CN104070062A - Microbial in-situ remediation method of heavy metal contaminated soil - Google Patents
Microbial in-situ remediation method of heavy metal contaminated soil Download PDFInfo
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Abstract
The invention relates to a microbial in-situ remediation method of heavy metal contaminated soil. The method comprises the following steps: (1) flora culture; (2) flora activation; (3) microbial growth agent preparation; (4) nutrient solution preparation; (5) primary pouring; and (6) remediation. According to the microbial in-situ remediation method of heavy metal contaminated soil, heavy metal microbial degradation floras and a microbial growth accelerator serve as the main ingredients, certain microbial nutrients are replenished and the microbial growth accelerator is added to promote high speed reproduction of heavy metal microbial transformation floras while preferably putting the heavy metal microbial transformation floras in the soil, thus greatly improving the transformation and degradation efficiency of the heavy metal elements in the soil, solving the problems of long time and slowness in effect taking in a microbial in-situ remediation method of heavy metal contaminated soil and enabling the microbial in-situ remediation technology to become the reliable, efficient and operable heavy metal contaminated soil control means.
Description
Technical field
The invention belongs to contaminated soil and administer field, relate to microorganism in situ reparation, especially a kind of microorganism in situ restorative procedure of heavy-metal contaminated soil.
Background technology
Soil pollution is apparently higher than original content of soil, to surpass the self-purification capacity of soil because mankind's activity causes the content of some harmful components, cause that thus composition of the soil, 26S Proteasome Structure and Function change, harmful substance or its catabolite be accumulation gradually in soil, reaches the degree being detrimental to health.Wherein, heavy metal pollution of soil problem is one of the most outstanding soil pollution problem, and it is mainly derived from sewage irrigation, industrial residue, municipal refuse, trade waste stacking and atmospheric sedimentation.Heavy metal pollution of soil mainly comprises the significant elements of bio-toxicity such as mercury (Hg), cadmium (Cd), plumbous (Pb), chromium (Cr) and metalloid arsenic (As), and the element such as zinc (Zn), copper (Cu), nickel (Ni) that has certain toxicity, generally can only there is transformation and the migration of form, hard degradation in vertical distribution.
At present, traditional methods for curing heavy metal contamination in soil mainly contains: the engineering measure based on mechanical-physical or physical and chemical principle, as soil replacement method, isolation method, ablution, heat treating process, electrochemical process etc.; Ameliorative measure based on pollutant chemistry behavior, reduces water-soluble, diffusivity and the biological effectiveness of soil pollutant as added modifying agent, inhibitor.
Microorganism in situ recovery technique refers to the biological control measure that utilizes specific microorganism absorption, conversion, removing or degraded heavy metal pollution of soil thing to realize soil sanitation.This technology can make heavy metal contaminants remove from soil, less to surrounding environment influence, can not produce secondary pollution, the huge soil remediation potentiality that have.But the microorganism recovery technique of existing heavy-metal contaminated soil has some limitations, as microorganism repair the duration long, take effect slow, the large and reparation etc. that is not suitable for severe contamination soil affected by environment.
Research shows, the common factor that restriction heavy metal pollution place microorganism in situ is repaired process is that contaminated site lacks enough nutrition conventionally for external microorganism or indigenous microorganism growth, so that the heavy metal that these microorganisms possess self is administered potentiality and is not in full use, and fails to become comparatively desirable Remediation Methods for Heavy Metal Contamination of Soil.
Through retrieval, find one piece of patent documentation relevant to heavy metal soil remediation, the Chinese patent that publication number is CN1544167 discloses a kind of biological in-situ restorative procedure of copper polluted soil.The method is by plantation elsholtzia splendens, utilize the root system of elsholtzia splendens to absorb in a large number the copper in soil, and upwards carry and be transported to overground part, regularly or depending on elsholtzia splendens growing height gather in overground part and removed, and from soil, take away a large amount of copper, realize the biological in-situ reparation of copper polluted soil.
Through contrast, above-mentioned patent and teachings herein have relatively big difference.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art part, a kind of Soil Microorganism microorganism in situ restorative procedure growth and the heavy-metal contaminated soil that degradation rate is higher that promotes is provided.
The present invention is achieved through the following technical solutions goal of the invention:
A microorganism in situ restorative procedure for heavy-metal contaminated soil, the steps include:
(1) bacteria group culture: selection can transform the microbial degradation flora body of heavy metal characteristic, adopts the mode of solid culture, and the wheat bran of take is cultivated domestication as carrier, gets the powdered substance that bacterial product mixing in the middle of it also forms after mummification;
(2) flora activates: the powdered substance by step in is (1) put into water, soaks 0.5 ~ 2h, removes wheat bran and obtains the flora liquid after activation;
(3) growth of microorganism agent preparation: compound amino acid, composite trace element, activating enzymes and humic acid are put into water, stir, obtain growth of microorganism agent;
(4) compounding: add microbial nutrition element in growth of microorganism agent, add water and diluted, obtain growth of microorganism agent nutrient solution;
(5) just throw: by disposable the pouring in contaminated by heavy metals soil of flora liquid after activating, the growth of microorganism agent nutrient solution of getting gross weight 20% is sprayed in the soil of heavy metal pollution, plowed soils, fully mixes flora liquid, growth of microorganism agent nutrient solution contaminated soil;
(6) repair: after just throwing, growth of microorganism agent nutrient solution is divided into 3 ~ 6 equal portions, within every 10 days, in contaminated soil, evenly spray a growth of microorganism agent nutrient solution, during each sprinkling, augment moisture and the contaminated soil of the turning over oxygen that exposes to the sun, each heavy metal element in contaminated soil can be down in the index that GB requires.
And step (1) described microbial degradation flora body is avirulence bacterial community and heterotroph micropopulation.
And step (2) described powdered substance and water weight ratio is 1 ~ 2:10.
And the step (3) weight ratio of described compound amino acid, composite trace element, activating enzymes, humic acid and water is: 1:1:1:2:4 ~ 5.
And step (4) described microbial nutrition element, growth of microorganism agent and the water weight ratio that feeds intake is: 1:2 ~ 3:20 ~ 25.
And step (4) described microbial nutrition element comprises organic matter and inorganic salts, wherein organic matter comprises 3Kg protein and 1.5Kg sugar, and inorganic salts are 2Kg, and its weight ratio is protein: sugar: inorganic salts=3:1.5:2.
And component and parts by weight that described inorganic salts are included are respectively:
0.1 part～0.2 part, sodium chloride, 0.1 part～0.2 part of ammonia, 0.2 part～0.4 part, ammonium sulfate, 0.2 part～0.4 part of potassium dihydrogen phosphate, 0.2 part～0.4 part of dipotassium hydrogen phosphate, 0.06 part～0.12 part, magnesium sulfate, 0.05 part～0.1 part, zinc sulfate, 0.03 part～0.06 part, calcium chloride, 0.02 part～0.04 part, calcium nitrate, 0.02 part～0.04 part, ferric sulfate, 0.02 part～0.04 part of manganese sulfate.
And step (5) described contaminated by heavy metals thickness of soil is 30cm, area is 100m
2.
The present invention has following advantage and good effect:
1, in the microorganism in situ restorative procedure of heavy-metal contaminated soil provided by the invention by heavy metal microbial degradation flora, growth of microorganism agent is as main component, supplemented again certain microbial nutrition element, dropping into preferred heavy metal microbial conversion flora in soil when, increasing growth of microorganism agent impels it to breed at a high speed, thereby conversion and the degradation efficiency of heavy metals in soil have greatly been improved, the time having solved in heavy-metal contaminated soil microorganism in situ restorative procedure is long, slow problem takes effect, microorganism in situ recovery technique is become reliably, efficiently, exercisable heavy-metal contaminated soil governing measure.
2, the present invention utilizes the characteristic of microorganism to heavy metal adsorption and conversion heavy metal, realize the microorganism in situ repair of heavy-metal contaminated soil, according to specific using method, this bacterium liquid and growth of microorganism agent and microbial nutrition element being applied to in the soil of heavy metal pollution, can reach degraded heavy metal in soil pollutes, heavy metal ion enters microbial cell, can be by being oxidized, reducing, methylate or demethylation changing into valence stability, the less or nontoxic compound of toxicity, thus the toxic action to organism alleviated.
The specific embodiment
Below by specific embodiment, further narrate the present invention, it should be noted that following examples are illustrative, but not determinate.To those skilled in the art, do not deviating under the prerequisite of essence of the present invention and scope various changes that the material component in these embodiments and consumption are carried out or change and also belong to protection scope of the present invention.
A microorganism in situ restorative procedure for heavy-metal contaminated soil, the steps include:
(1) bacteria group culture: selection can transform avirulence bacterial community and heterotroph micropopulation microbial degradation flora body 1 Kg of heavy metal characteristic, adopt the mode of solid culture, the wheat bran of take is cultivated domestication as carrier, gets its middle bacterial product and mixes the powdered substance forming after also mummification;
(2) flora activates: the powdered substance by step in is (1) put into 10 Kg water, soaks 0.5 ~ 2h, removes wheat bran and obtains the flora liquid after activation;
(3) growth of microorganism agent preparation: 0.4Kg compound amino acid, 0.4Kg composite trace element, 0.4Kg activating enzymes and 0.8Kg humic acid are put into water 18Kg, stir, obtain growth of microorganism agent;
(4) compounding: add 6.5Kg microbial nutrition element in growth of microorganism agent, add 400Kg water and diluted, obtain growth of microorganism agent nutrient solution;
(5) just throw: by the flora liquid after activating, disposable to pour contaminated by heavy metals thickness into be 30cm, and area is 100m
2in soil, the growth of microorganism agent nutrient solution of getting gross weight 20% is sprayed in the soil of heavy metal pollution, and plowed soils fully mixes flora liquid, growth of microorganism agent nutrient solution contaminated soil;
(6) repair: after just throwing, growth of microorganism agent nutrient solution is divided into 3 ~ 6 equal portions, within every 10 days, in contaminated soil, evenly spray a growth of microorganism agent nutrient solution, during each sprinkling, augment moisture and the contaminated soil of the turning over oxygen that exposes to the sun, each heavy metal element in contaminated soil can be down in the index that GB requires.
Mentioned microorganism nutrient comprises organic matter and inorganic salts, wherein organic matter comprises 3Kg protein and 1.5Kg sugar, inorganic salts are 2Kg, its component is sodium chloride, ammonia, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, zinc sulfate, calcium chloride, calcium nitrate, ferric sulfate, manganese sulfate, each weight percentages of components is: sodium chloride 0.1%～0.2%, ammonia 0.1%～0.2%, ammonium sulfate 0.2%～0.4%, potassium dihydrogen phosphate 0.2%～0.4%, dipotassium hydrogen phosphate 0.2%～0.4%, magnesium sulfate 0.06%～0.12%, zinc sulfate 0.05%～0.1%, calcium chloride 0.03%～0.06%, calcium nitrate 0.02%～0.04%, ferric sulfate 0.02%～0.04%, manganese sulfate 0.02%～0.04%.
Simulated experiment:
The microorganism in situ restorative procedure of above-mentioned heavy-metal contaminated soil is applied to indoor heavy metal simulating pollution soil.
Experiment soil sample is milled with agate mortar, crosses after 60 mm aperture sieve for Heavy Metals and complete analysis.Mensuration obtains for examination physiochemical properties of soil: the content of organic matter 5.55 g/kg, and cation exchange capacity (CEC) 20.0cmol/kg, glutinous grain, powder and sand grain content are respectively 220 g/kg, 640 g/kg and 140 g/kg, pH value 6.10, quality is the glutinous earth of powder.
Result of the test is found:
For being subject to Hg contaminated soil, degrade after one month, the Hg in soil is removed 55%; After two months, the Hg in soil is removed 76%; After three months, the Hg in soil is removed 99%.
For being subject to Cr contaminated soil, degrade after one month, the Cr in soil is removed 35%; After two months, the Cr in soil is removed 56%; After three months, the Cr in soil is removed 73%.
Soil (mass concentration of total Pb, total Zn, total Cu and total Cd is respectively 44.80 mg/kg, 2.02mg/kg, 19.77mg/kg and 2.22 mg/kg) for slight comprehensive heavy metal pollution, degrade after three months, in soil, the content of Pb, Zn, Cu, Cd has declined respectively 68%, 92%, 86%, 59%.
Site test:
The microorganism in situ restorative procedure of above-mentioned heavy-metal contaminated soil is applied to certain Electroplate Factory's contaminated site seriously polluted area region soil, heavy metal in soil full dose and each form content are administered to experiment.
This soil is the most serious with chromium and nickel contamination, soil Cr and nickel content reach respectively 1564.00 mg/kg and 679.00 mg/kg, Soil Copper, zinc and lead content are respectively 297.00 mg/kg, 276.00 mg/kg and 51.40 mg/kg, and copper, chromium, nickel, zinc and plumbous available state ratio are respectively 41.77%, 13.16%, 28.08%, 21.50% and 31.18%.
We distinguish the microorganism in situ restorative procedure operation of heavy-metal contaminated soil, and result shows, better to copper, chromium, nickel and zinc removal effect, clearance is respectively 85.1%, 64.8%, 77.5% and 89.3%.
Claims (8)
1. a microorganism in situ restorative procedure for heavy-metal contaminated soil, is characterized in that: the steps include:
(1) bacteria group culture: selection can transform the microbial degradation flora body of heavy metal characteristic, adopts the mode of solid culture, and the wheat bran of take is cultivated domestication as carrier, gets the powdered substance that bacterial product mixing in the middle of it also forms after mummification;
(2) flora activates: the powdered substance by step in is (1) put into water, soaks 0.5 ~ 2h, removes wheat bran and obtains the flora liquid after activation;
(3) growth of microorganism agent preparation: compound amino acid, composite trace element, activating enzymes and humic acid are put into water, stir, obtain growth of microorganism agent;
(4) compounding: add microbial nutrition element in growth of microorganism agent, add water and diluted, obtain growth of microorganism agent nutrient solution;
(5) just throw: by disposable the pouring in contaminated by heavy metals soil of flora liquid after activating, the growth of microorganism agent nutrient solution of getting gross weight 20% is sprayed in the soil of heavy metal pollution, plowed soils, fully mixes flora liquid, growth of microorganism agent nutrient solution contaminated soil;
(6) repair: after just throwing, growth of microorganism agent nutrient solution is divided into 3 ~ 6 equal portions, within every 10 days, in contaminated soil, evenly spray a growth of microorganism agent nutrient solution, during each sprinkling, augment moisture and the contaminated soil of the turning over oxygen that exposes to the sun, each heavy metal element in contaminated soil can be down in the index that GB requires.
2. the microorganism in situ restorative procedure of heavy-metal contaminated soil according to claim 1, is characterized in that: step (1) described microbial degradation flora body is avirulence bacterial community and heterotroph micropopulation.
3. the microorganism in situ restorative procedure of heavy-metal contaminated soil according to claim 1, is characterized in that: step (2) described powdered substance and water weight ratio is 1 ~ 2:10.
4. the microorganism in situ restorative procedure of heavy-metal contaminated soil according to claim 1, is characterized in that: the step (3) weight ratio of described compound amino acid, composite trace element, activating enzymes, humic acid and water is: 1:1:1:2:4 ~ 5.
5. the microorganism in situ restorative procedure of heavy-metal contaminated soil according to claim 1, is characterized in that: step (4) described microbial nutrition element, growth of microorganism agent and the water weight ratio that feeds intake is: 1:2 ~ 3:20 ~ 25.
6. the microorganism in situ restorative procedure of heavy-metal contaminated soil according to claim 1, it is characterized in that: step (4) described microbial nutrition element comprises organic matter and inorganic salts, wherein organic matter comprises 3Kg protein and 1.5Kg sugar, inorganic salts are 2Kg, and its weight ratio is protein: sugar: inorganic salts=3:1.5:2.
7. the microorganism in situ restorative procedure of heavy-metal contaminated soil according to claim 6, is characterized in that: component and parts by weight that described inorganic salts are included are respectively:
0.1 part～0.2 part, sodium chloride, 0.1 part～0.2 part of ammonia, 0.2 part～0.4 part, ammonium sulfate, 0.2 part～0.4 part of potassium dihydrogen phosphate, 0.2 part～0.4 part of dipotassium hydrogen phosphate, 0.06 part～0.12 part, magnesium sulfate, 0.05 part～0.1 part, zinc sulfate, 0.03 part～0.06 part, calcium chloride, 0.02 part～0.04 part, calcium nitrate, 0.02 part～0.04 part, ferric sulfate, 0.02 part～0.04 part of manganese sulfate.
8. the microorganism in situ restorative procedure of heavy-metal contaminated soil according to claim 1, is characterized in that: step (5) described contaminated by heavy metals thickness of soil is 30cm, and area is 100m
2.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105750314A (en) * | 2016-05-10 | 2016-07-13 | 湖南农业大学 | Method for improving capability of endives for removing cadmium from heavy-metal-polluted soil by using fertilization and harvesting techniques |
| CN107057708A (en) * | 2017-02-22 | 2017-08-18 | 谢松甫 | A kind of soil remediation medicament |
| CN108655161A (en) * | 2018-05-25 | 2018-10-16 | 四川川能环保科技有限公司 | A kind of biology in situ renovation method of organic material contaminated soil |
| CN110721996A (en) * | 2019-10-29 | 2020-01-24 | 广州国苑规划设计有限公司 | Remediation method for contaminated soil plants |
| CN114101319A (en) * | 2021-11-19 | 2022-03-01 | 福建省环境保护设计院有限公司 | Nutrition layer spreading machine for treating severe chromium-contaminated soil and soil treatment method |
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- 2013-03-29 CN CN201310108243.2A patent/CN104070062A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105750314A (en) * | 2016-05-10 | 2016-07-13 | 湖南农业大学 | Method for improving capability of endives for removing cadmium from heavy-metal-polluted soil by using fertilization and harvesting techniques |
| CN107057708A (en) * | 2017-02-22 | 2017-08-18 | 谢松甫 | A kind of soil remediation medicament |
| CN108655161A (en) * | 2018-05-25 | 2018-10-16 | 四川川能环保科技有限公司 | A kind of biology in situ renovation method of organic material contaminated soil |
| CN110721996A (en) * | 2019-10-29 | 2020-01-24 | 广州国苑规划设计有限公司 | Remediation method for contaminated soil plants |
| CN110721996B (en) * | 2019-10-29 | 2022-02-01 | 广州国苑规划设计有限公司 | Remediation method for contaminated soil plants |
| CN114101319A (en) * | 2021-11-19 | 2022-03-01 | 福建省环境保护设计院有限公司 | Nutrition layer spreading machine for treating severe chromium-contaminated soil and soil treatment method |
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Application publication date: 20141001 |