CN110721996B - Remediation method for contaminated soil plants - Google Patents
Remediation method for contaminated soil plants Download PDFInfo
- Publication number
- CN110721996B CN110721996B CN201911034192.7A CN201911034192A CN110721996B CN 110721996 B CN110721996 B CN 110721996B CN 201911034192 A CN201911034192 A CN 201911034192A CN 110721996 B CN110721996 B CN 110721996B
- Authority
- CN
- China
- Prior art keywords
- contaminated soil
- soil
- remediation method
- fungus
- remediation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a remediation method for contaminated soil plants, applying a remediation composition to soil, and applying a barrier to the surface of the soil within the application range; the repair composition comprises the following components: protein, lignocellulose, fungi, legume shrub grinds; wherein the protein is calculated on a dry weight basis; the water content of the lignocellulose is less than 5%; the application amount of the repairing composition is 0.04-0.5kg/m2。
Description
Technical Field
The invention belongs to the technical field of soil remediation, and particularly relates to a remediation method for contaminated soil plants.
Background
With the rapid development of industrial and agricultural production, the natural environment for human survival is increasingly seriously affected by various adverse factors. The interference of human activities is the first to come. The influence of human activities on the soil environment dates back to ancient times, but the influence range is small at that time. The impact of human activities on the soil environment has not begun to grow dramatically in europe until after the industrial revolution.
After China is established since new China, with the development of industry, a large amount of waste water, waste gas and solid waste are discharged to the environment to cause environmental pollution, including soil pollution. The external toxic and harmful pesticides, petroleum, heavy metals and acidic substances destroy the dynamic balance of substances in the soil and the inherent ecosystem, or the fertility of the same plant is reduced because the same plant is continuously planted in the same piece of soil. Soil pollution is increasingly paid more attention to people as an invisible pollution. Relevant experts point out that although China achieves certain performance in environmental governance, the problem of soil pollution is still developing continuously, and high attention and attention of relevant departments should be drawn.
Disclosure of Invention
The invention aims to provide a remediation method for contaminated soil plants.
The technical scheme adopted by the invention for solving the technical problem is as follows:
a remediation process for contaminated soil plants by applying a remediation composition to the soil and applying a barrier to the surface of the soil within the application zone;
the repair composition comprises the following components in percentage by mass:
wherein the protein is calculated on a dry weight basis; the water content of the lignocellulose is less than 5%; the application amount of the repairing composition is 0.04-0.5kg/m2；
Further, the plant species of the leguminous shrub ground product includes at least one of mesquite, cassia tora, jatropha, amorpha fruticosa, faecium procumbens, and silvery.
Further, the protein includes potato protein and/or wheat protein.
Further, fungi are of the species that produce lignin peroxidase.
Further, the fungus is a white rot fungus.
Further, a method for restoring a plant used for contaminated soil, characterized by: the fungi include: at least one of Phanerochaete chrysosporium, Quercus labyrinthi, Profenoporus cinnabarinus, Alternaria mucilaginosa, Ceriporiopsis carbonaria, Trichoderma harzianum, Aspergillus oryzae, and Coriolus versicolor.
Further, the leguminous shrub grind is the root and/or stem of a plant.
Further, the barrier has an oxygen transmission rate of 2000 ml/h.m or less2。
The fungi need to be wrapped by lignocellulose, and the water content is controlled below 5%, so that the fungi are in a nutrient state. This is to keep the survival time of the fungus as delayed as possible before release and to avoid that the fungus is in the propagules and cannot reach the desired function.
At the same time, lignocellulose is used as a fungus in order to be able to function as quickly as possible after release. Lignocellulose can be derived from byproducts left after the harvest of various plants, and residues of paper industry and lumbering industry; such as tree trunks, spent coffee grounds, waste paper, fallen leaves, fallen flowers, corner branches, bark, and the like.
The fungus used in the present invention is desirably a fungus capable of producing lignin peroxidase, preferably white rot fungus. The function of the fungus is that the white rot fungus can effectively lignocellulose so as to improve soil fertility and degrade environmental pollutants (Ashher M, Bhatti HN, Ashraf M and Legge RL. recent definitions in biological yield of industrial polutants by white rot fungus and the same enzyme system biological yield.2008, 19: 771-one 783), meanwhile, the fungus has the function of enriching heavy metals, and the white rot fungus can effectively inhibit the growth of nematodes in soil, especially root-knot nematodes; the fungi have certain loosening effect on soil in the process of hypha growth or fruiting body growth. And the lignocellulose has wide source and low cost, and can not cause new pollution sources.
The legume shrub grinds used in the present invention are the remaining parts of the legumes after harvest, mainly the roots and stems. The leguminous shrub grinding material can be used for continuing to grow after the white rot fungi consume the wrapped lignocellulose, and meanwhile, the physical properties of the soil can be changed according to the difference of the sizes of the grinding material. Soil which is usually polluted or/and has reduced fertility has the problems of hardening and over-hardening. When fungi cannot consume all the leguminous shrub ground materials, the rest ground materials can exist in soil for a long time, so that the water can be temporarily stored while hardening is prevented until the ground materials are utilized by other decomposers or plants.
Meanwhile, according to the records of 8 arbuscular mycorrhizal fungi of leguminous shrub cultivars and distribution, Wutao Yao Hongyan and the like, grass science 2016,33(02),210-218, the roots of leguminous shrub plants form the symbiotic relationship of AM fungi-leguminous plants-rhizobia, and the rhizobia is an important azotobacter and has a great improvement relationship on soil fertility, and the AM fungi can improve the resistance of the plants to diseases, barrenness, salt stress, aluminum toxicity and pollution. The rhizobia and AM fungi which are attached to the leguminous shrub grinding material have great auxiliary effects on the fertility restoration and pollution remediation of soil in the invention.
Meanwhile, in order to kill nematodes, fungi, bacteria and the like which are originally present in the polluted soil, the method is carried out by using proteins and paving a barrier to isolate oxygen, and the fungi carried by the remediation composition are wrapped in lignocellulose and are in a dormant state; after the barrier is removed, the biological bacterial colony reconstructed in the polluted soil can be quickly propagated and diffused to become a dominant species, so that the organisms which are harmful to the soil fertility are pressed. Meanwhile, according to the record in the feasibility research of the application of the protein in the biological evaluation of the polluted soil, Zhang, Zhejiang university, the protein can repair heavy metals in the soil.
The invention mainly treats plants and microorganisms and then prepares the plants and the microorganisms into preparations so as to achieve the aim of improving or repairing soil.
Detailed Description
The following specific examples are provided to further illustrate the present invention so that those skilled in the art may better understand the invention and practice it, but the examples are not intended to limit the invention.
The plants used in the present invention include: prosopis pubescens, Cassia tora, Dendrolobium triamgulare, Amorphophallus Amorpha Amorphophallus fructico, Crotalaria pallida, Leucaena leucocepha.
The fungi used in the present invention include: phanerochaete chrysosporium, Erythrophora Quercaria Daedalea quercina, Pycnoporus cinnabarinus, Merulasprellus tremenus, Trichoderma harzianum, Aspergillus oryzae, Trametes versicolor, Ceriporiopsis subvermispora.
The plants and fungi can be obtained by the public, including but not limited to the on-line platforms of Ali baba, China biological equipment network, chemical engineering instrument network, syringone and the like; or an off-line brick and mortar store with sales qualifications.
Example 1: influence of formula proportion on land pollution remediation
The repair composition was formulated as follows
Table 1: formulation proportion table
The units in table 1 are: % of the total weight of the composition.
The experimental steps are as follows:
step0, measuring and recording the content of heavy metals, and dividing areas according to the remediation compositions with different numbers, wherein each area is 20X20 m; all areas are set within one mu of land in the same area.
Step1 applying the prepared repairing composition to the soil after turning the soil for 10cm, wherein the release amount is 0.3kg/m2；
Step 2: and ploughing the soil again after releasing, wherein the depth is 20 cm.
Step3, covering a plastic film on the soil surface at the same time, wherein the oxygen transmission rate of the plastic film is less than 2000 ml/h.m2。
Step 4: after one week, the film is opened, the heavy metal content is detected again after 12 months, and vegetation above the soil is not treated in the experimental process and is allowed to grow.
The experimental site: southern sand area of Guangzhou city
Experiment time: 3 months in 2018-3 months in 2019
In monitoring the heavy metal content, five spots were randomly selected in each region and individually detected and the average value was recorded as data in table 2 below. The units in the table are: mg/kg.
Table 2: formulation ratio experimental data
| Serial number | Chromium Cr | Arsenic As | Copper Cu | Lead Pb | Ni | Cadmium Cd |
| Standard value < > | 150 | 40 | 50 | 90 | 70 | 0.3 |
| 1 (before experiment) | 126.1 | 152.2 | 460.2 | 210.5 | 139.2 | 2.3 |
| 1 (after experiment) | 10.8 | 0.3 | 80.2 | 21.0 | 12.0 | 0.2 |
| 2 (before experiment) | 128.0 | 149.8 | 458.5 | 209.9 | 140.1 | 2.2 |
| 2 (after experiment) | 12.7 | 0.5 | 76.2 | 17.6 | 11.9 | 0.1 |
| 3 (before experiment) | 126.9 | 151.1 | 459.1 | 210.1 | 139.8 | 2.3 |
| 3 (after experiment) | 8.2 | 2.1 | 52.4 | 16.4 | 13.6 | 0.1 |
| 4 (before experiment) | 127.5 | 150.3 | 459.8 | 209.6 | 139.7 | 2.1 |
| 4 (after experiment) | 4.3 | 1.1 | 37.6 | 23.1 | 14.9 | Trace amount of |
| 5 (before experiment) | 127.9 | 151.2 | 460.9 | 210.3 | 138.9 | 2.2 |
| 5 (after experiment) | 9.6 | 2.3 | 46.7 | 19.2 | 9.8 | 0.2 |
| 6 (before experiment) | 126.9 | 150.6 | 461.0 | 201.1 | 140.6 | 2.0 |
| 6 (after experiment) | 25.7 | 4.3 | 39.8 | 16.4 | 11.4 | Trace amount of |
The determination standard is as follows: soil environment quality agricultural land soil pollution risk control standard (GB15618-2018) takes a determination method in the standard as a standard, and the standard values in the table 2 take agricultural land soil pollution risk screening values (basic items) in the table 1 in the standard, wherein the pH value is more than 5.5 and less than or equal to 6.5, and the other standard values are taken as a standard.
According to the data, the invention comprises the following steps:
1. the potato protein or the wheat protein or the mixture of the two has little influence on the repairing effect.
2. The fungus species have little influence on the repairing effect, and the effect can be achieved by white rot fungi, but the absorption effect on each heavy metal is different.
3. The mass ratio of lignocellulose to fungi is 4: preferably 1.
4. The plant variety of the leguminous shrub ground substance has little influence on the repairing effect by using single variety or mixed variety grinding, and can achieve the effect.
Example 2 Effect of remediation composition Release on land pollution remediation
According to the formula with the number 1 in the table 1, extra areas are selected as experimental sites again in the same place and the same soil range at the same time in the example 1, each area is 20X20m, and the experimental method is the same as that of the example 1. Tables 3 and 4 are obtained.
Table 3: repairing composition delivery
| Release amount/number | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| Unit: kg/m2 | 0.02 | 0.04 | 0.1 | 0.2 | 0.3 | 0.4 | 0.5 | 0.6 |
Table 4: effect of remediation composition release on land pollution remediation
As can be seen from the above table, when the amount of the released substance is less than 0.04kg/m2Although the repair effect is achieved, the repair is slow; and the release amount is more than 0.05kg/m2The repair effect slightly fluctuates but the whole effect is still goodWithin the range of (1), no better effect is obtained by increasing the dosage continuously after the requirement is met.
For the restoration of the soil fertility, the invention does not detect all technical parameters. However, by visual inspection, the vegetation density was significantly higher than the void areas, as long as the test areas had the remedial composition applied.
The above-described embodiments of the present invention are not intended to limit the scope of the present invention, and the embodiments of the present invention are not limited thereto, and various other modifications, substitutions, or alterations made to the above-described structure of the present invention according to the general technical knowledge and conventional means of the art without departing from the basic technical idea of the present invention are intended to fall within the scope of the present invention.
Claims (6)
1. A remediation method for contaminated soil plants, characterized by: applying the remediation composition to soil and applying a barrier to the surface of the soil within the applied range, the barrier having an oxygen transmission rate of 2000ml/h m2 or less;
the repair composition comprises the following components in percentage by mass:
the fungi include: at least one of Phanerochaete chrysosporium, Quercus monarius, Profenoporus cinnabarinus, Alternaria mucilaginosa, Ceriporiopsis carbonaria, Trichoderma harzianum, Aspergillus oryzae, and Coriolus versicolor;
wherein the protein is calculated on a dry weight basis; the water content of the lignocellulose is less than 5%; the repairing composition is applied in an amount of 0.04-0.5kg/m2, wherein the fungus is required to be wrapped by lignocellulose and the water content is controlled below 5%.
2. The remediation method for contaminated soil plants according to claim 1, wherein: the plant species of the leguminous shrub ground product comprises at least one of mesquite, cassia tora, false cajan, amorpha fruticosa, faecium procumbens and silvery.
3. The remediation method for contaminated soil plants according to claim 1, wherein: the protein comprises potato protein and/or wheat protein.
4. The remediation method for contaminated soil plants according to claim 1, wherein: the fungus is a lignin peroxidase producing variety.
5. The remediation method for contaminated soil plants according to claim 1, wherein: the fungus is white rot fungus.
6. The remediation method for contaminated soil plants according to claim 1 or 2, wherein: the leguminous shrub grind is the root and/or stem of a plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911034192.7A CN110721996B (en) | 2019-10-29 | 2019-10-29 | Remediation method for contaminated soil plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911034192.7A CN110721996B (en) | 2019-10-29 | 2019-10-29 | Remediation method for contaminated soil plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110721996A CN110721996A (en) | 2020-01-24 |
| CN110721996B true CN110721996B (en) | 2022-02-01 |
Family
ID=69222310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911034192.7A Active CN110721996B (en) | 2019-10-29 | 2019-10-29 | Remediation method for contaminated soil plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110721996B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1173835A (en) * | 1995-09-25 | 1998-02-18 | 曾尼卡有限公司 | Anaerobic/aerobic decontamination of DDT contaminated soil by repeated anaerobic/aerobic treatments |
| CN1764717A (en) * | 2003-03-21 | 2006-04-26 | 科学与工业研究委员会 | A process for the isolation and acclimatization of bacteria for lignin degradation |
| CN101780466A (en) * | 2010-02-11 | 2010-07-21 | 仲恺农业工程学院 | Method for restoring soil polluted by heavy metal cadmium by using mesquite |
| CN104070062A (en) * | 2013-03-29 | 2014-10-01 | 天津地冠科技有限公司 | Microbial in-situ remediation method of heavy metal contaminated soil |
| CN104212822A (en) * | 2007-01-30 | 2014-12-17 | Bp法人北美有限公司 | Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100371094C (en) * | 2005-12-21 | 2008-02-27 | 中国石油化工股份有限公司 | In situ biological restoring method of petroleum polluted soil |
| CN108558461A (en) * | 2018-06-08 | 2018-09-21 | 合肥净龙环保科技有限公司 | A kind of planting nutrient solution and preparation method thereof increasing local soil nutrient |
-
2019
- 2019-10-29 CN CN201911034192.7A patent/CN110721996B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1173835A (en) * | 1995-09-25 | 1998-02-18 | 曾尼卡有限公司 | Anaerobic/aerobic decontamination of DDT contaminated soil by repeated anaerobic/aerobic treatments |
| CN1764717A (en) * | 2003-03-21 | 2006-04-26 | 科学与工业研究委员会 | A process for the isolation and acclimatization of bacteria for lignin degradation |
| CN104212822A (en) * | 2007-01-30 | 2014-12-17 | Bp法人北美有限公司 | Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them |
| CN101780466A (en) * | 2010-02-11 | 2010-07-21 | 仲恺农业工程学院 | Method for restoring soil polluted by heavy metal cadmium by using mesquite |
| CN104070062A (en) * | 2013-03-29 | 2014-10-01 | 天津地冠科技有限公司 | Microbial in-situ remediation method of heavy metal contaminated soil |
Non-Patent Citations (2)
| Title |
|---|
| 8种豆科灌木栽培种丛枝菌根真菌种类及分布;吴涛;《草业科学》;20160228;第210-216页 * |
| Recent developments in biodegradation of industrial;Muhammad Asgher;《Biodegradation》;20081231;第771-778页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110721996A (en) | 2020-01-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kohler et al. | The combination of compost addition and arbuscular mycorrhizal inoculation produced positive and synergistic effects on the phytomanagement of a semiarid mine tailing | |
| Wang et al. | Arbuscular mycorrhizal colonization alters subcellular distribution and chemical forms of cadmium in Medicago sativa L. and resists cadmium toxicity | |
| Miller et al. | Mycorrhizal mediated feedbacks influence net carbon gain and nutrient uptake in Andropogon gerardii | |
| Meier et al. | Interactive effect between Cu‐adapted arbuscular mycorrhizal fungi and biotreated agrowaste residue to improve the nutritional status of Oenothera picensis growing in Cu‐polluted soils | |
| Mench et al. | Phytomanagement and remediation of cu-contaminated soils by high yielding crops at a former wood preservation site: Sunflower biomass and ionome | |
| Liu et al. | Subsurface drip irrigation reduces cadmium accumulation of pepper (Capsicum annuum L.) plants in upland soil | |
| Memoli et al. | Short‐and long‐term effects of a single application of two organic amendments | |
| Takács et al. | Effect of metal non-adapted arbuscular mycorrhizal fungi on Cd, Ni and Zn uptake by ryegrass | |
| Guerrero‐Molina et al. | Elemental composition of strawberry plants inoculated with the plant growth‐promoting bacterium A zospirillum brasilense REC 3, assessed with scanning electron microscopy and energy dispersive X‐ray analysis | |
| Husna et al. | Stemphylium lycopersici and Stemphylium solani improved antioxidant system of soybean under chromate stress | |
| Thai et al. | Causes clarification of the soil aggregates stability on mulched soil. | |
| Duponnois et al. | The effects of organic amendments on the interactions between a nematophagous fungus Arthrobotrys oligospora and the root-knot nematode Meloidogyne mayaguensis parasitizing tomato plants | |
| Muche et al. | Evaluating slow pyrolysis of Parthenium hysterophorus biochar: perspectives to acidic soil amelioration and growth of selected wheat (Triticum aestivum) varieties | |
| Jarial et al. | Casing in mushroom beds–A review | |
| CN110721996B (en) | Remediation method for contaminated soil plants | |
| Jonsson et al. | The effect of mixing ground leaf litters to soil on the development of pitch pine ectomycorrhizal and soil arthropod communities in natural soil microcosm systems | |
| Al-Hakimi | Effect of salicylic acid on biochemical changes in wheat plants under khat leaves residues | |
| Doni et al. | Innovative system for biochemical monitoring of degraded soils restoration | |
| WO2009106777A2 (en) | Method for pollutant removal from soil contaminated by heavy metals | |
| Oliferchuk et al. | Changes in the structure of soil microscopic fungi in the territories of Yavoriv and Podorozhenie Sulfur Quarries | |
| Gibson et al. | Effects of microwave treatment of soil on growth of birch (Betula pendula) seedlings and infection of them by ectomycorrhizal fungi | |
| Ouatiki et al. | Inoculation of Pinus halepensis with the ectomycorrhizal fungi Scleroderma helps in phytoremediation of soil polymetallic pollution | |
| Li et al. | Arbuscular mycorrhizal fungi-mediated resistance to salt spray in Cinnamomum camphora seedlings enhanced by leaf functional traits | |
| Minaei et al. | Effects of de-awning and moisture content on husking characteristics of paddy in rubber-roll husker | |
| Kabir et al. | A bio-sustainable approach for reducing Eucalyptus tree-caused agricultural ecosystem hazards employing Trichoderma bio-sustained spores and mycorrhizal networks |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |