CN104099384A - Method for producing xylaria nigripes exopolysaccharides through submerged fermentation - Google Patents
Method for producing xylaria nigripes exopolysaccharides through submerged fermentation Download PDFInfo
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Abstract
The invention discloses a method for producing xylaria nigripes exopolysaccharides through submerged fermentation. The method comprises the steps as follows: activated xylaria nigripes strains are inoculated into a seed tank for propagation, then propagated liquid seeds are inoculated into a specially-made polysaccharide-producing liquid culture medium for fermentation, and a fermentation liquid is separated to extract the exopolysaccharides. According to the method, research is conducted to solve the technical problems encountered during amplification from small tests to industrial production, on the premises that a fermentation culture medium (especially a carbon source) is continuously optimized through small and medium tests and the biomass and the exopolysaccharides are increased, the problems of fermentation process parameter control, extracting equipment model selection, optimization and the like during enlargement are solved, the strains are successfully inoculated into fermentation tanks of 10-20 t finally for industrial production, the high-quality xylaria nigripes exopolysaccharides are produced smoothly, and the continuous, automatic and industrial production is actually realized.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of method that crow spirit ginseng exocellular polysaccharide is produced in deep layer liquid state fermentation.
Background technology
Wu Lingcan belongs to the Xylaria nigripes of Ascomycetes, Xylariaceae, it is a traditional medicinal fungi, formal name used at school Xylaria nigripes, another name Wu Lingcan, vertical (clump) egg of chicken, have another name called shake thunder, be grown in the black wing soil of deep under ground and dwell on the discarded ant nest of termite, by the research of world's medicinal fungi, Application Areas, be all considered as rare, rare medicinal herbs, there is pharmaceutical use widely.
Wu Lingcan be grown in face south massif hillside fields warm or the discarded fungus garden of termite of river levee slight slope on, be by Xylaria nigripes mycelia densely covered on fungus garden, in its growth later stage, the intensive sclerotium forming of part, is commonly called as " Wu Lingcan ", heavily approximately 5-30 grams, spherical, class is spherical or irregular flat strips is block, 2-12 centimetres of diameters, appearance brown or black smooth or wrinkle more, there is shoestring (rhizoid) one end or has umbilication, inner white, meat, hard or soft, the sweet perfume (or spice) of taste.Sclerotium upper end has handle to poise in fungus garden upper wall and with shoestring to be connected.June in next year is to October, and when fungus garden, around soil temperature is 20 when above (at a time when this thunderstorm and autumn rain season), and sclerotium is sprouted mycelia, at earth's surface formation stroma, is commonly called as " charcoal rod ", is scattered or all living creatures on earth's surface.Wild crow spirit ginseng mainly originates in the ground such as Sichuan, Yunnan, Guangdong, and also there is growth in Zhejiang, Jiangxi, Jiangsu, the Wu Ling ginseng place of production take the Guanxian county in Sichuan, very the ground such as shoulder as top grade.Because Wu Lingcan grows in the soil of underground 2 meters-20 meters, dwell on the discarded ant nest of termite, digging is very difficult, therefore natural resources is very rare.Wu Lingcan is medicinal fungi and edible mushrooms, has better nutritivity and pharmaceutical use, is comparatively ideal health care food (medicine) product.
Wu Lingcan, warm in nature, taste is sweet, have that tonifying Qi is reinforced the kidney, an effect of invigorating the spleen dehumidifying, tranquilizing and allaying excitement, cure mainly insufficiency of the spleen few food, postpartum and postoperative severe loss of blood, postpartum breast less, gastroptosis, hernia, palpitation and insomnia, infantile convulsion and wound etc.Experimental study shows; the main composition of Wu Lingcan is polysaccharide; its Crude polysaccharides not only has the body's immunity of raising and anti-aging effects; and on mouse broken end ischemic, the time lengthening impact test of panting shows through Chinese medical courses in general institute institute of materia medica; Wu Ling ginseng Crude polysaccharides has obvious cerebral protection, and the dysmnesia that electroshock is caused also have provide protection.China Concord Medical Science University basis tested showing, Wu Ling ginseng Crude polysaccharides can obviously suppress activity of monoamine oxidase in mouse brain, thereby improves central neurotransmitter function.
Because Wu Lingcan grows in the soil of underground 2 meters-20 meters, dwell on the discarded ant nest of termite, digging is very difficult, therefore natural resources is very rare.At present domestic only have minority universities and colleges to launch the research that crow spirit ginseng is produced in deep layer liquid state fermentation, and the overwhelming majority is all confined to lab scale level, be all the mycelium that obtains crow spirit ginseng be that major objective is launched research, and at present domestic functional component polysaccharide of joining about the spirit of liquid state fermentation production crow does not almost have relevant report, how more not research and solve and from lab scale grafting, to be amplified to the culture medium prescription that industrialization runs into producing and to choose, fermentation operation parameter is controlled, the technical barriers such as the research of Polysaccharide from Bee, so at present domestic producer that does not also have real industrialization liquid state fermentation to produce black clever gracilis polysaccharide.
Summary of the invention
The object of the invention is in order to solve the technical bottleneck of the clever gracilis polysaccharide of commercial scale production crow, adopt this invention can be continuously, automatization, industrialization produce the clever gracilis polysaccharide of high-quality crow.
To achieve these goals, the present invention mainly carries out according to following step:
The method that crow spirit ginseng exocellular polysaccharide is produced in industrialization deep layer liquid state fermentation of the present invention comprises the steps:
A kind of method of preparing crow spirit ginseng fermented liquid, comprise the steps: that the liquid fermentation medium after crow spirit ginseng seed culture fluid access sterilizing is carried out to deep layer liquid state fermentation obtains Wu Ling ginseng fermented liquid, the inoculum size of described deep layer liquid state fermentation is 10～20%, ventilating ratio is 1:0.3-1.0vvm, tank pressure 0.02～0.05Mpa, at 22～30 ℃, cultivate 3～5 days; Described liquid fermentation medium formula is: the high temperature resistant α-amylase 0.001-0.007% (v/v) of glucose 1-3%, starch 1-3%, peptone 0.1-0.5% (v/v), soybean cake powder 1-3%, potassium primary phosphate 0.1-0.5%, magnesium sulfate heptahydrate 0.1-0.5%, zinc sulfate 0.1-0.5%, defoamer 0.01-0.05%, 20000u/ml, regulates pH value 5.0-6.5." % " during above substratum forms except high temperature resistant α-amylase all represents g/100ml.
Described defoamer can be the materials such as vegetables oil (soya-bean oil, rapeseed oil) class, organic ethers.
Described crow spirit ginseng seed culture fluid is cultivated acquisition by secondary, and concrete grammar is as follows:
1) first order seed is cultivated: by the liquid seed culture medium after activated shaking flask bacterial classification access sterilizing, inoculum size is 0.1～2% (v/v), ventilating ratio be 1:0.3 ?0.7vvm, tank pressure 0.02～0.05Mpa, at 22～30 ℃, cultivate 3～5 days, obtain Wu Ling ginseng first order seed nutrient solution, standby;
2) secondary seed cultivation: by the step 1) liquid seed culture medium after the first order seed nutrient solution access sterilizing of gained, inoculum size is 10～20% (v/v), ventilating ratio be 1:0.3 ?1.0vvm, tank pressure 0.02～0.05Mpa, at 22～30 ℃, cultivate 2～4 days, obtain Wu Ling ginseng secondary seed nutrient solution;
Wherein said liquid seed culture medium substratum is: glucose 2-4%, peptone 0.1-0.5%, soybean cake powder 1-3%, potassium primary phosphate 0.1-0.5%, magnesium sulfate heptahydrate 0.1-0.5%, zinc sulfate 0.1-0.5%, defoamer 0.01-0.05%, regulate pH value 5.0-6.5.The volume of first class seed pot is 100-500L, and the volume of secondary seed tank is 1000-5000L, and loadings is 70-90% (v/v)." % " during above substratum forms all represents g/100ml.
The fermentor tank volume of described deep layer liquid state fermentation is 10000-20000L, and loadings is 70-90% (v/v).
The endpoint standard of described deep layer liquid state fermentation: fermented liquid is peat-reek, bacterium ball rolls up, filtrate is muddy; Reducing sugar is down to minimum and slightly fluctuation, and reducing sugar is controlled at below 0.5% (g/100g); Microscopy mycelia is in small, broken bits, without living contaminants.
An extracting method for crow spirit ginseng exocellular polysaccharide, comprises the steps:
1) separation: the crow spirit ginseng separation of fermentative broth by above-mentioned gained, obtains Wu Ling ginseng filtrate;
2) concentrated: by step 1) filtrate of gained adopts secondary cryoconcentration, and obtaining concentrated proportion is the thickened pulp of 1.25～1.40 (50 ℃ of heat are surveyed);
3) alcohol precipitation, oven dry: 95% alcohol that adds thickened pulp weight 3-5 doubly to measure, with Ebullioscope, detect the about 65-80% of mixed solution alcoholic strength (v/v), standing 8-16h, carries out vacuum drying by throw out, temperature 50-75 ℃, vacuum tightness is not less than 0.085MPa; Drying to being dried thing moisture content is 3～8% (g/100g), obtains the outer sugar of born of the same parents.
Wherein, step 1) described separation adopts horizontal screw centrifuge to carry out, rotating speed be controlled at 2500 ?3500r/min.
Step 2) described secondary cryoconcentration is: one-level adopt 1 ?3t double-effect evaporator concentrated, by fermented liquid be concentrated to proportion be 1.15 ?1.20 (50 ℃ heat survey), secondary adopt 1 ?3t haplo-effect concentrator concentrated, be about to proportion and be 1.1 ?the concentrated solution of 1.20 (50 ℃ of heat are surveyed) continue to be concentrated to the thickened pulp that proportion is 1.25～1.40 (50 ℃ of heat are surveyed); Concentration process all requires to control temperature not higher than 75 ℃, and vacuum tightness is not less than 0.085MPa.
In the present invention alcohol precipitation be adopt 1 ?3t Alcohol-settling tank, alcohol precipitation number of times is more, the content of the outer sugar of born of the same parents is higher.The vacuum drying oven that vacuum drying adopts, the outer sugared content of born of the same parents that the present invention extracts 20 ?50%.
The present invention has advantages of following outstanding:
For being amplified to by lab scale, the technical barrier that runs in suitability for industrialized production studies just in the present invention, at lab scale and pilot scale, continue to optimize fermention medium (especially carbon source), promote on the basis of biomass and exocellular polysaccharide, the difficult problems such as the Fermentation Process of Parameter control that runs in amplification process, extraction equipment type selecting, optimization have been solved, final successful grafting to 10 ?20t fermentor tank carry out industrialization production, and produce smoothly high-quality crow spirit ginseng exocellular polysaccharide, really realized continuously, the production of automatization, industrialization.
In order to improve the output of object product exocellular polysaccharide, the present invention is optimized and combines in the carbon source of fermention medium: the compound prescription that adopts fugitive glucose and long-acting starch.Earlier fermentation is with growth mycelium, and it is main improving biological content, so in substratum, has added the glucose carbon source that thalline is preferentially selected, rapidly propagation; The fermentation later stage will be improved the content of object product, so carbon source provides thalline relatively to utilize slower starch, and in substratum, adds appropriate amount of starch enzyme, controls thalline quantity in suitable scale, the outer sugar of the raw metabolite born of the same parents of not stopping pregnancy.
For commercial scale production provides practicable technical scheme, solved by lab scale and be amplified to the technical barrier running in suitability for industrialized production.
For born of the same parents are provided the output of outer sugar, the present invention optimizes the formula of fermention medium, for carbon source, has partly adopted compound prescription, has both guaranteed the biomass of thalline in early stage, guarantees again the output of later stage meta-bolites;
Adopt the present invention to make the high-quality polysaccharide of different content according to the market requirement and product standard, polysaccharide content can 20 ?50%.
Embodiment
By specific embodiment, further illustrate the present invention below.But the detail of embodiment only, for explaining the present invention, should not be construed as limited overall technical solution.
The crow spirit ginseng bacterium that following examples are used is bought in institute of microbiology of Chinese Academy of Sciences common micro-organisms center, bacterial classification coding CGMCC NO.1038, and take this strain bacterium is example, describes concrete scheme of the present invention in detail.But the inventive method is common to all crow spirit ginseng bacterium, is not limited in this bacterial strain.
Embodiment 1
Industrialization deep layer liquid state fermentation of the present invention is produced the method for crow spirit ginseng exocellular polysaccharide and is carried out in the steps below:
1, the preparation of Wu Ling ginseng fermented liquid: the crow spirit ginseng shaking flask bacterial strain having activated is inoculated in to seed culture medium (300L) by inoculum size 1%, wherein seed culture medium consists of glucose 2%, peptone 0.2%, soybean cake powder 1%, potassium primary phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, zinc sulfate 0.10%, soya-bean oil 0.02%, regulate pH value 5.5, ventilating ratio is 1:0.3vvm, tank pressure 0.03Mpa, 26～28 ℃ of shaking culture 4 days are get Wu Ling ginseng primary seed solution; Primary seed solution is accessed to secondary seed medium (3000L) by inoculum size 15%, wherein secondary seed medium consists of glucose 2%, peptone 0.2%, soybean cake powder 1%, potassium primary phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, zinc sulfate 0.10%, defoamer 0.02%, regulate pH value 5.5, ventilating ratio is 1:0.3vvm, tank pressure 0.03Mpa, cultivating for 26～28 ℃ 3 days is get Wu Ling ginseng secondary seed solution; Secondary seed solution is accessed to fermention medium (7500L) by inoculum size 18%, wherein fermention medium consists of glucose 2%, starch 2%, peptone 0.3%, soybean cake powder 1.5%, potassium primary phosphate 0.2%, magnesium sulfate heptahydrate 0.2%, zinc sulfate 0.10%, soya-bean oil 0.03%, Nai Gaowen α ?amylase (Zaozhuang biological company limited of outstanding promise enzyme, 20000u/ml) 0.002% (v/v), regulates pH value 5.5; Ventilating ratio is 1:0.5vvm, and tank pressure 0.03Mpa cultivates 4 days for 26～28 ℃, ferments to fermented liquid and has peat-reek, bacterium ball to roll up, and filtrate is muddy; Reducing sugar is down to minimum 0.3% also slightly fluctuation; Microscopy mycelia is in small, broken bits, and without living contaminants, get Wu Ling joins fermented liquid 8850Kg.
2, Wu Ling ginseng separation of fermentative broth: above-mentioned crow spirit ginseng fermented liquid 8850Kg is carried out to separation by horizontal screw centrifuge, rotating speed is controlled at 3000r/min, disengaging time is at 2h, obtain the wet mycelium (water content is 80%) of Wu Ling ginseng filtrate 8000Kg and 660Kg, wet mycelium directly cryodrying pulverizing makes fermentation crow spirit ginseng bacterium powder.
4, secondary cryoconcentration: the crow spirit of previous step gained is joined to filtrate 8000Kg vacuum transfer to 3t double-effect evaporator, vacuum tightness Wei ?0.085MPa, thickening temperature is under 65 ℃ of conditions, to be concentrated into proportion (the 50 ℃ of heat are surveyed) concentrated solution that is 1.20, again by concentrated solution vacuum transfer to 1.5t single-action ball-type thickener, in vacuum tightness, be 0.09MPa, thickening temperature is under 60 ℃ of conditions, to continue to be concentrated into the thickened pulp 280kg that proportion is 1.30 (50 ℃ of heat are surveyed).
5, alcohol precipitation, oven dry: to filling 95% alcohol that adds weight 1000kg in the Alcohol-settling tank of 280kg thickened pulp, with Ebullioscope, detect mixed solution alcoholic strength 72% (v/v), standing 12h, dries the separation of bottom settlings thing with vacuum drying oven, temperature 60 C, vacuum tightness 0.09MPa; Dry 15h and obtain the outer sugared 100kg of born of the same parents.Detecting moisture content is 3.5%, and polysaccharide content is 33%.
Embodiment 2
1, the preparation of Wu Ling ginseng fermented liquid: the crow spirit ginseng shaking flask bacterial strain having activated is inoculated in to seed culture medium (300L) by inoculum size 1.5%, wherein seed culture medium consists of glucose 2%, peptone 0.2%, yeast powder 1%, potassium primary phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, zinc sulfate 0.10%, rapeseed oil 0.02%, regulate pH value 5.5, ventilating ratio is 1:0.3vvm, tank pressure 0.03Mpa, 26～28 ℃ of shaking culture 3 days are get Wu Ling ginseng primary seed solution; Primary seed solution is accessed to secondary seed medium (3000L) by inoculum size 15%, wherein secondary seed medium consists of glucose 2%, peptone 0.2%, soybean cake powder 1%, potassium primary phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, zinc sulfate 0.10%, defoamer 0.02%, regulate pH value 5.5, ventilating ratio is 1:0.3vvm, tank pressure 0.03Mpa, cultivating for 26～28 ℃ 3 days is get Wu Ling ginseng secondary seed solution; Secondary seed solution is accessed to fermention medium (15000L) by inoculum size 18%, wherein fermention medium consists of glucose 1.8%, starch 2.2%, peptone 0.3%, soybean cake powder 2%, potassium primary phosphate 0.2%, magnesium sulfate heptahydrate 0.2%, zinc sulfate 0.10%, rapeseed oil 0.03%, Nai Gaowen α ?amylase (Zaozhuang biological company limited of outstanding promise enzyme, 20000u/ml) 0.002% (v/v), regulates pH value 5.8; Ventilating ratio is 1:0.3vvm, and tank pressure 0.04Mpa cultivates 4 days for 26～28 ℃, ferments to fermented liquid perfume (or spice) and has peat-reek, bacterium ball to roll up, and filtrate is muddy; Reducing sugar is down to minimum 0.4% also slightly fluctuation; Microscopy mycelia is in small, broken bits, and without living contaminants, both get Wu Ling joined fermented liquid 17700Kg.
2, the separation of Wu Ling ginseng fermented liquid: above-mentioned crow spirit ginseng fermented liquid 17700Kg is carried out to separation by horizontal screw centrifuge, obtain the wet mycelium (water content is 77%) of Wu Ling ginseng filtrate 16250Kg and 1180Kg, wet mycelium directly cryodrying pulverizing makes fermentation crow spirit ginseng bacterium powder.Concrete technology condition is: rotating speed is controlled at 3200r/min, and disengaging time is at 4.5h.
4, secondary cryoconcentration: the crow spirit of previous step gained is joined to filtrate 16250Kg vacuum transfer to 3t double-effect evaporator, vacuum tightness Wei ?0.09MPa, thickening temperature is under 63 ℃ of conditions, to be concentrated into proportion (the 50 ℃ of heat are surveyed) concentrated solution that is 1.18, again by concentrated solution vacuum transfer to 1.5t single-action ball-type thickener, in vacuum tightness, be 0.09MPa, thickening temperature is under 65 ℃ of conditions, to be concentrated into the thickened pulp 500kg that proportion is 1.35 (50 ℃ of heat are surveyed).
5, alcohol precipitation, oven dry: to filling 95% alcohol that adds weight 2200kg in the Alcohol-settling tank of 500kg thickened pulp, with Ebullioscope, detect mixed solution alcoholic strength 70% (v/v), standing 10h, dries the separation of bottom settlings thing with vacuum drying oven, 65 ℃ of temperature, vacuum tightness 0.09MPa; Dry 18h and obtain the outer sugared 210kg of born of the same parents.Detecting moisture content is 4.5%, and polysaccharide content is 30%.
Embodiment 3
For the compound prescription of fugitive glucose and long-acting starch in better checking the present invention, in industrialization, produce the superiority of black clever gracilis polysaccharide, done again several simultaneous tests.In test, only adjusted carbon source glucose in liquid fermentation medium or (with) consumption of starch, in comparative example 1, do not contain starch, glucose concn is 1.5%; In comparative example 2, do not contain glucose, starch concentration is 2.5%, and in comparative example 3, glucose content is 2.5%, and starch content is 0.5%; All the other conditions are same the present invention all, and test-results is in Table 1:
Table 1
Claims (7)
1. prepare the method that fermented liquid is joined in crow spirit for one kind, it is characterized in that the method comprises the steps: that the liquid fermentation medium after crow spirit ginseng seed culture fluid access sterilizing is carried out to deep layer liquid state fermentation obtains Wu Ling ginseng fermented liquid, the inoculum size of described deep layer liquid state fermentation is 10～20% (v/v), ventilating ratio is 1:0.3-1.0vvm, tank pressure 0.02～0.05Mpa, at 22～30 ℃, cultivate 3～5 days; Described liquid fermentation medium formula is: the high temperature resistant α-amylase 0.001-0.007% (v/v) of glucose 1-3%, starch 1-3%, peptone 0.1-0.5%, soybean cake powder 1-3%, potassium primary phosphate 0.1-0.5%, magnesium sulfate heptahydrate 0.1-0.5%, zinc sulfate 0.1-0.5%, defoamer 0.01-0.05%, 20000u/ml, regulates pH value 5.0-6.5.
2. the method for preparing crow spirit ginseng fermented liquid according to claim 1, is characterized in that described crow spirit ginseng seed culture fluid cultivates acquisition by secondary, and concrete grammar is as follows:
1) first order seed is cultivated: by the liquid seed culture medium after activated shaking flask bacterial classification access sterilizing, inoculum size is 0.1～3% (v/v), ventilating ratio be 1:0.3 ?0.7vvm, tank pressure 0.02～0.05Mpa, at 20～30 ℃, cultivate 3～5 days, obtain Wu Ling ginseng first order seed nutrient solution;
2) secondary seed cultivation: by the step 1) liquid seed culture medium after the first order seed nutrient solution access sterilizing of gained, inoculum size is 10～20% (v/v), ventilating ratio be 1:0.3 ?1.0vvm, tank pressure 0.02～0.05Mpa, at 22～30 ℃, cultivate 2～4 days, obtain Wu Ling ginseng secondary seed nutrient solution.
3. the method for preparing crow spirit ginseng fermented liquid according to claim 2, it is characterized in that described liquid seed culture medium is: glucose 2-4%, peptone 0.1-0.5%, soybean cake powder 1-3%, potassium primary phosphate 0.1-0.5%, magnesium sulfate heptahydrate 0.1-0.5%, zinc sulfate 0.1-0.5%, defoamer 0.01-0.05%, regulate pH value 5.0-6.5.
4. a kind of crow spirit ginseng fermented liquid method of preparing according to claim 1, is characterized in that the terminal of described deep layer liquid state fermentation is: fermented liquid peat-reek, bacterium ball roll up, and filtrate is muddy; Reducing sugar is down to minimum and slightly fluctuation, and reducing sugar requires below 0.5% (g/100g); Microscopy mycelia is in small, broken bits, without living contaminants.
5. an extracting method for crow spirit ginseng exocellular polysaccharide, is characterized in that the method comprises the steps:
1) separation: the crow spirit ginseng fermented liquid of claim 1 gained, through separation, is obtained to Wu Ling ginseng supernatant liquor;
2) concentrated: by step 1) filtrate of gained adopts secondary cryoconcentration, obtains the thickened pulp that concentrated proportion is 1.25～1.40;
3) alcohol precipitation, oven dry: 95% alcohol that adds thickened pulp weight 3-5 doubly to measure, with Ebullioscope, detecting mixed solution alcoholic strength is 65-80% (v/v), standing 8-16h, carries out vacuum drying by throw out, temperature 50-75 ℃, vacuum tightness is not less than 0.085MPa; Drying to being dried thing moisture content is 3～8% (g/100g), obtains the outer sugar of born of the same parents.
6. the extracting method of crow spirit ginseng exocellular polysaccharide according to claim 5, is characterized in that step 1) described in separation adopt horizontal screw centrifuge to carry out, rotating speed be controlled at 2500 ?3500r/min.
7. the extracting method of exocellular polysaccharide is joined in crow spirit according to claim 5, it is characterized in that step 2) described in secondary cryoconcentration be: one-level adopts double-effect evaporator concentrated, by fermented liquid be concentrated to proportion be 1.15 ?1.20, secondary adopts haplo-effect concentrator concentrated, be about to proportion and be 1.1 ?1.20 concentrated solution to continue to be concentrated to proportion be 1.25～1.40 thickened pulp; Concentration process all requires to control temperature not higher than 75 ℃, and vacuum tightness is not less than 0.085MPa.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997609A (en) * | 2019-01-25 | 2019-07-12 | 浙江省农业科学院 | A kind of solid medium and its artificial culture method of Wu Ling ginseng fructification |
| CN112006949A (en) * | 2020-08-23 | 2020-12-01 | 博雅(广州)生物科技研究院有限公司 | Eye repair composition and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1053264A (en) * | 1990-01-12 | 1991-07-24 | 浙江省中药研究所 | Deep fermentation technology for wuling ginseng |
-
2014
- 2014-07-15 CN CN201410336263.XA patent/CN104099384B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1053264A (en) * | 1990-01-12 | 1991-07-24 | 浙江省中药研究所 | Deep fermentation technology for wuling ginseng |
Non-Patent Citations (2)
| Title |
|---|
| 杨兴仓等: "乌灵参的红外光谱分析", 《光散射学报》 * |
| 王嘉麟等: "乌灵参的药理作用及应用进展", 《环球中医药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997609A (en) * | 2019-01-25 | 2019-07-12 | 浙江省农业科学院 | A kind of solid medium and its artificial culture method of Wu Ling ginseng fructification |
| CN112006949A (en) * | 2020-08-23 | 2020-12-01 | 博雅(广州)生物科技研究院有限公司 | Eye repair composition and application thereof |
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