CN1686850A - Downstream extraction technique for production flocculant of microbe - Google Patents
Downstream extraction technique for production flocculant of microbe Download PDFInfo
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- CN1686850A CN1686850A CN 200510042280 CN200510042280A CN1686850A CN 1686850 A CN1686850 A CN 1686850A CN 200510042280 CN200510042280 CN 200510042280 CN 200510042280 A CN200510042280 A CN 200510042280A CN 1686850 A CN1686850 A CN 1686850A
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Abstract
The present invention provides a downstream extraction process of microbial flocculating agent production. Said process is characterized by that it makes the fermented liquor produced by using upstream microbial flocculating agent produced bacterium arthrobacter LF-Tou2 as production culture and making it undergo the process of fermentation procedure undergo the procedure of sterilization, concentration, purification and dehydration in the downstream so as to obtain the invented microbial flocculating agent which can be used for making water treatment. Said invention also provides its application range.
Description
One, technical field
The present invention relates to the downstream extraction technique that a kind of microbial flocculant is produced.Microbial flocculant is a kind of novel water quality treatment agent of representing the flocculation agent developing direction, and it is produced by the Institute of Micro-biology of special metabolic function, and its function shows as the removal of adsorbing, flocculate of suspended particle, gegenion etc. in the liquid system.Extraction to biological flocculant belongs to the downstream biotechnology that specific function biomacromolecule preparation is produced.
Two, background technology
The essence of microbial flocculant is biomacromolecule, but is the novel water conditioner that possesses characteristics such as nontoxic, safe in utilization, biodegradable large-scale industrial production, mainly contains polyose, glycoprotein analog, protein-based, proteoglycan and DNA class etc.Is " 200310105515.X " the inventor in the number of patent application of application in 2003, denomination of invention is removed impurity and thalline for the extracting method of the microbial flocculant reported in the patent documentation of " method that is prepared biological flocculant by Arthrobacter " relates to earlier by centrifugal or filtering method, precipitates according to the character of fermented liquid and corresponding organic solvent, the inorganic salt taked of characteristics of flocculation agent material then; Precipitations such as organic solvent, mineral acid, CPC; Adopt fractionation; Extract with mineral acid, alkali, organic solvent repeated precipitation, dissolution extraction cooperation; Adopt organic solvent, inorganic salt, low molecule alkanes post precipitation to extract with the dialysis of CPC filter membrane, the downstream extraction of fermented liquid exists all that efficient is low, cost is high and is not suitable for the deficiency of big production aspect again.
Three, summary of the invention
The purpose of this invention is on the basis of 200310105515.X, overcome the deficiency of microbial flocculant downstream extraction technique, what a kind of efficient height is provided, cost is low and is suitable for scale operation prepares the downstream production technique of biological flocculant goods by fermented liquid.This technology be with upstream bacterium for producing flocculant of microbe Arthrobacter LF-Tou2 for produce bacterial classification through the fermented liquid of fermentation procedure production again through degerming, concentrate, purifying, thickening make the water treatment microbial flocculant, the downstream extraction technique of this biological flocculant specifically adopts following steps:
A) bacterial classification of fermented liquid is removed
To be controlled at 18-30 ℃ from the temperature of the fermented liquid of upstream process, fermented liquid is stirred 1-5min under 15-37r/min, removing thalline with aperture 0.1-1.0 μ m membrane filtration, to get sterilization clear standby;
B) sterilization clear concentrates
The temperature of sterilization clear is controlled at 10-26 ℃, and the film of using aperture 5000-10000 molecular weight is at 0.2-2kg/cm
2Circulate under the pressure and hold back, concentrate the content of solid substance macromole in solution and be increased to original 2-10 doubly, must concentrate flocculation agent liquid crude product;
C) pre-treatment of crude product
Is that the NaOH aqueous solution of 0.01-0.1mol/L is handled 10-40min with crude product under 20-40r/min stirs adding concentration under the 20-40 ℃ of temperature while stirring, the pre-treatment thing is standby;
D) prepurification
The temperature of pre-treatment thing is controlled at 30-40 ℃, add anionite-exchange resin, whip attachment 2-8h leaves standstill 20-60min then, uses the water rinse removal of impurities, with pH=7.0-7.5 concentration is NaCl aqueous solution rinsing 1-3 time of 30-60mmol/L, be that the 0.7-3mol/L NaCl aqueous solution stirs rinsing 10min with concentration again, leave standstill 1h, extract the elutriant separation resin, resin concentration after the separation be 0.7-3mol/LNaCl aqueous solution wash-out once, it is standby that the elutriant that merges secondary gets prepurification liquid.
E) microbial flocculant preparation
Prepurification liquid is held back concentrated with the film of aperture 5000-10000 molecular weight, made solid content reach 3-10%, make powdery biological flocculant product with spray-dryer spraying drying under 75-90 ℃ temperature then.
The downstream extraction technique of microbial flocculant production of the present invention compare with various microorganism extracting method in the prior art have the efficient height, cost is low, be fit to large-scale industrial production.Microbial flocculant flocculation efficiency height through extract producing, nontoxic, use non-secondary pollution, biodegradable, can carry out characteristics such as large-scale promotion application, have broad application prospects.
Four, embodiment
The downstream extraction technique that microbial flocculant of the present invention produces adopts Arthrobacter LF-Tou2 to carry out fermentation culture, with the broth extraction microbial flocculant that obtains.
Embodiment 1
A) bacterial classification of fermented liquid is removed
To be controlled at 30 ℃ from the fermented liquid temperature of upstream process, under 37r/min, stir 5min, use
Aperture 0.7 μ m membrane filtration is standby except that thalline gets sterilization clear;
B) sterilization clear concentrates
The temperature of sterilization clear is controlled at 25 ℃, and the film of using aperture 10000 molecular weight is at 1kg/cm
2Circulate under the pressure and hold back, concentrate the solid substance macromole and make its content in liquid improve 8 times, must concentrate flocculation agent liquid crude product;
C) pre-treatment of crude product
Crude product under stirring, 40r/min is handled 30min at the NaOH aqueous solution with 0.1mol/L concentration under 35 ℃ of temperature, again pretreatment temperature is controlled at 40 ℃, add anionite-exchange resin whip attachment 6h, leave standstill 60min then, remove foreign protein with water rinse, with the NaCl rinsing of the 30-60mmol/L concentration of pH=7.0-7.5 3 times, the NaCl aqueous solution with 1mol/L concentration is stirring wash-out 30min again, after leaving standstill 1h, extract elutriant, the resin of draining is used 2mol/L NaCl aqueous solution wash-out once again, and the elutriant that merges secondary is standby, checks purity with gel method.
D) preparation of microbial flocculant
The purifying elutriant is held back concentrated with the film of aperture 10000 molecular weight, made solid substance content in solution reach 10%, make powdery biological flocculant finished product with spray-dryer spraying drying under 75-90 ℃ of temperature then.
Embodiment 2
To be controlled at 20 ℃ from the temperature of the fermented liquid of production upstream operation, and stir 3min under 25r/min, and remove thalline with aperture 0.2 μ m membrane filtration, the film of using aperture 6000 molecular weight again is at 0.5kg/cm
2Hold back, be concentrated into solid content to 8 times under the pressure, must concentrate flocculation agent liquid crude product.Crude product gets the pre-treatment thing at 35 ℃ of NaOH aqueous solution pre-treatment 25min under 25r/min stirs that use 0.02mol/L concentration down.The temperature of control pre-treatment thing adds anionite-exchange resin at 35 ℃, and whip attachment 6h leaves standstill 50min then, uses water rinse.Use pH=7.0-7.5, concentration is the NaCl aqueous solution rinsing secondary of 35mmol/L, be that the 0.75mol/L NaCl aqueous solution carries out static wash-out after stirring with concentration again, after leaving standstill 1h, extract elutriant, the resin of draining again with concentration be 0.80mol/L NaCl aqueous solution eluant solution once, merge elutriant, the purifying elutriant.The purifying elutriant is held back concentrated with the film of aperture 6000 molecular weight, is made the content of solid substance reach 8%, then with spray-dryer at 86 ℃ of spraying powdering biological flocculant products.Product adopts the aluminium foil vacuum packaging, and packing specifications does not wait between 1-25kg, with the listing of aqueous solution form, then adopts plastics rubber drum packing as product, and its solid content should be controlled between the 3-10%.
Can be widely used in the flocculation of liquid-phase systems such as containing particle suspensions or heavy metal by the microbial flocculant of present method production.The processing of sewage is as, the sewage of sanitary sewage, industrial sewage, food and fermentative production; The processing of supplying water as, river, river, lake water, reservoir water; The coal washing water flocculation; Power plant cooling water is softening; Middle water treatment; Bioseparation is as, single cell protein production; Mud concentrates; Ecological lake water disinfection and bioprocess technology clarification as, antibiotic fermentation liquor treatment etc.Product should store at shady and cool, dry place.
Claims (4)
1, a kind of downstream extraction technique of microbial flocculant production, it is characterized in that upstream bacterium for producing flocculant of microbe Arthrobacter LF-Tou2 for producing the fermented liquid that bacterium produces through fermentation procedure, in the downstream through degerming, concentrate, operations such as purifying, thickening make the water treatment microbial flocculant, the downstream extraction technique of this biological flocculant specifically adopts following steps:
A) bacterial classification of fermented liquid is removed
The temperature of fermented liquid is controlled at 18-30 ℃, fermented liquid is stirred 1-5min under 15-37r/min, standby with aperture 0.1-1.0 μ m membrane filtration except that thalline gets sterilization clear;
B) sterilization clear concentrates
The temperature of sterilization clear is controlled at 10-26 ℃, and the film of using aperture 5000-10000 molecular weight is at 0.2-2kg/cm
2Circulate under the pressure and hold back, concentrate the content of solid substance macromole in solution and be increased to original 2-10 doubly, must concentrate flocculation agent liquid crude product;
C) pre-treatment of crude product
Is that the NaOH aqueous solution of 0.01-0.1mol/L is handled 10-40min with crude product under 20-40r/min stirs adding concentration under the 20-40 ℃ of temperature while stirring, the pre-treatment thing is standby;
D) prepurification
The temperature of pre-treatment thing is controlled at 30-40 ℃, add anionite-exchange resin, whip attachment 2-8h leaves standstill 20-60min then, uses the water rinse removal of impurities, with pH=7.0-7.5 concentration is NaCl aqueous solution rinsing 1-3 time of 30-60mmol/L, be that the 0.7-3mol/L NaCl aqueous solution stirs rinsing 10min with concentration again, leave standstill 1h, extract the elutriant separation resin, resin concentration after the separation be 0.7-3mol/LNaCl aqueous solution wash-out once, it is standby that the elutriant that merges secondary gets prepurification liquid.
E) microbial flocculant preparation
Prepurification liquid is held back concentrated with the film of aperture 5000-10000 molecular weight, made solid content reach 3-10%, make powdery biological flocculant product with spray-dryer spraying drying under 75-90 ℃ temperature then.
2, the downstream extraction technique method of microbial flocculant production according to claim 1 is characterized in that the aperture that concentrates used film is the 6000-9000 molecular weight with the thalline of aperture 0.15-0.8 μ m membrane filtration removal fermented liquid.
3, the downstream extraction technique method of microbial flocculant production according to claim 1 is characterized in that carrying out pre-treatment with the NaOH of 0.015-0.09mol/L to concentrating crude product.
The downstream extraction technique method that 4 microbial flocculants according to claim 1 are produced adds anionite-exchange resin when it is characterized in that purifying in the pre-treatment thing, used NaCl concentration is 0.75-2.9mol/L when whip attachment 3-6h, static wash-out.
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| CN 200510042280 CN1686850A (en) | 2005-04-08 | 2005-04-08 | Downstream extraction technique for production flocculant of microbe |
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| CN 200510042280 CN1686850A (en) | 2005-04-08 | 2005-04-08 | Downstream extraction technique for production flocculant of microbe |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101327975B (en) * | 2008-07-31 | 2011-09-28 | 东北大学 | Method for preparing microorganism flocculant |
| CN102198963A (en) * | 2011-03-17 | 2011-09-28 | 夏建锋 | Composite water treatment agent special for coal washing wastewater and preparation method thereof |
| CN102203113A (en) * | 2008-10-17 | 2011-09-28 | 佩尔希维亚有限责任公司 | Clarification process |
| CN104372029A (en) * | 2014-10-27 | 2015-02-25 | 河南城建学院 | Preparation method of extracellular polymer biological adsorbent |
| CN104528952A (en) * | 2014-07-21 | 2015-04-22 | 济南大学 | Compound microbial flocculant processing strong-alkali waste water without calcium ions and application thereof |
| CN109319906A (en) * | 2018-11-20 | 2019-02-12 | 朱计平 | A kind for the treatment of method for coal washing water |
-
2005
- 2005-04-08 CN CN 200510042280 patent/CN1686850A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101327975B (en) * | 2008-07-31 | 2011-09-28 | 东北大学 | Method for preparing microorganism flocculant |
| CN102203113A (en) * | 2008-10-17 | 2011-09-28 | 佩尔希维亚有限责任公司 | Clarification process |
| CN102198963A (en) * | 2011-03-17 | 2011-09-28 | 夏建锋 | Composite water treatment agent special for coal washing wastewater and preparation method thereof |
| CN102198963B (en) * | 2011-03-17 | 2012-07-25 | 夏建锋 | Composite water treatment agent special for coal washing wastewater and preparation method thereof |
| CN104528952A (en) * | 2014-07-21 | 2015-04-22 | 济南大学 | Compound microbial flocculant processing strong-alkali waste water without calcium ions and application thereof |
| CN104372029A (en) * | 2014-10-27 | 2015-02-25 | 河南城建学院 | Preparation method of extracellular polymer biological adsorbent |
| CN109319906A (en) * | 2018-11-20 | 2019-02-12 | 朱计平 | A kind for the treatment of method for coal washing water |
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