
Filtering sea volume in described step (2) is 100-200ml.
The time of repose that adds filtering sea in described step (2) is 20min.
The order number of the net sheet in described step (2) is 60.
The water temperature of the incubation net cage in described step (2) is 18-22 ℃.
beneficial effect
That the present invention has advantages of is simple to operate, cost is low, efficiency is high; Fertilization rate is higher, average out to 85%; Incubation rate is higher, average out to 80%; Growth rapidly; The method is that crossbreeding, the reciprocal cross of black porgy and porgy produces the heterotic comparison of offspring and more deep seed selection new varieties research, provides the foundation.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
(1) it is maternal gathering healthy, anosis, sexually matured black porgy, gathering healthy, anosis, sexually matured porgy is male parent, and the ovum of black porgy raun is put into container, and microscopy is high-quality ovum, again the sperm of porgy milter is also put into container, rock gently abundant mixing;
(2) add 160ml filtering sea, activate sperm, rock or stir sperm is fully contacted with ovum; Then add the standing 20min of filtering sea; With 60 order net sheets, wash ovum, after cleaning, add seawater standing, treat fertilized egg floating, fertilized egg the putting into of floating had to the incubation net cage of 20 ℃ of seawater, inflation hatching;
(3) after prelarva membrane, carry out artificial culture, obtain hybridization fry.
The method brings up to 86% than conventional hybridization method fertilization rate, incubation rate 80%.
Embodiment 2
(1) it is maternal gathering healthy, anosis, sexually matured black porgy, gathering healthy, anosis, sexually matured porgy is male parent, and the ovum of black porgy raun is put into container, and microscopy is high-quality ovum, again the sperm of porgy milter is also put into container, rock gently abundant mixing;
(2) add 150ml filtering sea, activate sperm, rock or stir sperm is fully contacted with ovum; Then add the standing 20min of filtering sea; With 60 order net sheets, wash ovum, after cleaning, add seawater standing, treat fertilized egg floating, fertilized egg the putting into of floating had to the incubation net cage of 18 ℃ of seawater, inflation hatching;
(3) after prelarva membrane, carry out artificial culture, obtain hybridization fry.
The method brings up to 84% than conventional hybridization method fertilization rate, incubation rate 80%.
Claims (5)
1. a method for black porgy and porgy hybridized insemination, comprising:
(1) it is maternal gathering healthy, anosis, sexually matured black porgy, and gathering healthy, anosis, sexually matured porgy is male parent, and the ovum of black porgy raun is put into container, microscopy, then the sperm of porgy milter is also put into container, rock gently abundant mixing;
(2) add filtering sea, activate sperm, rock or stir sperm is fully contacted with ovum; Then add filtering sea standing; With net sheet, wash ovum, after cleaning, add seawater standing, treat fertilized egg floating, fertilized egg the putting into of floating had to the incubation net cage of seawater, inflation hatching;
(3) after prelarva membrane, carry out artificial culture, obtain hybridization fry.
2. the method for a kind of black porgy according to claim 1 and porgy hybridized insemination, is characterized in that: the filtering sea volume in described step (2) is 100-200ml.
3. the method for a kind of black porgy according to claim 1 and porgy hybridized insemination, is characterized in that: the time of repose that adds filtering sea in described step (2) is 20min.
4. the method for a kind of black porgy according to claim 1 and porgy hybridized insemination, is characterized in that: the order number of the net sheet in described step (2) is 60.
5. the method for a kind of black porgy according to claim 1 and porgy hybridized insemination, is characterized in that: the water temperature of the incubation net cage in described step (2) is 18-22 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410380553.4A CN104145861A (en) | 2014-08-04 | 2014-08-04 | Black sea bream and red porgy hybridization insemination method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410380553.4A CN104145861A (en) | 2014-08-04 | 2014-08-04 | Black sea bream and red porgy hybridization insemination method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104145861A true CN104145861A (en) | 2014-11-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410380553.4A Pending CN104145861A (en) | 2014-08-04 | 2014-08-04 | Black sea bream and red porgy hybridization insemination method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106119377A (en) * | 2016-07-06 | 2016-11-16 | 中国水产科学研究院淡水渔业研究中心 | A kind of differentiate the primer of snapper, black porgy and filial generation thereof, test kit and discrimination method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08289695A (en) * | 1995-04-21 | 1996-11-05 | Nakayama Steel Works Ltd | Cultivation-type ultrahigh fish reef and artificial reef utilizing the same |
| JP2002191255A (en) * | 2000-12-26 | 2002-07-09 | Nippon Steel Corp | Artificial fish bank made of cast iron |
| KR20040051742A (en) * | 2002-12-11 | 2004-06-19 | 성낙술 | a brim fishing grounds |
| CN1879473A (en) * | 2006-04-21 | 2006-12-20 | 宁波市海洋与渔业研究院 | A red sea bream and black sea bream hybridized insemination method |
-
2014
- 2014-08-04 CN CN201410380553.4A patent/CN104145861A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08289695A (en) * | 1995-04-21 | 1996-11-05 | Nakayama Steel Works Ltd | Cultivation-type ultrahigh fish reef and artificial reef utilizing the same |
| JP2002191255A (en) * | 2000-12-26 | 2002-07-09 | Nippon Steel Corp | Artificial fish bank made of cast iron |
| KR20040051742A (en) * | 2002-12-11 | 2004-06-19 | 성낙술 | a brim fishing grounds |
| CN1879473A (en) * | 2006-04-21 | 2006-12-20 | 宁波市海洋与渔业研究院 | A red sea bream and black sea bream hybridized insemination method |
Non-Patent Citations (2)
| Title |
|---|
| 江世贵等: "《中国近海鲷科鱼类种质资源及其利用》", 31 May 2012, 海洋出版社 * |
| 蒋宏雷等: "真鲷与黑鲷杂交繁育技术研究", 《渔业现代化》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106119377A (en) * | 2016-07-06 | 2016-11-16 | 中国水产科学研究院淡水渔业研究中心 | A kind of differentiate the primer of snapper, black porgy and filial generation thereof, test kit and discrimination method |
| CN106119377B (en) * | 2016-07-06 | 2019-11-29 | 中国水产科学研究院淡水渔业研究中心 | A kind of primer, kit and discrimination method identifying red porgy, black porgy and its filial generation |
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Application publication date: 20141119 |