CN104528952A - Compound microbial flocculant processing strong-alkali waste water without calcium ions and application thereof - Google Patents
Compound microbial flocculant processing strong-alkali waste water without calcium ions and application thereof Download PDFInfo
- Publication number
- CN104528952A CN104528952A CN201510003122.0A CN201510003122A CN104528952A CN 104528952 A CN104528952 A CN 104528952A CN 201510003122 A CN201510003122 A CN 201510003122A CN 104528952 A CN104528952 A CN 104528952A
- Authority
- CN
- China
- Prior art keywords
- microbial flocculant
- waste water
- effective microorganisms
- chloride solution
- calcium chloride
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/16—Nature of the water, waste water, sewage or sludge to be treated from metallurgical processes, i.e. from the production, refining or treatment of metals, e.g. galvanic wastes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/22—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof
- C02F2103/24—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof from tanneries
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/30—Nature of the water, waste water, sewage or sludge to be treated from the textile industry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention relates to a compound microbial flocculant processing strong-alkali waste water without calcium ions and an application of the compound microbial flocculant and belongs to the field of industrial waste water processing. A microbial flocculant and a calcium chloride solution are mixed, and the compound microbial flocculant is prepared. The compound microbial flocculant is obtained in the mode that under a certain conditions, Arthrobacter sp is inoculated to a culture medium to be cultured and fermented, the fermented culture solution is subjected to centrifugation, and obtained extracellular supernatant is the compound microbial flocculant. The compound microbial flocculant is used in cooperation with the calcium chloride solution, complementation is achieved, the problem that a singular microbial flocculant has a poor effect on the strong-alkali waste water without calcium ions is avoided, the flocculation efficiency is remarkably improved, and the pH of waste water is obviously lowered. The compound microbial flocculant is easy to use, high in flocculation efficiency, low in dosage, and low in production cost.
Description
Technical field
The invention belongs to trade effluent biological treatment field, be specifically related to a kind of effective microorganisms and the application thereof that process not calcium ions strong basicity waste water.
Technical background
Alkaline waste water refers to contain certain bases, pH value higher than the waste water of 9.0, can be divided into weakly alkaline waste water and strong basicity waste water, be mainly derived from the industry such as chemical industry, printing and dyeing, leather, oil refining, building according to pH difference.As without process discharge, by corrosion pipeline, channel and hydraulic structure; Its pH will be changed after entering water body, and destroy water body natural, ecological, serious threat is caused to aquaculture; Infiltrate soil and then easily cause salting of soil, affect agriculture production.The treatment process of current alkaline waste water mainly contains acid-base neutralisation method, flocculence, chemical precipitation method and crystallization process.Wherein flocculence receives much concern in recent years because having good environmental economical profit.Flocculence realizes primarily of chemical floc and biological flocculant.Compared with chemical floc, biological flocculant has the advantages such as readily biodegradable, environmental hazard are little, non-secondary pollution.Especially microbial flocculant becomes the core member of biological flocculant because having the advantages such as preparation is simple, production cost is low, with short production cycle.But the restriction of the condition such as molecular structure, spatial form, molecular weight, group due to himself, makes its flocculation ability have certain limitation.Traditional inorganic flocculating agent can make up the shortcoming of microbial flocculant, but when being used alone, dosage is large, and working cost is high, and the precipitating sludge amount produced is large.If both can be with the use of, realize complementary, so not only can improve flocculation efficiency, reduce flocculant dosage, cost, the kind of process waste water can also be widened.
Known 2012 are seen, " polymeric aluminum-modification Compound biological flocculant inorganic organic composite coagulator and preparation method thereof " (application number is 201210123810.7) of Shandong University's application for a patent for invention, by effective microorganisms and acrylamide and the dimethyl diallyl ammonium chloride modified composite biological flocculant that graft copolymerization is obtained under Potassium Persulphate and S-WAT initiator exist and polymeric aluminum (AlCl
36H
2o raw material is dissolved in distilled water, adds Na
2cO
3regulate basicity, normal-temperature reaction obtains) be mixed in proportion and react obtained, make full use of the characteristic of aluminum chloride, acrylamide and biological flocculant, but residual aluminum ion and acrylamide can be there is in sewage, form secondary pollution.And have efficient, cheap, have no report for the effective microorganisms of the not strong basicity waste water of calcium ions.
Priority matters:
The applying date: on 07 21st, 2014.Application number: 2014103466056.Applicant: University Of Ji'nan.Invention and created name: a kind of process is the effective microorganisms of calcium ions strong basicity waste water and application thereof not.
Summary of the invention
For the deficiency of existing microbial flocculant, the invention provides a kind of effective microorganisms and the application thereof that process not calcium ions strong basicity waste water, effective microorganisms of the present invention uses flocculation efficiency high, and dosage is low, with strong points.
In order to realize object of the present invention, to have utilized Arthrobacter (Arthrobacter sp.) to prepare a kind of for contriver for the effective microorganisms of not calcium ions strong basicity waste water, and use it for the strong basicity wastewater treatment of not calcium ions, achieve extraordinary result of use.
A kind of microbial flocculant processing not calcium ions strong basicity waste water prepared by the present invention, be made up of following methods:
A picking Arthrobacter (Arthrobacter sp.) single colony inoculation, in LB seed culture medium, is cultivated, is obtained nutrient solution;
Above-mentioned nutrient solution is inoculated in fermention medium by b, and fermentation culture obtains fermentation culture;
C is by centrifugal for above-mentioned fermentation culture, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant;
D prepares certain density calcium chloride solution, then adds mentioned microorganism flocculation agent, stirs, and both obtains effective microorganisms.
Described, LB seed culture based component: peptone 5-15g/L, yeast powder 2-8g/L, sodium-chlor 5-12g/L; Fermentation medium components: glucose 5-15g/L, yeast powder 1-5g/L, potassium primary phosphate 1-8g/L.
Described, step a incubation time 10-20h, culture temperature 25-37 DEG C, shaking speed 120-200r/min; Step b inoculum size 3-10%, incubation time 60-80h, culture temperature 25-32 DEG C, shaking speed 160-250r/min; Step c centrifugation time 5-15min, microbial flocculant is containing polysaccharide 50-70%, protein 30-50% and nucleic acid 0.1-1.0%; The concentration 0.4-1.6% of steps d calcium chloride solution, in effective microorganisms, the volume ratio of calcium chloride solution and microbial flocculant is 1:0.5-2.
Described, Arthrobacter is existing conventional bacterial classification, can buy obtain from American Type Culture Collecti (ATCC).
Described Arthrobacter is ATCC31012.Arthrobacter is one of bacterium the most common in all soil bacterias, has obvious growth cycle, obligate aerobic, chemoheterotrophy, respiratory metabolism, can effectively various organism and adsorbing metal ions in degradation water.
Preferably, LB seed culture based component: peptone 10g/L, yeast powder 5g/L, sodium-chlor 10g/L; Fermentation medium components: glucose 10g/L, yeast powder 2.5g/L, potassium primary phosphate 4g/L.
Preferably, step a incubation time 15h, culture temperature 28 DEG C, shaking speed 150r/min; Step b inoculum size 5%, fermention medium incubation time 72h, culture temperature 30 DEG C, shaking speed 200r/min; Step c centrifugation time 10min, microbial flocculant is containing polysaccharide 65%, protein 35% and nucleic acid 0.5%; The concentration 0.8% of steps d calcium chloride solution, in effective microorganisms, the volume ratio of calcium chloride solution and microbial flocculant is 1:1.
Effective microorganisms process is calcium ions strong basicity waste water not:
Effective microorganisms the present invention obtained adds in the not calcium ions strong basicity waste water of pending pH 10.5-14, add-on compares 1:6-13 by effective microorganisms and wastewater volume, stir 20-50s with 100-300r/min speed, staticly settle 5-20min, flocculated suspension material.
Preferably, wastewater pH 12-14, the volume ratio 1:9 of effective microorganisms and waste water, stirring velocity 200r/min, churning time 30s, staticly settle time 10min.
Described, waste water is the waste water of leather, printing and dyeing, plating generation.
Effective microorganisms of the present invention can be directly used in not calcium ions strong basicity Industrial Wastewater Treatment, solves the single microorganism flocculation agent problem that result of use is general in not calcium ions strong basicity waste water.Effective microorganisms of the present invention uses simple, and significantly reduce the pH of waste water, flocculation efficiency is high, and flocculant dosage is low, and production cost is low.
Embodiment
In order to make object of the present invention, technical scheme and advantage more clear, below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
The preparation of effective microorganisms:
Arthrobacter ATCC31012 is inoculated in LB seed culture medium and cultivates 14h by a, culture temperature 30 DEG C, and shaking speed 180r/min, obtains nutrient solution;
Above-mentioned nutrient solution is inoculated in fermention medium by inoculum size 10% and cultivates 72h, culture temperature 30 DEG C by b, and shaking speed 200r/min, obtains fermentation culture;
C is by centrifugal for above-mentioned fermentation culture 10min, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant;
The calcium chloride solution of d preparation 0.4%, is then that 1:2 adds mentioned microorganism flocculation agent by the volume ratio of calcium chloride solution and microbial flocculant, stirs, both effective microorganisms.
LB seed culture based component is: peptone 15g/L, yeast powder 2g/L, sodium-chlor 12g/L.
Fermentation medium components is: glucose 5g/L, yeast powder 5g/L, potassium primary phosphate 1g/L.
Microbial flocculant is containing polysaccharide 65%, protein 35% and 0.5% nucleic acid.
Effective microorganisms process is calcium ions strong basicity waste water not:
The effective microorganisms obtained by the present embodiment adds in pending not calcium ions strong basicity waste water, and add-on presses effective microorganisms and wastewater volume than 1:6, stirs 50s, staticly settle 20min, flocculated suspension material with 100r/min speed.
Embodiment 2
The preparation of effective microorganisms:
Arthrobacter ATCC31012 is inoculated in LB seed culture medium and cultivates 14h by a, culture temperature 30 DEG C, and shaking speed 180r/min, obtains nutrient solution;
Above-mentioned nutrient solution is inoculated in fermention medium by inoculum size 5% and cultivates 72h, culture temperature 30 DEG C by b, and shaking speed 200r/min, obtains fermentation culture;
C is by centrifugal for above-mentioned fermentation culture 10min, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant;
The calcium chloride solution of d preparation 0.8%, is then that 1:1 adds mentioned microorganism flocculation agent by the volume ratio of calcium chloride solution and microbial flocculant, stirs, both effective microorganisms.
LB seed culture based component is: peptone 12g/L, yeast powder 5g/L, sodium-chlor 10g/L.
Fermentation medium components is: glucose 10g/L, yeast powder 2.5g/L, potassium primary phosphate 4g/L.
Microbial flocculant is containing polysaccharide 65%, protein 35% and 0.5% nucleic acid.
Effective microorganisms process is calcium ions strong basicity waste water not:
The effective microorganisms obtained by the present embodiment adds in pending not calcium ions strong basicity waste water, and add-on presses effective microorganisms and wastewater volume than 1:9, stirs 30s, staticly settle 10min, flocculated suspension material with 200r/min speed.
Embodiment 3
The preparation of effective microorganisms:
Arthrobacter ATCC31012 is inoculated in LB seed culture medium and cultivates 16h by a, culture temperature 25 DEG C, and shaking speed 200r/min, obtains nutrient solution;
Above-mentioned nutrient solution is inoculated in fermention medium by inoculum size 3% and cultivates 80h, culture temperature 25 DEG C by b, and shaking speed 220r/min, obtains fermentation culture;
C is by centrifugal for above-mentioned fermentation culture 5min, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant;
The calcium chloride solution of d preparation 1.6%, is then that 1:0.5 adds mentioned microorganism flocculation agent by the volume ratio of calcium chloride solution and microbial flocculant, stirs, both effective microorganisms.
LB seed culture based component is: peptone 5g/L, yeast powder 8g/L, sodium-chlor 5g/L.
Fermentation medium components is: glucose 15g/L, yeast powder 1g/L, potassium primary phosphate 8g/L.
Microbial flocculant is containing polysaccharide 70%, protein 30% and 1.0% nucleic acid.
Effective microorganisms process is calcium ions strong basicity waste water not:
The effective microorganisms obtained by the present embodiment adds in pending not calcium ions strong basicity waste water, and add-on presses effective microorganisms and wastewater volume than 1:13, stirs 20s, staticly settle 5min, flocculated suspension material with 300r/min speed.
Effect example 1
The simulated experiment of test in laboratory flocculating effect, investigates the effect of embodiment and conventional flocculation agent.
The mensuration of flocculating rate: the 1%CaCl adding 0.5g kaolin, 3mL in 100mL colorimetric cylinder
2solution and 2mL effective microorganisms, then adding distil water is to 100mL (A), identical but replace flocculation agent in contrast (B) with the fermention medium of 2mL equal conditions with other conditions, cover grinding port plug, colorimetric cylinder is overturn and the 2min that vibrates up and down naturally, kaolin and CaCl must be made
2solution and flocculation agent dissolve completely and fully mix, static 5min;
Get the treatment solution at 50mL place in colorimetric cylinder, with its absorbancy OD at 550nm wavelength place of 722 type spectrophotometric determinations
550nm, using distilled water as blank, the calculation formula of flocculating rate (Flocculation Rate, FR, %) is as follows:
FR(%)=(B-A)/B×100；
Comparative example 1: the microbial flocculant that embodiment 2 is obtained.
Comparative example 2: uncured compound bio fermented liquid.
Comparative example 3: conventional commercial anionic polyacrylamide.
Result is as shown in table 1.
Table 1 embodiment compares with dissimilar flocculation agent flocculating property
| Classification | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| FR(%) | 90.82±1.2 | 93.96±1.7 | 93.03±2.1 | 84.75±1.4 | 89.13±1.7 | 85.87±2.2 |
Effect example 2
The effect of certain dye house effluents, waste water is taken out from dyeing waste water, be divided into six 100mL, add microbial flocculant (comparative example 1), the uncured compound bio fermented liquid of 0.2g (comparative example 2) and 0.2g polyacrylamide (comparative example 3) that effective microorganisms that 0.2g preceding embodiment 1-3 obtains, 0.2g embodiment 2 are obtained respectively, shake up, static 30min, gets supernatant liquor and measures OD
550nmand pH, result is as shown in table 2.
Table 2 disposal effect of dyeing wastewater
| PH value | COD（mg/L） | Flocculating rate (%) | |
| Before flocculation | 11.9 | 859 | — |
| Embodiment 1 | 6.7±0.3 | 132±2 | 93.92±2.4 |
| Embodiment 2 | 6.6±0.1 | 117±3 | 95.01±1.8 |
| Embodiment 3 | 6.9±0.2 | 128±2 | 94.54±1.4 |
| Comparative example 1 | 9.9±0.4 | 152±2 | 84.95±1.6 |
| Comparative example 2 | 10.5±0.1 | 145±1 | 82.08±1.8 |
| Comparative example 3 | 10.8±0.2 | 149±1 | 78.74±2.0 |
In sum, compared to prior art, beneficial effect of the present invention is as follows: (1) the invention provides a kind of effective microorganisms processing not calcium ions strong basicity waste water, prior art acid adding is avoided to reduce the process of pending wastewater pH, reduce use cost, avoid the corrosion of acid to equipment; (2) effective microorganisms of the present invention is suitable for pH value range 10.5 ~ 14, overcomes general flocculation agent at this pH value range flocculating effect extreme difference, can not reduce the shortcoming of pending wastewater pH; (3) single microorganism flocculation agent result of use in not calcium ions strong basicity waste water is general, by microorganism and calcium chloride solution with the use of, realize complementary, significantly improve flocculation efficiency, significantly can reduce the pH of waste water simultaneously.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. process an effective microorganisms for not calcium ions strong basicity waste water, it is characterized in that, be made up of following methods:
A picking Arthrobacter (Arthrobacter sp.) single colony inoculation, in LB seed culture medium, is cultivated, is obtained nutrient solution;
Above-mentioned nutrient solution is inoculated in fermention medium by b, and fermentation culture obtains fermentation culture;
C is by centrifugal for above-mentioned fermentation culture, and the outer supernatant liquor of gained born of the same parents, is microbial flocculant;
D prepares certain density calcium chloride solution, then adds mentioned microorganism flocculation agent, stirs, and both obtains effective microorganisms.
2. microbial flocculant according to claim 1, is characterized in that, LB seed culture based component: peptone 5-15g/L, yeast powder 2-8g/L, sodium-chlor 5-12g/L; Fermentation medium components: glucose 5-15g/L, yeast powder 1-5g/L, potassium primary phosphate 1-8g/L.
3. microbial flocculant according to claim 1, is characterized in that, step a incubation time 10-20h, culture temperature 25-37 DEG C, shaking speed 120-200r/min; Step b inoculum size 3-10%, incubation time 60-80h, culture temperature 25-32 DEG C, shaking speed 160-250r/min; Step c centrifugation time 5-15min, microbial flocculant is containing polysaccharide 50-70%, protein 30-50% and nucleic acid 0.1-1.0%; The concentration 0.4-1.6% of steps d calcium chloride solution, in effective microorganisms, the volume ratio of calcium chloride solution and microbial flocculant is 1:0.5-2.
4. effective microorganisms according to claim 2, is characterized in that, LB seed culture based component: peptone 12g/L, yeast powder 5g/L, sodium-chlor 10g/L; Fermentation medium components: glucose 10g/L, yeast powder 2.5g/L, potassium primary phosphate 4g/L.
5. effective microorganisms according to claim 3, is characterized in that, step a incubation time 15h, culture temperature 28 DEG C, shaking speed 150r/min; Step b inoculum size 5%, fermention medium incubation time 72h, culture temperature 30 DEG C, shaking speed 200r/min; Step c centrifugation time 10min, microbial flocculant is containing polysaccharide 65%, protein 35% and nucleic acid 0.5%; The concentration 0.8% of steps d calcium chloride solution, in effective microorganisms, the volume ratio of calcium chloride solution and microbial flocculant is 1:1.
6. the application of the effective microorganisms according to any one of a claim 1 to 5, it is characterized in that, effective microorganisms is added in the not calcium ions strong basicity waste water of pending pH 10.5-14, add-on compares 1:6-13 by effective microorganisms and wastewater volume, 20-50s is stirred with 100-300r/min speed, staticly settle 5-20min, flocculated suspension material.
7. the application of effective microorganisms according to claim 6, is characterized in that, described wastewater pH 12-14, and the volume ratio 1:9 of effective microorganisms and waste water, stirring velocity 200r/min, churning time 30s staticly settle time 10min.
8. the application of effective microorganisms according to claim 6, is characterized in that, described waste water is the waste water of leather, printing and dyeing, electroplating industry generation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510003122.0A CN104528952B (en) | 2014-07-21 | 2015-01-06 | A kind of effective microorganisms processing not calcium ions strong basicity waste water and application thereof |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410346605 | 2014-07-21 | ||
| CN2014103466056 | 2014-07-21 | ||
| CN201510003122.0A CN104528952B (en) | 2014-07-21 | 2015-01-06 | A kind of effective microorganisms processing not calcium ions strong basicity waste water and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104528952A true CN104528952A (en) | 2015-04-22 |
| CN104528952B CN104528952B (en) | 2016-07-20 |
Family
ID=52844646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510003122.0A Expired - Fee Related CN104528952B (en) | 2014-07-21 | 2015-01-06 | A kind of effective microorganisms processing not calcium ions strong basicity waste water and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104528952B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4363733A (en) * | 1978-12-28 | 1982-12-14 | Rhone-Poulenc Industries | Process for water treatment by flocculation |
| US4855051A (en) * | 1987-05-11 | 1989-08-08 | Polysar Limited | Microbial treatment of wastewater to remove tertiary butyl alcohol |
| CN1616358A (en) * | 2003-11-13 | 2005-05-18 | 栾兴社 | Method for preparing biological flocculant from arthrobacter |
| CN1686850A (en) * | 2005-04-08 | 2005-10-26 | 山东金佰生物技术有限公司 | Downstream extraction technique for production flocculant of microbe |
| CN1911825A (en) * | 2006-08-04 | 2007-02-14 | 哈尔滨工业大学 | Compound biological flocculant |
| CN101555054A (en) * | 2009-05-19 | 2009-10-14 | 山东齐东方投资有限公司 | Method for extracting microbial polysaccharide flocculant by membrane concentration and pneumatic drying method |
| CN101560536A (en) * | 2009-05-19 | 2009-10-21 | 山东齐东方投资有限公司 | Fermentation method of microbial polysaccharide flocculant |
| CN104109693A (en) * | 2014-07-21 | 2014-10-22 | 济南大学 | Microbial flocculant capable of treating strongly-alkaline waste water containing calcium ions and applications thereof |
-
2015
- 2015-01-06 CN CN201510003122.0A patent/CN104528952B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4363733A (en) * | 1978-12-28 | 1982-12-14 | Rhone-Poulenc Industries | Process for water treatment by flocculation |
| US4855051A (en) * | 1987-05-11 | 1989-08-08 | Polysar Limited | Microbial treatment of wastewater to remove tertiary butyl alcohol |
| CN1616358A (en) * | 2003-11-13 | 2005-05-18 | 栾兴社 | Method for preparing biological flocculant from arthrobacter |
| CN1686850A (en) * | 2005-04-08 | 2005-10-26 | 山东金佰生物技术有限公司 | Downstream extraction technique for production flocculant of microbe |
| CN1911825A (en) * | 2006-08-04 | 2007-02-14 | 哈尔滨工业大学 | Compound biological flocculant |
| CN101555054A (en) * | 2009-05-19 | 2009-10-14 | 山东齐东方投资有限公司 | Method for extracting microbial polysaccharide flocculant by membrane concentration and pneumatic drying method |
| CN101560536A (en) * | 2009-05-19 | 2009-10-21 | 山东齐东方投资有限公司 | Fermentation method of microbial polysaccharide flocculant |
| CN104109693A (en) * | 2014-07-21 | 2014-10-22 | 济南大学 | Microbial flocculant capable of treating strongly-alkaline waste water containing calcium ions and applications thereof |
Non-Patent Citations (1)
| Title |
|---|
| 栾兴社等: "微生物絮凝剂产生菌节杆菌LF-Tou2的培养和絮凝条件研究", 《现代化工》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104528952B (en) | 2016-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lee et al. | Microbial flocculation, a potentially low-cost harvesting technique for marine microalgae for the production of biodiesel | |
| CN103205412A (en) | Immobilized microbial preparation and method of treating meat product processing waste water by utilizing same | |
| Bao-jun et al. | The research status and development trend of microbial flocculant | |
| CN110157632A (en) | A kind for the treatment of of dyeing wastewater removes ammonia nitrogen composite bacteria agent with salt tolerant | |
| CN105238843B (en) | A kind of microbial flocculant, preparation method and applications | |
| CN107460144B (en) | Aerobic active marine bacterium and preparation method of decolorizing flocculant thereof | |
| CN104611279B (en) | A kind of red city Rhodococcus sp LH N13 and its microbial bacterial agent and purposes | |
| CN106746160A (en) | A kind of vancomycin produces the processing method of waste water | |
| Syafalni et al. | Production of bioflocculant by Chryseomonas luteola and its application in dye wastewater treatment | |
| CN107502569B (en) | Composite microbial inoculum for decoloring flocculation and preparation method of flocculant thereof | |
| CN111233167B (en) | Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation | |
| CN102557211B (en) | Multi-functional composite bioflocculant for wastewater treatment | |
| CN104843870A (en) | Magnetic carrier immobilized microorganism live bacteria preparation and preparation method and application thereof | |
| Wei et al. | Decolorization of dye solutions with Ruditapes philippinarum conglutination mud and the isolated bacteria | |
| CN107723265A (en) | A kind of Alteromonad and the biological flocculant prepared using the bacterium and preparation method | |
| CN114292797B (en) | Physarum viscosum and application of microbial flocculant thereof in sewage treatment | |
| CN104109693B (en) | A kind of microbial flocculant processing calcium ions strong basicity waste water and application thereof | |
| CN109019874B (en) | Biological growth promoter for papermaking wastewater and preparation method thereof | |
| CN111268805A (en) | Bacillus cereus and application thereof in pig manure biogas slurry flocculation | |
| CN104528952B (en) | A kind of effective microorganisms processing not calcium ions strong basicity waste water and application thereof | |
| CN104556405B (en) | A kind of Biological compound flocculant containing ferric ion and application thereof | |
| CN104310737A (en) | Method for realizing sludge reduction by microorganisms | |
| CN101942407B (en) | Producing strain and production method for producing microbial flocculant from wheat starch waste water | |
| CN104692522B (en) | The composite drug of pollutant benzoquinone and the method for degradation treatment Pollutants in Wastewater benzoquinone in degrading waste water | |
| CN1274805C (en) | Process for screening of microorganism flocculator producing bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160720 Termination date: 20210106 |