CN103235092A - Testing device for indoor toxicity of insecticide to shoot-species piercing-sucking pests - Google Patents
Testing device for indoor toxicity of insecticide to shoot-species piercing-sucking pests Download PDFInfo
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- CN103235092A CN103235092A CN2013101499243A CN201310149924A CN103235092A CN 103235092 A CN103235092 A CN 103235092A CN 2013101499243 A CN2013101499243 A CN 2013101499243A CN 201310149924 A CN201310149924 A CN 201310149924A CN 103235092 A CN103235092 A CN 103235092A
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Abstract
The invention relates to a testing device for indoor toxicity of an insecticide to shoot-species piercing-sucking pests. The testing device comprises a bioassay hood, a bottom cover and a bioassay hood net cover, wherein two ends of the bioassay hood are penetrated. The testing device is characterized in that the bottom cover is connected to the lower part of the bioassay hood, the bioassay hood net cover is connected to the upper part of the bioassay hood, a cylindrical container is fixedly arranged on the middle part of the bottom cover in the bioassay hood, and a water-absorbing cylindrical object for fixing and moisturizing shoots is filled in the cylindrical container. The device disclosed by the invention is simple in manufacture, convenient to use and wide in applicable range, not only is suitable for testing the indoor toxicity of the insecticide to the shoot-species piercing-sucking pests, but also can be used for remotely carrying piercing-sucking living insects and maintaining the completeness and the survival rate of the insects.
Description
Technical field
The present invention relates to a kind of pesticide to branch class sucking pest Toxicity Determination new equipment, be applied to the indoor biological activity determination of agricultural chemicals, also can be applicable to carrying at a distance of pierce-suck type live body insect.
Background technology
Usually need in the novel pesticide development process to carry out agricultural chemicals to the biological activity determination of insect, in the scientific research process relevant with the pesticide research field such as pest resistance to insecticide monitoring, all relate to the work of agricultural chemicals Toxicity Determination, the assay method of " People's Republic of China's agricultural industry criteria " relevant agricultural chemicals (pesticide) indoor bioassay test rule regulation has drop method at present, press from both sides malicious blade method, fumigating system, the blade immersion method, dip method, wherein the insect of sucking mouth parts is mainly adopted drop method, immersion method and dip method are measured continuously, but, these criterions have just been stipulated experimental insect, the standard of experimental plant organization material and experimental pharmacy relevant treatment, the plant tissue material nutrition supply that how to guarantee after experiment is handled to exsomatize is normally got the food aspect and is not then had clear and definite standard to guarantee experimental insect, particularly carry out pesticide to branch class sucking pest Toxicity Determination in, isolated experiment plant tissue material is preserved moisture, nutrition supply and the test space environment that provides experimental insect to suit activity are Success in Experiment and the key link that obtains accurate data.At present, in the scientific experiment process of relevant agricultural chemicals toxicity test, all be to adopt filter paper after the experimental plant organization material chemicals treatment, absorbent cotton, water-absorbing materials such as flower mud are implemented simple and easy moist keeping measures, place plastic bottle or double dish that experimental insect is carried out simple and easy breeding observing then, but all there is obvious defects in these methods at present: at first material which can retain moisture does not have and the tight immovable combination of test container, observe in experiment and often to cause material which can retain moisture to move in the mobile test container processes and cause the plant tissue material to come off from material which can retain moisture, very easily cause experimental insect to be extruded and dead; And adsorbed nutrition and the moisture of these material which can retain moisture is limited, can only play the short time effect, can't continue to satisfy preserving moisture and the nutrition supply effect of isolated experiment plant tissue material the long period, often cause stripped plant branch tissue shortage nutrition and moisture to cause plant tissue withered easily, cause experimental insect to lack food and death; In addition, currently used test container can't provide ventilated clog-free activity space environment for experimental insect, causes the too high oxygen that lacks again of humidity in the test container, causes the phenomenon of a large amount of deaths by suffocation of experimental insect; Thereby cause pesticide Toxicity Determination experimental result inaccurate even cause the failure of an experiment, great amount of manpower and material resources and financial resources have been wasted, in addition, the source of nutrition and the material which can retain moisture that also have the agar layer utilized to cultivate as the plant leaf blade short time of exsomatizing, but the operating process of this method is comparatively numerous tired, also can't the activity of real simulation insect field and the virtual condition of getting food.Above-mentioned these problems, what show in branch class sucking pest Toxicity Determination experimentation is particularly evident, is badly in need of a kind ofly overcoming the new equipment of above-mentioned defective to satisfy pesticide to branch class sucking pest Toxicity Determination research work.In addition, in pest resistance to insecticide study on monitoring process, the insect that needs to gather zones of different carries out Toxicity Determination, this relates to the problem that experimental insect carries at a distance, if and experimental insect is carrying shortage food and the ventilated phenomenon that also can cause its energy decline or mass mortality in the process at a distance, certainly will cause the experimental insect that carries at a distance can not satisfy the laboratory experiment requirement, thus cause the experiment can't normally carry out or experimental result inaccurate.Therefore, are research purposes of the present invention at the problems referred to above.
Summary of the invention
The object of the present invention is to provide a kind of pesticide to branch class sucking pest Toxicity Determination device, helping to solve present pesticide is extruded easily to the experimental insect that branch class sucking pest Toxicity Determination exists, the plant branch tissue that exsomatizes lacks nutrition and the moisture supply causes dry dehydration even withered, thereby experimental insect lacks the problem that ventilated clog-free activity space environment causes the inaccurate or the failure of an experiment of experimental result, this device invention has solved that experimental insect is extruded easily in the pesticide Toxicity Determination, exsomatizing, plant branch tissue can not effectively be fixed and continual and steady nutrition and moisture is supplied with, the space environment condition of ventilated accessible activity is provided for experimental insect, the virtual condition of having simulated the activity of insect field comparatively truly and having got food has improved accuracy and the reliability of pesticide to branch class sucking pest Toxicity Determination result.
Technical program of the present invention lies in:
A kind of pesticide is to branch class sucking pest Toxicity Determination device, comprise living cover, bottom and the living cover square of surveying surveyed that two ends are run through, its feature exists: the described bottom of surveying cover of giving birth to is connected with bottom, the described top of surveying cover of giving birth to is connected with the living cover square of surveying, be positioned at living the survey on the bottom middle part of covering and be installed with column container, be filled with a water absorptivity column in order to fixing and the branch of preserving moisture in the described column container.
Wherein, described giving birth on the sidewall of surveying cover offers the gauze window.
The described middle part of surveying the cover square of giving birth to offers the gauze window.
Described water absorptivity column comprises the first half and Lower Half, and closely paste mutually with the column container inboard in the outside of the described first half, and the outside of described Lower Half and column container inner distance 0.5 ~ 1.0cm form the spatial joint clearance in order to the splendid attire nutrient solution.
Described water absorptivity column is the decorating film water-absorbing material of sponge or flower mud.
Described top that give birth to survey cover is the enclosure-type socket joint between the bottom of covering and the bottom and is connected with giving birth to survey between the cover square and give birth to survey.
Described bottom, give birth to survey the cover square, give birth to survey cover, column container is made by transparent plastic material.
The invention has the advantages that:
1. this new equipment cost is low, making is simple, easy to use;
2. the minitype column shape container on this new equipment bottom provides nutrient source and moisture source as the nutrient solution container for testing the plant branch that exsomatizes, and guarantees to keep in the branch long period freshness, and the minimizing experimental insect is murderous phenomenon because food nutrition lacks;
3. the minitype column shape container on this new equipment bottom is embedded with the decorating film absorbent material of bar shape sponge or flower mud, organize the fixing and moisture-keeping function of branch as the plant branch that exsomatizes, solved in the present Toxicity Determination process because test container moves and cause the plant tissue material to come off and material which can retain moisture moves and causes experimental insect to be extruded deadly phenomenon, also simulated the virtual condition that the insect field is movable and get food comparatively truly;
4. the survey cover column side face of giving birth to of this new equipment offers the gauze window, be conducive to ventilated, guaranteed that the residing test environment of experimental insect has stable air humidity and oxygen, for experimental insect provides an accessible ventilated activity space environment, reduced the phenomenon of experimental insect because of death by suffocation;
5. the survey cover square of giving birth to of this new equipment is connected for the enclosure-type socket joint with the living cover of surveying, and conveniently experimental insect is introduced living observation of surveying cover and experimentation, and is ventilated window of experimental insect increase;
6. this new equipment is suitable for pesticide to the Toxicity Determination use of branch class sucking pest, also can be used for integrality and survival rate that piercing sucking insect carried and guaranteed insect at a distance.
Description of drawings
Fig. 1 is the monnolithic case structural representation of the embodiment of the invention.
Fig. 2 is that the A-A of Fig. 1 is to the sectional structure synoptic diagram.
Embodiment
For above-mentioned feature and advantage of the present invention can be become apparent, embodiment cited below particularly, and conjunction with figs. are described in detail below.
With reference to figure 1 and Fig. 2, the present invention relates to a kind of pesticide to branch class sucking pest Toxicity Determination device, giving birth to of comprising that two ends run through surveyed cover 1, bottom 2 and given birth to and survey cover square 3, the described bottom of surveying cover 1 of giving birth to is connected with bottom 2, the described top of surveying cover 1 of giving birth to is connected with the living cover square 3 of surveying, be positioned on the bottom middle part that give birth to survey cover 1 and be installed with column container 4, be filled with one in the described column container 4 in order to fix and the water absorptivity column 5 of the branch 7 of preserving moisture.
Above-mentioned giving birth on the sidewall of surveying cover 1 offers gauze window 1-1.
The above-mentioned middle part of surveying cover square 3 of giving birth to offers gauze window 3-1.
Above-mentioned water absorptivity column 5 comprises the first half and Lower Half, and closely paste mutually with the column container inboard in the outside of the described first half, and the outside of described Lower Half and column container inner distance 0.5 ~ 1.0cm form the spatial joint clearance in order to splendid attire nutrient solution 6.
Above-mentioned water absorptivity column 5 is the decorating film water-absorbing material of sponge or flower mud.
Above-mentioned top that give birth to survey cover 1 is the enclosure-type socket joint between 1 bottom and the bottom 2 and is connected with giving birth to survey between the cover square 3 and give birth to survey to cover.
Above-mentioned bottom 2, give birth to survey cover square 3, give birth to survey cover 1, column container 4 is made by transparent plastic material.
Cylindrical tube body or the square body container that cover 1 is made for transparent plastic material surveyed in giving birth to of this new equipment, and volume is with 500-1000 cm
3Be advisable, can adjust the size of new equipment main volume according to actual test sample book size, by bottom 2, give birth to survey cover 1 and give birth to and survey the inside subject space that cover square 3 forms and get the food space environment as the activity of toxicity test experimental insect.
For convenience of nutrient solution splendid attire and placement plant branch water absorptivity column, column container 4 uprightly is fixedly mounted on the inboard centre position of bottom 2 for non-dismountable formula.
For the phenomenon that guarantees that the experimentation branch can effectively be fixed on the material which can retain moisture and avoid material which can retain moisture to move causing experimental insect to be extruded, the water absorptivity column 5 of described new equipment is the decorating film water-absorbing material of bar shape, its integral body is the indivisible five equilibrium first half and Lower Half, the upper end of the water absorptivity column first half of bar shape flushes with bar shape container 4 openends, the Lower Half lower end flushes with bar shape container 4 bottoms, closely paste mutually with bar shape container 4 inboards on the bottom in the outside of the water absorptivity column first half, the inboard spacing 0.5-1.0cm of the outside of Lower Half and column container 4, as splendid attire nutrient solution space, for providing lasting nutrition and moisture, water absorptivity column 5 supplies with.
Cleaning and the repeated use of experimental implementation and device for convenience, bottom 2 inner edges of described new equipment are connected for the enclosure-type socket joint with the bottom outer rim of giving birth to survey cover 1.
In order to guarantee that experimental insect is in suitable humidity and ventilative environmental baseline, the side of surveying cover 1 of giving birth to of described new equipment offers 3 with gauze window 1-1.
For the aspect experimental insect being introduced the living cover 1 of surveying, and prevent that experimental insect in the experimentation from escaping and the observation of experimentation, the survey cover square 3 of giving birth to of this new equipment is connected for the enclosure-type socket joint with the top end opening of giving birth to survey cover 1, giving birth to the middle body of surveying cover square 3 is gauze window 3-1, also increases a ventilated window for experimental insect.
In experimentation, a certain amount of nutritive water solution of packing in the column container on this new equipment bottom, the water absorptivity column soaked into nutritive water solution and embed in the column container on the bottom, to insert with insecticide treated stripped plant branch on the water absorptivity column, to give birth to then and survey cover insertion bottom, make to give birth to survey to cover and closely be connected with bottom, survey cover upper end open introducing experimental insect from giving birth to, to give birth to then and survey the cover square and insert to give birth to and survey the cover upper end and make it tight connection, at last whole new equipment is moved in the growth cabinet of the temperature and humidity condition that meets experimental design, by the dead quantity of experimental design requirement timing observed and recorded experimental insect.
The above only is preferred embodiment of the present invention, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to covering scope of the present invention.
Claims (7)
1. a pesticide is to branch class sucking pest Toxicity Determination device, comprise living cover, bottom and the living cover square of surveying surveyed that two ends are run through, its feature exists: the described bottom of surveying cover of giving birth to is connected with bottom, the described top of surveying cover of giving birth to is connected with the living cover square of surveying, be positioned at living the survey on the bottom middle part of covering and be installed with column container, be filled with a water absorptivity column in order to fixing and the branch of preserving moisture in the described column container.
2. a kind of pesticide according to claim 1 is characterized in that branch class sucking pest Toxicity Determination device: described giving birth on the sidewall of surveying cover offers the gauze window.
3. a kind of pesticide according to claim 1 and 2 is characterized in that branch class sucking pest Toxicity Determination device: the described middle part of surveying the cover square of giving birth to offers the gauze window.
4. a kind of pesticide according to claim 3 is to branch class sucking pest Toxicity Determination device, it is characterized in that: described water absorptivity column comprises the first half and Lower Half, closely paste mutually with the column container inboard in the outside of the described first half, the outside of described Lower Half and column container inner distance 0.5 ~ 1.0cm form the spatial joint clearance in order to the splendid attire nutrient solution.
According to claim 1 or 4 described a kind of pesticides to branch class sucking pest Toxicity Determination device, it is characterized in that: described water absorptivity column is the decorating film water-absorbing material of sponge or flower mud.
6. a kind of pesticide according to claim 5 is characterized in that branch class sucking pest Toxicity Determination device: described top that give birth to survey cover is the enclosure-type socket joint between the bottom of covering and the bottom and is connected with giving birth to survey between the cover square and give birth to survey.
According to claim 1 or 6 described a kind of pesticides to branch class sucking pest Toxicity Determination device, it is characterized in that: described bottom, give birth to survey the cover square, give birth to survey cover, column container is made by transparent plastic material.
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CN2013101499243A CN103235092A (en) | 2013-04-26 | 2013-04-26 | Testing device for indoor toxicity of insecticide to shoot-species piercing-sucking pests |
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CN2013101499243A CN103235092A (en) | 2013-04-26 | 2013-04-26 | Testing device for indoor toxicity of insecticide to shoot-species piercing-sucking pests |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108902052A (en) * | 2018-06-29 | 2018-11-30 | 中国农业大学 | A kind of method of cotten aphid indoor bioassay |
CN109845705A (en) * | 2019-03-26 | 2019-06-07 | 云南农业大学 | The small-sized parasitic bee imago stomach toxicity of one kind, Contact toxicity measurement device and application method |
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JPH07255323A (en) * | 1994-03-18 | 1995-10-09 | Kubota Corp | Method for breeding natural enemy insect |
CN201640214U (en) * | 2010-03-26 | 2010-11-24 | 中国检验检疫科学研究院 | Insect survival test tank applied in the soil environment |
CN202664022U (en) * | 2012-08-07 | 2013-01-16 | 云南省农业科学院生物技术与种质资源研究所 | Device suitable for insect feeding and biological observation |
CN202814968U (en) * | 2012-07-05 | 2013-03-20 | 浙江省农业科学院 | Device for testing drug resistance of rice planthopper |
CN202873587U (en) * | 2012-10-12 | 2013-04-17 | 北京市农林科学院 | Device performing bioassays on living plant |
CN203191364U (en) * | 2013-04-26 | 2013-09-11 | 福建省农业科学院植物保护研究所 | Indoor toxicity measuring device for insecticide against shoot piercing-sucking pests |
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2013
- 2013-04-26 CN CN2013101499243A patent/CN103235092A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH07255323A (en) * | 1994-03-18 | 1995-10-09 | Kubota Corp | Method for breeding natural enemy insect |
CN201640214U (en) * | 2010-03-26 | 2010-11-24 | 中国检验检疫科学研究院 | Insect survival test tank applied in the soil environment |
CN202814968U (en) * | 2012-07-05 | 2013-03-20 | 浙江省农业科学院 | Device for testing drug resistance of rice planthopper |
CN202664022U (en) * | 2012-08-07 | 2013-01-16 | 云南省农业科学院生物技术与种质资源研究所 | Device suitable for insect feeding and biological observation |
CN202873587U (en) * | 2012-10-12 | 2013-04-17 | 北京市农林科学院 | Device performing bioassays on living plant |
CN203191364U (en) * | 2013-04-26 | 2013-09-11 | 福建省农业科学院植物保护研究所 | Indoor toxicity measuring device for insecticide against shoot piercing-sucking pests |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108902052A (en) * | 2018-06-29 | 2018-11-30 | 中国农业大学 | A kind of method of cotten aphid indoor bioassay |
CN108902052B (en) * | 2018-06-29 | 2021-04-30 | 中国农业大学 | Indoor biological assay method for cotton aphids |
CN109845705A (en) * | 2019-03-26 | 2019-06-07 | 云南农业大学 | The small-sized parasitic bee imago stomach toxicity of one kind, Contact toxicity measurement device and application method |
CN109845705B (en) * | 2019-03-26 | 2024-06-04 | 云南农业大学 | Small parasitic wasp adult stomach toxicity and contact toxicity measuring device and using method |
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Application publication date: 20130807 |