CN104109693A - Microbial flocculant capable of treating strongly-alkaline waste water containing calcium ions and applications thereof - Google Patents
Microbial flocculant capable of treating strongly-alkaline waste water containing calcium ions and applications thereof Download PDFInfo
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- CN104109693A CN104109693A CN201410346676.6A CN201410346676A CN104109693A CN 104109693 A CN104109693 A CN 104109693A CN 201410346676 A CN201410346676 A CN 201410346676A CN 104109693 A CN104109693 A CN 104109693A
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Abstract
The invention relates to a microbial flocculant treating strongly-alkaline waste water containing calcium ions and applications thereof and belongs to the field of biological treatment of industrial waste water. The microbial flocculant is prepared by the following steps: inoculating Arthrobactersp. single clony into a culture medium, culturing and fermenting, and centrifuging a fermented culture liquid, so as to obtain an extracelluar supernatant, namely the microbial flocculant. The microbial flocculant can be directly used for treating the strongly-alkaline waste water containing calcium ions, the pH of the strongly-alkaline waste water containing calcium ions can be adjusted to be neutral by use of the microbial flocculant without adding a chemical flocculant, and pollutants in the waste water can be effectively removed. The microbial flocculant is simple to operate during use, the process of necessarily adding acid to reduce the pH of the to-be-treated waste water before use of the conventional microbial flocculant is omitted, the usage cost is lowered, the corrosion of acid to equipment can be avoided, and the microbial flocculant has greater development potential and good popularization value.
Description
Technical field
The invention belongs to trade effluent biological treatment field, be specifically related to a kind of microbial flocculant and application thereof of processing calcium ions strong basicity waste water.
Technical background
Alkaline waste water refers to contain certain bases, pH value higher than 9.0 waste water, can be divided into weakly alkaline waste water and strong basicity waste water according to pH difference, is mainly derived from the industry such as chemical industry, printing and dyeing, leather, oil refining, building.As discharged without processing, by corrosion pipeline, channel and hydraulic structure; After entering water body, will change its pH, destroy water body natural, ecological, aquaculture will be caused to serious threat; Infiltrate soil and easily cause salting of soil, affect agriculture production.The treatment process of alkaline waste water mainly contains acid-base neutralisation method, flocculence, chemical precipitation method and crystallization process at present.Wherein flocculence receives much concern in recent years because having good environmental economical profit.Flocculence is mainly realized by chemical floc and biological flocculant.Compared with chemical floc, biological flocculant has the advantages such as readily biodegradable, environmental hazard are little, non-secondary pollution.And microbial flocculant forms the core member into biological flocculant because its preparation is simple, production cost is low, with short production cycle etc.Microbial flocculant is mainly made up of biomacromolecule materials such as the glycoprotein of microorganism cells or microorganisms producing, polysaccharide, albumen.
Organic content (COD) contained in the cyclisation waste water that the process of employing chlorohydrination production propylene oxide and epoxy chloropropane is discharged, at 1000～2000mg/L, needs to process rear discharge.And because this cyclisation waste water has high calcium chloride content and high pH value, bring certain difficulty to processing.For reducing intractability, the method that manufacturer all adopts thin up calcium chloride concentration, acid adding to reduce pH value is processed this waste water, so that increase treatment scale and working cost.In this type of calcium ions strong basicity waste water process of processing, most of microbial flocculant can lose flocculation activity, causes its flocculating effect not good.Therefore in the urgent need to developing a kind of microbial flocculant for the wastewater treatment of calcium ions strong basicity.
Summary of the invention
For the deficiency of existing microbial flocculant, the invention provides a kind of microbial flocculant and application thereof of processing calcium ions strong basicity waste water, microbial flocculant of the present invention uses non-secondary pollution, production technique is simple, fermentation period is short, with low cost, and industrial application potentiality are large.
In order to realize object of the present invention, contriver utilizes Arthrobacter (Arthrobacter sp.) to prepare a kind of microbial flocculant for calcium ions strong basicity waste water, and uses it for wastewater treatment, has obtained desirable result of use.
A kind of microbial flocculant of processing calcium ions strong basicity waste water prepared by the present invention, made by following methods:
The single colony inoculation of a picking Arthrobacter (Arthrobacter sp.), in LB seed culture medium, is cultivated, and obtains nutrient solution;
B is inoculated in above-mentioned nutrient solution in fermention medium, and fermentation culture obtains fermentation culture;
C is by centrifugal above-mentioned fermentation culture, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant.
Described, LB seed culture based component: peptone 5-12g/L, yeast powder 2-8g/L, sodium-chlor 5-12g/L; Fermentation culture based component: glucose 5-20g/L, yeast powder 1-5g/L, potassium primary phosphate 2-8g/L.
Described, step a incubation time 12-16h, culture temperature 25-37 DEG C, shaking speed 150-200r/min; Step b inoculum size 2-10%, incubation time 65-80h, culture temperature 25-32 DEG C, shaking speed 160-220r/min; Step c centrifugation time 5-15min, microbial flocculant is containing polysaccharide 50-70%, protein 30-50% and nucleic acid 0.1-1.0%.
Described, Arthrobacter is existing conventional bacterial classification, can buy and obtain from American Type Culture Collecti (ATCC).
Described Arthrobacter is ATCC31012.Arthrobacter is one of bacterium the most common in all soil bacterias, has obvious growth cycle, and obligate is aerobic, chemoheterotrophy, respiratory metabolism, effectively various organism and metal ion in degradation water.
Preferably, LB seed culture based component: peptone 10g/L, yeast powder 5g/L, sodium-chlor 10g/L; Fermentation culture based component: glucose 10g/L, yeast powder 2.5g/L, potassium primary phosphate 5g/L.
Preferably, step a incubation time 14h, 30 DEG C of culture temperature, shaking speed 180r/min; Step b inoculum size 5%, incubation time 72h, 30 DEG C of culture temperature, shaking speed 200r/min; Step c centrifugation time 10min, microbial flocculant is containing polysaccharide 63%, protein 36% and nucleic acid 0.5%.
Microbial flocculant is processed calcium ions strong basicity waste water:
The microbial flocculant that present inventor is made adds in the calcium ions strong basicity waste water of pending pH 10.5-14, add-on is pressed the volume ratio 1:12-25 of microbial flocculant and waste water, stir 20-50s with 100-300r/min speed, staticly settle 5-20min, flocculated suspension material.
Preferably, wastewater pH 12-14, the volume ratio 1:18 of microbial flocculant and waste water, stirring velocity 200r/min, churning time 30s, staticly settles time 10min.
Described, waste water is the waste water that solvay soda process alkaline, chlorohydrination are produced propylene oxide or epoxy chloropropane generation.
Microbial flocculant of the present invention can be directly used in calcium ions strong basicity Industrial Wastewater Treatment, without adding chemical floc, equipment is simple, the pH of calcium ions strong basicity waste water can be down to neutrality, and effectively remove the pollutent in waste water by microbial flocculant.Simple to operate in use procedure, must acid adding before having omitted conventional microbial flocculant and using reduce the process of pending wastewater pH, reduce use cost, avoid the sour corrosion to equipment.
Embodiment
In order to make object of the present invention, technical scheme and advantage more clear, below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
The preparation of microbial flocculant:
A is inoculated in Arthrobacter ATCC31012 in LB seed culture medium and cultivates 12h, 37 DEG C of culture temperature, and shaking speed 150r/min, obtains nutrient solution;
B is inoculated in above-mentioned nutrient solution in fermention medium and cultivates 65h by inoculum size 10%, 32 DEG C of culture temperature, and shaking speed 160r/min, obtains fermentation culture;
C is by centrifugal above-mentioned fermentation culture 15min, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant.
LB seed culture based component is: peptone 5g/L, yeast powder 2g/L, sodium-chlor 18g/L.
Fermentation culture based component is: glucose 20g/L, yeast powder 1g/L, potassium primary phosphate 2g/L.
Microbial flocculant is containing polysaccharide 50%, protein 50% and nucleic acid 0.1%.
Microbial flocculant is processed calcium ions strong basicity waste water:
The microbial flocculant that the present embodiment is made adds in pending calcium ions strong basicity waste water, and add-on is pressed the volume ratio 1:12 of microbial flocculant and waste water, stirs 50s with 100r/min speed, staticly settles 20min, flocculated suspension material.
Embodiment 2
The preparation of microbial flocculant:
A is inoculated in Arthrobacter ATCC31012 in LB seed culture medium and cultivates 14h, 30 DEG C of culture temperature, and shaking speed 180r/min, obtains nutrient solution;
B is inoculated in above-mentioned nutrient solution in fermention medium and cultivates 72h by inoculum size 5%, 30 DEG C of culture temperature, and shaking speed 200r/min, obtains fermentation culture;
C is by centrifugal above-mentioned fermentation culture 10min, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant.
LB seed culture based component is: peptone 10g/L, yeast powder 5g/L, sodium-chlor 10g/L.
Fermentation culture based component is: glucose 10g/L, yeast powder 2.5g/L, potassium primary phosphate 5g/L.
Microbial flocculant is containing polysaccharide 63%, protein 36% and 0.5% nucleic acid.
Microbial flocculant is processed calcium ions strong basicity waste water:
The microbial flocculant that the present embodiment is made adds in pending calcium ions strong basicity waste water, and add-on is pressed the volume ratio 1:18 of microbial flocculant and waste water, stirs 30s with 200r/min speed, staticly settles 10min, flocculated suspension material.
Embodiment 3
The preparation of microbial flocculant:
A is inoculated in Arthrobacter ATCC31012 in LB seed culture medium and cultivates 16h, 25 DEG C of culture temperature, and shaking speed 200r/min, obtains nutrient solution;
B is inoculated in above-mentioned nutrient solution in fermention medium and cultivates 80h by inoculum size 2%, 25 DEG C of culture temperature, and shaking speed 220r/min, obtains fermentation culture;
C is by centrifugal above-mentioned fermentation culture 5min, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant.
LB seed culture based component is: peptone 15g/L, yeast powder 10g/L, sodium-chlor 6g/L.
Fermentation culture based component is: glucose 5g/L, yeast powder 5g/L, potassium primary phosphate 12g/L.
Microbial flocculant is containing polysaccharide 70%, protein 30% and 1.0% nucleic acid.
Microbial flocculant is processed calcium ions strong basicity waste water:
The microbial flocculant that the present embodiment is made adds in pending calcium ions strong basicity waste water, and add-on is pressed the volume ratio 1:25 of microbial flocculant and waste water, stirs 20s with 300r/min speed, staticly settles 5min, flocculated suspension material.
Further illustrate beneficial effect of the present invention below by effect embodiment.
Effect embodiment 1
The simulated experiment of flocculating effect is detected in laboratory, investigates the effect of embodiment and conventional flocculation agent.
The mensuration of flocculating rate: to the 1%CaCl that adds 0.5g kaolin, 3mL in 100mL colorimetric cylinder
2solution and 2mL microbial flocculant, then adding distil water is to 100mL (A), identical but replace microbial flocculant (B) in contrast with the fermention medium of 2mL equal conditions with other conditions, cover grinding port plug, colorimetric cylinder is overturn and the 2min that vibrates up and down naturally, must make kaolin and CaCl
2solution and flocculation agent dissolve completely and fully mix static 5min;
Get the treatment solution at 50mL place in colorimetric cylinder, with its absorbancy OD at 550nm wavelength place of 722 type spectrophotometric determinations
550nm, using distilled water as blank, the calculation formula of flocculating rate (Flocculation Rate, FR, %) is as follows:
FR(%)=(B-A)/B×100；
Comparative example 1: conventional commercially available polymerize aluminum chloride.
Comparative example 2: uncured compound bio fermented liquid.
Comparative example 3: conventional commercially available anionic polyacrylamide.
Table 1 embodiment and the comparison of dissimilar flocculation agent flocculating property
| Classification | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| FR(%) | 91.45±1.4 | 94.56±1.5 | 92.13±2.3 | 77.01±1.6 | 88.15±1.8 | 86.28±2.1 |
Effect embodiment 2
The effect of certain chemical plant epoxy propane saponified wastewater, from epoxy propane saponified wastewater, take out waste water, be divided into six 100mL, add respectively embodiment 1-3 makes before 0.2g microbial flocculant, 0.2g polymerize aluminum chloride (comparative example 1), the uncured compound bio fermented liquid of 0.2g (comparative example 2) and 0.2 g polyacrylamide (comparative example 3), shake up, static 30min, gets supernatant liquor and measures OD
550nmand pH.
Table 2 epoxy propane saponified wastewater treatment effect
| ? | PH value | COD（mg/L） | Flocculating rate (%) |
| Before flocculation | 12.5 | 1058 | — |
| Embodiment 1 | 6.5±0.2 | 135±2 | 94.72±1.8 |
| Embodiment 2 | 6.8±0.3 | 118±3 | 95.17±2.4 |
| Embodiment 3 | 7.0±0.2 | 126±2 | 94.28±1.4 |
| Comparative example 1 | 11.1±0.4 | 157±1 | 83.45±1.9 |
| Comparative example 2 | 10.8±0.2 | 149±2 | 82.19±1.6 |
| Comparative example 3 | 11.0±0.1 | 152±3 | 79.14±2.1 |
In sum, than prior art, beneficial effect of the present invention is as follows: (1) the invention provides a kind of microbial flocculant of processing calcium ions strong basicity waste water, avoid prior art acid adding to reduce the process of pending wastewater pH, reduce use cost, avoided the corrosion of acid to equipment; (2) microorganism used therefor flocculation agent flocculation agent activity is high, and fermentation time is short, and technique is simple, greatly reduces production costs; (3) microbial flocculant applicable pH value scope of the present invention, 10.5～14, has overcome general flocculation agent at this pH value scope flocculating effect extreme difference, can not reduce the shortcoming of pending wastewater pH, has larger development potentiality and good promotional value.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (8)
1. process a microbial flocculant for calcium ions strong basicity waste water, made by following methods:
The single colony inoculation of a picking Arthrobacter (Arthrobacter sp.), in LB seed culture medium, is cultivated, and obtains nutrient solution;
B is inoculated in above-mentioned nutrient solution in fermention medium, and fermentation culture obtains fermentation culture;
C is by centrifugal above-mentioned fermentation culture, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant.
2. microbial flocculant according to claim 1, is characterized in that LB seed culture based component: peptone 5-12g/L, yeast powder 2-8g/L, sodium-chlor 5-12g/L; Fermentation culture based component: glucose 5-20g/L, yeast powder 1-5g/L, potassium primary phosphate 2-8g/L.
3. microbial flocculant according to claim 1, is characterized in that step a incubation time 12-16h, culture temperature 25-37 DEG C, shaking speed 150-200r/min; Step b inoculum size 2-10%, incubation time 65-80h, culture temperature 25-32 DEG C, shaking speed 160-220r/min; Step c centrifugation time 5-15min, microbial flocculant is containing polysaccharide 50-70%, protein 30-50% and nucleic acid 0.1-1.0%.
4. microbial flocculant according to claim 2, is characterized in that LB seed culture based component: peptone 10g/L, yeast powder 5g/L, sodium-chlor 10g/L; Fermentation culture based component: glucose 10g/L, yeast powder 2.5g/L, potassium primary phosphate 5g/L.
5. microbial flocculant according to claim 3, is characterized in that step a incubation time 14h, 30 DEG C of culture temperature, shaking speed 180r/min; Step b inoculum size 5%, incubation time 72h, 30 DEG C of culture temperature, shaking speed 200r/min; Step c centrifugation time 10min, microbial flocculant is containing polysaccharide 63%, protein 36% and nucleic acid 0.5%.
6. the application of the microbial flocculant described in any one in a claim 1 to 5, it is characterized in that microbial flocculant to add in the calcium ions strong basicity waste water of pending pH 10.5-14, add-on is pressed the volume ratio 1:12-25 of microbial flocculant and waste water, stir 20-50s with 100-300r/min speed, staticly settle 5-20min, flocculated suspension material.
7. the application of microbial flocculant according to claim 6, is characterized in that wastewater pH 12-14, the volume ratio 1:18 of microbial flocculant and waste water, and stirring velocity 200r/min, churning time 30s, staticly settles time 10min.
8. the application of microbial flocculant according to claim 6, is characterized in that described waste water is the waste water that solvay soda process alkaline, chlorohydrination are produced propylene oxide or epoxy chloropropane generation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104528952A (en) * | 2014-07-21 | 2015-04-22 | 济南大学 | Compound microbial flocculant processing strong-alkali waste water without calcium ions and application thereof |
| CN111333266A (en) * | 2020-03-10 | 2020-06-26 | 青海清循水处理技术推广中心 | Method for preparing new regenerated brine by recycling regenerated wastewater |
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| CN103173497A (en) * | 2013-03-11 | 2013-06-26 | 宁波飞日水产实业有限公司 | Microbial flocculating agent preparation method |
| CN103214101A (en) * | 2012-01-19 | 2013-07-24 | 中国中化股份有限公司 | Microbial flocculating agent, and preparation and use thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103214101A (en) * | 2012-01-19 | 2013-07-24 | 中国中化股份有限公司 | Microbial flocculating agent, and preparation and use thereof |
| CN103173497A (en) * | 2013-03-11 | 2013-06-26 | 宁波飞日水产实业有限公司 | Microbial flocculating agent preparation method |
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| Title |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104528952A (en) * | 2014-07-21 | 2015-04-22 | 济南大学 | Compound microbial flocculant processing strong-alkali waste water without calcium ions and application thereof |
| CN111333266A (en) * | 2020-03-10 | 2020-06-26 | 青海清循水处理技术推广中心 | Method for preparing new regenerated brine by recycling regenerated wastewater |
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