CN104067988A - Olfactometer for researching olfaction orientation behavior of insect for high-stick crop - Google Patents
Olfactometer for researching olfaction orientation behavior of insect for high-stick crop Download PDFInfo
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- CN104067988A CN104067988A CN201410288614.4A CN201410288614A CN104067988A CN 104067988 A CN104067988 A CN 104067988A CN 201410288614 A CN201410288614 A CN 201410288614A CN 104067988 A CN104067988 A CN 104067988A
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- 230000008786 sensory perception of smell Effects 0.000 title claims abstract description 13
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- 230000008635 plant growth Effects 0.000 claims abstract description 46
- 239000011521 glass Substances 0.000 claims abstract description 23
- 230000001681 protective effect Effects 0.000 claims abstract description 9
- 230000006399 behavior Effects 0.000 claims description 31
- 210000001503 joint Anatomy 0.000 claims description 14
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- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 claims 1
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Abstract
The invention provides an olfactometer for researching an olfaction orientation behavior of an insect for a high-stick crop. The olfactometer comprises a growth box made of organic glass, a bottom pad, an insect activity room, a netlike protective hood, a base and a communicating pipe, wherein a vent hole and a connecting hole are respectively formed in the two opposite side faces of the plant growth box; a movable door which can be opened and closed is arranged on a side face which is adjacent to the side face with the vent hole or the connecting hole; the bottom of the plant growth box is arranged on the bottom pad in a seamless butting mode; the insect activity room comprises sidewalls made of the organic glass; an opening in the bottom of the insect activity room is arranged on the base in the seamless butting mode, an opening in the top part thereof is covered with the protective hood, and the middle part thereof is provided with a clapboard serving as an insect carrying platform; multiple connecting holes in uniform distribution are formed in the sidewalls, located in the same horizontal plane, of the clapboard; the connecting hole of the plant growth box is connected with the connecting holes in the sidewalls of the insect activity room through the communicating pipe. The olfactometer for researching the olfaction orientation behavior of the insect for the high-stick crop is convenient to install and operate, and is convenient to observe an activity behavior of the insect in the activity room and a selective orientation behavior of the insect for the tested plant.
Description
Technical Field
The invention belongs to the technical field of agricultural and forestry pest behavior research instruments, and particularly relates to a smell meter for researching olfactory directional behaviors of insects on long-stalked crops.
Background
The insect has sensitive olfactory orientation and behavior reaction to host plant odor or insect pheromone, and the insect is very sensitive to tiny change of odor, and for a long time, in order to observe and measure the olfactory orientation and behavior reaction of the insect, people design and manufacture various types of olfactometers and wind tunnels, and commonly used are a Y-shaped olfactometer, a four-arm olfactometer, a single olfactometer and an antennal potentiometer. The Y-shaped olfactometer is the most commonly used olfactometer, but can only test the selective reaction of insects to 2 odor sources, and is also a small wind tunnel, can be used for testing the selective reaction of various insects such as lepidoptera adults, larvae, pest parasitic bees and the like to different odor sources, and plays an important role in researching the olfactory orientation and reaction behaviors of the insects. However, the Y-shaped olfactometer can only test 1 odor source at a time. The core of the four-arm olfactometer is a spherical glass movable chamber, 4 arms are arranged at the base part and connected with the movable chamber, an air outlet is arranged at the top part, and the bottled odorous source substances are trapped and mainly used for testing the behavioral response of small insects, can be used for testing the different concentrations of various odors or one odor of the insects, and can simultaneously test the behavioral response of the insects to the 4 odors. The single olfactometer is a glass tube with the length of 30cm and the diameter of 0.8cm, is divided into 6 chambers (0-5 chambers), and is mainly used for researching the olfactive tendency of small insects such as parasitic bees and diptera. After the Y-shaped olfactometer, the four-arm olfactometer and the single-wall olfactometer, the wind tunnel instrument is designed and manufactured according to the principle that insects fly against the wind towards an odor source, creates a space capable of simulating the flying activity of the insects in the field indoors, and is mainly used for testing the reaction of flying insects to odor substances. The antennal potentiostat is an electrophysiological technique for detecting the reaction of insects to chemical signals such as plant volatiles or insect pheromones, and when an air current blows onto the insect antennal, the electrode between the tip and the base of the antennal can record the potential change at the two ends of the antennal. An antennal potential instrument may also be used with gas chromatography, known as a combined antennal potential-gas chromatography instrument (GC-MS). However, the electroantennogram only detects the physiological reaction of insect antennae to compounds, and cannot determine the behavior activity of insects, but the behavior activity can only be determined through behavior experiments.
In summary, the olfactometer such as the currently used Y-shaped olfactometer, four-arm olfactometer, single olfactometer, wind tunnel and electroantennal potentiometer only aims at chemical substances of plants, namely, firstly collecting plant volatile matters, and then testing the olfactory reaction of insects to the collected volatile matters, or selecting plant seedlings as odor sources to test, or only testing 1 plant at a time, and only being seedlings, plantlets or detached plant tissues, but not being capable of testing plants after high-stem crops or seedlings, and also being capable of testing the olfactory reaction of multi-head insects to various odor sources, and even being incapable of testing the olfactory reaction of insects to the crop diversity configuration system odor sources.
Disclosure of Invention
The invention aims to provide a olfactometer for researching olfaction orientation behaviors of insects on long-staled crops, and aims to overcome the defects in the background technology.
The olfactometer for researching olfaction orientation behavior of insects on long-stalk crops comprises a plant growth box made of organic glass, a bottom pad, an insect activity chamber, a net-shaped shield, a base and a communicating pipe, wherein the plant growth box is made of organic glass;
the two opposite side surfaces of the plant growth box are respectively provided with a vent hole and a connecting hole, and the side surface adjacent to the plant growth box provided with the vent hole or the connecting hole is provided with a movable door capable of being opened and closed; the bottom of the plant growth box is arranged on the bottom pad in a seamless butt joint mode;
the insect activity room comprises a side wall formed by organic glass in a surrounding manner; the bottom opening of the insect activity chamber is arranged on the base in a seamless butt joint mode, the top opening is covered by the protective cover, the middle part is provided with a partition plate used as an insect carrying platform, and a plurality of connecting holes are uniformly distributed on the side wall which is positioned on the same plane with the partition plate;
the connecting hole of the plant growth box is connected with a connecting hole on the side wall of the insect activity chamber through a communicating pipe.
Preferably, the olfactometer further comprises a vent pipe, a flow meter, a filter bottle and an air pump; the flow meter, the filter flask and the air pump are sequentially arranged on the vent pipe according to the air flow direction, and the air inlet end of the vent pipe is in butt joint with the vent hole of the plant growth box.
Preferably, the olfactometer further comprises a nesting for butt-jointing and communicating the two communicating pipes; wherein,
the connecting holes of the plant growth box are butted with a section of communicating pipe, and the connecting holes on the side wall of the insect movable chamber are respectively butted with a section of communicating pipe; the communicating pipe on the plant growth box is communicated with a section of communicating pipe on the side wall of the insect activity chamber through nesting.
Preferably, the heelpiece is movable cuboid organic glass board, and each edge of heelpiece is equipped with the recess that a rubber materials made respectively, each lateral wall bottom of vegetation case is inserted sealed butt joint in the recess.
Preferably, the number of the plant growth boxes is four, the plant growth boxes are all made of 2.5 mm-thick organic glass into a cuboid, and the size of the plant growth boxes is 35cm in length, 70.0cm in width and 200cm in height; the number of the connecting holes formed in the side wall of the insect activity chamber is 4.
Preferably, the insect activity room is a cylinder formed by organic glass with the thickness of 2.0mm, and the specification is 20cm in diameter and 20cm in height.
Preferably, the partition board is arranged at the position 5cm higher from the bottom of the side wall of the insect activity chamber.
Compared with the defects and shortcomings of the prior art, the invention has the following beneficial effects:
(1) the plant growth box is 200cm high, and can meet the requirements of high-stalk crops with higher plants, such as high-stalk crops of corn, sugarcane, sorghum and the like, and even some woody plant seedlings and the like.
(2) The number of the plant growth boxes is 4, each growth box is 35cm in length, 70.0cm in width and 200cm in height, and 1-3 crops or plants can be placed in each growth box at the same time; therefore, the olfactory orientation and reaction of the tested insects to the environment existing in a single crop or two crops can be observed after the tested insects are placed in an insect activity room, and the olfactory orientation and reaction can be used for researching the influence of crop diversity planting on the selection behavior of the insect hosts.
(3) The insect activity room of the invention has large space, can ensure that insects of the insects fly, crawl, land and inhabit, and can place a plurality of insects at one time.
(4) The invention has convenient installation and operation, the host selection device can be detached from the plant growth box and the insect activity room, is convenient to store, and can reduce the influence of the odor in the external environment on the insect behavior during application.
(5) The invention is convenient for observation and does not influence the light required by the growth of the plants. Because the device is made of transparent organic glass with the thickness of 2.5mm, the activity behavior of the insects in the activity room and the selection trend movement behavior of the tested plants can be clearly observed.
Drawings
FIG. 1 is a schematic structural diagram of a top view of a smell meter for researching olfactory directional behaviors of insects on long-stalked crops according to the invention;
FIG. 2 is a schematic front structure diagram of an assembled insect activity chamber and a plant growth chamber in the olfactometer for researching olfaction orientation behavior of insects on long-stalked crops.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in fig. 1-2, wherein fig. 1 is a schematic structural diagram of a smell meter for researching olfactory orientation behavior of insects on long-stalked crops in a top view state; FIG. 2 is a schematic front structure diagram of an assembled insect activity chamber and a plant growth chamber in the olfactometer for researching olfaction orientation behavior of insects on long-stalked crops.
A olfactometer for researching olfaction orientation behavior of insects to long-staled crops comprises a plant growth box 1 made of organic glass, a bottom pad 2, an insect activity chamber 3, a net-shaped shield 4, a base 5 and a communicating pipe 6; two opposite side surfaces of the plant growth box 1 are respectively provided with a vent hole 7 and a connecting hole 8, and the side surface adjacent to the vent hole 7 or the connecting hole 8 is provided with a movable door 9 capable of being opened and closed; the bottom of the plant growth box 1 is arranged on the bottom pad 2 in a seamless butt joint mode;
the insect activity room 3 comprises a side wall 10 formed by organic glass; the bottom opening of the insect activity chamber 3 is arranged on the base 5 in a seamless butt joint mode, the top opening is covered by the protective cover 4, the middle part is provided with a partition plate 11 used as an insect carrying platform, and a plurality of connecting holes 12 are uniformly distributed on the side wall 10 which is positioned on the same plane with the partition plate 11;
the connecting hole 8 of the plant growth box 1 is connected with a connecting hole 12 on the side wall 10 of the insect activity chamber 3 through a communicating pipe 6.
In the embodiment of the invention, more specifically, according to the size and the range of the insects, the insect activity chamber 3 is 20cm in diameter and 20cm in height, and the partition 11 is arranged on the side wall 10 of the insect activity chamber 3 at a position 5cm away from the bottom. For the convenience of observation, the insect activity room 3 is a cylinder surrounded by 2.0mm thick organic glass.
In the embodiment of the invention, more specifically, according to the plant growth needs and the actual experiment needs, the number of the plant growth boxes 1 is four, and the specifications are 35cm in length, 70.0cm in width and 200cm in height; for the convenient observation and do not influence the required light of vegetation, vegetation case 1 is the cuboid of making with the organic glass of 2.5mm thickness. At this time, the number of the connecting holes 12 arranged on the side wall 10 of the insect activity chamber 3 is 4, and the connecting holes 12 are respectively butted with the connecting holes 8 on the four plant growth boxes 1 through a communicating pipe 6.
In the embodiment of the invention, more specifically, in order to facilitate the disassembly and assembly of the whole device, the olfactometer further comprises a nest 13 for butt-jointing and communicating the two communicating pipes; wherein, the connecting hole 8 of the plant growth box 1 is butted with a section of communicating pipe, and the connecting holes 12 on the side wall 10 of the insect activity room 3 are respectively butted with a section of communicating pipe; the communicating pipe on the plant growth box 1 is communicated with a section of communicating pipe on the side wall 10 of the insect activity chamber 3 through a nesting 13.
In the embodiment of the invention, more specifically, in order to facilitate the disassembly and assembly of the plant growth box 1, the bottom pad 2 is a movable cuboid organic glass plate, each edge of the bottom pad 2 is respectively provided with a groove made of a rubber material, and the bottom of each side wall of the plant growth box 1 is inserted into the groove to be in sealing butt joint.
In the practical application process of the invention, different kinds of crops can be planted in the four plant growth chambers 1 respectively according to needs, each growth chamber is 35cm long, 70.0cm wide and 200cm high, and 1-3 crops or plants can be placed in each growth chamber at the same time; the plant growth box is 1 cm high and 200cm high, and can meet the requirements of high-stalk crops with higher plants, such as high-stalk crops of corn, sugarcane, sorghum and the like, and even some woody plant seedlings and the like.
The tested insects are put between the partition board 6 and the protective cover 4 in the insect activity room 3, the protective cover 4 is a protective layer made of a nylon net and used for preventing the insects from escaping, and the partition board 6 in the middle of the height of the activity room ensures that the insects land and inhabit. The space between the protective cover 4 and the clapboard 6 is large enough, and a plurality of insects can be placed inside the protective cover simultaneously, which is beneficial to the mixing of the insect activity and the air flow of the odor information substance, can also satisfy the free activity of the insects, and can ensure that the insects of the insects fly and crawl.
After the test insects are put in, the olfactory orientation and reaction of the insects to a single crop or two crops in the environment can be observed through the organic glass, and the invention is made of the transparent organic glass with the thickness of 2.5 or 2.0mm, so that the activity behavior of the insects in a moving room and the selection trend motion behavior of the test plants can be clearly observed, and the invention can be used for researching the influence of crop diversity planting on the selection behavior of insect hosts.
The invention has convenient installation and operation, the plant growth box and the insect activity room can be detached and are convenient to store, and meanwhile, the influence of the odor in the external environment on the insect behavior can be reduced when the invention is applied.
In a further implementation, in order to better control the gas cross-linking strength, in the embodiment of the present invention, the olfactometer further includes a ventilation pipe 14, a flow meter 15, a filter bottle 16 and an air pump 17; the flow meter 15, the filter bottle 16 and the air pump 17 are sequentially arranged on the vent pipe 14 according to the air flow direction, and the air inlet end of the vent pipe 14 is in butt joint with the vent hole 7 of the plant growth box 1.
In the embodiment of the present invention, the filter bottle 16 includes a glass bottle and a rubber stopper, the rubber stopper is provided with an air inlet and an air outlet, one section of the vent pipe 14 is provided with a flow meter 15 and then is in butt joint with the air inlet, and the other section of the vent pipe 14 is in butt joint with the air outlet and then is connected with the air pump 17. The air pump and the flowmeter are purchased from the household appliance market.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. A olfactometer for researching olfaction orientation behavior of insects on long-stalk crops is characterized by comprising a plant growth box made of organic glass, a bottom pad, an insect activity chamber, a net-shaped shield, a base and a communicating pipe;
the two opposite side surfaces of the plant growth box are respectively provided with a vent hole and a connecting hole, and the side surface adjacent to the plant growth box provided with the vent hole or the connecting hole is provided with a movable door capable of being opened and closed; the bottom of the plant growth box is arranged on the bottom pad in a seamless butt joint mode;
the insect activity room comprises a side wall formed by organic glass in a surrounding manner; the bottom opening of the insect activity chamber is arranged on the base in a seamless butt joint mode, the top opening is covered by the protective cover, the middle part is provided with a partition plate used as an insect carrying platform, and a plurality of connecting holes are uniformly distributed on the side wall which is positioned on the same plane with the partition plate;
the connecting hole of the plant growth box is connected with a connecting hole on the side wall of the insect activity chamber through a communicating pipe.
2. The olfactometer for researching olfaction-oriented behaviors of insects on long-stalked crops as claimed in claim 1, wherein the olfactometer further comprises a breather pipe, a flow meter, a filter bottle and an air pump; the flow meter, the filter flask and the air pump are sequentially arranged on the vent pipe according to the air flow direction, and the air inlet end of the vent pipe is in butt joint with the vent hole of the plant growth box.
3. The olfactometer for researching olfaction-directed behavior of insects on long-stalked crops as claimed in claim 2, wherein the olfactometer further comprises a nest for butt-jointing and connecting the two communicating pipes; wherein,
the connecting holes of the plant growth box are butted with a section of communicating pipe, and the connecting holes on the side wall of the insect movable chamber are respectively butted with a section of communicating pipe; the communicating pipe on the plant growth box is communicated with a section of communicating pipe on the side wall of the insect activity chamber through nesting.
4. The olfactometer for researching olfaction orientation of insects to long-stalked crops as claimed in claim 3, wherein the bottom pad is a movable cuboid organic glass plate, each edge of the bottom pad is provided with a groove made of rubber material, and the bottom of each side wall of the plant growth box is inserted into the groove for sealing and butt joint.
5. The olfactometer for researching olfaction orientation behaviors of insects on long-stalked crops as claimed in claim 4, wherein the number of the plant growth boxes is four, and the four plant growth boxes are all made of organic glass with the thickness of 2.5mm into a cuboid, and the specifications are 35cm in length, 70.0cm in width and 200cm in height; the number of the connecting holes formed in the side wall of the insect activity chamber is 4.
6. The olfactometer for researching olfaction orientation behavior of insects on long-stalked crops as claimed in claim 5, wherein the insect activity chamber is a cylinder surrounded by 2.0mm thick plexiglass, and the specification is 20cm in diameter and 20cm in height.
7. The olfactometer for researching olfactory orientation of insects on long-stalked crops as claimed in claim 6, wherein said partition is provided at a height of 5cm from the bottom of the side wall of the insect activity chamber.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410288614.4A CN104067988B (en) | 2014-06-25 | 2014-06-25 | A kind of study the insecticide olfactometer to long-stalked crops olfactory sensation orientation behaviour |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410288614.4A CN104067988B (en) | 2014-06-25 | 2014-06-25 | A kind of study the insecticide olfactometer to long-stalked crops olfactory sensation orientation behaviour |
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| CN104067988A true CN104067988A (en) | 2014-10-01 |
| CN104067988B CN104067988B (en) | 2016-09-07 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110934116A (en) * | 2019-12-09 | 2020-03-31 | 海南省粮油科学研究所 | Insect smell sense testing device and method for detecting insect smell sense trapping effect of material |
| CN111374096A (en) * | 2020-04-29 | 2020-07-07 | 金华市农业科学研究院 | Device for preference selection of feed of galleria mellonella larvae and application |
| CN113171199A (en) * | 2021-05-08 | 2021-07-27 | 湖州师范学院 | Insect corner contact pliers |
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| CN113171199A (en) * | 2021-05-08 | 2021-07-27 | 湖州师范学院 | Insect corner contact pliers |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104067988B (en) | 2016-09-07 |
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