CN104126516B

CN104126516B - Multifunctional experimental box for radiobiology

Info

Publication number: CN104126516B
Application number: CN201410393108.1A
Authority: CN
Inventors: 张鹏; 封巍
Original assignee: Zhejiang Cancer Hospital
Current assignee: Zhejiang Cancer Hospital
Priority date: 2014-08-12
Filing date: 2014-08-12
Publication date: 2022-08-30
Anticipated expiration: 2034-08-12
Also published as: CN104126516A

Abstract

The invention discloses a multifunctional experimental box for radiobiology, which consists of a box body and a matched fixed support, wherein the box body consists of a box back plate structure, a box bottom bin structure, a three-dimensional fixed base plate structure, a sterile looping structure, a box chamber, a box side plate and an incident central point; the supporting fixed bolster includes: the fixing bracket for in vitro cell experiment, the inserting piece type fixing bracket, the flip type fixing bracket and the limb end positioning type fixing bracket. The use function of the multifunctional experimental box for the radioorganisms relates to irradiation, dissection, injection, temporary culture and the like, the coverage of a use object is wide, sterile operation and ingenious fixation of an irradiated part can be realized, and the sober state and the survival state of the experimental object are ensured; can fix and irradiate a plurality of animals at the same time, and also shortens the specific operation time of the experiment and applies large dose; the invention has the advantages of reasonable design, convenient and safe operation, environmental protection, and strong practicability and universality.

Description

Multifunctional experimental box for radiobiology

Technical Field

The invention relates to an experimental tool, in particular to a multifunctional experimental box for radiobiology.

Background

The radiation oncology subject has developed rapidly in recent years, and the basic and clinical studies related thereto have also developed rapidly. Generally, the verification of the radiobiological results requires biological (animal) experiments to verify. Most commonly, experimental studies are performed by establishing animal models of tumors. In the experiment, the fixation of the experimental object is a problem, and particularly, the fixation of the small animals is a difficult problem. And the research on the biological radiation auxiliary device at home and abroad is always a blank. The irradiation experiment of the common rat and mouse has lower requirements on environment, rat state and the like, and most of people adopt a relatively intuitive, original and rough mode, and can be generally implemented as long as a fixing method capable of meeting the experiment requirements is adopted. Nude mice, in vitro cells and the like have higher requirements on experimental environment. Nude mice are vulnerable to bacterial, viral and fungal infections due to the lack of T cells, and only a few mice survive a month if exposed to the external environment. In radiotherapy experiments of tumor models of nude mice, the nude mice are required to survive for a long time after being irradiated by radioactive rays in order to compare the survival time of the nude mice under different experimental protocols. Therefore, the nude mice are required to live in a sterile environment before, during and after the experiment. In vitro cell experiments require sterility and have harsh survival conditions such as constant temperature and constant humidity. At present, the experiment with high requirements is lack of related special instruments, and the experiment inevitably has a series of problems of time consumption, labor waste, large error, even failure and the like. It is desirable to develop a device that can achieve multiple functions of sterilization, fixation, absorption (scattering), protection, etc.

The defects and shortcomings exist at present:

1. the experiment operation is complicated, hard, time-consuming: the process of fixing the mouse is complex, the cell multi-plate contrast experiment needs to be irradiated for multiple times, and the process steps are various;

2. the universality is not high, the resource waste is serious: different test tubes or different lead boxes need to be used for mice with different ages, and the manufacturing cost is high;

3. insufficient fixation: the mouse can break loose, the cell culture vessel is easy to overturn in the transfer process, and the culture vessel has the possibility of sliding displacement during irradiation;

4. the arrangement of the illumination field is not accurate enough: the size of the fixed position needs to be adjusted at any time, the time is wasted, the complexity is high, and the artificial errors are increased;

5. poor sterility: the test paper is directly exposed in the outside air during transportation and irradiation, and cannot meet the basic requirements of the experiment;

6. the survival rate of the mouse is low, the mechanical force is difficult to control when the mouse is fixed, the mouse body is easy to be injured, and the mouse is easy to die when contacting the external environment or is killed by anoxic asphyxiation;

7. the environmental protection requirement can not be met: the lead is used in a large amount, the manufacturing cost is high, the environment is polluted, and the disinfectant is used for a high frequency time;

8. the uncertain factors are increased: the method has the advantages that the experimental steps are multiple, links needing sterilization are increased, the sterile degree is not good, the control steps such as constant temperature and the like are complicated, care is required, and the risk coefficient is increased;

9. poor protection of normally non-illuminated areas: the irradiation of normal organs is caused by factors such as displacement and field.

Disclosure of Invention

The invention aims to fill the blank of the prior art and provides a multifunctional experimental box for radiobiology, which can meet various requirements of mouse (nude mouse), middle mouse, rat, in-vitro cell radiation experiments, caudal vertebra injection, special anatomy of animals, aseptic feeding of animals and the like.

The invention aims to realize the technical scheme that the multifunctional experimental box for the radiobiology is composed of a box body and a matched fixed support, wherein the box body is composed of a box back plate structure, a box bottom bin structure, a three-dimensional fixed base plate structure, a sterile looping structure, a box chamber, box side plates and an incident central point; the box rear plate structure forms the rear wall of the box chamber, two side walls of the box chamber are formed by box side plates, the front wall of the box chamber is of an aseptic looping structure, the three-dimensional fixed base plate structure forms the bottom of the box chamber, and a box bottom bin structure is arranged below the three-dimensional fixed base plate structure;

the box back plate structure consists of a box cover, a box cover shaft, an ultraviolet disinfection lamp tube, a lighting lamp tube, a graduated scale, a folding instrument table, a folding shaft, a pull wire, a V-shaped power supply jack, a table top suction device, a back plate and a top plate. The box cover is connected to one side of the top plate through a box cover shaft; the other side of the top plate is intersected and vertical with the back plate; the ultraviolet disinfection lamp tube and the lighting lamp tube are arranged in parallel below the top plate; a scale and a 220V power jack which are vertically arranged are respectively arranged on one side edge and below the rear plate; a folding instrument table is designed in the middle of the left side of the rear plate and is respectively connected with a folding shaft and a pull wire on the rear plate. A magnetic block is arranged in the middle of the free edge of the long shaft of the folding instrument table and is matched with the table top suction device on the back plate;

the bottom bin structure consists of a biological membrane, a biological membrane clamp, a portable gas tank, a gas cylinder nozzle, a valve, a filter bottle gas outlet nozzle, a filter bottle gas inlet nozzle, an air conditioning equipment bin, a ray absorption bin, an electric bin, a No. I storage battery, a No. II storage battery and a transformer; the three bins of the electric bin, the ray absorption bin and the air conditioning equipment bin are arranged at the bottom in parallel; two biomembrane clamps are respectively arranged on two side walls in the ray absorption bin to clamp the biomembranes; the air conditioning equipment bin is positioned on one side of the ray absorption bin and is internally provided with two portable gas tanks and filter bottles, the portable gas tanks are provided with gas cylinder nozzles, gas in and out of the gas cylinders is controlled by valves, and the filter bottles are provided with filter bottle gas outlet nozzles and filter bottle gas inlet nozzles; the electric bin is positioned on the other side of the ray absorption bin, and a No. I storage battery, a No. II storage battery and a transformer are sequentially arranged in the electric bin;

the three-dimensional fixed substrate structure consists of a limiting hole, a fixed substrate, a bottom plate, an electric heating wire, an A-dimensional adjusting knob, an A-dimensional adjusting shaft, a substrate center, an A-dimensional level meter, a dimensional interval plate, a B-dimensional adjusting shaft, a B-dimensional adjusting knob, a B-dimensional level meter and a gas outlet; the bottom plate is rectangular, the A-dimensional adjusting shaft and the B-dimensional adjusting shaft are respectively positioned on two symmetrical shafts of the bottom plate, wherein the length of the symmetrical shaft corresponding to the A-dimensional adjusting shaft is smaller than that of the symmetrical shaft corresponding to the B-dimensional adjusting shaft; a dimension plate is arranged between the dimension A adjusting shaft and the dimension B adjusting shaft; the two ends of the A-dimensional adjusting shaft are respectively provided with an A-dimensional adjusting knob, and the A-dimensional adjusting knobs are semi-cylindrical and are arranged on the bottom plate; b dimension adjusting knobs are arranged at two ends of the B dimension adjusting shaft, are cylindrical and are arranged outside the bottom plate; the fixed base plate is positioned on the A-dimensional adjusting shaft and the B-dimensional adjusting shaft, the center of the fixed base plate is carved with the center of the base plate, and two sides of the center of the fixed base plate are respectively provided with a beam of electric heating wires; a plurality of limiting holes are distributed on the fixed base plate at equal intervals; the long axis of the A-dimensional level meter is parallel to the B-dimensional adjusting shaft, the long axis of the B-dimensional level meter is parallel to the A-dimensional adjusting shaft, and the A-dimensional level meter and the B-dimensional level meter are respectively arranged on two corners of the fixed base plate; the gas outlet is positioned on the corner of the bottom plate;

the sterile sleeve opening structure consists of an inner plate, a bottom plate, a right sleeve opening, a sealing switch, a left sleeve opening, an outer plate, a left sealing cover, a gear, a left rail tooth, a right rail groove, a right sealing cover, a gear shaft, a right rail tooth and a left rail groove; the upper opening of the inner plate is provided with a right sleeve opening and a left sleeve opening, a right rail groove and a left rail groove are obliquely arranged between the right sleeve opening and the left sleeve opening, left rail teeth run in the right rail groove, right rail teeth run in the left rail groove, a gear is meshed between the left rail teeth and the right rail teeth, a gear shaft is vertical to the inner plate and is connected with the gear, a left sealing cover is connected with the end opening of the left rail teeth and is parallel to the inner plate, and a right sealing cover is connected with the end opening of the right rail teeth and is parallel to the inner plate; the outer plate is provided with a right sleeve opening and a left sleeve opening which correspond to the right sleeve opening and the left sleeve opening on the inner plate, and a gear shaft vertical to the inner plate is connected with a sealing switch on the outer plate;

the supporting fixed bolster includes: the fixing device comprises an in vitro cell experiment fixing bracket, an inserting piece type fixing bracket, a flip type fixing bracket and a limb end positioning type fixing bracket;

the in vitro cell experiment fixing support consists of a fixing table top, a support main board, a ray absorption bin, a biological membrane clamp, a fixing base plate, a left limiting structure, a right limiting structure, a front limiting structure, a rear limiting structure, a left auxiliary limiting structure, a right auxiliary limiting structure and a support foot locking structure; the fixed table top is positioned in the middle of the support main board and is divided into an upper layer and a lower layer, the lower layer is a ray absorption bin, a biological membrane and a biological membrane clamp are arranged in the ray absorption bin, and the biological membrane is fixed through the biological membrane clamp; the upper layer is provided with a left limiting structure, a right limiting structure, a front limiting structure, a rear limiting structure and a left auxiliary limiting structure, wherein two sets of front limiting structures and two sets of rear limiting structures are respectively embedded at the edges of the front side and the rear side of the upper layer of the fixed table board; a left auxiliary limiting structure and a right auxiliary limiting structure are embedded in the center of the upper layer of the fixed table top, and the two left and right limiting structures are arranged on the long shaft of the upper layer of the fixed table top and are positioned on two sides of the left and right auxiliary limiting structures;

the inserting piece type fixing support consists of a support main board, a mouse lip hole, a support foot locking structure, a cover, a groove, a chamber cavity, a leg fixing bayonet, a tying column and friction grains; the chamber cavity is arranged on the support main board, a mouse lip part hole is formed in the front wall of the chamber cavity, grooves are formed in the upper edges of the left wall and the right wall of the chamber cavity, the covering insertion pieces are inserted into the grooves, the leg fixing bayonets are located on the rear wall of the chamber cavity, tie columns are arranged at positions corresponding to the leg fixing bayonets, friction grains are arranged on the tie columns, and support locking structures are arranged on two sides of the support main board;

the flip type fixing bracket consists of a bracket main board, a magnetic lock head, a covering plate groove, a mouse osculum hole, a flip shaft, a chamber side board, a magnetic lock column, a lock rod shaft column, a lock rod, a chamber front board, a bracket foot locking structure, a limb end clamping and positioning structure and a barrier type limiting structure; the support locking structures are positioned on two sides of the support main board; four parallel cuboid chamber cavities are arranged on the support main board, two sides of each cuboid chamber cavity are chamber cavity side boards, the front side is a front board, the rear side is a barrier limiting structure, a movable covering board is arranged above the front board, and the support main board is arranged below the front board; a cover plate groove is arranged on the upper edge of the chamber side plate; the center of the front plate is provided with a mouse osculum hole; the upper edge of the front plate is provided with a flip shaft, and the covering plate is connected with the front plate through the flip shaft; three sets of limb end positioning frame structures are distributed at the lower end of the fence type limiting structure at equal intervals; a magnetic lock cylinder is arranged on the right side of the leftmost cuboid chamber, a lock rod shaft column is arranged on the right side of the rightmost cuboid chamber, one end of a lock rod is connected with the lock rod shaft column, and the other end of the lock rod is connected with a magnetic lock head;

the limb end positioning type fixing support consists of a fixing table top, a support main board, a ray absorption bin, a biological membrane clamp, a central body position table, a support center, a tail included angle adjusting shaft, an operation table, a support foot locking structure, a limb end split shaft structure and a limb end clamping and positioning structure; the support locking structures are positioned on two sides of the support main board; the fixed table top is positioned in the middle of the support main board and is divided into an upper layer and a lower layer, the lower layer is a ray absorption bin, and a biological membrane and a fixer biological membrane clamp are respectively arranged in the ray absorption bin; the upper layer is provided with a limb end split shaft structure positioned in the center and sleeve limb end clamping and positioning structures arranged in a radial manner, wherein one sleeve is connected with a tail included angle adjusting shaft; the central body position table and the operating table are positioned above the limb end split shaft structure, and the center of the bracket is marked on the central body position table;

control limit structure by eaves mouth fender needle, main spacing draw-in groove, the spacing clamp splice of arc, main spacing base, main spacing spring, high spacing adjustment handle, high spacing draw-in groove, high spacing card body, the spacing spring of eaves mouth, the spacing draw-in groove of eaves mouth, the spacing base of eaves mouth and the spacing clamp splice of eaves mouth constitute. A main limiting spring and a main limiting base run in the main limiting clamping groove, wherein one end of the main limiting spring is connected with the main limiting base, and the other end of the main limiting spring is fixed at the far end of the main limiting clamping groove; the main limiting base is connected with an arc-shaped limiting clamping block, the middle opening of the clamping block is provided with a height limiting clamping groove, and a height limiting spring, a height limiting adjusting handle and a height limiting clamping body are sequentially arranged in the clamping block in a walking mode; a cornice limiting clamping groove is formed in the cornice limiting clamping block, a cornice limiting base and a cornice limiting spring run in the cornice limiting clamping groove, one end of the cornice limiting spring is connected with the far end of the cornice limiting clamping groove, and the other end of the cornice limiting spring is connected with the cornice limiting base; the cornice limiting clamping groove is connected with the height limiting clamping body through a cornice limiting base; the near-end of the cornice limiting clamping block is provided with a cornice blocking needle. The force application direction of the main limiting spring is from the far end to the near end of the main limiting clamping groove, and the force application direction of the height limiting spring is from top to bottom; the eaves mouth limiting spring force application direction is from the near end to the far end of the eaves mouth limiting clamping groove.

The front and rear limiting structures are composed of cornice blocking needles, main limiting clamping grooves, main limiting bases, main limiting springs, height limiting adjusting handles, height limiting clamping grooves, height limiting clamping bodies, cornice limiting springs, cornice limiting clamping grooves, cornice limiting bases, cornice limiting clamping blocks and strip-shaped limiting clamping blocks; a main limiting spring and a main limiting base run in the main limiting clamping groove, wherein one end of the main limiting spring is connected with the main limiting base, and the other end of the main limiting spring is fixed at the far end of the main limiting clamping groove; the main limiting base is connected with a strip-shaped limiting clamping block, the middle opening of the strip-shaped limiting clamping block is provided with a height limiting clamping groove, and a height limiting spring, a height limiting adjusting handle and a height limiting clamping body are sequentially arranged in the strip-shaped limiting clamping block in a walking mode; the cornice limiting clamping block is provided with a cornice limiting clamping groove, a cornice limiting base and a cornice limiting spring run in the cornice limiting clamping groove, one end of the cornice limiting spring is connected with the far end of the cornice limiting clamping groove, and the other end of the cornice limiting spring is connected with the cornice limiting base; the cornice limiting clamping groove is connected with the height limiting clamping body through a cornice limiting base; the near end of the cornice limiting clamping block is provided with a cornice blocking needle; the force application direction of the main limiting spring is from the far end to the near end of the main limiting clamping groove, and the force application direction of the height limiting spring is from the top to the bottom; the eaves mouth limiting spring force application direction is from the near end to the far end of the eaves mouth limiting clamping groove.

Control supplementary limit structure by eaves mouth keep off needle, high spacing spring, high spacing adjustment handle, high spacing draw-in groove, high spacing card body, the spacing spring of eaves mouth, the spacing draw-in groove of eaves mouth, the spacing base of eaves mouth, eaves mouth limit stop, the spacing clamp splice of bar, folding axle and folding storehouse constitute. The folding shaft is fixed in the folding bin; one end of the strip-shaped limiting clamping block is connected to the folding shaft, and the other end of the strip-shaped limiting clamping block is free; a height limiting clamping groove is formed in the strip-shaped limiting clamping block; a height limiting spring, a height limiting adjusting handle and a height limiting clamp body sequentially travel from bottom to top in the height limiting clamping groove; the top end of the height limiting clamp body is connected with the cornice limiting base; the cornice limiting clamping block is provided with a cornice limiting clamping groove, a cornice limiting base and a cornice limiting spring run in the cornice limiting clamping groove, and one end of the cornice limiting spring is connected with the cornice limiting base and the far end of the cornice limiting clamping groove; the cornice limiting clamping groove is connected with the height limiting clamping body through a cornice limiting base; the near-end of the cornice limiting clamping block is provided with a cornice blocking needle. The force application direction of the height limiting spring is from top to bottom; the spacing spring application of force direction of eaves mouth is for the near-end to the distal end from the spacing draw-in groove of eaves mouth.

The support foot locking structure consists of a handle, handle columns, embedded columns, a left lock tongue and a right lock tongue, wherein the handle columns are arranged on the support main board, the tail ends of the handle columns are connected with the embedded columns, and the top ends of the two handle columns are connected through one handle; a tongue rod column runs in the handle column, and a push rod groove and a tongue rod groove run in the tongue rod column; the bottom of the tongue bar groove is provided with two opposite wedge-shaped springs, and the top of the tongue bar groove is provided with a tongue bar shaft; two tongue rods run in the tongue rod groove, and the tongue rods run along the groove wall of the tongue rod after being sent out by a tongue rod shaft and are dissociated from the lower end opening of the embedded column; the tongue bars are provided with wedge-shaped parts corresponding to the wedge-shaped part springs, and the tail ends of the two tongue bars are respectively connected with a left spring bolt and a right spring bolt; the two wedge-shaped parts sandwich the push ball; a push seat is arranged at the top end in the push rod groove, and the upper part of the push seat is connected with a push seat spring; the inside of the handle is provided with a linkage rod which is connected with the side part of the pushing seat; the bottom surface of the handle is provided with an opening, and an unlocking button is arranged and connected with the linkage rod.

The limb body shaft structure consists of a limb body angle adjusting area, a limb angle loose screw, a limb body shaft, a limb body loose piece, a shaft bin and a limb body split rod; the central part of the operating table is sunken to form a shaft bin, and the limb end split shaft is positioned in the center of the shaft bin; the four limb end split rods are radiated from the center of the shaft, run in limb end split angle adjusting areas around the shaft bin and are connected with the limb end positioning clamp structure outwards; the extremity body angle elastic screw is designed on the extremity body shaft, and the extremity body elastic sheet is distributed on the shaft.

The limb end clamping and positioning structure consists of a positioning clamping groove, a positioning spring, wedge-shaped positioning clamping teeth, a positioning guide rail and a positioning shrinkage clamp structure, wherein the positioning shrinkage clamp structure consists of a left crescent clamp, a right crescent clamp, two crescent clamp shafts, a shrinkage clamp groove, a shrinkage clamp base, a shrinkage clamp spring, a shrinkage clamp main handle, two clamp handle shafts, a positioning base, a clamp handle spring, a positioning clamp handle and a shrinkage clamp outer sleeve; a positioning clamping groove is formed in the positioning guide rail; wedge-shaped positioning clamping teeth are distributed on the inner wall of one side of the positioning clamping groove at equal intervals; the wedge-shaped positioning latch is divided into a thick head and a thin head, and the thick head faces to the far end; the positioning spring is arranged in the positioning clamping groove, one end of the positioning spring is fixed at the far end of the positioning clamping groove, and the other end of the positioning spring is connected with the positioning base of the positioning shrinkage clamp structure; the positioning clamp handle and the shrinkage clamp main handle are fixed on the positioning base through respective clamp handle shafts, and a clamp handle spring is arranged between the positioning clamp handle and the shrinkage clamp main handle; the bottom end of the shrinkage clamp outer sleeve is connected with a shrinkage clamp main handle, a shrinkage clamp groove is arranged in an opening at the upper end, a shrinkage clamp spring is arranged in the shrinkage clamp groove, and the upper end of the shrinkage clamp spring is connected with a shrinkage clamp base; the left crescent clamp and the right crescent clamp are connected with the shrinking clamp base through a crescent clamp shaft.

The fence type limiting structure consists of a middle positioning left knob, a middle positioning right knob, a folding shaft, a side positioning right knob, an upper inner guide rail, an upper guide groove, an upper outer guide rail, a right frame, a side right clamping column, a lower inner guide rail, a lower guide groove, a lower outer guide rail, a guide wheel, a middle right clamping column, a middle limiting stop, a middle left clamping column, a folding clamping piece, a side left clamping column, a left frame, a guide rail shaft, a guide rail rod cap and a side positioning left knob; the upper inner guide rail and the upper outer guide rail are parallel, and both ends of the upper inner guide rail and the upper outer guide rail are respectively connected with the upper parts of the right frame and the left frame; the lower inner guide rail is parallel to the lower outer guide rail, and two ends of the lower inner guide rail are respectively connected with the lower parts of the right frame and the left frame; an upper guide groove is formed between the upper inner guide rail and the upper outer guide rail, a lower guide groove is formed between the lower inner guide rail and the lower outer guide rail, the upper guide groove and the lower guide groove are parallel and opposite, the middle limit is stopped at the middle part of the lower guide groove, and the lower guide groove is divided into a left part and a right part; a side left clamping column, three guide rail shafts and a middle left clamping column are sequentially arranged in the left inner part of the upper guide groove; the folding cards, the folding shafts and the folding cards are sequentially connected among the lateral left clamping column, the three guide rail shafts and the middle left clamping column; the upper end of the side left clamping column is connected with a side positioning left knob, the upper ends of the three guide rail shafts are respectively connected with a guide rail rod cap, and the upper end of the middle left clamping column is connected with a middle positioning left knob; the lower ends of the side left clamping column, the three guide rail shafts and the middle left clamping column are respectively connected with a guide wheel and run on the left side part of the lower guide groove; a right clamping column at the side part, three guide rail shafts and a right clamping column at the middle part are sequentially arranged in the right side of the upper guide groove; the folding cards, the folding shafts and the folding cards are sequentially connected among the side right clamping columns, the three guide rail shafts and the middle right clamping column; the upper end of the side right clamping column is connected with a side positioning right knob, the upper ends of the three guide rail shafts are connected with a guide rail rod cap, and the upper end of the middle right clamping column is connected with a middle positioning right knob; the lower ends of the side right clamping column, the three guide rail shafts and the middle right clamping column are respectively connected with a guide wheel and run on the right side part of the lower guide groove.

The invention has the beneficial effects that: the invention gives consideration to various radiobiological experiment requirements and clinical animal experiment requirements, the use function relates to irradiation, dissection, injection, temporary culture and the like, the use object covers in vitro cells, and rats, nude mice, frogs, rabbits and the like in any posture from 3 weeks to adults, the aseptic operation and the ingenious fixation of the irradiated part can be realized, and the sober state and the survival state of the experiment object are ensured; the invention can fix and irradiate a plurality of animals at the same time, is more convenient for comparison, and also shortens the specific operation time of the experiment and applies large dose; the whole body is made of transparent organic glass material, the operation and observation are visual and clear, and the cleaning and the disinfection are easy; the invention has the advantages of reasonable design, convenient and safe operation, environmental protection, and strong practicability and universality.

Drawings

FIGS. 1 and 2 are schematic views of the structure of the case of the present invention;

FIG. 3 is a schematic view of the case back plate structure 109 of the present invention;

FIG. 4 is a schematic view of a base plate structure 110 of the present invention;

FIGS. 5 and 6 are schematic views of a three-dimensional fixed substrate structure 111 according to the present invention;

fig. 7 and 8 are schematic views of a sterile cuff structure 112;

FIG. 9 is a schematic view of the left and right spacing structure 100 of the present invention;

FIG. 10 is a schematic view of the front and rear spacing structure 101 of the present invention

FIG. 11 is a schematic view of the left and right auxiliary limiting structures 102 of the present invention

FIGS. 12, 13 and 14 are schematic views of the foot locking arrangement 103 of the present invention;

FIG. 15 is a schematic view of the positioning clip structure 104 of the present invention;

figure 16 is a schematic view of an extremity holding and positioning structure 106 in accordance with the present invention

Figure 17 is a schematic representation of an extremity body axis configuration 105 of the present invention

Fig. 18 and 19 are schematic views of the barrier restraining structure 107 of the present invention;

FIGS. 20 and 21 show a supporting frame of the present invention, in vitro cell experiment fixing frame;

FIG. 22 is a mating bracket of the present invention, a tab-style mounting bracket;

FIG. 23 is a mating bracket of the present invention, a clamshell style fixed bracket;

FIG. 24 is a mating support of the present invention, an extremity positioning type fixed support;

in the figure, a cornice blocking needle 1, a main limit clamping groove 2, an arc-shaped limit clamping block 3, a main limit base 4, a main limit spring 5, a height limit spring 6, a height limit adjusting handle 7, a height limit clamping groove 8, a height limit clamping body 9, a cornice limit spring 10, a cornice limit clamping groove 11, a cornice limit base 12, a cornice limit clamping block 13, a strip-shaped limit clamping block 14, a folding shaft 15, a fixed table surface 16, a folding chamber 17, a support main board 18, an unlocking button 19, a ray absorption chamber 21, a handle 23, a handle column 24, a ring stop surface 25, an embedding column 26, a left spring tongue 27, a right spring tongue 28, a wedge-shaped part spring 29, a biomembrane 30, a biomembrane clamp 31, a wedge-shaped part 32, a tongue rod groove 33, a tongue rod 34, a push rod 35, a push ball 36, a push rod groove 37, a tongue rod column 38, a limit hole 39, a fixed base plate 40, a linkage rod 41, a push seat spring 42, a tongue rod shaft 43, a push seat assembly 44, The left crescent clamp 45, the right crescent clamp 46, the crescent clamp shaft 47, the clamping groove 48, the clamping base 49, the clamping spring 50, the main clamping handle 51, the clamping handle shaft 52, the positioning base 53, the clamping handle spring 54, the positioning clamping handle 55, the clamping sleeve 56, the positioning clamping groove 57, the positioning spring 58, the wedge-shaped positioning clamping tooth 59, the positioning guide rail 60, the magnetic locking head 71, the covering plate 72, the covering plate groove 73, the mouse osculum 74, the flip cover shaft 75, the chamber side plate 76, the magnetic locking column 77, the locking rod shaft column 78, the locking rod 79, the middle positioning left knob 80, the middle positioning right knob 81, the folding shaft 82, the side positioning right knob 83, the upper inner guide rail 84, the upper guide groove 85, the upper outer guide rail 86, the right frame 87, the side right clamping column 88, the lower inner guide rail 89, the lower guide rail 90, the lower outer guide rail 91, the guide wheel 92, the middle right clamping column 93, the middle limit stop 94, the left clamping column 95, the folding clamping column 96, the side left clamping column 97, the side clamping column 97, the middle limit clamp column 95, The device comprises a left frame 98, a guide rail shaft 99, a left-right limiting structure 100, a front-back limiting structure 101, a left-right auxiliary limiting structure 102, a bracket foot locking structure 103, a positioning shrinkage clamp structure 104, an extremity split shaft structure 105, an extremity clamping and positioning structure 106, a barrier type limiting structure 107, a guide rail rod cap 130, a side positioning left knob 131, a chamber front plate 132, a covering plate 133, a groove 134, a chamber 135, a leg fixing bayonet 136, a tie column 137, a friction line 138, an inner plate 139, a bottom plate 140, a right sleeve opening 141, a sealing switch 142, a left sleeve opening 143, an outer plate 144, a left sealing cover 145, a gear 146, a left rail tooth 147, a right rail groove 148, a right sealing cover 149, a gear shaft 150, a right rail tooth 151, a left rail groove 152, an electric heating wire 153, an A dimension adjusting knob 154, an A dimension adjusting shaft 155, a base plate center 156, an A dimension level 157, a dimension plate 158, a B dimension adjusting shaft 159, a B dimension adjusting knob 160, a B dimension level 161, A gas outlet 185, an upper end I, a lower end II, a proximal end III, and a distal end IV.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

Example 1: box structure of multifunctional experimental box for radiobiology

Referring to fig. 1 and 2, the structure is a device for providing sterility, constant temperature, protection, transportation and fixation for experiments, and is the main structure of the invention. The device is composed of a box back plate structure 109, a box bottom bin structure 110, a three-dimensional fixed base plate structure 111, a sterile sleeve opening structure 112, box side plates 179, a box chamber 180 and an incidence central point 186. The rear box plate structure 109 forms the rear wall of the box chamber 180, the two side walls of the box chamber 180 are formed by box side plates 179, the front wall of the box chamber 180 is provided with a sterile sleeve opening structure 112, the three-dimensional fixed base plate structure 111 forms the bottom of the box chamber 180, and the bottom bin structure 110 is arranged below the three-dimensional fixed base plate structure 111. The center of the upper surface of the chamber 180 has an incident center 186 formed thereon after the radiation field is vertically projected.

Referring to fig. 3, the cabinet rear plate structure 109 constitutes a rear wall of the cabinet 180. The structure comprises a box cover 162, a box cover shaft 163, an ultraviolet disinfection lamp tube 164, a lighting lamp tube 165, a graduated scale 166, a folding instrument table 167, a folding shaft 168, a pull wire 169, a 220V power supply jack 170, a table-board sucker 187, a rear plate 188 and a top plate 189. The cover 162 is connected to one side of the top plate 189 by a cover shaft 163 to realize the up-and-down turning; to further ensure sterility, the cover 162 is designed as a solid structure with triangular sides that can overlap with the case side panels 179 when closed, thereby increasing sealing. The other edge of top plate 189 intersects and is perpendicular to back plate 188; the ultraviolet sterilizing lamp 164 and the illuminating lamp 165 are arranged in parallel below the top plate 189, and before and after the experiment, sterile sterilization can be performed. The rear plate 188 is provided with a vertical arrangement of the graduated scale 166 and the 220V power jack 170 on one side and below, respectively, and the graduated scale 166 is used for setting the irradiation depth and improving the accuracy of the irradiation dose. The 220V power jack 170 is used for being externally connected with an alternating current power supply to charge the

storage batteries

183 and 182 in the box bottom bin structure 110. Folding apparatus platform 167 has been designed to the left side middle part position of back plate, and folding apparatus platform 167 can realize respectively that the upset is folding and the horizontal braking through folding axle 168 and the stay wire 169 on connecting back plate 167, and the effect of this structure is that can put various experimental apparatus temporarily, convenient operation to reduce the number of times of business turn over box, realize that the aseptic environment in the box room 180 does the assurance. The middle part of the free edge of the long shaft of the folding instrument table 167 is provided with a magnetic block which is matched with the table board suction device 187 on the back plate, so that the folding instrument table can be folded freely, the space is saved, and the field is neat and tidy.

Referring to fig. 4, the bin base structure 110 forms the bottom of the bin chamber 180. The structure comprises a biological membrane 30, a biological membrane clamp 31, a portable gas tank 171, a gas bottle nozzle 172, a valve 173, a filter bottle gas outlet nozzle 174, a filter bottle 175, a filter bottle gas inlet nozzle 176, an air conditioning equipment bin 177, a ray absorption bin 178, an electric bin 181, a No. I storage battery 182, a No. II storage battery 183 and a transformer 184. The electric bin 181, the ray absorption bin 178 and the air conditioning equipment bin 177 are arranged at the bottom in parallel, two biological film clamps are respectively arranged on two side walls in the ray absorption bin 178, and the biological film clamps can be freely opened and closed to clamp the biological film 30; the air conditioning equipment bin 177 is positioned at one side of the ray absorption bin 178, two portable gas tanks 172 and filter bottles 175 are arranged in the air conditioning equipment bin, and the portable gas tanks 171 provide oxygen or nitrogen and other gases required by an oxygen-rich or oxygen-poor environment; the filter bottle 175 functions to filter (purify) the gas that is about to enter the chamber 180. A valve 173 on the portable gas canister 172 controls the release and closing of gas in the gas cylinder nozzle 172, and a filter cylinder outlet nozzle 174 and a filter cylinder inlet nozzle 176 are designed on the filter cylinder 175. In use, the cylinder nozzle 172 is connected by a hose to the filter bottle inlet nozzle 176 and the filter bottle outlet nozzle is also connected by a hose to an air supply tube outlet 185 located in the housing 180. The electric bin 181 is positioned at the other side of the ray absorption bin 178, and a No. I storage battery 182, a No. II storage battery 183 and a transformer 184 are sequentially arranged in the electric bin. The

storage batteries

183 and 182 provide the electric energy requirement for the whole box body during transition (or without an external power supply), and the transformer 184 is a charging transformer for the

storage batteries

183 and 182.

Referring to fig. 5 and 6, the three-dimensional fixed base plate structure 111 forms the bottom of the cabinet 180, and is composed of a position limiting hole 39, a fixed base plate 40, a bottom plate 140, a heating wire 153, an a-dimensional adjusting knob 154, an a-dimensional adjusting shaft 155, a base plate center 156, an a-dimensional level 157, a dimensional partition plate 158, a B-dimensional adjusting shaft 159, a B-dimensional adjusting knob 160, a B-dimensional level 161, and an air supply pipe outlet 185. The A-dimension adjusting shaft 155 and the B-dimension adjusting shaft 159 are mutually perpendicular and crossed at the center of the bottom plate 140, a dimension plate 158 is arranged between the two shafts, and the dimension plate 158 is used for increasing the contact surface of the two shafts and is not interfered with each other during adjustment. An A-dimensional adjusting knob 154 is arranged at two ends of the A-dimensional adjusting shaft 155, a B-dimensional adjusting knob 160 is arranged at two ends of the B-dimensional adjusting shaft 159, the B-dimensional adjusting knob 160 is arranged outside the box and is of a cylindrical structure, and the A-dimensional adjusting knob 154 is designed in the box and is of a semi-cylindrical structure for saving space and facilitating adjustment. The fixed base plates 40 are distributed on the A-dimensional adjusting shaft in an equal way, the center of the base plate 156 is carved in the central position, and the projection of the center of the base plate 156 and the incident center 186 of the outer surface of the box body coincide, so that the field distribution is convenient to position. Two sides of the center 156 of the base plate are respectively provided with a bunch of heating wires 153, and the circuits of the heating wires 153 are connected with a No. II storage battery 183 in the electric bin 181, so that constant temperature conditions can be provided for the box chamber 180 after heating. A plurality of limiting holes 39 are equidistantly distributed on the fixed base plate, and the limiting holes 39 are embedded in the foot locking structures 103 of all the fixing supports (such as the inserting piece type fixing support 107). The major axis of the A-dimensional level gauge 157 and the B-dimensional adjusting axis are parallel to one corner of the fixed base plate 40 and used for observing the horizontal adjusting condition in the control direction of the A-dimensional adjusting axis 155, and the major axis of the B-dimensional level gauge 161 and the A-dimensional adjusting axis 155 are parallel to the other corner of the fixed base plate 40 and used for observing the horizontal adjusting condition in the control direction of the B-dimensional adjusting axis 159. The outlet 185 of the gas supply pipe is positioned at the corner of the bottom plate 140, which is convenient for conveying oxygen, nitrogen and other gases to realize an oxygen-enriched or oxygen-deficient environment.

Referring to fig. 7 and 8, sterile cuff structure 112 forms a front wall of enclosure 180. The component consists of an inner plate 139, a bottom plate 140, a right sleeve opening 141, a sealing switch 142, a left sleeve opening 143, an outer plate 144, a left sealing cover 145, a gear 146, a left rail tooth 147, a right rail groove 148, a right sealing cover 149, a gear shaft 150, a right rail tooth 151 and a left rail groove 152. The inner plate 139 is provided with a right sleeve opening 141 and a left sleeve opening 143, a right rail groove 148 and a left rail groove 152 are obliquely arranged between the two sleeve openings, a left rail tooth 147 and a right rail tooth 151 run in the corresponding grooves, a gear 146 is meshed between the two rail teeth, a gear shaft 150 is connected with the gear 146 in a manner of being vertical to the inner plate 139, a left sealing cover 145 is connected with the left rail tooth 147 and is parallel to the inner plate 139, and a right sealing cover 149 is connected with the right rail tooth 151 and is parallel to the inner plate 139. The outer plate 144 is opened with a right sleeve opening 141 and a left sleeve opening 143, and is correspondingly communicated with the right sleeve opening 141 and the left sleeve opening 143 opened on the inner plate 139, and a gear shaft 150 vertical to the inner plate 139 is connected with a sealing switch 142 on the outer plate 144. The sealing switch 142 is rotated, the gear shaft 150 drives the gear 146, the gear 146 pushes the left rail tooth 147 and the right rail tooth 151 meshed with the gear to slide left and down and right and up respectively, the circular left sealing cover 145 and the circular right sealing cover 149 simultaneously and respectively shield the left sleeve opening 143 and the right sleeve opening 141, and sealing is completed.

Example 2: the invention relates to a matched bracket (1. in vitro cell experiment fixing bracket, 2. inserting piece type fixing bracket, 3. turning cover

Limb end positioning type fixing support 4) structure

The support kit of the present invention is composed of several substructures, some of which have similar functions, and the substructures will now be described one by one:

referring to fig. 9, the present invention provides a left and right position limiting structure 100. The structure is a special structure on an in vitro cell experiment fixed support, and the two structures are divided on a long shaft of the fixed plate 18. The device mainly realizes the limiting and fixing function in the left and right (and up and down) directions of a target fixture, and can realize the front and back limiting simultaneously when a cell culture dish with radian is used. Control limit structure 100 by eaves mouth keep off needle 1, main spacing draw-in groove 2, the spacing clamp splice of arc 3, main spacing base 4, main spacing spring 5, high spacing spring 6, high spacing adjustment handle 7, high spacing draw-in groove 8, high spacing card body 9, the spacing spring 10 of eaves mouth, the spacing draw-in groove 11 of eaves mouth, the spacing base 12 of eaves mouth and the spacing clamp splice 13 of eaves mouth constitute. A main limiting spring 5 and a main limiting base 4 run in the main limiting clamping groove 2, wherein one end of the main limiting spring 5 is connected with the main limiting base 4, and the other end of the main limiting spring is fixed at the far end of the main limiting clamping groove 2; the main limiting base 4 is connected with an arc-shaped limiting clamping block 3, the middle opening of the clamping block is provided with a height limiting clamping groove 8, and a height limiting spring 6, a height limiting adjusting handle 7 and a height limiting clamping body 9 are sequentially arranged in the clamping block in a walking mode; an eaves limiting clamping groove 11 is formed in the eaves limiting clamping block 13, an eaves limiting base 12 and an eaves limiting spring 10 run in the eaves limiting clamping groove 11, one end of the eaves limiting spring 10 is connected with the far end of the eaves limiting clamping groove 11, and the other end of the eaves limiting spring is connected with the eaves limiting base 12; the cornice limiting clamping groove 11 is connected with the height limiting clamping body 9 through a cornice limiting base 12; 13 the near-end of the spacing clamp splice of eaves mouth is provided with eaves mouth and keeps off needle 1. The force application direction of the main limiting spring 5 is from the far end to the near end of the main limiting clamping groove 2, and the left and right limiting functions are realized by generating elastic force acting on the target object in the left and right directions through the arc-shaped limiting clamping block 3; the force application direction of the height limiting spring 6 is from top to bottom, and the force in the up-and-down direction of the target object is acted through the cornice limiting clamping block 13, so that the up-and-down limiting function is realized; the force application direction of the cornice limiting spring 10 is from the near end to the far end of the cornice limiting clamping groove 11, and auxiliary limiting in the up-down and left-right directions of a target object is realized by combining the cornice blocking needle 1; the arc design of the arc limiting clamping block 3 is specially used for limiting and fixing the front and back directions when a circular cell culture dish is used. In the three-dimensional direction, each limiting clamping block plays a role in limiting and fixing the target object.

Referring to fig. 10, a front and rear spacing structure 101 of the present invention. The structure is a special structure on an in vitro cell experiment fixed support. The '101 front and back limiting structures' are positioned at two sides of the transverse shaft of the 18 support plates, and two sets of the limiting structures are arranged at each side, and the number of the limiting structures is 4. The structure is designed for clamping two cell culture dishes simultaneously, and can realize the limit in the front-back (up-down) direction. The front and rear limiting structure 101 is composed of a cornice blocking needle 1, a main limiting clamping groove 2, a main limiting base 4, a main limiting spring 5, a height limiting spring 6, a height limiting adjusting handle 7, a height limiting clamping groove 8, a height limiting clamping body 9, a cornice limiting spring 10, a cornice limiting clamping groove 11, a cornice limiting base 12, a cornice limiting clamping block 13 and a strip-shaped limiting clamping block 14; a main limiting spring 5 and a main limiting base 4 run in the main limiting clamping groove 2, wherein one end of the main limiting spring 5 is connected with the main limiting base 4, and the other end of the main limiting spring is fixed at the far end of the main limiting clamping groove 2; the main limiting base 4 is connected with a strip-shaped limiting clamping block 14, the middle opening of the strip-shaped limiting clamping block 14 is provided with a height limiting clamping groove 8, and a height limiting spring 6, a height limiting adjusting handle 7 and a height limiting clamping body 9 are sequentially arranged in the height limiting clamping groove; the cornice limiting clamping block 13 is provided with a cornice limiting clamping groove 11, a cornice limiting base 12 and a cornice limiting spring 10 run in the cornice limiting clamping groove 11, one end of the cornice limiting spring 10 is connected with the far end of the cornice limiting clamping groove 11, and the other end of the cornice limiting spring is connected with the cornice limiting base 12; the cornice limiting clamping groove 11 is connected with the height limiting clamping body 9 through a cornice limiting base 12; the near end of the cornice limiting clamping block 13 is provided with a cornice blocking needle 1; the force application direction of the main limiting spring 5 is from the far end to the near end of the main limiting clamping groove 2, and the elastic force in the front-back direction of the acting target object is generated through the strip-shaped limiting clamping block 14, so that the front-back limiting function is realized; the force application direction of the height limiting spring 6 is from top to bottom, and the force in the up-and-down direction of the target is acted by the cornice limiting clamping block 13, so that the up-and-down limiting function is realized; the spacing spring 10 application of force direction of eaves mouth is for the near-end to the distal end from the spacing draw-in groove 11 of eaves mouth, combines the eaves mouth to keep off needle 1 and realizes the supplementary spacing to the target object from top to bottom, fore-and-aft direction.

Referring to fig. 11, the left and right auxiliary limit structures 102 of the present invention are specific structures of the "in vitro cell experiment fixing bracket". The structure is located in the center of the support plate and embedded in the support main plate 18. The structure is mainly designed for clamping small specifications or two cell culture dishes. Control supplementary limit structure 102 by the eaves mouth keep off needle 1, high spacing spring 6, high spacing adjustment handle 7, high spacing draw-in groove 8, high spacing card body 9, the spacing spring 10 of eaves mouth, the spacing draw-in groove 11 of eaves mouth, the spacing base 12 of eaves mouth, eaves mouth limit stop 13, the spacing clamp splice 14 of bar, folding axle 15 and folding storehouse 17 constitute. The folding shaft 15 is arranged in the folding bin 17, and the folding shaft 15 can rotate around the long axis of the support main board 18; one end of the strip-shaped limiting clamping block 14 is connected to the folding shaft 15, so that the strip-shaped limiting clamping block 14 is fixed without displacement in the left and right directions, and the other end of the strip-shaped limiting clamping block 14 is free; a height limiting clamping groove 8 is formed in the strip-shaped limiting clamping block 14; a height limiting spring 6, a height limiting adjusting handle 7 and a height limiting clamp body 9 are sequentially arranged in the height limiting clamping groove 8 from bottom to top; the top end of the height limiting clamp body 9 is connected with an eave limiting base 12; the cornice limiting clamping block 13 is provided with a cornice limiting clamping groove 11, a cornice limiting base 12 and a cornice limiting spring 10 run in the cornice limiting clamping groove 11, and one end of the cornice limiting spring 10 is connected with the cornice limiting base 12 and the far end of the cornice limiting clamping groove 11; the cornice limiting clamping groove 11 is connected with the height limiting clamping body 9 through a cornice limiting base 12; the near-end of the cornice limiting clamping block 13 is provided with a cornice blocking needle 1. The force application direction of the height limiting spring 6 is from top to bottom; the spacing spring of eaves mouth 10 application of force direction is for the near-end to the distal end from the spacing draw-in groove of eaves mouth 11, combines the eaves mouth to keep off needle 1 and realizes the supplementary spacing to the target object about, the left and right directions. The connection of the bar-shaped limiting clamping blocks 14 and the folding shafts 15 enables the bar-shaped limiting clamping blocks 14 to be fixed in the left-right direction without displacement, and the bar-shaped limiting clamping blocks are matched with the left-right limiting structure 100 on one side to achieve the limiting and fixing functions of the target object. The strip-shaped limiting clamping blocks 14 can be accommodated into the folding bin 17 through the folding shaft 15, and fixed positions (spaces) are vacated for fixing single cell culture dishes.

Referring to fig. 12, 13 and 14, the foot locking structure 103 of the present invention is designed on each fixing bracket, and is a connecting device of the fixing bracket and the box body. The support foot locking structure 103 is composed of a handle 23, handle columns 24, an embedded column 26, a left bolt 27 and a right bolt 28, wherein the handle columns 24 are installed on the support main board 18, the tail ends of the handle columns are connected with the embedded column 26, and the top ends of the two handle columns 24 are connected through the handle 23. When in use, the embedded columns 26 are embedded into the limiting holes of the fixed substrate 40, so that limiting movement in the front-back, left-right and 2-dimensional directions is realized; because the diameter of the 26 embedded column is smaller than that of the 24-handle column, and the diameter of the 26 embedded column is larger than that of part of the contact surface to form a 25-ring stop surface, the function of the ring stop surface is to prevent the embedded column from sliding downwards without limit, and realize the downward single-direction limit; a tongue rod column 38 runs in the handle column 24, and a push rod groove 37 and a tongue rod groove 33 run in the tongue rod column 38; the bottom of the tongue bar groove 33 is provided with two opposite wedge-shaped springs 29, and the top is provided with a tongue bar shaft 43; two tongue rods 34 run in the tongue rod groove 33, and the tongue rods 34 run along the wall of the tongue rod groove 33 after being sent out by a tongue rod shaft 43 and are dissociated at the lower end opening of the embedded column 26; the tongue bars 34 are provided with wedge-shaped parts 32 corresponding to the wedge-shaped part springs 29, and the tail ends of the two tongue bars 34 are respectively connected with the left bolt 27 and the right bolt 28; the wedge spring 29 applies a force to the two wedges 32, so that the two tongue bars 34 are close to each other, i.e. the left tongue 27 and the right tongue 28 are close to each other. A push ball 36 is clamped between the two wedge-shaped parts 32; a push seat 44 is arranged at the top end in the push rod groove 37, and the upper part of the push seat 44 is connected with a push seat spring 42; a linkage rod connection 41 is arranged in the handle 23, and the side part of the push seat 44 is connected with the linkage rod 41; the bottom surface of the handle 23 is provided with an opening, an unlocking button 19 is arranged, and the unlocking button 19 is connected with the linkage rod 41. The urging force of the pusher spring 42 is applied to the pusher 44 indirectly to the pusher 35, and finally the pusher 36 is continuously forced downward. The force of the push seat spring 42 is always greater than that of the wedge-shaped part spring 29 which is decomposed in the wedge-shaped part 32 to form upward elastic force, namely the push ball 36 is always positioned at the lower end of the wedge-shaped part 32 under the condition of no other external force, so that the left lock tongue 27 and the right lock tongue 28 are always in a separated state, the outer edges of the left lock tongue 27 and the right lock tongue 28 exceed the limiting hole 39 on the fixed base plate 40, the locking function is realized, and the locking in the last direction, namely the upward direction, is completed. When unlocking is required: when an upward force (the gripping force of the palm of a typical person) is applied to the unlocking button 19, that is, the downward elastic force of the pushing seat spring 42 is reduced to be smaller than the upward elastic force formed by the decomposition of the wedge spring 29 in the wedge 32, the pushing ball 36 is pushed upward, the tongue bars 34 are close to each other, finally, the left locking tongue 27 and the right locking tongue 28 are close to each other, and the outer edges of the left locking tongue 27 and the right locking tongue 28 move into the hole edges of the limiting holes 39 on the fixing base plate 40, so that the unlocking is realized.

Referring to fig. 15, the positioning shrinkage clamp structure 104 of the present invention, the positioning shrinkage clamp structure 104 is composed of a left crescent clamp 45, a right crescent clamp 46, two crescent clamp shafts 47, a shrinkage clamp groove 48, a shrinkage clamp base 49, a shrinkage clamp spring 50, a shrinkage clamp main handle 51, two clamp handle shafts 52, a positioning base 53, a clamp handle spring 54, a positioning clamp handle 55 and a shrinkage clamp jacket 56; a positioning clamping groove 57 is formed on the positioning guide rail 60; the positioning clamping groove 57 is divided into an upper layer and a lower layer, the left layer and the right layer of the upper layer are V-shaped, and the positioning clamping handle 55 can freely position and slide; the lower layer accommodates the positioning base 53 for the positioning base 53 to slide freely. Wedge-shaped positioning latch teeth 59 are equidistantly distributed on the inner wall of one side of the upper layer of the positioning clamping groove 57, the wedge-shaped positioning latch teeth 59 are divided into thick heads and thin heads, and the thick heads face to the far ends; the design facilitates the fixed one-way positioning of the clamping target object, and the positioning grade is clear. The positioning spring 58 is disposed in the positioning slot 57, and has one end connected to the distal end of the positioning slot 57 and the other end connected to the positioning base 53 of the positioning locking clip structure 104, and the spring has a force direction from the distal end to the proximal end, so that the positioning base 53 is always located in the wedge-shaped positioning latch 59. The positioning clamp handle 55 and the shrinkage clamp main handle 51 are fixed on the positioning base 53 through respective clamp handle shafts 52, and a clamp handle spring 54 is arranged between the positioning clamp handle 55 and the shrinkage clamp main handle 51, and the purpose of the spring is to ensure that the two clamp handles are in an open state and tightly attached to the V-shaped edge on the positioning clamping groove 57. The bottom end of the contraction clamp outer sleeve 56 is connected with a contraction clamp main handle 51, a contraction clamp groove 48 is arranged in an opening at the upper end, a contraction clamp spring 50 is arranged in the contraction clamp groove 48, and the upper end of the contraction clamp spring 50 is connected with a contraction clamp base 49; the left crescent clamp 45 and the right crescent clamp 46 are connected with a contraction clamp base 49 through respective crescent clamp shafts 47. The direction of acting on power of lock clamp spring 50 is from the top to the lower extreme, and left crescent clamp 45 and right crescent clamp 46 follow and contract and press from both sides base 49 and descend in contracting clamp groove 48 under the effect of elasticity, because left crescent clamp 45 and right crescent clamp 46 are the arc design, contract and press from both sides the opening part of groove 48 and produce inside crowded power to the clamp gradually, lead to two clamps to draw close each other, more toward descending, draw close more to, thereby produce the fastening clamping action to the target object. The device can clamp the extremities of small extremity animals such as extremities and caudal vertebra, and can be positioned at a certain position for a long time.

Referring to fig. 16, it is an extremity clamping and positioning structure 106 of the present invention, which is suitable for the "flip cover type fixing bracket" and the "extremity positioning type fixing bracket" and has the function of clamping and fixing the extremity of the four limbs experimental subject. The structure comprises a 57 positioning clamping groove, a 58 positioning spring, a 59 wedge-shaped positioning clamping tooth, a 60 positioning guide rail and a 104 positioning locking clamp structure. The limb end clamping and positioning structure 106 is composed of a positioning clamping groove 57, a positioning spring 58, wedge-shaped positioning clamping teeth 59, a positioning guide rail 60 and a positioning locking clamp structure 104, wherein the positioning retraction clamp structure 104 is composed of a left crescent clamp 45, a right crescent clamp 46, two crescent clamp shafts 47, a retraction clamping groove 48, a retraction clamp base 49, a retraction clamp spring 50, a retraction clamp main handle 51, two clamp handle shafts 52, a positioning base 53, a clamp handle spring 54, a positioning clamp handle 55 and a retraction clamp outer sleeve 56; a positioning clamping groove 57 is formed on the positioning guide rail 60; the positioning clamping groove 57 is divided into an upper layer and a lower layer, the left layer and the right layer of the upper layer are V-shaped, and the positioning clamping handle 55 can freely position and slide; the lower layer accommodates a positioning base 53 for the positioning base 53 to slide freely. Wedge-shaped positioning latch teeth 59 are equidistantly distributed on the inner wall of one side of the upper layer of the positioning clamping groove 57, the wedge-shaped positioning latch teeth 59 are divided into thick heads and thin heads, and the thick heads face to the far ends; the design facilitates the fixed one-way positioning of the clamping target object, and the positioning grade is clear. The positioning spring 58 is disposed in the positioning slot 57, and has one end connected to the distal end of the positioning slot 57 and the other end connected to the positioning base 53 of the positioning locking structure 104, and the spring has a force direction from the distal end to the proximal end, so that the positioning base 53 is always located in the wedge-shaped positioning latch 59.

Referring to fig. 17, there is shown an extremity split shaft structure 105 of the present invention. The structure is a special component of a 'limb end positioning type bracket', and is positioned in the center of a 16-fixed table top for achieving the purpose of limb end folding and unfolding of an experimental object. The limb body shaft structure 105 is composed of a limb angle adjusting area 64, a limb angle tightening screw 65, a limb body shaft 66, a limb body tightening sheet 67, a shaft bin 68 and a limb body rod 69. The central part of the operating platform 70 is recessed to form a shaft bin 68, and the limb end body shaft 66 is positioned in the center of the shaft bin 68; four limb-end split rods 69 radiate from the center of the shaft, run around the limb-end angle adjustment zone 64 of the shaft bin 68, and are externally connected to limb-end positioning clamp structures 106. The 4 69 limb body separating rods can freely move in the 64 limb body separating angle adjusting area, the separation degree is set, normal organs are convenient to protect, and the dosage of the target area is increased. The extremity angle elastic screw 65 is designed on the extremity body shaft 66, and the extremity body elastic sheet 67 is distributed on the shaft. Along with the habit of the user, the rotating tightness of the limb end split rod 69 can be adjusted, so that the fixing effect and convenient operation are ensured.

Referring to fig. 18 and 19, there is a barrier restraint structure 107 of the present invention. The structure is a special component of a 'fence type fixing bracket', and has the functions of blocking, limiting and fixing. The folding device is composed of a middle positioning left knob 80, a middle positioning right knob 81, a folding shaft 82, a side positioning right knob 83, an upper inner guide rail 84, an upper guide groove 85, an upper outer guide rail 86, a right frame 87, a side right clamping column 88, a lower inner guide rail 89, a lower guide groove 90, a lower outer guide rail 91, a guide wheel 92, a middle right clamping column 93, a middle limit stop 94, a middle left clamping column 95, a folding clamping sheet 96, a side left clamping column 97, a left frame 98, a guide rail shaft 99, a guide rail rod cap 130 and a side positioning left knob 131. The structure is of a 'mouth' structure as a whole, and the folding barrier in the shape of a door curtain is arranged in the structure, so that the non-irradiation part is effectively blocked. The upper inner guide rail 84 and the upper outer guide rail 86 are parallel, and both ends of the upper inner guide rail and the upper outer guide rail are respectively connected with the upper parts of a right frame 87 and a left frame 98; the lower inner rail 89 and the lower outer rail 91 are parallel and both ends are connected to the lower portions of the right frame 87 and the left frame 98, respectively. An upper guide groove 85 is formed between the upper inner guide rail 84 and the upper outer guide rail 86, a lower guide groove 90 is formed between the lower inner guide rail 89 and the lower outer guide rail 91, the upper guide groove 85 and the lower guide groove 90 are parallel and opposite, a middle limit stop 94 is arranged in the middle of the lower guide groove 90, and the lower guide groove 90 is divided into a left part and a right part. The left side of the upper guide groove is internally and sequentially provided with a left clamping column 97 at the side part, three guide rail shafts 99 and a left clamping column 95 at the middle part in a penetrating way; four sets of folding cards 96, folding shafts 82 and folding cards 96 are sequentially connected among the side left clamping column 97, the three guide rail shafts 99 and the middle left clamping column 95; the upper end of the side left clamping column 97 is connected with a side positioning left knob 131, the upper ends of the three guide rail shafts 99 are respectively connected with a guide rail rod cap 130, and the upper end of the middle left clamping column 95 is connected with a middle positioning left knob 80; the lower ends of the side left clamping column 97, the three guide rail shafts 99 and the middle left clamping column 95 are respectively connected with a guide wheel 92 and moveTo the left side of the lower channel 90; the right side is also distributed. The upper edges of the upper guide grooves 86 are flush, and the upper outer guide rails 86 are higher than the upper inner guide rails 84 at the lower edges, so that the unidirectional limiting strengthening effect is achieved on the target object. The lower inner guide rail 89 and the lower outer guide rail 91 are mutually formed by edges

In this configuration, guide roller 92 is kept sliding left and right in lower guide groove 90. The posts and rods are pivotally connected to each other by a fold-over tab 96 for axial rotation relative to each other. The left side positioning knob 131, the left middle positioning knob 80, the right middle positioning knob 81 and the right side positioning knob 83 are respectively connected with corresponding clamping columns, and can be screwed in and locked at the upper edge position of the upper guide groove 85. The rail cap 130 is attached to the rail shaft 99 and the visor rides on the upper outer rail 86 and the upper inner rail 84 for free sliding movement. The space formed by the side right clamping column 88 and the right frame 87 is mainly used for limiting the right leg of the subject mouse; the space formed by the middle left clamping column 95 and the middle right clamping column 93 is mainly used for limiting the caudal vertebra of the subject mouse or the leg of the mouse; the space formed by the lateral left clip 97 and the left frame 98 is mainly a left leg limit of the subject mouse.

Referring to fig. 20 and 21, the present invention is a supporting frame of the present invention, which is a fixing frame for in vitro cell experiment, and is composed of a fixing table 16, a main plate 18 of the supporting frame, a radiation absorbing chamber 21, a biological film 30, a biological film clamp 31, a fixing substrate 40, a left and right limiting structure 100, a front and back limiting structure 101, a left and right auxiliary limiting structure 102, and a supporting frame foot locking structure 103; the fixed table 16 is positioned in the middle of the support main board 18, the fixed table 16 is divided into an upper layer and a lower layer, the lower layer is a ray absorption bin 21, a biological membrane 30 and a biological membrane clamp 31 are arranged in the ray absorption bin 21, and the biological membrane 30 is fixed through the biological membrane clamp 31; the upper layer is provided with a left-right limiting structure 100, a front-back limiting structure 101 and a left-right auxiliary limiting structure 102, wherein two sets of front-back limiting structures 101 are respectively embedded in the edges of the front side and the back side of the upper layer of the fixed table top 16; the upper layer of the fixed table 16 is embedded with a left and right auxiliary limiting structure 102, and two left and right limiting structures 100 are installed on the long axis of the upper layer of the fixed table and located at two sides of the left and right auxiliary limiting structures 102.

Referring to fig. 22, it is a supporting frame of the invention, namely a plug-in sheet type fixed frame. The structure adopts the principle of fixed chamber spacing, fixed barrier blocking and limb end splitting, realizes the functions of braking, blocking and positioning, and is suitable for contrast irradiation experiments of a plurality of young mice (nude mice) with the body lengths of 5-7 CM and the like. The fixed bracket is composed of a bracket main board 18, a mouse and osculum hole 74, a bracket foot locking structure 103, a cover 133, a groove 134, a chamber 135, a leg fixing bayonet 136, a tie post 137 and friction grains 138. Five independent chamber cavities 135 are arranged in a row on the main support plate 18, and the size of the chambers is just suitable for mice in any posture between 3 weeks and 8 weeks. The upper part of the chamber 135 is completely open and the closure is obtained by pulling in a groove 134 which covers the passage of the tab 133. The front wall of the chamber 2 is provided with a nude mouse osculum hole 74 with the diameter of 1.5CM, and the nude mouse osculum part can exchange breath with the outside through the hole. The right third of the rear wall of the chamber 135 is missing to form a strip-shaped gap called as a leg fixing bayonet 136, which just accommodates the extension of the rear leg of the next nude mouse, a tie column 137 for fixing the rear leg of the nude mouse is corresponding to the leg fixing bayonet 136, friction grains 138 are arranged on the surface of the column, the rear leg of the nude mouse is pulled by a common sterilized cotton rope, and extends out from the leg fixing bayonet 136 and is fixed on the tie column 137, so that the rear leg of the nude mouse is always in a straight state. The distance from each tie post 137 to the corresponding leg fixing bayonet 136 was set to 5CM in consideration of individual differences of experimental nude mice and versatility in adaptation to each age group. The corresponding row of tie posts 137 and leg fixing notches 136 thus constitutes a rectangular irradiation target area of width 5 CM.

Referring to fig. 23, it is a supporting bracket of the present invention, i.e. a fixing bracket of a flip cover type. The structure adopts the principle that the clamp body is pressed to gather together, the movable folding barrier and the aptamer are limited, realizes the functions of clamping, blocking and braking, and is suitable for contrast irradiation experiments of rats with the body length of 8-10 CM. The device comprises a support main board 18, a magnetic lock head 71, a cover board 72, a cover board groove 73, a mouse osculum hole 74, a flip cover shaft 75, a chamber side board 76, a magnetic lock column 77, a lock rod shaft column 78, a lock rod 79, a chamber front board 132, a support foot locking structure 103, a limb end clamping and positioning structure 106 and a barrier type limiting structure 107. The bracket locking structures 103 are positioned on two sides of the bracket main board 18; four parallel cuboid chamber cavities are arranged on the support main board 18, two sides of each cuboid chamber cavity are chamber side boards 76, the front side is a front board 132, the rear side is a barrier limiting structure 107, a movable covering board 72 is arranged above, and the support main board 18 is arranged below; the upper edge of the chamber side plate 76 is provided with a cover plate groove 73; the center of the front plate 132 is a 74 mouse lip hole; the upper edge of the front plate 132 is provided with a flip shaft 75, and the cover plate 72 is connected with the front plate 132 through the flip shaft 75; three sets of limb end positioning frame structures 106 are equidistantly distributed at the lower end of the fence type limiting structure 107. The magnetic lock post 77 is arranged on the right side of the leftmost cuboid chamber, the lock rod shaft post 78 is arranged on the right side of the rightmost cuboid chamber, one end of the lock rod 79 is connected with the lock rod shaft post 78, the magnetic lock head 71 is connected with the other end of the lock rod 79, and locking of the 72 covering plates of the 4 chambers can be completed.

Referring to fig. 24, it is a matched bracket of the invention, namely a limb positioning type fixed bracket. The structure adopts the principles of pressed gathering of the clamp body, blocked positioning of the latch and axial movement folding and unfolding, realizes the functions of clamping, positioning and limb end folding and unfolding, and is suitable for the irradiation experiment of the rats with the body length of more than 11 CM. The device consists of a fixed table top 16, a support main board 18, a ray absorption bin 21, a biological membrane 30, a biological membrane clamp 31, a central body table 61, a support center 62, a tail included angle adjusting shaft 63, an operation table 70, a support foot locking structure 103, an extremity body splitting shaft structure 105 and an extremity clamping and positioning structure 106. The main body of the invention is a rectangular support main board 18, and support locking structures 103 are positioned at two sides of the support main board 18; the fixed table top 16 is positioned in the middle of the support main board 18 and is divided into an upper layer and a lower layer, the lower layer is a ray absorption bin 21, and a biological membrane 30 and a fixer biological membrane clamp 31 are respectively arranged in the ray absorption bin 21 and mainly used for absorbing irradiated scattered rays, protecting normal tissues and improving the dosage of a target area; the upper strata has acra body of branch body axle construction 105 of lieing in the center to and 5 sets of acra body centre gripping location structures 106 of radial arrangement, and one of them set is connected with tail contained angle degree regulating spindle 63, can rotate on the plane of perpendicular to 61 central body position platform, makes things convenient for the operation of laboratory technician to its caudal cone body. The central body position 61 and the console 70 are located above the acrosome shaft structure 105, wherein the central body position 61 serves to accommodate the mouse body, and the console 70 is designed to be slightly sunk, so that the mouse body is not easy to slide. The 70 operating platform presents a butterfly wing-shaped design, which is convenient for the fixation of the mouse body of an experimenter, the operation of instruments and the (guide) marking of a target area. The center body position 70 is marked with a support center 62.

Example 3 irradiation experiment using the present invention (listing the insert mounting bracket using the experimental box)

(1) (refer to fig. 1-7) preparation phase: opening the valve 173 of the portable gas cylinder 171 to fill the chamber 180 with oxygen as required by the experiment; the heating wire 153 is connected to heat the circuit, and the chamber is kept at a constant temperature. The biological membrane is put into the biological membrane storehouse 21 and fixed by a biological membrane clamp 31, and the function of the biological membrane is to absorb scattered ray. The box cover 162 is opened, and is placed into the 'inserting piece type fixing bracket', and is fixed in the limiting hole 39 on the fixing base plate 40 through the foot rest locking structure 107 arranged on the bracket; the relevant handling instrument is brought into position on the unfolded folding instrument table 167. Finally, a plurality of prepared nude mice are put in, and the box cover is closed. The sealing switch 142 on the box body is rotated, the left sleeve opening 141 and the right sleeve opening 143 are opened, and the hands are stretched into the box.

(2) (refer to fig. 6 and 22) equipment phase: the nude mouse is placed in the chamber 135 of the fixed support, the hind limb or the paw pad implanted with the tumor cell is pulled out, the hind limb or the paw pad is pulled and fixed on the bollard 137, the covering insertion piece 133 is inserted and sealed, and the numbering and the recording are carried out one by one after all. The relation between the center of the bracket and the center 156 of the base plate (generally, the setting centers are overlapped) is adjusted, the A-dimensional adjusting knob 154 and the B-dimensional adjusting knob 160 are rotated to realize the leveling and locking of the bracket main plate 18 of the inserting piece type fixed bracket, and the box is sealed after the inspection is finished.

(3) A transition stage: the irradiation machine room is reached through the whole box body, and a series of microenvironment which cannot be realized before, such as air supply, constant temperature, illumination and the like can be realized due to the portable electrical equipment in the box body in the period. The experiment box is provided with the fixing bracket for locking, can finish fixing and limiting, and does not need to worry about the problems of displacement, pollution, damage and the like in the transferring process and irradiation;

(4) (see fig. 1 and 2) irradiation stage: the fixed box is fixed on the treatment bed, and the irradiation depth is adjusted according to the graduated scale 166 on the box body. Observe through transparent box, set for and shine the field: one is that the field of view is directly divided according to a transparent box body because the target area is fixed; the other is to set the field according to the relative position of the incident center 186 (substrate center 156) and the fixed holder.

(5) And (5) finishing irradiation, returning to a sterile room after the irradiation is finished, and analyzing and comparing at a later date.

Claims

1. A multifunctional experimental box for radiobiology is composed of a box body and a matched fixed support, and is characterized in that the box body is composed of a box back plate structure (109), a box bottom bin structure (110), a three-dimensional fixed base plate structure (111), an aseptic looping structure (112), a box chamber (180), a box side plate (179) and an incident central point (186); the box rear plate structure (109) forms the rear wall of the box chamber (180), two side walls of the box chamber (180) are formed by box side plates (179), the front wall of the box chamber (180) is of a sterile looping structure (112), the three-dimensional fixed base plate structure (111) forms the bottom of the box chamber (180), and the box bottom bin structure (110) is arranged below the three-dimensional fixed base plate structure (111); the supporting fixed bolster includes: the fixing device comprises an in vitro cell experiment fixing bracket, an inserting piece type fixing bracket, a flip type fixing bracket and a limb end positioning type fixing bracket;

the box rear plate structure (109) consists of a box cover (162), a box cover shaft (163), an ultraviolet disinfection lamp tube (164), an illumination lamp tube (165), a graduated scale (166), a folding instrument table (167), a first folding shaft (168), a pull wire (169), a 220V power supply jack (170), a table top sucker (187), a rear plate (188) and a top plate (189); the box cover (162) is connected to one side of the top plate (189) through a box cover shaft (163); the other edge of the top plate (189) intersects and is perpendicular to the back plate (188); the ultraviolet disinfection lamp tube (164) and the lighting lamp tube (165) are arranged below the top plate (189) in parallel; a graduated scale (166) and a 220V power supply jack (170) which are vertically arranged are respectively arranged at one side edge and below the rear plate (188); a folding instrument table (167) is designed at the middle position of the left side of the rear plate (188), and the folding instrument table (167) is respectively connected with a first folding shaft (168) and a pull wire (169) on the rear plate (188); a magnetic block is arranged in the middle of the free edge of the long shaft of the folding instrument table (167) and is matched with the table top sucker (187) on the rear plate (188); the box bottom bin structure (110) is composed of a biological membrane (30), a biological membrane clamp (31), a portable gas tank (171), a gas bottle nozzle (172), a valve (173), a filter bottle gas outlet nozzle (174), a filter bottle (175), a filter bottle gas inlet nozzle (176), an air conditioning equipment bin (177), a first ray absorption bin (178), an electric bin (181), a No. I storage battery (182), a No. II storage battery (183) and a transformer (184); the device comprises an electric bin (181), a first ray absorption bin (178) and an air conditioning equipment bin (177), wherein the three bins are arranged at the bottom in parallel; two biomembrane clamps (31) are respectively arranged on two side walls in the first ray absorption bin (178) to clamp the biomembrane (30); the air conditioning equipment bin (177) is positioned on one side of the first ray absorption bin (178) and is internally provided with two portable gas tanks (171) and filter bottles (175), the portable gas tanks (171) are provided with gas bottle nozzles (172), gas in and out of the gas bottle nozzles (172) are controlled by valves (173), and the filter bottles (175) are provided with filter bottle air outlet nozzles (174) and filter bottle air inlet nozzles (176); the electric bin (181) is positioned at the other side of the first ray absorption bin (178), and a No. I storage battery (182), a No. II storage battery (183) and a transformer (184) are sequentially arranged in the electric bin;

the three-dimensional fixed substrate structure (111) consists of a limiting hole (39), a fixed substrate (40), a bottom plate (140), an electric heating wire (153), an A-dimensional adjusting knob (154), an A-dimensional adjusting shaft (155), a substrate center (156), an A-dimensional level meter (157), a dimensional partition plate (158), a B-dimensional adjusting shaft (159), a B-dimensional adjusting knob (160), a B-dimensional level meter (161) and a gas outlet (185); the bottom plate (140) is rectangular, the A-dimension adjusting shaft (155) and the B-dimension adjusting shaft (159) are respectively positioned on two symmetrical axes of the bottom plate (140), wherein the length of the symmetrical axis corresponding to the A-dimension adjusting shaft (155) is smaller than the length of the symmetrical axis corresponding to the B-dimension adjusting shaft (159); a dimension plate (158) is arranged between the dimension A adjusting shaft (155) and the dimension B adjusting shaft (159); two ends of the A-dimensional adjusting shaft (155) are respectively provided with an A-dimensional adjusting knob (154), and the A-dimensional adjusting knobs (154) are semi-cylindrical and are arranged on the bottom plate (140); b dimension adjusting knobs (160) are arranged at two ends of the B dimension adjusting shaft (159), and the B dimension adjusting knobs (160) are cylindrical and are arranged outside the bottom plate (140); the fixed substrate (40) is positioned on the A-dimensional adjusting shaft (155) and the B-dimensional adjusting shaft (159), the center of the fixed substrate (40) is carved with a substrate center (156), and two sides of the substrate center (156) of the fixed substrate (40) are respectively provided with a beam of electric heating wire (153); a plurality of limiting holes (39) are equidistantly distributed on the fixed base plate (40); the long axis of the A-dimensional gradienter (157) is parallel to the B-dimensional adjusting axis, the long axis of the B-dimensional gradienter (161) is parallel to the A-dimensional adjusting axis (155), and the A-dimensional gradienter (157) and the B-dimensional gradienter (161) are respectively arranged on two corners of the fixed substrate (40); the gas outlets (185) are located on the corners of the bottom plate (140);

the sterile sleeve opening structure (112) consists of an inner plate (139), a bottom plate (140), a right sleeve opening (141), a sealing switch (142), a left sleeve opening (143), an outer plate (144), a left sealing cover (145), a gear (146), a left rail tooth (147), a right rail groove (148), a right sealing cover (149), a gear shaft (150), a right rail tooth (151) and a left rail groove (152); a right sleeve opening (141) and a left sleeve opening (143) are formed in the upper opening of the inner plate (139), a right rail groove (148) and a left rail groove (152) are obliquely formed between the right sleeve opening (141) and the left sleeve opening (143), left rail teeth (147) run in the right rail groove (148), right rail teeth (151) run in the left rail groove (152), a gear (146) is meshed between the left rail teeth (147) and the right rail teeth (151), a gear shaft (150) is vertical to the inner plate (139) and is connected with the gear (146), a left sealing cover (145) is connected with the port of the left rail teeth (147) and is parallel to the inner plate (139), and a right sealing cover (149) is connected with the port of the right rail teeth (151) and is parallel to the inner plate (139); the outer plate (144) is provided with a right sleeve opening (141) and a left sleeve opening (143) and corresponds to the right sleeve opening (141) and the left sleeve opening (143) on the inner plate (139), and a gear shaft (150) which is vertical to the inner plate (139) is connected with a sealing switch (142) on the outer plate (144);

the in-vitro cell experiment fixing support consists of a fixing table board (16), a support main board (18), a second ray absorption bin (21), a biological membrane (30), a biological membrane clamp (31), a fixing base plate (40), a left limiting structure, a right limiting structure (100), a front limiting structure, a rear limiting structure (101), a left auxiliary limiting structure, a right auxiliary limiting structure (102) and a support foot locking structure (103); the fixed table top (16) is positioned in the middle of the support main board (18), the fixed table top (16) is divided into an upper layer and a lower layer, the lower layer is a second ray absorption bin (21), a biological membrane (30) and a biological membrane clamp (31) are arranged in the second ray absorption bin (21), and the biological membrane (30) is fixed through the biological membrane clamp (31); the upper layer is provided with a left-right limiting structure (100), a front-back limiting structure (101) and a left-right auxiliary limiting structure (102), wherein two sets of front-back limiting structures (101) are respectively embedded at the edges of the front side and the back side of the upper layer of the fixed table top (16); a left auxiliary limiting structure and a right auxiliary limiting structure (102) are embedded in the center of the upper layer of the fixed table top (16), and the two left limiting structures and the right limiting structures (100) are arranged on the long shaft of the upper layer of the fixed table top and are positioned at two sides of the left auxiliary limiting structure and the right auxiliary limiting structure (102);

the left and right limiting structure (100) is composed of a cornice blocking needle (1), a main limiting clamping groove (2), an arc-shaped limiting clamping block (3), a main limiting base (4), a main limiting spring (5), a height limiting spring (6), a height limiting adjusting handle (7), a height limiting clamping groove (8), a height limiting clamping body (9), a cornice limiting spring (10), a cornice limiting clamping groove (11), a cornice limiting base (12) and a cornice limiting clamping block (13); a main limiting spring (5) and a main limiting base (4) run by the main limiting clamping groove (2), wherein one end of the main limiting spring (5) is connected with the main limiting base (4), and the other end of the main limiting spring is fixed at the far end of the main limiting clamping groove (2); the main limiting base (4) is connected with an arc-shaped limiting clamping block (3), the middle opening of the clamping block is provided with a height limiting clamping groove (8), and a height limiting spring (6), a height limiting adjusting handle (7) and a height limiting clamping body (9) are sequentially arranged in the clamping block in a walking way; an eaves limiting clamping groove (11) is formed in the eaves limiting clamping block (13), an eaves limiting base (12) and an eaves limiting spring (10) run in the eaves limiting clamping groove (11), one end of the eaves limiting spring (10) is connected with the far end of the eaves limiting clamping groove (11), and the other end of the eaves limiting spring is connected with the eaves limiting base (12); the cornice limiting clamping groove (11) is internally provided with a cornice limiting base (12), and the cornice limiting base (12) is connected with the height limiting clamping body (9); the near end of the cornice limiting clamping block (13) is provided with a cornice blocking needle (1); the force application direction of the main limiting spring (5) is from the far end to the near end of the main limiting clamping groove (2), and the force application direction of the height limiting spring (6) is from top to bottom; the force application direction of the cornice limiting spring (10) is from the near end to the far end of the cornice limiting clamping groove (11);

the front and rear limiting structures (101) are composed of cornice blocking needles (1), main limiting clamping grooves (2), main limiting bases (4), main limiting springs (5), height limiting springs (6), height limiting adjusting handles (7), height limiting clamping grooves (8), height limiting clamping bodies (9), cornice limiting springs (10), cornice limiting clamping grooves (11), cornice limiting bases (12), cornice limiting clamping blocks (13) and strip-shaped limiting clamping blocks (14); a main limiting spring (5) and a main limiting base (4) run in the main limiting clamping groove (2), wherein one end of the main limiting spring (5) is connected with the main limiting base (4), and the other end of the main limiting spring is fixed at the far end of the main limiting clamping groove (2); the main limiting base (4) is connected with a strip-shaped limiting clamping block (14), the middle opening of the strip-shaped limiting clamping block (14) is provided with a height limiting clamping groove (8), and a height limiting spring (6), a height limiting adjusting handle (7) and a height limiting clamping body (9) sequentially run in the height limiting clamping groove; an eaves limiting clamping groove (11) is formed in the eaves limiting clamping block (13), an eaves limiting base (12) and an eaves limiting spring (10) run in the eaves limiting clamping groove (11), one end of the eaves limiting spring (10) is connected with the far end of the eaves limiting clamping groove (11), and the other end of the eaves limiting spring is connected with the eaves limiting base (12); the cornice limiting clamping groove (11) is connected with the height limiting clamping body (9) through a cornice limiting base (12); the near end of the cornice limiting clamping block (13) is provided with a cornice blocking needle (1); the force application direction of the main limiting spring (5) is from the far end to the near end of the main limiting clamping groove (2), and the force application direction of the height limiting spring (6) is from the top to the bottom; the force application direction of the cornice limiting spring (10) is from the near end to the far end of the cornice limiting clamping groove (11);

the left and right auxiliary limiting structures (102) are composed of cornice blocking needles (1), height limiting springs (6), height limiting adjusting handles (7), height limiting clamping grooves (8), height limiting clamping bodies (9), cornice limiting springs (10), cornice limiting clamping grooves (11), cornice limiting bases (12), cornice limiting clamping blocks (13), strip-shaped limiting clamping blocks (14), second folding shafts (15) and folding bins (17); the second folding shaft (15) is fixed in the folding bin (17); one end of the strip-shaped limiting clamping block (14) is connected to the second folding shaft (15), and the other end is free; a height limiting clamping groove (8) is formed in the strip-shaped limiting clamping block (14); a height limiting spring (6), a height limiting adjusting handle (7) and a height limiting clamping body (9) are sequentially arranged in the height limiting clamping groove (8) from bottom to top; the top end of the height limiting clamp body (9) is connected with an eaves limiting base (12); the cornice limiting clamping block (13) is provided with a cornice limiting clamping groove (11), a cornice limiting base (12) and a cornice limiting spring (10) run in the cornice limiting clamping groove (11), and one end of the cornice limiting spring (10) is connected with the cornice limiting base (12) and the far end of the cornice limiting clamping groove (11); the cornice limiting clamping groove (11) is connected with the height limiting clamping body (9) through a cornice limiting base (12); the near end of the cornice limiting clamping block (13) is provided with a cornice blocking needle (1); the force application direction of the height limiting spring (6) is from top to bottom; the force application direction of the cornice limiting spring (10) is from the near end to the far end of the cornice limiting clamping groove (11);

the insert type fixing support consists of a support main board (18), a mouse osculum hole (74), a support foot locking structure (103), a covering insert (133), a groove (134), a chamber cavity (135), a leg fixing bayonet (136), a stud (137) and friction grains (138); the support comprises a support main board (18), a chamber (135) arranged on the support main board (18), mouse-lip holes (74) arranged on the front wall of the chamber (135), grooves (134) arranged on the upper edges of the left wall and the right wall of the chamber (135), covering insertion sheets (133) inserted into the grooves (134), leg fixing bayonets (136) arranged on the rear wall of the chamber (135), tie columns (137) arranged at positions corresponding to the leg fixing bayonets (136), friction grains (138) arranged on the tie columns (137), and support foot locking structures (103) arranged on two sides of the support main board (18);

the flip type fixing support consists of a support main board (18), a magnetic lock head (71), a cover plate (72), a cover plate groove (73), a mouse lip part hole (74), a flip cover shaft (75), a chamber side board (76), a magnetic lock column (77), a lock rod shaft column (78), a lock rod (79), a chamber front board (132), a support foot locking structure (103), a limb end positioning clamp structure (106) and a barrier type limiting structure (107); the bracket foot locking structures (103) are positioned on two sides of the bracket main board (18); four parallel cuboid chamber cavities are arranged on a support main board (18), two sides of each cuboid chamber cavity are chamber side boards (76), the front side is a chamber front board (132), the rear side is a barrier type limiting structure (107), a movable covering board (72) is arranged above the cuboid chamber cavity, and the support main board (18) is arranged below the cuboid chamber cavity; a cover plate groove (73) is arranged on the upper edge of the chamber side plate (76); a rat-mouth hole (74) is formed in the center of the chamber front plate (132); a flip shaft (75) is arranged on the upper edge of the chamber front plate (132), and the cover plate (72) is connected with the chamber front plate (132) through the flip shaft (75); three sets of limb end positioning clamp structures (106) are equidistantly distributed at the lower end of the fence type limiting structure (107); a magnetic lock column (77) is arranged on the right side of the leftmost cuboid chamber, a lock rod shaft column (78) is arranged on the right side of the rightmost cuboid chamber, one end of a lock rod (79) is connected with the lock rod shaft column (78), and the other end of the lock rod is connected with a magnetic lock head (71); the fence type limiting structure (107) is composed of a middle positioning left knob (80), a middle positioning right knob (81), a third folding shaft (82), a lateral positioning right knob (83), an upper inner guide rail (84), an upper guide groove (85), an upper outer guide rail (86), a right frame (87), a lateral right clamping column (88), a lower inner guide rail (89), a lower guide groove (90), a lower outer guide rail (91), a guide wheel (92), a middle right clamping column (93), a middle limiting stop (94), a middle left clamping column (95), a folding clamping sheet (96), a lateral left clamping column (97), a left frame (98), a guide rail shaft (99), a guide rail rod cap (130) and a lateral positioning left knob (131); the upper inner guide rail (84) is parallel to the upper outer guide rail (86), and both ends of the upper inner guide rail are respectively connected with the upper parts of the right frame (87) and the left frame (98); the lower inner guide rail (89) is parallel to the lower outer guide rail (91), and two ends of the lower inner guide rail are respectively connected with the lower parts of the right frame (87) and the left frame (98); an upper guide groove (85) is formed between the upper inner guide rail (84) and the upper outer guide rail (86), a lower guide groove (90) is formed between the lower inner guide rail (89) and the lower outer guide rail (91), the upper guide groove (85) and the lower guide groove (90) are parallel and opposite, a middle limit stop (94) is arranged in the middle of the lower guide groove (90) to divide the lower guide groove (90) into a left part and a right part; a side left clamping column (97), three guide rail shafts (99) and a middle left clamping column (95) are sequentially arranged inside the left side of the upper guide groove (85); a folding card (96), a third folding shaft (82) and the folding card (96) are sequentially connected among the lateral left clamping column (97), the three guide rail shafts (99) and the middle left clamping column (95); the upper end of the side left clamping column (97) is connected with a side positioning left knob (131), the upper ends of the three guide rail shafts (99) are respectively connected with a guide rail rod cap (130), and the upper end of the middle left clamping column (95) is connected with a middle positioning left knob (80); the lower ends of the side left clamping column (97), the three guide rail shafts (99) and the middle left clamping column (95) are respectively connected with a guide wheel (92) and run at the left side part of the lower guide groove (90); a side right clamping column (88), three guide rail shafts (99) and a middle right clamping column (93) are sequentially arranged in the right side of the upper guide groove (85); a folding card (96), a third folding shaft (82) and a folding card (96) are sequentially connected among the side right clamping column (88), the three guide rail shafts (99) and the middle right clamping column (93); the upper end of the side right clamping column (88) is connected with a side positioning right knob (83), the upper ends of the three guide rail shafts (99) are connected with a guide rail rod cap (130), and the upper end of the middle right clamping column (93) is connected with a middle positioning right knob (81); the lower ends of the side right clamping column (88), the three guide rail shafts (99) and the middle right clamping column (93) are respectively connected with a guide wheel (92) and run at the right side part of the lower guide groove (90);

the limb end positioning type fixing support consists of a fixing table top (16), a support main board (18), a second ray absorption bin (21), a biological membrane (30), a biological membrane clamp (31), a central body table (61), a support center (62), a tail included angle adjusting shaft (63), an operation table (70), a support foot locking structure (103), a limb end split body shaft structure (105) and a limb end positioning clamp structure (106); the bracket foot locking structures (103) are positioned on two sides of the bracket main board (18); the fixed table top (16) is positioned in the middle of the support main board (18), the fixed table top (16) is divided into an upper layer and a lower layer, the lower layer is a second ray absorption bin (21), and a biological membrane (30) and a fixer biological membrane clamp (31) are respectively arranged in the second ray absorption bin (21); the upper layer is provided with a limb end split shaft structure (105) positioned in the center and 5 sets of limb end positioning clamp structures (106) arranged in a radial manner, wherein one set is connected with a tail included angle adjusting shaft (63); the central body position table (61) and the operating table (70) are positioned above the limb end split shaft structure (105), and the support center (62) is marked on the central body position table (61);

the limb-end split shaft structure (105) is composed of a limb-end angle adjusting area (64), a limb-end angle tightening screw (65), a limb-end split shaft (66), a limb-end split tightening sheet (67), a shaft bin (68) and a limb-end split rod (69); the central part of the operating table (70) is sunken to form a shaft bin (68), and the limb body shaft (66) is positioned in the center of the shaft bin (68); four limb end split rods (69) radiate from the center of the shaft, run in limb end split angle adjusting areas (64) around the shaft bin (68), and are connected with limb end positioning clamp structures (106) outwards; the extremity angle-dividing elastic screw (65) is designed on the extremity body shaft (66), and the extremity body elastic sheet (67) is distributed on the shaft.

2. The multifunctional radiobiological laboratory box according to claim 1, characterized in that the supporting leg locking structure (103) is composed of a handle (23), a handle column (24), an embedded column (26), a left bolt (27) and a right bolt (28), the handle column (24) is installed on the supporting main board (18), the tail end of the handle column is connected with the embedded column (26), and the top ends of the two handle columns (24) are connected through the handle (23); a tongue rod column (38) runs in the handle column (24), and a push rod groove (37) and a tongue rod groove (33) run in the tongue rod column (38); the bottom of the tongue bar groove (33) is provided with two opposite wedge-shaped springs (29), and the top of the tongue bar groove is provided with a tongue bar shaft (43); two tongue rods (34) run in the tongue rod groove (33), and the tongue rods (34) run along the wall of the tongue rod groove (33) after being sent out by a tongue rod shaft (43) and are dissociated from the lower end opening of the embedded column (26); the tongue rods (34) are provided with wedge-shaped parts (32) corresponding to the wedge-shaped part springs (29), and the tail ends of the two tongue rods (34) are respectively connected with the left bolt (27) and the right bolt (28); a push ball (36) is clamped between the two wedge-shaped parts (32); a push seat (44) is arranged at the top end in the push rod groove (37), and the upper part of the push seat (44) is connected with a push seat spring (42); the handle (23) is connected with the linkage rod (41), and the side part of the push seat (44) is connected with the linkage rod (41); the bottom surface of the handle (23) is provided with an opening and is provided with an unlocking button (19), and the unlocking button (19) is connected with the linkage rod (41).

3. The multifunctional radiobiological experimental box according to claim 1, characterized in that the extremity positioning clip structure (106) is composed of a positioning slot (57), a positioning spring (58), a wedge-shaped positioning latch (59), a positioning guide rail (60), and a positioning clip structure (104), wherein the positioning clip structure (104) is composed of a left crescent clip (45), a right crescent clip (46), two crescent clip shafts (47), a clip slot (48), a clip base (49), a clip spring (50), a clip main handle (51), two clip handle shafts (52), a positioning base (53), a clip handle spring (54), a positioning clip handle (55), and a clip sheath (56); a positioning clamping groove (57) is formed in the positioning guide rail (60); wedge-shaped positioning latch teeth (59) are distributed on the inner wall of one side of the positioning clamping groove (57) at equal intervals; the wedge-shaped positioning latch (59) is divided into a thick head and a thin head, and the thick head faces to the far end; the positioning spring (58) is arranged in the positioning clamping groove (57), one end of the positioning spring is fixed at the far end of the positioning clamping groove (57), and the other end of the positioning spring is connected with the positioning base (53) of the positioning shrinkage clamp structure (104); the positioning clamp handle (55) and the shrinkage clamp main handle (51) are fixed on the positioning base (53) through respective clamp handle shafts (52), and a clamp handle spring (54) is arranged between the positioning clamp handle (55) and the shrinkage clamp main handle (51); the bottom end of the shrinkage clamp outer sleeve (56) is connected with a shrinkage clamp main handle (51), a shrinkage clamp groove (48) is arranged in an opening at the upper end, a shrinkage clamp spring (50) is arranged in the shrinkage clamp groove (48), and the upper end of the shrinkage clamp spring (50) is connected with a shrinkage clamp base (49); the left crescent clamp (45) and the right crescent clamp (46) are connected with a shrinking clamp base (49) through a crescent clamp shaft (47).