CN1053264A - Deep fermentation technology for wuling ginseng - Google Patents
Deep fermentation technology for wuling ginseng Download PDFInfo
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- CN1053264A CN1053264A CN 90100231 CN90100231A CN1053264A CN 1053264 A CN1053264 A CN 1053264A CN 90100231 CN90100231 CN 90100231 CN 90100231 A CN90100231 A CN 90100231A CN 1053264 A CN1053264 A CN 1053264A
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Abstract
Wu Lingcan is a kind of medicinal fungi of preciousness, is used for the treatment of the Chinese medicinal materials of disease and health care, and the natural resource rareness can not satisfy the needs of medical and health cause.The present invention uses biotechnology, by the method for manually carrying out submerged fermentation on substratum, turns out and natural on all four crow spirit ginseng, and can carry out large-scale industrial production, satisfies the needs of medical and health cause.
Description
Wu Lingcan (Xylaria nigripes(Klotzsch) Sau) is a kind of medicinal fungi of preciousness,, improves immunologic function, promote growth, improve oxyphorase and reduce urea nitrogen content and function such as antitumor especially for treatment hepatitis.
Wu Lingcan is a kind of wild medicinal fungi, and is evident in efficacy to treatment hepatitis.Extract has anti-tumor activity, and strengthen cell and humoral immune reaction, and can promote to grow, rising oxyphorase, reduction serum urea nitrogen, its good effect, and without any side effects.The medicine such as the ribavirin that are used for the treatment of now hepatitis both at home and abroad, Ribavirina, Ah azoles's nucleosides, vidarabine, Zoviraxs etc. have certain toxicity to human body, may cause monster, suppress immunity system, to acute and the chronic viral hepatitis B curative effect is not obvious.Be wild as medicinal Wu Lingcan, natural resources is very rare, costs an arm and a leg, and has not both had artificial culture at present, does not also have the artificial fermentation, still can not satisfy the needs of society to preventing and curing diseases.
The objective of the invention is to use bionic technology,, develop the specific medicament crow spirit ginseng of control viral hepatitis, carry out large-scale industrial production, satisfy the needs of society preventing and curing diseases by the method for artificial deep layer fermenting process.
1, solid culture: culture medium prescription is glucose 20～25 grams, potato 200 grams, agar 18～20 grams, 1 liter in water.Culture condition is 24～26 ℃ of temperature, 4～6 days time.On solid medium, Wu Ling ginseng mycelium culture two days at first grows the living mycelia of the very thin greyish white color base of one deck on the inclined-plane, cultivated through 2～4 days again, on the living mycelia of base, grow the luxuriant aerial mycelium of one deck white, therefore on solid medium, obviously be divided into two-layer mycelia.The mycelia tool divides skill to separate, and diameter is about 6～15 μ, and growth pattern is two qi branch skills.
2, liquid culture: substratum is glucose 20～30 grams, Semen Maydis powder 15～25 grams, analysis for soybean powder 10～15 gram sal epsom 0.3～0.6 gram, PH5.0～6.30,1000 milliliters in water, culture condition are 24～26 ℃ of temperature, rotating speed 100～150rpm, 10～20/1000 milliliters of the solid vaccination pieces that inoculum size is 2～3 square millimeters, 4～5 days time, substratum footpath 1～1.2 kilograms per centimeter
2Sterilized 20～30 minutes, solid spawn is inserted in the cooling back, put 24～26 ℃ of temperature, rotating speed is to cultivate in the vibrator of 100～150rpm, after 1～2 day, occur a small amount of bacterium ball in the nutrient solution, diameter is at 0.2～0.4 millimeter, again after cultivating in 2～3 days, bacterium ball density increases gradually in the nutrient solution, and diameter is also in 0.2～0.4 millimeter, and the nutrient solution color becomes clear And by muddiness gradually has a small amount of byssaceous mycelium to occur, cultivate always and be full of bacterium ball and byssaceous mycelium in the nutrient solution, liquid becomes to be infused transparent ， And clearly and gives out a kind of special fragrance, at this moment, mycelial recovery rate is 1.0～1.2%.
3, fermentation, culture medium prescription are glucose 20～30 grams, Semen Maydis powder 15～25 grams, analysis for soybean powder 10～15 grams, sal epsom 0.3～0.6 gram, PH5.0～6.30,1000 millimeters in water, defoamer 0.005～0.006%.Culture condition: 24～26 ℃ of temperature, inoculum size 5～10%, tank pressure 0.3～0.5 kilograms per centimeter
2, 4～5 days time, rotating speed early stage 0～50rpm, later stage 0～150rpm, air flow be in earlier stage 0.31～0.42 liter/rise branch, 0.42～0.51 liter of later stage/rise branch.Substratum footpath steam 1～1.2 kilograms per centimeter
2Sterilized 20～30 minutes, 5～10% the bacterial classification that liquid spawn is a fermented liquid is inserted in cooling back, cultivate by above-mentioned culture condition, be full of diameter early stage in the nutrient solution and be 0.2～0.4 millimeter bacterium ball, the liquid color muddiness, the bacterium Qiu ， And that is full of diameter in the later stage nutrient solution and is 0.2～0.4 millimeter occurs with a spot of byssaceous mycelium, it is clear that nutrient solution becomes, and And gives out Wu Ling and joins distinctive fragrance from vapor pipe.
Wu Lingcan is born in underground 2～3 meters dark destroyed termitariums, thus very precious at occurring in nature, and output is few, far can not satisfy the demand of society.The present invention has realized plant size production by the method for submerged fermentation, can obtain a large amount of hyphostoma of wuling ginseng in the short period of time.In addition, the raw material of production usefulness is comparatively cheap, and production unit is a general-purpose equipment also, has less investment, instant effect, output height, advantages such as low price.
Embodiment:
MBF-III-10 multiparameter glass the fermentor tank that fermentation equipment is made for the Shanghai Huadong Chemical College.Culture medium prescription is glucose 20～30 grams, Semen Maydis powder 15～25 grams, analysis for soybean powder 10～15 grams, sal epsom 0.3～0.6 gram, PH5.0～6.30,1000 milliliters in water, defoamer 0.005～0.006%.Culture condition is 24～26 ℃ of temperature, inoculum size 5～10%, tank pressure 0.3～0.5 kg/cm, 4～5 days time, rotating speed early stage 0～50rpm, later stage 0～150rpm, 0.31～0.42 liter of air flow early stage/rise branch, 0.42～0.51 liter of later stage/rise branch.By 60～70% of the cubic capacity of the fermentor tank substratum of packing into, after steam 1～1.2 kg/cm was sterilized 20～30 fens, to be cooled during to 24～26 ℃, press 5～10% of nutrient solution volume and insert liquid spawn, tank pressure is kept 0.3～0.5 kg/cm, and cultivate premenstruum, and the bacterium nodule number in the nutrient solution increases And gradually and occurs with a spot of byssaceous mycelium, and it is clear that the color of liquid also becomes gradually.In the later stage cultivation, bacterium ball and cotton-shaped mycelium roll up, and are full of whole nutrient solution, make nutrient solution in the pasty state, and And gives out Wu Ling and joins distinctive fragrance, at this moment sampling and measuring total reducing sugar and amino nitrogen.Put a jar filtration mycelium, mycelium is put 80 ℃ of oven dry and is got final product.
Claims (6)
1, a kind of deep fermentation technology for wuling ginseng is characterized in that manually using bionic technology, and through solid culture, liquid culture is fermented, and puts jar in fermentor tank, and methods such as filtration, oven dry are turned out and natural similar Wu Lingcan.
2, zymotechnique according to claim 1 is characterized in that the solid culture based formulas is glucose 20～25 grams, potato 200 grams, agar 18～20 grams, 1000 milliliters in water, 24～26 ℃ of temperature.
3, zymotechnique according to claim 1 is characterized in that the liquid culture based formulas is glucose 20～30 grams, Semen Maydis powder 15～25 grams, analysis for soybean powder 10～15 grams, sal epsom 0.3～0.6 gram, PH5.0～6.3,1000 milliliters in water.
4,, it is characterized in that the liquid culture condition is 24～26 ℃ of temperature, rotating speed 100～150rpm according to claim 1 or 3 described zymotechniques.
5, zymotechnique according to claim 1 is characterized in that fermentation condition is 24～26 ℃ of temperature, rotating speed 0～50rpm in early stage, later stage 0～150rpm, 0.31～0.42 liter of air flow early stage/rise branch, 0.42～0.51 liter of later stage/rise branch, 0.3～0.5 liter/centimetre of tank pressure
2
6, zymotechnique according to claim 1 or 5 is characterized in that inoculum size is 5～10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90100231 CN1053264A (en) | 1990-01-12 | 1990-01-12 | Deep fermentation technology for wuling ginseng |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90100231 CN1053264A (en) | 1990-01-12 | 1990-01-12 | Deep fermentation technology for wuling ginseng |
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| Publication Number | Publication Date |
|---|---|
| CN1053264A true CN1053264A (en) | 1991-07-24 |
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| CN 90100231 Pending CN1053264A (en) | 1990-01-12 | 1990-01-12 | Deep fermentation technology for wuling ginseng |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308350C (en) * | 2004-07-28 | 2007-04-04 | 武汉大学 | Process for preparing tuckahoe mycelium extracellular polysacchride with anti-tumor activity |
| CN103333150A (en) * | 2012-12-17 | 2013-10-02 | 浙江佐力药业股份有限公司 | A preparation method for 5-methylmellein |
| CN104099384A (en) * | 2014-07-15 | 2014-10-15 | 江苏神华药业有限公司 | Method for producing xylaria nigripes exopolysaccharides through submerged fermentation |
| TWI481709B (en) * | 2014-01-07 | 2015-04-21 |
-
1990
- 1990-01-12 CN CN 90100231 patent/CN1053264A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308350C (en) * | 2004-07-28 | 2007-04-04 | 武汉大学 | Process for preparing tuckahoe mycelium extracellular polysacchride with anti-tumor activity |
| CN103333150A (en) * | 2012-12-17 | 2013-10-02 | 浙江佐力药业股份有限公司 | A preparation method for 5-methylmellein |
| CN103333150B (en) * | 2012-12-17 | 2015-09-16 | 浙江佐力药业股份有限公司 | The preparation method of 5-methyl mellein |
| TWI481709B (en) * | 2014-01-07 | 2015-04-21 | ||
| CN104099384A (en) * | 2014-07-15 | 2014-10-15 | 江苏神华药业有限公司 | Method for producing xylaria nigripes exopolysaccharides through submerged fermentation |
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