CN104111327A - 木质纤维检测用具 - Google Patents
木质纤维检测用具 Download PDFInfo
- Publication number
- CN104111327A CN104111327A CN201310251514.XA CN201310251514A CN104111327A CN 104111327 A CN104111327 A CN 104111327A CN 201310251514 A CN201310251514 A CN 201310251514A CN 104111327 A CN104111327 A CN 104111327A
- Authority
- CN
- China
- Prior art keywords
- wood fibre
- base material
- detection
- detects
- wood fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920002522 Wood fibre Polymers 0.000 title claims abstract description 75
- 238000001514 detection method Methods 0.000 title claims abstract description 54
- 239000002025 wood fiber Substances 0.000 title abstract 9
- 239000000463 material Substances 0.000 claims abstract description 51
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 36
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 230000009471 action Effects 0.000 claims abstract description 11
- 235000010585 Ammi visnaga Nutrition 0.000 claims description 24
- 244000153158 Ammi visnaga Species 0.000 claims description 24
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 19
- 239000008103 glucose Substances 0.000 claims description 19
- 238000013098 chemical test method Methods 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 13
- 239000008280 blood Substances 0.000 claims description 8
- 210000004369 blood Anatomy 0.000 claims description 8
- 210000003296 saliva Anatomy 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 6
- 239000001257 hydrogen Substances 0.000 claims description 5
- 229910052739 hydrogen Inorganic materials 0.000 claims description 5
- 238000004381 surface treatment Methods 0.000 claims description 5
- 210000002700 urine Anatomy 0.000 claims description 5
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 4
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 4
- 239000011425 bamboo Substances 0.000 claims description 4
- -1 nitrite anions Chemical class 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 230000005661 hydrophobic surface Effects 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 12
- 230000036541 health Effects 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 230000006806 disease prevention Effects 0.000 abstract 1
- 238000012423 maintenance Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 239000002585 base Substances 0.000 description 32
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 20
- 238000012360 testing method Methods 0.000 description 19
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 14
- 239000000243 solution Substances 0.000 description 10
- 235000012000 cholesterol Nutrition 0.000 description 9
- 108010015776 Glucose oxidase Proteins 0.000 description 8
- 239000004366 Glucose oxidase Substances 0.000 description 8
- 229940116332 glucose oxidase Drugs 0.000 description 8
- 235000019420 glucose oxidase Nutrition 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 239000004205 dimethyl polysiloxane Substances 0.000 description 5
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 5
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 5
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 4
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 4
- 241001330002 Bambuseae Species 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- FDDDEECHVMSUSB-UHFFFAOYSA-N sulfanilamide Chemical compound NC1=CC=C(S(N)(=O)=O)C=C1 FDDDEECHVMSUSB-UHFFFAOYSA-N 0.000 description 3
- 108010089254 Cholesterol oxidase Proteins 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 239000005445 natural material Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 229940124530 sulfonamide Drugs 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- HVYWMOMLDIMFJA-UHFFFAOYSA-N 3-cholesterol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 HVYWMOMLDIMFJA-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000544043 Blyxa aubertii Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- WEBZERCQZACOAD-UHFFFAOYSA-N N'-naphthalen-1-ylethane-1,2-diamine Chemical compound C1(=CC=CC2=CC=CC=C12)NCCN.C1(=CC=CC2=CC=CC=C12)NCCN WEBZERCQZACOAD-UHFFFAOYSA-N 0.000 description 1
- NULAJYZBOLVQPQ-UHFFFAOYSA-N N-(1-naphthyl)ethylenediamine Chemical compound C1=CC=C2C(NCCN)=CC=CC2=C1 NULAJYZBOLVQPQ-UHFFFAOYSA-N 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical compound ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- NHDHVHZZCFYRSB-UHFFFAOYSA-N pyriproxyfen Chemical compound C=1C=CC=NC=1OC(C)COC(C=C1)=CC=C1OC1=CC=CC=C1 NHDHVHZZCFYRSB-UHFFFAOYSA-N 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000021108 sauerkraut Nutrition 0.000 description 1
- 229920005573 silicon-containing polymer Polymers 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/528—Atypical element structures, e.g. gloves, rods, tampons, toilet paper
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
- G01N31/227—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators for nitrates or nitrites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Diabetes (AREA)
- Inorganic Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
木质纤维检测用具包含:一木质纤维基材;以及至少一检测试剂。检测试剂吸附/涂布于木质纤维基材的木质纤维组织,其中当木质纤维基材与一液态检体或一含有液体的检体接触时,检体透过毛细作用与木质纤维基材的检测试剂进行反应,以达成检体检测的目的。本发明的木质纤维检测用具具有包括天然材质、价格便宜、加工方便等优势,目的是使木质纤维基材在传统利用之外,让使用者不需花费额外的时间/金钱即可达成疾病预防检测,进而成为日常健康维护的平台。
Description
技术领域
本发明系关于检测用具,特别是关于利用毛细作用进行检测的木质纤维检测用具。
背景技术
随着民众的健康意识逐渐提高,居家自我检测的概念已渐渐盛行。所谓居家自我检测意指民众可在家方便随时检测,通常可利用检测试剂的颜色转变,在简便仪器或不需仪器肉眼判读的条件下,可立即观察有无疾病的征兆。此外当色差变大时,可再到医院进一步详细检查。因此,居家自我检测具有方便即时及节省支出的优势。
在居家自我检测方面,检测试纸为经常使用的检测用品。检测试纸的优点为:操作简单、容易判读及方便携带。检测试纸的检测原理主要是利用酵素与溶液中的特定物质进行催化的生化反应,产生颜色或其他性质改变,以量测物质存在与否的定性反应,以及物质浓度的半定量反应。
举例而言,居家使用型的血糖或尿糖检验试纸的原理为「葡萄糖氧化酶(glucose oxidase)法」,利用葡萄糖氧化酶与葡萄糖的间的专一性催化反应使试纸变色,对他种还原性的物质则不起作用。利用酵素的技术在试纸上涂上一种葡萄糖氧化酶,当葡萄糖氧化酶与血液中的葡萄糖反应后,会使得试纸颜色产生变化,而使用者可根据试纸的颜色与颜色表比对,即可判读血糖浓度的高低。这种简易的血糖量测方法,造福许多糖尿病的患者,使其可随时对病情作监控,不仅改善生活品质,同时也扩大了医疗照护的范围。
惟检测试纸仍需要特别携带,因此检测试纸的方便性仍有改良的空间。因此,发展一种检测平台,其具有试纸的优点,并拥有与日常用品匹配的便利性,为当前努力的目标。
发明内容
本发明的目的之一系开发一种检测平台,其具有试纸的优点,并可拥有与日常用品匹配的便利性。
依据本发明的一实施例,一种木质纤维检测用具包含:一木质纤维基材;以及至少一检测试剂。检测试剂吸附/涂布于木质纤维基材的木质纤维组织,其中当木质纤维基材与一液态检体或一含有液体的检体)接触时,检体透过毛细作用与木质纤维基材的检测试剂进行反应,以达成检体检测的目的。
本发明的木质纤维基材包括一搅拌棒、一木筷或一牙签。
以下藉由具体实施例配合所附的图式详加说明,当更容易了解本发明的目的、技术内容、特点及其所达成的功效。
附图说明
图1为示意图显示本发明的木质纤维检测用具。
图2a至2c为实验数据显示本发明的一实施例的一检测用牙签用以测量葡萄糖。
图3a至3c为实验数据显示本发明的一实施例的一检测用牙签用以测量胆固醇。
图4为照片显示本发明的一实施例的一检测用牙签用以测量亚硝酸盐。
图5为照片显示本发明的一实施例的一表面处理的搅拌棒。
图6为照片显示本发明的一实施例的一检测用搅拌棒用以测量亚硝酸盐。
【符号说明】
1 木质纤维基材
11 凹槽
2 检测试剂
具体实施方式
本发明系关于一种木质纤维检测用具,其主要元件为木质纤维基材及检测试剂。木质纤维或可称为木质化组织,其指的是维管形成层向内的发展出植物组织的统称。此外,木质纤维通常取自乔木和灌木所形成的木质化组织。木质纤维具有强韧的机械结构并相对耐酸碱,因此可较滤纸具有结构强度方面的优势。
木质纤维基材的形状、大小原则上并未限制,因此可依检测标的来进行设计。常见的木质纤维基材的形状包含但不限于圆柱、长方体、板状等。
请参照图1,其为示意图显示本发明的木质纤维检测用具,其中本发明的木质纤维基材1包含凹槽11。一般而言,凹槽的形状、大小并未特别限制,可视检测需求而设置。其中应注明的是,图1实例中凹槽11的形状仅为示意而非用以限制。一般而言,凹槽11的形状可包含但不限于长方体、正方体、圆柱、半球体、V型或上述之外其余的形状组合。凹槽11的位置亦可视不同检测的需求而做弹性设置。
在一实施例中凹槽11可设置为检测区,因此可将检测试剂2设置于凹槽11,以便进行检测。在图1中检测试剂2设置于凹槽11的底部。但应可了解检测试剂亦可设置于凹槽的单侧、底面、双侧或其他组合。在一实施例中,可将液体样本滴至检测区以直接与检测试剂反应,进而检测液体样本。
应注明的是,在另一实施例中,可利用木质纤维基材1的一端作为与液体样本的接触区,再经由木质纤维基材1的内部输水孔道(图中未示)所具有的毛细作用进行欲检测标的物的运送到达作为检测区的凹槽11,进而避免木质纤维基材1表面输水孔道因加工过程的物理性破坏而造成量测误差,甚至达到控制液体样本的流速的目的。
此外可对木质纤维基材进行表面处理以增加木质纤维的稳定性,表面处理例如但不限于疏水处理,疏水试剂包含但不限于PDMS(聚二甲基硅氧烷,Polydimethylsiloxane)。
此外应注明的是,多重检测可以多重定性或是定量;详言之,多重检测可包含多样检测标的或是单一检测标的多重浓度的检测,以达检测的多样性。
在一实施例中,木质纤维基材可采用日常生活用品,其实施例包含但不限于搅拌棒、木筷或牙签。
其中本发明的一实施例为牙签。牙签的一般用途主要是用来清洁牙缝中的食物,其材质为木质纤维,台湾较常见者为竹制材料。竹类植物的杆多为木质,属灌木或乔木状,由于生长快速,因此有价格的优势。
牙签的优势包括天然材质、价格便宜、加工方便等优点。牙签取得方便并常用于餐后维护口腔卫生,本发明的目的之一即是让使用者在使用牙签清洁牙缝时,一并完成疾病检测,使得使用者不需花费额外的时间即可达成疾病检测。
本发明的原理为利用木质纤维所具有的毛细作用及利用吸附于木质纤维基材纤维组织的检测试剂,使得木质纤维基材成为疾病检测平台。当木质纤维基材的一端与一液态检体或一含有液体的检体接触时,藉由毛细作用将标的检体吸附至木质纤维基材的纤维组织,并与检测试剂反应,以达成检测检体的作用。
检测试剂吸附于木质纤维基材的方法系藉由将木质纤维基材浸泡于检测试剂溶液而完成,藉由木质纤维基材的毛细作用及植物纤维的维管束组织,使得检测试剂吸附于木质纤维基材。其中将木质纤维基材浸泡于检测试剂溶液后,可进行一干燥步骤,以制备可进行检测的木质纤维基材。
本发明的检测用木质纤维基材可以利用呈色(colorimetric)反应或是荧光进行检测,其中检测试剂包含一呈色剂或一荧光试剂。检测试剂的呈色剂或荧光试剂可依检测标的不同而做适应性改变。
在本发明的一实施例中,检测试剂包含一酵素。如前所述,可利用酵素与溶液中的物质反应,产生颜色或其他性质改变,以量测物质存在与否的定性反应,以及物质浓度的定量反应。举例而言,测量「葡萄糖氧化酶(glucose oxidase)法」,则是利用葡萄糖氧化酶与葡萄糖的间的专一性催化作用使试纸变色。
检体举例但不限于一受试者的唾液、血液、尿液或其他体液。如前所述检体藉由毛细作用吸附至木质纤维基材的纤维组织,并与检测试剂反应以达成检测检体的作用。此外亦可将固体检测物悬浮于液体溶剂之中,待标的检体溶出于溶剂之中,再利用毛细作用进行上述的检测方式。
其中应注明的是本发明的木质纤维基材,常用于进食或是喝饮料,例如:餐后维护口腔卫生,因此一较佳实施例为检测受试者的唾液。而唾液检测的优点包括:唾液检验为非侵入性、采集方便、成本不高、感染风险低、病人不须依赖医护人员即可自行采集、自行检验。
承上述,本发明的检测用木质纤维基材可用于检测唾液、血液、尿液或其他体液的相关生化性质,举例而言但不限于检测葡萄糖、亚硝酸根、酸碱度。以下仅为实施例说明检测原理及应用。
1.亚硝酸盐检测
食物中如有硝酸离子,口水中的消化酵素作用后会转变为亚硝胺,其已被医界证实为一致癌物。此外含有大量亚硝酸盐的饮水、蔬菜、粮食、鱼、肉制品、渍酸菜等经人食用后,大量亚硝酸盐可使人直接中毒。目前已有利用化学呈色反应的亚硝酸检测试纸,因此食物中亚硝酸检测亦为适宜的检测标的。
2.葡萄糖检测
血糖或尿糖的测量原理已如前述,其可使病患能够随时对自己的病情作监控。
3.酸碱度检测
在检测酸碱度方面,石蕊试纸是常用来测量溶液的酸碱度的指示用具。石蕊试纸是将纸张浸于含石蕊试剂的溶液中制成。石蕊试剂在酸性液中呈红色,在碱性液中呈蓝色。故可利用前述性质制备用以检测酸碱性的木质纤维基材。
以下通过具体实施例配合附图详加说明,可更容易了解本发明的目的、技术内容、特点及所达成的功效,并据以实施,但不能以此限定本发明的保护范围。
牙签作为葡萄糖测量用具利用牙签作为葡萄糖测量用具的制备及检测方法如下:(1)将牙签浸泡于330μl的葡萄糖检测试剂中:含Glucose oxidase75units/mL,HRP15units/mL,荧光染剂10-acetyl-3,7-dihydroxyphenoxazine300μM,上述药品皆来自Sigma-Aldrich。(2)浸泡五分钟。(3)取出干燥2小时。(4)干燥后即可于葡萄糖检测用。图2显示使用牙签做为检测缓冲液系统中不同浓度的葡萄糖的吸附结果。其中图2a为校正曲线(Calibration curve)显示荧光平均强度与葡萄糖浓度的对应关系(N=20)。图2b为图像显示缓冲液系统中2.5mM和500mM葡萄糖的牙签荧光检测图像。图2c为具有相同葡萄糖浓度的不同试验组之间的差异(每组N=5),其中在不同的葡萄糖浓度(2.5,5,10,50和500mM)测试组之间的平均变化分别为0.633,0.336,0.118,0.034和0.026。
牙签作为胆固醇测量用具利用牙签作为胆固醇测量用具的制备及检测方法如下:(1)将牙签浸泡于330μl的胆固醇检测试剂中:含胆固醇氧化酶(Cholesterol oxidase)2units/mL,HRP2units/mL,荧光染剂10-acetyl-3,7-dihydroxyphenoxazine300μM,以上药品皆来自Sigma-Aldrich。(2)浸泡五分钟。(3)取出干燥0.5小时。(4)干燥后即可于胆固醇检测用。图3显示使用牙签做为检测缓冲液系统中不同浓度的胆固醇的吸附结果。图3a为校正曲线显示显示荧光平均强度与胆固醇浓度的对应关系(N=15)。图3b为图像显示缓冲液系统中1.5mg/mL和4mg/mL胆固醇的牙签荧光检测图像。图3c数据显示具有相同胆固醇浓度的不同试验组之间的差异(每组N=5),其中在不同的胆固醇浓度(1.5,2,2.5,3,和4mg/mL)测试组之间的平均变化分别为0.306,0.177,0.081,0.088和0.107。
牙签作为亚硝酸盐检测用具
利用牙签作为亚硝酸盐检测用具的制备方式是以下试剂涂布,包含50mmol/L的对氨基苯磺酰胺(sulfanilamide)(≧99%,Sigma-Aldrich),330mmol/L的柠檬酸(≧99.5%,Sigma-Aldrich),和10mmol/L N-(1-萘基)乙二胺(N-(1-naphthyl)ethylenediamine)(≧98%,Sigma-Aldrich)。
图4显示牙签涂布亚硝酸盐检测试剂,并与不同浓度的亚硝酸盐溶液反应,由左到右的亚硝酸盐溶液浓度,分别为10,5,2.5,1.25,0.625和0.156mM。
搅拌棒的表面处理
藉由PDMS涂布搅拌棒以达成表面疏水性处理,之后将搅拌棒的一端浸于红墨水之中,请参照图5,结果发现经由PDMS涂布的搅拌棒其表面具有疏水性,因此红墨水仅由搅拌棒内部运送,并到达凹槽检测区,对照组的表面则充满红墨水,代表表面有红墨水通过。
利用搅拌棒检测亚硝酸盐
提供木质及竹制搅拌棒,将其以4μl PDMS进行表面处理,并将5μl的NO2 -检验试剂滴在凹槽内,其包含50mmol/L sulfanilamide,330mmol/L citric acid(≥99.5%,Sigma-Aldrich),10mmol/L N-(1-naphthyl)ethylenediamine。请参照图6,其显示木质及竹制搅拌棒涂布亚硝酸盐检测试剂,并与不同浓度的亚硝酸盐溶液反应,由左到右的亚硝酸盐溶液浓度,分别为0,156,625,2500μM。
综合上述,本发明的木质纤维检测用具的优势包括天然材质、价格便宜、加工方便,并可利用毛细作用使木质纤维基材成为疾病的预防检测平台及基材,并且可以让使用者不需花费额外的时间即可达成疾病预防检测。
以上所述的实施例仅系为说明本发明的技术思想及特点,其目的在使熟习此项技艺的人士能够了解本发明的内容并据以实施,当不能以的限定本发明的专利范围,即大凡依本发明所揭示的精神所作的均等变化或修饰,仍应涵盖在本发明的专利范围内。
Claims (11)
1.一种木质纤维检测用具,其特征在于包含:
一木质纤维基材;以及
至少一检测试剂,其涂布于该木质纤维基材的一木质纤维组织,其中当该木质纤维基材与一液态检体接触时,该液态检体藉由毛细作用吸附至该木质纤维基材,并与该检测试剂反应以达成检测该检体的作用。
2.如权利要求1所述的木质纤维检测用具,其特征在于该木质纤维基材为一搅拌棒、一牙签或一木筷。
3.如权利要求1所述的木质纤维检测用具,其特征在于该木质纤维组织为竹片。
4.如权利要求1所述的木质纤维检测用具,其特征在于该木质纤维基材包含一凹槽。
5.如权利要求4所述的木质纤维检测用具,其特征在于该检测试剂设置于该凹槽。
6.如权利要求4所述的木质纤维检测用具,其特征在于该凹槽的形状可包含长方体、正方体、圆柱、半球体、V型或上述形状组合。
7.如权利要求1所述的木质纤维检测用具,其特征在于该检测试剂包含一呈色剂或一荧光试剂。
8.如权利要求1所述的木质纤维检测用具,其特征在于该检测试剂包含一酵素试剂。
9.如权利要求1所述的木质纤维检测用具,其特征在于该液体检体系为一受试者的唾液、血液或尿液。
10.如权利要求1所述的木质纤维检测用具,其特征在于该木质纤维检测用具用以检测葡萄糖、亚硝酸根或酸碱度。
11.如权利要求1所述的木质纤维检测用具,其特征在于该木质纤维基材经疏水性表面处理。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW102113930A TW201441620A (zh) | 2013-04-19 | 2013-04-19 | 木質纖維檢測用具 |
TW102113930 | 2013-04-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN104111327A true CN104111327A (zh) | 2014-10-22 |
Family
ID=51708197
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310251514.XA Pending CN104111327A (zh) | 2013-04-19 | 2013-06-21 | 木质纤维检测用具 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20140315285A1 (zh) |
CN (1) | CN104111327A (zh) |
TW (1) | TW201441620A (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105717101A (zh) * | 2014-10-31 | 2016-06-29 | 郑兆珉 | 检测装置及其制造方法 |
CN106480160A (zh) * | 2015-09-01 | 2017-03-08 | 郑兆珉 | 微生物检测装置的制造方法、微生物检测方法、微生物检测试剂盒及装置 |
US9612203B2 (en) | 2013-06-25 | 2017-04-04 | National Tsing Hua University | Detection device and manufacturing method for the same |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2019174250A (ja) * | 2018-03-28 | 2019-10-10 | テルモ株式会社 | 試験片 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3732147A (en) * | 1970-09-17 | 1973-05-08 | Miles Lab | Colorimetric determination of dehydrogenases |
US3791929A (en) * | 1968-09-08 | 1974-02-12 | Searle & Co | Phosphatase assay using lyophilized aryl phosphate monesters |
US4320086A (en) * | 1981-01-05 | 1982-03-16 | Andre Reiss | Paper device for rapid detection of cocaine |
WO2001016599A1 (en) * | 1999-08-27 | 2001-03-08 | Viatech Imaging, Llc | Assay for detecting damage to the central nervous system |
WO2002004465A1 (en) * | 2000-07-06 | 2002-01-17 | Fal Diagnostics | Methods and kits for the detection of arginine compounds |
WO2003083436A2 (en) * | 2002-03-26 | 2003-10-09 | Dyax Corp. | Fibrinogen binding moieties |
US20130084226A1 (en) * | 2011-09-30 | 2013-04-04 | SAND COUNTY BIOTECHNOLOGY, Inc. | Sensing test block with rapid conductive reaction effect |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5185247A (en) * | 1989-03-10 | 1993-02-09 | Miles Inc. | Stabilization of oxidase enzyme-based test strips |
-
2013
- 2013-04-19 TW TW102113930A patent/TW201441620A/zh unknown
- 2013-06-21 CN CN201310251514.XA patent/CN104111327A/zh active Pending
- 2013-06-28 US US13/930,676 patent/US20140315285A1/en not_active Abandoned
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3791929A (en) * | 1968-09-08 | 1974-02-12 | Searle & Co | Phosphatase assay using lyophilized aryl phosphate monesters |
US3732147A (en) * | 1970-09-17 | 1973-05-08 | Miles Lab | Colorimetric determination of dehydrogenases |
US4320086A (en) * | 1981-01-05 | 1982-03-16 | Andre Reiss | Paper device for rapid detection of cocaine |
WO2001016599A1 (en) * | 1999-08-27 | 2001-03-08 | Viatech Imaging, Llc | Assay for detecting damage to the central nervous system |
WO2002004465A1 (en) * | 2000-07-06 | 2002-01-17 | Fal Diagnostics | Methods and kits for the detection of arginine compounds |
WO2003083436A2 (en) * | 2002-03-26 | 2003-10-09 | Dyax Corp. | Fibrinogen binding moieties |
US20130084226A1 (en) * | 2011-09-30 | 2013-04-04 | SAND COUNTY BIOTECHNOLOGY, Inc. | Sensing test block with rapid conductive reaction effect |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9612203B2 (en) | 2013-06-25 | 2017-04-04 | National Tsing Hua University | Detection device and manufacturing method for the same |
CN105717101A (zh) * | 2014-10-31 | 2016-06-29 | 郑兆珉 | 检测装置及其制造方法 |
TWI561819B (en) * | 2014-10-31 | 2016-12-11 | Univ Nat Tsing Hua | Three dimensional detection device and manufacturing method for the same |
CN106480160A (zh) * | 2015-09-01 | 2017-03-08 | 郑兆珉 | 微生物检测装置的制造方法、微生物检测方法、微生物检测试剂盒及装置 |
Also Published As
Publication number | Publication date |
---|---|
US20140315285A1 (en) | 2014-10-23 |
TW201441620A (zh) | 2014-11-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Ahmad et al. | A colorimetric pH sensor based on Clitoria sp and Brassica sp for monitoring of food spoilage using chromametry | |
Manchali et al. | Nutritional composition and health benefits of various botanical types of melon (Cucumis melo L.) | |
CN104111327A (zh) | 木质纤维检测用具 | |
CN105695363B (zh) | 一株降胆固醇、降亚硝酸盐的类植物乳杆菌及其筛选方法 | |
CN104611403B (zh) | 食品和餐饮具中大肠菌群快速检测纸片的制备与应用 | |
CN104777158A (zh) | 提供食品安全地图的方法、计算机程序产品和系统 | |
CN105969680A (zh) | 一株降胆固醇、降亚硝酸盐的戊糖乳杆菌及其筛选方法 | |
Herrera-Balandrano et al. | Feruloylated arabinoxylans from nixtamalized maize bran byproduct: A functional ingredient in frankfurter sausages | |
Chochevska et al. | Electrochemical determination of antioxidant capacity of traditional homemade fruit vinegars produced with double spontaneous fermentation | |
Kossyvaki et al. | Colorimetric indicators based on anthocyanin polymer composites: A review | |
Nogueira et al. | In vitro approach to evaluate potential harmful effects of beer on teeth | |
CN101529228B (zh) | 用于生物活性和/或生物可激活物质的检查和评估的设备和方法 | |
CN104251857A (zh) | 三维木质纤维检测用具 | |
CN102520182B (zh) | 一种可视血糖试纸及其制备方法 | |
Hamie et al. | Assessment of “sugranineteen” table grape maturation using destructive and auto-fluorescence methods | |
Ribeiro et al. | Halochromic Silk Fabric as a Reversible pH-Sensor Based on a Novel 2-Aminoimidazole Azo Dye | |
US9689854B2 (en) | Food safety detection device and manufacturing method for the same | |
Sun et al. | The inhibitory mechanism of eugenol on Lasiodiplodia theobromae and its induced disease resistance of passion fruit | |
CN104698203A (zh) | 具有线状传导功能的检测装置及其应用 | |
US9612203B2 (en) | Detection device and manufacturing method for the same | |
Artanti et al. | Antibacterial activity test of ethanol extract and SAP of Betung bamboo shoot (Dendrocalamus asper) against Klebsiella pneumoniae and Pseudomonas aeruginosa Bacteria | |
Firmansyah et al. | Identification of beef freshness using smart labels based on anthocyanin extract of purple cabbage (Brassica oleracea var. Capitata L.) | |
CN204556562U (zh) | 一种丝网印刷电极 | |
Lin et al. | DETECTION AND DISCRIMINATION OF ENTEROBACTER SAKAZAKII (CRONOBACTER SPP.) BY MID‐INFRARED SPECTROSCOPY AND MULTIVARIATE STATISTICAL ANALYSES | |
Szabo et al. | Application of Near Infrared Spectroscopy to Monitor the Quality Change of Sour Cherry Stored under Modified Atmosphere Conditions |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141022 |