CN104106459A - Dangling hydrophonic seedling culturing method for lily scales - Google Patents
Dangling hydrophonic seedling culturing method for lily scales Download PDFInfo
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- CN104106459A CN104106459A CN201410358151.4A CN201410358151A CN104106459A CN 104106459 A CN104106459 A CN 104106459A CN 201410358151 A CN201410358151 A CN 201410358151A CN 104106459 A CN104106459 A CN 104106459A
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- lily
- cuttage
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Abstract
The invention discloses a dangling hydrophonic seedling culturing method for lily scales. The method includes disposing a cuttage medium in a dangling hydrophonic sponge; inserting the lily scales in an activated carbon culture medium; culturing for 60-80 days under the conditions of 12-hour lighting with lighting intensity of 5500Lx in daytime, 12-hour darkening at night and at temperature of 25 DEG C; changing distilled water every week, and spraying a nutrient solution to the cuttage scales in the morning and in the evening each day. The dangling hydrophonic seedling culturing method is simple and easy in operation, wide in material source range and low in investment cost, better medium environment can be provided for the cuttage scales, rooting rate, sprouting rate, multiplication coefficient, rooting coefficient and the like are increased remarkably when compared with those of a conventional sand-culture method, and rotting rate and death rate of the scales in the process of cuttage are close to zero. The dangling hydrophonic seedling culturing method is simple, easy, convenient and easy to popularize, survival rate in cuttage of the lily scales is increased, and seedling culturing cost is lowered.
Description
Technical field
The present invention relates to the unsettled water planting seedling-cultivating method of plant asexual reproduction method, particularly a kind of lily bud scale.
Background technology
Vegetative propagation not only can keep its good moral character, can be large-scale industrialized growing seedlings simultaneously and lays the foundation.Tissue culture propagation method, technology and condition of culture requirement is at present high, not easy to operate, should not promote, and cuttage is simple to operate, and condition of culture requires lower, suitable popularization.But the survival rate of cuttage branch is not high in actual production, survival rate the nutriment of cuttage species inner accumulation and the restriction of endogenous hormones, is also subject to the impact of the extraneous factors such as temperature, humidity, illumination, gas permeability except being subject to.Cuttage branch respiration in rooting process is vigorous especially, and whether oxygen one of abundance essential condition that can be cuttings survive.For this problem, current more scholar thinks that good matrix is mixed-matrix organic and that inorganic matrix forms.But this type of matrix cost is higher and difficult acquisition, be not suitable for promoting.
Tradition water planting cuttage cultural method, the factor of most critical is the dissolved oxygen amount in water, content of oxygen dissolved in water is lower, is unfavorable for the growth of branch root root system.In order to increase dissolved oxygen in water, generally adopt bubble method, a mist method, flow liquid method or add oxygenation agent.The hot and humid rooting of cuttings that is conducive to is also beneficial to bacterial reproduction, once cutting is infected by bacterium in water planting, consequence is more serious compared with earth culture.For reduce bacterium to the infringement of cutting, improve cutting survival rate, the reply cutting sterilization processing that carries out disinfection.Visible traditional water planting will guarantee dissolved oxygen amount, and it is higher to reduce the required cost of infection, and complicated operation.The sand water-holding capacity of the husky training of tradition is lower, and is unfavorable for root system development.Because the specific surface area relative activity charcoal of sand is much smaller, adsorption capacity, also much smaller than active carbon, causes plant root cleannes lower, easily by miniature organism, is encroached on the more difficult control of moisture supply of husky training.
Lily breeding is at present domestic key subjects, and scale cuttage is main a kind of breeding practice, also more for the research of lily bud scale cuttage.At present the most frequently used is by low temperature treatment, to break the method for lily bulb dormancy, and the method can improve blade pumping rate, and pumping rate can reach 51%-61%, but lower, and complicated operation; Separately have garden mould and river sand are cultivated after proportioning by a certain percentage, but survival rate is not high.
Summary of the invention
The technical problem to be solved in the present invention is for overcoming the deficiency of existing lily cut flowers cuttage, and provides a kind of survival rate high, and low cost is simple to operate, the unsettled water planting seedling-cultivating method of lily bud scale that popularization degree is high.
Realizing technical scheme of the present invention is:
A unsettled water planting seedling-cultivating method for lily bud scale, comprises the steps:
(1) prepare in seedbed: get a rectangular sponge, sponge is cleaned with distilled water, and with 70% alcohol-pickled washing 20-30 min, with distilled water, wash away after alcohol again, long limit doubling surrounds a drum, and 2/3 place of drum sponge is tied up, and with the sponge at the place of tying up, does not stay gap to be advisable, active carbon is inserted in bottom in cavernous body top hollow region, and bottom is placed in the container that distilled water is housed;
(2) choose without damage by disease and insect, healthy lily bulb, the skin of getting bulb is loose, epidermis without the scale of disability as cuttage material;
(3) lily bud scale of step (2) is cleaned, put into bactericide and soak after 25-30 minute, then use GA
31000mg/L soaks 5-5.5 hour, pulls out, cleans, and dries;
(4) the base portion cuttage of the lily bud scale of step (3) is cultivated in the active carbon of the cavernous body of step (1); Condition of culture is daylight 12 hours, intensity of illumination 5500Lx; Evening, dark processing was 12 hours, 25 ℃ of temperature; Cultivation cycle is 60-80 days, within every 7 days, changes first water, and morning every day, evening spray nutrient solution to cuttage body respectively scale surface is drenched.
The packed height of described active carbon is 1 ~ 2cm.
Described bactericide is 1000 times of liquid of 50% carbendazol wettable powder.
Described nutrient solution is Hoagland ' s(Gram suddenly) nutrient solution, and pH is 6.0-6.2.
Easily there is the situation that scale is rotten in conventional lily bud scale cuttage, the unsettled water planting seedling-cultivating method of lily bud scale of the present invention is having new breakthrough aspect the enhancement of environment of scale root growth, by the unsettled setting of root and by sponge, can solve ventilative difficult problem; Sponge and active carbon all have water imbibition, can solve this key factor of water binding capacity, sponge in water body and the surface of active carbon can form huge biomembrane, the detergent power of increasing to water body, make the water in bottle comparatively clean, avoid plant root to be injured by germ, obviously alleviated rotting of scale, improved the survival rate of growing seedlings.
The advantage of the unsettled water planting seedling-cultivating method of lily bud scale of the present invention is: survival rate is high, and low cost is simple to operate.
Accompanying drawing explanation
Fig. 1, Fig. 2 are the plant that the unsettled water planting growing nursery and culture of the embodiment of the present invention 1 tiger lily lily goes out;
Fig. 3, Fig. 4 are the plant that the husky cultivation of tiger lily lily tradition seedling cultivates;
Fig. 5 is the plant that the unsettled water planting growing nursery and culture of the embodiment of the present invention 2 Fragrant Lily goes out;
Fig. 6 is the plant that the husky cultivation of Fragrant Lily tradition seedling cultivates.
Embodiment
Below in conjunction with embodiment, content of the present invention is further described, but is not limitation of the invention.
Embodiment 1
A unsettled water planting seedling-cultivating method for lily bud scale, take tiger lily lily as example, comprises the steps:
The collection of tiger lily lily bud scale: gather a collection ofly without damage by disease and insect, healthy tiger lily lily plant on flowers market, strip outer loose, epidermis without the lily bud scale of disability.
Matrix configuration: get a rectangular sponge, sponge is cleaned with distilled water, and with 70% alcohol-pickled washing 20 min, with distilled water, wash away after alcohol again, long limit doubling surrounds a drum, and 2/3 place of drum sponge is tied up, and with the sponge at the place of tying up, does not stay gap to be advisable, the powdered activated carbon of inserting 1 ~ 2cm height in the bottom of cavernous body top hollow region, bottom is placed in the vial that distilled water is housed.
Cuttage processing and cuttage: the tiger lily lily bud scale of choosing is cleaned with clear water, put into 1000 times of 50% carbendazim dilutions and soak 30 minutes, then use GA
31000mg/L soaks 5 hours, then after clean with distilled water flushing, dries scale surface moisture.With being about to tiger lily lily bud scale base portion, place completely in the active carbon that is contacted with unsettled cavernous body.Use unsettled cavernous body to carry powdered activated carbon and make the loose structure of sponge and active carbon easily form huge biomembrane, can strengthen water body purification ability, and the good physico chemical adsorption ability of active carbon, the moisture that plant base portion is absorbed is comparatively clean.
Daily management after cuttage: the management after cuttage is mainly daylight 12 hours, intensity of illumination 5500Lx; Evening, dark culturing was 12 hours, and temperature is controlled at 25 ℃ of left and right, within every 7 days, changed first water, and morning every day, evening are sprayed Hoagland ' s(Gram suddenly to cuttage plant) nutrient solution.
Adopt the unsettled water planting of the present invention and husky training contrast ratio pair, experimental result is in Table 1:
Table 1: tiger lily lily bud scale is cultivated 80 days experimental results
With reference to Fig. 1-4, the experiment proved that and adopt the unsettled water planting seedling-cultivating method of the present invention, tiger lily lily blade pumping rate has reached 76.67%, and the average height of sprouting is 6.10cm, and the color of leaf is dominant with green; The rooting rate of scale is about 83.33%, and the coefficient of taking root is 2.03, and value-added coefficient is 1.73, and every result is all cultivated higher than sandy soil.
Embodiment 2
A kind of unsettled water planting of lily bud scale is grown seedlings, and take Fragrant Lily as example, and method step is with embodiment 1.
Adopt the unsettled water planting of the present invention and husky training contrast ratio pair, experimental result is in Table 2:
Table 2: 60 days experimental results of Fragrant Lily scale culture
With reference to Fig. 5-6, the experiment proved that and adopt the unsettled water planting seedling-cultivating method of the present invention, Fragrant Lily scale rooting rate has reached 93.33%, and the coefficient of taking root is 2.4, and value-added coefficient is 3.23, and every result is all cultivated higher than sandy soil.
Claims (4)
1. the unsettled water planting seedling-cultivating method of lily bud scale, is characterized in that: comprise the steps:
(1) prepare in seedbed: get a rectangular sponge, sponge is cleaned with distilled water, and with 70% alcohol-pickled washing 20-30 min, with distilled water, wash away after alcohol again, long limit doubling surrounds a drum, and 2/3 place of drum sponge is tied up, and with the sponge at the place of tying up, does not stay gap to be advisable, active carbon is inserted in bottom in cavernous body top hollow region, and bottom is placed in the container that distilled water is housed;
(2) choose without damage by disease and insect, healthy lily bulb, the skin of getting bulb is loose, epidermis without the scale of disability as cuttage material;
(3) lily bud scale of step (2) is cleaned, put into bactericide and soak after 25-30 minute, then use GA
31000mg/L soaks 5-5.5 hour, pulls out, cleans, and dries;
(4) the base portion cuttage of the lily bud scale of step (3) is cultivated in the active carbon of the cavernous body of step (1); Condition of culture is daylight 12 hours, intensity of illumination 5500Lx; Evening, dark processing was 12 hours, 25 ℃ of temperature; Cultivation cycle is 60-80 days, within every 7 days, changes first water, and morning every day, evening spray nutrient solution to cuttage body respectively scale surface is drenched.
2. the unsettled water planting seedling-cultivating method of lily bud scale according to claim 1, is characterized in that: the packed height of the described active carbon of step (1) is 1 ~ 2cm.
3. the unsettled water planting seedling-cultivating method of lily bud scale according to claim 1, is characterized in that: the described bactericide of step (3) is 1000 times of liquid of 50% carbendazol wettable powder.
4. the unsettled water planting seedling-cultivating method of lily bud scale according to claim 1, is characterized in that: the described nutrient solution of step (4) is that pH is the nutrient solution of Gram suddenly of 6.0-6.2.
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CN106857206A (en) * | 2017-01-12 | 2017-06-20 | 浙江省林业科学研究院 | A kind of D. primulinum Hydroponic culture and cultural method |
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