CN104096024A - Chinese medicinal composition, preparation method and application thereof - Google Patents

Abstract

Belonging to the technical field of traditional Chinese medicines and natural drugs, the invention specifically relates to a Chinese medicinal composition, a preparation method and application thereof. The Chinese medicinal composition is characterized by being prepared from the following Chinese herbal medicines by weight: 10-40 parts of poria, 10-40 parts of pinellia ternata, 10-40 parts of radix bupleuri, 10-40 parts of Fructus aurantii Immaturus, 10-40 parts of Radix Paeoniae Alba, 10-40 parts of Magnolia officinalis, 6-24 parts of licorice, 10-40 parts of gardenia, and 10-40 parts of perilla leaf. The Chinese medicinal composition provided by the invention can be prepared into clinically suitable oral preparations according to traditional Chinese medicine conventional preparation methods. The Chinese medicinal composition significantly shortens the immobility time of mouse tail suspension and forced swimming, significantly increases the hand shaking time of mice given 5-hydroxytryptophan, has a significant antagonistic effect on mouse blepharoptosis and hypothermia caused by reserpine, and significantly antagonizes mouse body temperature decline caused by high dose apomorphine. According to the invention, research results show that the Chinese medicinal composition has a significant anti-mouse experimental depression effect.

Description

Chinese medicine composition and preparation method thereof and application

Technical field

The present invention relates to pharmaceutical composition, particularly Chinese medicine composition and preparation method thereof and application, belong to the field of Chinese medicines.

Background technology

Depression (depression) is a kind of common mental sickness, has the features such as high prevalence, high relapse rate, high disability rate and high fatality rate, is called as " the first psychological killer ".Be embodied in: lasting depressive emotion, and this emotion for a long time obviously exceedes necessary limit, and self-doubt, the obvious reduction of feeling body energy cannot be realized happy in any interesting activity.This class obstacle also can cause patient's somatic function imbalance, as sleep disordered, local body pain or loss of appetite etc.In western countries, depression is a kind of prevailing mental sickness, wherein the sickness rate of lifelong depression between 6%～8%, along with the progressively aging of population, depression more than 60 years old the sickness rate in crowd up to 20%～50%.In patient, have 65%～80% to there will be serious suicide idea, 45%～55% there will be suicide or autolesionism, and lifelong Mortality of suicide is up to 15%～25%." World Health Report in 2002 " delivered according to World Health Organization (WHO), depression has become the fourth-largest illness in the world, may become and be only second to cardiopathic second largest disease to the year two thousand twenty depression.Investigation in 2012 shows, in China, the public's Psychological Health Problem allows of no optimist, and exceed 2,600 ten thousand people and suffer from depression in various degree, but that 90% patient does not recognize is ill.

About the definite cause of disease of depression, completely unclear yet so far, may there is the factor of biological and psychological two aspects.Biological factor mainly comprises the neural biochemical metabolism disorder of heredity and body; Psychological factor comprises home influence, social influence and stressors etc.

The synthetic drug of Cure of depression mainly comprises: 1, serotonin reuptake inhibitor (SSRI) class medicine, as paroxetine, Sertraline, fluoxetine, citalopram, fluvoxamine etc., that its common untoward reaction has is nauseating, vomiting, anorexia, constipation, diarrhoea, xerostomia, tremble, insomnia, anxiety and sexual dysfunction etc.2, NRI (SNRI) class medicine, as venlafaxine, duloxetine, that its common adverse reactions has is nauseating, xerostomia, perspiration, weak, anxiety, tremble, sexual impotence and defective ejaculation, when heavy dose of, some patients were blood pressure may slightly raise; 3, NE can and specificity 5-HT can antidepressants (NaSSAs class antidepressants), as mirtazapine, its common adverse reactions be calmness, drowsiness, dizziness, tired, appetite and body weight increase; 4, amfebutamone, the weak inhibitor of norepinephrine, 5-hydroxy tryptamine, dopamine reuptake, its common untoward reaction has: excitement, xerostomia, insomnia, headache or migraine, feel sick, vomiting, constipation, tremble, hyperhidrosis.5, for some anxieties obviously, with the patient of sleep disorder, can short-term use some Benzodiazepineses (stable class) medicine or sleeping medicine that some are novel, as zolpidem, zopiclone.For some serious symptoms, even, with the patient of psychotic symptoms, can merge antipsychotic medications.

Existing synthetic antidepressants, as paroxetine, Sertraline, fluoxetine, citalopram, fluvoxamine, venlafaxine, duloxetine, amfebutamone etc., for clinical treatment depression, often untoward reaction is more, under therapeutic dose, often have nauseating, vomiting, anorexia, constipation, diarrhoea, xerostomia, tremble, insomnia, anxiety and sexual dysfunction etc., the matters of aggravation that indivedual patients also there will be suicidal tendency to increase the weight of, patient treatment poor compliance, often quite a few patient can not obtain satisfied curative effect after treatment.

Since ancient times, China's Chinese medicine is accumulating rich experience aspect control depression, compound of Chinese medicine depression has can long-term taking and can not cause the advantages such as multiple untoward reaction, patient treatment compliance that synthetic drug causes be good, but antidepressant Chinese patent medicine is in the market few, still lack the Chinese patent medicine of the little Cure of depression of untoward reaction evident in efficacy.

BANXIA HOUPU TANG: the party's using pungent drugs for dispersion and bitter drugs for purgation of working hard, come from " Medical Treasures of the Golden Chamber ", composed as follows: the Rhizoma Pinelliae (12g), Cortex Magnoliae Officinalis (9g), Poria (12g), Rhizoma Zingiberis Recens (9g), Folium Perillae (6g).The party is with promoting QI to circulate and dispersing the agglomeration of the pathogens, and lowering the adverse-rising QI and eliminating phlegm is main, cures mainly the globus hystericus of stagnation of Qi and phelgm.The party demonstrate,proves many because mental disorder pent-up is due to the throat.Feelings will is unsuccessful, depression of liver-QI, and lung stomach loses a surname Yu and falls, and body fluid is cloth not, poly-and be expectorant, and mental disorder is fought mutually, ties in throat, therefore see in pharynx if any thing resistance, cough up and can not tell, can not swallow any more; Lung stomach loses a surname Yu and falls, and also can cause functional activity of QI being not smooth in the heart, and sees that fullness in the chest and hypochondrium is vexed or cough dyspnea with rapid respiration or nausea and vomiting etc.Not all right strongly fragrant not the understanding of gas, expectorant is not changed knot difficulty and is fallen apart, therefore the method for suitable promoting QI to circulate and dispersing the agglomeration of the pathogens, eliminating phlegm and lowering adverse QI.The pungent temperature people's lung of Rhizoma Pinelliae stomach in side, dissipating phlegm and resolving masses, normalizing the stomach by guiding QI downward, is monarch drug.Cortex Magnoliae Officinalis toil is warm in nature, and the therapeutic method to keep the adverse QI flowing downwards, except full, helps Rhizoma Pinelliae eliminating stagnation sending down the abnormal ascending QI, is ministerial drug.The sweet light eliminating dampness by diuresis spleen invigorating of Poria, to help the Rhizoma Pinelliae to reduce phlegm; The pungent temperature eliminating stagnation of Rhizoma Zingiberis Recens, stomach function regulating preventing or arresting vomiting, and the poison of Rhizoma Pinelliae Preparata; The circulation of qi promoting of Folium Perillae fragrance, reason lung soothing liver-QI, helps Cortex Magnoliae Officinalis circulation of qi promoting the chest stuffiness relieving, a surname to lead to the gas of pent-up, is adjuvant drug altogether.Full side is arduous to be share, pungent with promoting QI to circulate and dispersing the agglomeration of the pathogens, bitter with dampness sending down the abnormal ascending QI, makes to be strongly fragrantly so incensed that thin, and sputum must be changed, and the globus hystericus of mental disorder pent-up is from removing.The party is usually used in hysteria, stomach neurosis, chronic pharyngitis, chronic bronchitis, esohagismus etc. and belongs to stagnation of QI causing phlegm retention person.The demonstration of modern Chinese medicine pharmaceutical research, the party has antidepressant effect.

Sini San: come from treatise on Febrile Diseases, composed as follows: Radix Bupleuri (6 grams), Fructus Aurantii Immaturus (6 grams), Radix Paeoniae (6 grams), Radix Glycyrrhizae Preparata (6 grams).Cold limbs person, be hands and feet being not warm also.In its card is transmitted scriptures because of exopathogen, mechanism of qi is strongly fragrant holding back for it, must not catharsis, cause stagnation of YANG QI in the interior, and can not reach in four ends, and see hands and feet being not warm.This kind " cold limbs " fainted against there being essential distinction with the extremity of the positive excess of YIN that declines.As Li Zhongzi's cloud: " though this card cloud cold limbs, must be very not cold, or finger tepor, or arteries and veins is heavy micro-, is the card of containing sun in the moon, only gas is not declared logically, is to be cold of the limbs.Therefore " control suitable expelling pathogenic factors from the exterior resolving depression, harmonizing the functional activities of vital QI is method.In side, get Radix Bupleuri and enter liver and gall warp, rise and send out yang-energy, dispersing the stagnated live-QI to relieve the stagnation of QI, expelling pathogenic factors from the exterior is gone out, and is monarch drug.Radix Paeoniae Alba preserving YIN with astringents and nourishing blood easing the affected liver is minister, share with Radix Bupleuri, and to take a tonic or nourishing food to build up one's health liver blood, bar reaches irritability, can make Radix Bupleuri rise the fraud of loose and lossless impairment of YIN blood.Assistant is with the Fructus Aurantii Immaturus resolving depression of regulating the flow of vital energy, and the knot that expels the heat-evil brokenly, associates with Radix Bupleuri, and the merit of happy mechanism of qi is strengthened in one rise and one drop, and plays the effect of ascending the clear and descending the turbid; Match with the Radix Paeoniae Alba, can regulate the flow of vital energy again and blood, QI and blood is in harmonious proportion.Make with Radix Glycyrrhizae, coordinating the actions of various ingredients in a prescription, strengthening the spleen and in.Comprehensive four medicines, play expelling pathogenic factors from the exterior resolving depression altogether, and the effect of smooth the liver and regulate the spleen makes heresy go strongly fragrant solution, and QI and blood is adjusted smooth, clear positive must stretching, cold limbs spontaneous recovery.The party has and removes stagnant heat, depressed liver-energy dispersing and QI regulating, and for cold limbs due to intense heat in the interior hands and feet being not warm, breast side of body feeling of fullness, dysentery lumbago, and stomachache, stomachache due to incoordination between the liver and stomach.The demonstration of modern Chinese medicine pharmaceutical research, the party has antidepressant effect.

Fructus Gardeniae: come from " Bencao Tujing ", there is pathogenic fire purging relieving restlessness, clearing away heat-damp and promoting diuresis, effect of removing pathogenic heat from blood and toxic substance from the body.Cure mainly that calentura is vexed, liver-fire conjunctival congestion, headache, damp and hot yellow infantile malnutrition, stranguria, dysentery hematuria, aphtha of the mouth and tongue, sore swollen toxin, sprain and swell and ache.Modern Chinese medicine pharmaceutical research shows, Fructus Gardeniae have hepatic cholagogic, gastric acid secretion inhibiting, promotion pancreatic secretion, tranquilizing soporific, reduce blood pressure, the effect such as analgesia.

Summary of the invention

Goal of the invention

The invention provides Chinese medicine composition and preparation method thereof and application, this Chinese medicine composition is made up of Poria, the Rhizoma Pinelliae, Radix Bupleuri, Fructus Aurantii Immaturus, the Radix Paeoniae Alba, Cortex Magnoliae Officinalis, Radix Glycyrrhizae, Fructus Gardeniae and Folium Perillae.

Technical scheme

Chinese medicine composition of the present invention is made up of 9 taste Chinese medicines, is respectively Poria, the Rhizoma Pinelliae, Radix Bupleuri, Fructus Aurantii Immaturus, the Radix Paeoniae Alba, Cortex Magnoliae Officinalis, Radix Glycyrrhizae, Fructus Gardeniae and Folium Perillae.

Chinese medicine composition provided by the invention, made by the crude drug of following weight portion:

Poria 10-40 part, Rhizoma Pinelliae 10-40 part, Radix Bupleuri 10-40 part, Fructus Aurantii Immaturus 10-40 part, Radix Paeoniae Alba 10-40 part, Cortex Magnoliae Officinalis 10-40 part, Radix Glycyrrhizae 6-24 part, Fructus Gardeniae 10-40 part, Folium Perillae 10-40 part.

Most preferred Chinese medicine composition, the weight portion of each crude drug is: 20 parts, Poria, 20 parts of the Rhizoma Pinelliaes, 20 parts of Radix Bupleuri, 20 parts of Fructus Aurantii Immaturuss, 20 parts of the Radix Paeoniae Albas, 20 parts of Cortex Magnoliae Officinalis, 12 parts, Radix Glycyrrhizae, 20 parts of Fructus Gardeniaes, 20 parts of Folium Perillae.

The extract of Chinese medicine composition of the present invention can add various adjuvants required while preparing different dosage form, such as disintegrating agent, lubricant, adhesive etc., be prepared into any conventional oral formulations with conventional method of Chinese medicinal, can be for example tablet, capsule, granule, soft capsule, pill, drop pill or oral liquid etc., be preferably granule.

Preferably, a kind of method of preparing above-mentioned Chinese medicine composition, comprises following content:

(1) take each raw material by following weight parts: Poria 10-40 part, Rhizoma Pinelliae 10-40 part, Radix Bupleuri 10-40 part, Fructus Aurantii Immaturus 10-40 part, Radix Paeoniae Alba 10-40 part, Cortex Magnoliae Officinalis 10-40 part, Radix Glycyrrhizae 6-24 part, Fructus Gardeniae 10-40 part, Folium Perillae 10-40 part;

(2) medicine mixes and then uses slow fire boiling 0.5-1 hour with 6-10 times of weight water soaking 2 hours afterwards, filters, and adds for the second time 6-10 times of weight water, and slow fire boiling 0.5-1 hour, filters, and merging filtrate is concentrated, for subsequent use;

(3) by concentrated solution drying under reduced pressure prepared step (2), obtain dry extract;

(4) dry extract is pulverized, and crosses 80 mesh sieves, adds appropriate dextrin, fully mix, and granulation, dry, to obtain final product.

After medicine being mixed in step (2), with 8 times of weight water soakings 2 hours, then use slow fire boiling 0.5 hour, filter, add for the second time 8 times of weight water, slow fire boiling 0.5 hour, filters, merging filtrate, when filtrate is concentrated into 60 DEG C of mensuration, relative density is 1.10-1.12, obtains concentrated solution.

Drying under reduced pressure described in step (3) refers to that at pressure be 0.06-0.08Mpa, and temperature is drying under reduced pressure under the condition of 80 DEG C.

Any one above-mentioned Chinese medicine composition is in the application of preparing in Cure of depression medicine.

The usage of medicine of the present invention and consumption: the dosage of medicine of the present invention depends on concrete dosage form, and the factor such as age of patient, body weight, health status.As guidance: granule, oral, in 1 generation (12g crude drug/bag), 2 times on the one 6 weeks is a course for the treatment of.

Beneficial effect

1, the present invention reconfigures and deducts Rhizoma Zingiberis Recens, increases after Fructus Gardeniae BANXIA HOUPU TANG and Sini San, forms new Chinese medicine composition, and its composition comprises: Poria, the Rhizoma Pinelliae, Radix Bupleuri, Fructus Aurantii Immaturus, the Radix Paeoniae Alba, Cortex Magnoliae Officinalis, Radix Glycyrrhizae, Fructus Gardeniae and Folium Perillae.The pungent temperature people's lung of Rhizoma Pinelliae stomach in side, dissipating phlegm and resolving masses, normalizing the stomach by guiding QI downward; Cortex Magnoliae Officinalis toil is warm in nature, and the therapeutic method to keep the adverse QI flowing downwards is except full; The sweet light eliminating dampness by diuresis spleen invigorating of Poria; The circulation of qi promoting of Folium Perillae fragrance, reason lung soothing liver-QI; Radix Bupleuri enters liver and gall warp, and rise and send out yang-energy, dispersing the stagnated live-QI to relieve the stagnation of QI, expelling pathogenic factors from the exterior is gone out; Radix Paeoniae Alba preserving YIN with astringents and nourishing blood easing the affected liver, takes a tonic or nourishing food to build up one's health liver blood, and bar reaches irritability; The Fructus Aurantii Immaturus resolving depression of regulating the flow of vital energy, the knot that expels the heat-evil brokenly, happy mechanism of qi, ascending the clear and descending the turbid; The Radix Paeoniae Alba is regulated the flow of vital energy and blood, and QI and blood is in harmonious proportion; Radix Glycyrrhizae, coordinating the actions of various ingredients in a prescription, strengthening the spleen and in; Fructus Gardeniae pathogenic fire purging relieving restlessness, clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body.The all medicine compatibility application of said composition, have promoting QI to circulate and dispersing the agglomeration of the pathogens, lowering the adverse-rising QI and eliminating phlegm, and depressed liver-energy dispersing and QI regulating, expelling pathogenic factors from the exterior resolving depression, makes heresy go effect of strongly fragrant solution.Whether extract prepared by said composition has antidepressant effect, has no report.The present invention finds that this Chinese medicine composition has significant antidepressant activity, and its activity is better than BANXIA HOUPU TANG and Sini San, can be in the situation that significantly reducing dosage, obtain better antidepressant curative effect.

2, the antidepressant effect of Chinese medicine composition of the present invention is mainly reflected in: Chinese medicine composition of the present invention significantly shortens mouse tail suspension and forced swimming dead time; Significantly increase a number of times that gets rid of that gives mice after 5-HTP; Significantly antagonism reserpine induced mice blepharoptosis and body temperature reduce; The mouse temperature that significantly antagonism high dose apomorphine causes declines.Chinese medicine composition of the present invention has the depressed effect of significant anti-mouse experiment.

Detailed description of the invention

Further describe by the following examples the present invention, it should be understood that these embodiment, only for the object of illustration, never limit the scope of the invention.

The preparation of embodiment 1 granule

Take each raw material by following weight: Poria 200g, Rhizoma Pinelliae 200g, Radix Bupleuri 200g, Fructus Aurantii Immaturus 200g, Radix Paeoniae Alba 200g, Cortex Magnoliae Officinalis 200g, Radix Glycyrrhizae 120g, Fructus Gardeniae 200g, Folium Perillae 200g;

After said medicine is mixed, with 8 times of weight water soakings 2 hours, then use slow fire boiling 0.5 hour, filter, add for the second time 8 times of weight water, slow fire boiling 0.5 hour, filters, merging filtrate, when filtrate is concentrated into 60 DEG C of mensuration, relative density is 1.10-1.12, obtains concentrated solution; Be 0.06-0.08Mpa by the concentrated solution of preparation at pressure, temperature is drying under reduced pressure under the condition of 80 DEG C, obtains dry extract; Dry extract is pulverized, and crosses 80 mesh sieves, adds appropriate dextrin, fully mix, and granulation, dry, to obtain final product.

The preparation of embodiment 2 tablets

Take each raw material by following weight: Poria 200g, Rhizoma Pinelliae 200g, Radix Bupleuri 200g, Fructus Aurantii Immaturus 200g, Radix Paeoniae Alba 200g, Cortex Magnoliae Officinalis 200g, Radix Glycyrrhizae 120g, Fructus Gardeniae 200g, Folium Perillae 200g;

After said medicine is mixed, with 8 times of weight water soakings 2 hours, then use slow fire boiling 0.5 hour, filter, add for the second time 8 times of weight water, slow fire boiling 0.5 hour, filters, merging filtrate, when filtrate is concentrated into 60 DEG C of mensuration, relative density is 1.10-1.12, obtains concentrated solution; Be 0.06-0.08Mpa by the concentrated solution of preparation at pressure, temperature is drying under reduced pressure under the condition of 80 DEG C, obtains dry extract; Dry extract is pulverized, and crosses 80 mesh sieves, adds appropriate dextrin, granulates, dry, granulate, tabletting make tablet.

The preparation of embodiment 3 capsules

Take each raw material by following weight: Poria 200g, Rhizoma Pinelliae 200g, Radix Bupleuri 200g, Fructus Aurantii Immaturus 200g, Radix Paeoniae Alba 200g, Cortex Magnoliae Officinalis 200g, Radix Glycyrrhizae 120g, Fructus Gardeniae 200g, Folium Perillae 200g;

After said medicine is mixed, with 8 times of weight water soakings 2 hours, then use slow fire boiling 0.5 hour, filter, add for the second time 8 times of weight water, slow fire boiling 0.5 hour, filters, merging filtrate, when filtrate is concentrated into 60 DEG C of mensuration, relative density is 1.10-1.12, obtains concentrated solution; Be 0.06-0.08Mpa by the concentrated solution of preparation at pressure, temperature is drying under reduced pressure under the condition of 80 DEG C, obtains dry extract; Dry extract is pulverized, and crosses 80 mesh sieves, adds appropriate dextrin, granulates, dry, granulate, incapsulates and make capsule.

The impact test of embodiment 4 Chinese medicine composition of the present invention on the desperate behavior of mice

1 experiment material

1.1 laboratory animal

Clean level male mice, body weight (20 ± 2g).Laboratory animal is provided by Yangzhou University's animal reproduction center, laboratory animal production licence: SCXK (Soviet Union) 2007.0001.The free diet of whole experimentation animal and drinking-water.25 DEG C of room temperatures, environment peace and quiet.

1.2 Experimental agents, reagent and instrument

1.2.1 Experimental agents

Be subject to reagent: Chinese medicine composition extract dry powder of the present invention.

Sini San: Haiwang Jinxiang Chinese Medicine Pharmaceutical Co., Ltd., Fuzhou City.

BANXIA HOUPU TANG: take the Rhizoma Pinelliae (180g), Cortex Magnoliae Officinalis (150g), Poria (150g), Rhizoma Zingiberis Recens (120g), Folium Perillae (under rear, 90g).After said medicine is mixed, with 8 times of weight water soakings 2 hours, then use slow fire boiling 0.5 hour, filter, add for the second time 8 times of weight water, slow fire boiling 0.5 hour, filters merging filtrate, concentrated, be 0.06-0.08Mpa by the concentrated solution of preparation at pressure, temperature is drying under reduced pressure under the condition of 80 DEG C, obtains dry extract.

Fluoxetine Hydrochloride: Pharmaceutical Co Ltd, Changzhou Pharmaceutical Factory No.4 produces.

Before administration, will be subject to reagent, Sini San, BANXIA HOUPU TANG and fluoxetine 0.5%CMC-Na to grind to form suspension, and be placed in 4 DEG C of refrigerators and save backup.

1.2.2 reagent and instrument

Reagent: 5-HTP: Hangzhou Zhong Xiang Chemical Co., Ltd. produces, lot number: CAC071205.Reserpine: Changzhou Pharmaceutical Factory Co., Ltd produces, lot number: 10070911.Apomorphine: be sigma company product.

Instrument: stopwatch, 2500mL beaker, outstanding tail support, electronic clinical thermometer (model MC-106B, Omron (China) Co., Ltd.), electronic balance (Beijing Sai Duolisi balance company limited), gavage pin, opens wild experimental box (diameter 30cm, high 20cm).

2 experimental techniques

2.1 outstanding tail experiments

Choose 50 male mices, body weight 18-22g, by body weight, mice is divided into 5 groups at random, be respectively: blank group, fluoxetine group (0.01g/kg), is subject to reagent group (by crude drug amount 2g/kg), BANXIA HOUPU TANG group (pressing crude drug amount 5g/kg), Sini San group (pressing crude drug amount 5g/kg), 10 every group.Each administration group and the administration of fluoxetine group mouse stomach, administration volume is 0.2mL/10g body weight, blank group mouse stomach equal-volume normal saline.Each group mice successive administration 7 days, after last administration 1 hour, mice is inverted, afterbody uses rubberized fabric adhere on outstanding tail apparatus apart from sharp 1cm place, head, apart from the about 20cm of desktop, makes mice all around all without climbing the place of grabbing, and between every two mices, separates animal sight line with baffle plate, to prevent mutual interference, observe in every animal 6 minutes the accumulative total dead time (stationarity indices is that mice is all motionless except breathing extremity) of latter 4 minutes.

2.2 forced swimming experiments

Choose 50 male mices, body weight 18-22g, by body weight, mice is divided into 5 groups at random, be respectively: blank group, fluoxetine group (0.01g/kg), is subject to reagent group (by crude drug amount 2g/kg), BANXIA HOUPU TANG group (pressing crude drug amount 5g/kg), Sini San group (pressing crude drug amount 5g/kg), 10 every group.Each administration group and the administration of fluoxetine group mouse stomach, administration volume is 0.2mL/10g body weight, blank group mouse stomach equal-volume normal saline.Each group mice successive administration 7 days, after last administration, puts into respectively 2500mL large beaker by each group of mice, the high 25cm of walls of beaker, diameter 15cm, depth of water 10cm, 25 DEG C of water temperatures, separate animal sight line with baffle plate between every two mices, to prevent mutual interference, while just putting into beaker, mice swimming is struggled, after a period of time, because despair activity weakens, show as the micro-song of health, surface in nostril, be floating state, extremity are motionless.The accumulative total dead time of latter 4 minutes in every animal of observed and recorded 6 minutes (stationarity indices is that mice is all motionless except breathing extremity).

2.3 open wild experiment

Choose 50 male mices, body weight 18-22g, by body weight, mice is divided into 5 groups at random, be respectively: blank group, fluoxetine group (0.01g/kg), is subject to reagent group (by crude drug amount 2g/kg), BANXIA HOUPU TANG group (pressing crude drug amount 5g/kg), Sini San group (pressing crude drug amount 5g/kg), 10 every group.Each administration group and the administration of fluoxetine group mouse stomach, administration volume is 0.2mL/10g body weight, blank group mouse stomach equal-volume normal saline.Experimental provision is diameter 30cm, the cylinder of high 20cm, quietly under environment, carrying out this laboratory observation, observation index: taking animal pass through bottom surface grid number (track route and grid cross point number) as horizontal anomalous movement point, upright number of times (the liftoff 1cm of two forelimbs above or seek connections with the number of times of wall) is vertical activity score, and measures the number of times that washes one's face.Mice centering grid endoadaptation, after 2 minutes, is recorded to spontaneous activity in mice in 4 minutes, and every zoometry once.

Due to 2.45-hyroxytrypophan, get rid of head experiment

Choose 50 male mices, body weight 18-22g, by body weight, mice is divided into 5 groups at random, be respectively: blank group, fluoxetine group (0.01g/kg), is subject to reagent group (by crude drug amount 2g/kg), BANXIA HOUPU TANG group (pressing crude drug amount 5g/kg), Sini San group (pressing crude drug amount 5g/kg), 10 every group.Each administration group and the administration of fluoxetine group mouse stomach, administration volume is 0.2mL/10g body weight, blank group mouse stomach equal-volume normal saline.Each group mice successive administration 7 days, after last administration, 30 minutes each group mouse peritoneal injection 5-hydroxyryptophan 5mg/kg, are placed in cage single mice subsequently, start to observe after 10 minutes, record mice in 10 minutes and get rid of a number of times.

2.5 reserpine antagonistic experiments

Choose 50 male mices, body weight 18-22g, by body weight, mice is divided into 5 groups at random, be respectively: blank group, fluoxetine group (0.01g/kg), is subject to reagent group (by crude drug amount 2g/kg), BANXIA HOUPU TANG group (pressing crude drug amount 5g/kg), Sini San group (pressing crude drug amount 5g/kg), 10 every group.Each administration group and the administration of fluoxetine group mouse stomach, administration volume is 0.2mL/10g body weight, blank group mouse stomach equal-volume normal saline.Each group mice successive administration 7 days, at once subcutaneous injection of reserpine 5mg/kg after last administration, after 1 hour, observe respectively: (1) respectively organizes the blepharoptosis degree of mice, and add up in the mode of score, marking standard is: widening the view is completely 0 point, and 1/4 closes one's eyes is 1 point, and 2/4 closes one's eyes is 2 points, 3/4 closes one's eyes is 3 points, and closing one's eyes is completely 4 points.(2) clinical thermometer is inserted to animal anal 1cm place and measure anus temperature.

The temperature decline experiment that 2.6 high dose apomorphine cause

Choose 50 male mices, body weight 18-22g, by body weight, mice is divided into 5 groups at random, be respectively: blank group, fluoxetine group (0.01g/kg), is subject to reagent group (by crude drug amount 2g/kg), BANXIA HOUPU TANG group (pressing crude drug amount 5g/kg), Sini San group (pressing crude drug amount 5g/kg), 10 every group.Each administration group and the administration of fluoxetine group mouse stomach, administration volume is 0.2mL/10g body weight, blank group mouse stomach equal-volume normal saline.Mice last administration pre-test anus temperature, administration is subcutaneous injection apomorphine (16mg/kg) after 90 minutes, again measures anus temperature after 30 minutes, calculates administration front and back mice anus temperature and changes difference.2.7 statistical procedures

All data all represent with mean ± standard deviation (Mean ± SD), adopt one-way analysis of variance (ANOVA) to carry out statistical analysis to experimental data, P < 0.05 is for having significant difference, and P < 0.01 is for having utmost point significant difference.

3 experimental results

3.1 are subject to the impact of reagent on the desperate model of mouse tail suspension

Experimental result is in table 1, compared with blank group, be subject to reagent under the dosage of 2g/kg, extremely significantly to shorten the mouse tail suspension dead time (P < 0.01), effect is better than Sini San (P < 0.05) and BANXIA HOUPU TANG (P < 0.05) shortening to the mouse tail suspension dead time under the dosage of 5g/kg under the dosage of 5g/kg.

Table 1 is subject to the impact (Mean ± SD) of reagent on the mouse tail suspension dead time

Note: with blank group ratio, * P < 0.05, * * P < 0.01

3.2 are subject to the impact of reagent on the desperate model of mice forced swimming

Experimental result is in table 2, compared with blank group, be subject to reagent under the dosage of 2g/kg, extremely significantly to shorten the mice forced swimming dead time (P < 0.01), effect is better than Sini San (P < 0.05) and BANXIA HOUPU TANG (P < 0.05) shortening to the mice forced swimming dead time under the dosage of 5g/kg under the dosage of 5g/kg.

Table 2 is subject to the impact (Mean ± SD) of reagent on the mice forced swimming dead time

Note: with blank group ratio, * P < 0.05, * * P < 0.01

3.3 are subject to the impact of reagent on spontaneous activity in mice

Experimental result, in table 3, opening in wild experiment, is subject to reagent group, Sini San group and BANXIA HOUPU TANG group mice autonomic activities there is no significant difference compared with blank group.

Table 3 is subject to the impact (Mean ± SD) of reagent on spontaneous activity in mice

Note: with blank group ratio, * P < 0.05, * * P < 0.01

3.4 are subject to reagent the mice of five hydroxytryptamine acid induction to be got rid of to the impact of a behavior

Experimental result is in table 4, compared with blank group, the mice that is subject to reagent extremely significantly to increase 5-HTP induction under the dosage of 2g/kg gets rid of a number of times (P < 0.01), and effect is better than Sini San (P < 0.05) and BANXIA HOUPU TANG increase that (P < 0.05) gets rid of a number of times to the mice of 5-HTP induction under the dosage of 5g/kg under the dosage of 5g/kg.

Table 4 is subject to reagent the mice of 5-HTP induction to be got rid of to the impact (Mean ± SD) of a behavior

Note: with blank group ratio, * P < 0.05, * * P < 0.01

3.5 are subject to the impact of reagent on reserpine antagonistic experiment

3.5.1 be subject to reagent reserpine to be caused to the impact of mice blepharoptosis

Experimental result is in table 5, compared with blank group, be subject to the reagent mice blepharoptosis (P < 0.05) that significantly antagonism reserpine causes under the dosage of 2g/kg, effect is better than Sini San (P < 0.05) and BANXIA HOUPU TANG (P < 0.05) antagonism to reserpine induced mice blepharoptosis under the dosage of 5g/kg under the dosage of 5g/kg.

Table 5 is subject to reagent reserpine to be caused to the impact (Mean ± SD) of mice blepharoptosis

Note: with blank group ratio, * P < 0.05, * * P < 0.01

3.5.2 be subject to reagent to cause on reserpine the impact that mouse temperature declines

Experimental result is in table 6, injection reserpine is after 2 hours, compared with blank group, be subject to the reagent temperature decline (P < 0.01) that extremely significantly antagonism reserpine causes under the dosage of 2g/kg, effect is better than Sini San (P < 0.05) and BANXIA HOUPU TANG (P < 0.05) antagonism to reserpine induced mice temperature decline under the dosage of 5g/kg under the dosage of 5g/kg.

Table 6 is subject to reagent to cause on reserpine the impact (Mean ± SD) that mouse temperature declines

Note: with blank group ratio, * P < 0.05, * * P < 0.01

3.6 impacts that declined by mouse temperature that reagent causes high dose apomorphine

Experimental result is in table 7, compared with blank group, declined (P < 0.01) by the reagent mouse temperature that extremely significantly antagonism apomorphine causes under the dosage of 2g/kg, effect is better than Sini San (P < 0.05) and BANXIA HOUPU TANG (P < 0.05) antagonism to apomorphine induced mice temperature decline under the dosage of 5g/kg under the dosage of 5g/kg.

The impact (Mean ± SD) that table 7 is declined by mouse temperature that reagent causes high dose apomorphine

Note: with blank group ratio, * P < 0.05, * * P < 0.01.

Claims (8)

1. Chinese medicine composition, made by the crude drug of following weight portion:

Poria 10-40 part, Rhizoma Pinelliae 10-40 part, Radix Bupleuri 10-40 part, Fructus Aurantii Immaturus 10-40 part, Radix Paeoniae Alba 10-40 part, Cortex Magnoliae Officinalis 10-40 part, Radix Glycyrrhizae 6-24 part, Fructus Gardeniae 10-40 part, Folium Perillae 10-40 part.

2. according to the Chinese medicine composition of claim 1, it is characterized in that, the weight portion of each crude drug is: 20 parts, Poria, 20 parts of the Rhizoma Pinelliaes, 20 parts of Radix Bupleuri, 20 parts of Fructus Aurantii Immaturuss, 20 parts of the Radix Paeoniae Albas, 20 parts of Cortex Magnoliae Officinalis, 12 parts, Radix Glycyrrhizae, 20 parts of Fructus Gardeniaes, 20 parts of Folium Perillae.

3. according to the Chinese medicine composition of claim 1 or 2, it is characterized in that, be prepared into oral formulations according to Chinese medicine conventional formulation method.

4. according to the Chinese medicine composition of claim 3, it is characterized in that, described oral formulations is tablet, capsule, soft capsule, granule, pill, drop pill or oral liquid.

5. a method of preparing claim 1 Chinese medicine composition, comprising:

(1) take each raw material by following weight parts: Poria 10-40 part, Rhizoma Pinelliae 10-40 part, Radix Bupleuri 10-40 part, Fructus Aurantii Immaturus 10-40 part, Radix Paeoniae Alba 10-40 part, Cortex Magnoliae Officinalis 10-40 part, Radix Glycyrrhizae 6-24 part, Fructus Gardeniae 10-40 part, Folium Perillae 10-40 part;

(2) medicine mixes and then uses slow fire boiling 0.5-1 hour with 6-10 times of weight water soaking 2 hours afterwards, filters, and adds for the second time 6-10 times of weight water, and slow fire boiling 0.5-1 hour, filters, and merging filtrate is concentrated, for subsequent use;

(3) by concentrated solution drying under reduced pressure prepared step (2), obtain dry extract;

(4) dry extract is pulverized, and crosses 80 mesh sieves, adds appropriate dextrin, fully mix, and granulation, dry, to obtain final product.

6. according to the method for claim 5, it is characterized in that, after in step (2), medicine being mixed, with 8 times of weight water soakings 2 hours, then use slow fire boiling 0.5 hour, filter, add for the second time 8 times of weight water, slow fire boiling 0.5 hour, filter, merging filtrate, when filtrate is concentrated into 60 DEG C of mensuration, relative density is 1.10-1.12, obtains concentrated solution.

7. according to the method for claim 5, it is characterized in that, the drying under reduced pressure described in step (3) refers to that at pressure be 0.06-0.08Mpa, and temperature is drying under reduced pressure under the condition of 80 DEG C.

8. any one Chinese medicine composition of claim 1-4 is in the application of preparing in Cure of depression medicine.