CN104073433A - Large quantitatively counting microscope detection glass slide for microorganisms - Google Patents
Large quantitatively counting microscope detection glass slide for microorganisms Download PDFInfo
- Publication number
- CN104073433A CN104073433A CN201410313573.XA CN201410313573A CN104073433A CN 104073433 A CN104073433 A CN 104073433A CN 201410313573 A CN201410313573 A CN 201410313573A CN 104073433 A CN104073433 A CN 104073433A
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- sample liquid
- counting
- sample solution
- detection
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Optics & Photonics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a large quantitatively counting microscope detection glass slide for microorganisms. According to the technical scheme, a mark plate is arranged on one side of a glass slide plane, 2-4 slender sample solution pools which are 0.5-3mm in width and 10-100microlitters in volume and provided with counting scales at the bottoms are arranged on the other side of a glass slide plane, a circular sample solution injection opening is formed in the end head of each sample solution pool, has a diameter which is greater than the width of the sample solution pool, and penetrating through the sample solution pool, and the widths and the volumes of all the sample solution pool are different in a limit range. Because the sample solution pools are slender and small in diameter, the sample solution pools can be covered simultaneously by the observation view of a microscope, and the observation view gradually moves from one end of a detection pool to the other end along the counting scales, thus avoiding the phenomenon of missing detection or repeated detection, and a counting detection error caused by the movements of the microorganisms; therefore, the large quantitatively counting microscope detection glass slide for the microorganisms is high in operability and has outstanding substantive features and remarkable improvements in comparison with the existing glass slides.
Description
Technical field
The present invention relates to a kind of microorganism count and detect slide glass, specifically a kind of large-scale microorganism quantitative counting microscopic inspection slide glass, belongs to biometric detection field.
Background technology
In agronomy, forestry, medical science and microbiology field, microscopic examination, Density Detection and the counting of microorganism is one of conventional technique means and requisite operation link, as the Density Detection of pathogenic micro-organism etc., and slide glass is the important assistive device detecting.The slide glass of observing and counting for microorganism at present has broad variety, most typical is blood counting chamber, but this device is applicable to the counting of small-sized microorganism, as the detection of bacterium and yeast etc., and be not suitable for microalgae, plankton, the relatively large microorganism of nematode equal-volume to detect and count.In published patent documentation, relate to the having of detection by quantitative " the special detection by quantitative slide of humoral specimen " (CN202757874U) with " a kind of quantitative glass carrier " (CN201583723U), but being the automatic samples in order conveniently to carry out humoral specimen, these two kinds of slide glasss detect and minimizing waste liquid operational pollution, and realize the quantification application of sample of liquid sample, or overflow slide glass and pollute Stage microscope for fear of unnecessary tracer liquid sample, do not relate to the microorganism in sample is carried out to accurate metering.In addition; the sample liquid pool area of these two kinds of slide glasss is excessive; under microscope, its width is far longer than microscopical field range; therefore in detection, usually there will be the phenomenon of omitting detection or duplicate detection; therefore can not count accurately microorganism wherein, all the more so when especially detected object is movable microorganism.Therefore, still lack a kind of slide glass of desirable applicable relatively large microorganism (as nematode) counting at present.
Summary of the invention
The object of the invention is to overcome existing microorganism count slide glass and be unsuitable for large-scale microorganism accurate metering, sample liquid pool area is excessive, far beyond field of microscope scope, easily cause the drawback of omitting detection or duplicate detection and cause counting error, and a kind of large-scale microorganism quantitative counting microscopic inspection slide glass is disclosed, to make up the deficiencies in the prior art.
Technical scheme of the present invention is as follows:
On year glass body plane, a side is provided with marking plate, opposite side is provided with the sample liquid pond of 2-4 elongated shape, its width is 0.5-3mm, capacity is 10-100ul, counting scale is carved with in bottom, the termination in each sample liquid pond is provided with circular sample liquid inlet, and diameter is greater than the width in sample liquid pond and connects with sample liquid pond.
The sample liquid pond of setting of the present invention is that elongated shape and width are less, so can be covered by the microscopical observation visual field simultaneously, the observation visual field is moved to the other end by one end of detection cell gradually along counting scale, avoid omitting the phenomenon of detection or duplicate detection, and moved and the count detection error that causes by microorganism, so workable, there is outstanding substantive distinguishing features with significant progressive with respect to existing slide glass.
Brief description of the drawings
Accompanying drawing 1 is plan structure schematic diagram of the present invention.
Accompanying drawing 2 are accompanying drawings 1 of the present invention along A-A to cross-sectional view.
Accompanying drawing 3 are accompanying drawings 1 of the present invention along B-B to cross-sectional view.
Description of reference numerals is as follows:
Carry glass body 1, marking plate 2, sample liquid inlet 3, sample liquid pond 4, counting scale 5.
Embodiment
Below in conjunction with embodiment and accompanying drawing, technical scheme of the present invention is described in further detail:
In year glass body (1) plane, a side is provided with marking plate (2), opposite side is provided with the sample liquid pond (4) of 4 elongated shapes, its width is that 1-2mm, capacity are 20-50ul, counting scale (5) is carved with in bottom, the termination in each sample liquid pond (4) is provided with circular sample liquid inlet (3), diameter is greater than the width in sample liquid pond (4) and connects with sample liquid pond (4), and each its width of sample liquid pond (4) is all not identical in limited range with capacity.
When the width in sample liquid pond (4) can ensure observation sample liquid, can be covered by the observation visual field of low power opticmicroscope (40-200 is doubly) or stereoscope (10-50 is doubly), the data of its detection are calculated the density that detects microorganism according to following formula:
Density (individual/ml)=counting microbial count (individual)/sample liquid pond volume (ml) of microorganism.
Using before the present invention, clean and dry and on marking plate, carry out institute's sample measuring liquid mark with clear water.When use, with glue head dropper or liquid-transfering gun by the hanging drop of large-scale microorganism to be checked in sample liquid inlet, after suspension flows into sample liquid pond and liquid level and flushes with a year glass surface, covered is also placed in low power opticmicroscope or stereoscopic Microscopic observation.When observation, single sample liquid pond is placed in the observation visual field, and moved gradually to the other end by the one end in sample liquid pond along counting scale, avoid omitting the phenomenon of detection or duplicate detection, and moved and the count detection error that causes by microorganism, after counting finishes, slide glass is cleaned up, properly preserve.
Claims (5)
1. large-scale microorganism quantitative counting microscopic inspection slide glass, is characterized in that technical scheme is as follows:
In year glass body (1) plane, a side is provided with marking plate (2), opposite side is provided with the sample liquid pond (4) of 2-4 elongated shape, its width is 0.5-3mm, capacity is 10-100ul, counting scale (5) is carved with in bottom, the termination in each sample liquid pond (4) is provided with circular sample liquid inlet (3), and diameter is greater than the width in sample liquid pond (4) and connects with sample liquid pond (4).
2. large-scale microorganism quantitative counting microscopic inspection slide glass according to claim 1, is characterized in that: in year glass body (1) plane, opposite side is provided with the sample liquid pond (4) of 4 elongated shapes, and its width is that 1-2mm, capacity are 20-50ul.
3. large-scale microorganism quantitative counting microscopic inspection slide glass according to claim 1, is characterized in that: each its width of sample liquid pond (4) is all not identical in limited range with capacity.
4. large-scale microorganism quantitative counting microscopic inspection slide glass according to claim 1, is characterized in that: the width of sample liquid pond (4) can be covered by the observation visual field of low power opticmicroscope or stereoscope can ensure observation sample liquid time.
5. large-scale microorganism quantitative counting microscopic inspection slide glass according to claim 1, is characterized in that: the data of its detection are calculated the density that detects microorganism according to following formula:
Density (individual/ml)=counting microbial count (individual)/sample liquid pond volume (ml) of microorganism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410313573.XA CN104073433A (en) | 2014-07-23 | 2014-07-23 | Large quantitatively counting microscope detection glass slide for microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410313573.XA CN104073433A (en) | 2014-07-23 | 2014-07-23 | Large quantitatively counting microscope detection glass slide for microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104073433A true CN104073433A (en) | 2014-10-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410313573.XA Pending CN104073433A (en) | 2014-07-23 | 2014-07-23 | Large quantitatively counting microscope detection glass slide for microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104073433A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105158890A (en) * | 2015-09-18 | 2015-12-16 | 浙江大学 | Adjustable anti-shaking anti-displacement lofting platform |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817495A (en) * | 2010-03-25 | 2010-09-01 | 湖南大学 | Micro fluid control chip and preparation method and application thereof |
| CN202994647U (en) * | 2013-01-15 | 2013-06-12 | 中国科学院物理研究所 | Multi-channel flow sample pool used for microscope |
| CN203070345U (en) * | 2012-12-28 | 2013-07-17 | 甘肃农业大学 | Nematode counting plate |
| CN203191629U (en) * | 2013-04-08 | 2013-09-11 | 张海艳 | Microbiological test slide |
-
2014
- 2014-07-23 CN CN201410313573.XA patent/CN104073433A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817495A (en) * | 2010-03-25 | 2010-09-01 | 湖南大学 | Micro fluid control chip and preparation method and application thereof |
| CN203070345U (en) * | 2012-12-28 | 2013-07-17 | 甘肃农业大学 | Nematode counting plate |
| CN202994647U (en) * | 2013-01-15 | 2013-06-12 | 中国科学院物理研究所 | Multi-channel flow sample pool used for microscope |
| CN203191629U (en) * | 2013-04-08 | 2013-09-11 | 张海艳 | Microbiological test slide |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105158890A (en) * | 2015-09-18 | 2015-12-16 | 浙江大学 | Adjustable anti-shaking anti-displacement lofting platform |
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Application publication date: 20141001 |