CN203070345U - Nematode counting plate - Google Patents
Nematode counting plate Download PDFInfo
- Publication number
- CN203070345U CN203070345U CN 201220738292 CN201220738292U CN203070345U CN 203070345 U CN203070345 U CN 203070345U CN 201220738292 CN201220738292 CN 201220738292 CN 201220738292 U CN201220738292 U CN 201220738292U CN 203070345 U CN203070345 U CN 203070345U
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- nematode
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- microslide
- slide
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Abstract
The utility model discloses a nematode counting plate, which comprises a slide. Two parallel grooves are arranged on the slide. The parallel grooves are arranged on the slide, the tested liquid is limited inside the grooves, the width of the groove is just within the vision field, observation is clear only by moving the slide in one direction, and the small groove is internally provided with a scale with 3mm as a unit, is also within the vision field and serves as a reference, thereby avoiding counting missing and repetition.
Description
Technical field
The utility model relates to the bio-instruments technical field, especially a kind of nematodal accounting plate.
Background technology
Root-knot nematode belongs to soil sedentariae endoparasitism nematode and belongs to root-knot nematode section Meloidogyne.This disease found the cucumber in Britain greenhouse by Berkeley at first in 1855, was one of found plant nematode disease early.Root-knot nematode is one of important causal organism that harms the crops, and it also is the widest, the heaviest plant nematode of causing harm that distributes, and has caused the concern of countries in the world, also is one of current China most important crops cause of disease nematode.The root-knot nematode of report has kind more than 80 in the world at present.Main parasitic is on more than 3000 kind of host such as cereal crops, industrial crops, fruit tree, vegetables, ornamental plant and weeds.China is nearly surpass 400,000 hectares protection be subjected to the harm of root-knot nematode, the annual loss of causing harm to various vegetables surpasses 3,000,000,000 yuan, and can rise with the amplification of annual 4% ~ 9% in following 10 years.Because the uniqueness of root-knot nematode harm, people are later to the understanding of root-knot nematode harm, the control slower development of nematode.There were the serious generation of root knot nematode disease and popular report in ground such as Heilungkiang, Liaoning, Beijing, Henan, Hubei, Jiangsu, Yunnan and Hainan, and it is significant therefore root knot nematode disease to be carried out systematic research.
The statistics of nematode is to draw a certain amount of nematode aqueous solution with liquid-transfering gun at present, and the mode of the line of striking is drawn on microslide, moves up and down then to observe counting under 40 times of mirrors.The waterline of Huaing is random like this, and area is big, moves up and down slide about will be careful under 40 times of mirrors, and the ability number is clear, also might leak number and repeat wherein zone.
The utility model content
The utility model proposes a kind of nematodal accounting plate at the deficiencies in the prior art, the convenient observation and accurate counting.
In order to realize above-mentioned utility model purpose, the utility model provides following technical scheme: a kind of nematodal accounting plate, comprise microslide, and this microslide is provided with the groove that twice are parallel to each other.
Further, this microslide is 76 mm * 25mm * 1.5mm.
Further, this groove is wide 3.6mm, degree of depth 0.5mm, long 60mm.
Further, two grooves are at a distance of 7.8mm.
Further, be provided with the scale mark that 3mm is a unit in this groove vertically.
Compared with prior art, the utlity model has following advantage: at microslide parallel groove is set, tracer liquid is confined in the groove, and the width of groove is just within sweep of the eye, only move to a direction and can observe clearly, having in the sulculus with 3mm is the scale of a unit, also within sweep of the eye, as a reference, leakage number and repetition have been avoided.
Description of drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
Below in conjunction with accompanying drawing the utility model is described in detail, the description of this part only is exemplary and explanatory, should any restriction not arranged to protection domain of the present utility model.
A kind of nematodal accounting plate as shown in Figure 1 comprises microslide 1, and this microslide 1 is provided with the groove 11 that twice are parallel to each other.Carve the groove 11 of the wide 3.6mm of twice, the about 0.5mm of the degree of depth at 76 mm * 25mm * 1.5mm microslide 1, the groove 11 long 60mm that are.Two grooves 11 are at a distance of 7.8mm, apart from microslide 1 two ends 8mm, apart from both side edges 5mm.In groove 11, be that a unit engraves scale with 3mm.
The nematode aqueous solution that will count, draw 100ul with liquid-transfering gun, with the method for line liquid is injected wherein in the groove 11 then, same method is also injected liquid in second groove 11, under 40 power microscopes, observe counting, move left and right slide 1 just can be fast, the perfect number nematode number in the liquid in the sulculus clearly wherein, the nematode number in denumerable clear another sulculus of same method.
Under 40 power microscopes, the diameter in the visual field is 4mm, and the wide 3.6mm of sulculus within field range, need not move up and down microslide, and directly move left and right gets final product.Because the volume of groove is 60mm * 3.6mm * 0.5mm=108mm
3100ul, 100ul liquid can be not excessive in groove, and groove is moved on under 40 times of mirrors, and move left and right microslide 1 can go out the nematode in the liquid in the sulculus by observed number.
Adopting the utility model device can count the number of nematode fast and accurately under 40 power microscopes, can count nematode more clear, accurately and rapidly, avoided repeating and forgetting, is effective, simple, fast device therefore.
Claims (5)
1. a nematodal accounting plate comprises microslide, it is characterized in that: this microslide is provided with the groove that twice are parallel to each other.
2. nematodal accounting plate according to claim 1, it is characterized in that: this microslide is 76 mm * 25mm * 1.5mm.
3. as nematodal accounting plate as described in the claim 2, it is characterized in that: this groove is wide 3.6mm, degree of depth 0.5mm, long 60mm.
4. as nematodal accounting plate as described in claim 2 or 3, it is characterized in that: two grooves are at a distance of 7.8mm.
5. as nematodal accounting plate as described in the claim 4, it is characterized in that: be provided with the scale mark that 3mm is a unit in this groove vertically.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220738292 CN203070345U (en) | 2012-12-28 | 2012-12-28 | Nematode counting plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220738292 CN203070345U (en) | 2012-12-28 | 2012-12-28 | Nematode counting plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203070345U true CN203070345U (en) | 2013-07-17 |
Family
ID=48769110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220738292 Expired - Fee Related CN203070345U (en) | 2012-12-28 | 2012-12-28 | Nematode counting plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203070345U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073433A (en) * | 2014-07-23 | 2014-10-01 | 西北农林科技大学 | Large quantitatively counting microscope detection glass slide for microorganisms |
| CN107279066A (en) * | 2017-06-28 | 2017-10-24 | 安徽科技学院 | A kind of volume quantitative investigation method of small insects population quantity |
| CN107410222A (en) * | 2017-06-28 | 2017-12-01 | 安徽科技学院 | A kind of investigation method of weighing of hemipteran population quantity |
-
2012
- 2012-12-28 CN CN 201220738292 patent/CN203070345U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073433A (en) * | 2014-07-23 | 2014-10-01 | 西北农林科技大学 | Large quantitatively counting microscope detection glass slide for microorganisms |
| CN107279066A (en) * | 2017-06-28 | 2017-10-24 | 安徽科技学院 | A kind of volume quantitative investigation method of small insects population quantity |
| CN107410222A (en) * | 2017-06-28 | 2017-12-01 | 安徽科技学院 | A kind of investigation method of weighing of hemipteran population quantity |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130717 Termination date: 20151228 |
|
| EXPY | Termination of patent right or utility model |